Fitoterapia最新文献

Nine jatrophane diterpenoids, including six previously undescribed compounds, were extracted and purified from whole plants of Jatropha curcas L. Their structures including absolute configurations were characterized by spectroscopic, quantum chemical Nuclear Magnetic Resonance Spectroscopy, Electronic Circular Dichroism calculation, and Single Crystal X-Ray Diffraction methods. These compounds were evaluated for their ability to reverse multidrug resistance. Among the above compounds, compound 6 showed significant potential as a multidrug resistance modulator, exhibiting higher chemo reversal effects and lower toxicity compared to the positive control verapamil. These findings suggest that compound 6 has promising anticancer properties.

Background: Argan oil (AO) is a vegetable oil extracted from the fruits of Argania spinosa L. tree, belonging to the Sapotaceae family, primarily found in Morocco. Research studies have demonstrated that AO exhibits diverse pharmacological properties, including antioxidant, antimicrobial, anticancer, antiinflammatory, antidiabetic, antihypercholesterolemic, antiatherogenic, and immunomodulatory effects. These effects are attributed to its main constituents, including oleic acid, linoleic acid, γ-tocopherol, α-tocopherol, and ferulic acid.

Objective: This review aimed to present the protective role of AO and its main constituents against xenobiotics-induced toxicities.

Material and methods: Based on results from various in vitro and in vivo investigations published in the main scientific databases, the beneficial action of AO against xenobiotics-induced toxicities was analyzed.

Results: AO and its main constituents have reduced neurotoxicity, hepatotoxicity, nephrotoxicity, pneumotoxicity, thyroid toxicity, hematotoxicity, immunotoxicity, genotoxicity, and colon toxicity induced by different natural and chemical xenobiotics. Different mechanisms of action are involved in these effects, including enhancement of antioxidant defense, reduction of oxidative stress, modulation of inflammation, stimulation of fatty acid oxidation, suppression of apoptosis, regulation of miRNAs expression, elevation of acetylcholinesterase activity, activation of Krebs cycle enzymes, and restoration of mitochondrial function.

Conclusion: The study shows clearly the beneficial effect of Argan oil against xenobiotics-induced toxicities was analyzed. However, clinical trials are necessary to verify the protective effects of this oil in human intoxications caused by both natural and chemical xenobiotics.

Melastoma dodecandrum Lour. is a creeping shrub belonging to the Melastomataceae family. It has been widely used as a medicine and food in South China and is recognized as a safe and effective anti-inflammatory agent. 35 compounds (1-35) were isolated from the whole herbs of M. dodecandrum, including two undescribed polyphenolics (1 and 2), two undescribed triterpenoids (3 and 4), twenty-eight known polyphenolics (5-32) and three known triterpenoids (33-35). Based on chemical evidence and spectral data analysis (UV, Optical rotation data, 1D and 2D-NMR, and HR-ESI-MS), the structures of undescribed compounds were elucidated. Compounds 1-35 were tested for their inhibitory activity on NO production in LPS-induced RAW264.7 cells. Among them, compound 8 exhibited the most excellent inhibitory activity with IC₅₀ value of 14.50 ± 4.69 μM.

Alstoscholarisine L is an architecturally complex monoterpenoid indole alkaloid with a unique ring fusion pattern, isolated from the leaves of Alstonia scholaris. The 6/5/5/6/6/6-membered rings contain two lactonic rings and one aminal carbon and possess seven contiguous aligned stereocenters, three of which are quaternary. Its structure was elucidated by extensive spectroscopic data analyses, quantum chemical computations, and single-crystal X-ray diffraction. The unusual highly fused, cage-like skeleton is possibly derived from picrinine. The fascinating compound exhibited potential antifungal activity against Candida albicans, and its activity was roughly comparable to the first line antifungal drug fluconazole and significantly more effective than the plant-derived antibacterial drug berberine.

In this study have been evaluated the antiproliferative and antioxidant activities of Israeli Pulicaria incisa (Lam.) DC. essential oil (Pi1), of essential oil partially purified by xanthoxylin (Pi2), one of its feature metabolites, and the isolated xanthoxylin (Xan). From the compositional analysis carried out by GC-MS oxygenated monoterpenes class was the main class (71.18 %), with cis-chrysanthenol (55.66 %) as the most abundant components, following by carvotanacetone (11.68 %). Other metabolites represented the second class (20.33 %), being xanthoxylin (15.35 %) the principal metabolites, isolated and characterized by NMR tecniques. Pi1 and Xan showed good antioxidant activity causing a clear reduction in ROS levels and an increase in CAT and SOD activity, while Pi2 showed a reduction in enzymatic activity. Furthermore, Pi1 and Xan demonstrated an apoptotic antiproliferative effect in a dose-dependent manner on solid and hematological tumors, unlike Pi2 which showed lower activity. These data showed that P. incisa EO had interesting antioxidant and antitumor activity and that the biological activities exhibited by the essential oil as a whole are mainly due to xanthoxylin.

The Euphorbia plants are famous for their diterpenoid constituents with diverse structures and broad biological activities. Herein, the discovery of 15 ingenane diterpenoids including 10 previously unreported ones (1-10) from Euphorbia antiquorum was presented. Structures of the undescribed compounds were established via detailed spectroscopic analyses. Meanwhile, a preliminary anti-inflammatory screening revealed that compound 6 showed significant inhibitory activity against the production of nitric oxide, as well as downregulated the expression of COX-2 and IL-6, in lipopolysaccharide-stimulated RAW264.7 macrophages. Further mechanistical exploration revealed that compound 6 could exert its anti-inflammatory effect by inhibiting NF-κB and activating Nrf2 signaling pathways.

The antioxidant activity of Scorzonera parviflora Jacq. roots were assessed by measuring their ability to scavenge ABTS and DPPH radicals. Bioactivity-guided fractionation was utilized to identify the compound(s) responsible for this activity. The most active phase, ethyl acetate, was isolated using column chromatography. The resulting fractions were then purified using preparative TLC on reverse phase and semi-preparative HPLC. The structures of the pure compounds were elucidated by spectral analysis (MS and ¹H, ¹³C, 2D-NMR). Three undescribed phenolic acid derivatives, namely parvifloric acid A (1), B (2), and C (3), and one new sesquiterpene lactone, parviflorin (4) together with seven known compounds were isolated and identified as scopolin (5), scopoletin (6), caffeic acid (7), protocatechuic acid (8), 4,5-O-dicaffeoylquinic acid (9) 3,5-O-dicaffeoylquinic acid (10), and 3,5-O-dicaffeoylquinic acid methyl ester (11). Finally, the pure compounds obtained were tested to evaluate their antioxidant capacities, using ABTS and DPPH radical scavenging potencies. The highest activity was observed with 3,5-O-dicaffeoylquinic acid (10), followed by its methyl ester.

Although Aristolochia plants remain controversial due to their toxicity, this group of perianth-bearing plants, which includes the medicinal species Aristolochia esperanzae, is among the most relevant from an ethnobotanical perspective. All parts of A. esperanzae are used in popular medicine in the form of infusion for the treatment of rheumatism. Thus, the anti-inflammatory properties and the chemical composition of the aqueous extract (AELS) obtained from A. esperanzae aerial parts were examined in this research. AELS was analyzed using chromatographic, hyphenated, spectrometric techniques, and a total of 35 compounds, seven alkaloids, one alkamide, seven flavonoids, four phenolic compounds, one terpene lactone, three epoxylignans, two dibenzylbutyrolactolic, five dibenzylbutyrolactone, three furofuran lignans, and two labdane diterpenes, were found in this extract. Four doses of AELS (3-300 mg/kg) were administered, by oral route (p.o.), to female Swiss mice in the carrageenan pleurisy test. The dose of 100 mg/kg was tested in Swiss mice in two models: carrageenan, complete adjuvant Freunds (CFA) and zymosan inflammatory models. The AELS extract was assayed using the methylthiazolidiphenyl-tetrazolium bromide (MTT) test to verify the leukocyte viability, and this showed no cytotoxicity in vitro. AELS significantly interfered with nitric oxide production and reduced leukocyte migration to the pleura following carrageenan stimulation. AELS significantly inhibited nitric oxide production, leukocyte migration, and mechanical hyperalgesia after zymosan-stimulated joint inflammatory process. Therefore, the present study demonstrated that AELS exhibits anti-inflammatory properties and is rich in compounds known for their anti-inflammatory potential, thereby supporting the traditional use of A. esperanzae in treating rheumatism.

β-Carboline alkaloids are a broad class of indole alkaloids that were first isolated from Peganum harmala L., a traditional Chinese herbal remedy. β-Carboline alkaloids have been found to have many pharmacological activities, including anti-inflammatory, antioxidant, and anti-cancer properties. β-Carboline alkaloids have been studied, and nine therapeutic medications based on its structural skeleton have been utilized to treat a range of illnesses. These compounds' potent pharmacological action and high druggability have garnered a lot of interest. This review systematically summarized resource distribution, pharmacological activity, toxicology and clinical drugs of β-Carboline alkaloids. These alkaloids are mostly found in plants, particularly (Peganum harmala L.), although they are also present in food, bacteria, fungus, and animals. By inhibiting NF-κB, MAPKs, and PI3K-AKT multiple signal pathways, they demonstrate a wide range of pharmacological activities, including anti-inflammatory, oxidative, neurological, cancer, fungal, and leishmania pharmacological activity. Toxicology revealed that β-Carboline alkaloids can produce confusion, irritability, dyskinesia, nausea, vomiting, and audiovisual hallucinations in addition to stimulating the central nervous system and inhibiting metabolism. Clinical drugs based on β-Carboline alkaloids have been used for clinical treatment of arrhythmia, cerebrovascular diseases and dysfunction, hypertension, epilepsy, malaria and mydriasis diseases. It will prompt us to redefine β-Carboline alkaloids. For β-Carboline alkaloids that inspires pharmacological applications in medicine and the development of novel medications containing these alkaloids, it will be a useful resource.