Electronic Journal of Biotechnology最新文献

{"title":"Application of mitochondrial genomes to species identification and evolution","authors":"Bingpeng Xing ,&nbsp;Liangyu Lin ,&nbsp;Qiong Wu","doi":"10.1016/j.ejbt.2025.04.001","DOIUrl":"10.1016/j.ejbt.2025.04.001","url":null,"abstract":"<div><div>Mitochondrial genomes (mtDNA) have become invaluable in species classification and evolutionary studies due to their unique characteristics, including maternal inheritance, and high mutation rates. This review examines the application of mtDNA in tracing evolutionary history, elucidating phylogenetic relationships, and understanding mechanisms of species divergence. The evolution of mitochondrial DNA research from its initial focus on energy metabolism to its current role in biodiversity assessments highlights its significance in modern biology. Mitochondrial DNA barcoding, particularly utilizing the cytochrome <em>c</em> oxidase I (COI) gene, has revolutionized species identification, enabling rapid and accurate classification across diverse taxa. The article further explores the implications of mtDNA in understanding adaptive evolution, as genetic variations within mitochondrial genomes can reveal insights into how species respond to environmental pressures. However, challenges such as gene mixing, hybridization, and incomplete lineage sorting can complicate interpretations of mtDNA data. Thus, integrating mitochondrial with nuclear genome data is advocated to provide a comprehensive view of species relationships and evolutionary patterns. Future research directions emphasize the need for multi-genome studies, investigations into ecological adaptations, and exploration of understudied taxa and ecosystems, which are crucial for enhancing our understanding of biodiversity and informing conservation strategies.</div><div><strong>How to cite:</strong> Xing B, Lin L, Wu Q. Application of mitochondrial genomes to species identification and evolution. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.04.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 39-48"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of isothermal amplification technology: A bibliometric study (2013–2022)","authors":"Hanting Zhu ,&nbsp;Zhitong Miao ,&nbsp;Junfei Yuan ,&nbsp;Wanying Xie ,&nbsp;Qiaoqiao Zhang ,&nbsp;Kun Yang","doi":"10.1016/j.ejbt.2025.03.003","DOIUrl":"10.1016/j.ejbt.2025.03.003","url":null,"abstract":"<div><h3>Background</h3><div>Isothermal amplification is a nucleic acid amplification technology (NAAT) that has contributed significantly to molecular diagnostics. The combination of NAAT with a suitable detection platform has improved sensitivity and specificity and enabled rapid disease diagnosis. A total of 5388 articles relating to isothermal amplification technology published from 2013 to 2022 were identified in the Web of Science Core Collection (WoSCC) database and subsequently analyzed with CiteSpace 5.7.R1 software.</div></div><div><h3>Results</h3><div>The number of published articles on isothermal amplification technology steadily increased between 2013 and 2022. The disciplines included Chemistry, Science Technology Other Topics, Biotechnology Applied Microbiology, Microbiology, and Biochemistry Molecular Biology. The countries with the highest number of published articles were China, the United States, and Japan while the institutions with the highest number of published articles were the Chinese Academy of Sciences, the Chinese Academy of Agricultural Sciences, and Mahidol University. The high-frequency keywords included rapid detection, sensitivity, pathogen, <em>Escherichia coli</em>, probe, primer, and expression. Over recent years, most research studies concerning isothermal amplification focused on its advantages, improvements, and applications. RCA, LAMP, RPA, and Cas technologies appeared sequentially from 2013 onward. The keyword “LAMP” exhibited the highest frequency (1222 times).</div></div><div><h3>Conclusions</h3><div>Our study described the research trends and the status of isothermal amplification technology over the past decade, providing guidance for future research in this field.</div><div><strong>How to cite:</strong> Zhu H, Miao Z, Yuan J, et al. The evolution of isothermal amplification technology: A bibliometric study (2013–2022). Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.003</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 12-21"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the process conditions for the biosorption of chromium (VI) by Bacillus subtilis in artificial wastewater","authors":"Khawla E. Alsamhary","doi":"10.1016/j.ejbt.2025.03.005","DOIUrl":"10.1016/j.ejbt.2025.03.005","url":null,"abstract":"<div><h3>Background</h3><div>The contamination by heavy metals, particularly hexavalent chromium Cr (VI), is a pressing environmental concern. Cr(VI) is highly toxic, non-biodegradable and carcinogenic. Traditional remediation methods are often costly, energy-intensive, or generate secondary waste. This study explores the optimal conditions for the bacterium <em>Bacillus subtilis</em> in reducing Cr(VI) from synthetic wastewater.</div></div><div><h3>Results</h3><div>The research employed batch experiments to simulate wastewater treatment. The removal of Cr(VI) was measured spectrophotometrically. The active functional groups were studied using Fourier Transform Infrared Spectroscopy that showed an incremental shift for alkyl halides (500.75 cm⁻¹) and OH-groups (3347 cm⁻¹) were observed. Scanning Electron Microscopy images demonstrated that the surface morphology of the biosorbent was more homogenous before than after adsorption. The biosorbent’s structure was confirmed by a prominent peak in X-ray Diffraction at 290.04°. The highest adsorption was observed at the adsorbent dose of 0.5 g/L, the contact time 60 min, pH 6 and temperature of 40°C. The thermodynamic parameters validated the process’s feasibility and spontaneity. Several models for biosorption kinetics and isotherm were tested. The pseudo-second-order was more suitable than the pseudo-first-order model. Langmuir isotherm model had the best fit compared to Freundlich, Dubinin–Radushkevich, and Temkin models.</div></div><div><h3>Conclusions</h3><div><em>B. subtilis</em> appeared to be resistant to chromium and reduce Cr(VI) efficiently. This study shows the potential of <em>B. subtilis</em> as a viable bioremediation agent for Cr(VI) contamination in wastewater and should be studied further using real wastewater with different pollutants.</div><div><strong>How to cite:</strong> Alsamhary KE. Optimizing the process conditions for the biosorption of chromium (VI) by <em>Bacillus subtilis</em> in artificial wastewater. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 22-38"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of heterotrophic nitrifying/aerobic denitrifying strain Pseudomonas stutzeri YXH-102 to hypersaline stress","authors":"Youming Wang ,&nbsp;Zhichun Wang ,&nbsp;Yangfan Hu ,&nbsp;Dexun Zou ,&nbsp;Korakot Sombatmankhong ,&nbsp;Wen Wang ,&nbsp;Bo Yu","doi":"10.1016/j.ejbt.2025.05.001","DOIUrl":"10.1016/j.ejbt.2025.05.001","url":null,"abstract":"<div><h3>Background</h3><div>Hypersaline wastewater poses significant environmental challenges, necessitating efficient bioremediation strategies. This study investigates the hypersaline tolerance mechanisms of <em>Pseudomonas stutzeri</em> YXH-102, a heterotrophic nitrifying/aerobic denitrifying bacterium isolated from Yuncheng Salt Lake sediments, under high-salinity stress.</div></div><div><h3>Results</h3><div>Comparative transcriptomic analysis revealed 268 differentially expressed genes (DEGs) in response to a 10 % NaCl shock, with 86 upregulated and 182 downregulated. Key findings highlight the critical roles of oxidative stress mitigation, energy metabolism adaptation, and ion homeostasis. Salt stress triggered reactive oxygen species (ROS) accumulation, countered by upregulated cytochrome <em>c</em> oxidase (reducing ROS generation) and glutathione S-transferase (enhancing ROS scavenging). Concurrently, energy metabolism pathways, including fatty acid β-oxidation and acetyl-CoA production, were activated to sustain cellular energy demand. Notably, the electron transport chain (ETC) generated a robust proton motive force (PMF), which directly fueled potassium uptake via H⁺/K⁺ symporters to counteract osmotic imbalance. TonB-dependent transporters for nutrient uptake were also significantly upregulated, suggesting enhanced nutrient acquisition under salinity.</div></div><div><h3>Conclusions</h3><div>These findings elucidate how <em>P. stutzeri</em> YXH-102 combats salt stress through integrated ROS detoxification, energy optimization, and PMF-driven ion transport, providing molecular insights for its application in hypersaline wastewater bioremediation.</div><div><strong>How to cite:</strong> Wang Y, Wang Z, Hu Y, et al. Response of heterotrophic nitrifying/aerobic denitrifying strain <em>Pseudomonas stutzeri</em> YXH-102 to hypersaline stress. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.05.001</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 49-56"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin mitigates potassium bromate-induced liver grievance in rat through attenuating NF-kB and PI3K/Akt pathway","authors":"Bo Ma ,&nbsp;Sheng Zheng ,&nbsp;Ning Xie ,&nbsp;Juan Yang ,&nbsp;Xueli Zeng ,&nbsp;Pei Liu ,&nbsp;Shunling Zhang ,&nbsp;Ji Li","doi":"10.1016/j.ejbt.2025.03.002","DOIUrl":"10.1016/j.ejbt.2025.03.002","url":null,"abstract":"<div><h3>Background</h3><div>Metformin (MET) is a dietary polyphenolic compound that exhibits anti-inflammatory and antioxidant properties. This study evaluated the protective effects of MET in both <em>in vitro</em> and <em>in vivo</em> models against potassium bromate (KBrO₃)-induced hepatotoxicity. Hepatic cells were exposed to KBrO₃ with or without metformin (20, 40, and 60 µM), and cell viability and Reactive Oxygen Species levels were assessed. <em>In vivo,</em> rats were divided into five groups: control, KBrO₃, and KBrO₃ with metformin (25, 50, and 100 mg/kg). Liver and blood samples were analyzed for histological changes, oxidative stress markers, lipid peroxidation, liver enzymes, and PI3K/Akt signaling.</div></div><div><h3>Results</h3><div>KBrO₃ exposure significantly decreased cell viability and increased ROS levels. Co-treatment with MET dose-dependently restored cell viability, with 60 µM MET achieving approximately 80% viability. Metformin also reduced ROS levels, with mean fluorescence intensity approaching control values at higher concentrations. In the <em>in vivo</em> study, KBrO₃ exposure elevated lipid peroxidation markers, depleted antioxidant enzyme activities, and triggered oxidative stress and inflammation. Metformin significantly alleviated histological liver damage, suppressed proinflammatory cytokines, enhanced antioxidant enzyme activities, and modulated the PI3K/Akt signaling pathway to promote cell survival and reduce oxidative injury.</div></div><div><h3>Conclusions</h3><div>Metformin effectively protects hepatic cells against KBrO₃-induced cytotoxicity by improving cell viability and reducing Reactive Oxygen Species levels. Metformin successfully mitigates KBrO₃-induced hepatic injury by reducing oxidative stress, modulating inflammatory pathways (NF-kB), and regulating the PI3K/Akt signaling cascade, offering molecular evidence of its hepatoprotective effects.</div><div><strong>How to cite:</strong> Ma B, Zheng S, Xie N, et al. Metformin mitigates potassium bromate induced liver grievance in rat through attenuating NF-kB and PI3K/Akt pathway. Electron J Biotechnol 2025;76. <span><span>https://doi.org/10.1016/j.ejbt.2025.03.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"76 ","pages":"Pages 1-11"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Octoploid blueberry development for drought tolerance: A combined approach of in vitro polyploidization and somatic organogenesis","authors":"Alejandra Araujo Heraldez ,&nbsp;Susana Valdez Peñuelas ,&nbsp;Gabriela Jarpa-Tauler ,&nbsp;Aparna Banerjee ,&nbsp;Kattia Núñez-Montero ,&nbsp;Patricio Arce-Johnson ,&nbsp;Jesús L. Romero-Romero","doi":"10.1016/j.ejbt.2025.01.004","DOIUrl":"10.1016/j.ejbt.2025.01.004","url":null,"abstract":"<div><h3>Background</h3><div>The blueberry (<em>Vaccinium</em> spp.) is a fruit commercially known for its high quality and health benefits, particularly for its bioactive antioxidant compounds, which are important in the medical field. However, factors such as genotype, stage of fruit ripening and environmental conditions impact the biosynthesis of bioactive compounds in the berry, as well as their yield and cultivation costs. In Mexico, particularly in the state of Sinaloa, extreme climatic conditions limit the cultivation of blueberry and highlight the need for the development of new varieties with low chilling requirements and tolerance to drought conditions.</div></div><div><h3>Results</h3><div>Through the combined use of somatic organogenesis and <em>in vitro</em> polyploidization, genetic variability was promoted in the commercial blueberry plant variety “Biloxi”. To achieve this purpose, blueberry microcuttings were treated with colchicine (0.02%) for six hours for 2, 4, 6 and 8 consecutive days and induced to form shoots <em>in vitro</em> with Zeatin (1 mg·L⁻¹). Out of 304 generated plants, 36 showed lower stomatal density and 9 lines showed higher stomatal density. Likewise, 5 and 49 lines presented lower and larger stomatal sizes, respectively. In 9 lines, a higher chlorophyll content was found (10% to 200%) compared to the control treatment. Ploidy analysis using flow cytometry showed the successful generation of four octoploid blueberry plants.</div></div><div><h3>Conclusions</h3><div>This work successfully generated new octoploid blueberry plants. Currently, all the lines that presented histological, biochemical and/or genetic modifications are being evaluated under greenhouse conditions for fruit quality and drought tolerance.</div><div><strong>How to cite:</strong> Heraldez AA, Peñuelas SV, Jarpa-Tauler G, et al. Octoploid blueberry development for drought tolerance: A combined approach of <em>in vitro</em> polyploidization and somatic organogenesis. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.004</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 20-27"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Erodium glaucophyllum (L.) Aiton growing in arid environment: Phytochemical characterization, antimicrobial, antioxidant, and anticancer potential","authors":"Amr H. Hashem ,&nbsp;Bahaa M. Badr ,&nbsp;Fathy M. Elkady ,&nbsp;Mostafa A. Abdel-Maksoud ,&nbsp;Abdulaziz Alamri ,&nbsp;Mohamed A. El-Tayeb ,&nbsp;Bushra H. Kiani ,&nbsp;Amer M. Abdelaziz","doi":"10.1016/j.ejbt.2025.01.005","DOIUrl":"10.1016/j.ejbt.2025.01.005","url":null,"abstract":"<div><h3>Background</h3><div>The misuse of antimicrobial agents resulted in a global serious health concern, namely antimicrobial resistance. Also, traditional bioactive compounds are associated with undesirable adverse effects. This study aimed to first assess the phytochemical analyses and biological activities of <em>Erodium glaucophyllum</em> (L.) Aiton from the arid region.</div></div><div><h3>Results</h3><div>The gas chromatography-mass spectroscopy analysis of <em>Erodium glaucophyllum</em> leaves crude extract revealed the presence of 36 biologically vital compounds, with 8 main compounds being identified. Docosenamide, hexadecanoic acid, thiocarbamic acid, hentriacontane, β-sitosterol, quinoline, and oleic acid were among the most significant compounds. Docosenamide was the most prevalent compound, comprising 45.3%. The phytochemical analysis revealed the presence of a diverse array of chemical compounds, such as carbohydrates, polyphenols, flavonoids, and tannins. In moderate concentrations, saponins, glycosides, quinones, proteins, and amino acids were present. Additionally, alkaloids, steroids, diterpenes, and cardiac glycosides were identified in trace amounts. Also, chlorogenic acid was the dominant with 69.14% among other phenolic compounds. The antimicrobial results of the tested extract showed promising activity against <em>Bacillus subtilis, Staphylococcus aureus, Salmonella typhimurium,</em> and <em>Pseudomonas aeruginosa</em>, with minimum inhibitory concentration of 31.25, 15.62, 15.62, and 62.5 µg/ml, respectively. Furthermore, the extract demonstrated potent antioxidant activity, with an EC50 of 51.7 µg/ml, and anticancer activity against MCF-7 malignant cell line, with an IC50 of 58.4 µg/ml.</div></div><div><h3>Conclusions</h3><div>The tested crude extract of <em>Erodium glaucophyllum</em> leaves represents a potential source of bioactive compounds that possess antimicrobial, antioxidant, and anticancer properties.</div><div><strong>How to cite:</strong> Hashem AH, Badr BM, Elkady FM, et al. Novel <em>Erodium glaucophyllum</em> (L.) Aiton growing in arid environment: Phytochemical characterization, antimicrobial, antioxidant, and anticancer potential. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.005</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 57-68"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient production of 6-hydroxynicotinic acid by newly isolated Pseudomonas poae","authors":"Yi Li ,&nbsp;Jiacheng Tang ,&nbsp;Kaixiang Xin ,&nbsp;Zongda Chen ,&nbsp;Lele Zhao ,&nbsp;Yifan Zhao ,&nbsp;Yinbiao Xu ,&nbsp;Pei Zhou ,&nbsp;Yang Sun ,&nbsp;Yupeng Liu ,&nbsp;Hua Li","doi":"10.1016/j.ejbt.2025.01.002","DOIUrl":"10.1016/j.ejbt.2025.01.002","url":null,"abstract":"<div><h3>Background</h3><div>Nicotinic acid dehydrogenase possesses the capability to convert nicotinic acid into 6-hydroxynicotinic acid, a compound of significant research value as a pharmaceutical intermediate. The extraction of nicotinic acid dehydrogenase is primarily performed by strains. However, the enzyme activity of the strains reported currently is relatively low, and their potential to catalyze the production of 6-hydroxynicotinic acid is insufficient to meet industrial requirements.</div></div><div><h3>Results</h3><div>Due to the revealing properties of 6-hydroxynicotinic acid, this study proposes a technique for calculating the luminescence intensity of colonies, which is based on a fluorescence spectrometer. The developed method establishes a reliable linear relationship (88.2%) between the luminescence intensity and enzyme activity. Consequently, it has been employed to screen strains that produce nicotinate dehydrogenase. This screening approach allows for the evaluation of about 500 enzyme-producing strains daily, presenting an efficient strategy for screening.</div></div><div><h3>Conclusions</h3><div>Through this approach, a novel high enzyme activity strain producing nicotinic acid dehydrogenase, <em>Pseudomonas poae</em> have been obtained, which is designated as HD530. After process optimization, it was utilized to produce 6-hydroxynicotinic acid, achieving a high yield of 155.45 g/L within 72 h, meeting the requirements for industrial production. The effectiveness and potential of this technique lie in its application for strain screening and improvement.</div><div><strong>How to cite:</strong> Li Y, Tang J, Xin K, et al. Efficient production of 6-hydroxynicotinic acid by newly isolated <em>Pseudomonas poae</em>. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 1-10"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing conditions for augmented production of amylase by Talaromyces islandicus AUMC 11391","authors":"Osama A.M. Al-Bedak ,&nbsp;Ahmed M. Moharram ,&nbsp;Fuad Ameen ,&nbsp;Steven L. Stephenson ,&nbsp;Marwa M.M. Idres ,&nbsp;Manal M. Yasser","doi":"10.1016/j.ejbt.2025.01.008","DOIUrl":"10.1016/j.ejbt.2025.01.008","url":null,"abstract":"<div><h3>Background</h3><div>To satisfy rising biotechnological needs, climate change, and depleting water supplies, amylases that can survive high temperatures, and salt concentrations must be developed. Amylases are the most important enzymes that comprise approximately 25–33% of the international enzyme market and have a great role in many industries.</div></div><div><h3>Results</h3><div>In this investigation, <em>Talaromyces islandicus</em> AUMC 11391 was used to produce amylase in submerged fermentation (SmF) in an augmented concentration of 232 ± 36 U/mL after 8 d of incubation at pH 6.0 and 30°C using sodium nitrate as a nitrogen supply. The obtained enzyme was partly purified employing 70% ammonium sulfate and then dialysis. The activity of the produced amylase exhibited reached the peak (992.14 ± 80 U/mg protein) at pH 5.0 and 55°C. Cu, Co, Fe, Ni, Ca, and Zn had an activating effect on the activity of the amylase enzyme at pH 5.0 and 55°C by 134.57, 123.1, 115.4, 109.76, 105.43, and 103.2%, respectively. The <em>K</em>_m and <em>V</em>_max were recorded as 132.1 mM and 60.6 µmol/min, respectively. <em>T. islandicus</em> AUMC 11391′s-amylase hydrolyzed the raw starch of maize, sorghum, wheat, rice, and oat at rates of 12.3, 113.7, 32.25, 34.67, and 73.6% in contrast to the soluble starch.</div></div><div><h3>Conclusions</h3><div>This enhanced α-amylase from <em>T. islandicus</em> AUMC 11391 looks to be a potential option to meet the present amylase requirements of a variety of industrial processes because of its improved production and beneficial properties.</div><div><strong>How to cite:</strong> Al - Bedak O.A.M., Moharram AM., Ameen F, et al. Optimizing conditions for augmented production of amylase by <em>Talaromyces islandicus</em> AUMC 11391. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.008</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 39-48"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection and validation of stable reference genes in guava (Psidium guajava L.) for reliable and consistent gene expression analysis","authors":"Sandeep Kumar ,&nbsp;Manoharan Muthukumar ,&nbsp;Anju Bajpai ,&nbsp;Amar Kant Kushwaha ,&nbsp;Israr Ahmad ,&nbsp;Yashi Bajpai ,&nbsp;Anshuman Singh ,&nbsp;Thukkaram Damodaran ,&nbsp;Mala Trivedi","doi":"10.1016/j.ejbt.2025.01.006","DOIUrl":"10.1016/j.ejbt.2025.01.006","url":null,"abstract":"<div><h3>Background</h3><div>Ideal stably expressing housekeeping gene to be used as internal control needs to be optimized for efficient gene expression analysis for accurate mRNA quantitation. In pursuit of identifying suitable reference genes for gene expression analysis in guava, a systematic study was conducted using different tissues of guava cv. Allahabad Safeda examining 10 housekeeping genes such as Actin (<em>PgACT</em>), Elongation factor 1G (<em>PgEF1G</em>), Elongation factor 2 (<em>PgEF2</em>), Tubulin (<em>PgTUB1</em>), Elongation factor 1 α (<em>PgEF1a</em>), Monensin sensitivity1 (<em>PgMON1</em>), Histone H3 (<em>PgH3</em>), RNA-binding protein 47 (<em>PgRBP47</em>), Polyubiquitin_X1 (<em>PgPOLX1</em>), and Polyubiquitin_X2 (<em>PgPOLX2</em>).</div></div><div><h3>Results</h3><div>qRT-PCR analysis showed amplification efficiencies ranging from 74.4% to 124.9%, with correlation coefficients exceeding 0.98. The stability of these genes’ expression was evaluated using six methods: GeNorm, NormFinder, BestKeeper, RefFinder, comparative delta-Ct, and Stability index in which different methods identified <em>PgRBP47</em> as least stable and indicated its unsuitability as a reference gene but showed variations in the ranking of the genes for gene stability.</div></div><div><h3>Conclusions</h3><div>Comparison of all the methods and accounting for the top 3 ranks of gene stability, three genes i.e., <em>PgTUB1</em>, <em>PgEF1a</em>, and <em>PgEF2</em> were identified as more stable housekeeping genes across different tissues of guava and could be considered as ideal reference genes for normalization in gene expression studies of guava.</div><div><strong>How to cite:</strong> Kumar S, Muthukumar M, Bajpai A, et al. Selection and validation of stable reference genes in guava (<em>Psidium guajava</em> L.) for reliable and consistent gene expression analysis. Electron J Biotechnol 2025;75. <span><span>https://doi.org/10.1016/j.ejbt.2025.01.006</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"75 ","pages":"Pages 49-56"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}