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Survey of viruses infecting Solanaceous plants and characterization of Tomato brown rugose fruit virus (ToBRFV) infecting pepper in Egypt 埃及茄科植物病毒感染情况调查及感染辣椒的番茄褐皱纹果病毒(ToBRFV)特征分析
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.342111
Khalifa, M.A.A, A.M. El- Shazly, M.A.S. El- Kady, A.M. Al Naggar
: This study investigated the incidence and occurrence of virus-like symptoms in solanaceous plants, peppers (Capsicum spp.), potatoes (Solanum tuberosum) and tomatoes (Solanumlycopersicum) in Egypt. In this study, a total of 640 samples collected from different Governorates in Egypt. 640 samples were tested using DAS-ELISA, with 86.87% of samples showing positive reactions. It was also, revealed that Potato virus Y (PVY) was the most prevalent virus (16.41%), followed by Potatoleafrollvirus (PLRV) (11.87%), Alfalfamosaic virus (AMV) and Pepper mild mottle virus (PepMMoV) both (10.94%), However, Tomato spottedwiltvirus (TSWV) was detected at low frequency (4.22%). Some samples were found coinfected with two or three different viruses (i. e. PVY+ ToMV, TMV+ PepMMoV+ TYLCV). Results demonstrated that potato was the most infected plans (37.65%) followed by tomato (27.97%) and then by pepper (21.25%). Potato was the most infected plant (37.65%), followed by tomato (27.97%), and then pepper (21.25%). Collected pepper leaf and fruit with negative results in all tested antisera were selected for identification the virus isolates which suggestive to be Tomatobrownrugosefruitvirus (ToBRFV) on the basis of symptomatology, host range, modes of transmission and particle morphology. RT-PCR was used to confirm the identity of the virus isolate. Among twenty-five plants species and cultivars, the virus isolate reacted positively with fourteen plants species. The virus was transmitted mechanically and by seed 1:2.5%. Examination of leaf tissue extracts of infected pepper plants by electron microscope revealed rod–shaped particles of 261-348 nm in length, in the range of the Tobamovirus group. Concerning varietal susceptibility, Helsinki and Mycelia pepper cultivars were highly susceptible (70%) followed by California Wonder (50%) and Vita Z 461 (40%). On the other hand, Vita Z Dragon cultivar was the lowest susceptible one (30%). RT-PCR analysis indicated that the virus isolate is ToBRFV.
:本研究调查了埃及茄科植物辣椒(Capsicum spp.)、马铃薯(Solanum tuberosum)和西红柿(Solanumlycopersicum)中病毒样症状的发生率。在这项研究中,共收集了来自埃及不同省份的 640 个样本。使用 DAS-ELISA 对 640 个样本进行了检测,86.87% 的样本呈阳性反应。研究还发现,马铃薯病毒 Y(PVY)是最普遍的病毒(16.41%),其次是马铃薯卷叶病毒(PLRV)(11.87%)、马铃薯花叶病毒(AMV)和辣椒轻微斑驳病毒(PepMMoV)(均为 10.94%),但番茄斑萎病毒(TSWV)的检测频率较低(4.22%)。有些样品同时感染了两种或三种不同的病毒(即 PVY+ ToMV、TMV+ PepMMoV+ TYLCV)。结果表明,马铃薯是感染病毒最多的作物(37.65%),其次是番茄(27.97%)和辣椒(21.25%)。马铃薯是受感染最多的植株(37.65%),其次是番茄(27.97%),然后是辣椒(21.25%)。根据症状、寄主范围、传播方式和颗粒形态,采集的辣椒叶片和果实在所有抗血清检测中均为阴性,因此被选为病毒分离物进行鉴定,结果表明这些分离物为番茄褐纹果实病毒(ToBRFV)。RT-PCR 被用来确认病毒分离物的身份。在 25 个植物品种和栽培品种中,病毒分离物与 14 个植物品种呈阳性反应。病毒的机械传播和种子传播比例为 1:2.5%。用电子显微镜检查受感染辣椒植株的叶片组织提取物,发现长度为 261-348 nm 的杆状颗粒,属于托巴莫病毒组。关于品种的易感性,赫尔辛基和菌丝体辣椒品种的易感性较高(70%),其次是加利福尼亚奇迹(50%)和 Vita Z 461(40%)。另一方面,Vita Z Dragon 栽培品种的感病率最低(30%)。RT-PCR 分析表明,病毒分离物为 ToBRFV。
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引用次数: 0
Efficacy of two biocides on biological aspects of the spider, Chericanthium jovium Denis (Araneida: Eutichuridae) at Fayoum Governorate 两种杀菌剂对法尤姆省蜘蛛 Chericanthium jovium Denis(蛛形纲:Eutichuridae)生物学特性的影响
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.342632
El-Khouly, N. M.
: The present work were conducted to determine the effect of two biocides (i
:本研究旨在确定两种杀菌剂(i
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引用次数: 0
Rural residents benefit from social protection programs provided by NGOs in Fayoum Governorate 法尤姆省的农村居民受益于非政府组织提供的社会保护计划
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.342640
Safaa Ragaey Abdel Nabby El Saied
: This study aimed to: describe the level of respondents’ benefit from social protection programs )educational protection-health protection-economic protection-improving infrastructure( provided by civil society organizations in the study area, determine the relative importance of each social protection program from the perspective of the respondents, identify the variables. related to and affecting the degree to which respondents benefit from social protection programmes. To achieve these goals, a regular random sample of families benefiting from social protection programs was selected at a rate of )5%(, thus the sample size reached )236 rural families(. Frequency distribution tables, percentages, and arithmetic mean were used to display and describe the data. The alpha reliability coefficient )α( was also used to measure the degree of stability of the composite measures, using the Cronbach method. The simple correlation coefficient, the multiple linear regression coefficient, and step regression analysis were used. To identify the variables associated with and influencing rural residents’ benefit from social protection programs provided by civil society organizations. The most important results were
:本研究旨在:描述受访者从研究地区民间社会组织提供的社会保护计划(教育保护-健康保护-经济保护-改善基础设施)中受益的程度;从受访者的角度确定每个社会保护计划的相对重要性;确定与受访者从社会保护计划中受益的程度相关并对其产生影响的变量。为了实现这些目标,研究人员对受益于社会保护计划的家庭进行了定期随机抽样,抽样率为 5%,因此样本量达到 236 个农村家庭。研究使用了频率分布表、百分比和算术平均数来显示和描述数据。此外,还采用克朗巴赫法(Cronbach method)计算了阿尔法信度系数(alpha reliability coefficient )α(α),以衡量综合测量指标的稳定程度。使用了简单相关系数、多元线性回归系数和阶跃回归分析。确定与农村居民从民间组织提供的社会保护项目中受益相关并影响其受益的变量。最重要的结果是
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引用次数: 0
An economic study of the most important economic factors affecting the production of red meat in the New valley Governorate 对影响新山谷省红肉生产的最重要经济因素的经济研究
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.342624
Mervat Abo Elyazed Soliman Ashour
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引用次数: 0
Exogenously-applied plant growth biostimulators increase the growth and productivity of Vicia faba plants 外源施用的植物生长生物刺激剂可提高紫花苜蓿的生长和产量
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.344598
Mona A.A. Abdel-Kawy, Gamal F. Mohamed, Hanan A.A. Taie, Mostafa M. Rady
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引用次数: 0
Potential impacts of amino acids, putrescine and glycine betaine on productivity and fruit attributes of “Le-Conte” pear trees grown under water scarcity stress 氨基酸、腐胺和甘氨酸甜菜碱对缺水胁迫下生长的 "Le-Conte "梨树的产量和果实属性的潜在影响
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.342079
Mohamed A. Abd El-wahab, Naglaa H. Shakweer
: The problem of water shortage worries many countries in the world because its negative impact on many economic crops. In this study, the foliar application by amino acids 3 cm/l, putrescine 1.03 g/l , glycine betaine 1.35 g/l, betaine 0.45 g/l and proline 1.28 g/l. added to reduce stress These compounds were sprayed twice, the first spraying one month after fruit set, the second spraying two months after fruit set on "Le-Conte" pear trees that grow under conditions of water scarcity stress (60% of water requirements) and control (60% of water requirements) with any these compounds treatments. Water scarcity stress caused a reduction in vegetative parameters, macro nutrients, soluble proteins, lowest productivity per tree and fruit weight, fruit valium, fruit length and fruit width, TSS, and fruit attribute. However, the contents of proline, full phenol, and antioxidant enzyme activity were augmented in pear trees grown under water scarcity stress. application of amino acids, putrescine, and glycine betaine on stressed trees showed different responses and improvements in the yield, fruit attribute, morphological traits, macronutrients, reduction of oxidative stress and antioxidant enzyme activity. a Foliar spray amino acids 3 cm/L on "Le-Conte" pear trees grow under conditions of water scarcity stress (60% CWR) during after fruit set stage to harvest gave the greatest yield per tree, fruit weight, fruit valium, fruit length, fruit diameter, TSS and vegetative growing compared to control (60% CWR) in two seasons. It could be recommended that foliar spray of amino acids, putrescine and glycine betaine, especially amino acids, could be commercially intended for the stimulation of pear trees cultivated under water scarcity stress
:世界上许多国家都面临着缺水问题,因为它对许多经济作物产生了负面影响。本研究在 "Le-Conte "梨树上叶面喷施氨基酸 3 厘米/升、腐胺 1.03 克/升、甘氨酸甜菜碱 1.35 克/升、甜菜碱 0.45 克/升和脯氨酸 1.28 克/升,以减少压力。这些化合物喷洒了两次,第一次在坐果后一个月喷洒,第二次在坐果后两个月喷洒。缺水胁迫导致植被参数、宏观养分、可溶性蛋白质、最低单株产量、果实重量、果实容重、果实长度和宽度、总悬浮固体含量和果实属性降低。然而,在缺水胁迫下生长的梨树的脯氨酸、全酚含量和抗氧化酶活性都有所提高。在受胁迫的梨树上施用氨基酸、腐胺和甘氨酸甜菜碱后,在产量、果实属性、形态特征、宏养分、氧化胁迫降低和抗氧化酶活性方面都有不同的反应和改善。a 在 "Le-Conte "梨树上叶面喷施 3 cm/L 氨基酸,在坐果后至采收期的缺水胁迫条件下(60% CWR),与对照组(60% CWR)相比,两季的单株产量、果实重量、果实容重、果实长度、果实直径、总悬浮固体含量和无性生长量均最高。建议叶面喷施氨基酸、腐胺和甘氨酸甜菜碱,特别是氨基酸,可用于缺水胁迫下梨树的生长刺激。
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引用次数: 0
Antimicrobial activity of two natural plants grown in Egyptian environment: Ipomoea carnea and Tamarix nilotica 生长在埃及环境中的两种天然植物的抗菌活性:红苕和柽柳的抗菌活性
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.342083
Tarek Kamal El-Sawah, Rabea Mohamed El-Shahawy, Ashraf Ibrahim Nageeb, Khaled Mohamed Atalla
: Plants are the largest biochemical and pharmaceutical stores ever known on our planet. Some medicinal plants have not found wider application and sometimes are referred as "forgotten plants". Antimicrobial activity of Ipomoeacarnea and Tamarixnilotica were evaluated by disk diffusion method and broth microdilution method. Extraction was performed by ethanol 50%. Results showed antibacterial activity of Tamarixniloticaextract with inhibition zones; 15, 14 and 19 mm against Bacilluscereus, Listeriamonocytogenesand Staphylococcusaureus, respectively. Ipomoeacarneaextract revealed a good effect against Staph.aureuswith 18 mm inhibition zone and MIC value against Staph.aureus was 8 mg/ml. Gram positive bacteria; B.cereus and L. monocytogenesshowed MIC value at 16 mg/ml likewise E.coliwas at 16 mg/ml. Pseudomonas aeruginosa and Salmonellatyphimurium were inhibited at 64 and 32 mg/ml, respectively. Gram positive strains were also susceptible to Tamarixnilotica extract with MIC values from 8 to 32 mg/ml. Gram negative strains were more resistant as MIC value were at 64 and 128 mg/ml. Bactericidal activity (MLC) at 16 mg/ml for both extracts against Staph. aureus while P. aeruginosa showed MIC at 128 mg/ml with Tamarixnilotica extract. Fungal strains were more resistant to the tested plant extracts whereas Ipomoeacarneaextract showed MIC value 16 mg/ml against Penicilliumitalicumwhile Aspergillusflavusand Rhizopusstoloniferanot inhibited at the highest concentration 128 mg/ml the yeast strains: Saccharomycescerevisiaeand Candidaalbicans were inhibited at 64 mg/ml. Tamarixniloticaextract showed MIC values ranged from 64 to 128 mg/ml against all tested fungal strains. It is reasonable to assessing their applicability and benefits using modern scientific analysis methods.
:植物是地球上最大的生化和药物宝库。有些药用植物没有得到更广泛的应用,有时被称为 "被遗忘的植物"。通过磁盘扩散法和肉汤微量稀释法评估了 Ipomoeacarnea 和 Tamarixnilotica 的抗菌活性。提取采用 50%乙醇。结果表明,柽柳提取物具有抗菌活性,对枯草杆菌、李斯特菌和金黄色葡萄球菌的抑制区分别为 15、14 和 19 毫米。Ipomoeacarnea 提取物对金黄色葡萄球菌有很好的抑制作用,抑制区为 18 毫米,对金黄色葡萄球菌的 MIC 值为 8 毫克/毫升。革兰氏阳性菌;蜡样芽孢杆菌和单核细胞原生动物的 MIC 值为 16 毫克/毫升,同样,大肠杆菌的 MIC 值为 16 毫克/毫升。铜绿假单胞菌和沙门氏菌的抑菌浓度分别为 64 毫克/毫升和 32 毫克/毫升。革兰氏阳性菌也对柽柳提取物敏感,其 MIC 值为 8 至 32 毫克/毫升。革兰氏阴性菌株的耐药性较强,其 MIC 值分别为 64 毫克/毫升和 128 毫克/毫升。两种提取物对金黄色葡萄球菌的杀菌活性(MLC)均为 16 毫克/毫升,而铜绿假单胞菌对柽柳提取物的 MIC 值为 128 毫克/毫升。真菌菌株对所测试的植物提取物有更强的抵抗力,而 Ipomoeacarneae 提取物对青霉的 MIC 值为 16 毫克/毫升,而黄曲霉和根瘤菌在最高浓度 128 毫克/毫升时对酵母菌株没有抑制作用:酵母菌和白色念珠菌在 64 毫克/毫升的浓度下受到抑制。柽柳提取物对所有受试真菌菌株的 MIC 值介于 64 至 128 毫克/毫升之间。利用现代科学分析方法评估其适用性和益处是合理的。
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引用次数: 0
Social protection programs and their relationship to the quality of life of rural families in one of the villages of the Delingat District - Beheira Governorate 社会保护计划及其与贝希拉省德林加特区一个村庄农村家庭生活质量的关系
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.342615
Suzan M. Nasrat, Eman A.M. Khalifa
: This research aimed to determine the level of economic and social quality of life among the respondents in the study-village after they benefited from social protection programs, and to identify the most important characteristics of social protection programs, and the relationship of the studied independent variables to the overall level of quality of life of the respondents after they benefited from social protection programs. The research was conducted on a sample of 120 respondents from heads of households of both genders in the village of Al-Messin, Al-Delngat District in Al-Beheira Governorate.. The data was collected using a questionnaire form, and after collecting the data, it was transcribed and statistically analyzed using numerical count tables, percentages, Pearson's simple correlation coefficient, and the Chi-square test. The most important results were as follows:
:本研究旨在确定研究村受访者受益于社会保护计划后的经济和社会生活质量水平,并确定社会保护计划的最重要特征,以及所研究的自变量与受访者受益于社会保护计划后的整体生活质量水平之间的关系。研究以贝海拉省 Al-Delngat 区 Al-Messin 村的男女户主为样本,共 120 名受访者。数据收集采用问卷调查的形式,数据收集完毕后,对数据进行誊写,并采用数字统计表、百分比、皮尔逊简单相关系数和卡方检验进行统计分析。最重要的结果如下
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引用次数: 0
social support for rural women in Fayoum Governorate 法尤姆省农村妇女的社会支持
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.344596
Marwa Ahmed Galal Ewies
: The research aimed to build a quantitative measure of social support for rural women, determine the degree to which the subjects received social support from their husbands, family, friends and neighbors, and determine the significance of the relationship between the independent variables studied and the degree to which the subjects obtained social support, and the percentage of contribution of the independent variables studied in explaining the variance between the subjects. The most important problems you face and suggestions for solving them. The research was conducted in four villages affiliated with the Fayoum and Atsa centers, with a sample of 385 women from the villages selected for the study. The data was collected using a questionnaire form prepared for this purpose, through a personal interview with the respondents, during the months of January and February of 2023, after collecting the data, transcribing it, and analyzing it. Statistically, using numerical and percentage tables, simple correlation coefficient, chi-square, partial regression coefficient, and Spearman, Gutman, and Cronbach tests for scale stability, the most important results were as follows:
:本研究旨在建立农村妇女社会支持的定量衡量标准,确定研究对象从丈夫、家人、朋友和邻居那里获得社会支持的程度,并确定所研究的自变量与研究对象获得社会支持的程度之间关系的显著性,以及所研究的自变量在解释研究对象之间差异方面的贡献百分比。您面临的最重要问题及解决建议。研究在法尤姆和阿萨中心下属的四个村庄进行,从这些村庄中选取了 385 名妇女作为研究样本。在收集数据、誊写数据和分析数据后,于 2023 年 1 月和 2 月通过与受访者的个人访谈,使用为此目的准备的调查问卷收集数据。统计方面,使用了数字和百分比表、简单相关系数、卡方、部分回归系数以及斯皮尔曼、古特曼和克朗巴赫量表稳定性检验,最重要的结果如下:
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引用次数: 0
Antimicrobial activity of olive leaves extracts and application of leaves powder in meat preservation 橄榄叶提取物的抗菌活性和叶粉在肉类保鲜中的应用
Pub Date : 2024-01-01 DOI: 10.21608/fjard.2024.342104
Tarek Kamal El-Sawah, Rabea Mohamed El-Shahawy, Ashraf Ibrahim Nageeb, Khaled Mohamed Atalla
: The applications of natural antimicrobial compounds from plants in food becomes very frequent. Olive leaves extracts were assessed as antimicrobial agents. Petroleum ether, ethyl acetate, methanol, and ethanol 50% were used for extraction of phytochemicals and disk diffusion method was applied against tested bacterial strains. Ethanol 50% revealed the greater extraction yield 22.5% followed by methanol 8.2%. Ethyl acetate, methanol, and ethanol 50% extracts showed good antibacterial activity against Gram-positive strains at concentration 10 mg/disk whereas Listeria monocytogenes was the most susceptible strain followed by Staphylococcusaureus. In agreement with the current trend of raising the value to natural resources, the direct addition of natural compounds to food is the most common method of application. Olive leaves powder was added to minced meat as a preservative at concentrations of crude extract percent of ethanol 50% equivalent to dried olive leaves powder: 16 and 32 mg crude extract per gram of meat. Results indicated that minced meat treated with olive leaves powder showed a decrease in total viable count as well as total coliform count in comparison with control along storage period at 4 : 6°C. Staphylococcus aureus and Escherichiacoli were killed by the effect of olive leaves powder addition in meat samples. Organoleptic evaluation showed that olive leaves powder at concentration 16 mg/g enhanced the taste of minced meat with no significant differences in comparison with control sample in other sensory attributes.
:从植物中提取的天然抗菌化合物在食品中的应用越来越频繁。橄榄叶提取物被评估为抗菌剂。提取植物化学物质时使用了石油醚、乙酸乙酯、甲醇和 50%乙醇,并对测试的细菌菌株采用了盘扩散法。乙醇 50% 的提取率为 22.5%,甲醇为 8.2%。乙酸乙酯、甲醇和 50%乙醇提取物在 10 毫克/盘的浓度下对革兰氏阳性菌株具有良好的抗菌活性,而单核细胞增生李斯特菌是最易感菌株,其次是金黄色葡萄球菌。与当前提高自然资源价值的趋势一致,在食品中直接添加天然化合物是最常见的应用方法。将橄榄叶粉添加到碎肉中作为防腐剂,其粗提取物的浓度为乙醇的 50%,相当于干橄榄叶粉:每克肉含 16 和 32 毫克粗提取物。结果表明,与对照组相比,用橄榄叶粉处理过的碎肉在 4 : 6°C 的贮藏期间,总存活数和总大肠菌群数都有所减少。肉样中添加橄榄叶粉后,金黄色葡萄球菌和大肠杆菌被杀死。感官评估显示,浓度为 16 毫克/克的橄榄叶粉可改善肉糜的口感,但在其他感官属性方面与对照样本相比没有显著差异。
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引用次数: 0
期刊
Fayoum Journal of Agricultural Research and Development
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