Pub Date : 2019-11-15DOI: 10.25040/ecpb2019.03.005
M. Gzhegotskyi, N. Sukhodolska
In modern realities of adverse effects of ecosystem on human health, WHO experts have proposed a program of research “Human reproduction”, whose main task is to study the negative impact of environmental factors on the generative function [1-3]. Under the high technogenic loading conditions, the problem of harmful influence of heavy metals in combination with the imbalance of trace elements on woman’s organisms, which lead to a decrease in adaptation reserves with the further development of changes in most systems of the organism, is urgent [4-6]. Recent studies have already identified a correlation between the elevated levels of lead in the blood and the development of threatened abortion and premature birth syndrome [7,8]; excess of lead causes the miscarriage, intrauterine growth restriction, chronic fetal hypoxia, birth defects in newborn babies [9,10]. High levels of cadmium in the placenta adversely affect the anthropometric data of the newborn – weight, height, chest circumference [4,11]. Copper plays a role in the mobilization of iron to plasma from the tissue stores and copper deficiency during embryonic and foetal development has been found to cause numerous gross structural and biochemical abnormalities. It has been reported that more than 50% of human conception fail to implant and of those implanted, approximately 30% fail to reach term due to copper deficit. Lower plasma copper levels have been found in case of chronic intrauterine hypoxia and fetal hypotrophy, internal organ abnormalities [7,12]. Deficiency of zinc as an essential trace element with wide range of functions including the synthesis of enzymes leads to pregnancy wastage, congenital abnormalities, placental abruption, pregnancy induced hypertension, miscarriage and low birth weight [13,14]. Lower plasma zinc concentrations during the pregnancy is associated with preeclampsia, threatened abortion, premature birth syndrome, birth defects, circulatory disorders, the immune response impairments, and disorders of psychological development [15,16]. In this regard, it is relevant to study the effects of toxic and trace elements on pregnancy through the investigation of their content in women’s blood during uncomplicated and complicated gestation, with further elaboration of methodological algorithms for physiological monitoring of pregnancy for the timely implementation of preventive measures. The aim of this research was to evaluate lead, cadmium, copper and zinc content in women’s blood during III trimester of pregnancy and to assess the correlation between their levels and gestational complications.
{"title":"Lead, cadmium, copper and zinc content in women’s blood during the third trimester of uncomplicated and complicated gestation","authors":"M. Gzhegotskyi, N. Sukhodolska","doi":"10.25040/ecpb2019.03.005","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.005","url":null,"abstract":"In modern realities of adverse effects of ecosystem on human health, WHO experts have proposed a program of research “Human reproduction”, whose main task is to study the negative impact of environmental factors on the generative function [1-3]. Under the high technogenic loading conditions, the problem of harmful influence of heavy metals in combination with the imbalance of trace elements on woman’s organisms, which lead to a decrease in adaptation reserves with the further development of changes in most systems of the organism, is urgent [4-6]. Recent studies have already identified a correlation between the elevated levels of lead in the blood and the development of threatened abortion and premature birth syndrome [7,8]; excess of lead causes the miscarriage, intrauterine growth restriction, chronic fetal hypoxia, birth defects in newborn babies [9,10]. High levels of cadmium in the placenta adversely affect the anthropometric data of the newborn – weight, height, chest circumference [4,11]. Copper plays a role in the mobilization of iron to plasma from the tissue stores and copper deficiency during embryonic and foetal development has been found to cause numerous gross structural and biochemical abnormalities. It has been reported that more than 50% of human conception fail to implant and of those implanted, approximately 30% fail to reach term due to copper deficit. Lower plasma copper levels have been found in case of chronic intrauterine hypoxia and fetal hypotrophy, internal organ abnormalities [7,12]. Deficiency of zinc as an essential trace element with wide range of functions including the synthesis of enzymes leads to pregnancy wastage, congenital abnormalities, placental abruption, pregnancy induced hypertension, miscarriage and low birth weight [13,14]. Lower plasma zinc concentrations during the pregnancy is associated with preeclampsia, threatened abortion, premature birth syndrome, birth defects, circulatory disorders, the immune response impairments, and disorders of psychological development [15,16]. In this regard, it is relevant to study the effects of toxic and trace elements on pregnancy through the investigation of their content in women’s blood during uncomplicated and complicated gestation, with further elaboration of methodological algorithms for physiological monitoring of pregnancy for the timely implementation of preventive measures. The aim of this research was to evaluate lead, cadmium, copper and zinc content in women’s blood during III trimester of pregnancy and to assess the correlation between their levels and gestational complications.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72807024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.25040/ecpb2019.03.037
O. Kutelmakh
{"title":"Effect of Experimental Hypergomocysteinemia and its Correction by Choline and Vitamin D on the Reproductive Function and Dental Status of Rates of Rats","authors":"O. Kutelmakh","doi":"10.25040/ecpb2019.03.037","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.037","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77789923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.25040/ecpb2019.03.061
S. Vadzyuk, R. Shmatа, Ukraine Ternopil
{"title":"Psychophysiological specifics in persons of juvenile age with hight vestibular sensivity","authors":"S. Vadzyuk, R. Shmatа, Ukraine Ternopil","doi":"10.25040/ecpb2019.03.061","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.061","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76711563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.25040/ecpb2019.03.044
M. Lomikovska, I. Kril, V. Chopyak
{"title":"Peculiarities of lymphocytic pool in the blood of reactive arthritis patients with Epstein-Barr viral and chlamydial infections in the background","authors":"M. Lomikovska, I. Kril, V. Chopyak","doi":"10.25040/ecpb2019.03.044","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.044","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81252221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.25040/ecpb2019.03.012
Y. Zavidnyuk, O. Mel’nyk, O. Mysakovets
Introduction. Despite considerable informativeness, factor analysis in biomedical research is still rarely used. Therefore, we set out to introduce our colleagues to the theoretical foundations of factor analysis and to demonstrate its application in our own material. According to the theory of factor analysis [1], it is considered that the observed parameters (variables) are a linear combination of some latent (hypothetical, unobservable) factors. In other words, the factors are hypothetical, not directly measured, hidden variables, in terms of which the measured variables are described. Some of the factors are assumed to be common to two or more variables, while others are specific to each parameter. Characteristic (unique) factors are orthogonal to one another, that is, they do not contribute to the covariance between the variables. In other words, only common factors that are much smaller than the number of variables contribute to the covariance between them. The latent factor structure can be accurately identified by examining the resulting covariance matrix. In practice, it is impossible to obtain the exact structure of the factor model, only estimates of the parameters of the factor structure can be found. Therefore, on the principle of postulate of parsimony, adopt a model with a minimum number of common factors. One of the methods of factor analysis is the analysis of principal components. Principal components (PCs) are linear combinations of observed variables that have orthogonality properties, that is, natural orthogonal functions. Thus, PCs are opposite to common factors, since the latter are hypothetical and are not expressed through a combination of variables, whereas PCs are linear functions of the observed variables. The essence of the PCs method lies in the linear transformation and condensation of the original information. On the basis of correlation matrices, a system of orthogonal, linearly independent functions, nominated by eigenvectors, corresponding to a system of independent random variables nominated by eigenvalues of the correlation matrix (λ) is determined. The first few eigenvalues of the correlation matrix exhaust the bulk of the total field variance, so special attention is given to the first eigenvalues and their corresponding components when analyzing the decomposition results. And since large-scale processes, which are functional systems of the body, are characterized
{"title":"Factor analysis of the information field of the neuroendocrine-immune complex and metabolism in female rats","authors":"Y. Zavidnyuk, O. Mel’nyk, O. Mysakovets","doi":"10.25040/ecpb2019.03.012","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.012","url":null,"abstract":"Introduction. Despite considerable informativeness, factor analysis in biomedical research is still rarely used. Therefore, we set out to introduce our colleagues to the theoretical foundations of factor analysis and to demonstrate its application in our own material. According to the theory of factor analysis [1], it is considered that the observed parameters (variables) are a linear combination of some latent (hypothetical, unobservable) factors. In other words, the factors are hypothetical, not directly measured, hidden variables, in terms of which the measured variables are described. Some of the factors are assumed to be common to two or more variables, while others are specific to each parameter. Characteristic (unique) factors are orthogonal to one another, that is, they do not contribute to the covariance between the variables. In other words, only common factors that are much smaller than the number of variables contribute to the covariance between them. The latent factor structure can be accurately identified by examining the resulting covariance matrix. In practice, it is impossible to obtain the exact structure of the factor model, only estimates of the parameters of the factor structure can be found. Therefore, on the principle of postulate of parsimony, adopt a model with a minimum number of common factors. One of the methods of factor analysis is the analysis of principal components. Principal components (PCs) are linear combinations of observed variables that have orthogonality properties, that is, natural orthogonal functions. Thus, PCs are opposite to common factors, since the latter are hypothetical and are not expressed through a combination of variables, whereas PCs are linear functions of the observed variables. The essence of the PCs method lies in the linear transformation and condensation of the original information. On the basis of correlation matrices, a system of orthogonal, linearly independent functions, nominated by eigenvectors, corresponding to a system of independent random variables nominated by eigenvalues of the correlation matrix (λ) is determined. The first few eigenvalues of the correlation matrix exhaust the bulk of the total field variance, so special attention is given to the first eigenvalues and their corresponding components when analyzing the decomposition results. And since large-scale processes, which are functional systems of the body, are characterized","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81276647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.25040/ecpb2019.03.068
I. Dmytrenko, I. Dyagil, Z. Minchenko, Z. Martina, V. Fedorenko, T. Shlyakhtychenko, V. Sholoyko, O. Dmytrenko
{"title":"The Previous Treatment Value for the Nilotinib Therapy Efficiency in Patients with Chronic Myeloid Leukemia","authors":"I. Dmytrenko, I. Dyagil, Z. Minchenko, Z. Martina, V. Fedorenko, T. Shlyakhtychenko, V. Sholoyko, O. Dmytrenko","doi":"10.25040/ecpb2019.03.068","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.068","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78424317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.25040/ecpb2019.03.030
L. E. Grytcishin, L. Fira, P. Lykhatskyi
{"title":"Status of the Antioxidant System in Rats under the Conditions of Dimethylhydrazine-Induced Changesorogenesis and Application of Citostaitic Therapy","authors":"L. E. Grytcishin, L. Fira, P. Lykhatskyi","doi":"10.25040/ecpb2019.03.030","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.030","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82202090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-15DOI: 10.25040/ecpb2019.03.023
I. Kovalenko, O. K. Onufrovych, O. Melnyk, O. Korchynska, Z. Vorobets, N. Vorobets
An important role in the etiotropic therapy of purulent-inflammatory complications take fluoroquinolones which are active against pathogenic microflora. Due to their wide range of antimicrobial activity, low toxicity, good pharmacokinetics properties, they are widely use in the treatment of bacterial infections of different localization. These are drugs of ultra-wide range of activity, active against gram-positive and gram-negative, aerobic and anaerobic microorganisms, chlamydia, mycoplasma, mycobacteria [1-4]. Fluoroquinolones are synthesized by introducing into the molecule 4-quinolones – one, two or four fluorine atoms [1,2]. These synthetic antibiotics have a specific mechanism of action, which is to inhibit the activity of DNA gyrase (topoisomerase II) and topoisomerase IV of bacterial cells – the enzyme responsible for the stability of the DNA structure of the bacteria and is involved in cell division [2,3,5]. Under the action of fluoroquinolones, the bacterial cell's DNA is despiralized, its spatial structure is disrupted and, as a consequence, the process of replication, transcription, translation, and cell death is impaired. The pharmacodynamics of fluoroquinolones include antibacterial (bacteriocidal), postantibiotic and immunomodulatory effects [7,8]. Drugs 2nd generations (for example, ciprofloxacin) are active in a wide range of gram-negative aerobic microorganisms as well as Staphylococcus aureus, salmonella, Escherichia coli [6]. Also active against mycobacterium tuberculosis. Moderately active in attitude to pneumococci, enterococci, chlamydia. Its bioavailability is 80%. Third-generation fluoroquinolones are highly active against pneumococci and intracellular pathogens, in particular mycoplasma and chlamydia [6]. Thus. levofloxacin has advantages over other fluoroquinolones against pneumococci, chlamydia, mycoplasma. More frequent it is used for the treatment of respiratory infections, as well as infections of the urogenital tract, skin. New, IV generation fluoroquinolones, in particular moxifloxacin have considerably anymore activity against gram-positive microorganisms compared to previous generations of fluoroquinolones [6]. Moxifloxacin is most active against staphylococci, streptococci. In relation to pneumococci, it is in 4-16 times more active than ciprofloxacin. Moxifloxacin acts on polyresistant strains such as chlamydia, mycoplasma, ureaplasma in particular on mycobacterium of tuberculosis, which bioavailability is 90%. It is possible to assume that antibiotics of fluorchinolone if they enter human body also have influence on eukaryotic cells, in particular blood cells. It
{"title":"Effect of Fluoroquinolones on the Activity of the Glutathione System in the Peripheral Blood Lymphocytes","authors":"I. Kovalenko, O. K. Onufrovych, O. Melnyk, O. Korchynska, Z. Vorobets, N. Vorobets","doi":"10.25040/ecpb2019.03.023","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.023","url":null,"abstract":"An important role in the etiotropic therapy of purulent-inflammatory complications take fluoroquinolones which are active against pathogenic microflora. Due to their wide range of antimicrobial activity, low toxicity, good pharmacokinetics properties, they are widely use in the treatment of bacterial infections of different localization. These are drugs of ultra-wide range of activity, active against gram-positive and gram-negative, aerobic and anaerobic microorganisms, chlamydia, mycoplasma, mycobacteria [1-4]. Fluoroquinolones are synthesized by introducing into the molecule 4-quinolones – one, two or four fluorine atoms [1,2]. These synthetic antibiotics have a specific mechanism of action, which is to inhibit the activity of DNA gyrase (topoisomerase II) and topoisomerase IV of bacterial cells – the enzyme responsible for the stability of the DNA structure of the bacteria and is involved in cell division [2,3,5]. Under the action of fluoroquinolones, the bacterial cell's DNA is despiralized, its spatial structure is disrupted and, as a consequence, the process of replication, transcription, translation, and cell death is impaired. The pharmacodynamics of fluoroquinolones include antibacterial (bacteriocidal), postantibiotic and immunomodulatory effects [7,8]. Drugs 2nd generations (for example, ciprofloxacin) are active in a wide range of gram-negative aerobic microorganisms as well as Staphylococcus aureus, salmonella, Escherichia coli [6]. Also active against mycobacterium tuberculosis. Moderately active in attitude to pneumococci, enterococci, chlamydia. Its bioavailability is 80%. Third-generation fluoroquinolones are highly active against pneumococci and intracellular pathogens, in particular mycoplasma and chlamydia [6]. Thus. levofloxacin has advantages over other fluoroquinolones against pneumococci, chlamydia, mycoplasma. More frequent it is used for the treatment of respiratory infections, as well as infections of the urogenital tract, skin. New, IV generation fluoroquinolones, in particular moxifloxacin have considerably anymore activity against gram-positive microorganisms compared to previous generations of fluoroquinolones [6]. Moxifloxacin is most active against staphylococci, streptococci. In relation to pneumococci, it is in 4-16 times more active than ciprofloxacin. Moxifloxacin acts on polyresistant strains such as chlamydia, mycoplasma, ureaplasma in particular on mycobacterium of tuberculosis, which bioavailability is 90%. It is possible to assume that antibiotics of fluorchinolone if they enter human body also have influence on eukaryotic cells, in particular blood cells. It","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90934341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-10DOI: 10.25040/ecpb2019.03.082
A. Trushenko, V. Mamchur
The incidence of inflammatory bowel diseases, which has recently increased, encourages sci-entists to search for the most representative and easily reproducible animal models for studying the links of pathogenesis and possible ways of treating of this pathology. This review presents analysis of the genetically-conditioned and chemically-induced models of inflammatory bowel diseases. There are discussed the most common genetically conditioned models based on defects of the immune system links, loss of components of the intestinal epithelium, or spontaneous lesions of the intestinal tube wall. It is also described the techniques of the most appropriate chemically-induced models, as well as the main pathogenetic links in the development of colitis when using chemical agents. In particular, modeling of Crohn's disease with trinitrobenzenesulfonic acid (TBSA), and ulcerative colitis – with dextran sodium sulfate (DSS), as well as other models having common pathogenetic mechanisms of inflammation in the small and large intestine, e.g. models in which acetic acid, peptidoglycan, λ-carrageenan and indomethacin are used as acting chemical agents. Taking into account the correspondence of the reproduction of pathogenetic signs of inflammatory bowel disease in animals to such in people, the complexity of reproduction and the cost of the necessary components, among the discussed techniques, the undeniable advantages belong to the DSS model of ulcerative colitis and TBSA model of the reproduction of Crohn’s disease, and also the indomethacin model of inflammatory processes of the small intestine. colon, disposition of crypts with the appearance of a wide gate between the crypt base and the muscular layer, and the occurrence of lymphocytosis. TBSA also causes significant changes in the morphological characteristics, mechanical properties and pharmacological response of the circular muscular layer of the distal intestinal regions. TBSA causes a local increase in the synthesis of proinflammatory mediators, which is associated with a neutrophil response, resulting in profound destructive changes in the distal bowel. Finally, indomethacin, which is a strong non-steroidal anti-inflammatory drug with nonspecific action, causes more damage to the distal jejunum and proximal ileum. These models allow reproducing the corresponding basic pathogenetic changes in inflammatory bowel disease, which makes it possible to study the systemic and intersystemic cause-effect relationships of pathobiochemical, histological and pathophysiological levels at different stages of the development of the disease and upon pharmacological correction. Our interest in com-parative analysis of the existing pathological models, selection of the corresponding adequate modelling schemes for use in our conditions is due to further in-depth study of the pathogenesis of inflammatory bowel disease, as well as further research of pharmacological approaches for correction of these pathological conditio
{"title":"Reproduction of inflammatory bowel diseases in experiment","authors":"A. Trushenko, V. Mamchur","doi":"10.25040/ecpb2019.03.082","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.082","url":null,"abstract":"The incidence of inflammatory bowel diseases, which has recently increased, encourages sci-entists to search for the most representative and easily reproducible animal models for studying the links of pathogenesis and possible ways of treating of this pathology. This review presents analysis of the genetically-conditioned and chemically-induced models of inflammatory bowel diseases. There are discussed the most common genetically conditioned models based on defects of the immune system links, loss of components of the intestinal epithelium, or spontaneous lesions of the intestinal tube wall. It is also described the techniques of the most appropriate chemically-induced models, as well as the main pathogenetic links in the development of colitis when using chemical agents. In particular, modeling of Crohn's disease with trinitrobenzenesulfonic acid (TBSA), and ulcerative colitis – with dextran sodium sulfate (DSS), as well as other models having common pathogenetic mechanisms of inflammation in the small and large intestine, e.g. models in which acetic acid, peptidoglycan, λ-carrageenan and indomethacin are used as acting chemical agents. Taking into account the correspondence of the reproduction of pathogenetic signs of inflammatory bowel disease in animals to such in people, the complexity of reproduction and the cost of the necessary components, among the discussed techniques, the undeniable advantages belong to the DSS model of ulcerative colitis and TBSA model of the reproduction of Crohn’s disease, and also the indomethacin model of inflammatory processes of the small intestine. colon, disposition of crypts with the appearance of a wide gate between the crypt base and the muscular layer, and the occurrence of lymphocytosis. TBSA also causes significant changes in the morphological characteristics, mechanical properties and pharmacological response of the circular muscular layer of the distal intestinal regions. TBSA causes a local increase in the synthesis of proinflammatory mediators, which is associated with a neutrophil response, resulting in profound destructive changes in the distal bowel. Finally, indomethacin, which is a strong non-steroidal anti-inflammatory drug with nonspecific action, causes more damage to the distal jejunum and proximal ileum. These models allow reproducing the corresponding basic pathogenetic changes in inflammatory bowel disease, which makes it possible to study the systemic and intersystemic cause-effect relationships of pathobiochemical, histological and pathophysiological levels at different stages of the development of the disease and upon pharmacological correction. Our interest in com-parative analysis of the existing pathological models, selection of the corresponding adequate modelling schemes for use in our conditions is due to further in-depth study of the pathogenesis of inflammatory bowel disease, as well as further research of pharmacological approaches for correction of these pathological conditio","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83427579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-10DOI: 10.25040/ecpb2019.03.075
V. Shevaga, A. Payenok, A. Netliukh, T. Gutor, N. Мatolinets, B. Zadorozhna, O. Kobyletskyi
Special place is taken by nitrogen oxide (NO) gas transmitter. The peculiarities of the NO in trauma are that it is synthesized very quickly in response to the stimuli, and upon release, en-ters the cell by diffusion without requiring receptors to initiate intracellular signals, and then chemically modifies the cellular targets. Important role is noted also for deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). the (GOS): (1 on GOS, patients), (2-3 on GOS, (4 GOS, the dynamics and correlation of and acids (DNA and RNA), that reliably correlated with outcomes. The results of the investigation will be useful for practitioners to assess the patient's condition and choose appropriate treatment approach, which will minimize the secondary brain traumatization and predict the outcomes of TBI.
{"title":"Significance оf Gas Transmitter Nitrogen Oxide аnd Nucleic Acids in the Prognosis for Patients with Severe Brain Injury","authors":"V. Shevaga, A. Payenok, A. Netliukh, T. Gutor, N. Мatolinets, B. Zadorozhna, O. Kobyletskyi","doi":"10.25040/ecpb2019.03.075","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.075","url":null,"abstract":"Special place is taken by nitrogen oxide (NO) gas transmitter. The peculiarities of the NO in trauma are that it is synthesized very quickly in response to the stimuli, and upon release, en-ters the cell by diffusion without requiring receptors to initiate intracellular signals, and then chemically modifies the cellular targets. Important role is noted also for deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). the (GOS): (1 on GOS, patients), (2-3 on GOS, (4 GOS, the dynamics and correlation of and acids (DNA and RNA), that reliably correlated with outcomes. The results of the investigation will be useful for practitioners to assess the patient's condition and choose appropriate treatment approach, which will minimize the secondary brain traumatization and predict the outcomes of TBI.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87452472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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