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Fracture Resistance of Space Maintainers Produced Using 3D Printable Materials. 使用三维打印材料制作的空间维持器的抗断裂性。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-09-01 DOI: 10.23804/ejpd.2024.2104
N Aktaş, D Atabek

Aim: This study aimed to evaluate the fracture resistance of space maintainers (SMs) produced using 3D-printable materials (metal, resin and polyetheretherketone [PEEK]) after thermal aging and compare them with conventional space maintainers.

Methods: A standardised typodont model for paediatric dentistry was utilised, and band and loop space maintainers were designed digitally using computer-aided design (CAD) technology. Four groups were established: Conventional, 3D printed metal, 3D printed resin, and 3D printed PEEK. Fracture resistance was assessed after 10,000 thermal cycles, simulating oral conditions. Fracture tests were conducted using a universal testing machine, applying vertical force to the band and loop junction until fracture. Statistical analyses were performed using one-way ANOVA and the Tukey HSD test (P<0.05).

Conclusion: Although the fracture resistance values showed that metal, resin, and PEEK 3D printed band and loop space maintainers can be acceptable clinically, the permanent resin may be preferable to printable material because of their aesthetic properties.

目的:本研究旨在评估使用三维打印材料(金属、树脂和聚醚醚酮 [PEEK])制作的间隙保持器(SMs)在热老化后的抗断裂性,并将其与传统的间隙保持器进行比较:方法:利用儿童牙科的标准化类型齿模型,使用计算机辅助设计(CAD)技术对带状和环状间隙保持器进行数字化设计。分为四组:传统组、3D 打印金属组、3D 打印树脂组和 3D 打印 PEEK 组。在模拟口腔条件下,经过 10,000 次热循环后对抗折性进行了评估。断裂测试使用万能试验机进行,在带环和环连接处施加垂直力,直至断裂。统计分析采用单因素方差分析和 Tukey HSD 检验(PConclusion):虽然抗断裂强度值表明金属、树脂和 PEEK 三维打印带环空间维持器在临床上是可以接受的,但永久性树脂可能比可打印材料更好,因为它们具有美观特性。
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引用次数: 0
Developmental defects of enamel in childhood cancer survivors: A systematic review and meta-analysis. 儿童癌症幸存者的釉质发育缺陷:系统回顾和荟萃分析。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.23804/ejpd.2024.2256
A Allam, N Camoni, S Cirio, C Salerno, G Campus, M G Cagetti

Aim: The present systematic review and meta-analysis (Prospero registration number: CRD42023472016) aims to assess the prevalence of developmental defects of enamel (DDEs), qualitatively and/or quantitatively, in childhood cancer survivors (CCS) and evaluate, when possible, these data in comparison with those found in healthy children.

Methods: Three electronic databases (PubMed, Embase, Scopus) were searched from January 2003 to January 2024 for studies reporting on DDEs in children with a mean age not exceeding 16 years at the time of the study who underwent antineoplastic therapy. The ROBINS-I and the Joanna Briggs Institute (JBI) tools were used to assess the risk of bias. Included studies with comparable outcomes underwent random effects models meta-analysis using Stata®18.

Conclusion: CCS showed a higher prevalence of DDEs, both qualitative and quantitative, compared to healthy children. The meta- analysis showed higher odds of developing qualitative defects over quantitative defects in CCS. Conclusions regarding the association between the type of therapy administered, age of therapy initiation, and prevalence of DDEs could not be drawn due to insufficient data. A lack of a standardized method of detecting enamel defects posed a challenge in the qualitative and quantitative analysis.

目的:本系统综述和荟萃分析(Prospero注册号:CRD42023472016)旨在定性和/或定量评估儿童癌症幸存者(CCS)牙釉质发育缺陷(DDEs)的发生率,并尽可能将这些数据与健康儿童的数据进行比较评估:从 2003 年 1 月到 2024 年 1 月,我们在三个电子数据库(PubMed、Embase、Scopus)中搜索了有关接受抗肿瘤治疗的平均年龄不超过 16 岁的儿童 DDEs 的研究报告。采用 ROBINS-I 和 Joanna Briggs Institute (JBI) 工具评估偏倚风险。使用 Stata®18 对纳入的具有可比结果的研究进行随机效应模型 meta 分析:结论:与健康儿童相比,儿童营养服务在定性和定量方面都显示出更高的 DDEs 患病率。元分析表明,在儿童慢性疾病患者中,出现定性缺陷的几率高于定量缺陷。由于数据不足,无法就治疗类型、开始治疗的年龄与 DDEs 患病率之间的关联得出结论。缺乏检测釉质缺陷的标准化方法给定性和定量分析带来了挑战。
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引用次数: 0
Evaluation of treatment outcomes with passive presurgical infant orthopaedics in patients with non-syndromic cleft lip and or palate: A systematic review conducted according to PRISMA guidelines and the Cochrane Handbook for Systematic Reviews of Interventions. 评估非综合征唇腭裂患者手术前婴儿被动矫形的治疗效果:根据 PRISMA 指南和 Cochrane 《干预措施系统综述手册》进行的系统综述。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.23804/ejpd.2024.2056
N Bhatia, A K Subramanian, N Ramasamy, M Di Blasio, V Ronsivalle, M Cicciù, G Minervini

Aim: The primary goal of the present comprehensive systematic review is to assess the treatment results attained through PSIO in individuals with NSCLP, as well as to explore its effectiveness. This will be accomplished by examining a range of existing published studies that focus on patient groups who have undergone PPIO.

Materials: A comprehensive search strategy was devised by a single author for each database without language restrictions. The databases searched were PubMed, Cochrane library Google Scholar, Web of Science and Scopus. The search period was limited to the years 2001 to 2023. Duration and study design filter was used. Furthermore, conference proceedings, abstracts, and reference lists of relevant studies were sought to obtain additional records.

Conclusion: The examined protocols for passive presurgical infant orthopaedics (PPIO) in individuals with CLP typically did not reveal notable treatment outcomes when compared to a lack of treatment across a range of domains. These domains encompass feeding patterns, facial aesthetics, maxillary dentoalveolar factors, cephalometric measurements, speech and language-related aspects, dental arch alignments, economic assessments, as well as occurrences of adverse effects or complications associated with applied appliances or procedures.

目的:本综合系统综述的主要目的是评估PSIO对NSCLP患者的治疗效果,并探讨其有效性。为了实现这一目标,我们将对已发表的一系列以接受过PPIO治疗的患者群体为重点的研究进行审查:由一位作者为每个数据库设计了一个综合搜索策略,没有语言限制。搜索的数据库包括 PubMed、Cochrane 图书馆、Google Scholar、Web of Science 和 Scopus。搜索时间限于 2001 年至 2023 年。使用了时间和研究设计过滤器。此外,还查找了相关研究的会议记录、摘要和参考文献列表,以获得更多记录:结论:所研究的针对CLP患者的婴儿被动术前矫形(PPIO)方案在一系列领域中与缺乏治疗相比,通常没有显示出显著的治疗效果。这些领域包括喂养模式、面部美学、上颌牙槽因素、头颅测量、言语和语言相关方面、牙弓排列、经济评估以及与应用矫治器或程序相关的不良反应或并发症的发生。
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引用次数: 0
Upper Jaw Development After Lingual Laser Frenectomy and Myofuntional Therapy. 舌侧激光切除术和肌肉治疗后的上颌骨发育
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.23804/ejpd.2024.2143
L De Simone, D Martinelli, M Tieri, A Monaco, D Tripodi

Aim: Over the years, the role of the lingual frenulum in the development of skeletal structures has been evaluated. However, the effect of short lingual frenulum treatment on bone structures is unclear. We need to investigate the effect of surgery and myofunctional therapy on maxillary development. This could be the rationale to establish an orthodontic interception protocol.

Methods: The case-control study was carried out in the Operative Unit of Pedodontics of the University Dental Clinic, at the Department of Medical, Oral and Biotechnological Sciences of the University of "G. D'Annunzio" Chieti-Pescara. The study population consisted in thirty children. The data were collected through anamnesis carried out by qualified health personnel before dental examination. Photos, frenulum length and cast measurements were taken. Student's t-test statistical analysis was used. Significance was ascertained based on the obtained P value of less than 0.01.

Conclusion: The results confirmed the benefit of laser surgical treatment. The change in parameters related to the anterior palate region showed the power of frenectomy and myofunctional therapy in restoring the tongue's ability to stimulate bone development. The protocol adopted in the following study could be used in interceptive orthodontics. However, further studies are needed to evaluate the effects on the upper jaw over the long term that have higher sample numbers.

目的:多年来,人们一直在评估舌侧蹼在骨骼结构发育中的作用。然而,短舌裂治疗对骨骼结构的影响尚不明确。我们需要研究手术和肌功能治疗对上颌骨发育的影响。这可能是制定正畸阻断方案的依据:病例对照研究在基耶蒂-佩斯卡拉 "G. D'Annunzio "大学医学、口腔和生物技术科学系大学牙科诊所的儿童牙科手术室进行。研究对象包括 30 名儿童。数据由合格的医务人员在牙科检查前通过肛门指诊收集。照片、瓣膜长度和石膏测量均已完成。采用学生 t 检验进行统计分析。以 P 值小于 0.01 为差异有显著性:结果证实了激光手术治疗的益处。腭前区相关参数的变化显示了切除术和肌功能疗法在恢复舌刺激骨发育能力方面的作用。以下研究中采用的方案可用于阻断性正畸。不过,还需要进行更多的研究,以评估对上颌骨的长期影响。
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引用次数: 0
Interdisciplinary management of orofacial lesions in early childhood: paediatric alert system at University of Verona. 幼儿口面部损伤的跨学科管理:维罗纳大学的儿科警报系统。
IF 3.6 2区 医学 Q1 Medicine Pub Date : 2024-06-03 Epub Date: 2024-05-01 DOI: 10.23804/ejpd.2024.2141
N Zerman, A Zangani, C Maffeis, A Pietrobelli, G Piacentini, F Zotti, R De Manzoni, R Silva, A Cordiano, E Spinas, P F Nocini

Background: Orofacial trauma (OFT) occurs frequently in children and requires thorough evaluation not only by paediatric dentists but also by all specialists involved in emergency services, particularly in cases involving children under 3 years of age, given their inability to directly participate in clinical-anamnestic evaluations. Addressing early childhood orofacial trauma resulting from maltreatment, this study explores the key role played by various healthcare professionals, including paediatric dentists, general dentists, maxillofacial surgeons, dental hygienists, and paediatricians, in the optimal management of these cases. In the event of trauma due to suspected or confirmed mistreatment, it is essential that all healthcare workers involved have precise knowledge of the appropriate course of action from both a clinical and legal point of view, guaranteeing maximum protection for the young patient. This is particularly significant as cases of mistreatment with apparently minor consequences can degenerate into situations of irreparable severity. The latest guidelines from the International Association of Dental Traumatology (IADT) in 2020 continue to emphasise the potential correlation between OFT and cases of abuse or violence. Recent recommendations in the literature highlight the importance of facilitating mandatory reporting of incidents to relevant authorities and improving information sharing between dental healthcare professionals and child welfare services. A new flow diagram, called Paediatric Orofacial Trauma Alert (P.O.T.A.), has been proposed at the University of Verona. This tool is specifically designed to assist specialists dealing with early childhood orofacial trauma cases by assisting them in identifying potential cases of maltreatment. In this innovative approach, the collaborative efforts of general dentists, paediatric dentists, maxillofacial surgeons, dental hygienists and paediatricians play a vital role in cases of abuse. In addition to restoring the oral health of young patients, these professionals can activate a vast network of contacts, ensuring not only optimal oral health care but also providing comprehensive support to victims. The objective is to safeguard not only the physical but also the psychological well-being of these vulnerable subjects.

背景:颌面外伤(OFT)经常发生在儿童身上,不仅需要儿科牙医进行全面评估,还需要所有参与急救服务的专家进行全面评估,尤其是涉及 3 岁以下儿童的病例,因为他们无法直接参与临床-肛门内科评估。针对虐待导致的幼儿口面部创伤,本研究探讨了包括儿童牙医、普通牙医、颌面外科医生、牙科卫生员和儿科医生在内的各种医疗专业人员在这些病例的最佳治疗过程中发挥的关键作用。在发生疑似或已证实的虐待造成的创伤时,所有相关医护人员都必须准确掌握从临床和法律角度出发的适当行动方案,以确保为年幼患者提供最大程度的保护。这一点尤为重要,因为看似后果轻微的虐待案件可能会恶化成无法弥补的严重后果。国际牙科创伤学会(IADT)在 2020 年发布的最新指南中继续强调了口腔外伤与虐待或暴力之间的潜在关联。最近的文献建议强调了促进向相关机构强制报告事件以及改善牙科医疗保健专业人员与儿童福利服务机构之间信息共享的重要性。维罗纳大学(University of Verona)提出了一种名为 "儿童口面部创伤警报"(P.O.T.A.)的新流程图。该工具专门用于协助专家处理幼儿口面部创伤病例,帮助他们识别潜在的虐待病例。在这一创新方法中,普通牙医、儿童牙医、颌面外科医生、牙科卫生员和儿科医生的共同努力在虐待病例中发挥了至关重要的作用。除了恢复年轻患者的口腔健康外,这些专业人员还可以启动一个庞大的联系网络,不仅确保最佳的口腔保健,还为受害者提供全面的支持。这样做的目的不仅是为了保护这些弱势群体的身体健康,也是为了保护他们的心理健康。
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引用次数: 0
Dog-Assisted Therapy (DAT) for the management of anxiety during paediatric dental care. A scoping review. 狗辅助治疗(DAT)用于缓解儿科牙科护理过程中的焦虑。范围综述。
IF 3.6 2区 医学 Q1 Medicine Pub Date : 2024-06-03 Epub Date: 2024-03-01 DOI: 10.23804/ejpd.2024.2001
A Garrocho-Rangel, M Rosales-Bérber, D Ríos-Méndez, J Flores-Velázquez, D Trejo-Herbert, F Pozos-Guillén, A Pozos-Guillén

Aim: Distraction techniques in paediatric dentistry can be effective in decreasing the child's attention span from an unpleasant or stressful procedure. Distraction is achieved through imagination, audio, and/or visual stimuli. It has been shown that the accompaniment and participation of animals or pets, specifically dogs (Dog-Assisted Therapy or DAT), during medical, oral, and therapeutic activities can improve the physical and mental health of patients, especially children. However, there is limited information available regarding the impact of incorporating a certified therapy dog into the paediatric dental environment as a distraction strategy to alleviate anxiety levels during dental procedures.

Methods: This scoping review aimed to identify and review published articles concerning the use of DAT in paediatric dentistry. The article discusses indications, benefits, and potential risks to human health and safety in clinical settings. Eligible sources encompass clinical trials, observational studies, and narrative reviews written in either English or Spanish and published over the last two decades, sourced from four electronic databases. Ultimately, seven pertinent studies were included in the review.

Conclusion: DAT presents itself as a promising alternative in managing anxiety and stress among children during dental visits. The integration of a therapy dog and its handler into the paediatric oral care team should be thoughtfully considered by clinicians as a means to enhance the comfort and compliance of apprehensive patients.

目的:儿童牙科中的分散注意力技术可以有效地减少儿童对不愉快或紧张程序的注意力。分散注意力可以通过想象、声音和/或视觉刺激来实现。有研究表明,在医疗、口腔和治疗活动中,动物或宠物(特别是狗(狗辅助治疗或 DAT))的陪伴和参与可以改善患者(尤其是儿童)的身心健康。然而,关于在儿科牙科治疗环境中使用经认证的治疗犬作为分散注意力的策略以减轻牙科治疗过程中的焦虑水平所产生的影响,目前可获得的信息还很有限:本范围综述旨在确定和综述已发表的有关在儿童牙科中使用 DAT 的文章。文章讨论了在临床环境中使用 DAT 的适应症、益处以及对人体健康和安全的潜在风险。符合条件的资料来源包括用英语或西班牙语撰写的临床试验、观察性研究和叙述性综述,这些资料都是在过去二十年中发表的,来源于四个电子数据库。最终,七项相关研究被纳入综述:结论:DAT 是治疗儿童看牙期间的焦虑和压力的一种很有前景的替代方法。临床医生应深思熟虑地考虑将治疗犬及其训导员纳入儿科口腔护理团队,以此提高焦虑患者的舒适度和依从性。
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引用次数: 0
Different effects of whitening treatment on natural teeth and teeth treated with Icon resin: an experimental study in vitro. 美白处理对天然牙齿和用 Icon 树脂处理过的牙齿的不同影响:体外实验研究。
IF 3.6 2区 医学 Q1 Medicine Pub Date : 2024-06-03 Epub Date: 2024-03-01 DOI: 10.23804/ejpd.2024.2014
R Favero, M Pellarin, D Bresolato, A Di Fiore, A Zuccon, A Volpato, L Favero

Background: It is quite common for white spots to develop on a tooth, due sometimes to a defective formation of the enamel layer, and sometimes to patches of demineralisation as a result of poor oral hygiene during orthodontic treatment with fixed braces. ICON DMG is currently the only noninvasive treatment for white spots. After a preliminary etching, it infiltrates the enamel, filling the spaces between the prisms with a resinous material that has a refraction coefficient very similar to that of healthy tooth enamel. The aim of this study was to test the efficacy of professional whitening procedures on teeth previously treated with ICON. The study hypothesis was that infiltration with ICON resin creates a barrier capable of preventing the bleaching action of the whitening agent.

Materials: White spots were artificially created on one half of the vestibular surface of 12 human teeth, while the other half was protected with a composite adhesive. The white spots were infiltrated with ICON and the protective adhesive was subsequently removed. A professional teeth whitening procedure was then completed on both halves of the teeth. A statistical analysis was performed to compare spectrophotometric recordings obtained before and after the ICON infiltration and teeth whitening procedures.

Conclusion: The whitening procedure modified the colour of the teeth on the half not infiltrated with ICON (p<0.05), but there was no statistically significant change in colour on the half infiltrated with ICON. The presence of the ICON resin seems to act as a partial barrier to the action of the whitening agent.

背景:牙齿上出现白斑是很常见的现象,有时是由于釉质层的形成缺陷,有时是由于固定矫治器正畸治疗期间口腔卫生不良导致的脱矿斑。ICON DMG 是目前治疗白斑的唯一非侵入性疗法。在初步蚀刻后,它渗入珐琅质,用一种树脂材料填充棱镜之间的空隙,这种材料的折射系数与健康牙齿珐琅质的折射系数非常相似。这项研究的目的是测试专业美白程序对曾用 ICON 治疗过的牙齿的功效。研究假设是,ICON 树脂的浸润会形成一层屏障,能够阻止美白剂的漂白作用:材料:在 12 颗人类牙齿前庭表面的一半人为制造白斑,另一半用复合粘合剂保护。用 ICON 对白斑进行浸润,然后去除保护粘合剂。然后在两半牙齿上完成专业牙齿美白程序。对 ICON 渗入和牙齿美白程序前后的分光光度记录进行了统计分析比较:结论:美白程序改变了未浸润 ICON 的那一半牙齿的颜色(p
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引用次数: 0
Humanisation and health needs in children: present or future? 人性化与儿童的健康需求:现在还是未来?
IF 3.6 2区 医学 Q1 Medicine Pub Date : 2024-06-03 Epub Date: 2024-02-01 DOI: 10.23804/ejpd.2024.2012
A V Brescia, A Mastroianni, G Parisi, G Di Gennaro, R Docimo, L Montesani

Aim: For a few years, teledentistry has been an emerging innovative strategy with potential in the field of paediatric dentistry. There are still few studies in this regard, so further research is needed to verify and ensure that teledentistry is not only an accessible mode of communication, but above all effective and evidence-based. This study aimed to use a preliminary telematic approach to promote the compliance of patients in the developmental age during the first dental visit.

Materials: Two hundred patients were selected according to the eligibility criteria, and distributed in two groups: a study group with the preliminary telematic approach (ATP) before the first visit and a control group with traditional first visit without ATP. Through an ordinal semi-proportional regression model, the degrees of collaboration between the study and control groups were compared, correcting the estimate for age groups, the presence of systemic pathologies, disorders of cognition, attention and learning, degree of anxiety and previous medical-dental experiences.

Conclusion: The preliminary telematic approach could be useful as a support to the traditional paediatric dental visit, to promote better management and fidelity of the patient, reducing anxiety and increasing collaboration during the first visit.

目的:几年来,远程牙科治疗一直是儿童牙科领域一种新兴的、具有潜力的创新策略。但这方面的研究仍然很少,因此需要进一步的研究来验证并确保远程牙科治疗不仅是一种方便的交流方式,而且是有效的、有实证依据的。本研究旨在使用一种初步的远程医疗方法来促进发育期患者在首次牙科就诊时的依从性:材料:根据资格标准选取了 200 名患者,并将其分为两组:在首次就诊前使用初步远程信息处理方法(ATP)的研究组和不使用 ATP 的传统首次就诊对照组。通过一个序数半比例回归模型,比较了研究组和对照组之间的合作程度,并根据年龄组、是否存在系统性疾病、认知、注意力和学习障碍、焦虑程度以及以往的医疗牙科经验对估计值进行了修正:初步的远程医疗方法可作为传统儿童牙科就诊的辅助手段,促进更好地管理和忠实于患者,减少焦虑,加强首次就诊期间的合作。
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引用次数: 0
Evaluation of two different remineralising toothpastes in children with drug-controlled asthma and allergic rhinitis: a randomised clinical trial. 评估药物控制哮喘和过敏性鼻炎患儿使用的两种不同再矿化牙膏:随机临床试验。
IF 3.6 2区 医学 Q1 Medicine Pub Date : 2024-06-03 Epub Date: 2024-02-01 DOI: 10.23804/ejpd.2024.2130
A Scribante, M Pascadopoli, P Bergomi, A Licari, G L Marseglia, F M Bizzi, A Butera

Aim: To compare the efficacy of two remineralising toothpastes in children suffering from asthma and allergic rhinitis after a 6-month study.

Methods: 40 patients aged between 6-14 years with enamel demineralisations were enrolled for the study at the Unit of Dental Hygiene of the University of Pavia (Italy). The following indices were collected: Schiff air index (SAI), plaque index (PI), bleeding on probing (BoP), salivary pH, Basic Erosive Wear Examination (BEWE), susceptibility index (SI) for hard and soft tissues' pathologies, and decayed missing filled teeth (DMFT). After mechanical debridement with piezoelectric instrumentation and glycine powder, patients were equally divided into two groups: Group 1 using a toothpaste with zinc hydroxyapatite, and Group 2 using a toothpaste containing calcium sodium phosphosilicate. The toothpaste had to be used twice a day. The time frames of the study were: baseline (T0), after 1 month (T1), after 3 months (T2), after 6 months (T3).

Conclusion: The tested toothpastes can be proposed for home use in children with asthma and allergic rhinitis as they significantly reduced dental sensitivity and periodontal indices.

目的:在为期 6 个月的研究后,比较两种再矿化牙膏对哮喘和过敏性鼻炎患儿的疗效。方法:意大利帕维亚大学牙科卫生室招募了 40 名年龄在 6-14 岁之间的牙釉质脱矿患者参与研究。收集了以下指数希夫空气指数 (SAI)、牙菌斑指数 (PI)、探诊出血量 (BoP)、唾液 pH 值、基本腐蚀磨损检查 (BEWE)、软硬组织病变易感性指数 (SI) 以及龋坏缺失牙 (DMFT)。使用压电仪器和甘氨酸粉进行机械清创后,将患者平均分为两组:第一组使用含羟基磷灰石锌的牙膏,第二组使用含磷硅酸钙钠的牙膏。牙膏必须每天使用两次。研究的时间范围为:基线(T0)、1 个月后(T1)、3 个月后(T2)、6 个月后(T3):建议哮喘和过敏性鼻炎患儿在家中使用测试的牙膏,因为它们能显著降低牙齿敏感性和牙周指数。
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引用次数: 0
Silver diamine fluoride: A stem cell cytotoxicity and dentin growth factor release in-vitro study. 二胺氟化银:一项干细胞细胞毒性和牙本质生长因子释放的体外研究。
IF 3.6 2区 医学 Q1 Medicine Pub Date : 2024-06-03 Epub Date: 2023-06-01 DOI: 10.23804/ejpd.2023.1928
M M Khalil, A Bashir, Y Monjazebi, M Shah, A R Tawfik, I Hussein, M Jamal

Aim: To investigate the effect of 38% SDF and its serial dilutions on the Stem cells from Human Exfoliated Deciduous teeth (SHED) and its ability to release growth factors from deciduous dentine.

Methods: The viability of SHED post-exposure to 38%, 3.8%, 0.38%, 0.038%, and 0.0038% SDF were assessed at 2, 5, and 7 days using the CyQuant assay, and results were validated using the MTT assay. The osteogenic differentiation of the cells was also investigated post-exposure to 0.0038% SDF. The release of the growth factors; TGF-β1, FGF-b, BMP-2, and VEGF from deciduous dentin discs exposed to 38% SDF, 0.0038% SDF, Ca(OH)2, MTA, and 17% EDTA were examined using ELISA. Statistical analysis was performed using means and standard deviations (p < 0.05). Two-way ANOVA compared the means of more than two groups with Tukey's multiple comparison test. The unpaired t-test was also used to compare the differences between the two data sets.

Conclusion: 38% SDF released dentinogenic growth factors from dentin discs, potentially explaining its role in reactionary dentinogenesis. Moreover, 0.0038% SDF resulted in a non-cytotoxic concentration that enhanced cellular proliferation and released bioactive molecules from dentin comparable to the 38% concentration. After further investigations, the 0.0038% dilution of SDF could present itself as a clinical concentration.

目的:研究 38% SDF 及其系列稀释液对人类脱落牙齿(SHED)干细胞的影响及其从脱落牙本质中释放生长因子的能力:方法:使用 CyQuant 检测法评估暴露于 38%、3.8%、0.38%、0.038% 和 0.0038% SDF 后的 SHED 在 2、5 和 7 天的存活率,并使用 MTT 检测法验证结果。在暴露于 0.0038% SDF 后,还对细胞的成骨分化进行了研究。使用 ELISA 检测了暴露于 38% SDF、0.0038% SDF、Ca(OH)2、MTA 和 17% EDTA 的脱落牙本质盘中的生长因子 TGF-β1、FGF-b、BMP-2 和 VEGF 的释放情况。统计分析采用均值和标准差(P < 0.05)。用 Tukey's 多重比较检验对两组以上的均值进行双向方差分析。结论:38% 的 SDF 能从牙本质盘释放牙本质生成生长因子,这可能解释了它在反应性牙本质生成中的作用。此外,0.0038% 的 SDF 浓度无细胞毒性,可促进细胞增殖并从牙本质中释放生物活性分子,与 38% 的浓度相当。经过进一步研究,0.0038% 的 SDF 稀释液可作为一种临床浓度。
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引用次数: 0
期刊
European journal of paediatric dentistry
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