Environmental Technology & Innovation最新文献_第5页

Antibacterial and biofilm inhibition of Helicobacter pylori using green synthesized MWCNTs/ZnO/Chitosan nanocomposites

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-11 DOI: 10.1016/j.eti.2025.104068

Saeid Fallahizadeh , Mahmood Yousefi , Ahmad Ghasemi , Seyed Abdolmohammad Sadat , Mahnaz Mohtashemi , Alieh Rezagholizade-shirvan , Mohsen Naghmachi

Helicobacter pylori, a Gram-negative bacterium, is a major cause of gastritis, peptic ulcers and gastric cancer. Its biofilm formation and antibiotic resistance in patients make it difficult for treatment. This study concerned the synthesis and antibacterial and antibiofilm evaluation of MWCNTs/ZnO/Chitosan nanocomposite against H. pylori. Green synthesis methods were used to develop the nanocomposite based on the formation of ZnO nanoparticles and the functionalization of MWCNTs. The XRD, SEM, TEM, and FTIR characteristics revealed the structural stability and the successful integration of ZnO, MWCNTs, and chitosan into the composite. The results indicated that the MIC values for ZnO nanoparticles ranged from 25 to 50 μg/mL, while those for chitosan were above 100 μg/mL. Additionally, the MWCNTs/ZnO/Chitosan nanocomposite exhibited the lowest MIC values, with 12.5 μg/mL for H. pylori clinical isolate 1 and 25 μg/mL for H. pylori clinical isolate 2 and H. pylori ATCC 43504 strains. MIC tests showed that the nanocomposite had better antibacterial activity, therefore having lower MIC values than normal antibiotics like metronidazole (MNZ) and clarithromycin (CLR) as well as its components. ZnO-induced reactive oxygen species (ROS), chitosan's bacterial membrane interaction, and MWCNTs part in improved nanoparticle distribution and mechanical biofilm disturbance all contributed to the antimicrobial mechanisms. The research points out the MWCNTs/ZnO/Chitosan nanocomposite as a hopeful solution for antibiotic-resistant H. pylori bacteria causes further in vivo experiments and medical uses rest on its synergistic antibacterial activity and ability to disrupt biofilms. These results emphasize the promise of nanotechnology in creating novel therapies to fight ongoing bacterial infections.

{"title":"Antibacterial and biofilm inhibition of Helicobacter pylori using green synthesized MWCNTs/ZnO/Chitosan nanocomposites","authors":"Saeid Fallahizadeh , Mahmood Yousefi , Ahmad Ghasemi , Seyed Abdolmohammad Sadat , Mahnaz Mohtashemi , Alieh Rezagholizade-shirvan , Mohsen Naghmachi","doi":"10.1016/j.eti.2025.104068","DOIUrl":"10.1016/j.eti.2025.104068","url":null,"abstract":"<div><div><em>Helicobacter pylori</em>, a Gram-negative bacterium, is a major cause of gastritis, peptic ulcers and gastric cancer. Its biofilm formation and antibiotic resistance in patients make it difficult for treatment. This study concerned the synthesis and antibacterial and antibiofilm evaluationof MWCNTs/ZnO/Chitosan nanocomposite against <em>H. pylori.</em> Green synthesis methods were used todevelop the nanocomposite based on the formation of ZnO nanoparticles and the functionalization of MWCNTs. The XRD, SEM, TEM, and FTIR characteristicsrevealed the structural stability and the successful integration of ZnO, MWCNTs, and chitosan into the composite. The results indicated that the MIC values for ZnO nanoparticles ranged from 25 to 50 μg/mL, while those for chitosan were above 100 μg/mL. Additionally, the MWCNTs/ZnO/Chitosan nanocomposite exhibited the lowest MIC values, with 12.5 μg/mL for <em>H. pylori</em> clinical isolate 1 and 25 μg/mL for <em>H. pylori</em> clinical isolate 2 and <em>H. pylori</em> ATCC 43504 strains. MIC tests showed that the nanocomposite had better antibacterial activity, therefore having lower MIC values than normal antibiotics like metronidazole (MNZ) and clarithromycin (CLR) as well as its components. ZnO-induced reactive oxygen species (ROS), chitosan's bacterial membrane interaction, and MWCNTs part in improved nanoparticle distribution and mechanical biofilm disturbance all contributed to the antimicrobial mechanisms. The research points out the MWCNTs/ZnO/Chitosan nanocomposite as a hopeful solution for antibiotic-resistant <em>H. pylori</em> bacteria causes further in vivo experiments and medical uses rest on its synergistic antibacterial activity and ability to disrupt biofilms. These results emphasize the promise of nanotechnology in creating novel therapies to fight ongoing bacterial infections.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104068"},"PeriodicalIF":6.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile synthesis of carbon nitride nanotube confined nano Fe0 for boosting activation of peroxymonosulfate towards tetracycline removal

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-09 DOI: 10.1016/j.eti.2025.104079

Yajun Ji , Feiya Xu , Kun Fang , Huiyun Liu , Xiaofang Pan , Zihe Jin , Lingyun Zheng , Lele Wang

The nano-sized zero-valent iron (Fe⁰) exhibits excellent activity for organic contaminant remediation by activating peroxymonosulfate (PMS). Its catalytic performance, however, was restricted due to its susceptibility towards oxidation and agglomeration. Thus, carbon nitride nanotube embedded nano Fe⁰ catalysts (Fe⁰@NC) with various Fe contents were synthesized to simultaneously overcome the drawbacks. Fe⁰@NC-10 showed high specific surface area (S_BET, 118.87 m²·g⁻¹), stable crystal structure, plentiful Fe- and N-containing active sites. Under the optimal conditions (0.05 g·L⁻¹ Fe⁰@NC-10 and 0.15 g·L⁻¹ PMS), over 86 % tetracycline (TC) could be removed after 5 min, possessing a rate constant (K_obs) value as high as 1.81 min⁻¹. The constructed Fe⁰@NC-10/PMS system also showed prominent performance even at different solution pH values or with coexisting ions. Moreover, Fe⁰@NC-10 exhibited outstanding performance in the continuous degradation experiment. It was Fe⁰, Fe–N_x and graphitic nitrogen in Fe⁰@NC-10 that activated PMS to produce ferryl Fe-oxo species (Fe^IV=O) and ¹O₂, which collectively resulted in the removal of TC. Sixteen intermediate products were detected during TC degradation, which showed lower toxicity. This study provides a simple strategy for synthesizing an active and stable Fe⁰ nano-catalyst for TC removal by activating PMS.

{"title":"Facile synthesis of carbon nitride nanotube confined nano Fe0 for boosting activation of peroxymonosulfate towards tetracycline removal","authors":"Yajun Ji , Feiya Xu , Kun Fang , Huiyun Liu , Xiaofang Pan , Zihe Jin , Lingyun Zheng , Lele Wang","doi":"10.1016/j.eti.2025.104079","DOIUrl":"10.1016/j.eti.2025.104079","url":null,"abstract":"<div><div>The nano-sized zero-valent iron (Fe<sup>0</sup>) exhibits excellent activity for organic contaminant remediation by activating peroxymonosulfate (PMS). Its catalytic performance, however, was restricted due to its susceptibility towards oxidation and agglomeration. Thus, carbon nitride nanotube embedded nano Fe<sup>0</sup> catalysts (Fe<sup>0</sup>@NC) with various Fe contents were synthesized to simultaneously overcome the drawbacks. Fe<sup>0</sup>@NC-10 showed high specific surface area (S<sub>BET,</sub> 118.87 m<sup>2</sup>·g<sup>−1</sup>), stable crystal structure, plentiful Fe- and N-containing active sites. Under the optimal conditions (0.05 g·L<sup>−1</sup> Fe<sup>0</sup>@NC-10 and 0.15 g·L<sup>−1</sup> PMS), over 86 % tetracycline (TC) could be removed after 5 min, possessing a rate constant (<em>K</em><sub>obs</sub>) value as high as 1.81 min<sup>−1</sup>. The constructed Fe<sup>0</sup>@NC-10/PMS system also showed prominent performance even at different solution pH values or with coexisting ions. Moreover, Fe<sup>0</sup>@NC-10 exhibited outstanding performance in the continuous degradation experiment. It was Fe<sup>0</sup>, Fe–N<sub>x</sub> and graphitic nitrogen in Fe<sup>0</sup>@NC-10 that activated PMS to produce ferryl Fe-oxo species (Fe<sup>IV</sup>=O) and <sup>1</sup>O<sub>2</sub>, which collectively resulted in the removal of TC. Sixteen intermediate products were detected during TC degradation, which showed lower toxicity. This study provides a simple strategy for synthesizing an active and stable Fe<sup>0</sup> nano-catalyst for TC removal by activating PMS.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104079"},"PeriodicalIF":6.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The synergistic effect of extracellular polysaccharide-producing salt-tolerant bacteria and biochar promotes grape growth under saline-alkaline stress

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-07 DOI: 10.1016/j.eti.2025.104070

Yeqi Li , Jiqiang Zhang , Xindong Wang , Zhangzhang Feng , Enshuai Yang , Mengzhen Wu , Yuqing Jiang , Jianquan Huang , Zhen Gao , Yuanpeng Du

Grapes (Vitis vinifera) are a vital economic crop worldwide but are severely threatened by soil salinization and alkalization. While extracellular polysaccharides are known to improve soil conditions, it remains unclear how rhizosphere microorganisms that can produce extracellular polysaccharides enhance the tolerance of plants to salt-alkali conditions. This study selected Bacillus subtilis B4 and Pseudomonas resinovorans B9 based on their high levels of production of extracellular polysaccharides and subjected them to pot and field experiments. Our results demonstrated that both strains significantly promoted the growth of grape shoots, reduced the salinity of soil, and increased the levels of phosphorus and potassium in both the plants and soil. Compared to traditional B. subtilis, B9 performed better, and this was further enhanced when the strain was co-applied with biochar. 16S rRNA high-throughput sequencing was used to show that the combination of bacteria and biochar reshaped the native rhizosphere microbial community, altered its functional abundances, and improved the properties of soil, thus, ultimately promoting plant growth and enhancing salt-alkali tolerance. This study expands the microbial species available to improve the tolerance of grape to salt and ameliorate the saline-alkaline soils, thus, providing a theoretical basis for the combined application of microbial inoculants and biochar.

{"title":"The synergistic effect of extracellular polysaccharide-producing salt-tolerant bacteria and biochar promotes grape growth under saline-alkaline stress","authors":"Yeqi Li , Jiqiang Zhang , Xindong Wang , Zhangzhang Feng , Enshuai Yang , Mengzhen Wu , Yuqing Jiang , Jianquan Huang , Zhen Gao , Yuanpeng Du","doi":"10.1016/j.eti.2025.104070","DOIUrl":"10.1016/j.eti.2025.104070","url":null,"abstract":"<div><div>Grapes (<em>Vitis vinifera</em>) are a vital economic crop worldwide but are severely threatened by soil salinization and alkalization. While extracellular polysaccharides are known to improve soil conditions, it remains unclear how rhizosphere microorganisms that can produce extracellular polysaccharides enhance the tolerance of plants to salt-alkali conditions. This study selected <em>Bacillus subtilis</em> B4 and <em>Pseudomonas resinovorans</em> B9 based on their high levels of production of extracellular polysaccharides and subjected them to pot and field experiments. Our results demonstrated that both strains significantly promoted the growth of grape shoots, reduced the salinity of soil, and increased the levels of phosphorus and potassium in both the plants and soil. Compared to traditional <em>B. subtilis</em>, B9 performed better, and this was further enhanced when the strain was co-applied with biochar. 16S rRNA high-throughput sequencing was used to show that the combination of bacteria and biochar reshaped the native rhizosphere microbial community, altered its functional abundances, and improved the properties of soil, thus, ultimately promoting plant growth and enhancing salt-alkali tolerance. This study expands the microbial species available to improve the tolerance of grape to salt and ameliorate the saline-alkaline soils, thus, providing a theoretical basis for the combined application of microbial inoculants and biochar.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104070"},"PeriodicalIF":6.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acclimation strategy and nitrate supplementation significantly enhanced the ammonium tolerance of Arthrospira platensis HN5

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-07 DOI: 10.1016/j.eti.2025.104076

Qing Yu , Yali Wang , Kaixuan Wang , Wenjie Tian , Xiaobin Wen , Yi Ding , Yeguang Li , Zhongjie Wang

The highly toxic effects of ammonium on Arthrospira hinder its utilization as a nitrogen source in Arthrospira cultivation and severely limit its application in treating high-ammonium wastewater. This study revealed that both short-term (12 d) and long-term (270 d) ammonium acclimation significantly improved the ammonium tolerance of Arthrospira platensis HN5, with biomass productivities of 0.14 and 0.11 g L^–1 d^–1, respectively, and net photosynthetic oxygen evolution rates exceeding 60 and 50 μmol O₂·mg^–1·Chla·h^–1 under a lethal ammonium concentration of 15 mM. Furthermore, the ammonium tolerance of the long-term acclimated strain exhibited heritable characteristics. Additionally, nitrate (NaNO₃) concentrations of ≥ 0.1 mM were demonstrated to effectively enhance the ammonium tolerance of A. platensis HN5 in a concentration-independent manner. This study highlighted the role of acclimation and nitrate supplementation in improving the ammonium tolerance of A. platensis HN5, offering efficient and reliable strategies for ammonium utilization in the Arthrospira industry and for treating high-ammonium wastewater using Arthrospira.

{"title":"Acclimation strategy and nitrate supplementation significantly enhanced the ammonium tolerance of Arthrospira platensis HN5","authors":"Qing Yu , Yali Wang , Kaixuan Wang , Wenjie Tian , Xiaobin Wen , Yi Ding , Yeguang Li , Zhongjie Wang","doi":"10.1016/j.eti.2025.104076","DOIUrl":"10.1016/j.eti.2025.104076","url":null,"abstract":"<div><div>The highly toxic effects of ammonium on <em>Arthrospira</em> hinder its utilization as a nitrogen source in <em>Arthrospira</em> cultivation and severely limit its application in treating high-ammonium wastewater. This study revealed that both short-term (12 d) and long-term (270 d) ammonium acclimation significantly improved the ammonium tolerance of <em>Arthrospira platensis</em> HN5, with biomass productivities of 0.14 and 0.11 g L<sup>–1</sup> d<sup>–1</sup>, respectively, and net photosynthetic oxygen evolution rates exceeding 60 and 50 μmol O<sub>2</sub>·mg<sup>–1</sup>·Chla·h<sup>–1</sup> under a lethal ammonium concentration of 15 mM. Furthermore, the ammonium tolerance of the long-term acclimated strain exhibited heritable characteristics. Additionally, nitrate (NaNO<sub>3</sub>) concentrations of ≥ 0.1 mM were demonstrated to effectively enhance the ammonium tolerance of <em>A. platensis</em> HN5 in a concentration-independent manner. This study highlighted the role of acclimation and nitrate supplementation in improving the ammonium tolerance of <em>A. platensis</em> HN5, offering efficient and reliable strategies for ammonium utilization in the <em>Arthrospira</em> industry and for treating high-ammonium wastewater using <em>Arthrospira</em>.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104076"},"PeriodicalIF":6.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unseen threats: Microplastic presence in waterpipe tobacco

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-07 DOI: 10.1016/j.eti.2025.104069

Farshid Soleimani , Rasta Habibi , Hossein Arfaeinia , Mohammad Reza Masjedi , Masoumeh Tahmasbizadeh , Sara Dadipoor , Mohammad Ebrahimi kalan , Amir Zahedi

Microplastics (MPs) are a ubiquitous form of waste and an emerging public health concern. MPs have been detected in various environmental matrixes, vegetables, and foods, but no data currently exists for tobacco samples. This research aimed to evaluate the concentration and characteristics of MPs in fresh tobaccos (fruit-flavored and regular) and their post-consumption waterpipe tobacco wastes (PWTWs). Our findings showed that the mean ± SD concentration of MPs particles in fresh flavored tobacco was 4.64 ± 1.59 items/g, whereas it was 0.79 ± 0.12 items/g in regular samples (p < 0.05). In the case of PWTW of flavored tobacco, the MPs concentration was 0.82 ± 0.07 items/g, while no particle was found in the PWTW of regular samples (p < 0.05). The most dominant size of MPs in fruit-flavored tobacco was 10–50 μm, while particles < 50μm (250–500μm) were the most frequent particles in fresh regular tobacco. Fibers were the most common shape among MP particles detected in both tobacco samples, with white/transparent and black being the predominant colors across all samples. The SEM-EDS (scanning electron microscope and energy-dispersive X-ray spectroscopy) analysis revealed that carbon and oxygen (C and O) were the primary elements detected in MPs present in waterpipe tobacco, confirming that these particles are plastic in nature. To better understand the implications of these findings, further research is needed to assess additional MP characteristics, such as polymer type. Moreover, investigating the potential toxicity of these MPs is crucial for understanding their effects on smokers' health.

{"title":"Unseen threats: Microplastic presence in waterpipe tobacco","authors":"Farshid Soleimani , Rasta Habibi , Hossein Arfaeinia , Mohammad Reza Masjedi , Masoumeh Tahmasbizadeh , Sara Dadipoor , Mohammad Ebrahimi kalan , Amir Zahedi","doi":"10.1016/j.eti.2025.104069","DOIUrl":"10.1016/j.eti.2025.104069","url":null,"abstract":"<div><div>Microplastics (MPs) are a ubiquitous form of waste and an emerging public health concern. MPs have been detected in various environmental matrixes, vegetables, and foods, but no data currently exists for tobacco samples. This research aimed to evaluate the concentration and characteristics of MPs in fresh tobaccos (fruit-flavored and regular) and their post-consumption waterpipe tobacco wastes (PWTWs). Our findings showed that the mean ± SD concentration of MPs particles in fresh flavored tobacco was 4.64 ± 1.59 items/g, whereas it was 0.79 ± 0.12 items/g in regular samples (p < 0.05). In the case of PWTW of flavored tobacco, the MPs concentration was 0.82 ± 0.07 items/g, while no particle was found in the PWTW of regular samples (p < 0.05). The most dominant size of MPs in fruit-flavored tobacco was 10–50 μm, while particles < 50μm (250–500μm) were the most frequent particles in fresh regular tobacco. Fibers were the most common shape among MP particles detected in both tobacco samples, with white/transparent and black being the predominant colors across all samples. The SEM-EDS (scanning electron microscope and energy-dispersive X-ray spectroscopy) analysis revealed that carbon and oxygen (C and O) were the primary elements detected in MPs present in waterpipe tobacco, confirming that these particles are plastic in nature. To better understand the implications of these findings, further research is needed to assess additional MP characteristics, such as polymer type. Moreover, investigating the potential toxicity of these MPs is crucial for understanding their effects on smokers' health.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104069"},"PeriodicalIF":6.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between soil organic carbon fractions and microbial nutrient limitations among different woodlands in the western karst region of Hubei

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-06 DOI: 10.1016/j.eti.2025.104074

Ting Luo , Zhiteng He , Dong Xia , Yakun Xu , Lu Xia , Ting Guo , Wennian Xu , Jing Fang

In karst regions, soil organic carbon (SOC) stability and microbial activity are vital for ecosystem function, yet their response to nutrient availability remains unclear. This study investigated SOC fractions and microbial nutrient limitations in natural mixed woodland (NW), cypress woodland (CW), and stone dike artificial woodland (SW) in the Xialaoxi watershed, Hubei. Seasonal variations in labile organic carbon (LOC), microbial biomass carbon (MBC), particulate organic carbon (POC), mineral-associated organic carbon (MAOC), and enzyme activities were analyzed. The results showed that SOC levels were mainly controlled by soil microbial activity during spring and summer, whereas plant-derived organic carbon sources were the main contributors during fall and winter. NW and CW exhibited higher active organic carbon and microbial activity than SW, where SOC was predominantly mineral-bound. Deciduous broadleaved woodlands (NW) demonstrated higher carbon and nitrogen enzyme activities and larger vector angles (VA), facilitating soil carbon sequestration. Soil microbial growth was phosphorus-limited across woodlands, with microbial nutrient limitation positively correlated with SOC fractions and amino sugar content, enhancing organic carbon sequestration. Large- and medium-aggregate LAP activity and microaggregate NAG activity were key factors influencing soil aggregate SOC. PLS pathway analysis revealed woodland type affected the total nutrients, thereby altering the contents of SOC fractions, promoting the transformation activities of enzymes and ultimately impacting microbial nutrient limitation. These findings provide insights into carbon sequestration mechanisms and offer guidance for soil nutrient management and ecosystem sustainability in karst woodlands.

{"title":"Relationship between soil organic carbon fractions and microbial nutrient limitations among different woodlands in the western karst region of Hubei","authors":"Ting Luo , Zhiteng He , Dong Xia , Yakun Xu , Lu Xia , Ting Guo , Wennian Xu , Jing Fang","doi":"10.1016/j.eti.2025.104074","DOIUrl":"10.1016/j.eti.2025.104074","url":null,"abstract":"<div><div>In karst regions, soil organic carbon (SOC) stability and microbial activity are vital for ecosystem function, yet their response to nutrient availability remains unclear. This study investigated SOC fractions and microbial nutrient limitations in natural mixed woodland (NW), cypress woodland (CW), and stone dike artificial woodland (SW) in the Xialaoxi watershed, Hubei. Seasonal variations in labile organic carbon (LOC), microbial biomass carbon (MBC), particulate organic carbon (POC), mineral-associated organic carbon (MAOC), and enzyme activities were analyzed. The results showed that SOC levels were mainly controlled by soil microbial activity during spring and summer, whereas plant-derived organic carbon sources were the main contributors during fall and winter. NW and CW exhibited higher active organic carbon and microbial activity than SW, where SOC was predominantly mineral-bound. Deciduous broadleaved woodlands (NW) demonstrated higher carbon and nitrogen enzyme activities and larger vector angles (VA), facilitating soil carbon sequestration. Soil microbial growth was phosphorus-limited across woodlands, with microbial nutrient limitation positively correlated with SOC fractions and amino sugar content, enhancing organic carbon sequestration. Large- and medium-aggregate LAP activity and microaggregate NAG activity were key factors influencing soil aggregate SOC. PLS pathway analysis revealed woodland type affected the total nutrients, thereby altering the contents of SOC fractions, promoting the transformation activities of enzymes and ultimately impacting microbial nutrient limitation. These findings provide insights into carbon sequestration mechanisms and offer guidance for soil nutrient management and ecosystem sustainability in karst woodlands.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104074"},"PeriodicalIF":6.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in aerobic granular sludge from a WWTP: Adaptability of native prokaryotes and enhancement of paracetamol intermediate metabolites biodegradation

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-06 DOI: 10.1016/j.eti.2025.104073

Jorge D. Carlier , Alba Lara-Moreno , Benjamin Igbodo , Maria C. Costa

This study aimed to evaluate for the first time the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in Sequencing Batch Reactors (SBRs) with Aerobic Granular Sludge (AGS), and its effect on the biodegradation of Paracetamol, also known as Acetaminophen and N -acetyl-para-aminophenol (APAP). The bioaugmentation was effective and persisted for at least nine days after five inoculations performed in 24 days (relative abundance of M. aubagnense was 0.13 ± 0.05 % in the test reactors and 0.0079 ± 0.0008 % in the control reactors) and for eight days after seven inoculations performed in 40 days (relative abundance of M. aubagnense was 0.04 ± 0.02 % in the tests and 0.0005 ± 0.0005 % in the controls). In what concerns APAP biodegradation, the results showed a faster removal of its transformation products Hydroquinone (HQ), 2,5-dihydroxy-1,4-benzoquinone (2,5-HO-BQ) and 1,4-benzoquinone (BQ) in the bioreactors bioaugmented with the bacterial strain M. aubagnense HPB1.1 (59 % or 85 % of HQ, 67 % or 85 % of 2,5-HO-BQ and 75 % or 82 % of BQ removals, respectively for assay 1 or assay 2) in comparison to the non-bioaugmented bioreactors (15 % or 31 % of HQ, 36 % or 63 % of 2,5-HO-BQ and no removal of BQ, also for assy 1 and 2, respectively). Regarding the effect on organics and nutrients treatment, overall, the SBR conditions favored ammonia, nitrites, and organics removal. Yet, the conditions did not allow complete denitrification nor higher assimilation than release of PO₄^3-.

{"title":"Studies on the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in aerobic granular sludge from a WWTP: Adaptability of native prokaryotes and enhancement of paracetamol intermediate metabolites biodegradation","authors":"Jorge D. Carlier , Alba Lara-Moreno , Benjamin Igbodo , Maria C. Costa","doi":"10.1016/j.eti.2025.104073","DOIUrl":"10.1016/j.eti.2025.104073","url":null,"abstract":"<div><div>This study aimed to evaluate for the first time the bioaugmentation of <em>Mycolicibacterium aubagnense HPB1.</em>1 in Sequencing Batch Reactors (SBRs) with Aerobic Granular Sludge (AGS), and its effect on the biodegradation of Paracetamol, also known as Acetaminophen and N -acetyl-para-aminophenol (APAP). The bioaugmentation was effective and persisted for at least nine days after five inoculations performed in 24 days (relative abundance of <em>M. aubagnense</em> was 0.13 ± 0.05 % in the test reactors and 0.0079 ± 0.0008 % in the control reactors) and for eight days after seven inoculations performed in 40 days (relative abundance of <em>M. aubagnense</em> was 0.04 ± 0.02 % in the tests and 0.0005 ± 0.0005 % in the controls). In what concerns APAP biodegradation, the results showed a faster removal of its transformation products Hydroquinone (HQ), 2,5-dihydroxy-1,4-benzoquinone (2,5-HO-BQ) and 1,4-benzoquinone (BQ) in the bioreactors bioaugmented with the bacterial strain <em>M. aubagnense</em> HPB1.1 (59 % or 85 % of HQ, 67 % or 85 % of 2,5-HO-BQ and 75 % or 82 % of BQ removals, respectively for assay 1 or assay 2) in comparison to the non-bioaugmented bioreactors (15 % or 31 % of HQ, 36 % or 63 % of 2,5-HO-BQ and no removal of BQ, also for assy 1 and 2, respectively). Regarding the effect on organics and nutrients treatment, overall, the SBR conditions favored ammonia, nitrites, and organics removal. Yet, the conditions did not allow complete denitrification nor higher assimilation than release of PO<sub>4</sub><sup>3-</sup>.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104073"},"PeriodicalIF":6.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adding urease inhibitor into nitrogen fertilizer of hydrothermal aqueous phase decreases NH3 volatilization but may inhibit lettuce root development

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-06 DOI: 10.1016/j.eti.2025.104075

Hua Huang , Xiaoyan Liu , Xianying Shan , Jingrong Yang , Lijuan Ding , Rongyu Zhang , Jiale Dai , Yanzhong Zhen , Jian Wang , Di Guo , Yu Liu , Wenfeng Jiang , Zhirui Niu

Since hydrothermal aqueous phase (HAP) contains abundant nutrients and humic-like substances, it is developed as nitrogen (N) fertilizer to improve plant growth and soil quality. However, significant NH₃ volatilization is a major drawback in dryland soil. To solve this problem, a compound fertilizer of HAP and urea was modified with various dosage (0.25 % - 5.0 %) of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). The NH₃, N loss, and lettuce growth were evaluated in parallel soil column and pot experiments. Results showed that increasing NBPT dosage increased soil dissolved organic N (DON) and ON while decreased NH₄-N, NH₃, N loss, urease activity, and ureC. NBPT successfully inhibited ammonification. Increasing NBPT dosage increased NO₃-N but decreased PAO and AOA. The utilization rate of metabolic substrate (NH₄-N) was more important for forming NO₃-N, as evidenced by the negative correlation of NO₃-N and NH₃. The DON from HAP included N-heterocyclic compounds, which are toxic for nitrifiers. The toxic DON also significantly decreased root dry weight by 22.31 % when 5.0 % NBPT was added. The optimal additive dosages were 0.5 % and 2.0 % in the column and pot experiments, respectively, which decreased NH₃ and N loss by 45.38 % and 32.08 %. Further increasing NBPT dosage no longer decreased N loss but did harm to plant root. Therefore, this study demonstrated that an appropriate addition of NBPT to HAP fertilizer can effectively reduce ammonia volatilization and N loss. The innovative technique would promote the utilization of HAP resource within the context of sustainable dryland agriculture.

{"title":"Adding urease inhibitor into nitrogen fertilizer of hydrothermal aqueous phase decreases NH3 volatilization but may inhibit lettuce root development","authors":"Hua Huang , Xiaoyan Liu , Xianying Shan , Jingrong Yang , Lijuan Ding , Rongyu Zhang , Jiale Dai , Yanzhong Zhen , Jian Wang , Di Guo , Yu Liu , Wenfeng Jiang , Zhirui Niu","doi":"10.1016/j.eti.2025.104075","DOIUrl":"10.1016/j.eti.2025.104075","url":null,"abstract":"<div><div>Since hydrothermal aqueous phase (HAP) contains abundant nutrients and humic-like substances, it is developed as nitrogen (N) fertilizer to improve plant growth and soil quality. However, significant NH<sub>3</sub> volatilization is a major drawback in dryland soil. To solve this problem, a compound fertilizer of HAP and urea was modified with various dosage (0.25 % - 5.0 %) of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). The NH<sub>3</sub>, N loss, and lettuce growth were evaluated in parallel soil column and pot experiments. Results showed that increasing NBPT dosage increased soil dissolved organic N (DON) and ON while decreased NH<sub>4</sub>-N, NH<sub>3</sub>, N loss, urease activity, and <em>ureC</em>. NBPT successfully inhibited ammonification. Increasing NBPT dosage increased NO<sub>3</sub>-N but decreased PAO and <em>AOA</em>. The utilization rate of metabolic substrate (NH<sub>4</sub>-N) was more important for forming NO<sub>3</sub>-N, as evidenced by the negative correlation of NO<sub>3</sub>-N and NH<sub>3</sub>. The DON from HAP included N-heterocyclic compounds, which are toxic for nitrifiers. The toxic DON also significantly decreased root dry weight by 22.31 % when 5.0 % NBPT was added. The optimal additive dosages were 0.5 % and 2.0 % in the column and pot experiments, respectively, which decreased NH<sub>3</sub> and N loss by 45.38 % and 32.08 %. Further increasing NBPT dosage no longer decreased N loss but did harm to plant root. Therefore, this study demonstrated that an appropriate addition of NBPT to HAP fertilizer can effectively reduce ammonia volatilization and N loss. The innovative technique would promote the utilization of HAP resource within the context of sustainable dryland agriculture.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104075"},"PeriodicalIF":6.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the potential of distillery vinasses through upcycling: Pathways to a circular economy

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-05 DOI: 10.1016/j.eti.2025.104072

María Fernanda Ramos-Reyes , Martín Esteban González-López , Paloma Barajas-Álvarez , Christian Enrique Garcia-Garcia , Diego Antonio Tuesta-Popolizio , Solange I. Mussatto , Misael Sebastián Gradilla-Hernández

Distillery production, particularly for bioethanol and alcoholic beverages, generates substantial quantities of liquid waste known as vinasses. Typically, 10–15 liters of vinasses are produced for every liter of distilled product. This poses significant environmental challenges, including eutrophication and soil pollution. To address these issues, this systematic review comprehensively evaluates various valorization pathways for distillery vinasses management. The review analyzed 72 treatments involving vinasses from the ethanol industry, alcoholic beverage production, and their combinations with agro-industrial residues. These treatments were categorized into three major valorization pathways: waste-to-energy, waste-to-food, and waste-to-product. While the characteristics of the produced products were found to be independent of the substrate used, biotechnological treatments, such as two-stage anaerobic digestion and fungal anaerobic fermentation, demonstrated superior product yield and diversity. Fungal-based treatments stood out as the most effective, particularly for their ability to process complex substrates and enhance the production of high-value products. Furthermore, the production of polymers and enzymes using bacteria highlighted the potential for generating secondary high-value-added products from primary valorization processes, creating additional economic opportunities. Despite these promising findings, the lack of specific and comprehensive regulations addressing vinasses treatment and valorization remains a significant challenge. Moreover, tools like life cycle assessment (LCA) and techno-economic analysis, essential for ensuring sustainable and practical solutions, remain underused in this area.

{"title":"Exploring the potential of distillery vinasses through upcycling: Pathways to a circular economy","authors":"María Fernanda Ramos-Reyes , Martín Esteban González-López , Paloma Barajas-Álvarez , Christian Enrique Garcia-Garcia , Diego Antonio Tuesta-Popolizio , Solange I. Mussatto , Misael Sebastián Gradilla-Hernández","doi":"10.1016/j.eti.2025.104072","DOIUrl":"10.1016/j.eti.2025.104072","url":null,"abstract":"<div><div>Distillery production, particularly for bioethanol and alcoholic beverages, generates substantial quantities of liquid waste known as vinasses. Typically, 10–15 liters of vinasses are produced for every liter of distilled product. This poses significant environmental challenges, including eutrophication and soil pollution. To address these issues, this systematic review comprehensively evaluates various valorization pathways for distillery vinasses management. The review analyzed 72 treatments involving vinasses from the ethanol industry, alcoholic beverage production, and their combinations with agro-industrial residues. These treatments were categorized into three major valorization pathways: waste-to-energy, waste-to-food, and waste-to-product. While the characteristics of the produced products were found to be independent of the substrate used, biotechnological treatments, such as two-stage anaerobic digestion and fungal anaerobic fermentation, demonstrated superior product yield and diversity. Fungal-based treatments stood out as the most effective, particularly for their ability to process complex substrates and enhance the production of high-value products. Furthermore, the production of polymers and enzymes using bacteria highlighted the potential for generating secondary high-value-added products from primary valorization processes, creating additional economic opportunities. Despite these promising findings, the lack of specific and comprehensive regulations addressing vinasses treatment and valorization remains a significant challenge. Moreover, tools like life cycle assessment (LCA) and techno-economic analysis, essential for ensuring sustainable and practical solutions, remain underused in this area.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104072"},"PeriodicalIF":6.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of various phthalic acid esters at high concentrations by Gordonia alkanivorans GH-1 and its degradation mechanism

IF 6.7 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-02-03 DOI: 10.1016/j.eti.2025.104066

Chuanning Peng , Jie Tang , Xuan Yu , Xuerui Zhou , Mingji Wang , Yingyue Zhang , Hu Zhou , Siqi Huang , Qi Wen , Siqi Chen , Wenliang Xiang , Qing Zhang , Ting Cai

Dibutyl phthalate (DBP) is one of the most widely used phthalic acid esters (PAEs) and can easily be released into the environment and food, posing a significant threat to food safety and human health. In this study, Gordonia alkanivorans GH-1, which was previously isolated from fermented Pixian Doubanjiang in the laboratory, could effectively degrade various PAEs at high concentrations within 48 h, with the degradation efficiency of 2000 mg/L DBP reaching as high as 93.14 % under optimal degradation conditions. Furthermore, the biodegradation mechanism of DBP by strain GH-1 was explored using GC-MS and genomics technology, and the expression of key genes was validated through RT-qPCR. A novel carboxylesterase gene (est1833) was successfully cloned and expressed and the recombinant strain Escherichia coli BL21-p28a-est1833 was demonstrated the capability to degrade DBP. The interaction mechanism between the Est1833 and DBP was predicted by structural modeling and molecular docking. In summary, these findings enhance the understanding of the molecular mechanism of DBP biodegradation by G. alkanivorans GH-1 while providing a scientific foundation for future applications in environmental and food bioremediation.

{"title":"Biodegradation of various phthalic acid esters at high concentrations by Gordonia alkanivorans GH-1 and its degradation mechanism","authors":"Chuanning Peng , Jie Tang , Xuan Yu , Xuerui Zhou , Mingji Wang , Yingyue Zhang , Hu Zhou , Siqi Huang , Qi Wen , Siqi Chen , Wenliang Xiang , Qing Zhang , Ting Cai","doi":"10.1016/j.eti.2025.104066","DOIUrl":"10.1016/j.eti.2025.104066","url":null,"abstract":"<div><div>Dibutyl phthalate (DBP) is one of the most widely used phthalic acid esters (PAEs) and can easily be released into the environment and food, posing a significant threat to food safety and human health. In this study, <em>Gordonia alkanivorans</em> GH-1, which was previously isolated from fermented Pixian Doubanjiang in the laboratory, could effectively degrade various PAEs at high concentrations within 48 h, with the degradation efficiency of 2000 mg/L DBP reaching as high as 93.14 % under optimal degradation conditions. Furthermore, the biodegradation mechanism of DBP by strain GH-1 was explored using GC-MS and genomics technology, and the expression of key genes was validated through RT-qPCR. A novel carboxylesterase gene (<em>est1833</em>) was successfully cloned and expressed and the recombinant strain <em>Escherichia coli</em> BL21-p28a-<em>est1833</em> was demonstrated the capability to degrade DBP. The interaction mechanism between the Est1833 and DBP was predicted by structural modeling and molecular docking. In summary, these findings enhance the understanding of the molecular mechanism of DBP biodegradation by <em>G. alkanivorans</em> GH-1 while providing a scientific foundation for future applications in environmental and food bioremediation.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104066"},"PeriodicalIF":6.7,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143359499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0