Electronic Journal of Biotechnology最新文献

{"title":"Transcriptomic and metabolomic profiling reveals key mechanisms of alkaline stress tolerance in rice","authors":"Jiangxu Wang ,&nbsp;Chuang Lang ,&nbsp;Yang Ren ,&nbsp;Junxiang Guo ,&nbsp;Wendong Ma ,&nbsp;Qing Liu ,&nbsp;Lei Lei ,&nbsp;Shichen Sun","doi":"10.1016/j.ejbt.2025.07.002","DOIUrl":"10.1016/j.ejbt.2025.07.002","url":null,"abstract":"<div><h3>Background</h3><div>Alkaline stress severely restricts rice growth and yield by disrupting ion balance, nutrient uptake, and oxidative metabolism. Clarifying the molecular mechanisms of tolerance is vital for breeding resilient varieties. This study explores transcriptional and metabolic adaptations in an alkali-tolerant (Qijing 10, LD) and sensitive (Tengxi 138, WL) rice variety under alkaline stress.</div></div><div><h3>Results</h3><div>Transcriptomic analysis revealed 1297 differentially expressed genes (DEGs) in the sensitive variety under alkaline stress (TWL), primarily enriched in pathways related to antioxidant enzyme synthesis (e.g., peroxidase genes), transmembrane ion transport, and membrane lipid stabilization pathways. In contrast, the tolerant variety (TLD) exhibited only 38 DEGs, suggesting transcriptional homeostasis achieved via suppression of stress-related gene overactivation. Metabolomic profiling demonstrated stable levels of key lipids (phosphatidic acid, galactolipids) and osmolytes (proline, betaine) in the tolerant variety under stress, whereas the sensitive variety accumulated lipid peroxidation products (malondialdehyde, MDA) and displayed dysregulated carbohydrate metabolic dysregulation. Integrated multi-omics analysis indicated that the tolerant variety coordinated lipid metabolism gene modulation with antioxidant metabolite accumulation, establishing dual barriers for ROS scavenging and membrane protection. Conversely, transcriptional dysregulation in the sensitive variety led to metabolic collapse.</div></div><div><h3>Conclusions</h3><div>Alkaline tolerance in rice hinges on the synergistic modulation of stress-responsive genes and metabolic networks to preserve redox equilibrium and membrane function. The tolerant variety’s capacity to stabilize transcriptional activity and metabolic flux underlies its resilience. These results elucidate key molecular and metabolic determinants of alkaline tolerance, offering strategic targets for breeding rice cultivars adapted to alkaline environments.</div><div><strong>How to cite:</strong> Wang J, Lang C, Ren Y, et al. Transcriptomic and metabolomic profiling reveals key mechanisms of alkaline stress tolerance in rice. Electron J Biotechnol 2025;78. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"78 ","pages":"Pages 35-45"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling microbial secondary metabolite biosynthetic gene clusters from alkaline soda Lake Chitu, in the Ethiopian Rift Valley","authors":"Gessesse Kebede Bekele ,&nbsp;Ermias Sissay Balcha ,&nbsp;Abu Feyisa Meka ,&nbsp;Eskedar Getachew Assefa ,&nbsp;Ebrahim M. Abda ,&nbsp;Fassil Assefa Tuji ,&nbsp;Mesfin Tafesse Gemeda","doi":"10.1016/j.ejbt.2025.06.002","DOIUrl":"10.1016/j.ejbt.2025.06.002","url":null,"abstract":"<div><h3>Background</h3><div>Microorganisms inhabiting alkalihalo-soda lakes are known for producing diverse secondary metabolites with potential biotechnological and pharmaceutical applications. This study explored the biosynthetic capabilities of microbial communities from Ethiopia’s Chitu Lake through shotgun metagenomic sequencing and metagenome-assembled genome (MAG) analyses using various bioinformatics tools.</div></div><div><h3>Results</h3><div>Analysis of MAGs using the Antibiotics and Secondary Metabolite Analysis Shell (antiSMASH) revealed 13 major types of biosynthetic gene clusters. The most abundant were terpene-precursors (32%) and terpene clusters (25%), followed by ribosomally synthesized and post-translationally modified peptides (9%) and nonribosomal peptide synthetases (7%). Other less common BGCs (5% each) included betalactone, ectoine, and Type I polyketide synthase, while rare types (2% each) comprised arylpolyene, hydrogen cyanide, phosphonate, ranthipeptide, and others. The Natural Product Domain Seeker (NaPDoS) detected ketosynthase domains linked to pharmaceutically important such as various fatty acid synthesis, modular and iterative domain classes, and condensation domain which is associated with L-amino acid coupling (LCL) domain class, such as those involved in syringomycin biosynthesis. In addition, bacteriocin analysis identified sactipeptides (56%) and lasso peptides (28%) as dominant types. Kyoto Encyclopedia of Genes and Genomes pathway analysis uncovered several secondary metabolite pathways including those for penicillin, cephalosporins, alkaloids, and phenazines. Rapid Annotation using Subsystem Technology further highlighted secondary metabolism pathways vital for microbial survival in Chitu Lake’s extreme environment.</div></div><div><h3>Conclusions</h3><div>The discovery of diverse biosynthetic gene cluster positions Chitu Lake as a valuable source of secondary metabolites, highlighting the biotechnological, industrial, pharmaceutical, agricultural and environmental potential of its extremophilic microbes and supporting further bioprospecting efforts.</div><div><strong>How to cite:</strong> Bekele GK, Balcha ES, Meka AF, et al. Unveiling microbial secondary metabolite biosynthetic gene clusters from alkaline soda Lake Chitu, in the Ethiopian Rift Valley. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.06.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 48-58"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal community dynamics and pathogenic risk in composting, vermicomposting and leachate systems amended with rabbit manure","authors":"Rocío del Pilar Serrano-Ramírez ,&nbsp;Adalberto Zenteno-Rojas ,&nbsp;Valentín Pérez-Hernández ,&nbsp;Odín Reyes-Vallejo ,&nbsp;Sugey Vásquez-Hernández ,&nbsp;Héctor Hiram Torres-Ventura ,&nbsp;Mayram Margarita González-Reyes ,&nbsp;Wilber Montejo-López ,&nbsp;Abumalé Cruz-Salomón ,&nbsp;Joaquín Adolfo Montes-Molina","doi":"10.1016/j.ejbt.2025.05.002","DOIUrl":"10.1016/j.ejbt.2025.05.002","url":null,"abstract":"<div><h3>Background</h3><div>Understanding the dynamics of fungal communities in composting and vermicomposting systems is essential for optimizing waste management practices and minimizing pathogen risks. For this reason, this study assessed the fungal community structure and potential pathogenic risks in composting, vermicomposting and leachate systems amended with rabbit manure, using Illumina’s MiSeq platform for internal transcribed spacer (ITS) sequencing and FUNGuild analysis.</div></div><div><h3>Results</h3><div>Phylotypes from Basidiomycota were predominant in all treatments, while the pathogenic genus <em>Microascus</em>, initially abundant (37%), showed a significant reduction to 5% and 7% following composting and vermicomposting, respectively, and to 1% and 13% in their leachates. Given <em>Microascus</em>’s association with human skin diseases, proper handling of organic waste is critical before its agricultural use. In contrast, the FUNGuild analysis revealed a high abundance of saprotrophic fungi such as <em>Aspergillus</em>, <em>Preussia, Botryotrichum</em>, and <em>Acremonium</em> known for producing enzymes that promote nutrient cycling and soil fertility.</div></div><div><h3>Conclusions</h3><div>These findings highlight the potential for vermicomposting to reduce pathogen risks while enhancing fungal-driven nutrient recycling, offering practical insights for sustainable agriculture and organic waste management.</div><div><strong>How to cite:</strong> Serrano-Ramírez RdP, Zenteno-Rojas A, Pérez-Hernández V, et al. Fungal community dynamics and pathogenic risk in composting, vermicomposting and leachate systems amended with rabbit manure. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 1-11"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge","authors":"Chutema Thongsongkaew ,&nbsp;Benjamas Cheirsilp ,&nbsp;Asma Billateh ,&nbsp;Wageeporn Maneechote ,&nbsp;Sirasit Srinuanpan","doi":"10.1016/j.ejbt.2025.05.005","DOIUrl":"10.1016/j.ejbt.2025.05.005","url":null,"abstract":"<div><h3>Background</h3><div>Aquaculture has become the fastest-growing sector in recent decades, and this has led to intensified cultural practices to achieve high yields. However, such practices have raised concerns regarding their environmental impact, as aquaculture sludge and wastewater cause significant organic pollution. This study then aimed to isolate and screen proteolytic, amylolytic, and lipolytic bacteria and evaluate their consortia for enzyme production and bioremediation of shrimp pond sludge.</div></div><div><h3>Results</h3><div>The strategy using multiple substrates in the isolation media successfully obtained bacterial strains with multiple hydrolytic activities. After primary and secondary quantitative screening, 18 isolates that exhibited high dual and triple hydrolytic activities were selected. After tertiary quantitative screening using synthetic shrimp pond sludge and co-culture tests, <em>Exiguobacterium indicum</em> SSP-PA-08, <em>Bacillus coagulans</em>, and <em>Bacillus subtilis</em> were selected due to their synergy for the production of triple hydrolytic enzymes. Their consortia inoculated in shrimp pond sludge containing 0.8% total suspended solids (TSSs) showed an increase in proteolytic activity by 2.5 folds and amylolytic activity by 20 folds, which led to a greater reduction in TSS. The highest enzyme production was obtained using shrimp pond sludge containing 1.6% TSS.</div></div><div><h3>Conclusions</h3><div>This study has developed the methods to isolate bacteria with multienzyme-producing ability. Co-culturing these bacteria in synthetic shrimp sludge significantly enhanced hydrolytic activity and led to a greater reduction in TSS. These strategies may contribute greatly to the hydrolytic enzyme production and environmentally friendly bioremediation of aquaculture sludge.</div><div><strong>How to cite:</strong> Thongsongkaew C, Cheirsilp B, Billateh A, et al. Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 35-47"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, purification and physicochemical characterization of Cichorium intybus L. root polysaccharide and its protective effect against CCl4-induced liver injury","authors":"Yingze Ma,&nbsp;Zhiping Yang,&nbsp;Weili Huang","doi":"10.1016/j.ejbt.2025.06.003","DOIUrl":"10.1016/j.ejbt.2025.06.003","url":null,"abstract":"<div><h3>Background</h3><div>Liver injury is a major cause of hepatic diseases, often leading to impaired liver function. Current treatments face limitations due to potential hepatotoxicity, driving interest in alternative therapies like Traditional Chinese Medicine (TCM). <em>Cichorium intybus</em> L., a medicinal herb rich in bioactive polysaccharides, has shown promise in liver protection.</div></div><div><h3>Results</h3><div>A water-soluble polysaccharide (CIP-N-1), with an average molecular weight of 2.3 kDa, was isolated and purified from the water extract of <em>Cichorium intybus</em> L. root using DEAE cellulose column chromatography and CL-6B agarose gel chromatography. CIP-N-1 consists of 98.16% neutral sugars and 1.69% proteins, with its primary components being mannose and glucose in a ratio of 4.9:95.1. In vivo studies demonstrated CIP-N-1′s hepatoprotective effects by enhancing antioxidant activity, inhibiting lipid peroxidation, and reducing inflammation in CCl₄-induced liver injury.</div></div><div><h3>Conclusions</h3><div>CIP-N-1 shows potential as a dietary supplement for alleviating chemical liver damage. Its antioxidant and anti-inflammatory properties support its use in liver health, offering a natural therapeutic option for hepatic injury prevention and treatment.</div><div><strong>How to cite:</strong> Ma Y, Yang Z, Huang W. Isolation, purification and physicochemical characterization of <em>Cichorium intybus</em> L. root polysaccharide and its protective effect against CCl4-induced liver injury. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.06.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 59-65"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144924951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Onion peel-mediated biosynthesis of TiO2-ZnO bimetallic nanoparticles: Antimicrobial, antibiofilm, and anticancer activities","authors":"Ebrahim Saied ,&nbsp;Ahmed S. Doghish ,&nbsp;Mohamed K.Y. Soliman ,&nbsp;Walaa A. El-Dakroury ,&nbsp;Abeer S. Aloufi ,&nbsp;Bushra Hafeez Kiani ,&nbsp;Amr H. Hashem","doi":"10.1016/j.ejbt.2025.06.001","DOIUrl":"10.1016/j.ejbt.2025.06.001","url":null,"abstract":"<div><h3>Background</h3><div>The rising challenges of antibiotic resistance and cancer necessitate the development of sustainable, cost-effective, and multifunctional therapeutic agents. This study introduces a green synthesis approach for bimetallic nanoparticles (BNPs) using agro-waste materials.</div></div><div><h3>Results</h3><div>For the first time, bimetallic titanium dioxide–zinc oxide (TiO₂-ZnO) BNPs were synthesized using onion peel extract as a natural reducing and stabilizing agent. UV–Vis spectroscopy confirmed nanoparticle formation with a peak corresponding to a size of approximately 320 nm. DLS showed an average hydrodynamic diameter of 145.1 nm, and TEM revealed monodispersed nanoparticles, ranging from 80 to 150 nm. The BNPs exhibited broad-spectrum antimicrobial activity with MIC values of 500 μg/mL against <em>Bacillus subtilis</em>, <em>Pseudomonas aeruginosa</em>, and <em>Candida albicans</em>; 1000 μg/mL against <em>Staphylococcus aureus</em>; and 250 μg/mL against <em>Escherichia coli</em>. They also demonstrated significant antibiofilm activity against <em>B. subtilis</em>-MRSA with a 63.1% inhibition rate at 125 μg/mL. Additionally, TiO₂-ZnO BNPs showed potent cytotoxic effects on MCF-7 breast cancer cells, with an IC₅₀ of 5.97 ± 0.37 μg/mL, and anticancer activity was mediated by caspase-8 activation and VEGFR-2 downregulation.</div></div><div><h3>Conclusions</h3><div>This green-synthesized TiO₂-ZnO BNPs offer a promising dual-function nanoplatform for combating microbial infections and cancer, highlighting the potential of sustainable nanotechnology for biomedical applications.</div><div><strong>How to cite:</strong> Saied E, Doghish AS, Soliman MK, et al. Onion peel-mediated biosynthesis of TiO₂-ZnO bimetallic nanoparticles: Antimicrobial, antibiofilm, and anticancer activities. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.06.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 12-23"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NEDD4L/RHOF axis suppresses the malignant phenotypes and lipid metabolism of breast cancer and NEDD4L is affected by upstream ALKBH5","authors":"Tao Liu,&nbsp;Xiaoming Lin,&nbsp;Rong Liang","doi":"10.1016/j.ejbt.2025.07.001","DOIUrl":"10.1016/j.ejbt.2025.07.001","url":null,"abstract":"<div><h3>Background</h3><div>The protein posttranslational modifications, including ubiquitination and methylation, exhibit the essential function in breast cancer. Herein, we aimed to explore the molecular mechanism of neural precursor cell-expressed developmentally downregulated gene 4-like (NEDD4L) associated with Rho GTPase Rif (RHOF) and AlkB homolog 5 (ALKBH5). A series of experiments including expression detection, cell functions, xenograft tumor assay, and interaction analysis was designed.</div></div><div><h3>Results</h3><div>RHOF was up-regulated in breast cancer samples and cells. Silencing RHOF suppressed breast cancer cell growth, migration, invasion and lipid metabolism. Breast cancer tumorigenesis and lipid metabolism were repressed by RHOF knockdown <em>in vivo.</em> NEDD4L impaired RHOF stability by promoting its ubiquitination. NEDD4L overexpression restrained breast cancer cell progression and lipid metabolism via degrading RHOF. ALKBH5 inhibited NEDD4L expression through m6A modification.</div></div><div><h3>Conclusions</h3><div>These results evidenced that NEDD4L facilitated the malignant progression of breast cancer via inducing the ubiquitination of RHOF, and NEDD4L was also affected by ALKBH5-mediated m6A demethylation.</div><div><strong>How to cite:</strong> Liu T, Lin X, Liang R. NEDD4L/RHOF axis suppresses the malignant phenotypes and lipid metabolism of breast cancer and NEDD4L is affected by upstream ALKBH5. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.07.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 66-79"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved resistance to basal rot disease and promotion of onion plant growth by Aspergillus terreus-mediated silver nanoparticles","authors":"Mohamed G.A. Hegazy ,&nbsp;Mahmoud Gad ,&nbsp;Sobhi F. Lamlom ,&nbsp;Islam I. Teiba ,&nbsp;Osama A.M. Al-Bedak ,&nbsp;Muhammad Moaaz Ali ,&nbsp;Eman Alhomaidi ,&nbsp;Mona Saleh Al Tami ,&nbsp;Ahmed F. Yousef ,&nbsp;Waleed M. Ali","doi":"10.1016/j.ejbt.2025.05.003","DOIUrl":"10.1016/j.ejbt.2025.05.003","url":null,"abstract":"<div><h3>Background</h3><div>Onions, a vital agricultural crop rich in carbohydrates and essential minerals, face severe threats from <em>Fusarium oxysporum</em>, the causative agent of basal rot disease. This study assessed the effectiveness of extracellular, green-synthesized silver nanoparticles (AgNPs), produced by <em>Aspergillus terreus</em> AUMC 15760, in managing basal rot disease caused by <em>F. oxysporum</em> AUMC 15798.</div></div><div><h3>Results</h3><div>AgNPs ranging from 12.1 to 28.7 nm were incorporated into PDA media at concentrations of 50, 100, 150, and 200 ppm, achieving fungal growth inhibition rates of 24.88%, 40.77%, 54.44%, and 69.33%, respectively. A greenhouse experiment was carried out using onion seedlings, with a randomized complete block design (RCBD) to compare eight different treatments: 100 ppm AgNPs (spray), 50 ppm AgNPs (spray), 100 ppm AgNPs (soil application), 50 ppm AgNPs (soil application), Dovex spray (50%), Dovex soil application (50%), a negative control, and a positive control. Greenhouse results showed a significant reduction in disease severity, with Dovex lowering it to 20%. AgNPs at 50 ppm reduced severity to 57.77% (soil) and 35.55% (spray), while 100 ppm further decreased it to 31.1% (soil) and 22.2% (spray). The application of 100 ppm AgNPs improved plant growth parameters. It also enhanced chlorophyll <em>a</em>, chlorophyll <em>b</em>, and carotenoid levels. The greatest reductions in phenolic (0.34 mg/g) and anthocyanin contents (0.48 mg/g), as well as peroxidase (0.44 µmol/min) and catalase activities (0.19 µmol/min), were recorded in plants treated with 100 ppm AgNPs (spray).</div></div><div><h3>Conclusions</h3><div>AgNPs effectively control basal rot disease, boost plant growth, and regulate antioxidant activity.</div><div><strong>How to cite:</strong> Hegazy MG, Gad M, Lamlom SF, et al. Improved resistance to basal rot disease and promotion of onion plant growth by <em>Aspergillus terreus</em>-mediated silver nanoparticles. Electron J Biotechnol 2025;77. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"77 ","pages":"Pages 24-34"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of flaxseed’s supplement on AMPKα2′s expression in non-diabetic and diabetic patients having follicular thyroid carcinoma on mitochondrial’s ATP synthase for TPC-1 cell line","authors":"Amena Alsakran ,&nbsp;Afaf Aldahish ,&nbsp;Amani Alotaibi ,&nbsp;Dalal Alshaya ,&nbsp;Elham Alzahrani ,&nbsp;Mohammad Alshugeer ,&nbsp;Manal Alduwish ,&nbsp;Dalia Domiaty ,&nbsp;Fatimah Albani ,&nbsp;Wedad Al-Qahtani","doi":"10.1016/j.ejbt.2025.03.006","DOIUrl":"10.1016/j.ejbt.2025.03.006","url":null,"abstract":"<div><h3>Background</h3><div>This study examined the effects of flaxseed oil supplementation on the expression of adenosine monophosphate-activated protein kinase alpha2 (AMPKα2) in male patients with follicular thyroid cancer. The objective was to assess whether flaxseed oil could enhance AMPKα2 activity, improve histological features, and reduce tumor cell viability, offering a potential therapeutic benefit for diabetic and non-diabetic patients.</div></div><div><h3>Results</h3><div>A total of 303 patients, including 149 non-diabetic and 154 diabetic participants, received 1250 mg of flaxseed oil daily for one month. Flaxseed oil supplementation significantly improved tumor histology, with treated tissues showing organized follicular patterns and reduced fibrovascular cells compared to untreated tissues. Cell viability assays demonstrated a dose-dependent reduction in papillary thyroid carcinoma cell viability, with the strongest effects observed at flaxseed extract concentrations of 80, 50, and 30 µg/mL. Molecular analyses revealed increased AMPKα2 expression in both treated thyroid cancer tissues and cell line models, indicating enhanced protein activation. These findings suggest that flaxseed oil supplementation may inhibit tumor proliferation and improve histological organization in follicular thyroid cancer.</div></div><div><h3>Conclusions</h3><div>Flaxseed oil supplementation activates AMPKα2, reduces tumor cell viability, and improves histopathological features in follicular thyroid cancer tissues. These results highlight the potential of flaxseed oil as an adjunctive therapy to enhance cancer management strategies, particularly in diabetic and non-diabetic patients with follicular thyroid cancer.</div><div><strong>How to cite:</strong> Alsakran A, Aldahish A, Alotaibi A, et al. Effect of flaxseed’s supplement on AMPKα2’s expression in non-diabetic and diabetic patients having follicular thyroid carcinoma on mitochondrial’s ATP synthase for TPC-1 cell line. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 57-66"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biological agent rituximab ameliorates DNA damage and repair efficiency in the somatic cells of arthritic mice","authors":"Mohammed A. Al-Hamamah ,&nbsp;Mohamed A. Mahmoud ,&nbsp;Moureq R. Alotaibi ,&nbsp;Ahmed Nadeem ,&nbsp;Mushtaq A. Ansari ,&nbsp;Sheikh F. Ahmad ,&nbsp;Saleh A. Bakheet ,&nbsp;Gamaleldin I. Harisa ,&nbsp;Sabry M. Attia","doi":"10.1016/j.ejbt.2025.03.004","DOIUrl":"10.1016/j.ejbt.2025.03.004","url":null,"abstract":"<div><h3>Background</h3><div>Rheumatoid arthritis (RA) is an autoimmune disorder that deteriorates joints and can affect various physiological systems if not properly managed. The persistent joint inflammation and oxidative damage caused by RA can lead to genomic instability, a primary feature of most cancer cells. In RA, the prevalence of malignancy is comparatively higher than in the general population. However, it is unclear if the disease itself or its treatments induce susceptibility to neoplastic disorders. Our goal was to study genomic instability in the arthritic mouse model, namely DNA damage and repair and determine if the long-term use of the biological agent rituximab can impact these changes.</div></div><div><h3>Results</h3><div>Our results show that rituximab did not disrupt genomic stability at the tested regimen. Arthritic mice had more spontaneous DNA damage and a slower repair rate than control mice. Redox imbalance, oxidative DNA damage, and disturbance in the DNA repair pathways are also increased in arthritic mice. Meanwhile, rituximab treatment reduced oxidative DNA damage and accelerated DNA repair rate, improving redox balance and restoring the disturbances in the DNA repair gene expression at both mRNA as determined by RT² Profiler PCR array and protein levels as determined by Western blotting analysis in arthritic animals. Additionally, the ameliorative effect of rituximab has also been shown by its ability to reduce the severity of joint inflammation and histopathological alterations induced by arthritis.</div></div><div><h3>Conclusions</h3><div>The findings indicate that rituximab is a non-genotoxic, safe, effective drug for the treatment of RA and its complications.</div><div><strong>How to cite:</strong> Al-Hamamah MA, Mahmoud MA, Alotaibi MR, et al. The biological agent rituximab ameliorates DNA damage and repair efficiency in the somatic cells of arthritic mice. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 67-78"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}