Electronic Journal of Biotechnology最新文献_第2页

Anoikis- and m6A-related lncRNA analysis to identify prognostic indicators in liver hepatocellular carcinoma Anoikis-和m6a相关lncRNA分析鉴定肝癌预后指标

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2026-01-01 Epub Date: 2025-12-05 DOI: 10.1016/j.ejbt.2025.100701

Pan Yu , Shuaiyang Jing , Sarinder Kaur Dhillon

Background

In cancer, the process of anoikis is intimately associated with the emergence and progression. N6-methyladenosine modification and m6A modification play an important role in regulating long non-coding RNAs. The liver hepatocellular carcinoma patients’ data, including clinical and prognostic data, were obtained via The Cancer Genome Atlas database. The univariate, multivariate Cox and Least Absolute Selection Operator (LASSO) regression were performed to gain anoikis- and m6A-related lncRNAs. The Kaplan-Meier method was employed to assess the overall survival rate for groups of high- and low risks.

Results

A signature comprising six anoikis- and m6A-related lncRNAs was constructed: AL117336.3, LINC01138, Z83851.1, NRAV, CASC19 and AC009283.1. The clinicopathological variables, the anoikis- and m6A-related lncRNA signature demonstrated superior diagnostic efficacy, with an area under the receiver operating characteristic curve of 0.810. In the high-risk group, the overall survival was shown to be inferior to that of in group of low risk, while patients were classified by distinct clinicopathological variables. The ssGSEA and CIBERSORT immune analysis demonstrated that the predictive signature was significantly associated with liver cancer patients’ immune status. The chemotherapy drugs ATRA, AUY922, bexarotene, gemcitabine, mitomycin-C, and PHA have been found to have greater sensitivity in treating high-risk patients. qRT-PCR showed that Z83851.1, NRAV and CASC19 lncRNAs were associated with poor prognosis and were high-risk factors. AC009283.1 lncRNA may have anti-cancer properties.

Conclusions

The predictive signature is capable of independently predicting the prognosis of liver cancer patients for understanding the mechanisms of anoikis- and m6A-related lncRNAs in liver hepatocellular carcinoma and offering clinical guidance to patients with liver cancer.

How to cite: Yu P, Jing S, Dhillon SK. Anoikis and m6A related lncRNAs analysis to identify prognostic indicators in liver hepatocellular carcinoma. Electron J Biotechnol 2026;79. https://doi.org/10.1016/j.ejbt.2025.100701.

在癌症中，肿瘤的发生与发展密切相关。n6 -甲基腺苷修饰和m6A修饰在长链非编码rna调控中发挥重要作用。通过The Cancer Genome Atlas数据库获取肝细胞癌患者的临床和预后数据。采用单因素、多因素Cox和最小绝对选择算子（LASSO）回归获得anoikis和m6a相关的lncrna。Kaplan-Meier法用于评估高、低风险组的总生存率。结果构建了由6个anoikis和m6a相关lncrna组成的特征图谱：AL117336.3、LINC01138、Z83851.1、NRAV、CASC19和AC009283.1。临床病理变量anoikis-和m6a相关的lncRNA特征表现出较好的诊断效能，其在受试者工作特征曲线下的面积为0.810。高危组患者的总生存率明显低于低危组，而患者的临床病理指标不同。ssGSEA和CIBERSORT免疫分析表明，预测特征与肝癌患者的免疫状态显著相关。化疗药物ATRA、AUY922、贝沙罗汀、吉西他滨、丝裂霉素- c、PHA在治疗高危患者中具有较高的敏感性。qRT-PCR显示Z83851.1、NRAV和CASC19 lncrna与预后不良相关，为高危因素。AC009283.1 lncRNA可能具有抗癌特性。结论该预测特征能够独立预测肝癌患者的预后，有助于了解anoikis-和m6a相关lncrna在肝癌中的作用机制，为肝癌患者提供临床指导。Yu P, Jing S, Dhillon SK. Anoikis和m6A相关lncRNAs分析在肝细胞癌中的预后指标。中国生物医学工程学报（英文版）；2009；https://doi.org/10.1016/j.ejbt.2025.100701。

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引用次数: 0

Mapping the genomic position of xylanase genes on Bacillus safensis FB03 and optimizing the xylanase fermentation medium by Box-Behnken Design from an unconventional carbon source 基于Box-Behnken设计的非常规碳源木聚糖酶发酵培养基优化及木聚糖酶基因定位研究

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-07-31 DOI: 10.1016/j.ejbt.2025.05.004

Farhana Boby , Md Nurul Huda Bhuiyan , Md Mashud Parvez , Md Jahidul Islam , Ifrat Jannati

Background

The ability of Bacillus safensis to synthesize xylanase and other industrially important enzymes utilizing lignocellulosic biomass makes it advantageous for a variety of biotechnology applications. Thus, the current investigation aimed to optimize conditions and medium components for maximizing xylanase production by a newly isolated Bacillus safensis strain using banana rachis (peel of banana tree) as a novel source of carbon.

Result

Upon employing Box-Behnken Design (BBD) statistical approach, the highest enzyme activity was obtained 25.24 U/ml at 2 g/L banana rachis, 1 g/L yeast extract, 1 g/L K₂HPO₄, 5 g/L NaNO₃, 35°C and 72 h of incubation time. The purified enzyme showed 10 times higher enzyme activity (143.6 U/ml) with 2.3 mg/ml protein concentration. The enzyme was found to maintain stability up to 60°C in a wide range of pH (6 to 10). Analysis of whole genome sequencing data revealed the presence of xylanase production and xylan metabolic genes (xynA, xynB, xylP, xylT) on Bacillus safensis FB03. Also, from genome annotation, different carbohydrate metabolic genes such as glycoside hydrolases (GHs), glycosyl transferases (GTs), polysaccharide lyases (PLs), carbohydrate esterases (CEs), auxiliary activities (AAs), and carbohydrate binding modules (CBMs) were identified.

Conclusions

In accordance with our research, banana rachis can be considered as a major medium component to develop an economical fermentation process for the production of xylanase by Bacillus safensis FB03. Additionally, identification of the genomic location of xyl genes provides valuable insight towards genetic engineering for the development of a more potent industrial strain.

How to cite: Boby F, Huda Bhuiyan MN, Parvez MM, et al. Mapping the genomic position of xylanase genes on Bacillus safensis FB03 and optimizing the xylanase fermentation medium by Box-Behnken Design from an unconventional carbon source. Electron J Biotechnol 2025;78. https://doi.org/10.1016/j.ejbt.2025.05.004.

萨法芽孢杆菌利用木质纤维素生物质合成木聚糖酶和其他工业上重要的酶的能力使其在各种生物技术应用中具有优势。因此，本研究旨在优化新分离的萨菲芽孢杆菌菌株的条件和培养基成分，以香蕉轴（香蕉树的皮）作为新的碳源，最大限度地提高木聚糖酶的产量。结果采用Box-Behnken设计（BBD）统计方法，在香蕉茎2 g/L、酵母浸膏1 g/L、K2HPO4 1 g/L、NaNO3 5 g/L、35℃、72 h孵育条件下，酶活性最高为25.24 U/ml。当蛋白浓度为2.3 mg/ml时，酶活性提高10倍（143.6 U/ml）。发现该酶在60°C内保持稳定，pH值范围很广（6至10）。全基因组测序数据分析显示，萨福芽孢杆菌FB03存在木聚糖酶生产和木聚糖代谢基因（xynA, xynB, xylP, xylT）。此外，通过基因组注释，鉴定了不同的碳水化合物代谢基因，如糖苷水解酶（GHs）、糖基转移酶（GTs）、多糖裂解酶（PLs）、碳水化合物酯酶（CEs）、辅助活性（AAs）和碳水化合物结合模块（CBMs）。结论根据本研究结果，香蕉茎可作为萨菲芽孢杆菌FB03经济型发酵生产木聚糖酶的主要培养基成分。此外，鉴定xyl基因的基因组位置为开发更有效的工业菌株的基因工程提供了有价值的见解。引用方式：Boby F， Huda Bhuiyan MN， Parvez MM等。基于Box-Behnken设计的非常规碳源木聚糖酶发酵培养基优化及木聚糖酶基因定位研究中国生物医学工程学报（英文版）；2009;38。https://doi.org/10.1016/j.ejbt.2025.05.004。

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引用次数: 0

ALYREF promotes malignant behaviors and inhibits ferroptosis in colon cancer cells by stabilizing PCSK9 mRNA ALYREF通过稳定PCSK9 mRNA促进结肠癌细胞的恶性行为并抑制铁下垂

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-09-16 DOI: 10.1016/j.ejbt.2025.07.003

Lili Cao , Ying Chen , Jing Yu , Dian Yin

Background

Colon cancer is a prevalent malignancy causing significant global morbidity and mortality. The RNA methyltransferase Aly/REF export factor (ALYREF), which binds 5-methylcytosine (m5C)-modified messenger RNA, represents a potential diagnostic and therapeutic target in cancer. However, its specific role and mechanism in colon cancer progression remain unexplored.

Results

ALYREF expression was significantly elevated in colon cancer tissues and cell lines compared to normal controls. Depletion of ALYREF suppressed colon cancer cell proliferation, migration, and invasion, while simultaneously promoting apoptosis and ferroptosis. Analysis revealed proprotein convertase subtilisin/kexin type 9 (PCSK9) is highly expressed in colon cancer and positively regulated by ALYREF. Mechanistically, ALYREF directly bound to and stabilized PCSK9 messenger RNA in a manner dependent on m5C modification. Crucially, the anti-tumor effects resulting from ALYREF knockdown were reversed by overexpressing PCSK9. Consistent with cellular findings, silencing ALYREF significantly inhibited tumor growth in vivo using xenograft models.

Conclusions

This study demonstrates that ALYREF drives colon cancer malignancy by stabilizing PCSK9 messenger RNA via m5C methylation, thereby enhancing PCSK9 expression. These findings establish the ALYREF/PCSK9 axis as a critical mechanism in colon cancer progression, highlighting its potential as a novel therapeutic target for intervention.

How to cite: Cao L, Chen Y, Yu J, et al. ALYREF promotes malignant behaviors and inhibits ferroptosis in colon cancer cells by stabilizing PCSK9 mRNA. Electron J Biotechnol 2025;78. https://doi.org/10.1016/j.ejbt.2025.07.003.

背景：结肠癌是一种普遍存在的恶性肿瘤，在全球范围内具有很高的发病率和死亡率。RNA甲基转移酶Aly/REF输出因子（ALYREF）结合5-甲基胞嘧啶（m5C）修饰的信使RNA，是癌症诊断和治疗的潜在靶点。然而，其在结肠癌进展中的具体作用和机制尚不清楚。结果salyref在结肠癌组织和细胞系中的表达明显高于正常对照组。ALYREF的缺失抑制结肠癌细胞的增殖、迁移和侵袭，同时促进细胞凋亡和铁下垂。分析显示，蛋白转化酶subtilisin/ keexin type 9 （PCSK9）在结肠癌中高表达，并受到ALYREF的正调控。在机制上，ALYREF以依赖于m5C修饰的方式直接结合并稳定PCSK9信使RNA。至关重要的是，ALYREF敲低导致的抗肿瘤作用通过过表达PCSK9被逆转。与细胞研究结果一致，在异种移植模型中，沉默ALYREF显著抑制肿瘤生长。结论本研究表明，ALYREF通过m5C甲基化稳定PCSK9信使RNA，从而提高PCSK9的表达，从而驱动结肠癌恶性肿瘤。这些发现确立了ALYREF/PCSK9轴在结肠癌进展中的关键机制，突出了其作为新的干预治疗靶点的潜力。引用方式：曹磊，陈毅，余杰，等。ALYREF通过稳定PCSK9 mRNA促进结肠癌细胞的恶性行为并抑制铁下垂。中国生物医学工程学报（英文版）；2009;38。https://doi.org/10.1016/j.ejbt.2025.07.003。

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引用次数: 0

Qualitative phytochemical analysis, thin-layer chromatographic profiling, and antimicrobial potential of banana cultivars 香蕉品种的定性植物化学分析、薄层色谱分析及抗菌潜力

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-09-20 DOI: 10.1016/j.ejbt.2025.07.005

Ajmal Khan , Rony Swennen , Sujogya Kumar Panda , Liliane Schoofs , Walter Luyten

Background

Banana plants possess numerous medicinal properties due to the presence of various phytochemicals. This study aimed to assess the phytochemical profile of the crude extracts of leaf, pseudostem, and corm parts of selected banana cultivars via standard techniques and thin-layer chromatography (TLC) and to evaluate their antimicrobial activities against several food-borne and clinically important human pathogens, including two Gram-positive bacteria, six Gram-negative bacteria, and four yeasts.

Results

The results demonstrated that the Cachaco (41 %), Tereza (38 %), Fougamou (30 %), Pelipita (28 %), Giant Cavendish (26 %), and Kluai Teparot (26 %) cultivars presented significant antimicrobial activity against pathogens compared with Dole (24 %), Namwah Khom (20 %), and Mbwazirume (16 %) cultivars. Moreover, the leaves (40 %) of cultivars extracted in water (61 %) and acetone (55 %) yielded the most active antimicrobial extracts compared with the pseudostem (33 %) and corm (26 %) extracts prepared in ethanol (38 %) or hexane (28 %). Overall, the antimicrobial activities with the lowest 50 % inhibitory concentration (IC₅₀) values, especially those with values less than 200 µg/mL for bacteria and 100 µg/mL for yeasts, were reported in the leaves of Cachaco and Giant Cavendish, followed by different parts of Tereza, Pelipita, and other banana cultivars. Phytochemical analysis and TLC profiling confirmed the presence of various groups of phytochemicals in the extracts of the selected banana cultivars.

Conclusions

This study revealed that the Cachaco, Giant Cavendish, Pelipita, and Tereza cultivars possess significant antimicrobial activity, warranting further bioassay-guided antimicrobial studies for the isolation and identification of bioactive compounds, which could be useful as novel drug candidates with the highest potency.

How to cite: Khan A, Swennen R, Panda SK, et al. Qualitative phytochemical analysis, thin-layer chromatographic profiling, and antimicrobial potential of banana cultivars. Electron J Biotechnol 2025;78. https://doi.org/10.1016/j.ejbt.2025.07.005.

香蕉植物由于含有多种植物化学物质而具有许多药用特性。本研究旨在通过标准技术和薄层色谱法（TLC）评估选定香蕉品种叶片、假茎和球茎部分粗提物的植物化学特征，并评估其对几种食源性和临床重要的人类病原体（包括2种革兰氏阳性菌、6种革兰氏阴性菌和4种酵母）的抗菌活性。结果结果表明，与Dole（24%）、Namwah Khom（20%）和Mbwazirume（16%）品种相比，Cachaco（41%）、Tereza（38%）、Fougamou（30%）、Pelipita（28%）、Giant Cavendish（26%）和Kluai Teparot（26%）品种的抑菌活性显著。此外，与假茎（33%）和球茎（26%）在乙醇（38%）或己烷（28%）中提取的提取物相比，在水（61%）和丙酮（55%）中提取的品种叶片（40%）产生了最有效的抗菌提取物。总体而言，50%抑制浓度（IC50）最低的抗菌活性是在Cachaco和Giant Cavendish的叶片中，特别是细菌和酵母的IC50值小于200µg/mL，其次是Tereza、Pelipita和其他香蕉品种的不同部位。植物化学分析和薄层色谱分析证实了所选香蕉品种提取物中存在各种类型的植物化学物质。结论该研究表明，Cachaco、Giant Cavendish、Pelipita和Tereza具有显著的抗菌活性，值得进一步进行生物测定指导下的抗菌研究，以分离和鉴定生物活性化合物，从而开发出高效的新型候选药物。引用方式：Khan A， Swennen R， Panda SK等。香蕉品种的定性植物化学分析、薄层色谱分析及抗菌潜力。中国生物医学工程学报（英文版）；2009;38。https://doi.org/10.1016/j.ejbt.2025.07.005。

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引用次数: 0

Whole-genome analysis and biosynthetic gene cluster profiling of Stenotrophomonas sp. ASucR1 isolated from Sof Umer Cave, Ethiopia 埃塞俄比亚sofumer Cave窄养单胞菌ASucR1的全基因组分析及生物合成基因聚类分析

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-09-16 DOI: 10.1016/j.ejbt.2025.07.004

Abu Feyisa Meka , Gessesse Kebede Bekele , Selfu Girma Gebre , Musin Kelel Abas , Mesfin Tafesse Gemeda

Background

Sof Umer Cave is a unique habitat that hosts industrially significant microbes. In this study, Stenotrophomonas sp. ASucR1 was isolated from the cave rock and screened for antimicrobial activity. High-molecular-weight genomic DNA was extracted and subjected to whole-genome sequencing using the Illumina NovaSeq platform. Comprehensive genomic and biosynthetic gene cluster (BGC) profiling was conducted.

Results

In vitro tests revealed that Stenotrophomonas sp. ASucR1 exhibited a broad spectrum of antagonistic activity. Functional genome annotation identified diverse biosynthetic gene clusters (BGCs) and metabolic pathways, including genes involved in the synthesis of secondary metabolites. A total of 19 BGCs were identified, several of which showed no matches in the minimum information about a biosynthetic gene cluster (MiBIG) database, indicating the presence of previously uncharacterized bioactive compounds. Single-nucleotide polymorphism (SNP) analysis showed that 91.5% of variants were identified within coding regions, with 85.84% being synonymous. Classification of SNPs and insertion-deletion mutations through clusters of orthologous groups (COG) analysis highlighted their association with key biological functions.

Conclusions

This study highlights the metabolic versatility and biosynthetic potential of Stenotrophomonas sp. ASucR1, a promising candidate for antimicrobial development and biotechnological applications. The identification of various biosynthetic gene clusters paves the way for exploring bioactive compounds with pharmaceutical significance.

How to cite: Meka AF, Bekele GK, Gebre SG, et al. Whole genome analysis and biosynthetic gene cluster profiling of Stenotrophomonas sp. ASucR1 isolated from Sof Umer Cave, Ethiopia. Electron J Biotechnol 2025;78. https://doi.org/10.1016/j.ejbt.2025.07.004.

Umer洞穴是一个独特的栖息地，拥有工业上重要的微生物。本研究从洞穴岩中分离到窄养单胞菌ASucR1，并对其抗菌活性进行了筛选。提取高分子量基因组DNA，使用Illumina NovaSeq平台进行全基因组测序。进行了全面的基因组和生物合成基因簇（BGC）分析。结果体外实验表明，窄养单胞菌ASucR1具有广谱拮抗活性。功能基因组注释鉴定了多种生物合成基因簇（BGCs）和代谢途径，包括参与次级代谢物合成的基因。共鉴定出19个bgc，其中一些在生物合成基因簇（MiBIG）数据库的最小信息中没有匹配，表明存在以前未表征的生物活性化合物。单核苷酸多态性（SNP）分析显示，91.5%的变异在编码区，85.84%的变异是同义的。通过同源群（COG）分析对snp和插入-删除突变进行分类，突出了它们与关键生物学功能的关联。结论该研究突出了窄养单胞菌ASucR1的代谢多样性和生物合成潜力，是一种具有抗菌开发和生物技术应用前景的候选细菌。各种生物合成基因簇的鉴定为探索具有药用意义的生物活性化合物铺平了道路。引用方式：Meka AF， Bekele GK， Gebre SG等。埃塞俄比亚sofumer Cave窄养单胞菌ASucR1的全基因组分析及生物合成基因聚类分析中国生物医学工程学报（英文版）；2009;38。https://doi.org/10.1016/j.ejbt.2025.07.004。

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引用次数: 0

Tianma granules: Bridging traditional medicine and modern science to combat colorectal cancer via ferroptosis 天麻颗粒：连接传统医学与现代科学，通过铁下垂对抗结直肠癌

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-08-23 DOI: 10.1016/j.ejbt.2025.06.004

Ning Ding , Xiaojuan Tang , Yijing Zhang , Hongbiao Luo , Yanbo Tang , Chaoqun Zeng , Yongheng He , Liang Zhao

Background

This study aims to investigate the ferroptosis-inducing effects of Tianma Granules (TMGs) in colorectal cancer and elucidate its molecular mechanisms. Ferroptosis, an iron-dependent form of regulated cell death, represents a novel therapeutic target for cancer. We combined network pharmacology with experimental validation to explore TMG’s anti-cancer potential through ferroptosis modulation.

Results

Network pharmacology identified 382 ferroptosis-related genes overlapping with 12,944 CRC-associated targets (p < 0.05), with SLC7A11, GPX4, SAT1, PTGS2, and GLS2 prioritized as core targets. In vitro, TMG dose-dependently suppressed CRC cell proliferation (p < 0.05), elevated reactive oxygen species (p < 0.05) and ferrous ion levels (p < 0.01), effects reversed by ferroptosis inhibitor, Ferrostatin-1. c-Casp3 levels were unchanged (p > 0.05), excluding apoptosis. Transmission electron microscopy revealed mitochondrial cristae fragmentation and vacuolation, hallmark features of ferroptosis. Molecular analyses demonstrated TMG-mediated downregulation of SLC7A11 and GPX4, alongside upregulation of SAT1, PTGS2, and GLS2 (p < 0.05). In xenograft models, high-dose TMG (23.2 g/kg) reduced tumor volume, attenuated cachexia, and elevated intratumoral ROS and Fe²⁺ levels (p < 0.01), corroborating ferroptosis induction in vivo.

Conclusions

TMG suppresses CRC progression by inducing ferroptosis via dual inhibition of SLC7A11/GPX4 and activation of SAT1/PTGS2/GLS2. This study bridges traditional medicine and ferroptosis biology, positioning TMG as a novel therapeutic candidate for CRC.

How to cite: Ding N, Tang X, Zhang Y, et al. Tianma granules: Bridging traditional medicine and modern science to combat colorectal cancer via ferroptosis. Electron J Biotechnol 2025;78. https://doi.org/10.1016/j.ejbt.2025.06.004.

本研究旨在探讨天麻颗粒在结直肠癌中的诱导铁凋亡作用，并阐明其分子机制。铁下垂是一种铁依赖性的细胞死亡形式，是癌症治疗的新靶点。我们将网络药理学与实验验证相结合，探索TMG通过调节铁下垂的抗癌潜力。结果网络药理学鉴定出382个铁中毒相关基因与12944个crc相关靶点重叠（p < 0.05），其中SLC7A11、GPX4、SAT1、PTGS2和GLS2优先作为核心靶点。在体外，TMG剂量依赖性地抑制结直肠癌细胞增殖（p < 0.05），提高活性氧（p < 0.05）和亚铁离子水平（p < 0.01），这种作用被铁下垂抑制剂铁抑素-1逆转。c-Casp3水平不变（p > 0.05），不包括细胞凋亡。透射电镜显示线粒体嵴碎裂和空泡化，这是铁下垂的标志特征。分子分析显示，tmg介导SLC7A11和GPX4下调，SAT1、PTGS2和GLS2上调（p < 0.05）。在异种移植模型中，高剂量TMG （23.2 g/kg）减少了肿瘤体积，减轻了恶病质，升高了肿瘤内ROS和Fe2+水平（p < 0.01），证实了铁下垂在体内的诱导作用。结论stmg通过双重抑制SLC7A11/GPX4和激活SAT1/PTGS2/GLS2来诱导铁下垂，从而抑制结直肠癌的进展。这项研究将传统医学和铁下垂生物学联系起来，将TMG定位为CRC的一种新的治疗候选药物。引用方式：丁宁，唐旭，张勇，等。天麻颗粒：连接传统医学与现代科学，通过铁下垂对抗结直肠癌。中国生物医学工程学报（英文版）；2009;38。https://doi.org/10.1016/j.ejbt.2025.06.004。

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引用次数: 0

Chufeng Yisun Decoction treats dry eye syndrome by inhibiting the PI3K/Akt pathway 出风益孙汤通过抑制PI3K/Akt通路治疗干眼症

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-08-13 DOI: 10.1016/j.ejbt.2025.05.006

Yue Du , Xue Jiang , Yanyan Zhang , Quanyong Yi

Background

Dry eye disease seriously affects people’s work and life. Chufeng Yisun Decoction is a traditional Chinese medicine decoction used in treating dry eye disease. This study aims to explore the core active ingredients, targets, and mechanisms of CFYSD in dry eye disease, providing new insights for the dry eye disease treatment.

Results

A total of 196 target genes were screened from Chufeng Yisun Decoction, and 170 genes were related to dry eye disease. Gene Ontology and KEGG enrichment analyses showed that Chufeng Yisun Decoction influenced dry eye disease through “Lipid and atherosclerosis”, “Fluid shear stress and atherosclerosis”, and “PI3K-Akt”. The core targets of Chufeng Yisun Decoction in treating dry eye disease were Akt1 and IL-1β. The core active ingredients were kaempferol, wogonin, and quercetin. Molecular docking results showed that the binding energies of kaempferol and Akt1, wogonin and Akt1, quercetin and Akt1, and quercetin and IL-1β were −6.1, −6.1, −6.1, and −7.9 kcal/mol, respectively. Chufeng Yisun Decoction significantly alleviated cell damage and reduced PI3K/Akt pathway-related protein expression. PI3K activation partially reversed the therapeutic effect of Chufeng Yisun Decoction on dry eye disease.

Conclusions

Chufeng Yisun Decoction treats dry eye disease by inactivating the PI3K/Akt pathway through multi-ingredients and multi-targets.

How to cite: Du Y, Jiang X, Zhang Y, et al. Chufeng Yisun Decoction treat dry eye syndrome by inhibiting the PI3K/Akt pathway. Electron J Biotechnol 2025;78. https://doi.org/10.1016/j.ejbt.2025.05.006.

干眼病严重影响人们的工作和生活。出风益孙汤是一种治疗干眼症的中药汤剂。本研究旨在探讨CFYSD在干眼病中的核心活性成分、靶点及作用机制，为干眼病的治疗提供新的见解。结果从出风益孙汤中共筛选出196个靶基因，其中干眼症相关基因170个。基因本体和KEGG富集分析显示，初风益孙汤通过“脂质与动脉粥样硬化”、“流体剪应力与动脉粥样硬化”、“PI3K-Akt”影响干眼症。出风益孙汤治疗干眼症的核心靶点是Akt1和IL-1β。主要活性成分为山奈酚、枸杞素和槲皮素。分子对接结果表明，山奈酚与Akt1、枸杞素与Akt1、槲皮素与Akt1、槲皮素与IL-1β的结合能分别为−6.1、−6.1、−6.1和−7.9 kcal/mol。出风益孙汤明显减轻细胞损伤，降低PI3K/Akt通路相关蛋白表达。PI3K的激活部分逆转了出风益孙汤对干眼症的治疗作用。结论舒风益孙汤通过多成分、多靶点灭活PI3K/Akt通路治疗干眼症。引用方式：杜勇，姜旭，张勇，等。出风益孙汤通过抑制PI3K/Akt通路治疗干眼症。中国生物医学工程学报（英文版）；2009;38。https://doi.org/10.1016/j.ejbt.2025.05.006。

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引用次数: 0

METTL16 promotes osteosarcoma progression by inducing m6A methylation of the UBE3A and Notch signaling pathway METTL16通过诱导UBE3A和Notch信号通路的m6A甲基化来促进骨肉瘤的进展

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-10-09 DOI: 10.1016/j.ejbt.2025.07.006

Yanlin Tan , Jun Gao

Background

N6-methyladenosine (m⁶A) methylation plays a key role in osteosarcoma (OS) progression. This study aimed to elucidate the function and mechanism of methyltransferase 16 (METTL16), an m⁶A methyltransferase, in OS progression.

Results

Bioinformatics analysis with quantitative reverse-transcription polymerase chain reaction (qRT-PCR) revealed high METTL16 expression in OS. After performing cell functional experiments, METTL16 silencing was shown to decrease the proliferation, migration, and invasion of OS cells. Using qRT-PCR, methylated RNA immunoprecipitation quantitative polymerase chain reaction (MeRIP-qPCR), Western blotting, luciferase, RNA-binding protein immunoprecipitation (RIP), and RNA stability assays, METTL16 induced the m⁶A methylation of ubiquitin protein ligase E3A (UBE3A) to promote UBE3A expression and mRNA stability in OS cells in a fragile X messenger ribonucleoprotein 1 (FMR1)-dependent manner. Moreover, in vitro and in vivo results showed that UBE3A activated the Notch signaling pathway, thereby promoting OS cell malignancy. METTL16 knockdown partly reversed the oncogenic role of UBE3A in OS cells.

Conclusions

METTL16 acts as a tumor promotor in OS progression by modulating UBE3A expression via m⁶A methylation to activate the Notch signaling pathway. The findings highlight the therapeutic potential of disrupting the METTL16–UBE3A–Notch pathway axis in OS.

How to cite: Tan Y, Gao J. METTL16 promotes osteosarcoma progression by inducing m6A methylation of the UBE3A and Notch signaling pathway. Electron J Biotechnol 2025;78. https://doi.org/10.1016/j.ejbt.2025.07.006.

n6 -甲基腺苷（m6A）甲基化在骨肉瘤（OS）的进展中起关键作用。本研究旨在阐明m6A甲基转移酶甲基转移酶16 （methyltransferase 16, METTL16）在OS进展中的作用和机制。结果定量逆转录聚合酶链反应（qRT-PCR）生物信息学分析显示METTL16在OS中高表达。细胞功能实验表明，METTL16沉默可降低OS细胞的增殖、迁移和侵袭。通过qRT-PCR、甲基化RNA免疫沉淀定量聚合酶链反应（MeRIP-qPCR）、Western blotting、荧光素酶、RNA结合蛋白免疫沉淀（RIP）和RNA稳定性分析，METTL16诱导泛素蛋白连接酶E3A （UBE3A）的m6A甲基化，以脆性X信使核糖核蛋白1 （FMR1）依赖的方式促进UBE3A在OS细胞中的表达和mRNA稳定性。此外，体外和体内实验结果表明，UBE3A激活Notch信号通路，从而促进OS细胞恶性。METTL16敲低部分逆转了UBE3A在OS细胞中的致癌作用。结论smettl16通过m6A甲基化调节UBE3A表达激活Notch信号通路，在OS进展中发挥肿瘤启动子作用。这些发现强调了破坏METTL16-UBE3A-Notch通路轴在OS中的治疗潜力。METTL16通过诱导UBE3A和Notch信号通路的m6A甲基化促进骨肉瘤的进展。中国生物医学工程学报（英文版）；2009;38。https://doi.org/10.1016/j.ejbt.2025.07.006。

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引用次数: 0

Kaixin San in treating vascular dementia via regulating the Bcl-2/Beclin-1/LC3A/B signaling pathway via animal experiments and network pharmacology analysis 动物实验及网络药理学分析表明开心散通过调节Bcl-2/Beclin-1/LC3A/B信号通路治疗血管性痴呆

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-10-15 DOI: 10.1016/j.ejbt.2025.08.001

Meng-qi Li , Yu-cheng Lu , Yan-chun Li , Yu-fu Zeng , Ying-ying Cao , Ling Zhang , Ben Chen , Ling Chen , Wei-an Qiu , Zi-heng Huang

Background

Kaixin San (KXS), a traditional Chinese herbal formula, is used to treat vascular dementia (VaD), but its active ingredients and mechanisms remain unclear. This study combined animal experiments with network pharmacology to explore how KXS modulates the Bcl-2/Beclin-1/LC3A/B pathway in VaD treatment.

Results

LC‒MS/MS identified 164 active ingredients in the KXS ethanol extract. In 2-vessel occlusion model rats, KXS significantly improved learning and memory (p < 0.05 or p < 0.01) and reduced hippocampal CA1 neuronal damage. Western blotting showed KXS upregulated Bcl-2/Bcl-XL and downregulated Beclin-1, LC3A/B, and Bax (p < 0.05). Immunofluorescence confirmed increased Bcl-2 and decreased Beclin-1 expression. Network pharmacology predicted 73 targets and 174 pathways, with TNF, AKT1, IL-1β, PTGS2, and ESR1 as key targets. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis linked these targets to cancer, atherosclerosis, and AGE-RAGE signaling pathways.

Conclusions

KXS alleviates VaD by modulating autophagy and apoptosis via Bcl-2/Beclin-1/LC3A/B, with multi-target, multi-pathway effects. These findings support further investigation of KXS as a potential therapy for VaD.

How to cite: Li M, Lu Y, Li Y, et al. Kaixin San in treating vascular dementia via regulating the Bcl-2/Beclin-1/LC3A/B signaling pathway via animal experiments and network pharmacology analysis. Electron J Biotechnol 2025;78. https://doi.org/10.1016/j.ejbt.2025.08.001.

开心散（KXS）是一种治疗血管性痴呆（VaD）的传统中药配方，但其有效成分和作用机制尚不清楚。本研究将动物实验与网络药理学相结合，探讨KXS如何调节Bcl-2/Beclin-1/LC3A/B通路在VaD治疗中的作用。结果薄层色谱-质谱联用技术鉴定出了164种有效成分。两支血管闭塞模型大鼠，KXS显著改善学习记忆（p <； 0.05或p <； 0.01），减轻海马CA1神经元损伤。Western blotting显示KXS上调Bcl-2/Bcl-XL，下调Beclin-1、LC3A/B和Bax （p < 0.05）。免疫荧光证实Bcl-2表达升高，Beclin-1表达降低。网络药理学预测73个靶点和174条通路，其中TNF、AKT1、IL-1β、PTGS2和ESR1是关键靶点。京都基因和基因组百科全书（KEGG）分析将这些靶点与癌症、动脉粥样硬化和AGE-RAGE信号通路联系起来。结论skxs通过Bcl-2/Beclin-1/LC3A/B调控VaD的自噬和凋亡，具有多靶点、多通路的作用。这些发现支持进一步研究KXS作为VaD的潜在治疗方法。引用方式：李敏，陆毅，李毅，等。动物实验及网络药理学分析表明开心散通过调节Bcl-2/Beclin-1/LC3A/B信号通路治疗血管性痴呆。中国生物医学工程学报（英文版）；2009;38。https://doi.org/10.1016/j.ejbt.2025.08.001。

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引用次数: 0

Circulating exosomal miRNA signatures as potential biomarkers and therapeutic targets in biliary colic 循环外泌体miRNA特征作为胆绞痛的潜在生物标志物和治疗靶点

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology

Pub Date : 2025-11-01 Epub Date: 2025-08-13 DOI: 10.1016/j.ejbt.2025.05.007

Xiangjie Han, Anshi Wu, Mengmeng Bao

Background

Biliary colic (BC), characterized by intermittent pain due to gallstone-related bile duct obstruction, remains poorly understood at the molecular level. Circulating exosomal microRNAs (miRNAs) have emerged as potential biomarkers for various diseases. This study aimed to identify exosomal miRNA profiles in BC patients and explore their therapeutic implications.

Results

Analysis of plasma exosomal miRNAs from 10 BC patients during acute attacks and 10 healthy controls (HCs) revealed distinct expression patterns separating BC from HC groups. Integration of differential expression analysis, WGCNA, and LASSO regression identified 7 key miRNAs (hsa-miR-142-3p, hsa-miR-32-5p, hsa-miR-374a-3p, hsa-miR-155-5p, hsa-miR-425-3p, hsa-miR-584-5p, hsa-miR-185-5p) strongly associated with BC. Support vector machine models using these miRNAs achieved excellent diagnostic performance (AUC = 1.0, where AUC represents Area Under the Curve). miRNA-targeting drugs including Remlarsen and Cobomarsen showed potential for therapeutic intervention.

Conclusions

This study identified specific exosomal miRNA signatures that distinguish BC patients from HC and revealed potential miRNA-targeting therapeutics. These findings advance our understanding of BC pathophysiology and provide direction for developing novel diagnostics and treatments.

How to cite: Han X, Wu A, Bao M. Circulating exosomal miRNA signatures as potential biomarkers and therapeutic targets in biliary colic. Electron J Biotechnol 2025;77. https://doi.org/10.1016/j.ejbt.2025.05.007.

胆道绞痛（BC）以胆结石相关胆管阻塞引起的间歇性疼痛为特征，在分子水平上仍然知之甚少。循环外泌体microRNAs （miRNAs）已成为多种疾病的潜在生物标志物。本研究旨在鉴定BC患者的外泌体miRNA谱并探讨其治疗意义。结果对10例急性发作的BC患者和10例健康对照（HC）的血浆外泌体mirna进行分析，发现BC组和HC组的表达模式不同。结合差异表达分析、WGCNA和LASSO回归，确定了7个与BC密切相关的关键mirna （hsa-miR-142-3p、hsa-miR-32-5p、hsa-miR-374a-3p、hsa-miR-155-5p、hsa-miR-425-3p、hsa-miR-584-5p、hsa-miR-185-5p）。使用这些mirna的支持向量机模型获得了出色的诊断性能（AUC = 1.0，其中AUC表示曲线下面积）。mirna靶向药物包括Remlarsen和Cobomarsen显示出治疗干预的潜力。本研究确定了区分BC患者和HC患者的特异性外泌体miRNA特征，并揭示了潜在的miRNA靶向治疗方法。这些发现促进了我们对BC病理生理学的理解，并为开发新的诊断和治疗方法提供了方向。韩霞，吴安，鲍敏。循环外泌体miRNA标记作为胆道绞痛的潜在生物标志物和治疗靶点。中国生物医学工程学报（英文版）；2009;77。https://doi.org/10.1016/j.ejbt.2025.05.007。

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引用次数: 0