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Effects of cross-linking agents on hydroxyproline release and root caries lesion size: Systematic review and network meta-analysis of in vitro studies. 交联剂对羟脯氨酸释放和龋齿根损大小的影响:体外研究的系统回顾和网络荟萃分析。
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-11-23 DOI: 10.1111/eos.13028
Isabela Monici Silva, Cecília de Brito Barbosa, Jéssica Alves de Cena, Erick Ribeiro, Fernanda Cristina Pimentel Garcia, Cristine Miron Stefani, Naile Dame-Teixeira

A promising approach for managing root caries is the use of cross-linking agents to stabilize collagen. However, despite testing various natural and synthetic agents in vitro, their efficacy remains uncertain. The aim of this review was to examine which cross-linking agent performs better in reducing root caries lesion depth and the release of hydroxyproline, which is a marker of collagen degradation. Studies evaluating the impact of cross-linking agents on dentin were included, while studies performed on enamel surface/cell cultures and studies evaluating collagenase inhibitors were excluded, among others. A comprehensive search covered eight databases, and study quality was assessed using the QUINN Tool for in vitro dental studies. Synthesis of the results was done using a Bayesian network meta-analysis to compare agents. Fifty studies involving 31 cross-linking agents were included for qualitative synthesis. The network meta-analysis for lesion depth involved 284 samples across 36 comparisons and ranked cross-linking agents in terms of their caries lesion depth-reducing effect (from best to worst): naringin > quercetin > riboflavin > proanthocyanidins > hesperidin > glutaraldehyde > cranberry > grape seed extract > untreated controls. Only naringin, quercetin, proanthocyanidins, and glutaraldehyde showed statistically significant efficacy over untreated controls. Cranberry extract excelled in reducing hydroxyproline release, followed by proanthocyanidins. In conclusion, proanthocyanidins positively affected both outcomes, suggesting they are prime candidates for translational research. Clinical studies are now essential to evaluate their real-world effectiveness against root caries. PROSPERO-CRD42023404911.

使用交联剂来稳定胶原蛋白是一种很有前景的治疗根龋的方法。然而,尽管对各种天然和合成制剂进行了体外测试,但其功效仍不确定。本综述旨在研究哪种交联剂在降低根龋病变深度和释放羟脯氨酸(胶原降解的标志)方面表现更好。其中包括评估交联剂对牙本质影响的研究,而不包括对釉质表面/细胞培养物进行的研究和评估胶原酶抑制剂的研究等。全面检索涵盖了八个数据库,并使用体外牙科研究 QUINN 工具对研究质量进行了评估。使用贝叶斯网络荟萃分析法对结果进行综合,以比较各种药物。定性综合纳入了涉及 31 种交联剂的 50 项研究。病变深度网络荟萃分析涉及 36 项比较中的 284 个样本,并根据交联剂降低龋损深度的效果(从最佳到最差)对其进行了排序:柚皮素 > 槲皮素 > 核黄素 > 原花青素 >橙皮甙 > 戊二醛 > 蔓越莓 > 葡萄籽提取物 > 未经处理的对照组。与未经处理的对照组相比,只有柚皮苷、槲皮素、原花青素和戊二醛具有显著的统计学功效。蔓越莓提取物在减少羟脯氨酸释放方面表现突出,其次是原花青素。总之,原花青素对两种结果都有积极影响,表明它们是转化研究的主要候选物质。现在必须开展临床研究,以评估它们对根部龋齿的实际效果。PROSPERO-CRD42023404911.
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引用次数: 0
Effect of masticatory muscle function on the craniofacial sutures of the anterior viscerocranium in growing rats. 咀嚼肌功能对生长期大鼠前粘膜颅面缝的影响
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-11-17 DOI: 10.1111/eos.13027
Hande Gorucu-Coskuner, Mustafa Al-Yassary, Kelly Billiaert, Stavros Kiliaridis, Gregory S Antonarakis

The transverse dimensions of internasal and nasopremaxillary sutures in rats were assessed at different ages and following different dietary functional demands.Eighty-four male Wistar rats were divided into two groups fed either a hard or a soft diet from 4 weeks of age, and sacrificed at 4 weeks (baseline: n = 12),16 weeks (n = 24), 26 weeks (n = 24), or 38 weeks (n = 24) of age. High-resolution micro-computed tomograms of the internasal and left nasopremaxillary sutures were obtained with 10 µm voxel size and 5 × 5 mm field of view. The endocranial and ectocranial suture widths, the mean suture width, and the suture height were measured. Linear regression analysis was used to estimate the effects of age and type of diet on suture dimensions. Internasal and nasopremaxillary suture dimensions were influenced by age, with a generally lower width and a larger height in the older animal. Regarding diet consistency, internasal suture widths were on average larger in the hard-diet group (e.g., mean suture width 43 µm in the hard- versus 30 µm in the soft-diet group at 26 weeks). In conclusion, the internasal and nasopremaxillary sutures tend to become narrower with age, while being influenced by masticatory functional demands.

将84只雄性Wistar大鼠分为两组,从4周龄开始喂食硬质或软质食物,分别在4周龄(基线:n = 12)、16周龄(n = 24)、26周龄(n = 24)或38周龄(n = 24)时处死。以 10 微米体素大小和 5 × 5 毫米视场获得了鼻内缝和左鼻颌下腺缝的高分辨率微型计算机断层扫描图。测量了颅内缝和颅外缝宽度、平均缝宽和缝高。线性回归分析用于估计年龄和饮食类型对缝合线尺寸的影响。鼻内缝和鼻上颌缝的尺寸受年龄影响,年龄较大的动物缝宽一般较低,缝高一般较高。在饮食浓度方面,硬质饮食组的鼻内缝宽度平均较大(例如,26周时,硬质饮食组的平均缝宽度为43微米,而软质饮食组的平均缝宽度为30微米)。总之,随着年龄的增长,鼻内缝和鼻上颌缝趋于变窄,同时受到咀嚼功能需求的影响。
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引用次数: 0
Gender variability in palpation performance for temporomandibular disorders with three different methods: An experimental study. 使用三种不同方法对颞下颌关节紊乱进行触诊时的性别差异:实验研究。
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-11-14 DOI: 10.1111/eos.13026
Hessamoddin Faghihian, Alicia Böthun, Birgitta Häggman-Henrikson, Maria Lalouni, Peter Svensson, Fredrik Hellström, Linus Andersson, Anna Lövgren

Gender differences in pain reporting and prevalence have been demonstrated. As gender of examiner also affects reporting of pain on palpation, the aim of this study was to evaluate the effects of examiner gender on palpation performance using three different palpation methods. Examiners of both genders were instructed on the correct technique for each palpation method by either a female or male instructor. The target was to achieve a pressure of 100 kPa, corresponding to the 1 kg of force for muscle palpation described in the diagnostic criteria for temporomandibular disorders (DC/TMD). All examiners performed traditional palpation with the index finger, palpation with a palpometer, and a novel bimanual palpation with visual feedback, in a randomized order. The examiner's gender, and its interaction with the gender of the instructor did not significantly affect magnitude, accuracy, or precision of the pressure applied. The method of palpation was the only factor that significantly impacted all metrics. The palpometer achieved the lowest palpation magnitude (mean pressure applied = 113.7  kPa, 95% CI: 109.8-117.6), the highest accuracy (absolute difference = 15.7  kPa, 95% CI: 12.3-19.1), and the highest precision (mean coefficient of variation = 6.8, 95% CI: 6.0-7.6), followed by bimanual palpation and traditional palpation. The results suggest that gender differences in pain reporting in patients are not likely to be a result of the technical aspects associated with the gender of the examiner. Instead, these differences may be attributed to other factors, such as sociocultural influences.

疼痛的报告和发生率存在性别差异。由于检查者的性别也会影响触诊时的疼痛报告,因此本研究旨在通过三种不同的触诊方法,评估检查者的性别对触诊表现的影响。在每种触诊方法中,由女性或男性指导员对男女考生进行正确技术指导。目标是达到 100 kPa 的压力,相当于颞下颌关节紊乱症(DC/TMD)诊断标准中规定的 1 kg 肌肉触诊力。所有检查者都按照随机顺序进行了传统的食指触诊、使用触诊计触诊和带有视觉反馈的新型双指法触诊。检查者的性别及其与指导者性别的交互作用对压力的大小、准确性和精确度没有显著影响。触诊方法是唯一对所有指标都有显著影响的因素。触诊器的触诊幅度最小(平均施压 = 113.7 kPa,95% CI:109.8-117.6),准确度最高(绝对差值 = 15.7 kPa,95% CI:12.3-19.1),精确度最高(平均变异系数 = 6.8,95% CI:6.0-7.6),其次是双指法触诊和传统触诊。结果表明,患者在疼痛报告方面的性别差异可能并不是与检查者性别相关的技术问题造成的。相反,这些差异可能归因于其他因素,如社会文化的影响。
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引用次数: 0
Bond strength of resin-based restorative materials to fast-setting calcium silicate cement using different resin adhesive systems. 使用不同树脂粘合剂系统的树脂基修复材料与速凝硅酸钙水泥的粘合强度。
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-27 DOI: 10.1111/eos.13025
Bahram Ranjkesh, Hilde M Kopperud, Henrik Løvschall

This study assessed the bond strength of resin-based restorative materials to fast-setting calcium silicate cement (Aarhus Uinversity, Denmark) when treated with each of two one-bottle universal adhesive systems. The cement surface (N = 256) was treated with a self-priming adhesive and a self-etch phosphate monomer-containing adhesive with and without etching of the cement surface. Specimens then received either resin composite or compomer restorative materials (n = 32). The bond strength was measured after 1 day and 1500 thermocycles (n = 16). The failure type was visually inspected. The cement-adhesive-restorative material interface was visualized using scanning electron microscopy (SEM). The data were analyzed using multiple linear regression. Restorative material type, resin adhesive system, and thermocycling had a statistically significant effect on the bond strength. Compomer restorative material and self-etch universal adhesive system demonstrated statistically significantly higher bond strength values to fast-setting calcium silicate cement, predominantly exhibiting cement cohesive failure. Etching the cement surface enhanced the bond strength of the self-priming universal adhesive. Thermocycling significantly reduced the bond strength. SEM showed self-etch universal adhesive seemingly diffused over the etched cement surface compared to other groups. Self-etch phosphate monomer-containing universal adhesive and compomer resulted in the highest bond strength to fast-setting calcium silicate cement.

本研究评估了树脂基修复材料与快速固化硅酸钙水泥(丹麦奥胡斯大学)在使用两种单瓶通用粘合剂系统处理时的粘结强度。使用自吸式粘合剂和含磷酸单体的自酸蚀粘合剂处理水泥表面(N = 256),并对水泥表面进行蚀刻和不蚀刻处理。然后,试样接受树脂复合材料或复合材料修复材料(n = 32)。在 1 天和 1500 个热循环后测量粘接强度(n = 16)。对失败类型进行目测。使用扫描电子显微镜(SEM)观察骨水泥-粘接剂-修复材料的界面。使用多元线性回归分析数据。修复材料类型、树脂粘接系统和热循环对粘接强度有显著的统计学影响。从统计学角度看,复合修复材料和自酸蚀通用粘合剂系统与速凝硅酸钙水门汀的粘结强度值明显更高,主要表现为水门汀粘结失效。蚀刻水门汀表面增强了自喷涂通用粘接剂的粘接强度。热循环明显降低了粘接强度。扫描电子显微镜显示,与其他组别相比,自喷通用粘合剂似乎扩散到了蚀刻水泥表面。含磷酸单体的自酸蚀通用粘合剂和复合材料与速凝硅酸钙水泥的粘合强度最高。
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引用次数: 0
Different adhesive approaches for the bonding of a new universal resin cement to a disilicate glass-ceramic. 将新型通用树脂粘接剂与二硅酸盐玻璃陶瓷粘接的不同粘接方法。
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-25 DOI: 10.1111/eos.13024
Anna Caroliny Detogni, Vitaliano Gomes Araújo-Neto, Renally Bezerra Wanderley Lima, Mário Alexandre Coelho Sinhoreti

This study aimed to evaluate the shear bond strength (SBS) when bonding a universal resin cement to a disilicate glass-ceramic using different adhesive protocols. Sixty specimens were etched with 10% hydrofluoric acid (HF) for 20 s and assigned to one of four treatment protocols (n = 15): The Positive control protocol comprised use of a universal adhesive system + adhesive resin cement; the Test1 protocol comprised use of a new universal adhesive system + universal resin cement; the Test2 protocol comprised use of a silane coupling agent + universal resin cement; and the use of a universal resin cement without adhesive and silane served as the Negative control. One of the two resin cement cylinders built on each specimen in each group was used to test for 24-h SBS, while the other was thermocycled and then tested for SBS. Data were submitted to two-way ANOVA, Tukey's test, and Weibull analysis. The negative control (24 h) showed the lowest SBS mean value. The Test2 protocol resulted in the highest SBS mean value after thermocycling. Adhesive and mixed failures were prevalent in all groups. No statistical difference in m values was observed among the groups at 24 h. After thermocycling, the two Test protocols showed the highest m values. The m values were significantly lower after thermocycling than at 24 h for all groups. After thermal aging, The Test2 protocol resulted in a statistically significantly higher SBS mean value after thermal aging than seen for the other groups.

本研究旨在评估使用不同粘接方案将通用树脂粘接剂粘接到二硅酸盐玻璃陶瓷时的剪切粘接强度(SBS)。60 个试样用 10%氢氟酸(HF)腐蚀 20 秒,然后分配到四种处理方案之一(n = 15):阳性对照方案包括使用通用粘合剂系统+粘合树脂水泥;测试 1 方案包括使用新型通用粘合剂系统+通用树脂水泥;测试 2 方案包括使用硅烷偶联剂+通用树脂水泥;使用不含粘合剂和硅烷的通用树脂水泥作为阴性对照。在每组试样上制作的两个树脂水泥圆柱体中,一个用于测试 24 小时 SBS,另一个进行热循环,然后测试 SBS。对数据进行了双向方差分析、Tukey 检验和 Weibull 分析。阴性对照(24 小时)的 SBS 平均值最低。热循环后,Test2 方案的 SBS 平均值最高。所有组中都普遍存在粘合和混合故障。热循环后,两个 Test 方案的 m 值最高。所有组的 m 值在热循环后都明显低于 24 小时时的值。热老化后,Test2 方案的 SBS 平均值在统计上明显高于其他组别。
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引用次数: 0
Psychological resilience in patients with primary Sjögren's syndrome: effect of involvement of major salivary and lacrimal glands. 原发性斯约格伦综合征患者的心理适应能力:主要唾液腺和泪腺受累的影响。
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-16 DOI: 10.1111/eos.13022
Esra Sevimli, Seda Günay, Alida Aliyeva, Burcu Aksoy, Farida Fortune, Nevsun Inanc, Gonca Mumcu

The aim of this study was to assess whether the involvement of major salivary and lacrimal glands in primary Sjögren's syndrome (pSS) affected the psychological resilience of patients. This cross-sectional study included 116 patients with pSS. Data were collected through clinical examinations, measurement of salivary flow rates (SFRs), and from Schirmer's test, as well as from patient-reported outcome measures (PROMs), such as the European League Against Rheumatism (EULAR) Sjögren's Syndrome Patient Reported Index (ESSPRI), Brief Resilience Scale (BRS), Work Productivity and Activity Impairment (WPAI), Oral Health Impact Profile-14 (OHIP-14), and the Hospital Anxiety and Depression Scale (HADS). Hyposalivation was defined as an unstimulated SFR of ≤0.1 mL/min. The BRS score (mean ± SD = 2.60 ± 0.69) was lower in patients with recurrent parotitis (2.11 ± 0.37) than in those without recurrent parotitis (2.67 ± 0.86), in the whole group. In patients with an unacceptable symptom state (ESSPRI score ≥ 5 points), a lower BRS score was observed in patients with both hyposalivation and ocular dryness (2.59 ± 0.69) than in patients with isolated hyposalivation (2.84 ± 0.84). The BRS score was also negatively associated with the WPAI-Daily Impairment and OHIP-14 scores in patients with hyposalivation as well as with HADS-A (the seven items of HADS relating to the anxiety dimension) and HADS-D (the seven items of HADS relating to the depression dimension) in the whole group. The results suggest that psychological resilience in pSS may be affected by recurrent parotitis, the levels of anxiety and depression, as well as hyposalivation with ocular dryness.

本研究旨在评估原发性斯约格伦综合征(pSS)的主要唾液腺和泪腺受累是否会影响患者的心理适应能力。这项横断面研究包括 116 名原发性斯约格伦综合征患者。研究人员通过临床检查、唾液流速(SFR)测量、施尔默试验以及患者报告结果测量(PROMs)收集数据,如欧洲抗风湿联盟(EULAR)的斯约格伦综合征患者报告指数(ESSPRI)、简易复原力量表(BRS)、工作效率和活动障碍(WPAI)、口腔健康影响档案-14(OHIP-14)以及医院焦虑抑郁量表(HADS)。唾液分泌过少定义为非刺激性 SFR ≤0.1 mL/min。整组患者中,复发性腮腺炎患者的 BRS 评分(平均值 ± SD = 2.60 ± 0.69)(2.11 ± 0.37)低于非复发性腮腺炎患者(2.67 ± 0.86)。在症状状态不可接受(ESSPRI 评分≥ 5 分)的患者中,同时存在唾液分泌过少和眼部干燥的患者(2.59 ± 0.69)的 BRS 评分低于单独存在唾液分泌过少的患者(2.84 ± 0.84)。BRS评分与唾液分泌过少患者的WPAI-日常障碍和OHIP-14评分呈负相关,与全组患者的HADS-A(HADS中与焦虑维度相关的7个项目)和HADS-D(HADS中与抑郁维度相关的7个项目)也呈负相关。研究结果表明,复发性腮腺炎、焦虑和抑郁程度以及唾液分泌过少和眼部干燥可能会影响 pSS 患者的心理适应能力。
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引用次数: 0
Effect of propolis added to single-bottle adhesives on water permeation through the hybrid layer. 单瓶粘合剂中添加蜂胶对混合层透水性的影响。
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-10 DOI: 10.1111/eos.13023
Lucineide Silva da Rocha, Daniela Ferreira de Oliveira, Cinthya Luna Veloso de Lima, Ticiano Gomes do Nascimento, Johnnatan Duarte de Freitas, Jeniffer Mclaine Duarte de Freitas, Isabel Cristina Celerino de Moraes Porto

Water treeing and water droplets are observed within adhesive layers and on the hybridized surface after bonding sound dentin using single-bottle etch-and-rinse adhesives, indicating permeability of the hybrid layer to water. The aim of this study was to assess the efficacy of dentin sealing by adhesives containing propolis by quantifying the area of water transudation from dentinal tubules after dentin hybridization. Brazilian red propolis was added to experimental adhesive and Single Bond (3M/ESPE) adhesive; experimental adhesive and Single Bond without propolis were used as controls. Under simulated pulp pressure, two layers of adhesive were applied to etched human dentin discs. Three minutes after light-curing, the hybridized dentin surface was replicated, and epoxy resin replicas were created to obtain scanning electron microscope images. Data were evaluated using ANOVA and Tukey's test. Single Bond containing propolis significantly decreased water permeation through the hybrid layer compared with the control group. Three minutes after polymerization, the experimental adhesive without propolis had formed a permeable hybrid layer. The addition of Brazilian red propolis significantly reduced surface water on hybridized dentin in a concentration-dependent manner. Two-step etch-and-rinse adhesives containing propolis were effective in reducing water permeation through the hybridized dentin surface.

使用单瓶蚀刻-冲洗粘合剂粘合健全牙本质后,在粘合剂层内和杂化表面可观察到水树化和水滴,这表明杂化层具有透水性。本研究的目的是通过量化牙本质杂交后牙本质小管的水渗出面积,评估含有蜂胶的粘合剂密封牙本质的功效。实验用粘合剂和 Single Bond(3M/ESPE)粘合剂中添加了巴西红蜂胶;实验用粘合剂和不含蜂胶的 Single Bond 用作对照组。在模拟牙髓压力下,将两层粘合剂涂在蚀刻的人类牙本质圆盘上。光固化三分钟后,复制杂化的牙本质表面,并制作环氧树脂复制品,以获得扫描电子显微镜图像。数据采用方差分析和 Tukey 检验进行评估。与对照组相比,含蜂胶的单一粘结剂可明显减少杂交层的透水性。聚合三分钟后,不含蜂胶的实验粘合剂已形成可渗透的混合层。添加巴西红蜂胶可显著减少杂化牙本质表面的水分,其减少程度与浓度有关。含有蜂胶的两步腐蚀-冲洗粘合剂可有效减少杂化牙本质表面的水分渗透。
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引用次数: 0
Three-dimensional finite element analysis of the impact of access cavity preparation on first molar fracture resistance: A scoping review. 三维有限元分析检修洞准备对第一磨牙抗折性的影响:范围综述。
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-09-30 DOI: 10.1111/eos.13021
Chuang Zhou, Ruochen Pu, Bin Liu

Access cavity preparation represents the initial step in root canal treatment. Minimally invasive approaches have gained increasing attention and involve advancements in the traditional access cavity preparation. Simultaneously, the development of three-dimensional finite element analysis (3D-FEA) has provided a theoretical foundation for evaluating the merits and drawbacks of various access cavity preparations. Studies using static loading 3D-FEA have suggested that conservative access cavity preparation reduces the concentration of stress in the cervical region, thereby strengthening fracture resistance. However, the lack of support from clinical data raises concerns about the validity of this suggestion. Conversely, studies involving cyclic loading 3D-FEA and dynamic loading 3D-FEA have challenged the prevailing perspectives by taking into account additional factors such as filling materials, thus providing a more comprehensive understanding of the impact of access cavity preparation on fracture resistance. Existing research lacks a comprehensive comparison of the different 3D-FEA methods, and this review fills this gap by providing a systematic assessment of different 3D-FEA methods and their applications in access cavity preparation.

入路腔预备是根管治疗的第一步。微创方法日益受到关注,并在传统的通路洞预备中取得了进步。与此同时,三维有限元分析(3D-FEA)的发展为评估各种通路洞预备方法的优缺点提供了理论基础。使用静态加载三维有限元分析的研究表明,保守的入路腔准备可减少颈椎区域的应力集中,从而增强抗骨折能力。然而,由于缺乏临床数据的支持,人们对这一观点的有效性产生了担忧。相反,涉及循环加载 3D-FEA 和动态加载 3D-FEA 的研究通过考虑填充材料等额外因素,对普遍观点提出了挑战,从而更全面地了解了入路腔准备对抗骨折性的影响。现有研究缺乏对不同 3D-FEA 方法的全面比较,本综述通过对不同 3D-FEA 方法及其在通路空腔制备中的应用进行系统评估,填补了这一空白。
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引用次数: 0
Effects of sodium copper- and sodium iron-chlorophyllin primers on resin bonding to dentin 铜钠和铁钠叶绿素底漆对牙本质树脂粘合的影响
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-09-24 DOI: 10.1111/eos.13017
Yohsuke Taira, Takafumi Egoshi, Kei Kaida, Shizuka Yamada

This study evaluated the effects of two chlorophyll derivatives, sodium copper chlorophyllin (Cu-Chl) and sodium iron chlorophyllin (Fe-Chl), on the bond strength between a self-curing luting agent (4-META/MMA-TBB resin) and dentin. Five aqueous primers containing 35% 2-hydroxyethylmethacrylate with 0.007% Cu-Chl, 0.07% Cu-Chl, 0.007% Fe-Chl, 0.07% Fe-Chl, or neither Cu-Chl nor Fe-Chl (no-Chl) were prepared. The extracted human dentin surfaces were etched with 10% phosphoric acid (10PA), primed, and bonded to a resin block using the 4-META/MMA-TBB resin. A conventional etching agent (10-3) and 10PA without primer (PA/no-primer) were used as controls. The microtensile bond strength was determined after 48 h. The arithmetic medians for 20 stick specimens were calculated and statistically analyzed using a nonparametric Steel-Dwass test (α = 0.05). The maximum bond strength was achieved in the 0.007% Cu-Chl group, followed by those in the 0.07% Cu-Chl, 0.07% Fe-Chl, 10-3, 0.007% Fe-Chl, no-Chl, and PA/no-primer groups. No significant difference was observed between 0.007% Fe-Chl, 0.07% Fe-Chl, and 10-3. The bond strength to dentin etched with 10PA was influenced by the type and concentration of the chlorophyll derivatives applied. Cu-Chl rather than Fe-Chl should be useful as a component of surface treatment agents for bonding 4-META/MMA-TBB resin to dentin.

本研究评估了两种叶绿素衍生物--叶绿素铜钠(Cu-Chl)和叶绿素铁钠(Fe-Chl)--对自固化粘接剂(4-META/MMA-TBB树脂)和牙本质之间粘接强度的影响。我们制备了五种水性底漆,分别含有 35% 的 2- 羟乙基甲基丙烯酸酯和 0.007% 的 Cu-Chl、0.07% 的 Cu-Chl、0.007% 的 Fe-Chl、0.07% 的 Fe-Chl,或者既没有 Cu-Chl,也没有 Fe-Chl(无 Chl)。用 10%磷酸(10PA)对拔出的人类牙本质表面进行蚀刻、打底,然后用 4-META/MMA-TBB 树脂将其粘结到树脂块上。常规蚀刻剂(10-3)和不含底剂的 10PA 作为对照。计算 20 个试样的算术中值,并使用非参数的 Steel-Dwass 检验(α = 0.05)进行统计分析。0.007% Cu-Chl 组的粘结强度最大,其次是 0.07% Cu-Chl、0.07% Fe-Chl、10-3、0.007% Fe-Chl、无 Chl 和 PA/无引物组。在 0.007% Fe-Chl、0.07% Fe-Chl 和 10-3 之间没有观察到明显的差异。用 10PA 蚀刻的牙本质的粘接强度受叶绿素衍生物的类型和浓度的影响。在将 4-META/MMA-TBB 树脂与牙本质粘合时,Cu-Chl 而不是 Fe-Chl 应作为表面处理剂的一种成分。
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引用次数: 0
Effect of interleukin-17A on inflammatory mediator production in interleukin-1β-stimulated human dental pulp fibroblasts 白细胞介素-17A 对白细胞介素-1β 刺激人牙髓成纤维细胞产生炎症介质的影响
IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-09-20 DOI: 10.1111/eos.13019
Tadashi Nakanishi, Katsuhiro Mieda, Hitomi Kuramoto, Daisuke Takegawa

In response to pro-inflammatory cytokines such as interleukin (IL)-1β, dental pulp fibroblasts produce various inflammatory mediators, including IL-6, IL-8, CC chemokine ligand 20 (CCL20), and CXC chemokine ligand 10 (CXCL10), leading to the progression of pulpitis. IL-17/IL-17A (IL-17A) is a pro-inflammatory cytokine secreted by T helper (Th) 17 cells following their recruitment to inflamed sites; however, the roles of IL-17A during pulpitis remain unclear. The purpose of this study was to investigate the effect of IL-17A on IL-6, IL-8, CCL20 and CXCL10 production by human dental pulp fibroblasts (HDPFs) in vitro. IL-17A at a concentration of 100 ng/ml induced the production of 10 times more IL-8 and 4 times more CXCL10, but not IL-6 and CCL20, compared to controls. Co-stimulation of HDPFs with IL-17A and IL-1β synergistically enhanced the production of IL-6, CCL20, IL-8 and CXCL10. IL-1β increased expression of IL-17 receptor/IL-17RA (IL-17R) on HDPFs. Moreover, the cell signal pathways of p38 mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) were more potently activated by simultaneous stimulation with IL-17A and IL-1β. These findings suggest that IL-17A participates in the progression of dental pulp inflammation through the enhanced production of inflammatory mediators in HDPFs.

为应对白细胞介素(IL)-1β 等促炎细胞因子,牙髓成纤维细胞会产生各种炎症介质,包括 IL-6、IL-8、CC 趋化因子配体 20(CCL20)和 CXC 趋化因子配体 10(CXCL10),从而导致牙髓炎的恶化。IL-17/IL-17A(IL-17A)是T辅助(Th)17细胞被招募到发炎部位后分泌的一种促炎细胞因子;然而,IL-17A在牙髓炎中的作用仍不清楚。本研究旨在探讨 IL-17A 对体外人牙髓成纤维细胞(HDPFs)产生 IL-6、IL-8、CCL20 和 CXCL10 的影响。与对照组相比,浓度为 100 ng/ml 的 IL-17A 诱导的 IL-8 和 CXCL10 的产生量分别增加了 10 倍和 4 倍,而 IL-6 和 CCL20 的产生量则没有增加。用IL-17A和IL-1β共同刺激HDPF可协同增强IL-6、CCL20、IL-8和CXCL10的产生。IL-1β 增加了 HDPFs 上 IL-17 受体/IL-17RA(IL-17R)的表达。此外,p38 丝裂原活化蛋白激酶(MAPK)和核因子-κB(NF-κB)等细胞信号通路在 IL-17A 和 IL-1β 的同时刺激下被更有效地激活。这些研究结果表明,IL-17A 通过增强 HDPFs 中炎症介质的产生参与了牙髓炎症的进展。
{"title":"Effect of interleukin-17A on inflammatory mediator production in interleukin-1β-stimulated human dental pulp fibroblasts","authors":"Tadashi Nakanishi,&nbsp;Katsuhiro Mieda,&nbsp;Hitomi Kuramoto,&nbsp;Daisuke Takegawa","doi":"10.1111/eos.13019","DOIUrl":"10.1111/eos.13019","url":null,"abstract":"<p>In response to pro-inflammatory cytokines such as interleukin (IL)-1β, dental pulp fibroblasts produce various inflammatory mediators, including IL-6, IL-8, CC chemokine ligand 20 (CCL20), and CXC chemokine ligand 10 (CXCL10), leading to the progression of pulpitis. IL-17/IL-17A (IL-17A) is a pro-inflammatory cytokine secreted by T helper (Th) 17 cells following their recruitment to inflamed sites; however, the roles of IL-17A during pulpitis remain unclear. The purpose of this study was to investigate the effect of IL-17A on IL-6, IL-8, CCL20 and CXCL10 production by human dental pulp fibroblasts (HDPFs) in vitro. IL-17A at a concentration of 100 ng/ml induced the production of 10 times more IL-8 and 4 times more CXCL10, but not IL-6 and CCL20, compared to controls. Co-stimulation of HDPFs with IL-17A and IL-1β synergistically enhanced the production of IL-6, CCL20, IL-8 and CXCL10. IL-1β increased expression of IL-17 receptor/IL-17RA (IL-17R) on HDPFs. Moreover, the cell signal pathways of p38 mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) were more potently activated by simultaneous stimulation with IL-17A and IL-1β. These findings suggest that IL-17A participates in the progression of dental pulp inflammation through the enhanced production of inflammatory mediators in HDPFs.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"132 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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European Journal of Oral Sciences
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