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Rare diseases: a challenge in paediatric dentistry. 罕见疾病:儿童牙科面临的挑战。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-09-03 Epub Date: 2024-09-01 DOI: 10.23804/ejpd.2024.25.03.01
M R Giuca
<p><p>Rare diseases are an often an overlooked public health problem. Although they are infrequent, occurring on average in 100-500 people per million, these diseases represent a significant challenge in paediatric dentistry due to their complex manifestations and the need for specialised care. Conditions such as X-linked hypophosphatemic rickets (XLH), hypophosphatasia (HPP), and osteogenesis imperfecta (OI) exemplify the intersection of systemic health issues and oral health, requiring a multidisciplinary approach for their effective management. Dentists frequently play a crucial role in identifying genetic alterations through their dental manifestations and then referring patients to the geneticist for a definitive diagnosis. X-linked hypophosphatemia is the most common genetic form of rickets, with a prevalence of 1/20,000 - 1/60,000. XLH is characterised by stunted growth with disproportionate short stature, bowing of the lower limbs associated with reduced motor skills, osteoarticular pain, hypotonia, and dental and periodontal anomalies. XLH is due to inactivating mutations in the PHEX gene which cause excessive production of fibroblast growth factor 23 (FGF23). Increased concentration of FGF23 represents the main pathogenetic mechanism of XLH, stimulating urinary phosphate loss and renal 24-hydroxylase activity, and reducing renal 1α-hydroxylase activity with insufficient production of 1,25 -dihydroxy-vitamin D (1,25(OH)2D). PHEX protein is also expressed in osteoblasts, osteocytes, and odontoblasts. Regardless of FGF23's systemic effects on phosphate homeostasis, odontoblast differentiation, and dentin formation, its overexpression directly reduces osteoblast differentiation and matrix mineralisation. In patients with XLH, the deficit of 1,25(OH)2D induced by FGF23 causes poor enamel mineralisation with presence of cracks on teeth surface. XLH patients have recurrent dental abscesses with fistulas. Radiographic investigations highlight a generalised enlargement of the pulp chambers, molars with short roots, and a taurodontic appearance. Hypophosphatasia (HPP) is another condition in which dental manifestations precede systemic symptoms; it is a rare genetic disease (1/300,000 for severe forms, 1/100,000 for moderate forms. The incidence is perhaps underestimated due to missed diagnosis of moderate forms of the disease). It mainly affects bone and dental mineralisation. It is caused by pathogenic variant mutations in the ALPL gene which is located on the short arm of chromosome 1 and encodes the non-tissue-specific alkaline phosphatase (TNSALP) enzyme. TNSALP deficiency results in vitamin B6 (pyridoxine) deficiency and pathological accumulation of alkaline phosphatase substrates which may be responsible for extra-osseous manifestations, such as neurologic ones (pyridoxine sensitive seizures) as well as involvement of muscles and joints (arthropathies, muscle fatigue/hypotonia). Early non-traumatic loss of primary teeth between the ages of
牙科专业人员的作用不仅限于诊断这些罕见疾病,还包括提供具体的、为患者量身定制的治疗,鼓励定期看牙的预防性护理,以及对患者进行教育,其最终目标不仅是促进口腔健康,而且是促进患者的整体健康。
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引用次数: 0
Children's toothbrushing: a population survey on behaviours, attitudes, and knowledge in parents of Callao, Peru. 儿童刷牙:关于秘鲁卡亚俄家长的行为、态度和知识的人口调查。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-09-01 DOI: 10.23804/ejpd.2024.2144
R Chavez-Garamonte, J Dulanto-Vargas, K Carranza-Samanez

Aim: To determine the factors associated with behaviours, attitudes and knowledge of parents about children's toothbrushing (CTB).

Methods: This is a cross-sectional analytical study of a calculated sample of 3,534 parents/guardians (92.6% female; 7.4% male; average age = 34 years [29-37]) of schoolchildren aged 3 to 8 years in 19 public schools in the province of Callao (Peru). A 31-item face-to-face/virtual questionnaire was divided into: 5 behavioural (18 (IQR)=3]; good 76%), 5 attitudes (13 [IQR=4]; negative 74.2%) and 10 knowledge (12 [IQR=4]; low 92.8%) (KMO≥0.537, Bartlett<0,001) and reliability (ω≥0.537) and 11 demographic domains. Bivariate and multivariate linear Poisson models were used with a P<0.05.

Conclusion: Children of parents with a higher education showed good behaviours related to CTB. Negative parental attitudes and low knowledge about CTB were associated with a lower parental education and toothbrushing frequency.

目的:确定与家长对儿童刷牙(CTB)的行为、态度和知识相关的因素:这是一项横断面分析研究,研究对象是卡亚俄省(秘鲁)19 所公立学校中 3 至 8 岁学龄儿童的 3,534 名家长/监护人(92.6% 为女性;7.4% 为男性;平均年龄为 34 岁 [29-37])。面对面/虚拟问卷共 31 个项目,分为5项行为(18(IQR=3];良好76%)、5项态度(13[IQR=4];负面74.2%)和10项知识(12[IQR=4];低92.8%)(KMO≥0.537,巴特利结论:父母受过高等教育的儿童表现出与 CTB 有关的良好行为。父母对 CTB 的消极态度和低知识水平与父母教育程度和刷牙频率较低有关。
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引用次数: 0
A Systematic Review and Meta-analysis of Regenerative Pulpotomy in the Treatment of Vital Primary Teeth. 再生牙髓切断术治疗重要原牙的系统性回顾和 Meta 分析。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-09-01 DOI: 10.23804/ejpd.2024.1981
N T Snigdha, M I Karobari, A Kamarudin, N A Samsudin, F Baharin, N Dziaruddin, A A Assiry, A M Luke, G A Scardina

Aim: To assess the clinical and radiographic success rates of regenerative pulpotomy compared to devitalising or preservative pulpotomy in treating vital pulp exposure in primary teeth.

Methods: Comprehensive search with PubMed/MEDLINE, Cochrane Library, Web of Science, Google Scholar and Open Grey databases was done and two reviewers extracted the data after assessing the studies for eligibility criteria. The Cochrane Collaboration Tool and Minors Checklist assessed the quality of the selected studies. A meta-analysis was performed using RevMan (P<0.05).

Conclusion: Within the limitations of the study, it can be concluded that MTA seemed to be the better alternative to FC and FS, showing a lower risk of failure and can be recommended for pulpotomy in primary teeth. BD also demonstrated promising results as a pulpotomy medicament.

目的:评估再生牙髓切断术与去活力或防腐牙髓切断术相比,在治疗基牙牙髓暴露方面的临床和放射学成功率:方法:在PubMed/MEDLINE、Cochrane Library、Web of Science、Google Scholar和Open Grey数据库中进行了全面检索,两名审稿人在评估了研究的资格标准后提取了数据。Cochrane 协作工具和未成年人检查表对所选研究的质量进行了评估。使用 RevMan 进行了荟萃分析(PConclusion:在研究的局限性范围内,可以得出结论:MTA 似乎是 FC 和 FS 的更好替代品,显示出较低的失败风险,可推荐用于基牙的牙髓切断术。BD 作为一种牙髓切断药物也显示出了良好的效果。
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引用次数: 0
Comparison between manual vs electric powered toothbrush by using the PCR index in paediatric dentistry. 在儿童牙科中使用 PCR 指数对手动牙刷和电动牙刷进行比较。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-09-01 DOI: 10.23804/ejpd.2024.2138
F S Ludovichetti, A G Signoriello, E Trevisiol, A Gracco, E Stellini, A Zuccon, S Mazzoleni

Aim: This present study has two primary objectives: firstly, to compare the effectiveness of an electric toothbrush versus a manual toothbrush in biofilm removal; secondarily, to assess the effectiveness of individualised instructions provided by the operator.

Materials: Fifty-five paediatric patients were enrolled in the study. Data collection involved three main steps: 1) Pre-brushing PCR index collection; 2) Post-brushing PCR index collection; 3) Post-brushing PCR index collection after providing individual instructions. The tooth surfaces were colored with a liquid plaque detector. To assess whether there was a statistically significant difference in plaque values before and after brushing, a Wilcoxon signed-rank test was conducted. The Wilcoxon test with continuity correction was performed to compare the two brushing methods.

Conclusion: It is possible to confirm a correlation between instructions provided by the operator and lower plaque index. As regards the comparison between manual and electric toothbrush, in our study one tool did not emerge as more effective than the other.

目的:本研究有两个主要目的:首先,比较电动牙刷和手动牙刷在清除生物膜方面的效果;其次,评估操作员提供的个性化指导的效果:研究招募了 55 名儿科患者。数据收集包括三个主要步骤:1)刷牙前 PCR 指数收集;2)刷牙后 PCR 指数收集;3)提供个性化指导后 PCR 指数收集。使用液体牙菌斑检测器对牙齿表面进行着色。为评估刷牙前后牙菌斑值是否存在显著的统计学差异,进行了 Wilcoxon 符号秩检验。在比较两种刷牙方法时,进行了带连续性校正的 Wilcoxon 检验:结论:操作员提供的指导与牙菌斑指数降低之间存在相关性。至于手动牙刷和电动牙刷之间的比较,在我们的研究中,一种工具并不比另一种工具更有效。
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引用次数: 0
Developmental defects of enamel in childhood cancer survivors: A systematic review and meta-analysis. 儿童癌症幸存者的釉质发育缺陷:系统回顾和荟萃分析。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.23804/ejpd.2024.2256
A Allam, N Camoni, S Cirio, C Salerno, G Campus, M G Cagetti

Aim: The present systematic review and meta-analysis (Prospero registration number: CRD42023472016) aims to assess the prevalence of developmental defects of enamel (DDEs), qualitatively and/or quantitatively, in childhood cancer survivors (CCS) and evaluate, when possible, these data in comparison with those found in healthy children.

Methods: Three electronic databases (PubMed, Embase, Scopus) were searched from January 2003 to January 2024 for studies reporting on DDEs in children with a mean age not exceeding 16 years at the time of the study who underwent antineoplastic therapy. The ROBINS-I and the Joanna Briggs Institute (JBI) tools were used to assess the risk of bias. Included studies with comparable outcomes underwent random effects models meta-analysis using Stata®18.

Conclusion: CCS showed a higher prevalence of DDEs, both qualitative and quantitative, compared to healthy children. The meta- analysis showed higher odds of developing qualitative defects over quantitative defects in CCS. Conclusions regarding the association between the type of therapy administered, age of therapy initiation, and prevalence of DDEs could not be drawn due to insufficient data. A lack of a standardized method of detecting enamel defects posed a challenge in the qualitative and quantitative analysis.

目的:本系统综述和荟萃分析(Prospero注册号:CRD42023472016)旨在定性和/或定量评估儿童癌症幸存者(CCS)牙釉质发育缺陷(DDEs)的发生率,并尽可能将这些数据与健康儿童的数据进行比较评估:从 2003 年 1 月到 2024 年 1 月,我们在三个电子数据库(PubMed、Embase、Scopus)中搜索了有关接受抗肿瘤治疗的平均年龄不超过 16 岁的儿童 DDEs 的研究报告。采用 ROBINS-I 和 Joanna Briggs Institute (JBI) 工具评估偏倚风险。使用 Stata®18 对纳入的具有可比结果的研究进行随机效应模型 meta 分析:结论:与健康儿童相比,儿童营养服务在定性和定量方面都显示出更高的 DDEs 患病率。元分析表明,在儿童慢性疾病患者中,出现定性缺陷的几率高于定量缺陷。由于数据不足,无法就治疗类型、开始治疗的年龄与 DDEs 患病率之间的关联得出结论。缺乏检测釉质缺陷的标准化方法给定性和定量分析带来了挑战。
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引用次数: 0
Interdisciplinary management of orofacial lesions in early childhood: paediatric alert system at University of Verona. 幼儿口面部损伤的跨学科管理:维罗纳大学的儿科警报系统。
IF 3.6 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-03 Epub Date: 2024-05-01 DOI: 10.23804/ejpd.2024.2141
N Zerman, A Zangani, C Maffeis, A Pietrobelli, G Piacentini, F Zotti, R De Manzoni, R Silva, A Cordiano, E Spinas, P F Nocini

Background: Orofacial trauma (OFT) occurs frequently in children and requires thorough evaluation not only by paediatric dentists but also by all specialists involved in emergency services, particularly in cases involving children under 3 years of age, given their inability to directly participate in clinical-anamnestic evaluations. Addressing early childhood orofacial trauma resulting from maltreatment, this study explores the key role played by various healthcare professionals, including paediatric dentists, general dentists, maxillofacial surgeons, dental hygienists, and paediatricians, in the optimal management of these cases. In the event of trauma due to suspected or confirmed mistreatment, it is essential that all healthcare workers involved have precise knowledge of the appropriate course of action from both a clinical and legal point of view, guaranteeing maximum protection for the young patient. This is particularly significant as cases of mistreatment with apparently minor consequences can degenerate into situations of irreparable severity. The latest guidelines from the International Association of Dental Traumatology (IADT) in 2020 continue to emphasise the potential correlation between OFT and cases of abuse or violence. Recent recommendations in the literature highlight the importance of facilitating mandatory reporting of incidents to relevant authorities and improving information sharing between dental healthcare professionals and child welfare services. A new flow diagram, called Paediatric Orofacial Trauma Alert (P.O.T.A.), has been proposed at the University of Verona. This tool is specifically designed to assist specialists dealing with early childhood orofacial trauma cases by assisting them in identifying potential cases of maltreatment. In this innovative approach, the collaborative efforts of general dentists, paediatric dentists, maxillofacial surgeons, dental hygienists and paediatricians play a vital role in cases of abuse. In addition to restoring the oral health of young patients, these professionals can activate a vast network of contacts, ensuring not only optimal oral health care but also providing comprehensive support to victims. The objective is to safeguard not only the physical but also the psychological well-being of these vulnerable subjects.

背景:颌面外伤(OFT)经常发生在儿童身上,不仅需要儿科牙医进行全面评估,还需要所有参与急救服务的专家进行全面评估,尤其是涉及 3 岁以下儿童的病例,因为他们无法直接参与临床-肛门内科评估。针对虐待导致的幼儿口面部创伤,本研究探讨了包括儿童牙医、普通牙医、颌面外科医生、牙科卫生员和儿科医生在内的各种医疗专业人员在这些病例的最佳治疗过程中发挥的关键作用。在发生疑似或已证实的虐待造成的创伤时,所有相关医护人员都必须准确掌握从临床和法律角度出发的适当行动方案,以确保为年幼患者提供最大程度的保护。这一点尤为重要,因为看似后果轻微的虐待案件可能会恶化成无法弥补的严重后果。国际牙科创伤学会(IADT)在 2020 年发布的最新指南中继续强调了口腔外伤与虐待或暴力之间的潜在关联。最近的文献建议强调了促进向相关机构强制报告事件以及改善牙科医疗保健专业人员与儿童福利服务机构之间信息共享的重要性。维罗纳大学(University of Verona)提出了一种名为 "儿童口面部创伤警报"(P.O.T.A.)的新流程图。该工具专门用于协助专家处理幼儿口面部创伤病例,帮助他们识别潜在的虐待病例。在这一创新方法中,普通牙医、儿童牙医、颌面外科医生、牙科卫生员和儿科医生的共同努力在虐待病例中发挥了至关重要的作用。除了恢复年轻患者的口腔健康外,这些专业人员还可以启动一个庞大的联系网络,不仅确保最佳的口腔保健,还为受害者提供全面的支持。这样做的目的不仅是为了保护这些弱势群体的身体健康,也是为了保护他们的心理健康。
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引用次数: 0
Dog-Assisted Therapy (DAT) for the management of anxiety during paediatric dental care. A scoping review. 狗辅助治疗(DAT)用于缓解儿科牙科护理过程中的焦虑。范围综述。
IF 2.2 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-03 Epub Date: 2024-03-01 DOI: 10.23804/ejpd.2024.2001
A Garrocho-Rangel, M Rosales-Bérber, D Ríos-Méndez, J Flores-Velázquez, D Trejo-Herbert, F Pozos-Guillén, A Pozos-Guillén

Aim: Distraction techniques in paediatric dentistry can be effective in decreasing the child's attention span from an unpleasant or stressful procedure. Distraction is achieved through imagination, audio, and/or visual stimuli. It has been shown that the accompaniment and participation of animals or pets, specifically dogs (Dog-Assisted Therapy or DAT), during medical, oral, and therapeutic activities can improve the physical and mental health of patients, especially children. However, there is limited information available regarding the impact of incorporating a certified therapy dog into the paediatric dental environment as a distraction strategy to alleviate anxiety levels during dental procedures.

Methods: This scoping review aimed to identify and review published articles concerning the use of DAT in paediatric dentistry. The article discusses indications, benefits, and potential risks to human health and safety in clinical settings. Eligible sources encompass clinical trials, observational studies, and narrative reviews written in either English or Spanish and published over the last two decades, sourced from four electronic databases. Ultimately, seven pertinent studies were included in the review.

Conclusion: DAT presents itself as a promising alternative in managing anxiety and stress among children during dental visits. The integration of a therapy dog and its handler into the paediatric oral care team should be thoughtfully considered by clinicians as a means to enhance the comfort and compliance of apprehensive patients.

目的:儿童牙科中的分散注意力技术可以有效地减少儿童对不愉快或紧张程序的注意力。分散注意力可以通过想象、声音和/或视觉刺激来实现。有研究表明,在医疗、口腔和治疗活动中,动物或宠物(特别是狗(狗辅助治疗或 DAT))的陪伴和参与可以改善患者(尤其是儿童)的身心健康。然而,关于在儿科牙科治疗环境中使用经认证的治疗犬作为分散注意力的策略以减轻牙科治疗过程中的焦虑水平所产生的影响,目前可获得的信息还很有限:本范围综述旨在确定和综述已发表的有关在儿童牙科中使用 DAT 的文章。文章讨论了在临床环境中使用 DAT 的适应症、益处以及对人体健康和安全的潜在风险。符合条件的资料来源包括用英语或西班牙语撰写的临床试验、观察性研究和叙述性综述,这些资料都是在过去二十年中发表的,来源于四个电子数据库。最终,七项相关研究被纳入综述:结论:DAT 是治疗儿童看牙期间的焦虑和压力的一种很有前景的替代方法。临床医生应深思熟虑地考虑将治疗犬及其训导员纳入儿科口腔护理团队,以此提高焦虑患者的舒适度和依从性。
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引用次数: 0
Different effects of whitening treatment on natural teeth and teeth treated with Icon resin: an experimental study in vitro. 美白处理对天然牙齿和用 Icon 树脂处理过的牙齿的不同影响:体外实验研究。
IF 3.6 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-03 Epub Date: 2024-03-01 DOI: 10.23804/ejpd.2024.2014
R Favero, M Pellarin, D Bresolato, A Di Fiore, A Zuccon, A Volpato, L Favero

Background: It is quite common for white spots to develop on a tooth, due sometimes to a defective formation of the enamel layer, and sometimes to patches of demineralisation as a result of poor oral hygiene during orthodontic treatment with fixed braces. ICON DMG is currently the only noninvasive treatment for white spots. After a preliminary etching, it infiltrates the enamel, filling the spaces between the prisms with a resinous material that has a refraction coefficient very similar to that of healthy tooth enamel. The aim of this study was to test the efficacy of professional whitening procedures on teeth previously treated with ICON. The study hypothesis was that infiltration with ICON resin creates a barrier capable of preventing the bleaching action of the whitening agent.

Materials: White spots were artificially created on one half of the vestibular surface of 12 human teeth, while the other half was protected with a composite adhesive. The white spots were infiltrated with ICON and the protective adhesive was subsequently removed. A professional teeth whitening procedure was then completed on both halves of the teeth. A statistical analysis was performed to compare spectrophotometric recordings obtained before and after the ICON infiltration and teeth whitening procedures.

Conclusion: The whitening procedure modified the colour of the teeth on the half not infiltrated with ICON (p<0.05), but there was no statistically significant change in colour on the half infiltrated with ICON. The presence of the ICON resin seems to act as a partial barrier to the action of the whitening agent.

背景:牙齿上出现白斑是很常见的现象,有时是由于釉质层的形成缺陷,有时是由于固定矫治器正畸治疗期间口腔卫生不良导致的脱矿斑。ICON DMG 是目前治疗白斑的唯一非侵入性疗法。在初步蚀刻后,它渗入珐琅质,用一种树脂材料填充棱镜之间的空隙,这种材料的折射系数与健康牙齿珐琅质的折射系数非常相似。这项研究的目的是测试专业美白程序对曾用 ICON 治疗过的牙齿的功效。研究假设是,ICON 树脂的浸润会形成一层屏障,能够阻止美白剂的漂白作用:材料:在 12 颗人类牙齿前庭表面的一半人为制造白斑,另一半用复合粘合剂保护。用 ICON 对白斑进行浸润,然后去除保护粘合剂。然后在两半牙齿上完成专业牙齿美白程序。对 ICON 渗入和牙齿美白程序前后的分光光度记录进行了统计分析比较:结论:美白程序改变了未浸润 ICON 的那一半牙齿的颜色(p
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引用次数: 0
Humanisation and health needs in children: present or future? 人性化与儿童的健康需求:现在还是未来?
IF 3.6 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-03 Epub Date: 2024-02-01 DOI: 10.23804/ejpd.2024.2012
A V Brescia, A Mastroianni, G Parisi, G Di Gennaro, R Docimo, L Montesani

Aim: For a few years, teledentistry has been an emerging innovative strategy with potential in the field of paediatric dentistry. There are still few studies in this regard, so further research is needed to verify and ensure that teledentistry is not only an accessible mode of communication, but above all effective and evidence-based. This study aimed to use a preliminary telematic approach to promote the compliance of patients in the developmental age during the first dental visit.

Materials: Two hundred patients were selected according to the eligibility criteria, and distributed in two groups: a study group with the preliminary telematic approach (ATP) before the first visit and a control group with traditional first visit without ATP. Through an ordinal semi-proportional regression model, the degrees of collaboration between the study and control groups were compared, correcting the estimate for age groups, the presence of systemic pathologies, disorders of cognition, attention and learning, degree of anxiety and previous medical-dental experiences.

Conclusion: The preliminary telematic approach could be useful as a support to the traditional paediatric dental visit, to promote better management and fidelity of the patient, reducing anxiety and increasing collaboration during the first visit.

目的:几年来,远程牙科治疗一直是儿童牙科领域一种新兴的、具有潜力的创新策略。但这方面的研究仍然很少,因此需要进一步的研究来验证并确保远程牙科治疗不仅是一种方便的交流方式,而且是有效的、有实证依据的。本研究旨在使用一种初步的远程医疗方法来促进发育期患者在首次牙科就诊时的依从性:材料:根据资格标准选取了 200 名患者,并将其分为两组:在首次就诊前使用初步远程信息处理方法(ATP)的研究组和不使用 ATP 的传统首次就诊对照组。通过一个序数半比例回归模型,比较了研究组和对照组之间的合作程度,并根据年龄组、是否存在系统性疾病、认知、注意力和学习障碍、焦虑程度以及以往的医疗牙科经验对估计值进行了修正:初步的远程医疗方法可作为传统儿童牙科就诊的辅助手段,促进更好地管理和忠实于患者,减少焦虑,加强首次就诊期间的合作。
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引用次数: 0
Silver diamine fluoride: A stem cell cytotoxicity and dentin growth factor release in-vitro study. 二胺氟化银:一项干细胞细胞毒性和牙本质生长因子释放的体外研究。
IF 3.6 2区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-03 Epub Date: 2023-06-01 DOI: 10.23804/ejpd.2023.1928
M M Khalil, A Bashir, Y Monjazebi, M Shah, A R Tawfik, I Hussein, M Jamal

Aim: To investigate the effect of 38% SDF and its serial dilutions on the Stem cells from Human Exfoliated Deciduous teeth (SHED) and its ability to release growth factors from deciduous dentine.

Methods: The viability of SHED post-exposure to 38%, 3.8%, 0.38%, 0.038%, and 0.0038% SDF were assessed at 2, 5, and 7 days using the CyQuant assay, and results were validated using the MTT assay. The osteogenic differentiation of the cells was also investigated post-exposure to 0.0038% SDF. The release of the growth factors; TGF-β1, FGF-b, BMP-2, and VEGF from deciduous dentin discs exposed to 38% SDF, 0.0038% SDF, Ca(OH)2, MTA, and 17% EDTA were examined using ELISA. Statistical analysis was performed using means and standard deviations (p < 0.05). Two-way ANOVA compared the means of more than two groups with Tukey's multiple comparison test. The unpaired t-test was also used to compare the differences between the two data sets.

Conclusion: 38% SDF released dentinogenic growth factors from dentin discs, potentially explaining its role in reactionary dentinogenesis. Moreover, 0.0038% SDF resulted in a non-cytotoxic concentration that enhanced cellular proliferation and released bioactive molecules from dentin comparable to the 38% concentration. After further investigations, the 0.0038% dilution of SDF could present itself as a clinical concentration.

目的:研究 38% SDF 及其系列稀释液对人类脱落牙齿(SHED)干细胞的影响及其从脱落牙本质中释放生长因子的能力:方法:使用 CyQuant 检测法评估暴露于 38%、3.8%、0.38%、0.038% 和 0.0038% SDF 后的 SHED 在 2、5 和 7 天的存活率,并使用 MTT 检测法验证结果。在暴露于 0.0038% SDF 后,还对细胞的成骨分化进行了研究。使用 ELISA 检测了暴露于 38% SDF、0.0038% SDF、Ca(OH)2、MTA 和 17% EDTA 的脱落牙本质盘中的生长因子 TGF-β1、FGF-b、BMP-2 和 VEGF 的释放情况。统计分析采用均值和标准差(P < 0.05)。用 Tukey's 多重比较检验对两组以上的均值进行双向方差分析。结论:38% 的 SDF 能从牙本质盘释放牙本质生成生长因子,这可能解释了它在反应性牙本质生成中的作用。此外,0.0038% 的 SDF 浓度无细胞毒性,可促进细胞增殖并从牙本质中释放生物活性分子,与 38% 的浓度相当。经过进一步研究,0.0038% 的 SDF 稀释液可作为一种临床浓度。
{"title":"Silver diamine fluoride: A stem cell cytotoxicity and dentin growth factor release in-vitro study.","authors":"M M Khalil, A Bashir, Y Monjazebi, M Shah, A R Tawfik, I Hussein, M Jamal","doi":"10.23804/ejpd.2023.1928","DOIUrl":"10.23804/ejpd.2023.1928","url":null,"abstract":"<p><strong>Aim: </strong>To investigate the effect of 38% SDF and its serial dilutions on the Stem cells from Human Exfoliated Deciduous teeth (SHED) and its ability to release growth factors from deciduous dentine.</p><p><strong>Methods: </strong>The viability of SHED post-exposure to 38%, 3.8%, 0.38%, 0.038%, and 0.0038% SDF were assessed at 2, 5, and 7 days using the CyQuant assay, and results were validated using the MTT assay. The osteogenic differentiation of the cells was also investigated post-exposure to 0.0038% SDF. The release of the growth factors; TGF-β1, FGF-b, BMP-2, and VEGF from deciduous dentin discs exposed to 38% SDF, 0.0038% SDF, Ca(OH)2, MTA, and 17% EDTA were examined using ELISA. Statistical analysis was performed using means and standard deviations (p < 0.05). Two-way ANOVA compared the means of more than two groups with Tukey's multiple comparison test. The unpaired t-test was also used to compare the differences between the two data sets.</p><p><strong>Conclusion: </strong>38% SDF released dentinogenic growth factors from dentin discs, potentially explaining its role in reactionary dentinogenesis. Moreover, 0.0038% SDF resulted in a non-cytotoxic concentration that enhanced cellular proliferation and released bioactive molecules from dentin comparable to the 38% concentration. After further investigations, the 0.0038% dilution of SDF could present itself as a clinical concentration.</p>","PeriodicalId":11930,"journal":{"name":"European journal of paediatric dentistry","volume":" ","pages":"108-112"},"PeriodicalIF":3.6,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10257146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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European journal of paediatric dentistry
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