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Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB 黑孜然抗结核分枝杆菌H37RV和耐多药结核
Pub Date : 2021-06-30 DOI: 10.22373/ekw.v7i1.9335
M. Masri, Cut Muthiadin, M. Masita, T. Cahyanto, L. Lianah, R. Rusny, S. Tridesianti
Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of  N. sativa seed extract in inhibiting the growth of  M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan.  Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua  strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%,  tetapi resisten terhadap  ekstrak N. sativa konsentrasi 1 dan 3%.
摘要:结核病(TB)是由结核分枝杆菌引起的传染性传染病。每年有1000万人患结核病。虽然结核病是一种可预防和可治疗的疾病,但每年仍有150万人死于结核病。继续寻求替代治疗方法,并继续鼓励使用最新的结核病药物进行治疗。黑孜然(Nigella sativa)种子含有含有活性化合物的精油,如百里香对苯二酚、油树脂、类黄酮、生物碱、皂苷、单宁和萜类,这些化合物可以作为抗菌药物。本研究旨在确定芥蓝种子提取物对结核分枝杆菌H37RV和耐多药结核(MDR-TB)生长的抑制敏感性。本研究采用显微观察和药敏试验(MODS)方法。以70%甲醇为溶剂,采用浸渍法提取芥蓝。结果表明,结核分枝杆菌H37RV和耐多药结核分枝杆菌对浓度为5%和10%的苜蓿野蓟提取物敏感,对浓度为1%和3%的苜蓿野蓟提取物耐药。摘要:结核(TB) adalah penyakit menular yang disebabkan oleh巴克特里结核分枝杆菌。Penyakit ini menimbulkan dampak kematian yang cuup mengkhawatirkan。Penyakit tersebut dapat dicegah dan diobati。黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳,黑木耳。结核分枝杆菌H37RV与耐多药结核(MDR-TB)。孟古那坎方法:显微观察及药敏试验。黄芪、黄芪、黄芪、黄芪、黄芪、黄芪、黄芪、黄芪、黄芪。哈氏结核分枝杆菌H37RV株为TB-MDR,克氏结核分枝杆菌H37RV株为敏感株,耐药株占10%,耐药株占3%。
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引用次数: 1
Characterization of Organic Polymer Monolith Columns Containing Ammonium Quarternary As Initial Study For Capillary Chromatography 含季铵盐有机聚合物整体柱的表征及其毛细管色谱的初步研究
Pub Date : 2021-06-30 DOI: 10.22373/ekw.v7i1.8764
A. Rahayu, S. Jamilatun, J. A. Fajri, L. Lim
Abstract: The polymerization process with a simple step has become the centre of attention of several researchers. Various polymers have been developed, although in general, they use polymerization with a post-modification method. A quaternary ammonium monolith organic polymer has been prepared using a simple single thermal method in this research. 2-(Methacryloyloxy)-N,N,N-trimethylethanaminium chloride was as the monomer, and ethylene dimethacrylate was as crosslinker. The polymerization proceeded in fused-silica capillary (100 mm, 0.32 mm i.d. x 0.45 mm o.d.) using a one-pot approach method. To achieve the perfect macropores, isopropyl alcohol, PEG 400, and ethanol were used as porogen. Characterization of the surface morphology was carried out using a Scanning Electron Microscope (SEM), and the existence of an amine group was characterized by Fourier Transform Infrared Spectroscopy (FTIR). The distribution size of pores in the polymer was in the range of 1.29 to 3.33 µm.Abstrak: Polimerisasi dengan proses yang sederhana dan simpel menjadi pusat perhatian beberapa peneliti. Berbagai macam polimer telah dikembangkan, akan tetapi pada umumnya menggunakan polimerisasi dengan metode post-modification. Pada penelitian ini, polimer organik yang mengandung amonium kuartener dalam bentuk monolit dengan polimerisasi yang menggunakan suhu tunggal dan sederhana telah dilakukan. 2-(Methacryloyloxy)-N,N,N-trimethylethanaminium chloride digunakan sebagai monomer dan ethylene dimethacrylate sebagai crosslinker. Polimerisasi dilakukan dengan metode one-pot aaproach di dalam kapiler silika (100 mm, 0,32 mm i.d. x 0,45 mm o.d.). Untuk mendapatkan makropori yang sempurna, isopropil alkohol, PEG 400 dan etanol digunakan sebagai porogen. Karakterisasi morfologi permukaan dilakukan dengan menggunakan Scanning Electron Microscope (SEM), dan Fourier Transform Infrared Spectroscopy (FTIR) untuk mengidentifikasi gugus fungsi gugus amina yang terdapat pada polimer. Ukuran distribusi pori pada polimer berkisar antara 1,29 sampai 3,33 µm.
摘要:步骤简单的聚合工艺已成为许多研究者关注的焦点。各种聚合物已经被开发出来,尽管一般来说,它们使用后改性方法聚合。本研究采用简单的单热法制备了季铵盐整体有机聚合物。以2-(甲基丙烯酰氧基)-N,N,N-三甲基乙胺氯为单体,二甲基丙烯酸乙酯为交联剂。在熔融石英毛细管(100 mm, 0.32 mm id x 0.45 mm od)中采用一锅法进行聚合。以异丙醇、peg400和乙醇为成孔剂,制备出完美的大孔。利用扫描电镜(SEM)对其表面形貌进行了表征,并用傅里叶变换红外光谱(FTIR)对其是否存在胺基进行了表征。聚合物的孔隙分布尺寸在1.29 ~ 3.33µm之间。摘要/ abstract摘要:本文提出了一种新的研究方法,即在不同的环境下,对不同的环境进行研究。Berbagai macam polymer telah dikembangkan, akan tetapi pada umumnya menggunakan polerisasi dengan method后修正。高分子有机,高分子有机,高分子有机,高分子有机,高分子有机,高分子有机2-(甲基丙烯酰氧基)-N,N,N-三甲基乙胺氯化钠二甲基丙烯酸乙酯单体和二甲基丙烯酸乙酯交联剂。用一锅法处理水稻(100mm, 0.32 mm长× 0.45 mm长)。Untuk mendapatkan makropori yang sempurna,异丙醇,peg400和乙醇,digunakan sebagai。扫描电子显微镜(SEM)、傅立叶变换红外光谱(FTIR)等技术研究了孟古那坎(menggunakan)聚合物。Ukuran distribubusi pori pada聚合物berkisar antara 1,29 sampai 3,33µm。
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引用次数: 1
Mineralogical, Microstructural and Compressive Strength Characterization of Fly Ash as Materials in Geopolymer Cement 粉煤灰作为地聚合物水泥材料的矿物学、微观结构和抗压强度表征
Pub Date : 2021-01-17 DOI: 10.22373/EKW.V7I1.7787
C. Rahmawati, S. Aprilia, T. Saidi, T. B. Aulia
Abstract: This study was designed to examine the mineral, microstructural, and mechanical strength properties of fly ash and its feasibility as a raw material for geopolymer cement. The study used an experimental method by examining the characteristics of fly ash by X-ray Fluorescence Spectrometer (XRF), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), hydrometer method, Scanning electron microscopy (SEM), and compressive strength testing. For creating the geopolymer cement paste, a concentration of NaOH 10M was used, with a ratio of water/solid = 0.4 and a ratio of Na2SiO3/NaOH = 1 using curring at room temperature. The results showed the geopolymer pastes have a compressive strength of 18.1 MPa and 21.5 MPa after 7 days and 28 days. The XRD results showed a decrease in the peak of 2θ at 26.54° because the amorphous part had transformed into a C-S-H solution in geopolymer cement. This finding was supported by the FTIR spectra results showing Si-O-Si bending vibration and the functional group of AlO2. It showed that Nagan Raya fly ash-based geopolymer is a potential construction material.Abstrak: Penelitian ini dirancang untuk mendapatkan sifat mineral, mikrostruktural, dan kekuatan mekanis dari fly ash serta kesesuaiannya sebagai material dasar pada semen geopolimer. Metode penelitian yang digunakan adalah metode eksperimen dengan cara  menguji karakteristik dari fly ash dengan pengujian X-ray Fluorescense Spectrometer (XRF), Fourier transform infrared (FTIR) spectoscopy, X-ray diffraction (XRD), hydrometer method, Scanning electron microscopy (SEM) dan kuat tekan.  Untuk pembuatan pasta semen geopolimer digunakan konsentrasi NaOH 10 M, rasio water/solid 0,4 dan rasio Na2SiO3/NaOH = 1 dengan perawatan pada suhu kamar. Hasil menunjukkan setelah 7 hari pasta geopolimer memiliki kuat tekan 18,1 MPa dan 21,5 MPa pada 28 hari. Hasil XRD menunjukkan adanya penurunan puncak 2θ pada 26,54° ini disebabkan karena bagian amorf dari fly ash telah menjadi larutan C-S-H pada semen geopolimer. Hasil ini diperkuat dengan analisis FTIR spectra yang menunjukkan adanya Si-O-Si bending vibration dan gugus fungsi dari AlO2. Hasil menunjukkan fly ash dari Nagan Raya potensial sebagai bahan material konstruksi berbasis geopolimer.
摘要:本研究旨在研究粉煤灰的矿物、微观结构和力学强度特性,以及粉煤灰作为地聚合物水泥原料的可行性。本研究采用实验方法,通过x射线荧光光谱仪(XRF)、傅里叶变换红外光谱(FTIR)、x射线衍射仪(XRD)、比重计法、扫描电镜(SEM)和抗压强度测试等手段对粉煤灰的特性进行了检测。为了制备地聚合物水泥浆体,使用了浓度为10M的NaOH,水固比为0.4,Na2SiO3/NaOH的比例为1,在室温下进行养护。结果表明:地聚合物膏体在7天和28天后的抗压强度分别为18.1 MPa和21.5 MPa。XRD结果表明,在26.54°处2θ峰减小,这是由于非晶态部分在地聚合物水泥中转变为C-S-H溶液所致。FTIR光谱结果显示Si-O-Si的弯曲振动和AlO2的官能团支持了这一发现。这表明纳甘拉雅粉煤灰基地聚合物是一种有潜力的建筑材料。摘要:Penelitian ini dirancang untuk mendapatkan sifat矿物,微结构,dan kekuatan mekanis dari粉煤灰serta kesuanya sebagai材料dasar paadsemen地质聚合物。x射线荧光光谱仪(XRF)、傅里叶变换红外光谱仪(FTIR)、x射线衍射仪(XRD)、比重计法、扫描电子显微镜(SEM)等。Untuk pembuatan pasta semen地理聚合物digunakan konsentrasi NaOH 10 M,水/固体0,4 dan rasio Na2SiO3/NaOH = 1 dengan perawatan pada suhu kamar。Hasil menunjukkan setelah 7 hari pasta地理聚合物memiliki kuat tekan 18,1 MPa dan 21,5 MPa paada 28 hari。Hasil XRD menunjukkan adanya penurunan puncak 2θ pada 26,54°ini disebabkan karena bagian amorf dari粉煤灰telah menjadi larutan C-S-H pada semen地质聚合物。Hasil ini diperkudengan分析FTIR光谱yang menunjukkan adanya Si-O-Si弯曲振动但gugus真菌达AlO2。哈西尔曼努朱克坎粉煤灰达里纳甘拉亚潜在的sebagai bahan材料孔武基地质聚合物。
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引用次数: 8
The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods) 鸡粪饲养罗非鱼(Oreochromis niloticus)的研究
Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.7770
O. T. Kurahman, A. Yuliawati, Lusi Haerunnisa, A. Supriyatna, T. Cahyanto, Y. Suryani, A. Supriadin, C. Hidayat, M. Masri
Abstract: In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar,  Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.
摘要:在圣训中,拉苏鲁拉圣训禁止人类食用食粪动物(宰拉拉动物)的肉和奶。在本研究中,罗非鱼被喂食鸡粪(jallallah)。然后,根据他们肠道中的微生物进行分析。本研究的目的是分离和鉴定饲喂鸡粪的鱼肠道中的细菌类型,并确定致病菌和非致病菌。以鸡粪和商品鱼食为对照,从鱼肠中分离细菌样本。通过形态鉴定、宏观和微观鉴定、生化试验等方法对细菌进行鉴定。结果表明,饲喂鸡粪的鱼肠中分离出21种细菌,其中6种为致病菌。由此可见,饲喂鸡粪的罗非鱼体内含有多种致病菌。简而言之,罗非鱼不适合食用。摘要:Dalam hais yang diriwayatkan oleh Ibnu Umar, Rasulullah SAW。Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan jallallah)。Pada penelitian ini, ikkan nila diberi pakan kotoran ayam (menjadi jallallah)。Selanjutnya就像一个简单的例子,但是分析kandungan mickroba yang terkandung didalam saluran penernaannya。Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri病原菌和非病原菌yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam。Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dankan yang diberi pakan komersil(控制)。鉴定白僵菌属,细僵菌属,微僵菌属。Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam ususikan yang diberi korotan ayam yang tergolong ke dalam属李斯特菌、葡萄球菌、Alcaligenes、Cromobacterium、Edwardisiella、微球菌、芽孢杆菌、dan Kurthia。达21个jenis terdapat 6个jenis bakteri病原菌yyitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp和Chromobacterium。[3] [1] [2] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1] [1]
{"title":"The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods)","authors":"O. T. Kurahman, A. Yuliawati, Lusi Haerunnisa, A. Supriyatna, T. Cahyanto, Y. Suryani, A. Supriadin, C. Hidayat, M. Masri","doi":"10.22373/ekw.v6i2.7770","DOIUrl":"https://doi.org/10.22373/ekw.v6i2.7770","url":null,"abstract":"Abstract: In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar,  Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78931140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Isolation and Antioxidant Activity of Phenolic Compounds from Halban Leaves (Vitex pinnata Linn) in Aceh 亚齐叶中酚类化合物的分离及抗氧化活性研究
Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.5532
M. Mastura, T. Barus, L. Marpaung, P. Simanjuntak
Abstract: An isolation and identification of phenolic compounds from Halban Leaves (Vitex pinnata Linn), a traditional medicinal plant in Aceh, has been done. Halban leaves were extracted through methanol maceration followed by isolation. The methanol extract went into n-hexane and ethyl acetate partition consecutively resulting in 3 different fractions of ethyl acetate and n-hexane extract.  The ethyl acetate fraction demonstrated the most promising antioxidant and cytotoxic activity. Therefore, purification of ethyl acetate fraction was done by column chromatography [SiO2; (i) n-hexane-ethyl acetate 10:1, 1:1 ; (ii) n-hexane-ethyl acetate = 2 : 1); (iii) n-hexane-acetone = 1:1]. The pure isolate as identified by using UV, IR, NMR, and MS spectra afforded 2 phenolic compounds, namely (1) 4-hydroxymethyl benzoate and (2) p-hydroxymethyl benzoic acid (PHBA). The antioxidant activity of compound 1 and cytotoxicity activity of 2 expressed in IC50  and LD50 was 41.08 ppm and 59.41 ppm, respectively.Abstrak: Isolasi dan identifikasi senyawa fenolik dari Daun Halban (Vite xpinnata Linn) tanaman obat tradisional asal Aceh telah dilakukan. Isolasi ini dilakukan dengan cara mengekstraksi daun halban dengan cara maserasi menggunakan pelarut metanol, Ekstrak metanol kemudian dipartiisi dengan n-heksan dan etilasetat secara berturut-turut  sehingga diperoleh  tiga  ekstrak yaitu ekstrak etil asetat, n-heksan dan air. Ekstrak etil asetat memiliki bioaktivitas sebagai antioksidan dan juga toksisitas. Pemurnian dilanjutkan dengan  cara kromatografi kolom [SiO2; (i) n-heksan-etilasetat   10:1,  1:1 ; (ii) n-heksan-etilasetat = 2 : 1); (iii) n-heksan-aseton = 1:1] dan diperoleh isolat  murni. Isolat murni tersebut diidentifikasi berdasarkan interpretasi data spektra UV, IR, NMR dan MS. Hasil interpretasi data menunjukkan daun halban (V. pinnata Linn) mengandung dua senyawa fenolik yaitu (1) 4-hidroksi metil benzoat  dan (2) asam para hidroksi benzoat (PHBA). Senyawa 1  memiliki aktivitas antioksidan dengan nilai IC50 = 41,08bpj dan senyawa 2 memiliki aktivitas toksisitas dengan nilai LC50 = 59,41.
摘要:从亚齐省传统药用植物白荆叶中分离鉴定了多酚类化合物。采用甲醇浸渍法提取哈尔板叶,然后进行分离。甲醇浸出物依次进入正己烷和乙酸乙酯分割,得到乙酸乙酯和正己烷浸出物的3个不同馏分。乙酸乙酯部分显示出最有希望的抗氧化和细胞毒活性。因此,采用柱层析[SiO2;(i)正己烷-乙酸乙酯10:1,1:1;(ii)正己烷-乙酸乙酯= 2:1);(iii)正己烷-丙酮= 1:1]。经紫外、红外、核磁共振、质谱鉴定,分离得到2种酚类化合物,分别为(1)4-羟基苯甲酸和(2)对羟基苯甲酸(PHBA)。IC50和LD50表达的化合物1的抗氧化活性和2的细胞毒活性分别为41.08 ppm和59.41 ppm。摘要/ abstract摘要:伊索西丹鉴定卡西senyawa fenolik dari Daun Halban (Vite xpinnata Linn)与传统的亚齐省telah dilakukan有关。Isolasi ini dilakukan dengan cara mengekstraksi and halban dengan cara maserasi menggunakan pelarut meol, Ekstrak meol, kemudian dipartiis dengan n-heksan, dan -heksan, dan -heksan, dan -heksan, dan -heksan, dan -heksan。研究了生物活性、抗氧化活性、抗氧化活性和抗氧化活性。perurnian dilanjutkan dengan cara kromatografi kolom [SiO2];(i) n-heksan- etilaset10:1, 1:1;(ii) n-heksan-etilasetat = 2:1);(iii) n-heksan-aseton = 1:1] Dan diperoleh分离体。用紫外光谱、红外光谱、核磁共振光谱和ms光谱分析资料,分析了紫外光、紫外光、红外光谱和ms光谱资料,分析了紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光、紫外光等。Senyawa 1型memiliki aktivitas antioksidan dengan nilai LC50 = 41,08bpj而Senyawa 2型memiliki aktivitas antioksidan dengan nilai LC50 = 59,41。
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引用次数: 2
Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens 植物病原菌土壤几丁质分解放线菌的分离与筛选
Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.7400
R. H. Wibowo, S. Sipriyadi, N. R. Mubarik, Iman Rusmana, M. Suhartono
Abstract: Chitinolytic actinobacteria are currently more widely used because of their ability as the biological control agents to the pathogenic fungi, especially in horticultural and plantation crops. This research was conducted to obtain isolates of chitinolytic soil actinobacteria from the rhizosphere of the rubber plant (Hevea brasiliensis) area in IPB University. Antifungal activities from these actinobacteria hopely able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum and Sclerotium rolfsii are used in the inhibition test of chitinolytic actinobacteria. The results successfully obtained 16 isolates of actinobacteria were grew on  Humic Acid Vitamin (HV) agar. It showed that six of 16 actinobacteria isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA). KK-15 and KK-07 isolates were able to produce the largest inhibition percentages in F. oxysporum and S. rolfsii. Based on the chitinolytic index (CI) values, KK-15 and KK-07 isolates produced CI values of 1.25 and 1.5, respectively. The morphological characteristics and Gram staining of both KK-15 and KK-07 isolates are closely relative with Streptomyces sp. Abstrak: Bakteri kitinolitik saat ini banyak diteliti karena kemampuannya sebagai agens pengendali hayati jamur patogen khususnya pada tanaman hortikultura dan perkebunan. Penelitian ini dilakukan untuk mendapatkan isolat-isolat bakteri kitinolitik asal tanah dari bagian perakaran tanaman karet (Hevea brasiliensis) di daerah perkebunan karet Institut Pertanian Bogor (IPB). Aktivitas antifungi dari bakteri ini diharapkan  mampu menghambat pertumbuhan jamur patogen tanaman secara in Vitro pada media agar-agar kitin. Fusarium oxysporum dan Sclerotium rolfsii digunakan dalam uji hambat aktinobakteri kitinolitik. Hasil penelitian memperoleh 16 isolat aktinobakteri berhasil diisolasi dengan menggunakan media agar-agar Humic Acid Vitamin (HV), dan dari 16 isolat tersebut, enam isolat mampu menghasilkan zona hambat terhadap pertumbuhan hifa dari kedua jamur patogen pada media Potato Dextrose Agar (PDA) secara in Vitro. Isolat KK-15 dan KK-07 mampu menghasilkan diameter hambatan terbesar pada jamur F. oxysporum dan S. rolfsii. Berdasarkan nilai index kitinolitik (IK), isolat KK-15 dan KK-07 menghasilkan nilai IK sebesar 1,25 dan 1,5 secara berurutan. Karakteristik morfologi dan pewarnaan Gram dari kedua isolat yaitu KK-15 dan KK-07 memiliki kedekatan dengan kelompok Streptomyces spp.
摘要/ Abstract摘要:几丁质溶解放线菌因其作为病原菌的生物防治剂而在园艺和人工林作物中得到了广泛的应用。本研究从IPB大学橡胶树区根际分离得到几丁质溶解性土壤放线菌。这些放线菌的抗真菌活性有望在体外抑制几丁质琼脂培养基上植物病原真菌的生长。采用尖孢镰刀菌和罗尔夫菌核菌对几丁质溶解放线菌进行抑菌试验。结果在腐殖酸维生素(HV)琼脂培养基上成功培养了16株放线菌。结果表明,16株放线菌中有6株对病原菌菌丝在马铃薯葡萄糖琼脂(PDA)上的生长产生抑制带。KK-15和KK-07菌株对尖孢镰刀菌和罗尔夫氏葡萄球菌的抑制率最大。根据几丁质分解指数(CI), KK-15和KK-07菌株的CI值分别为1.25和1.5。KK-15和KK-07分离株的形态特征和革兰氏染色与链霉菌属密切相关。Penelitian ini dilakukan untuk mendapatkan isolat-isolat bakteri kitinolitik asal tanah dari bagian perakaran tanaman karet(巴西树)di daerah perkebunan karet Institut Pertanian Bogor (IPB)。用琼脂-琼脂亲和蛋白进行体外抗真菌活性研究。尖孢镰刀菌和菌核镰刀菌。在离体条件下,分离得到阿克tinobakteri berhasil diisolasi denan menggunakan培养基琼脂腐殖酸维生素(HV),分离得到丹丹达16株tersebut,分离得到mampu menghasilkan zona hambat terhadap pertumbuhan hifa dari kedua jamur病原菌pada培养基马铃薯葡萄糖琼脂(PDA) secara。分离物KK-15和KK-07蒙古包,蒙古包,蒙古包,蒙古包,蒙古包,蒙古包,蒙古包,蒙古包Berdasarkan nilai index kitinolitik (IK),分离株KK-15和KK-07 menghasilkan nilai IK sebesar 1,25和1,5 secara berurutan。叶土KK-15和KK-07,叶土KK-15和叶土KK-07。
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引用次数: 1
Identification and Phylogenetic Analysis of Entomopathogenic Fungal Isolates Using Molecular Approach 昆虫病原真菌分离物的分子鉴定及系统发育分析
Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.6549
S. Syaukani, Zulia Ananda, S. Suhartono, Sirtina Sirtina, Oviana Lisa, A. Alfizar, S. Samingan
Abstract: Entomopathogenic fungi are essential to consider as one of the biological agents to control termite populations. This research aimed to molecularly identify entomopathogenic fungi isolates in termites based on the ITS rDNA region and to determine the relationship of fungi isolates. Identification was performed by DNA extraction, PCR amplification, electrophoresis, purification, and sequencing. Phylogenetic trees were generated using MEGA X.  Molecular identification showed that the ISO1 sample was Penicillium oxalicum, the ISO2 sample was Trichoderma ghanense the ISO3 sample was Aspergillus niger, the ISO4 sample was Aspergillus fumigatus and the ISO5 sample was Aspergillus pseudonomius. The phylogenetic tree showed that the ISO1, ISO2, ISO3, ISO4, and ISO5 samples had the closest relationship with Penicillium oxalicum strain FR6-CGR12, Trichoderma ghanense isolate TM2, Aspergillus niger isolate 77, Aspergillus fumigatus, and Aspergillus pseudonomius strain DTO 267D6, respectively.Abstrak: Kelimpahan jenis fungi entomopatogen adalah hal yang terpenting untuk dipertimbangkan sebagai agen hayati bagi populasi rayap.Penelitian ini bertujuan untuk mengidentifikasi secara molekular isolat fungi entomopatogen pada rayap, berdasarkan daerah ITS rDNA dan mengetahui hubungan kekerabatan dari isolat fungi tersebut. Identifikasi dilakukan dengan cara ekstraksi DNA, amplifikasi menggunakan PCR, elektroforesis, purifikasi dan sekuensing. Selanjutnya kontruksi pohon filogenetik menggunakan aplikasi MEGA X. Berdasarkan uji molekular menunjukkan bahwa sampel ISO1 merupakan Penicillium oxalicum.ISO2 merupakan Trichoderma ghanense.ISO3 merupakan Aspergillus niger. ISO4 merupakan Aspergillus fumigatus.ISO5 merupakan Aspergillus pseudonomius.Konstruksi pohon filogenetik menunjukkan bahwa, sampel ISO1 berkerabat dekat dengan Penicillium oxalicum strain FR6-CGR12. Sampel ISO2 berkerabat dekat dengan Trichoderma ghanense isolat TM2. Sampel ISO3 berkerabat dekat dengan Aspergillus nigerisolat 77. Sampel ISO4 berkerabat dekat dengan Aspergillus fumigatus.Sampel ISO5 berkerabat dekat dengan Aspergillus pseudonomius strain DTO 267D6.
摘要:昆虫病原真菌是控制白蚁种群的重要生物制剂之一。本研究旨在基于ITS rDNA区域对白蚁昆虫病原真菌分离株进行分子鉴定,并确定真菌分离株间的亲缘关系。通过DNA提取、PCR扩增、电泳、纯化和测序进行鉴定。分子鉴定表明,ISO1样品为草青霉,ISO2样品为甘木霉,ISO3样品为黑曲霉,ISO4样品为烟曲霉,ISO5样品为假曲霉。系统发育树分析结果表明,ISO1、ISO2、ISO3、ISO4和ISO5样品分别与草青霉FR6-CGR12、甘尼木霉TM2、黑曲霉77、烟曲霉和假曲霉DTO 267D6亲缘关系最密切。摘要:Kelimpahan jenis真菌昆虫病原菌adalah halyang terpenting untuk dipertimbangkan sebagai agen hayati bagi populasi rayap。分子分离真菌昆虫病原菌paadrayap, berdasarkan daerah ITS rDNA dan mengetahui hubungan kekerabatan dari isolat fungi tersebut。鉴定卡西的DNA,扩增卡西的孟古纳坎的PCR,电切,纯化卡西的丹。黄芩青霉的分子分析。黄芩青霉的分子分析。ISO2 merupakan加纳木霉。ISO3黑曲霉。ISO4烟曲霉。ISO5 merupakan伪曲霉。样品ISO1 berkerabat dekat dungan草酸青霉菌菌株FR6-CGR12。样品ISO2 berkerat dekat denengan木霉分离株TM2。样品ISO3 berkerabat dekat denengan Aspergillus nigisolat 77。样品ISO4 berkerabat dekat dengan烟曲霉。样品ISO5 berkerabat dekat dengan伪曲霉菌株DTO 267D6。
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引用次数: 0
Hotel Building Reliability Towards Building Age 面向建筑时代的酒店建筑可靠性
Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.6313
Syukri Indrawansyah, Abdullah Abdullah, C. Z. Oktaviani
Abstract: Mahara Hotel is a tourism-supporting facility and infrastructure first established in Central Aceh District in 1999. This 3-story hotel used a reinforced concrete frame structure, and as time goes by, the service capacity of the building would decrease. This condition was suspected to affect quality and user convenience. Anticipate the older age of the building, and better maintenance management is applied so that the proper building’s function can be maintained. The purpose of this study was to assess the reliability of 20 year-established hotel buildings. The method was a descriptive analysis. Data were analyzed based on the guidelines of Technical Procedures for Guidelines for Certificate of Eligibility for Functions (SLF) of Buildings Regulation of  Minister of Public Works No. 25/PRT/M/2007. Based on the results, the reliability value of the Mahara Hotel building was unreliable (66.75 %), less than the SLF guidelines (at least 75 %). Some components that caused a low level of reliability to include utility and fire protection components (20.34 %) and accessibility components (3.11 %). The effective and efficient component in increasing the reliability of the building was the utility and fire protection components by not requiring major demolition.Abstrak: Hotel Mahara merupakan sarana dan prasarana penunjang pariwisata yang pertama kali berdiri di Kabupaten Aceh Tengah pada tahun 1999. Hotel berlantai 3 ini menggunakan struktur rangka beton bertulang dan seiring dengan berjalannya waktu, daya layan gedung akan semakin menurun. Kondisi ini ditenggarai akan mempengaruhi kualitas dan kenyamanan pengguna. Untuk mengantisipasi hal tersebut, maka semakin tua umur bangunan, seharusnya didukung dengan penerapan manajemen pemeliharaan yang baik agar kelayakan fungsi dari bangunan dapat dipertahankan. Tujuan dari penelitian ini untuk menilai keandalan bangunan hotel setelah berumur 20 tahun. Metode yang digunakan adalah analisis deskriptif. Data dianalisis berdasarkan panduan Teknis Tata Cara Pedoman Sertifikat Laik Fungsi (SLF) Bangunan Gedung Peraturan Menteri Pekerjaan Umum No. 25/PRT/M/2007. Berdasarkan hasil penelitian, nilai keandalan bangunan gedung Hotel Mahara termasuk tidak andal yaitu sebesar 66.75 %, kurang dari yang dipersyaratkan oleh pedoman SLF tersebut yaitu minimal 75 %. Beberapa komponen yang menyebabkan rendahnya tingkat keandalan anatara lain komponen utilitas dan proteksi kebakaran sebesar 20.34 % serta komponen aksesibilitas sebesar 3.11 %. Komponen yang efektif serta efisien dalam menaikkan tingkat keandalan bangunan gedung tersebut adalah komponen utilitas dan proteksi kebakaran dengan tidak mengharuskan pembongkaran yang besar.
摘要:马哈拉酒店是1999年在亚齐中部地区建立的一个旅游配套设施和基础设施。这座三层的酒店采用钢筋混凝土框架结构,随着时间的推移,建筑的服务能力会下降。这种情况被怀疑会影响质量和使用方便。预计建筑物的使用年限,并采用更好的维护管理,使建筑物的适当功能得以维持。本研究的目的是评估20年历史的酒店建筑的可靠性。方法为描述性分析。数据是根据公共工程部长第25/PRT/M/2007号《建筑功能资格证书(SLF)指南技术程序》的指南进行分析的。根据结果,Mahara酒店建筑的可靠度值为不可靠(66.75%),低于SLF指南(至少75%)。导致可靠性水平较低的一些组件包括公用事业和消防组件(20.34%)和无障碍组件(3.11%)。提高建筑物可靠性的有效和高效的组成部分是公用事业和消防部分,不需要进行大规模拆除。摘要:1999年,亚齐,登加,亚齐,登加,加蓬,亚齐,登加,加蓬。伯兰泰酒店3号位于孟古纳罕,在孟古纳罕,在孟古纳罕,在孟古纳罕,在孟古纳罕,在孟古纳罕,在孟古纳罕。康迪西尼·迪登加莱,阿肯·彭加罗,我的朋友,我的朋友,我的朋友。Untuk mengantisipasi hal tersebut, maka semakin tua umur bangunan, seharusnya didukung dengan penerapan management, pemeliharaan yang baik agar kelayakan fungsi dari bangunan dapat dipertahankan。Tujuan dari penelitian ini untuk menilai keandalan bangunan hotel setelah berumur 20 tahun。Metode yang digunakan adalah分析脚本。数据分析berdasarkan panduan Teknis Tata Cara Pedoman Sertifikat Laik funsi (SLF) Bangunan Gedung Peraturan ministry Pekerjaan Umum No. 25/PRT/M/2007。Berdasarkan hasil penelitian, nilai keandalan bangunan gedung Hotel Mahara termasuk tidak andal yitu sebesar 66.75%, kurang dari yang dipersyarkan oleh pedoman SLF tersebut yitu minimal 75%。Beberapa komponen yang menyebabkan rendahnya tingkat keandalan anatara lain komponen utiliten proteksi kebakaran sebesar 20.34% komponen aksesibilitas sebesar 3.11%。Komponen yang effect kktif serta efisien dalam menaikkan tingkat kean andalan bangunan geung terung,但adalah Komponen utilitas and proteksi kebakaran dengan tidak mengharuskan pembongkaran yang besar。
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引用次数: 1
The Prevention of Ulcers Decubitus with Mobilization and The Usage of Olive Oil on Stroke Patients 运动预防脑卒中患者褥疮及橄榄油的应用
Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.6925
Siti Cahaya Meliza, Kiking Ritarwa, Nurdin Sitohang
Abstract: Decubitus ulcers are one of the problems experienced by bed rest patients, such as stroke patients, caused by immobilization. Prevention of decubitus ulcers can be done with mobilization and topical application of olive oil. Olive oil contains saturated fatty acids, unsaturated fatty acids, vitamin E, and phenols which are healthy for the skin. This research aims to identify the effects of mobilization and application of olive oil on the prevention of decubitus ulcers in stroke patients. A control group pretest-posttest quasi-experimental design was used in the research. Convenience (non-probability) sampling was also carried out, resulting in 64 research samples. Mobilization intervention of right-left oblique positions and application of olive oil on the area prone to decubitus ulcers were then executed for seven days. Data analysis using the Wilcoxon signed-rank test showed the effects of mobilization and application of olive oil on the preventions of decubitus ulcers in stroke patients with a value of P= <0,05. Abstrak: Ulkus dekubitus merupakan salah satu masalah yang dialami pasien tirah baring seperti pasien stroke, dengan faktor penyebabnya adalah immobilisasi, tindakan pencegahan ulkus dekubitus dapat dilkukan dengan mobilisasi dan pemberian bahan oles seperti minyak zaitun, minyak zaitun kandungannya terdiri dari asam lemak jenuh, asam lemak tak jenuh, vitamin E dan fenolik yang baik untuk kesehatan kulit. Penelitian ini bertujuan untuk mengidentifikasi pengaruh mobilisasi dan penggunaan minyak zaitun terhadap pencegahan ulkus dekubitus pada pasien stroke. Penelitian menggunakan desain Quasi-experimental dengan pretest- posttest with countrol gruopdesign.Convenience (Non-probability) dilakukan untuk pengambilan sampel, jumlah sampel penelitian 64. Intervensi mobilisasi miring kanan dan kiri kemudian bagian yang akan terjadi ulkus dekubitus diolesi minyak zaitun,intervensi dilakukan selama tujuh hari. Analisa data wilcoson signed -rank test menunjukan adanya pengaruh mobilisasi dan penggunaan minyak zaitun terhadap pencegahan ulkus dekubitus pada pasien stroke dangan nilai P= <0,05. Mobilisasi miring kanan dan kiri penggunaan minyak zaitun dapat mencegah ulkus dekubitus.
摘要:褥疮溃疡是卧床休息患者(如中风患者)常遇到的问题之一。预防褥疮溃疡可以做的动员和局部应用橄榄油。橄榄油含有对皮肤有益的饱和脂肪酸、不饱和脂肪酸、维生素E和酚类物质。本研究旨在确定橄榄油的动员和应用对预防中风患者褥疮溃疡的影响。研究采用对照组前测后测准实验设计。还进行了方便(非概率)抽样,共得到64个研究样本。然后进行左右斜位的活动干预,并在褥疮易发部位涂抹橄榄油,持续7天。采用Wilcoxon sign -rank检验进行数据分析,发现动员和涂抹橄榄油对脑卒中患者褥疮的预防作用,P值< 0.05。摘要:Ulkus dekubitus merupakan salah satu masalah yang dialami pasien tirah had seperti pasien卒中,dengan factor penyebabnya adalah immobilisasi, tindakan pencegahan Ulkus dekubitus dapat dilkukan denengan mobilisasi dan pemberian bahan oles seperti minyak zaitun, minyak zaitun kandungannya terdiri dari asam lemak jenuh, asam lemak tak jenuh,维生素E dan fenolik yang baik untuk kesehatan kulit。Penelitian ini bertujuan untuk mengidfikasi pengaruh mobilisasi dan penggunaan minyak zaitun terhadap penegahan ulkus dekubitus paden stroke。peneltian menggunakan设计了准实验登干前测-后测,采用对照组设计。便利(非概率)dilakukan untuk pengambilan sampel, jumlah sampel penelitian 64。干预运动是一种运动,是一种运动,是一种运动,是一种运动,是一种运动,是一种运动,是一种运动。分析数据将采用sign -rank检验menunjukan adanya pengaruh mobilisasi dan penggunaan minyak zaitun terhadap penegahan ulkus dekubitus paten卒中dangan nilai P= < 0.05。我是说,我是说,我是说,我是说,我是说,我是说,我是说。
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引用次数: 1
Knowledge Transfer About Earthquake Disaster Mitigation To Children Through TF-IDF 通过TF-IDF向儿童传递地震减灾知识
Pub Date : 2020-12-30 DOI: 10.22373/ekw.v6i2.7281
Maria Umran, H. M. Sarim
Abstract: Past observations during a disaster identify that when children are separated from parents, they suffer due to the inability to comprehend disaster mitigation concepts. This study proposes a process from the existing framework K-Nearest Neighbor (KNN) and Term Frequency - Inverse Document Frequency (TF-IDF) for extracting a large body of knowledge in the form of documents into simple words. Those simple words can be arranged into contextual lyrics utilizing an Artificial Intelligence lyrics generator and then orchestrated into a song using a music generator. The piece, which is the output of the proposed process, is utilized to transfer the knowledge about earthquake disaster mitigation to children. A quantitative analysis of questionnaires on students aged 9-10 in Banda Aceh shows the song's highly significant effect in transferring the knowledge about earthquake disaster mitigation to children.
摘要:过去在灾害期间的观察发现,当儿童与父母分离时,他们会因为无法理解减灾概念而受苦。本研究提出了一种基于k -最近邻(KNN)和Term Frequency - Inverse Document Frequency (TF-IDF)框架的过程,用于将大量文档形式的知识提取为简单的单词。这些简单的单词可以利用人工智能歌词生成器排列成上下文歌词,然后使用音乐生成器编曲成歌曲。这件作品是拟议过程的产出,用于向儿童传授有关地震减灾的知识。对班达亚齐省9-10岁学生问卷的定量分析显示,这首歌在向儿童传递地震减灾知识方面具有非常显著的效果。
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引用次数: 4
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Elkawnie
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