首页 > 最新文献

Environmental Epigenetics最新文献

英文 中文
Potential (mis)use of epigenetic age estimators by private companies and public agencies: human rights law should provide ethical guidance 私营公司和公共机构可能(错误地)使用表观遗传年龄估计器:人权法应提供道德指导
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-07-01 DOI: 10.1093/EEP/DVZ018
C. Dupras, S. Beck, M. Rothstein, A. Berner, Katie M. Saulnier, Miriam Pinkesz, A. Prince, Stamatina Liosi, Lingqiao Song, Y. Joly
{"title":"Potential (mis)use of epigenetic age estimators by private companies and public agencies: human rights law should provide ethical guidance","authors":"C. Dupras, S. Beck, M. Rothstein, A. Berner, Katie M. Saulnier, Miriam Pinkesz, A. Prince, Stamatina Liosi, Lingqiao Song, Y. Joly","doi":"10.1093/EEP/DVZ018","DOIUrl":"https://doi.org/10.1093/EEP/DVZ018","url":null,"abstract":"","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41579254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Three-dimensional testicular organoids as novel in vitro models of testicular biology and toxicology 三维睾丸类器官作为睾丸生物学和毒理学的新型体外模型
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-07-01 DOI: 10.1093/eep/dvz011
S. Sakib, A. Voigt, T. Goldsmith, I. Dobrinski
Abstract Organoids are three dimensional structures consisting of multiple cell types that recapitulate the cellular architecture and functionality of native organs. Over the last decade, the advent of organoid research has opened up many avenues for basic and translational studies. Following suit of other disciplines, research groups working in the field of male reproductive biology have started establishing and characterizing testicular organoids. The three-dimensional architectural and functional similarities of organoids to their tissue of origin facilitate study of complex cell interactions, tissue development and establishment of representative, scalable models for drug and toxicity screening. In this review, we discuss the current state of testicular organoid research, their advantages over conventional monolayer culture and their potential applications in the field of reproductive biology and toxicology.
摘要类器官是由多种细胞类型组成的三维结构,概括了天然器官的细胞结构和功能。在过去的十年里,类器官研究的出现为基础研究和转化研究开辟了许多途径。继其他学科之后,男性生殖生物学领域的研究小组已经开始建立和表征睾丸类器官。类器官与其来源组织的三维结构和功能相似性有助于研究复杂的细胞相互作用、组织发育,并建立具有代表性的、可扩展的药物和毒性筛选模型。在这篇综述中,我们讨论了睾丸类器官的研究现状,它们与传统单层培养相比的优势,以及它们在生殖生物学和毒理学领域的潜在应用。
{"title":"Three-dimensional testicular organoids as novel in vitro models of testicular biology and toxicology","authors":"S. Sakib, A. Voigt, T. Goldsmith, I. Dobrinski","doi":"10.1093/eep/dvz011","DOIUrl":"https://doi.org/10.1093/eep/dvz011","url":null,"abstract":"Abstract Organoids are three dimensional structures consisting of multiple cell types that recapitulate the cellular architecture and functionality of native organs. Over the last decade, the advent of organoid research has opened up many avenues for basic and translational studies. Following suit of other disciplines, research groups working in the field of male reproductive biology have started establishing and characterizing testicular organoids. The three-dimensional architectural and functional similarities of organoids to their tissue of origin facilitate study of complex cell interactions, tissue development and establishment of representative, scalable models for drug and toxicity screening. In this review, we discuss the current state of testicular organoid research, their advantages over conventional monolayer culture and their potential applications in the field of reproductive biology and toxicology.","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvz011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47656991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 25
Early postnatal overnutrition accelerates aging-associated epigenetic drift in pancreatic islets 出生后早期营养过剩加速胰岛衰老相关的表观遗传漂移
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-07-01 DOI: 10.1093/eep/dvz015
Ge Li, T. D. Petkova, Eleonora Laritsky, Noah J. Kessler, Maria S. Baker, Shaoyu Zhu, R. Waterland
Abstract Pancreatic islets of type 2 diabetes patients have altered DNA methylation, contributing to islet dysfunction and the onset of type 2 diabetes. The cause of these epigenetic alterations is largely unknown. We set out to test whether (i) islet DNA methylation would change with aging and (ii) early postnatal overnutrition would persistently alter DNA methylation. We performed genome-scale DNA methylation profiling in islets from postnatally over-nourished (suckled in a small litter) and control male mice at both postnatal day 21 and postnatal day 180. DNA methylation differences were validated using quantitative bisulfite pyrosequencing, and associations with expression were assessed by RT-PCR. We discovered that genomic regions that are hypermethylated in exocrine relative to endocrine pancreas tend to gain methylation in islets during aging (R2 = 0.33, P < 0.0001). These methylation differences were inversely correlated with mRNA expression of genes relevant to β cell function [including Rab3b (Ras-related protein Rab-3B), Cacnb3 (voltage-dependent L-type calcium channel subunit 3), Atp2a3 (sarcoplasmic/endoplasmic reticulum calcium ATPase 3) and Ins2 (insulin 2)]. Relative to control, small litter islets showed DNA methylation differences directly after weaning and in adulthood, but few of these were present at both ages. Surprisingly, we found substantial overlap of methylated loci caused by aging and small litter feeding, suggesting that the age-associated gain of DNA methylation happened much earlier in small litter islets than control islets. Our results provide the novel insights that aging-associated DNA methylation increases reflect an epigenetic drift toward the exocrine pancreas epigenome, and that early postnatal overnutrition may accelerate this process.
摘要2型糖尿病患者的胰岛DNA甲基化发生改变,导致胰岛功能障碍和2型糖尿病的发作。这些表观遗传学改变的原因在很大程度上是未知的。我们开始测试(i)胰岛DNA甲基化是否会随着年龄的增长而变化,以及(ii)出生后早期营养过剩是否会持续改变DNA甲基化。我们在出生后第21天和出生后第180天对出生后营养过剩(在小窝中哺乳)和对照雄性小鼠的胰岛进行了基因组规模的DNA甲基化分析。DNA甲基化差异通过亚硫酸氢盐焦磷酸氢盐定量测序进行验证,并通过RT-PCR评估与表达的相关性。我们发现,相对于内分泌胰腺,外分泌中甲基化程度较高的基因组区域在衰老过程中往往会在胰岛中获得甲基化(R2=0.33,P < 0.0001)。这些甲基化差异与β细胞功能相关基因的mRNA表达呈负相关[包括Rab3b(Ras相关蛋白Rab-3B)、Cacnb3(电压依赖性L型钙通道亚基3)、Atp2a3(肌浆/内质网钙ATP酶3)和Ins2(胰岛素2)]。与对照组相比,断奶后和成年后,小窝胰岛直接表现出DNA甲基化差异,但在两个年龄段都很少出现这种差异。令人惊讶的是,我们发现衰老和小产仔喂养引起的甲基化基因座有很大的重叠,这表明与年龄相关的DNA甲基化增加在小产仔胰岛中发生得比对照胰岛早得多。我们的研究结果提供了新的见解,即衰老相关的DNA甲基化增加反映了表观遗传学向外分泌胰腺表观基因组的漂移,出生后早期营养过剩可能会加速这一过程。
{"title":"Early postnatal overnutrition accelerates aging-associated epigenetic drift in pancreatic islets","authors":"Ge Li, T. D. Petkova, Eleonora Laritsky, Noah J. Kessler, Maria S. Baker, Shaoyu Zhu, R. Waterland","doi":"10.1093/eep/dvz015","DOIUrl":"https://doi.org/10.1093/eep/dvz015","url":null,"abstract":"Abstract Pancreatic islets of type 2 diabetes patients have altered DNA methylation, contributing to islet dysfunction and the onset of type 2 diabetes. The cause of these epigenetic alterations is largely unknown. We set out to test whether (i) islet DNA methylation would change with aging and (ii) early postnatal overnutrition would persistently alter DNA methylation. We performed genome-scale DNA methylation profiling in islets from postnatally over-nourished (suckled in a small litter) and control male mice at both postnatal day 21 and postnatal day 180. DNA methylation differences were validated using quantitative bisulfite pyrosequencing, and associations with expression were assessed by RT-PCR. We discovered that genomic regions that are hypermethylated in exocrine relative to endocrine pancreas tend to gain methylation in islets during aging (R2 = 0.33, P < 0.0001). These methylation differences were inversely correlated with mRNA expression of genes relevant to β cell function [including Rab3b (Ras-related protein Rab-3B), Cacnb3 (voltage-dependent L-type calcium channel subunit 3), Atp2a3 (sarcoplasmic/endoplasmic reticulum calcium ATPase 3) and Ins2 (insulin 2)]. Relative to control, small litter islets showed DNA methylation differences directly after weaning and in adulthood, but few of these were present at both ages. Surprisingly, we found substantial overlap of methylated loci caused by aging and small litter feeding, suggesting that the age-associated gain of DNA methylation happened much earlier in small litter islets than control islets. Our results provide the novel insights that aging-associated DNA methylation increases reflect an epigenetic drift toward the exocrine pancreas epigenome, and that early postnatal overnutrition may accelerate this process.","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvz015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46378383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Tissue-specific changes in Srebf1 and Srebf2 expression and DNA methylation with perinatal phthalate exposure. Srebf1和Srebf2表达和DNA甲基化与围产期邻苯二甲酸盐暴露的组织特异性变化
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-06-20 eCollection Date: 2019-04-01 DOI: 10.1093/eep/dvz009
Laura Moody, Diego Hernández-Saavedra, Daniel G Kougias, Hong Chen, Janice M Juraska, Yuan-Xiang Pan

Perinatal exposure to endocrine disrupting chemicals negatively impacts health, but the mechanism by which such toxicants damage long-term reproductive and metabolic function is unknown. Lipid metabolism plays a pivotal role in steroid hormone synthesis as well as energy utilization and storage; thus, aberrant lipid regulation may contribute to phthalate-driven health impairments. In order to test this hypothesis, we specifically examined epigenetic disruptions in lipid metabolism pathways after perinatal phthalate exposure. During gestation and lactation, pregnant Long-Evans rat dams were fed environmentally relevant doses of phthalate mixture: 0 (CON), 200 (LO), or 1000 (HI) µg/kg body weight/day. On PND90, male offspring in the LO and HI groups had higher body weights than CON rats. Gene expression of lipid metabolism pathways was altered in testis and adipose tissue of males exposed to the HI phthalate dosage. Specifically, Srebf1 was downregulated in testis and Srebf2 was upregulated in adipose tissue. In testis of HI rats, DNA methylation was increased at two loci and reduced at one other site surrounding Srebf1 transcription start site. In adipose tissue of HI rats, we observed increased DNA methylation at one region within the first intron of Srebf2. Computational analysis revealed several potential transcriptional regulator binding sites, suggesting functional relevance of the identified differentially methylated CpGs. Overall, we show that perinatal phthalate exposure affects lipid metabolism gene expression in a tissue-specific manner possibly through altering DNA methylation of Srebf1 and Srebf2.

围产期接触内分泌干扰化学物质会对健康产生负面影响,但这些有毒物质损害长期生殖和代谢功能的机制尚不清楚。脂质代谢在类固醇激素合成、能量利用和储存中起关键作用;因此,异常的脂质调节可能导致邻苯二甲酸盐驱动的健康损害。为了验证这一假设,我们特别检查了围产期邻苯二甲酸盐暴露后脂质代谢途径的表观遗传破坏。在妊娠和哺乳期,给怀孕的Long-Evans大鼠喂食环境相关剂量的邻苯二甲酸盐混合物:0 (CON)、200 (LO)或1000 (HI)µg/kg体重/天。在PND90上,LO组和HI组雄性后代的体重高于对照组。暴露于高邻苯二甲酸盐剂量的男性睾丸和脂肪组织中脂质代谢途径的基因表达发生改变。具体来说,Srebf1在睾丸中下调,而Srebf2在脂肪组织中上调。在HI大鼠的睾丸中,DNA甲基化在两个位点增加,在Srebf1转录起始位点周围的另一个位点减少。在HI大鼠的脂肪组织中,我们观察到Srebf2第一个内含子内一个区域的DNA甲基化增加。计算分析揭示了几个潜在的转录调节因子结合位点,表明鉴定的差异甲基化CpGs具有功能相关性。总的来说,我们表明围产期邻苯二甲酸盐暴露以组织特异性的方式影响脂质代谢基因表达,可能通过改变Srebf1和Srebf2的DNA甲基化。
{"title":"Tissue-specific changes in <i>Srebf1</i> and <i>Srebf2</i> expression and DNA methylation with perinatal phthalate exposure.","authors":"Laura Moody,&nbsp;Diego Hernández-Saavedra,&nbsp;Daniel G Kougias,&nbsp;Hong Chen,&nbsp;Janice M Juraska,&nbsp;Yuan-Xiang Pan","doi":"10.1093/eep/dvz009","DOIUrl":"https://doi.org/10.1093/eep/dvz009","url":null,"abstract":"<p><p>Perinatal exposure to endocrine disrupting chemicals negatively impacts health, but the mechanism by which such toxicants damage long-term reproductive and metabolic function is unknown. Lipid metabolism plays a pivotal role in steroid hormone synthesis as well as energy utilization and storage; thus, aberrant lipid regulation may contribute to phthalate-driven health impairments. In order to test this hypothesis, we specifically examined epigenetic disruptions in lipid metabolism pathways after perinatal phthalate exposure. During gestation and lactation, pregnant Long-Evans rat dams were fed environmentally relevant doses of phthalate mixture: 0 (CON), 200 (LO), or 1000 (HI) µg/kg body weight/day. On PND90, male offspring in the LO and HI groups had higher body weights than CON rats. Gene expression of lipid metabolism pathways was altered in testis and adipose tissue of males exposed to the HI phthalate dosage. Specifically, <i>Srebf1</i> was downregulated in testis and <i>Srebf2</i> was upregulated in adipose tissue. In testis of HI rats, DNA methylation was increased at two loci and reduced at one other site surrounding <i>Srebf1</i> transcription start site. In adipose tissue of HI rats, we observed increased DNA methylation at one region within the first intron of <i>Srebf2</i>. Computational analysis revealed several potential transcriptional regulator binding sites, suggesting functional relevance of the identified differentially methylated CpGs. Overall, we show that perinatal phthalate exposure affects lipid metabolism gene expression in a tissue-specific manner possibly through altering DNA methylation of <i>Srebf1</i> and <i>Srebf2</i>.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"5 2","pages":"dvz009"},"PeriodicalIF":3.8,"publicationDate":"2019-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvz009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37365416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Sperm epimutation biomarkers of obesity and pathologies following DDT induced epigenetic transgenerational inheritance of disease. 滴滴涕诱导的表观遗传跨代遗传疾病后的肥胖和病理的精子上皮化生物标志物。
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-05-27 eCollection Date: 2019-04-01 DOI: 10.1093/eep/dvz008
Stephanie E King, Margaux McBirney, Daniel Beck, Ingrid Sadler-Riggleman, Eric Nilsson, Michael K Skinner

Dichlorodiphenyltrichloroethane (DDT) has previously been shown to promote the epigenetic transgenerational inheritance of adult onset disease in rats. The current study investigated the potential that sperm epimutation biomarkers can be used to identify ancestral induced transgenerational obesity and associated pathologies. Gestating F0 generational female rats were transiently exposed to DDT during fetal gonadal sex determination, and the incidence of adult-onset pathologies was assessed in the subsequent F1, F2, and F3 generations. In addition, sperm differential DNA methylation regions (DMRs) that were associated with specific pathologies in the transgenerational F3 generation males were investigated. There was an increase of testis disease and early-onset puberty in the F2 generation DDT lineage males. The F3 generation males and females had significant increases in the incidence of obesity and multiple disease. The F3 generation DDT males also had significant increases in testis disease, prostate disease, and late onset puberty. The F3 generation DDT females had increases in ovarian and kidney disease. Epigenetic alterations of the germline are required for the transgenerational inheritance of pathology. Therefore, the F3 generation sperm was collected to examine DMRs for the ancestrally exposed DDT male population. Unique sets of DMRs were associated with late onset puberty, prostate disease, kidney disease, testis disease, obesity, and multiple disease pathologies. Gene associations with the DMR were also identified. The epigenetic DMR signatures identified for these pathologies provide potential biomarkers for transgenerationally inherited disease susceptibility.

二氯二苯三氯乙烷(DDT)先前已被证明可促进大鼠成年发病疾病的表观遗传跨代遗传。目前的研究调查了精子上皮化生物标志物可用于识别祖先诱导的跨代肥胖和相关病理的潜力。在胎儿性腺性别测定期间,将妊娠第0代雌性大鼠短暂暴露于DDT,并在随后的F1、F2和F3代中评估成年发病的发病率。此外,我们还研究了与跨代F3代男性特定病理相关的精子差异DNA甲基化区(DMRs)。滴滴涕系F2代男性睾丸疾病和早发性青春期增加。F3代男性和女性肥胖和多种疾病的发病率显著增加。F3代DDT雄性的睾丸疾病、前列腺疾病和青春期延迟也显著增加。F3代滴滴涕雌性卵巢和肾脏疾病增加。生殖系的表观遗传改变是病理跨代遗传所必需的。因此,我们收集了F3代精子来检测祖先暴露滴滴涕的男性群体的dmr。独特的DMRs组与晚发性青春期、前列腺疾病、肾脏疾病、睾丸疾病、肥胖和多种疾病病理相关。与DMR的基因关联也被确定。这些病理的表观遗传DMR特征为跨代遗传疾病易感性提供了潜在的生物标志物。
{"title":"Sperm epimutation biomarkers of obesity and pathologies following DDT induced epigenetic transgenerational inheritance of disease.","authors":"Stephanie E King,&nbsp;Margaux McBirney,&nbsp;Daniel Beck,&nbsp;Ingrid Sadler-Riggleman,&nbsp;Eric Nilsson,&nbsp;Michael K Skinner","doi":"10.1093/eep/dvz008","DOIUrl":"https://doi.org/10.1093/eep/dvz008","url":null,"abstract":"<p><p>Dichlorodiphenyltrichloroethane (DDT) has previously been shown to promote the epigenetic transgenerational inheritance of adult onset disease in rats. The current study investigated the potential that sperm epimutation biomarkers can be used to identify ancestral induced transgenerational obesity and associated pathologies. Gestating F0 generational female rats were transiently exposed to DDT during fetal gonadal sex determination, and the incidence of adult-onset pathologies was assessed in the subsequent F1, F2, and F3 generations. In addition, sperm differential DNA methylation regions (DMRs) that were associated with specific pathologies in the transgenerational F3 generation males were investigated. There was an increase of testis disease and early-onset puberty in the F2 generation DDT lineage males. The F3 generation males and females had significant increases in the incidence of obesity and multiple disease. The F3 generation DDT males also had significant increases in testis disease, prostate disease, and late onset puberty. The F3 generation DDT females had increases in ovarian and kidney disease. Epigenetic alterations of the germline are required for the transgenerational inheritance of pathology. Therefore, the F3 generation sperm was collected to examine DMRs for the ancestrally exposed DDT male population. Unique sets of DMRs were associated with late onset puberty, prostate disease, kidney disease, testis disease, obesity, and multiple disease pathologies. Gene associations with the DMR were also identified. The epigenetic DMR signatures identified for these pathologies provide potential biomarkers for transgenerationally inherited disease susceptibility.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"5 2","pages":"dvz008"},"PeriodicalIF":3.8,"publicationDate":"2019-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvz008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37323265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 44
Mature sperm small-RNA profile in the sparrow: implications for transgenerational effects of age on fitness. 麻雀成熟精子的小 RNA 图谱:年龄对体能的跨代影响。
IF 4.8 Q1 GENETICS & HEREDITY Pub Date : 2019-05-21 eCollection Date: 2019-04-01 DOI: 10.1093/eep/dvz007
Wayo Matsushima, Kristiana Brink, Julia Schroeder, Eric A Miska, Katharina Gapp

Mammalian sperm RNA has recently received a lot of interest due to its involvement in epigenetic germline inheritance. Studies of epigenetic germline inheritance have shown that environmental exposures can induce effects in the offspring without altering the DNA sequence of germ cells. Most mechanistic studies were conducted in laboratory rodents and C.elegans while observational studies confirm the phenotypic phenomenon in wild populations of humans and other species including birds. Prominently, paternal age in house sparrows affects offspring fitness, yet the mechanism is unknown. This study provides a first reference of house sparrow sperm small RNA as an attempt to uncover their role in the transmission of the effects of paternal age on the offspring. In this small-scale pilot, we found no statistically significant differences between miRNA and tRNA fragments in aged and prime sparrow sperm. These results indicate a role of other epigenetic information carriers, such as distinct RNA classes, RNA modifications, DNA methylation and retained histones, and a clear necessity of future studies in wild populations.

哺乳动物精子 RNA 近来受到广泛关注,因为它参与了表观遗传的种系遗传。对表观遗传种系遗传的研究表明,环境暴露可在不改变生殖细胞 DNA 序列的情况下对后代产生影响。大多数机理研究都是在实验室啮齿动物和文鸟中进行的,而观察性研究则证实了人类和其他物种(包括鸟类)野生种群中的表型现象。其中最突出的是,家雀父亲的年龄会影响后代的体质,但其机制尚不清楚。本研究首次参考了家雀精子小 RNA,试图揭示其在父系年龄对后代影响的传递过程中的作用。在这项小规模试验中,我们发现老龄麻雀精子和壮年麻雀精子中的miRNA和tRNA片段在统计学上没有显著差异。这些结果表明了其他表观遗传信息载体的作用,如不同的RNA类别、RNA修饰、DNA甲基化和保留组蛋白,以及未来在野生种群中进行研究的明确必要性。
{"title":"Mature sperm small-RNA profile in the sparrow: implications for transgenerational effects of age on fitness.","authors":"Wayo Matsushima, Kristiana Brink, Julia Schroeder, Eric A Miska, Katharina Gapp","doi":"10.1093/eep/dvz007","DOIUrl":"10.1093/eep/dvz007","url":null,"abstract":"<p><p>Mammalian sperm RNA has recently received a lot of interest due to its involvement in epigenetic germline inheritance. Studies of epigenetic germline inheritance have shown that environmental exposures can induce effects in the offspring without altering the DNA sequence of germ cells. Most mechanistic studies were conducted in laboratory rodents and <i>C.elegans</i> while observational studies confirm the phenotypic phenomenon in wild populations of humans and other species including birds. Prominently, paternal age in house sparrows affects offspring fitness, yet the mechanism is unknown. This study provides a first reference of house sparrow sperm small RNA as an attempt to uncover their role in the transmission of the effects of paternal age on the offspring. In this small-scale pilot, we found no statistically significant differences between miRNA and tRNA fragments in aged and prime sparrow sperm. These results indicate a role of other epigenetic information carriers, such as distinct RNA classes, RNA modifications, DNA methylation and retained histones, and a clear necessity of future studies in wild populations.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"5 2","pages":"dvz007"},"PeriodicalIF":4.8,"publicationDate":"2019-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37286782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Environmental Epigenetics Update and Boards. 环境表观遗传学更新和董事会。
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-05-14 eCollection Date: 2019-01-01 DOI: 10.1093/eep/dvz006
Michael K Skinner
Environmental Epigenetics, an Oxford University Press publication, just initiated its fifth year of operations with this Volume 5 Issue 1. We are a completely Open Access journal listed in PMC and PubMed, along with numerous other access sites. Environmental Epigenetics initiated its review to obtain an impact factor this year. Two special issues are planned this year in Epigenetics, Environment and Reproduction and in Epigenetic Transgenerational Inheritance, both associated with corresponding scientific meetings around the world. The amount and diversity of our published studies is increasing as the field of environmental epigenetics grows and expands. We are looking forward to another productive year and encourage you to consider submissions to Environmental Epigenetics. The heart of the journal has always been the quality and breadth of the Environmental Epigenetics Editorial Boards. This Editorial is designed to extend a profound thanks to the Editorial Boards. From the beginning the Editorial Board, Consulting Editorial Board, and Editorial Review Board have provided advice and insights into the development and operations of the journal and its review process. I personally want to thank all those Editors that have acted as managing editors and reviewers. Without the dedication, experience and hard work of the Editorial Boards the journal would not exist. The current members of each of the Boards are listed below. You will see an outstanding group of dedicated and hardworking individuals that truly are the backbone of Environmental Epigenetics.
{"title":"Environmental Epigenetics Update and Boards.","authors":"Michael K Skinner","doi":"10.1093/eep/dvz006","DOIUrl":"https://doi.org/10.1093/eep/dvz006","url":null,"abstract":"Environmental Epigenetics, an Oxford University Press publication, just initiated its fifth year of operations with this Volume 5 Issue 1. We are a completely Open Access journal listed in PMC and PubMed, along with numerous other access sites. Environmental Epigenetics initiated its review to obtain an impact factor this year. Two special issues are planned this year in Epigenetics, Environment and Reproduction and in Epigenetic Transgenerational Inheritance, both associated with corresponding scientific meetings around the world. The amount and diversity of our published studies is increasing as the field of environmental epigenetics grows and expands. We are looking forward to another productive year and encourage you to consider submissions to Environmental Epigenetics. The heart of the journal has always been the quality and breadth of the Environmental Epigenetics Editorial Boards. This Editorial is designed to extend a profound thanks to the Editorial Boards. From the beginning the Editorial Board, Consulting Editorial Board, and Editorial Review Board have provided advice and insights into the development and operations of the journal and its review process. I personally want to thank all those Editors that have acted as managing editors and reviewers. Without the dedication, experience and hard work of the Editorial Boards the journal would not exist. The current members of each of the Boards are listed below. You will see an outstanding group of dedicated and hardworking individuals that truly are the backbone of Environmental Epigenetics.","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"5 1","pages":"dvz006"},"PeriodicalIF":3.8,"publicationDate":"2019-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvz006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37258533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The rat cumulative allostatic load measure (rCALM): a new translational assessment of the burden of stress. 大鼠累积负荷测量(rCALM):一种新的应激负担的转化评估方法。
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-05-04 eCollection Date: 2019-01-01 DOI: 10.1093/eep/dvz005
J Keiko McCreary, Zachary T Erickson, Eric Paxman, Douglas Kiss, Tony Montina, David M Olson, Gerlinde A S Metz

Determinants of lifetime health are complex and emphasize the need for robust predictors of disease risk. Allostatic load (AL) has become a clinical framework to estimate the cumulative biological burden associated with chronic stress. To assist knowledge translation in the developmental origins of health and disease field, clinically valid methods for reliable AL assessment in experimental models are urgently needed. Here, we introduce the rat cumulative allostatic load measure (rCALM), as a new preclinical knowledge translation tool to assess the burden of chronic stress. First, we identified an array of stress-associated physiological markers that are particularly sensitive to hypothalamic-pituitary-adrenal axis dysregulation by ancestral prenatal stress. Second, we determined which of these markers are susceptible to an intervention by environmental enrichment (EE) to mitigate AL. The markers most responsive to stress and EE therapy were assembled to become operationalized in the rCALM. Third, the new rCALM was validated for the ability to indicate future disease risks. The results show that the rCALM estimates the burden of chronic stress and serves as a proxy to estimate stress resilience and vulnerability to disease. Using the rCALM we showed that enrichment therapy can offset the adverse health outcomes linked to a high AL. Thus, the rCALM provides a model for the development of new test strategies that facilitate knowledge translation in preclinical animal models.

终生健康的决定因素是复杂的,强调需要强有力的疾病风险预测指标。适应负荷(AL)已成为评估慢性应激相关累积生物学负担的临床框架。为了帮助健康和疾病发展起源领域的知识翻译,迫切需要临床有效的实验模型中可靠的人工智能评估方法。在这里,我们介绍了大鼠累积负荷测量(rCALM),作为一种新的临床前知识转化工具来评估慢性应激负担。首先,我们确定了一系列与压力相关的生理标记,这些标记对祖先产前压力引起的下丘脑-垂体-肾上腺轴失调特别敏感。其次,我们确定了这些标记中哪些容易受到环境富集(EE)干预的影响,以减轻AL。对压力和EE治疗最敏感的标记被组装起来,在rCALM中发挥作用。第三,新的rCALM被证实具有指示未来疾病风险的能力。结果表明,rCALM可评估慢性应激负担,并可作为评估应激恢复能力和疾病易感性的代理。利用rCALM,我们发现富集治疗可以抵消与高AL相关的不良健康结果。因此,rCALM为开发新的测试策略提供了一个模型,促进临床前动物模型的知识转化。
{"title":"The rat cumulative allostatic load measure (rCALM): a new translational assessment of the burden of stress.","authors":"J Keiko McCreary,&nbsp;Zachary T Erickson,&nbsp;Eric Paxman,&nbsp;Douglas Kiss,&nbsp;Tony Montina,&nbsp;David M Olson,&nbsp;Gerlinde A S Metz","doi":"10.1093/eep/dvz005","DOIUrl":"https://doi.org/10.1093/eep/dvz005","url":null,"abstract":"<p><p>Determinants of lifetime health are complex and emphasize the need for robust predictors of disease risk. Allostatic load (AL) has become a clinical framework to estimate the cumulative biological burden associated with chronic stress. To assist knowledge translation in the developmental origins of health and disease field, clinically valid methods for reliable AL assessment in experimental models are urgently needed. Here, we introduce the rat cumulative allostatic load measure (rCALM), as a new preclinical knowledge translation tool to assess the burden of chronic stress. First, we identified an array of stress-associated physiological markers that are particularly sensitive to hypothalamic-pituitary-adrenal axis dysregulation by ancestral prenatal stress. Second, we determined which of these markers are susceptible to an intervention by environmental enrichment (EE) to mitigate AL. The markers most responsive to stress and EE therapy were assembled to become operationalized in the rCALM. Third, the new rCALM was validated for the ability to indicate future disease risks. The results show that the rCALM estimates the burden of chronic stress and serves as a proxy to estimate stress resilience and vulnerability to disease. Using the rCALM we showed that enrichment therapy can offset the adverse health outcomes linked to a high AL. Thus, the rCALM provides a model for the development of new test strategies that facilitate knowledge translation in preclinical animal models.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"5 1","pages":"dvz005"},"PeriodicalIF":3.8,"publicationDate":"2019-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvz005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37220890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Acetaminophen use during pregnancy and DNA methylation in the placenta of the extremely low gestational age newborn (ELGAN) cohort 极低胎龄新生儿(ELGAN)队列中妊娠期对乙酰氨基酚的使用和胎盘DNA甲基化
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-04-01 DOI: 10.1093/eep/dvz010
Kezia A Addo, C. Bulka, Radhika Dhingra, Hudson P. Santos, L. Smeester, T. O’Shea, Rebecca C. Fry
Abstract Acetaminophen is considered the safest antipyretic and analgesic medication for pregnant women. However, studies have reported that acetaminophen has endocrine disrupting properties and prenatal exposure has been associated with early life epigenetic changes and later life health outcomes. As the placenta is the central mediator of maternal and fetal interactions, exposure to acetaminophen during pregnancy could manifest as perturbations in the placenta epigenome. Here, we evaluated epigenome-wide cytosine-guanine dinucleotide (CpG) methylation in placental tissue in relation to maternal acetaminophen use during pregnancy in a cohort of 286 newborns born prior to 28 weeks gestation. According to maternal self-report, more than half (166 of 286) of the newborns were exposed to acetaminophen in utero. After adjustment for potential confounders, a total of 42 CpGs were identified to be differentially methylated at a false discovery rate < 0.05, with most displaying increased methylation as it relates to acetaminophen exposure. A notable gene that was significantly associated with acetaminophen is the prostaglandin receptor (PTGDR) which plays an essential role in mediating placental blood flow and fetal growth. Moreover, for 6 of the 42 CpGs, associations of acetaminophen use with methylation were significantly different between male and female placentas; 3 CpG sites were associated with acetaminophen use in the male placenta and 3 different sites were associated with acetaminophen use in the female placenta (Pinteraction < 0.2). These findings highlight a relationship between maternal acetaminophen use during pregnancy and the placental epigenome and suggest that the responses for some CpG sites are sex dependent.
对乙酰氨基酚被认为是孕妇最安全的解热镇痛药物。然而,有研究报告说,对乙酰氨基酚具有内分泌干扰特性,产前暴露与生命早期表观遗传变化和生命后期健康结果有关。由于胎盘是母体和胎儿相互作用的中心介质,在怀孕期间暴露于对乙酰氨基酚可能表现为胎盘表观基因组的扰动。在这里,我们评估了286名妊娠前28周出生的新生儿妊娠期间母体对乙酰氨基酚使用与胎盘组织全表观基因组胞嘧啶-鸟嘌呤二核苷酸(CpG)甲基化的关系。根据产妇自我报告,286名新生儿中超过一半(166名)在子宫内接触过对乙酰氨基酚。在对潜在混杂因素进行调整后,共有42个CpGs被鉴定为差异甲基化,错误发现率< 0.05,其中大多数显示甲基化增加,因为它与对乙酰氨基酚暴露有关。前列腺素受体(PTGDR)是一个与对乙酰氨基酚显著相关的基因,它在介导胎盘血流和胎儿生长中起着重要作用。此外,对于42个CpGs中的6个,对乙酰氨基酚使用与甲基化的关联在男性和女性胎盘之间存在显著差异;3个CpG位点与雄性胎盘中对乙酰氨基酚的使用相关,3个不同位点与雌性胎盘中对乙酰氨基酚的使用相关(p互作< 0.2)。这些发现强调了怀孕期间母亲对乙酰氨基酚的使用与胎盘表观基因组之间的关系,并表明一些CpG位点的反应是性别依赖的。
{"title":"Acetaminophen use during pregnancy and DNA methylation in the placenta of the extremely low gestational age newborn (ELGAN) cohort","authors":"Kezia A Addo, C. Bulka, Radhika Dhingra, Hudson P. Santos, L. Smeester, T. O’Shea, Rebecca C. Fry","doi":"10.1093/eep/dvz010","DOIUrl":"https://doi.org/10.1093/eep/dvz010","url":null,"abstract":"Abstract Acetaminophen is considered the safest antipyretic and analgesic medication for pregnant women. However, studies have reported that acetaminophen has endocrine disrupting properties and prenatal exposure has been associated with early life epigenetic changes and later life health outcomes. As the placenta is the central mediator of maternal and fetal interactions, exposure to acetaminophen during pregnancy could manifest as perturbations in the placenta epigenome. Here, we evaluated epigenome-wide cytosine-guanine dinucleotide (CpG) methylation in placental tissue in relation to maternal acetaminophen use during pregnancy in a cohort of 286 newborns born prior to 28 weeks gestation. According to maternal self-report, more than half (166 of 286) of the newborns were exposed to acetaminophen in utero. After adjustment for potential confounders, a total of 42 CpGs were identified to be differentially methylated at a false discovery rate < 0.05, with most displaying increased methylation as it relates to acetaminophen exposure. A notable gene that was significantly associated with acetaminophen is the prostaglandin receptor (PTGDR) which plays an essential role in mediating placental blood flow and fetal growth. Moreover, for 6 of the 42 CpGs, associations of acetaminophen use with methylation were significantly different between male and female placentas; 3 CpG sites were associated with acetaminophen use in the male placenta and 3 different sites were associated with acetaminophen use in the female placenta (Pinteraction < 0.2). These findings highlight a relationship between maternal acetaminophen use during pregnancy and the placental epigenome and suggest that the responses for some CpG sites are sex dependent.","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvz010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45146576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Pregnancy lipidomic profiles and DNA methylation in newborns from the CHAMACOS cohort. CHAMACOS队列中新生儿的妊娠脂质组学特征和DNA甲基化。
IF 3.8 Q1 GENETICS & HEREDITY Pub Date : 2019-04-01 eCollection Date: 2019-01-01 DOI: 10.1093/eep/dvz004
Gwen Tindula, Douglas Lee, Karen Huen, Asa Bradman, Brenda Eskenazi, Nina Holland

Lipids play a role in many biological functions and the newly emerging field of lipidomics aims to characterize the varying classes of lipid molecules present in biological specimens. Animal models have shown associations between maternal dietary supplementation with fatty acids during pregnancy and epigenetic changes in their offspring, demonstrating a mechanism through which prenatal environment can affect outcomes in children; however, data on maternal lipid metabolite levels during pregnancy and newborn DNA methylation in humans are sparse. In this study, we assessed the relationship of maternal lipid metabolites measured in the blood from pregnant women with newborn DNA methylation profiles in the Center for the Health Assessment of Mothers and Children of Salinas cohort. Targeted metabolomics was performed by selected reaction monitoring liquid chromatography and triple quadrupole mass spectrometry to measure 92 metabolites in plasma samples of pregnant women at ∼26 weeks gestation. DNA methylation was assessed using the Infinium HumanMethylation 450K BeadChip adjusting for cord blood cell composition. We uncovered numerous false discovery rate significant associations between maternal metabolite levels, particularly phospholipid and lysolipid metabolites, and newborn methylation. The majority of the observed relationships were negative, suggesting that higher lipid metabolites during pregnancy are associated with lower methylation levels at genes related to fetal development. These results further elucidate the complex relationship between early life exposures, maternal lipid metabolites, and infant epigenetic status.

脂质在许多生物功能中发挥作用,新兴的脂质组学领域旨在表征生物标本中存在的不同类别的脂质分子。动物模型显示,母亲在怀孕期间膳食中补充脂肪酸与其后代的表观遗传变化之间存在关联,证明了产前环境可以影响儿童结局的机制;然而,关于怀孕期间母体脂质代谢物水平和人类新生儿DNA甲基化的数据很少。在这项研究中,我们评估了Salinas母亲和儿童健康评估中心队列中孕妇血液中测量的母体脂质代谢物与新生儿DNA甲基化谱的关系。通过选择反应监测液相色谱和三重四极杆质谱法进行靶向代谢组学,测量妊娠~ 26周孕妇血浆样品中的92种代谢物。使用Infinium HumanMethylation 450K BeadChip调整脐带血细胞组成来评估DNA甲基化。我们发现了许多错误的发现率在母体代谢物水平,特别是磷脂和溶脂代谢物水平和新生儿甲基化之间存在显著关联。大多数观察到的关系是负的,这表明怀孕期间较高的脂质代谢物与胎儿发育相关基因的较低甲基化水平相关。这些结果进一步阐明了早期生活暴露、母亲脂质代谢产物和婴儿表观遗传状态之间的复杂关系。
{"title":"Pregnancy lipidomic profiles and DNA methylation in newborns from the CHAMACOS cohort.","authors":"Gwen Tindula,&nbsp;Douglas Lee,&nbsp;Karen Huen,&nbsp;Asa Bradman,&nbsp;Brenda Eskenazi,&nbsp;Nina Holland","doi":"10.1093/eep/dvz004","DOIUrl":"https://doi.org/10.1093/eep/dvz004","url":null,"abstract":"<p><p>Lipids play a role in many biological functions and the newly emerging field of lipidomics aims to characterize the varying classes of lipid molecules present in biological specimens. Animal models have shown associations between maternal dietary supplementation with fatty acids during pregnancy and epigenetic changes in their offspring, demonstrating a mechanism through which prenatal environment can affect outcomes in children; however, data on maternal lipid metabolite levels during pregnancy and newborn DNA methylation in humans are sparse. In this study, we assessed the relationship of maternal lipid metabolites measured in the blood from pregnant women with newborn DNA methylation profiles in the Center for the Health Assessment of Mothers and Children of Salinas cohort. Targeted metabolomics was performed by selected reaction monitoring liquid chromatography and triple quadrupole mass spectrometry to measure 92 metabolites in plasma samples of pregnant women at ∼26 weeks gestation. DNA methylation was assessed using the Infinium HumanMethylation 450K BeadChip adjusting for cord blood cell composition. We uncovered numerous false discovery rate significant associations between maternal metabolite levels, particularly phospholipid and lysolipid metabolites, and newborn methylation. The majority of the observed relationships were negative, suggesting that higher lipid metabolites during pregnancy are associated with lower methylation levels at genes related to fetal development. These results further elucidate the complex relationship between early life exposures, maternal lipid metabolites, and infant epigenetic status.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"5 1","pages":"dvz004"},"PeriodicalIF":3.8,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/eep/dvz004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37130150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
期刊
Environmental Epigenetics
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1