首页 > 最新文献

Egyptian Journal of Zoology最新文献

英文 中文
SUSCEPTIBILITY OF THE DIFFERENT STAGES OF THE MEDFLY CERATITIS CAPITATA WIEDEMANN (DIPTERA: TEPHRITIDAE) TO THE EXTRACTS OF VIOLA ODORATA AND EUCALYPTUS CAMALDEULENSIS 不同阶段头角蝇(双翅目:绢蝇科)对堇菜和桉叶提取物的敏感性
Pub Date : 2023-01-20 DOI: 10.21608/ejz.2022.169125.1089
A. Ibrahim, N. Soliman, Sherihan Alamin, A. Mesbah, Ali M. A. Mahmoud
ABSTRACT
摘要
{"title":"SUSCEPTIBILITY OF THE DIFFERENT STAGES OF THE MEDFLY CERATITIS CAPITATA WIEDEMANN (DIPTERA: TEPHRITIDAE) TO THE EXTRACTS OF VIOLA ODORATA AND EUCALYPTUS CAMALDEULENSIS","authors":"A. Ibrahim, N. Soliman, Sherihan Alamin, A. Mesbah, Ali M. A. Mahmoud","doi":"10.21608/ejz.2022.169125.1089","DOIUrl":"https://doi.org/10.21608/ejz.2022.169125.1089","url":null,"abstract":"ABSTRACT","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89629314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE PROFICIENCY OF SILVER NANOPARTICLES IN CONTROLLING COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.), UNDER THE LABORATORY CONDITIONS 在实验室条件下,银纳米颗粒对棉花叶虫的防治效果
Pub Date : 2023-01-02 DOI: 10.21608/ejz.2022.174453.1090
M. Abdou, Ola H. Zyaan
{"title":"THE PROFICIENCY OF SILVER NANOPARTICLES IN CONTROLLING COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.), UNDER THE LABORATORY CONDITIONS","authors":"M. Abdou, Ola H. Zyaan","doi":"10.21608/ejz.2022.174453.1090","DOIUrl":"https://doi.org/10.21608/ejz.2022.174453.1090","url":null,"abstract":"","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81692438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
HISTOLOGY, HISTOCHEMISTRY, AND ULTRASTRUCTURE OF STEROIDOGENESIS DURING TESTICULAR CYCLE OF MUGIL CEPHALUS IN THE NATURAL HABITAT 自然栖息地头螺睾丸周期中甾体生成的组织学、组织化学和超微结构
Pub Date : 2022-10-13 DOI: 10.21608/ejz.2022.149916.1085
F. El-messady, Noha Khalil, M. Mousa
Recently, an increasing attention has been paid to the productivity and propagation of grey mullet “ Mugil cephalus ”. In view of the fact that the steroidogenic hormones play a key role in fish maturation and production of fertile ova, the present work was planned to determine and investigate the steroidogenic cells in the testes of grey mullet, and thence to determine their levels of activities during its maturing cycle, aiming at making use of the obtained data in fish breeding and culture. Mature males of M. cephalus were obtained from the natural habitat (Al Bardawil Lagoon). Cytochemical and ultrastructural investigations were performed for the steroidogenic cells containing 3β-hydroxysteroid dehydrogenase. Five developmental stages were quite distinguishable in M. cephalus males during the testicular cycle: immature stage, stimulating spermatogenesis, rapid spermatogenesis, mature or ripe, and spent stages. The electron microscopy showed that the interstitial (Leydig), lobular bounding, and Sertoli cells inspected during rapid spermatogenesis displayed prominent lipid droplets and cytoplasmic organoids typical of protein-synthesizing cells (i.e. rough endoplasmic reticulum, abundant ribosomes, and distinct Golgi apparatus). In addition, Sertoli cells, in particular, possessed certain microtubules that are known to serve in transporting metabolites. Furthermore, the testes examined after spawning (spent ones) by electron microscope have designated that Sertoli cells, as well as some Leydig cells, had been changed into phagocytic ones to get ride from the non-ejaculated sperm cells. In conclusion, the present results could be very valuable in the problems concerning fish maturation in captivity.
近年来,人们对“头乌鱼”的产量和繁殖越来越关注。鉴于类固醇激素在鱼类成熟和可育卵子的产生中起着关键作用,本研究拟对灰鲻鱼睾丸中的类固醇细胞进行测定和研究,从而确定其在成熟周期中的活性水平,以期在鱼类育种和养殖中利用所获得的数据。从自然生境(Al Bardawil Lagoon)获得了成熟雄头足鼠。对含有3β-羟基类固醇脱氢酶的细胞进行细胞化学和超微结构研究。雄性头螺在睾丸周期中有5个明显的发育阶段:未成熟期、刺激生精期、快速生精期、成熟或成熟期和花期。电镜显示,在快速精子发生过程中,间质细胞(间质细胞)、小叶结合细胞和支持细胞显示出明显的脂滴和典型的蛋白质合成细胞的细胞质类器官(即粗糙的内质网、丰富的核糖体和明显的高尔基体)。此外,特别是支持细胞,具有某些已知用于运输代谢物的微管。此外,在电子显微镜下对受精后的睾丸(废睾丸)进行了检查,发现支持细胞和一些间质细胞已经变成了吞噬细胞,以取代非射精的精子细胞。综上所述,本研究结果对解决人工养殖鱼类成熟问题具有重要的参考价值。
{"title":"HISTOLOGY, HISTOCHEMISTRY, AND ULTRASTRUCTURE OF STEROIDOGENESIS DURING TESTICULAR CYCLE OF MUGIL CEPHALUS IN THE NATURAL HABITAT","authors":"F. El-messady, Noha Khalil, M. Mousa","doi":"10.21608/ejz.2022.149916.1085","DOIUrl":"https://doi.org/10.21608/ejz.2022.149916.1085","url":null,"abstract":"Recently, an increasing attention has been paid to the productivity and propagation of grey mullet “ Mugil cephalus ”. In view of the fact that the steroidogenic hormones play a key role in fish maturation and production of fertile ova, the present work was planned to determine and investigate the steroidogenic cells in the testes of grey mullet, and thence to determine their levels of activities during its maturing cycle, aiming at making use of the obtained data in fish breeding and culture. Mature males of M. cephalus were obtained from the natural habitat (Al Bardawil Lagoon). Cytochemical and ultrastructural investigations were performed for the steroidogenic cells containing 3β-hydroxysteroid dehydrogenase. Five developmental stages were quite distinguishable in M. cephalus males during the testicular cycle: immature stage, stimulating spermatogenesis, rapid spermatogenesis, mature or ripe, and spent stages. The electron microscopy showed that the interstitial (Leydig), lobular bounding, and Sertoli cells inspected during rapid spermatogenesis displayed prominent lipid droplets and cytoplasmic organoids typical of protein-synthesizing cells (i.e. rough endoplasmic reticulum, abundant ribosomes, and distinct Golgi apparatus). In addition, Sertoli cells, in particular, possessed certain microtubules that are known to serve in transporting metabolites. Furthermore, the testes examined after spawning (spent ones) by electron microscope have designated that Sertoli cells, as well as some Leydig cells, had been changed into phagocytic ones to get ride from the non-ejaculated sperm cells. In conclusion, the present results could be very valuable in the problems concerning fish maturation in captivity.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"63 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78624238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PARASITIC HELMINTHS OF THE FRESHWATER CATFISH (CLARIAS GARIEPINUS) FROM FAYOUM GOVERNORATE, EGYPT 埃及法尤姆省淡水鲶鱼(clarias gariepinus)的寄生蠕虫
Pub Date : 2022-10-13 DOI: 10.21608/ejz.2022.150510.1086
D. Al-Bassel, S. Abo-elhasan, H. Abdel-Halim, Marwa Atwa
The parasitic diseases are one of the potential factors that restrict the development of fish aquaculture because fish parasites not only can cause injuries, but can even lead to death of the fish. The present study aimed to record/update the prevalence and intensity of parasitic helminths in the freshwater catfish " Clarias gariepinus " in Fyoum Governorate, Egypt. A total of 368 Clarias gariepinus samples were collected from Bahr Youssef (line of the River Nile) from Fayoum Governorate during the period from January 2019 to February 2020, and were examined for the presence of helminth parasites. Out of 368 examined fish, 227 (61.7%) were found to be infected with six species of helminthes (three trematodes, two cestodes, and one nematode): Dactylogyrus claridii , Acanthostomum spiniceps , Orientocreadium batrachoides , Polyonchobothrium clarias, Monobothrium sp., and Paracamallanus cythopharynx with infection rates = 13.0%, 2.2%, 5.4%, 9.2%, 13.3%, and 18.5%, respectively. Regarding to the seasonal variations, the obtained results showed that the highest rate of infection was recorded during the spring (76.9%), followed by the summer (70.0%) and the winter (53.5%), while the autumn represented the lowest rate of infection (52.3%). In addition, this study provides additional morphological characters for the parasite Paracamallanus cyathopharynx based on the scanning electron microscopy.
寄生虫病是制约鱼类养殖发展的潜在因素之一,因为鱼类寄生虫不仅会造成伤害,甚至会导致鱼类死亡。本研究旨在记录/更新埃及Fyoum省淡水鲶鱼“Clarias gariepinus”中寄生蠕虫的流行率和强度。2019年1月至2020年2月期间,在法尤姆省巴尔尤塞夫(尼罗河沿岸)共采集了368份加里平克拉丽虫样本,并检查了寄生虫的存在。在368条检测鱼中,227条(61.7%)感染了6种蠕虫(3种吸虫、2种囊虫和1种线虫),分别是:claridii Dactylogyrus、Acanthostomum spiniceps、orienttocreadium batrachoides、Polyonchobothrium clarias、Monobothrium sp.和Paracamallanus cythopharynx,感染率分别为13.0%、2.2%、5.4%、9.2%、13.3%和18.5%。从季节变化来看,春季感染率最高(76.9%),其次是夏季(70.0%)和冬季(53.5%),秋季感染率最低(52.3%)。此外,本研究还提供了基于扫描电镜的cyathopharynx Paracamallanus的附加形态学特征。
{"title":"PARASITIC HELMINTHS OF THE FRESHWATER CATFISH (CLARIAS GARIEPINUS) FROM FAYOUM GOVERNORATE, EGYPT","authors":"D. Al-Bassel, S. Abo-elhasan, H. Abdel-Halim, Marwa Atwa","doi":"10.21608/ejz.2022.150510.1086","DOIUrl":"https://doi.org/10.21608/ejz.2022.150510.1086","url":null,"abstract":"The parasitic diseases are one of the potential factors that restrict the development of fish aquaculture because fish parasites not only can cause injuries, but can even lead to death of the fish. The present study aimed to record/update the prevalence and intensity of parasitic helminths in the freshwater catfish \" Clarias gariepinus \" in Fyoum Governorate, Egypt. A total of 368 Clarias gariepinus samples were collected from Bahr Youssef (line of the River Nile) from Fayoum Governorate during the period from January 2019 to February 2020, and were examined for the presence of helminth parasites. Out of 368 examined fish, 227 (61.7%) were found to be infected with six species of helminthes (three trematodes, two cestodes, and one nematode): Dactylogyrus claridii , Acanthostomum spiniceps , Orientocreadium batrachoides , Polyonchobothrium clarias, Monobothrium sp., and Paracamallanus cythopharynx with infection rates = 13.0%, 2.2%, 5.4%, 9.2%, 13.3%, and 18.5%, respectively. Regarding to the seasonal variations, the obtained results showed that the highest rate of infection was recorded during the spring (76.9%), followed by the summer (70.0%) and the winter (53.5%), while the autumn represented the lowest rate of infection (52.3%). In addition, this study provides additional morphological characters for the parasite Paracamallanus cyathopharynx based on the scanning electron microscopy.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73754666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
PROTECTIVE EFFECT OF GARLIC OIL AGAINST TARTRAZINE-INDUCED HAEMATO-IMMUNOTOXICITY IN RATS 大蒜油对酒石黄致大鼠血液免疫毒性的保护作用
Pub Date : 2022-09-30 DOI: 10.21608/ejz.2022.147732.1084
Heba M. Abd El Latif, A. El-Morsy
{"title":"PROTECTIVE EFFECT OF GARLIC OIL AGAINST TARTRAZINE-INDUCED HAEMATO-IMMUNOTOXICITY IN RATS","authors":"Heba M. Abd El Latif, A. El-Morsy","doi":"10.21608/ejz.2022.147732.1084","DOIUrl":"https://doi.org/10.21608/ejz.2022.147732.1084","url":null,"abstract":"","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74399672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA LEAVES: IN VITRO AND IN VIVO STUDIES 芦笋叶乙醇提取物的抗氧化和保肝活性:体外和体内研究
Pub Date : 2022-09-29 DOI: 10.21608/ejz.2022.139640.1083
H. Hussien, H. Tag, E. Ahmed, Z. Nabil, M. El-Naggar
{"title":"THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA LEAVES: IN VITRO AND IN VIVO STUDIES","authors":"H. Hussien, H. Tag, E. Ahmed, Z. Nabil, M. El-Naggar","doi":"10.21608/ejz.2022.139640.1083","DOIUrl":"https://doi.org/10.21608/ejz.2022.139640.1083","url":null,"abstract":"","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87627827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PROTECTIVE EFFECT OF AQUEOUS EXTRACT OF MORINGA OLEIFERA LEAVES AGAINST POTASSIUM BROMATE-INDUCED RENAL TOXICITY IN RATS 辣木叶水提物对溴酸钾致大鼠肾毒性的保护作用
Pub Date : 2022-07-12 DOI: 10.21608/ejz.2022.135884.1081
A. Kamel, M. Fouad, Heba Mohamed
KBrO 3 is a vital component used in food, beer, pharmaceutical, and cosmetic productions; it produces moderate-to-dangerous toxic insults to a variety of organs. This study aimed to investigate if Moringa oleifera leaves aqueous extract (MOE) can protect rats from KBrO 3 -induced renal toxicity. Four experimental groups of male albino rats (Sprague Dawley) were used here (n=8): control, MOE (400 mg/kg body weight), KBrO 3 (100 mg/kg body weight), and KBrO 3 in combination with MOE groups. Daily for six weeks, each group received orally its unique treatment. After the experimental period kidneys and serum were collected for biochemical, molecular, and histological investigations. The KBrO 3 treatment was associated with a significant rise in serum levels of urea, creatinine, sodium, and potassium. KBrO 3 also caused a significant increase in the renal tissue levels of malondialdehyde and nitric oxide, while reducing the activities of antioxidant enzymes in the renal tissues. Moreover, KBrO 3 led to kidney inflammation and fibrosis by increasing the tumor necrosis factor-α, interleukin-6, and tumor growth factor-β1, which was followed by upregulation in the renal expression of miRNA 21 ( miR-21 ), miR-29 , and miR-192 . In comparison to the control group, histopathological evaluation of the KBrO 3 group revealed degenerative alterations and damage in the kidney tissues. Conversely, co-treatment with MOE revealed a noticeable alleviation of the harmful effects of KBrO 3 in almost all examined parameters. In conclusion, MOE could be utilized as an alternative therapy to alleviate the detrimental effects of KBrO 3 on kidneys due to its antioxidant, anti-inflammatory, and anti-fibrotic activities.
KBrO 3是食品、啤酒、制药和化妆品生产中使用的重要成分;它对多种器官产生中度到危险的毒性损害。本研究旨在探讨辣木叶水提物(MOE)对kbro3诱导的大鼠肾毒性的保护作用。选用雄性白化大鼠(Sprague Dawley) 4个实验组(n=8):对照组、MOE组(400 mg/kg体重)、kbro3组(100 mg/kg体重)、kbro3与MOE联合组。每天口服,连续6周。实验结束后,收集肾脏和血清进行生化、分子和组织学检查。kbro3治疗与血清尿素、肌酐、钠和钾水平显著升高相关。kbro3还引起肾组织丙二醛和一氧化氮水平的显著升高,同时降低肾组织中抗氧化酶的活性。此外,kbro3通过增加肿瘤坏死因子-α、白细胞介素-6和肿瘤生长因子-β1,导致肾脏炎症和纤维化,随后上调肾脏miRNA 21 (miR-21)、miR-29和miR-192的表达。与对照组相比,kbro3组的组织病理学评估显示肾脏组织出现退行性改变和损伤。相反,与MOE共同处理显示,在几乎所有检查参数中,kbro3的有害影响都明显减轻。综上所述,MOE具有抗氧化、抗炎和抗纤维化的作用,可以作为一种替代疗法来减轻KBrO - 3对肾脏的有害影响。
{"title":"PROTECTIVE EFFECT OF AQUEOUS EXTRACT OF MORINGA OLEIFERA LEAVES AGAINST POTASSIUM BROMATE-INDUCED RENAL TOXICITY IN RATS","authors":"A. Kamel, M. Fouad, Heba Mohamed","doi":"10.21608/ejz.2022.135884.1081","DOIUrl":"https://doi.org/10.21608/ejz.2022.135884.1081","url":null,"abstract":"KBrO 3 is a vital component used in food, beer, pharmaceutical, and cosmetic productions; it produces moderate-to-dangerous toxic insults to a variety of organs. This study aimed to investigate if Moringa oleifera leaves aqueous extract (MOE) can protect rats from KBrO 3 -induced renal toxicity. Four experimental groups of male albino rats (Sprague Dawley) were used here (n=8): control, MOE (400 mg/kg body weight), KBrO 3 (100 mg/kg body weight), and KBrO 3 in combination with MOE groups. Daily for six weeks, each group received orally its unique treatment. After the experimental period kidneys and serum were collected for biochemical, molecular, and histological investigations. The KBrO 3 treatment was associated with a significant rise in serum levels of urea, creatinine, sodium, and potassium. KBrO 3 also caused a significant increase in the renal tissue levels of malondialdehyde and nitric oxide, while reducing the activities of antioxidant enzymes in the renal tissues. Moreover, KBrO 3 led to kidney inflammation and fibrosis by increasing the tumor necrosis factor-α, interleukin-6, and tumor growth factor-β1, which was followed by upregulation in the renal expression of miRNA 21 ( miR-21 ), miR-29 , and miR-192 . In comparison to the control group, histopathological evaluation of the KBrO 3 group revealed degenerative alterations and damage in the kidney tissues. Conversely, co-treatment with MOE revealed a noticeable alleviation of the harmful effects of KBrO 3 in almost all examined parameters. In conclusion, MOE could be utilized as an alternative therapy to alleviate the detrimental effects of KBrO 3 on kidneys due to its antioxidant, anti-inflammatory, and anti-fibrotic activities.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75940368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DIFFERENTIAL STAINING PATTERNS FOR EVALUATION OF OVARIAN AND OVIDUCTS FIBROSIS USING THE POLARIZED LIGHT MICROSCOPE AND THE DIGITAL IMAGES ANALYSIS 鉴别染色模式评估卵巢和输卵管纤维化使用偏光显微镜和数字图像分析
Pub Date : 2022-07-12 DOI: 10.21608/ejz.2022.134076.1080
R. Ahmed
Several histological and immunohistochemical techniques are used in the diagnosis of fibrosis. To better visualization of collagen fibers in both normal and pathological tissues, new optical approaches are required. The present study aimed to investigate/compare the efficiency of various histological stains, using the traditional light microscope and the light polarizing microscope, to quantify/evaluate the ovarian and oviduct fibrosis induced by high-fat diet (HFD) consumption. Twenty-Four female albino rats ( Rattus norvegicus albinus ) were randomly allotted into 4 groups (n=6): control groups (fed on standard diet for 6 and 12 weeks), HFD1 group (fed on HFD for 6 weeks), and HFD2 group (fed on HFD for 12 weeks). The ovarian and oviduct fibrosis were investigated via histological stains including hematoxylin and eosin, Picrosirius Red, van Gieson, and Masson’s trichrome stains using traditional light and polarizing light microscopes, in addition to immunohistochemical demonstrations of α-smooth muscle actin (α-SMA) and vimentin. Hydroxyproline and tumor necrosis factor-α (TNF-α) were also investigated biochemically in the ovarian and oviduct tissues. The results showed that the HFD induced ovarian and oviducts fibrosis in time-dependent manner with significant increases in hydroxyproline and TNF-α values, as well as immunoexpression of α-SMA and vimentin. Picrosirius red stain achieved ideal results in collagen demonstration when compared with van Gieson and Masson’s trichrome stains. Picrosirius red stain in combination with the polarization microscope represented high sensitivity in collagen estimation in comparison with light microscopy. This work recommended using polarizing microscope as a tool to evaluate fibrosis of both the ovaries and the oviducts.
几种组织学和免疫组织化学技术用于纤维化的诊断。为了更好地显示正常和病理组织中的胶原纤维,需要新的光学方法。本研究旨在探讨/比较不同组织学染色方法在传统光学显微镜和光偏光显微镜下对高脂饮食(high-fat diet, HFD)引起的卵巢和输卵管纤维化的定量评价。雌性白化大鼠24只,随机分为4组(n=6):对照组(饲喂标准饲料6周、12周)、HFD1组(饲喂高脂饲料6周)、HFD2组(饲喂高脂饲料12周)。采用苏木精和伊红、Picrosirius Red、van Gieson和Masson’s三色染色,以及α-平滑肌肌动蛋白(α-SMA)和vimentin的免疫组化检测,观察卵巢和输卵管纤维化情况。同时对卵巢和输卵管组织中羟脯氨酸和肿瘤坏死因子-α (TNF-α)进行生化检测。结果表明,HFD诱导卵巢和输卵管纤维化呈时间依赖性,羟脯氨酸和TNF-α值显著升高,α-SMA和vimentin的免疫表达显著升高。与van Gieson和Masson的三色染色相比,小天狼星红染色在胶原蛋白展示方面取得了理想的效果。与光镜相比,小天狼星红染色联合偏光显微镜对胶原蛋白的估计具有较高的灵敏度。本工作推荐使用偏光显微镜作为评估卵巢和输卵管纤维化的工具。
{"title":"DIFFERENTIAL STAINING PATTERNS FOR EVALUATION OF OVARIAN AND OVIDUCTS FIBROSIS USING THE POLARIZED LIGHT MICROSCOPE AND THE DIGITAL IMAGES ANALYSIS","authors":"R. Ahmed","doi":"10.21608/ejz.2022.134076.1080","DOIUrl":"https://doi.org/10.21608/ejz.2022.134076.1080","url":null,"abstract":"Several histological and immunohistochemical techniques are used in the diagnosis of fibrosis. To better visualization of collagen fibers in both normal and pathological tissues, new optical approaches are required. The present study aimed to investigate/compare the efficiency of various histological stains, using the traditional light microscope and the light polarizing microscope, to quantify/evaluate the ovarian and oviduct fibrosis induced by high-fat diet (HFD) consumption. Twenty-Four female albino rats ( Rattus norvegicus albinus ) were randomly allotted into 4 groups (n=6): control groups (fed on standard diet for 6 and 12 weeks), HFD1 group (fed on HFD for 6 weeks), and HFD2 group (fed on HFD for 12 weeks). The ovarian and oviduct fibrosis were investigated via histological stains including hematoxylin and eosin, Picrosirius Red, van Gieson, and Masson’s trichrome stains using traditional light and polarizing light microscopes, in addition to immunohistochemical demonstrations of α-smooth muscle actin (α-SMA) and vimentin. Hydroxyproline and tumor necrosis factor-α (TNF-α) were also investigated biochemically in the ovarian and oviduct tissues. The results showed that the HFD induced ovarian and oviducts fibrosis in time-dependent manner with significant increases in hydroxyproline and TNF-α values, as well as immunoexpression of α-SMA and vimentin. Picrosirius red stain achieved ideal results in collagen demonstration when compared with van Gieson and Masson’s trichrome stains. Picrosirius red stain in combination with the polarization microscope represented high sensitivity in collagen estimation in comparison with light microscopy. This work recommended using polarizing microscope as a tool to evaluate fibrosis of both the ovaries and the oviducts.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74486096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EFFECT OF WATER QUALITY ON THE EFFICIENCY OF COMMIPHORA MOLMOL RESIN, MIRAZID, AND FORMULATED AND CRYSTALLINE ACETYLSALICYLIC ACIDS IN CONTROLLING CULEX PIPIENS 水质对红棉酚树脂、咪唑啉、乙酰水杨酸和结晶水杨酸防治库蚊效果的影响
Pub Date : 2022-07-11 DOI: 10.21608/ejz.2022.136069.1082
Faten Abo El-Dahab, M. Baz, Y. El-Sayed, Rasha Abdel Hameed, Abla Abd El Meguid
The mosquitoes are nuisance pests to humans and represent a global health challenge. The larvicidal activities of Commiphora molmol resin, mirazid, and formulated and crystalline acetylsalicylic acid (F-ASA and C-ASA, respectively) were evaluated against the 2 nd and 4 th instar larvae of Culex pipiens at 27°C, after 48 hours post-treatment in different water qualities. Water samples were collected from the tap, canal, and pool. The water pH, nitrate, salinity, and dissolved oxygen were measured. The results showed that the larvicidal activity of the tested compounds was increased by increasing their concentrations. The mortality reached 100% and 98.4% for the 2 nd instar larvae at 160 ppm, 100% and 90.4% for the 4 th instar larvae at 320 ppm of mirazid and C. molmol , respectively. The mortality induced by F-ASA and C-ASA reached 99.2% and 75.2% for the 2 nd instar larvae at 1200 ppm, 100% and 92.0% for the 4 th instar larvae at 2000 ppm, respectively. The efficiency of the tested materials against the 4 th instar larvae was affected by the water quality; whereas their LC 50 values were 38.3-1045.9 ppm in tap water; while at canal and pool water the LC 50 values were 44.5-1073 and 49.8-1126.0 ppm, respectively, at 27°C and 48 hours post-treatment. The correlation coefficient between the susceptibility of Cx. pipiens larvae to the tested compounds and each physicochemical factor showed a positive correlation with water nitrate and dissolved oxygen. In conclusion, mirazid, C. molmol , F-ASA, and C-ASA can be good alternative agents for controlling mosquitos’ larvae in different water qualities.
蚊子对人类来说是一种讨厌的害虫,对全球健康构成了挑战。在不同的水质条件下,27℃条件下,48 h后,测定了红棉草树脂、米拉齐、乙酰水杨酸和结晶乙酰水杨酸(分别为F-ASA和C- asa)对库蚊2龄和4龄幼虫的杀虫活性。从水龙头、运河和水池中采集水样。测定了水的pH值、硝酸盐、盐度和溶解氧。结果表明,各化合物浓度越高,其杀虫活性越强。在浓度为160 ppm和320 ppm时,2龄幼虫死亡率分别为100%和98.4%,4龄幼虫死亡率分别为100%和90.4%。在1200 ppm浓度下,F-ASA和C-ASA对2龄幼虫的死亡率分别为99.2%和75.2%,在2000 ppm浓度下,对4龄幼虫的死亡率分别为100%和92.0%。试验材料对4龄幼虫的杀灭效果受水质影响;而自来水中的LC 50值为38.3 ~ 1045.9 ppm;处理后27℃和48 h,渠水和池水的LC 50值分别为44.5 ~ 1073和49.8 ~ 1126.0 ppm。对Cx的敏感性进行了相关系数分析。库蚊幼虫对被试化合物及各理化因子均与水体硝酸盐和溶解氧呈正相关。综上所述,mirazid、C. molmol、F-ASA和C-ASA可作为不同水质条件下蚊虫幼虫的较好替代药剂。
{"title":"EFFECT OF WATER QUALITY ON THE EFFICIENCY OF COMMIPHORA MOLMOL RESIN, MIRAZID, AND FORMULATED AND CRYSTALLINE ACETYLSALICYLIC ACIDS IN CONTROLLING CULEX PIPIENS","authors":"Faten Abo El-Dahab, M. Baz, Y. El-Sayed, Rasha Abdel Hameed, Abla Abd El Meguid","doi":"10.21608/ejz.2022.136069.1082","DOIUrl":"https://doi.org/10.21608/ejz.2022.136069.1082","url":null,"abstract":"The mosquitoes are nuisance pests to humans and represent a global health challenge. The larvicidal activities of Commiphora molmol resin, mirazid, and formulated and crystalline acetylsalicylic acid (F-ASA and C-ASA, respectively) were evaluated against the 2 nd and 4 th instar larvae of Culex pipiens at 27°C, after 48 hours post-treatment in different water qualities. Water samples were collected from the tap, canal, and pool. The water pH, nitrate, salinity, and dissolved oxygen were measured. The results showed that the larvicidal activity of the tested compounds was increased by increasing their concentrations. The mortality reached 100% and 98.4% for the 2 nd instar larvae at 160 ppm, 100% and 90.4% for the 4 th instar larvae at 320 ppm of mirazid and C. molmol , respectively. The mortality induced by F-ASA and C-ASA reached 99.2% and 75.2% for the 2 nd instar larvae at 1200 ppm, 100% and 92.0% for the 4 th instar larvae at 2000 ppm, respectively. The efficiency of the tested materials against the 4 th instar larvae was affected by the water quality; whereas their LC 50 values were 38.3-1045.9 ppm in tap water; while at canal and pool water the LC 50 values were 44.5-1073 and 49.8-1126.0 ppm, respectively, at 27°C and 48 hours post-treatment. The correlation coefficient between the susceptibility of Cx. pipiens larvae to the tested compounds and each physicochemical factor showed a positive correlation with water nitrate and dissolved oxygen. In conclusion, mirazid, C. molmol , F-ASA, and C-ASA can be good alternative agents for controlling mosquitos’ larvae in different water qualities.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88755301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
COMPARATIVE BIOACTIVITY OF SILVER, ALUMINUM OXIDE, AND ZINC OXIDE NANOPARTICLES ON THE HOUSE FLY “MUSCA DOMESTICA L. (DIPTERA: MUSCIDAE)” 银、氧化铝和氧化锌纳米颗粒对家蝇的生物活性比较(双翅目:蝇科)
Pub Date : 2022-07-11 DOI: 10.21608/ejz.2022.124472.1075
A. Assar, Shawkia Z. Mahmoud, S. Mahmoud
The aim of the current study was to identify and compare the biological consequences of three nanoparticles (NPs): “silver, aluminum oxide, and zinc oxide” against the house fly “ Musca domestica L.”. The NPs were applied by feeding the early second instar larvae on diets mixed with the selected NPs at varying concentrations (5, 10, 20, 30, 40, and 60 mg/g diet). The consequences demonstrated that all the tested NPs were toxic to M . domestica larvae. Silver NPs were the most toxic, induced 100% larval mortalities at 40 mg/g diet and its toxicity index was 100. Median lethal concentration values (LC 50 ) were 20.8, 38.7, and 49.6 mg/g diet for Ag, Al 2 O 3, and ZnO NPs, respectively. The tested NPs caused a significant prolongation ( P <0.05) in larval and pupal period. The pupation percent and adult emergence decreased significantly ( P <0.05) by all NPs as contrasted to the control group. All the tested NPs caused a reduction in the larval and pupal weights. In addition,
本研究的目的是鉴定和比较三种纳米粒子(“银、氧化铝和氧化锌”)对家蝇的生物学后果。选择不同浓度的NPs(5、10、20、30、40和60 mg/g)饲喂二龄早期幼虫。结果表明,所有检测的NPs对M均有毒性。有幼虫。以银NPs毒性最大,40 mg/g时幼虫死亡率为100%,毒性指数为100。Ag、al2o3和ZnO NPs的中位致死浓度(LC 50)分别为20.8、38.7和49.6 mg/g。NPs显著延长了幼虫期和蛹期(P <0.05)。各NPs组的化蛹率和成虫羽化率均显著低于对照组(P <0.05)。所有被试的NPs都能降低幼虫和蛹的体重。此外,
{"title":"COMPARATIVE BIOACTIVITY OF SILVER, ALUMINUM OXIDE, AND ZINC OXIDE NANOPARTICLES ON THE HOUSE FLY “MUSCA DOMESTICA L. (DIPTERA: MUSCIDAE)”","authors":"A. Assar, Shawkia Z. Mahmoud, S. Mahmoud","doi":"10.21608/ejz.2022.124472.1075","DOIUrl":"https://doi.org/10.21608/ejz.2022.124472.1075","url":null,"abstract":"The aim of the current study was to identify and compare the biological consequences of three nanoparticles (NPs): “silver, aluminum oxide, and zinc oxide” against the house fly “ Musca domestica L.”. The NPs were applied by feeding the early second instar larvae on diets mixed with the selected NPs at varying concentrations (5, 10, 20, 30, 40, and 60 mg/g diet). The consequences demonstrated that all the tested NPs were toxic to M . domestica larvae. Silver NPs were the most toxic, induced 100% larval mortalities at 40 mg/g diet and its toxicity index was 100. Median lethal concentration values (LC 50 ) were 20.8, 38.7, and 49.6 mg/g diet for Ag, Al 2 O 3, and ZnO NPs, respectively. The tested NPs caused a significant prolongation ( P <0.05) in larval and pupal period. The pupation percent and adult emergence decreased significantly ( P <0.05) by all NPs as contrasted to the control group. All the tested NPs caused a reduction in the larval and pupal weights. In addition,","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85976680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Egyptian Journal of Zoology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1