Pub Date : 2025-02-07DOI: 10.1016/j.eti.2025.104070
Yeqi Li , Jiqiang Zhang , Xindong Wang , Zhangzhang Feng , Enshuai Yang , Mengzhen Wu , Yuqing Jiang , Jianquan Huang , Zhen Gao , Yuanpeng Du
Grapes (Vitis vinifera) are a vital economic crop worldwide but are severely threatened by soil salinization and alkalization. While extracellular polysaccharides are known to improve soil conditions, it remains unclear how rhizosphere microorganisms that can produce extracellular polysaccharides enhance the tolerance of plants to salt-alkali conditions. This study selected Bacillus subtilis B4 and Pseudomonas resinovorans B9 based on their high levels of production of extracellular polysaccharides and subjected them to pot and field experiments. Our results demonstrated that both strains significantly promoted the growth of grape shoots, reduced the salinity of soil, and increased the levels of phosphorus and potassium in both the plants and soil. Compared to traditional B. subtilis, B9 performed better, and this was further enhanced when the strain was co-applied with biochar. 16S rRNA high-throughput sequencing was used to show that the combination of bacteria and biochar reshaped the native rhizosphere microbial community, altered its functional abundances, and improved the properties of soil, thus, ultimately promoting plant growth and enhancing salt-alkali tolerance. This study expands the microbial species available to improve the tolerance of grape to salt and ameliorate the saline-alkaline soils, thus, providing a theoretical basis for the combined application of microbial inoculants and biochar.
{"title":"The synergistic effect of extracellular polysaccharide-producing salt-tolerant bacteria and biochar promotes grape growth under saline-alkaline stress","authors":"Yeqi Li , Jiqiang Zhang , Xindong Wang , Zhangzhang Feng , Enshuai Yang , Mengzhen Wu , Yuqing Jiang , Jianquan Huang , Zhen Gao , Yuanpeng Du","doi":"10.1016/j.eti.2025.104070","DOIUrl":"10.1016/j.eti.2025.104070","url":null,"abstract":"<div><div>Grapes (<em>Vitis vinifera</em>) are a vital economic crop worldwide but are severely threatened by soil salinization and alkalization. While extracellular polysaccharides are known to improve soil conditions, it remains unclear how rhizosphere microorganisms that can produce extracellular polysaccharides enhance the tolerance of plants to salt-alkali conditions. This study selected <em>Bacillus subtilis</em> B4 and <em>Pseudomonas resinovorans</em> B9 based on their high levels of production of extracellular polysaccharides and subjected them to pot and field experiments. Our results demonstrated that both strains significantly promoted the growth of grape shoots, reduced the salinity of soil, and increased the levels of phosphorus and potassium in both the plants and soil. Compared to traditional <em>B. subtilis</em>, B9 performed better, and this was further enhanced when the strain was co-applied with biochar. 16S rRNA high-throughput sequencing was used to show that the combination of bacteria and biochar reshaped the native rhizosphere microbial community, altered its functional abundances, and improved the properties of soil, thus, ultimately promoting plant growth and enhancing salt-alkali tolerance. This study expands the microbial species available to improve the tolerance of grape to salt and ameliorate the saline-alkaline soils, thus, providing a theoretical basis for the combined application of microbial inoculants and biochar.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104070"},"PeriodicalIF":6.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-07DOI: 10.1016/j.eti.2025.104069
Farshid Soleimani , Rasta Habibi , Hossein Arfaeinia , Mohammad Reza Masjedi , Masoumeh Tahmasbizadeh , Sara Dadipoor , Mohammad Ebrahimi kalan , Amir Zahedi
Microplastics (MPs) are a ubiquitous form of waste and an emerging public health concern. MPs have been detected in various environmental matrixes, vegetables, and foods, but no data currently exists for tobacco samples. This research aimed to evaluate the concentration and characteristics of MPs in fresh tobaccos (fruit-flavored and regular) and their post-consumption waterpipe tobacco wastes (PWTWs). Our findings showed that the mean ± SD concentration of MPs particles in fresh flavored tobacco was 4.64 ± 1.59 items/g, whereas it was 0.79 ± 0.12 items/g in regular samples (p < 0.05). In the case of PWTW of flavored tobacco, the MPs concentration was 0.82 ± 0.07 items/g, while no particle was found in the PWTW of regular samples (p < 0.05). The most dominant size of MPs in fruit-flavored tobacco was 10–50 μm, while particles < 50μm (250–500μm) were the most frequent particles in fresh regular tobacco. Fibers were the most common shape among MP particles detected in both tobacco samples, with white/transparent and black being the predominant colors across all samples. The SEM-EDS (scanning electron microscope and energy-dispersive X-ray spectroscopy) analysis revealed that carbon and oxygen (C and O) were the primary elements detected in MPs present in waterpipe tobacco, confirming that these particles are plastic in nature. To better understand the implications of these findings, further research is needed to assess additional MP characteristics, such as polymer type. Moreover, investigating the potential toxicity of these MPs is crucial for understanding their effects on smokers' health.
{"title":"Unseen threats: Microplastic presence in waterpipe tobacco","authors":"Farshid Soleimani , Rasta Habibi , Hossein Arfaeinia , Mohammad Reza Masjedi , Masoumeh Tahmasbizadeh , Sara Dadipoor , Mohammad Ebrahimi kalan , Amir Zahedi","doi":"10.1016/j.eti.2025.104069","DOIUrl":"10.1016/j.eti.2025.104069","url":null,"abstract":"<div><div>Microplastics (MPs) are a ubiquitous form of waste and an emerging public health concern. MPs have been detected in various environmental matrixes, vegetables, and foods, but no data currently exists for tobacco samples. This research aimed to evaluate the concentration and characteristics of MPs in fresh tobaccos (fruit-flavored and regular) and their post-consumption waterpipe tobacco wastes (PWTWs). Our findings showed that the mean ± SD concentration of MPs particles in fresh flavored tobacco was 4.64 ± 1.59 items/g, whereas it was 0.79 ± 0.12 items/g in regular samples (p < 0.05). In the case of PWTW of flavored tobacco, the MPs concentration was 0.82 ± 0.07 items/g, while no particle was found in the PWTW of regular samples (p < 0.05). The most dominant size of MPs in fruit-flavored tobacco was 10–50 μm, while particles < 50μm (250–500μm) were the most frequent particles in fresh regular tobacco. Fibers were the most common shape among MP particles detected in both tobacco samples, with white/transparent and black being the predominant colors across all samples. The SEM-EDS (scanning electron microscope and energy-dispersive X-ray spectroscopy) analysis revealed that carbon and oxygen (C and O) were the primary elements detected in MPs present in waterpipe tobacco, confirming that these particles are plastic in nature. To better understand the implications of these findings, further research is needed to assess additional MP characteristics, such as polymer type. Moreover, investigating the potential toxicity of these MPs is crucial for understanding their effects on smokers' health.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104069"},"PeriodicalIF":6.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-06DOI: 10.1016/j.eti.2025.104074
Ting Luo , Zhiteng He , Dong Xia , Yakun Xu , Lu Xia , Ting Guo , Wennian Xu , Jing Fang
In karst regions, soil organic carbon (SOC) stability and microbial activity are vital for ecosystem function, yet their response to nutrient availability remains unclear. This study investigated SOC fractions and microbial nutrient limitations in natural mixed woodland (NW), cypress woodland (CW), and stone dike artificial woodland (SW) in the Xialaoxi watershed, Hubei. Seasonal variations in labile organic carbon (LOC), microbial biomass carbon (MBC), particulate organic carbon (POC), mineral-associated organic carbon (MAOC), and enzyme activities were analyzed. The results showed that SOC levels were mainly controlled by soil microbial activity during spring and summer, whereas plant-derived organic carbon sources were the main contributors during fall and winter. NW and CW exhibited higher active organic carbon and microbial activity than SW, where SOC was predominantly mineral-bound. Deciduous broadleaved woodlands (NW) demonstrated higher carbon and nitrogen enzyme activities and larger vector angles (VA), facilitating soil carbon sequestration. Soil microbial growth was phosphorus-limited across woodlands, with microbial nutrient limitation positively correlated with SOC fractions and amino sugar content, enhancing organic carbon sequestration. Large- and medium-aggregate LAP activity and microaggregate NAG activity were key factors influencing soil aggregate SOC. PLS pathway analysis revealed woodland type affected the total nutrients, thereby altering the contents of SOC fractions, promoting the transformation activities of enzymes and ultimately impacting microbial nutrient limitation. These findings provide insights into carbon sequestration mechanisms and offer guidance for soil nutrient management and ecosystem sustainability in karst woodlands.
{"title":"Relationship between soil organic carbon fractions and microbial nutrient limitations among different woodlands in the western karst region of Hubei","authors":"Ting Luo , Zhiteng He , Dong Xia , Yakun Xu , Lu Xia , Ting Guo , Wennian Xu , Jing Fang","doi":"10.1016/j.eti.2025.104074","DOIUrl":"10.1016/j.eti.2025.104074","url":null,"abstract":"<div><div>In karst regions, soil organic carbon (SOC) stability and microbial activity are vital for ecosystem function, yet their response to nutrient availability remains unclear. This study investigated SOC fractions and microbial nutrient limitations in natural mixed woodland (NW), cypress woodland (CW), and stone dike artificial woodland (SW) in the Xialaoxi watershed, Hubei. Seasonal variations in labile organic carbon (LOC), microbial biomass carbon (MBC), particulate organic carbon (POC), mineral-associated organic carbon (MAOC), and enzyme activities were analyzed. The results showed that SOC levels were mainly controlled by soil microbial activity during spring and summer, whereas plant-derived organic carbon sources were the main contributors during fall and winter. NW and CW exhibited higher active organic carbon and microbial activity than SW, where SOC was predominantly mineral-bound. Deciduous broadleaved woodlands (NW) demonstrated higher carbon and nitrogen enzyme activities and larger vector angles (VA), facilitating soil carbon sequestration. Soil microbial growth was phosphorus-limited across woodlands, with microbial nutrient limitation positively correlated with SOC fractions and amino sugar content, enhancing organic carbon sequestration. Large- and medium-aggregate LAP activity and microaggregate NAG activity were key factors influencing soil aggregate SOC. PLS pathway analysis revealed woodland type affected the total nutrients, thereby altering the contents of SOC fractions, promoting the transformation activities of enzymes and ultimately impacting microbial nutrient limitation. These findings provide insights into carbon sequestration mechanisms and offer guidance for soil nutrient management and ecosystem sustainability in karst woodlands.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104074"},"PeriodicalIF":6.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-06DOI: 10.1016/j.eti.2025.104073
Jorge D. Carlier , Alba Lara-Moreno , Benjamin Igbodo , Maria C. Costa
This study aimed to evaluate for the first time the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in Sequencing Batch Reactors (SBRs) with Aerobic Granular Sludge (AGS), and its effect on the biodegradation of Paracetamol, also known as Acetaminophen and N -acetyl-para-aminophenol (APAP). The bioaugmentation was effective and persisted for at least nine days after five inoculations performed in 24 days (relative abundance of M. aubagnense was 0.13 ± 0.05 % in the test reactors and 0.0079 ± 0.0008 % in the control reactors) and for eight days after seven inoculations performed in 40 days (relative abundance of M. aubagnense was 0.04 ± 0.02 % in the tests and 0.0005 ± 0.0005 % in the controls). In what concerns APAP biodegradation, the results showed a faster removal of its transformation products Hydroquinone (HQ), 2,5-dihydroxy-1,4-benzoquinone (2,5-HO-BQ) and 1,4-benzoquinone (BQ) in the bioreactors bioaugmented with the bacterial strain M. aubagnense HPB1.1 (59 % or 85 % of HQ, 67 % or 85 % of 2,5-HO-BQ and 75 % or 82 % of BQ removals, respectively for assay 1 or assay 2) in comparison to the non-bioaugmented bioreactors (15 % or 31 % of HQ, 36 % or 63 % of 2,5-HO-BQ and no removal of BQ, also for assy 1 and 2, respectively). Regarding the effect on organics and nutrients treatment, overall, the SBR conditions favored ammonia, nitrites, and organics removal. Yet, the conditions did not allow complete denitrification nor higher assimilation than release of PO43-.
{"title":"Studies on the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in aerobic granular sludge from a WWTP: Adaptability of native prokaryotes and enhancement of paracetamol intermediate metabolites biodegradation","authors":"Jorge D. Carlier , Alba Lara-Moreno , Benjamin Igbodo , Maria C. Costa","doi":"10.1016/j.eti.2025.104073","DOIUrl":"10.1016/j.eti.2025.104073","url":null,"abstract":"<div><div>This study aimed to evaluate for the first time the bioaugmentation of <em>Mycolicibacterium aubagnense HPB1.</em>1 in Sequencing Batch Reactors (SBRs) with Aerobic Granular Sludge (AGS), and its effect on the biodegradation of Paracetamol, also known as Acetaminophen and N -acetyl-para-aminophenol (APAP). The bioaugmentation was effective and persisted for at least nine days after five inoculations performed in 24 days (relative abundance of <em>M. aubagnense</em> was 0.13 ± 0.05 % in the test reactors and 0.0079 ± 0.0008 % in the control reactors) and for eight days after seven inoculations performed in 40 days (relative abundance of <em>M. aubagnense</em> was 0.04 ± 0.02 % in the tests and 0.0005 ± 0.0005 % in the controls). In what concerns APAP biodegradation, the results showed a faster removal of its transformation products Hydroquinone (HQ), 2,5-dihydroxy-1,4-benzoquinone (2,5-HO-BQ) and 1,4-benzoquinone (BQ) in the bioreactors bioaugmented with the bacterial strain <em>M. aubagnense</em> HPB1.1 (59 % or 85 % of HQ, 67 % or 85 % of 2,5-HO-BQ and 75 % or 82 % of BQ removals, respectively for assay 1 or assay 2) in comparison to the non-bioaugmented bioreactors (15 % or 31 % of HQ, 36 % or 63 % of 2,5-HO-BQ and no removal of BQ, also for assy 1 and 2, respectively). Regarding the effect on organics and nutrients treatment, overall, the SBR conditions favored ammonia, nitrites, and organics removal. Yet, the conditions did not allow complete denitrification nor higher assimilation than release of PO<sub>4</sub><sup>3-</sup>.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104073"},"PeriodicalIF":6.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-06DOI: 10.1016/j.eti.2025.104075
Hua Huang , Xiaoyan Liu , Xianying Shan , Jingrong Yang , Lijuan Ding , Rongyu Zhang , Jiale Dai , Yanzhong Zhen , Jian Wang , Di Guo , Yu Liu , Wenfeng Jiang , Zhirui Niu
Since hydrothermal aqueous phase (HAP) contains abundant nutrients and humic-like substances, it is developed as nitrogen (N) fertilizer to improve plant growth and soil quality. However, significant NH3 volatilization is a major drawback in dryland soil. To solve this problem, a compound fertilizer of HAP and urea was modified with various dosage (0.25 % - 5.0 %) of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). The NH3, N loss, and lettuce growth were evaluated in parallel soil column and pot experiments. Results showed that increasing NBPT dosage increased soil dissolved organic N (DON) and ON while decreased NH4-N, NH3, N loss, urease activity, and ureC. NBPT successfully inhibited ammonification. Increasing NBPT dosage increased NO3-N but decreased PAO and AOA. The utilization rate of metabolic substrate (NH4-N) was more important for forming NO3-N, as evidenced by the negative correlation of NO3-N and NH3. The DON from HAP included N-heterocyclic compounds, which are toxic for nitrifiers. The toxic DON also significantly decreased root dry weight by 22.31 % when 5.0 % NBPT was added. The optimal additive dosages were 0.5 % and 2.0 % in the column and pot experiments, respectively, which decreased NH3 and N loss by 45.38 % and 32.08 %. Further increasing NBPT dosage no longer decreased N loss but did harm to plant root. Therefore, this study demonstrated that an appropriate addition of NBPT to HAP fertilizer can effectively reduce ammonia volatilization and N loss. The innovative technique would promote the utilization of HAP resource within the context of sustainable dryland agriculture.
{"title":"Adding urease inhibitor into nitrogen fertilizer of hydrothermal aqueous phase decreases NH3 volatilization but may inhibit lettuce root development","authors":"Hua Huang , Xiaoyan Liu , Xianying Shan , Jingrong Yang , Lijuan Ding , Rongyu Zhang , Jiale Dai , Yanzhong Zhen , Jian Wang , Di Guo , Yu Liu , Wenfeng Jiang , Zhirui Niu","doi":"10.1016/j.eti.2025.104075","DOIUrl":"10.1016/j.eti.2025.104075","url":null,"abstract":"<div><div>Since hydrothermal aqueous phase (HAP) contains abundant nutrients and humic-like substances, it is developed as nitrogen (N) fertilizer to improve plant growth and soil quality. However, significant NH<sub>3</sub> volatilization is a major drawback in dryland soil. To solve this problem, a compound fertilizer of HAP and urea was modified with various dosage (0.25 % - 5.0 %) of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). The NH<sub>3</sub>, N loss, and lettuce growth were evaluated in parallel soil column and pot experiments. Results showed that increasing NBPT dosage increased soil dissolved organic N (DON) and ON while decreased NH<sub>4</sub>-N, NH<sub>3</sub>, N loss, urease activity, and <em>ureC</em>. NBPT successfully inhibited ammonification. Increasing NBPT dosage increased NO<sub>3</sub>-N but decreased PAO and <em>AOA</em>. The utilization rate of metabolic substrate (NH<sub>4</sub>-N) was more important for forming NO<sub>3</sub>-N, as evidenced by the negative correlation of NO<sub>3</sub>-N and NH<sub>3</sub>. The DON from HAP included N-heterocyclic compounds, which are toxic for nitrifiers. The toxic DON also significantly decreased root dry weight by 22.31 % when 5.0 % NBPT was added. The optimal additive dosages were 0.5 % and 2.0 % in the column and pot experiments, respectively, which decreased NH<sub>3</sub> and N loss by 45.38 % and 32.08 %. Further increasing NBPT dosage no longer decreased N loss but did harm to plant root. Therefore, this study demonstrated that an appropriate addition of NBPT to HAP fertilizer can effectively reduce ammonia volatilization and N loss. The innovative technique would promote the utilization of HAP resource within the context of sustainable dryland agriculture.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104075"},"PeriodicalIF":6.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-05DOI: 10.1016/j.eti.2025.104072
María Fernanda Ramos-Reyes , Martín Esteban González-López , Paloma Barajas-Álvarez , Christian Enrique Garcia-Garcia , Diego Antonio Tuesta-Popolizio , Solange I. Mussatto , Misael Sebastián Gradilla-Hernández
Distillery production, particularly for bioethanol and alcoholic beverages, generates substantial quantities of liquid waste known as vinasses. Typically, 10–15 liters of vinasses are produced for every liter of distilled product. This poses significant environmental challenges, including eutrophication and soil pollution. To address these issues, this systematic review comprehensively evaluates various valorization pathways for distillery vinasses management. The review analyzed 72 treatments involving vinasses from the ethanol industry, alcoholic beverage production, and their combinations with agro-industrial residues. These treatments were categorized into three major valorization pathways: waste-to-energy, waste-to-food, and waste-to-product. While the characteristics of the produced products were found to be independent of the substrate used, biotechnological treatments, such as two-stage anaerobic digestion and fungal anaerobic fermentation, demonstrated superior product yield and diversity. Fungal-based treatments stood out as the most effective, particularly for their ability to process complex substrates and enhance the production of high-value products. Furthermore, the production of polymers and enzymes using bacteria highlighted the potential for generating secondary high-value-added products from primary valorization processes, creating additional economic opportunities. Despite these promising findings, the lack of specific and comprehensive regulations addressing vinasses treatment and valorization remains a significant challenge. Moreover, tools like life cycle assessment (LCA) and techno-economic analysis, essential for ensuring sustainable and practical solutions, remain underused in this area.
{"title":"Exploring the potential of distillery vinasses through upcycling: Pathways to a circular economy","authors":"María Fernanda Ramos-Reyes , Martín Esteban González-López , Paloma Barajas-Álvarez , Christian Enrique Garcia-Garcia , Diego Antonio Tuesta-Popolizio , Solange I. Mussatto , Misael Sebastián Gradilla-Hernández","doi":"10.1016/j.eti.2025.104072","DOIUrl":"10.1016/j.eti.2025.104072","url":null,"abstract":"<div><div>Distillery production, particularly for bioethanol and alcoholic beverages, generates substantial quantities of liquid waste known as vinasses. Typically, 10–15 liters of vinasses are produced for every liter of distilled product. This poses significant environmental challenges, including eutrophication and soil pollution. To address these issues, this systematic review comprehensively evaluates various valorization pathways for distillery vinasses management. The review analyzed 72 treatments involving vinasses from the ethanol industry, alcoholic beverage production, and their combinations with agro-industrial residues. These treatments were categorized into three major valorization pathways: waste-to-energy, waste-to-food, and waste-to-product. While the characteristics of the produced products were found to be independent of the substrate used, biotechnological treatments, such as two-stage anaerobic digestion and fungal anaerobic fermentation, demonstrated superior product yield and diversity. Fungal-based treatments stood out as the most effective, particularly for their ability to process complex substrates and enhance the production of high-value products. Furthermore, the production of polymers and enzymes using bacteria highlighted the potential for generating secondary high-value-added products from primary valorization processes, creating additional economic opportunities. Despite these promising findings, the lack of specific and comprehensive regulations addressing vinasses treatment and valorization remains a significant challenge. Moreover, tools like life cycle assessment (LCA) and techno-economic analysis, essential for ensuring sustainable and practical solutions, remain underused in this area.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104072"},"PeriodicalIF":6.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-03DOI: 10.1016/j.eti.2025.104066
Chuanning Peng , Jie Tang , Xuan Yu , Xuerui Zhou , Mingji Wang , Yingyue Zhang , Hu Zhou , Siqi Huang , Qi Wen , Siqi Chen , Wenliang Xiang , Qing Zhang , Ting Cai
Dibutyl phthalate (DBP) is one of the most widely used phthalic acid esters (PAEs) and can easily be released into the environment and food, posing a significant threat to food safety and human health. In this study, Gordonia alkanivorans GH-1, which was previously isolated from fermented Pixian Doubanjiang in the laboratory, could effectively degrade various PAEs at high concentrations within 48 h, with the degradation efficiency of 2000 mg/L DBP reaching as high as 93.14 % under optimal degradation conditions. Furthermore, the biodegradation mechanism of DBP by strain GH-1 was explored using GC-MS and genomics technology, and the expression of key genes was validated through RT-qPCR. A novel carboxylesterase gene (est1833) was successfully cloned and expressed and the recombinant strain Escherichia coli BL21-p28a-est1833 was demonstrated the capability to degrade DBP. The interaction mechanism between the Est1833 and DBP was predicted by structural modeling and molecular docking. In summary, these findings enhance the understanding of the molecular mechanism of DBP biodegradation by G. alkanivorans GH-1 while providing a scientific foundation for future applications in environmental and food bioremediation.
{"title":"Biodegradation of various phthalic acid esters at high concentrations by Gordonia alkanivorans GH-1 and its degradation mechanism","authors":"Chuanning Peng , Jie Tang , Xuan Yu , Xuerui Zhou , Mingji Wang , Yingyue Zhang , Hu Zhou , Siqi Huang , Qi Wen , Siqi Chen , Wenliang Xiang , Qing Zhang , Ting Cai","doi":"10.1016/j.eti.2025.104066","DOIUrl":"10.1016/j.eti.2025.104066","url":null,"abstract":"<div><div>Dibutyl phthalate (DBP) is one of the most widely used phthalic acid esters (PAEs) and can easily be released into the environment and food, posing a significant threat to food safety and human health. In this study, <em>Gordonia alkanivorans</em> GH-1, which was previously isolated from fermented Pixian Doubanjiang in the laboratory, could effectively degrade various PAEs at high concentrations within 48 h, with the degradation efficiency of 2000 mg/L DBP reaching as high as 93.14 % under optimal degradation conditions. Furthermore, the biodegradation mechanism of DBP by strain GH-1 was explored using GC-MS and genomics technology, and the expression of key genes was validated through RT-qPCR. A novel carboxylesterase gene (<em>est1833</em>) was successfully cloned and expressed and the recombinant strain <em>Escherichia coli</em> BL21-p28a-<em>est1833</em> was demonstrated the capability to degrade DBP. The interaction mechanism between the Est1833 and DBP was predicted by structural modeling and molecular docking. In summary, these findings enhance the understanding of the molecular mechanism of DBP biodegradation by <em>G. alkanivorans</em> GH-1 while providing a scientific foundation for future applications in environmental and food bioremediation.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104066"},"PeriodicalIF":6.7,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143359499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.eti.2024.103925
Zhaoxia Li , Sainan Luo , Xuan Li , Tianming Chen , Weixing Ma , Liqiang Cui , Songnian Hu , Cheng Ding , Jianing Geng
The impact of wastewater on the ecological functioning of constructed wetland systems can be assessed by analyzing the microbial diversity, profiling, and community structure of soils irrigated with wastewater. In this study, we utilized in situ, culture-independent 16S rRNA sequencing technology to comprehensively investigate the biodiversity, abundance, and structure of bacterial communities in reed field soils irrigated with pretreated pulp and paper wastewater (PPW) and freshwater. Our results revealed significant differences in bacterial communities across freshwater-irrigated wetlands, short-term wastewater-irrigated wetlands, and long-term wastewater-irrigated wetlands. Redundancy analysis and Spearman's correlation analysis demonstrated that soil pollutants (TX and AOX), nutrients (TN and TP), and heavy metals significantly influenced the microbial community structure. These findings underscore the importance of microbial monitoring and suggest that irrigation should be discontinued after 10 years to facilitate the recovery of wetland ecosystems.
{"title":"Influence of irrigation with pulp and paper mill wastewater on bacterial communities of reed fields","authors":"Zhaoxia Li , Sainan Luo , Xuan Li , Tianming Chen , Weixing Ma , Liqiang Cui , Songnian Hu , Cheng Ding , Jianing Geng","doi":"10.1016/j.eti.2024.103925","DOIUrl":"10.1016/j.eti.2024.103925","url":null,"abstract":"<div><div>The impact of wastewater on the ecological functioning of constructed wetland systems can be assessed by analyzing the microbial diversity, profiling, and community structure of soils irrigated with wastewater. In this study, we utilized in situ, culture-independent 16S rRNA sequencing technology to comprehensively investigate the biodiversity, abundance, and structure of bacterial communities in reed field soils irrigated with pretreated pulp and paper wastewater (PPW) and freshwater. Our results revealed significant differences in bacterial communities across freshwater-irrigated wetlands, short-term wastewater-irrigated wetlands, and long-term wastewater-irrigated wetlands. Redundancy analysis and Spearman's correlation analysis demonstrated that soil pollutants (TX and AOX), nutrients (TN and TP), and heavy metals significantly influenced the microbial community structure. These findings underscore the importance of microbial monitoring and suggest that irrigation should be discontinued after 10 years to facilitate the recovery of wetland ecosystems.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"37 ","pages":"Article 103925"},"PeriodicalIF":6.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arsenic present in groundwater is a global problem that requires innovative remediation, this study used the Spherical Agglomeration Technique (SAT) using Fe(OH)₃ as adsorbent, Agave extract as biosurfactant, and olive oil as humectant. The primary objective was to evaluate the olive oil versus n-heptane efficacy in arsenic removal within the SAT methodology while assessing the synergistic effects of varying adsorbent (AD) and humectant (HD) dosages. Experiments evaluated arsenic-contaminated water models and groundwater with adsorbent concentrations ranging from 30 to 150 g Fe(OH)₃/L and humectant dosages from 3.0 to 9.0 mL Hum/g TMC. Initial arsenic levels in aqueous models were established at 100 µg/L, maintaining pH at 7.0, 300 rpm stirring speed, and 0.5 g Ext/g TMC surfactant dosage. The SAT process efficiency was evaluated under optimized conditions for aqueous models and groundwater samples. In aqueous models, optimal arsenic removal was achieved with a AD of 150 g Fe(OH)₃/L and HD of 6 mL Hum/gTMC, resulting in final arsenic concentrations of 3.2 µg/L, with iron and calcium levels compliant with regulatory limits. For groundwater, reduced dosage of 30 g Fe(OH)₃/L and higher humectant dosage of 9.0 mL Hum/g TMC enabled arsenic reduction to 3.5 µg/L while maintaining minimal final concentrations of iron (0.01 mg/L) and calcium; the results highlight olive oil's superior performance over n-heptane, demonstrating the importance of precise dosage optimization for maximum efficacy, this study positions olive oil as a viable and sustainable solution for treating arsenic in water via SAT, which can be tailored to specific circumstances.
{"title":"Arsenic removal by Spherical Agglomeration Technique in groundwater using vegetable oil instead of n-heptane","authors":"O.A. Torres-Corral , J.C. Rojas-Montes , S. Valle-Cervantes , F.A. Alcazar-Medina","doi":"10.1016/j.eti.2024.103955","DOIUrl":"10.1016/j.eti.2024.103955","url":null,"abstract":"<div><div>Arsenic present in groundwater is a global problem that requires innovative remediation, this study used the Spherical Agglomeration Technique (SAT) using Fe(OH)₃ as adsorbent, Agave extract as biosurfactant, and olive oil as humectant. The primary objective was to evaluate the olive oil versus n-heptane efficacy in arsenic removal within the SAT methodology while assessing the synergistic effects of varying adsorbent (AD) and humectant (HD) dosages. Experiments evaluated arsenic-contaminated water models and groundwater with adsorbent concentrations ranging from 30 to 150 g <sub>Fe(OH)₃</sub>/L and humectant dosages from 3.0 to 9.0 mL <sub>Hum</sub>/g <sub>TMC</sub>. Initial arsenic levels in aqueous models were established at 100 µg/L, maintaining pH at 7.0, 300 rpm stirring speed, and 0.5 g <sub>Ext</sub>/g <sub>TMC</sub> surfactant dosage. The SAT process efficiency was evaluated under optimized conditions for aqueous models and groundwater samples. In aqueous models, optimal arsenic removal was achieved with a AD of 150 g <sub>Fe(OH)₃</sub>/L and HD of 6 mL <sub>Hum</sub>/g<sub>TMC</sub>, resulting in final arsenic concentrations of 3.2 µg/L, with iron and calcium levels compliant with regulatory limits. For groundwater, reduced dosage of 30 g <sub>Fe(OH)₃</sub>/L and higher humectant dosage of 9.0 mL <sub>Hum</sub>/g <sub>TMC</sub> enabled arsenic reduction to 3.5 µg/L while maintaining minimal final concentrations of iron (0.01 mg/L) and calcium; the results highlight olive oil's superior performance over n-heptane, demonstrating the importance of precise dosage optimization for maximum efficacy, this study positions olive oil as a viable and sustainable solution for treating arsenic in water via SAT, which can be tailored to specific circumstances.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"37 ","pages":"Article 103955"},"PeriodicalIF":6.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143178496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.eti.2024.103919
Anqi Wang, Jingjing Xue, Bo Pang, Haozhe Wang, Zhicheng Xu, Ruixue Chang, Yanming Li
The fermented mixtures at different composting stages exhibit varying physicochemical and microbiological properties, adding them to compost, which may show different biological enhancement abilities. This study analyzed the efficiency of fermented mixtures in mitigating pollutant gas emissions during composting, through the co-composting of food waste and sawdust, supplemented with 20 % fermented mixtures from different composting stages: thermophilic (TS), cooling (CS), and mature (MS). Results revealed that fermented mixtures affected the composting process and pollutant gas emissions by regulating bacterial communities. The TS treatment increased the abundance of high-temperature resistant organic matter degrading bacteria, thereby effectively promoting temperature and reaching the highest peak temperature (> 70 °C) in just 2 days. Among all the treatments, TS exhibited the most pronounced reduction in N2O emissions, inhibiting the proliferation of bacteria engaged in nitrite respiration, which resulted in reducing overall N2O emissions by 20.7 %. Furthermore, TS outperformed both CK and MS treatments regarding CO2 reduction, achieving declines of 11.2 % and 20.2 %, respectively. The MS treatment diminished the abundance of acidogens and methanogens bacteria, such as Lactobacillus, Weissella, and Leuconostoc, resulting in a 24.2 % reduction in methane emissions compared to CK. In contrast, the CS treatment had minimal effect, reducing methane emissions by 4.2 %. In conclusion, thermophilic compost demonstrated superior effectiveness in reducing pollutant gas emissions compared to other fermented mixtures. Additionally, owing to its economic advantages, thermophilic compost can be produced significantly faster than the others. Therefore, thermophilic compost is recommended as a bioaugmentation technology that synergistically offers environmental benefits and cost-effectiveness.
{"title":"Bioaugmentation strategy for mitigating pollutant gas emissions in food waste composting using fermented mixtures","authors":"Anqi Wang, Jingjing Xue, Bo Pang, Haozhe Wang, Zhicheng Xu, Ruixue Chang, Yanming Li","doi":"10.1016/j.eti.2024.103919","DOIUrl":"10.1016/j.eti.2024.103919","url":null,"abstract":"<div><div>The fermented mixtures at different composting stages exhibit varying physicochemical and microbiological properties, adding them to compost, which may show different biological enhancement abilities. This study analyzed the efficiency of fermented mixtures in mitigating pollutant gas emissions during composting, through the co-composting of food waste and sawdust, supplemented with 20 % fermented mixtures from different composting stages: thermophilic (TS), cooling (CS), and mature (MS). Results revealed that fermented mixtures affected the composting process and pollutant gas emissions by regulating bacterial communities. The TS treatment increased the abundance of high-temperature resistant organic matter degrading bacteria, thereby effectively promoting temperature and reaching the highest peak temperature (> 70 °C) in just 2 days. Among all the treatments, TS exhibited the most pronounced reduction in N<sub>2</sub>O emissions, inhibiting the proliferation of bacteria engaged in nitrite respiration, which resulted in reducing overall N<sub>2</sub>O emissions by 20.7 %. Furthermore, TS outperformed both CK and MS treatments regarding CO<sub>2</sub> reduction, achieving declines of 11.2 % and 20.2 %, respectively. The MS treatment diminished the abundance of acidogens and methanogens bacteria, such as <em>Lactobacillus</em>, <em>Weissella</em>, and <em>Leuconostoc</em>, resulting in a 24.2 % reduction in methane emissions compared to CK. In contrast, the CS treatment had minimal effect, reducing methane emissions by 4.2 %. In conclusion, thermophilic compost demonstrated superior effectiveness in reducing pollutant gas emissions compared to other fermented mixtures. Additionally, owing to its economic advantages, thermophilic compost can be produced significantly faster than the others. Therefore, thermophilic compost is recommended as a bioaugmentation technology that synergistically offers environmental benefits and cost-effectiveness.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"37 ","pages":"Article 103919"},"PeriodicalIF":6.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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