{"title":"HEALTH STATUS OF DATE MUSSEL \"LITHOPHAGA LITHOPHAGA, FAMILY: MYTILIDAE\" IN ALEXANDERIA, EGYPT: POTENTIAL EFFECTS OF THE HEAVY METAL POLLUTION","authors":"W. Salama, M. Mona, Aml Ghoneium","doi":"10.21608/EJZ.2020.25041.1022","DOIUrl":"https://doi.org/10.21608/EJZ.2020.25041.1022","url":null,"abstract":"Article History: Received: 29 March 2020 Revised: 9 April 2020 Accepted: 10 April 2020 Published Online: 22 April 2020","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"62 1","pages":"26-38"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84071484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/EJZ.2020.29834.1031
Samah M. Arisha, S. A. Saker, Fatma R. Abd El-Haseeb
High fat diet (HFD) induces hyperlipidemia and causes non-alcoholic fatty liver disease. Cinnamon (Cinnamomum zeylanicum) has many curative effects as anti-inflammatory and antioxidant. This study aimed to investigate the potential protective effect of cinnamon against HFD-induced dyslipidemia and liver steatosis. Forty adult male Wistar albino rats were used in the current study; these were allotted randomly to 4 equal groups. The animals were fed on standard diet (group 1), standard diet containing cinnamon powder “15% weight by weight, w/w” (group 2), HFD (group 3), or HFD containing cinnamon powder “15% w/w” (group 4) daily for 4 or 8 weeks. The results showed that HFD increased significantly (P≤0.05) the body weight and the activities of serum aminotransferases, and induced dyslipidemia. Examination of the liver of the HFD-fed rats showed many pathological changes, especially intracytoplasmic fat accumulation either as small lipid droplets with variable sizes (microsteatosis) or large lipid droplets (macrostatosis) that may displace the nucleus laterally. Ultrastructural examination of the same group showed various degenerative alterations in the nucleus, mitochondria, endoplasmic reticulum, and Kupffer cells, as well as lipid accumulation. However, examination of liver of animals fed on HFD+cinnamon showed a remarkable improvement especially after 8 weeks; the histological and ultrastructure pictures were mostly similar to the control group. In addition, the animals fed on HFD+cinnamon recorded a decrease in the body weight and improvement in readings of the biochemical parameters compared with the HFD-treated group. In conclusion, cinnamon alleviated the occurrence of non-alcoholic fatty liver disease in the HFD-fed rats.
{"title":"CINNAMON REDUCES DYSLIPIDEMIA AND LIVER STEATOSIS INDUCED BY HIGH FAT DIET IN ALBINO RATS: HISTOLOGICAL, ULTRASTRUCTURAL, AND BIOCHEMICAL STUDIES","authors":"Samah M. Arisha, S. A. Saker, Fatma R. Abd El-Haseeb","doi":"10.21608/EJZ.2020.29834.1031","DOIUrl":"https://doi.org/10.21608/EJZ.2020.29834.1031","url":null,"abstract":"High fat diet (HFD) induces hyperlipidemia and causes non-alcoholic fatty liver disease. Cinnamon (Cinnamomum zeylanicum) has many curative effects as anti-inflammatory and antioxidant. This study aimed to investigate the potential protective effect of cinnamon against HFD-induced dyslipidemia and liver steatosis. Forty adult male Wistar albino rats were used in the current study; these were allotted randomly to 4 equal groups. The animals were fed on standard diet (group 1), standard diet containing cinnamon powder “15% weight by weight, w/w” (group 2), HFD (group 3), or HFD containing cinnamon powder “15% w/w” (group 4) daily for 4 or 8 weeks. The results showed that HFD increased significantly (P≤0.05) the body weight and the activities of serum aminotransferases, and induced dyslipidemia. Examination of the liver of the HFD-fed rats showed many pathological changes, especially intracytoplasmic fat accumulation either as small lipid droplets with variable sizes (microsteatosis) or large lipid droplets (macrostatosis) that may displace the nucleus laterally. Ultrastructural examination of the same group showed various degenerative alterations in the nucleus, mitochondria, endoplasmic reticulum, and Kupffer cells, as well as lipid accumulation. However, examination of liver of animals fed on HFD+cinnamon showed a remarkable improvement especially after 8 weeks; the histological and ultrastructure pictures were mostly similar to the control group. In addition, the animals fed on HFD+cinnamon recorded a decrease in the body weight and improvement in readings of the biochemical parameters compared with the HFD-treated group. In conclusion, cinnamon alleviated the occurrence of non-alcoholic fatty liver disease in the HFD-fed rats.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85537471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/EJZ.2020.24247.1021
Marwa Atwa, Noha Salem, H. Mohamed
There are few data regarding the genotoxic and immunomodulatory effects of Cryptosporidium parvum (Family: Cryptosporidiidae) infection in vivo. Therefore, the present study evaluated the effect of Cryptosporidium parvum infection on DNA and gene expressions of interleukin-6 (Il-6) and tumor necrosis factor-α (Tnf-α) in blood of the immunocompromised mice. Fifty adult male Swiss albino mice were used in the present study; these were randomly divided into five groups (10 mice/each group) as follows: group I served as a healthy, uninfected control group; groups II-IV were immunosuppressed by dexamethasone, and uninfected, or infected (orally) with 3.0×103 (low dose) or 1.0×104 (high dose) Cryptosporidium parvum oocysts, respectively; group V did not receive dexamethasone and infected with 1.0×104 Cryptosporidium parvum oocysts. DNA damage of leucocytes was assessed by flow cytometry and comet assay; the gene expression of the cytokines was performed using real-time polymerase chain reaction. The results revealed that infection with the low or the high dose of Cryptosporidium parvum induced DNA damage in the leucocytes of the immunosuppressed mice, as revealed by the elevations in tail length and tail moment of the comet assay compared with the healthy and immunosuppressed, uninfected, mice. In addition, significant decreases and increases in the expression of Il-6 and Tnf-α genes, respectively, were recorded in leucocytes of the immunosuppressed, Cryptosporidium parvum-infected mice in comparison with the healthy and immunosuppressed, uninfected, mice. In conclusion, Cryptosporidium infection induced DNA damage and augmented the changes induced in the Il-6and Tnf-α gene expressions in the leucocytes of the immunosuppressed mice.
{"title":"EFFECT OF CRYPTOSPORIDIUM PARVUM INFECTION ON DNA DAMAGE AND CYTOKINES GENE EXPRESSION IN BLOOD OF IMMUNOCOMPROMISED MICE","authors":"Marwa Atwa, Noha Salem, H. Mohamed","doi":"10.21608/EJZ.2020.24247.1021","DOIUrl":"https://doi.org/10.21608/EJZ.2020.24247.1021","url":null,"abstract":"There are few data regarding the genotoxic and immunomodulatory effects of Cryptosporidium parvum (Family: Cryptosporidiidae) infection in vivo. Therefore, the present study evaluated the effect of Cryptosporidium parvum infection on DNA and gene expressions of interleukin-6 (Il-6) and tumor necrosis factor-α (Tnf-α) in blood of the immunocompromised mice. Fifty adult male Swiss albino mice were used in the present study; these were randomly divided into five groups (10 mice/each group) as follows: group I served as a healthy, uninfected control group; groups II-IV were immunosuppressed by dexamethasone, and uninfected, or infected (orally) with 3.0×103 (low dose) or 1.0×104 (high dose) Cryptosporidium parvum oocysts, respectively; group V did not receive dexamethasone and infected with 1.0×104 Cryptosporidium parvum oocysts. DNA damage of leucocytes was assessed by flow cytometry and comet assay; the gene expression of the cytokines was performed using real-time polymerase chain reaction. The results revealed that infection with the low or the high dose of Cryptosporidium parvum induced DNA damage in the leucocytes of the immunosuppressed mice, as revealed by the elevations in tail length and tail moment of the comet assay compared with the healthy and immunosuppressed, uninfected, mice. In addition, significant decreases and increases in the expression of Il-6 and Tnf-α genes, respectively, were recorded in leucocytes of the immunosuppressed, Cryptosporidium parvum-infected mice in comparison with the healthy and immunosuppressed, uninfected, mice. In conclusion, Cryptosporidium infection induced DNA damage and augmented the changes induced in the Il-6and Tnf-α gene expressions in the leucocytes of the immunosuppressed mice.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"38 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76062919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/EJZ.2020.26416.1025
E. El-Hussieny, Marwa M. Matoug, W. El-Sayed
General exposure to mercury is conceivable since it has been shown to be exacerbated through contaminated water and food. The present study aimed to evaluate three different flavonoids and selenite against mercuric chloride (HgCl2) toxicity in male Wistar rats. The rats were randomly divided into ten groups (n=7) as follows: a control group, groups orally administered with 5 mg/kg body weight of either sodium selenite or HgCl2, groups orally treated with 50 mg/kg of body weight morin, naringin, or hesperetin, and groups that were orally co-administered with HgCl2 and sodium selenite, morin, naringin, or hesperetin. All treatments continued daily for two weeks. HgCl2 toxicity caused significant elevations in the levels/activities of serum total proteins, globulins, total cholesterol, triacylglycerols, tumor necrosis factor-α, interleukin-6, alanine aminotransferase, and γ-glutamyl transpeptidase, as well as hepatic malondialdehyde and catalase. It also caused significant reductions in the hepatic content of reduced glutathione, as well as hemoglobin content and erythrocytes count. Most of these deleterious effects were ameliorated by the concomitant administration of flavonoids or selenite. There was no structure-activity relationship that could be withdrawn from this study. Naringin with the highest number of hydroxyl groups on B-ring and the highest absolute number of hydroxyl groups in general was, with few exceptions, as efficient as the other flavonoids and selenite as well. Every flavonoid had its own biological signature probably due to its metabolism.
{"title":"THE ATTENUATION OF MERCURIC CHLORIDE TOXICITY BY FLAVONOIDS IN MALE ALBINO RATS IS INDEPENDENT ON THE NUMBER OF HYDROXYL GROUPS ON B-RINGS","authors":"E. El-Hussieny, Marwa M. Matoug, W. El-Sayed","doi":"10.21608/EJZ.2020.26416.1025","DOIUrl":"https://doi.org/10.21608/EJZ.2020.26416.1025","url":null,"abstract":"General exposure to mercury is conceivable since it has been shown to be exacerbated through contaminated water and food. The present study aimed to evaluate three different flavonoids and selenite against mercuric chloride (HgCl2) toxicity in male Wistar rats. The rats were randomly divided into ten groups (n=7) as follows: a control group, groups orally administered with 5 mg/kg body weight of either sodium selenite or HgCl2, groups orally treated with 50 mg/kg of body weight morin, naringin, or hesperetin, and groups that were orally co-administered with HgCl2 and sodium selenite, morin, naringin, or hesperetin. All treatments continued daily for two weeks. HgCl2 toxicity caused significant elevations in the levels/activities of serum total proteins, globulins, total cholesterol, triacylglycerols, tumor necrosis factor-α, interleukin-6, alanine aminotransferase, and γ-glutamyl transpeptidase, as well as hepatic malondialdehyde and catalase. It also caused significant reductions in the hepatic content of reduced glutathione, as well as hemoglobin content and erythrocytes count. Most of these deleterious effects were ameliorated by the concomitant administration of flavonoids or selenite. There was no structure-activity relationship that could be withdrawn from this study. Naringin with the highest number of hydroxyl groups on B-ring and the highest absolute number of hydroxyl groups in general was, with few exceptions, as efficient as the other flavonoids and selenite as well. Every flavonoid had its own biological signature probably due to its metabolism.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87527938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-01DOI: 10.12816/EJZ.2019.18937.1017
S. Fathy, Noha I Said
The current study aimed to investigate the potential immunomodulatory and antigenotoxic impacts of propolis in paclitaxel (PTX)-treated rats. Twenty four male Sprague Dawley rats were used in the present study and randomly/equally divided into four groups; control group, PTX group that was intraperitoneally injected with 5 mg/kg PTX (once/week), propolis group that received orally/daily 50 mg/kg propolis, and the last group received both PTX and propolis. All treatments were given for four weeks. The results showed a significant upregulation in the nuclear factor kappa B (Nf-κb) gene expression in the spleen of PTX group, as compared with the control group. In addition, substantial increases in tumor necrosis factor-α, interferon-γ, C-X-C motif ligand 10, CC motif ligand 2, and interleukin (IL)-4 concentrations, while a significant reduction in IL-10 concentration, were detected in the spleen of PTX group compared with the control group. Moreover, PTX resulted in a significant elevation in the frequency of micronucleated polychromatic erythrocytes in bone marrow cells compared with the control group. On the other hand, the treatment with propolis alone did not significantly affect all of these parameters, as compared with the control group. However, propolis decreased significantly all recorded side effects in the PTX-treated rats. In conclusion, propolis can be used as an adjunct with PTX to modulate the cytokines and chemokines release of splenic immune cells, as well as to counteract the genotoxic effect of PTX on bone marrow cells, through downregulating the splenic Nf-κb gene expression, and reducing the bone marrow micronuclei formation, respectively
本研究旨在探讨蜂胶对紫杉醇(PTX)治疗大鼠的潜在免疫调节和抗毒素作用。本研究选用24只雄性Sprague Dawley大鼠，随机/平均分为4组;对照组，PTX组腹腔注射PTX 5 mg/kg(1次/周)，蜂胶组口服蜂胶50 mg/kg /d，最后一组同时注射PTX和蜂胶。所有治疗疗程均为四周。结果显示，与对照组相比，PTX组脾核因子κ B (Nf-κb)基因表达明显上调。此外，与对照组相比，PTX组脾脏肿瘤坏死因子-α、干扰素-γ、C-X-C基序配体10、CC基序配体2和白细胞介素(IL)-4浓度显著升高，IL-10浓度显著降低。此外，与对照组相比，PTX导致骨髓细胞中微核多染红细胞的频率显著升高。另一方面，与对照组相比，单独使用蜂胶处理对所有这些参数没有显著影响。然而，蜂胶显著降低了ptx治疗大鼠所有记录的副作用。综上所述，蜂胶可作为PTX的佐剂，通过下调脾脏Nf-κb基因的表达，减少骨髓微核的形成，调节脾脏免疫细胞的细胞因子和趋化因子的释放，抵消PTX对骨髓细胞的遗传毒性作用
{"title":"IMMUNOMODULATION AND ANTIGENOTOXIC EFFECTS OF PROPOLIS IN PACLITAXEL-TREATED RATS","authors":"S. Fathy, Noha I Said","doi":"10.12816/EJZ.2019.18937.1017","DOIUrl":"https://doi.org/10.12816/EJZ.2019.18937.1017","url":null,"abstract":"The current study aimed to investigate the potential immunomodulatory and antigenotoxic impacts of propolis in paclitaxel (PTX)-treated rats. Twenty four male Sprague Dawley rats were used in the present study and randomly/equally divided into four groups; control group, PTX group that was intraperitoneally injected with 5 mg/kg PTX (once/week), propolis group that received orally/daily 50 mg/kg propolis, and the last group received both PTX and propolis. All treatments were given for four weeks. The results showed a significant upregulation in the nuclear factor kappa B (Nf-κb) gene expression in the spleen of PTX group, as compared with the control group. In addition, substantial increases in tumor necrosis factor-α, interferon-γ, C-X-C motif ligand 10, CC motif ligand 2, and interleukin (IL)-4 concentrations, while a significant reduction in IL-10 concentration, were detected in the spleen of PTX group compared with the control group. Moreover, PTX resulted in a significant elevation in the frequency of micronucleated polychromatic erythrocytes in bone marrow cells compared with the control group. On the other hand, the treatment with propolis alone did not significantly affect all of these parameters, as compared with the control group. However, propolis decreased significantly all recorded side effects in the PTX-treated rats. In conclusion, propolis can be used as an adjunct with PTX to modulate the cytokines and chemokines release of splenic immune cells, as well as to counteract the genotoxic effect of PTX on bone marrow cells, through downregulating the splenic Nf-κb gene expression, and reducing the bone marrow micronuclei formation, respectively","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86771149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-01DOI: 10.12816/EJZ.2019.19091.1018
Hewaydah E. Abou Shafeey
Microtetrameres spiralis is one of the common spirurid parasites recovered from the cattle egret “Ardeola ibis ibis”. No data was available on its ultrastructural characteristics with scanning electron microscopy. Therefore, the present study aimed to investigate, in details, the characteristic features of M. spiralis in cattle egret using both the light and scanning electron microscopies. The results revealed that nine out of seventeen Ardeola ibis ibis (53%) were infected with M. spiralis. Female worms were deeply embedded in proventriculus wall; their bodies were twisted in longitudinal tight spiral forming 2-3 turns. Males move in lumen; sometimes they were lodged on lining. They have filiform cylindrical shape. Male possesses two unequal and dissimilar spicules. The buccal capsule is well-chitinized, thick-walled, flask-shaped in females, but tubular in males. The vulva lies at the posterior third of the body. Cuticle was sharp transverse annulated along whole length of the body without alae or spines. Each pseudolabium has inner and outer edges; the outer edge has wavy-shaped, triple swollen processes. The depressed part that located between the two edges of each pseudolabium contained three corrugated, flower-shaped processes. The anterior annulations are ridged and have numerous pointed triangular processes. A single raised corrugated papilla appeared. Button-like structures are distributed on longitudinal striations. An oval striated, papilla-shaped structure is elevated with coiled, rod-shaped projection. The transverse annulations over the mid-portion of the female’s body have wavy margins.
{"title":"SCANNING ELECTRON MICROSCOPY OF MICROTETRAMERES SPIRALIS (NEMATODA, FAMILY TETRAMERIDAE) PARASITISING CATTLE EGRET “ARDEOLA IBIS IBIS” IN EGYPT","authors":"Hewaydah E. Abou Shafeey","doi":"10.12816/EJZ.2019.19091.1018","DOIUrl":"https://doi.org/10.12816/EJZ.2019.19091.1018","url":null,"abstract":"Microtetrameres spiralis is one of the common spirurid parasites recovered from the cattle egret “Ardeola ibis ibis”. No data was available on its ultrastructural characteristics with scanning electron microscopy. Therefore, the present study aimed to investigate, in details, the characteristic features of M. spiralis in cattle egret using both the light and scanning electron microscopies. The results revealed that nine out of seventeen Ardeola ibis ibis (53%) were infected with M. spiralis. Female worms were deeply embedded in proventriculus wall; their bodies were twisted in longitudinal tight spiral forming 2-3 turns. Males move in lumen; sometimes they were lodged on lining. They have filiform cylindrical shape. Male possesses two unequal and dissimilar spicules. The buccal capsule is well-chitinized, thick-walled, flask-shaped in females, but tubular in males. The vulva lies at the posterior third of the body. Cuticle was sharp transverse annulated along whole length of the body without alae or spines. Each pseudolabium has inner and outer edges; the outer edge has wavy-shaped, triple swollen processes. The depressed part that located between the two edges of each pseudolabium contained three corrugated, flower-shaped processes. The anterior annulations are ridged and have numerous pointed triangular processes. A single raised corrugated papilla appeared. Button-like structures are distributed on longitudinal striations. An oval striated, papilla-shaped structure is elevated with coiled, rod-shaped projection. The transverse annulations over the mid-portion of the female’s body have wavy margins.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"23 1","pages":"57-67"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81719024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-01DOI: 10.12816/EJZ.2019.17844.1014
S. Sabry, Samia M Sakr
The present study investigated the probable protective role of vitamin C in alleviating 5-fluorouracil (5-Fu)-induced nephrotoxicity in male mice (Mus musculus). Thirty male CD-1 mice were equally divided into three groups: Group I (the control group), received 0.9% NaCl; group II, received intraperitoneally (i.p.) 80 mg of 5-Fu/kg body weight (b.wt)/day for four weeks; group III, received (i.p.) 80 mg of 5-Fu plus 12 mg of vitamin C/kg b.wt/day for four weeks. Animals of all groups were killed at the end of the experiment, and the renal tissue samples were taken and processed for light and electron microscopical examinations. Light microscopic observations revealed that administration of 5-Fu caused erosion of the parietal cells of Bowman's capsules, widening of the urinary spaces, and disruption of the glomerular capillaries and hemorrhage. The cells of the proximal and distal tubules exhibited vacuolar degeneration and coagulative necrosis. The nuclei of these cells manifested pyknosis and karyolysis. Ultrastructural examination revealed damage of the parietal epithelium of Bowman's capsules with fusion and destruction of the foot processes of the podocytes. The cells of the proximal tubules displayed destruction of the microvilli constituting the brush borders and degeneration of the mitochondria. The distal tubules displayed destruction of both the basal infoldings and the mitochondria with fragmentation of the rough endoplasmic reticulum. Histological and ultrastructural results revealed that the treatment with vitamin C simultaneously with 5-Fu led to apparent protection of the renal tissue. This might suggest that vitamin C has a protective potential against 5-Fu-induced nephrotoxicity.
{"title":"NEPHROTOXICITY OF 5-FLUOROURACIL IN MALE MICE AND THE PROBABLE PROTECTIVE ROLE OF VITAMIN C: HISTOLOGICAL AND ULTRASTRUCTURAL STUDIES","authors":"S. Sabry, Samia M Sakr","doi":"10.12816/EJZ.2019.17844.1014","DOIUrl":"https://doi.org/10.12816/EJZ.2019.17844.1014","url":null,"abstract":"The present study investigated the probable protective role of vitamin C in alleviating 5-fluorouracil (5-Fu)-induced nephrotoxicity in male mice (Mus musculus). Thirty male CD-1 mice were equally divided into three groups: Group I (the control group), received 0.9% NaCl; group II, received intraperitoneally (i.p.) 80 mg of 5-Fu/kg body weight (b.wt)/day for four weeks; group III, received (i.p.) 80 mg of 5-Fu plus 12 mg of vitamin C/kg b.wt/day for four weeks. Animals of all groups were killed at the end of the experiment, and the renal tissue samples were taken and processed for light and electron microscopical examinations. Light microscopic observations revealed that administration of 5-Fu caused erosion of the parietal cells of Bowman's capsules, widening of the urinary spaces, and disruption of the glomerular capillaries and hemorrhage. The cells of the proximal and distal tubules exhibited vacuolar degeneration and coagulative necrosis. The nuclei of these cells manifested pyknosis and karyolysis. Ultrastructural examination revealed damage of the parietal epithelium of Bowman's capsules with fusion and destruction of the foot processes of the podocytes. The cells of the proximal tubules displayed destruction of the microvilli constituting the brush borders and degeneration of the mitochondria. The distal tubules displayed destruction of both the basal infoldings and the mitochondria with fragmentation of the rough endoplasmic reticulum. Histological and ultrastructural results revealed that the treatment with vitamin C simultaneously with 5-Fu led to apparent protection of the renal tissue. This might suggest that vitamin C has a protective potential against 5-Fu-induced nephrotoxicity.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87068009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-01DOI: 10.21608/ejz.2019.19091.1018
Hewaydah Abou Shafeey
{"title":"SCANNING ELECTRON MICROSCOPY OF MICROTETRAMERES SPIRALIS (NEMATODA, FAMILY TETRAMERIDAE) PARASITISING CATTLE EGRET “ARDEOLA IBIS IBIS” IN EGYPT","authors":"Hewaydah Abou Shafeey","doi":"10.21608/ejz.2019.19091.1018","DOIUrl":"https://doi.org/10.21608/ejz.2019.19091.1018","url":null,"abstract":"","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73846853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-01DOI: 10.12816/EJZ.2019.18363.1015
R. Mansour
Four adult specimens of clinostomatid parasites were recovered from the buccal cavity and oesophagus of a naturally-infected male squacco heron, Ardeola ralloides (Aves: Ardeidae), collected in July 2019 from Giza Province, Egypt. The rate of natural infection was recorded as 10% (1/10). The parasite has the typical body plan organization of Euclinostomum heterostomum Rudolphi, 1809 (Digenea: Clinostomatidae) with minor differences in certain organs measurements. The body dimensions measure 5.57-6.71 × 2.29-2.97 mm (length × maximum body width). The oral sucker locates in the centre of the oral collar and measures 0.36-0.44 × 0.53-0.64 mm, while the ventral sucker locates in the anterior third of body and measures 0.65-1.10 × 0.63-1.27 mm. The right intestinal caecum gives rise to 11-14 diverticula, while the left intestinal caecum gives rise to 12-15 diverticula. The testes are tandem and lie in the posterior third of the body. The anterior testis is U-shaped and measures 0.61-0.70 × 0.85-1.26 mm, while the posterior testis is tripartite and measures 0.60-0.67 × 0.66-0.69 mm. The ovary is intertesticular, dextral to median body plan, and measures 0.22-0.25 × 0.33-0.37 mm. The uterus is pretesticular, intercaecal, filled with hundreds of eggs, and measures 1.54-2.13 × 0.59-1.32 mm. The uterine eggs are ellipsoidal; each measures 0.11-0.12 × 0.06-0.08 mm. The excretory pore is subterminal, intercaecal, and measures 0.12-0.14 × 0.12-0.14 mm. In addition, the surface topographies of the ventral and the dorsal surfaces of body were described.
{"title":"FIRST RECORD OF EUCLINOSTOMUM HETEROSTOMUM FROM THE NATURALLY-INFECTED HERON “ARDEOLA RALLOIDES” IN EGYPT: A LIGHT & SCANNING ELECTRON MICROSCOPY STUDY","authors":"R. Mansour","doi":"10.12816/EJZ.2019.18363.1015","DOIUrl":"https://doi.org/10.12816/EJZ.2019.18363.1015","url":null,"abstract":"Four adult specimens of clinostomatid parasites were recovered from the buccal cavity and oesophagus of a naturally-infected male squacco heron, Ardeola ralloides (Aves: Ardeidae), collected in July 2019 from Giza Province, Egypt. The rate of natural infection was recorded as 10% (1/10). The parasite has the typical body plan organization of Euclinostomum heterostomum Rudolphi, 1809 (Digenea: Clinostomatidae) with minor differences in certain organs measurements. The body dimensions measure 5.57-6.71 × 2.29-2.97 mm (length × maximum body width). The oral sucker locates in the centre of the oral collar and measures 0.36-0.44 × 0.53-0.64 mm, while the ventral sucker locates in the anterior third of body and measures 0.65-1.10 × 0.63-1.27 mm. The right intestinal caecum gives rise to 11-14 diverticula, while the left intestinal caecum gives rise to 12-15 diverticula. The testes are tandem and lie in the posterior third of the body. The anterior testis is U-shaped and measures 0.61-0.70 × 0.85-1.26 mm, while the posterior testis is tripartite and measures 0.60-0.67 × 0.66-0.69 mm. The ovary is intertesticular, dextral to median body plan, and measures 0.22-0.25 × 0.33-0.37 mm. The uterus is pretesticular, intercaecal, filled with hundreds of eggs, and measures 1.54-2.13 × 0.59-1.32 mm. The uterine eggs are ellipsoidal; each measures 0.11-0.12 × 0.06-0.08 mm. The excretory pore is subterminal, intercaecal, and measures 0.12-0.14 × 0.12-0.14 mm. In addition, the surface topographies of the ventral and the dorsal surfaces of body were described.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"120 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76007257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-02DOI: 10.12816/EJZ.2019.13399.1009
K. Shalloof, A. El-Aiatt, Saber Mohammed
The present study investigated the biological aspects of the European sea bass (Dicentrarchus labrax) from Bardawil Lagoon, North Sinai, Egypt. Monthly random samples of D. labrax were collected from the commercial catch of different landing sites of lagoon during two fishing seasons from May 2015 to December 2016. The length-weight relationship, condition factor, age composition, and fish growth were studied in the current study. The exponent ‘‘b” of the length-weight relationship resulted in isometric mode of growth, since the value of b = 3.0067 in the power equation: Weight = 0.0093 × length3.0067. The mean highest values of condition factor (K) of D. labrax were recorded in November (where K = 1.26). Growth parameters such as the asymptotic length (L∞); growth rate (k); the hypothetical age at which fish would have zero length (t0), and the asymptotic body weight (W∞) were estimated as 75.31 cm, 0.1221 year−1, −1.8703 year and 4088.99 g, respectively. Total mortality (Z), natural mortality (M), and fishing mortality (F) were 0.8786, 0.3153, and 0.5633 year−1,respectively. Exploitation rate (E) was 0.64, which indicated that the stock of the sea bass in the research area is heavily exploited. However, degree of well-being of D. labrax in the lagoon was detected. Overall, the present study concluded that it is essential to maximize the length at the first capture to be larger than that at the first sexual maturity (> 30.0 cm) by widening the mesh size used to catch D. labrax to protect this species from extra-exploitation.
{"title":"BIOLOGICAL ASPECTS OF THE EUROPEAN SEA BASS (DICENTRARCHUS LABRAX L., 1758) FROM BARDAWIL LAGOON, NORTH SINAI, EGYPT","authors":"K. Shalloof, A. El-Aiatt, Saber Mohammed","doi":"10.12816/EJZ.2019.13399.1009","DOIUrl":"https://doi.org/10.12816/EJZ.2019.13399.1009","url":null,"abstract":"The present study investigated the biological aspects of the European sea bass (Dicentrarchus labrax) from Bardawil Lagoon, North Sinai, Egypt. Monthly random samples of D. labrax were collected from the commercial catch of different landing sites of lagoon during two fishing seasons from May 2015 to December 2016. The length-weight relationship, condition factor, age composition, and fish growth were studied in the current study. The exponent ‘‘b” of the length-weight relationship resulted in isometric mode of growth, since the value of b = 3.0067 in the power equation: Weight = 0.0093 × length3.0067. The mean highest values of condition factor (K) of D. labrax were recorded in November (where K = 1.26). Growth parameters such as the asymptotic length (L∞); growth rate (k); the hypothetical age at which fish would have zero length (t0), and the asymptotic body weight (W∞) were estimated as 75.31 cm, 0.1221 year−1, −1.8703 year and 4088.99 g, respectively. Total mortality (Z), natural mortality (M), and fishing mortality (F) were 0.8786, 0.3153, and 0.5633 year−1,respectively. Exploitation rate (E) was 0.64, which indicated that the stock of the sea bass in the research area is heavily exploited. However, degree of well-being of D. labrax in the lagoon was detected. Overall, the present study concluded that it is essential to maximize the length at the first capture to be larger than that at the first sexual maturity (> 30.0 cm) by widening the mesh size used to catch D. labrax to protect this species from extra-exploitation.","PeriodicalId":11659,"journal":{"name":"Egyptian Journal of Zoology","volume":"131 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75820101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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