Fitoterapia最新文献

英文中文

Ethyl acetate fraction of Striga hermonthica (Delile) Benth. inhibits AGEs-mediated inflammatory markers in THP-1 monocytes 白刺刺的乙酸乙酯部分。抑制THP-1单核细胞中ages介导的炎症标志物。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-02 DOI: 10.1016/j.fitote.2026.107082

Abdoulaye Segda , Priya Tufail , Roland Nâg-Tiéro Méda , Aneela Fayaz , Benjamin Kouliga Koama , Georges Anicet Ouédraogo , Humera Jahan , M. Iqbal Choudhary

The present study investigated the antiglycation activities of extracts and fractions of Phyllanthus amarus, Chrysanthellum americanum, Striga hermonthica and, based on cytotoxicity results, evaluated the anti-inflammatory potential of the ethyl acetate fraction of Striga hermonthica (EtOAc-Sh) in AGE-challenged THP-1 monocytes. The in vitro methylglyoxal (MGO)-bovine serum albumin (BSA) assay revealed notable inhibition of AGE formation by EtOAc-Sh (IC₅₀ = 100.1 ± 0.001 μg/mL; quercetin 1.23 μM, gallic acid 0.131 μM, rutin 0.0021 μM), with rutin used as the standard (IC₅₀ = 402 ± 0.30 μM). Cell metabolic assay showed EtOAc-Sh was non-cytotoxic to HepG2 hepatocytes (∼ 94 % cell viability at 250 μg/mL), and THP-1 monocytes (≥ 90 % cell viability at 500 μg/mL). Moreover, EtOAc-Sh significantly (p < 0.001) reduced the AGE-mediated ROS production (83 % at 100 μg/mL), as compared to apocynin (69 % at 100 μM). Furthermore, EtOAc-Sh suppressed the NF-κB (p⁶⁵) (RFU: 9.18 at 100 μg/mL) activation, as compared to PDTC (RFU: 6.97) at 100 μM. EtOAc-Sh also significantly (p < 0.001) reduced the COX-2 levels (1.52-fold decrease at 100 μg/mL; PDTC, 1.68-fold decrease) in THP-1 monocytes, while significantly (p < 0.001) reversing the AGE-induced suppression of COX-1 levels (1.89-fold increase at 100 μg/mL; PDTC, 1.88-fold increase) at 100 μM. HPLC-UV analysis identified quercetin, gallic acid, and rutin, as the active constituents of the EtOAc-Sh fraction. These findings suggest that EtOAc-Sh fraction as a potential antiglycation, and anti-inflammatory agent, which supporting the traditional use of Striga hermonthica in diabetes management in Burkina Faso.

还原糖和蛋白质氨基之间的非酶反应形成晚期糖基化终产物（AGEs），这有助于糖尿病的发病。本研究研究了海刺藻乙酸乙酯部分（EtOAc-Sh）在age激发的THP-1单核细胞中的抗糖化和抗炎潜能。体外甲基乙二醛(分别)牛血清白蛋白(BSA)分析显示显著的时代形成的抑制(IC50 =  100.1±0.001  μg / mL;估计 槲皮素1.23μM,没食子酸 0.131μM,芦丁 0.0021μM),用芦丁作为标准(IC50 = 402 ±0.30  μM)。细胞代谢实验显示，EtOAc-Sh对HepG2肝细胞（250 μg/mL时细胞活力≈94 %）和THP-1单核细胞（500 μg/mL时细胞活力≥90 %）无细胞毒性。此外，与PDTC （RFU: 6.97, 100 μM）相比，EtOAc-Sh显著（p 65）（100 μg/mL时RFU: 9.18）活化。EtOAc-Sh也显著(p

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引用次数: 0

Topical application of Calvatia lilacina ethanol extract-derived fraction promotes diabetic wound healing 局部应用丁香花乙醇提取物衍生部分促进糖尿病伤口愈合。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-02 DOI: 10.1016/j.fitote.2026.107087

Yan Li , Tiantian Dong , Tangtang He , Yurong Zhao , Chenxi Yang , Hanlu Xiao , Ruiwen Mo , Jun Chen , Jie Dong

Calvatia lilacina (CL), a traditional Chinese medicinal fungus, has been extensively used for wound healing for centuries. However, the specific effective components of CL remain unclear. This study investigated the phytochemical composition and diabetic wound healing activities of a novel ethanol extract-derived fractions from CL (CLP2). UHPLC-TOF-MS/MS phytochemical analysis revealed 14 active components in CLP2. HPLC analysis showed that ergosterol was the most prevalent component in CLP2, with a content of 837.3 μg/mg. CLP2 significantly accelerated wound healing in db/db diabetic mice, facilitated re-epithelialization and inhibited the polarization of M1 macrophages, downregulated the transcription levels of the inflammatory cytokines, and promoted the expression of anti-inflammatory factors. In vitro tests indicated that CLP2 can promote the proliferation and migration of mouse skin fibroblasts, suppress the expression of M1 macrophage-associated pro-inflammatory cytokines, and enhance M2 polarization. Additionally, in vivo and in vitro experiments confirmed that CLP2 accelerated diabetic wound healing by suppressing M1 macrophages and promoting the polarization of macrophages towards the M2 phenotype, facilitating a faster transition from the inflammatory stage to the proliferative stage. These results highlight CLP2 as a potential therapeutic intervention for diabetic wound healing.

丁香花（calvara lilacina， CL）是一种传统的中药真菌，几个世纪以来一直被广泛用于伤口愈合。然而，CL的具体有效成分尚不清楚。本研究研究了一种新型乙醇提取物（CLP2）的植物化学成分和糖尿病创面愈合活性。UHPLC-TOF-MS/MS植物化学分析显示，CLP2中有14种有效成分。HPLC分析显示，麦角甾醇是CLP2中含量最高的成分，含量为837.3 μg/mg。CLP2显著加速db/db糖尿病小鼠创面愈合，促进M1巨噬细胞再上皮化，抑制M1巨噬细胞极化，下调炎症因子转录水平，促进抗炎因子表达。体外实验表明，CLP2能促进小鼠皮肤成纤维细胞的增殖和迁移，抑制M1巨噬细胞相关的促炎细胞因子的表达，增强M2极化。此外，体内和体外实验证实，CLP2通过抑制M1巨噬细胞，促进巨噬细胞向M2表型极化，促进炎症期向增殖期更快过渡，从而加速糖尿病创面愈合。这些结果突出了CLP2作为糖尿病伤口愈合的潜在治疗干预。

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引用次数: 0

Gu-Jin-Xiao-Ji Decoction potentiates anti-PD-1 immunotherapy in lung cancer by inhibiting the PI3K/AKT/NF-κB/PD-L1 axis 骨进消积汤通过抑制PI3K/AKT/NF-κB/PD-L1轴增强肺癌抗pd -1免疫治疗。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-02 DOI: 10.1016/j.fitote.2026.107085

Yuxin Xie , Gaoyang Lin , Chuanlong Guo , Jin Tian , Lin Long , Lili Zhao , Longjiang Huang , Hongmei Wei , Jun Xiao , Xuemao Liu

This study aims to elucidate the molecular mechanisms by which the traditional Chinese medicine Gu-Jin-Xiao-Ji Decoction (GJXJD) enhances the anti-tumor efficacy of PD-1 inhibitors in lung cancer and to characterize its major chemical components. The major components of GJXJD were identified and quantitatively analyzed using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). A mouse Lewis lung carcinoma (LLC) subcutaneous xenograft model was established, and high-throughput transcriptome sequencing was performed. Combined treatment with GJXJD and a PD-1 inhibitor significantly suppressed tumor growth, achieving a tumor inhibition rate of 54.48 %, which was substantially higher than that of either monotherapy (GJXJD: 27.58 %; anti-PD-1: 25.76 %). Transcriptomic analysis indicated notable enrichment and downregulation of the PI3K/AKT signaling pathway. Immunohistochemistry revealed inhibition of the PI3K/AKT/NF-κB axis and subsequent downregulation of PD-L1 expression. GJXJD treatment increased spleen and thymus indices, reduced serum IL-17 levels by approximately 40 %, and promoted the secretion of IL-2, IFN-γ, and TNF-α by about 1.5- to 2-fold. Enhanced infiltration of CD3⁺, CD4⁺, and CD8⁺ T cells were observed in tumor tissues, with CD8⁺ T cell infiltration increasing by over 3-fold in the combination group. H&E staining showed no significant pathological changes in major organs. These findings demonstrate that GJXJD potentiates the anti-tumor effect of PD-1 inhibitors by inhibiting the PI3K/AKT/NF-κB pathway, reducing PD-L1 expression, and ameliorating immune evasion. The identified active components provide a chemical basis for its efficacy, supporting GJXJD as a promising adjuvant agent for lung cancer immunotherapy.

本研究旨在阐明中药骨劲消积汤（GJXJD）增强肺癌PD-1抑制剂抗肿瘤作用的分子机制，并对其主要化学成分进行表征。采用超高效液相色谱-串联质谱（UPLC-MS/MS）对GJXJD的主要成分进行了鉴定和定量分析。建立小鼠Lewis肺癌（LLC）皮下异种移植物模型，并进行高通量转录组测序。GJXJD与PD-1抑制剂联合治疗可显著抑制肿瘤生长，肿瘤抑制率为54.48 %，显著高于单药治疗（GJXJD: 27.58 %；抗PD-1: 25.76 %）。转录组学分析显示PI3K/AKT信号通路显著富集和下调。免疫组化显示PI3K/AKT/NF-κB轴受到抑制，随后PD-L1表达下调。GJXJD治疗增加脾脏和胸腺指数，降低血清IL-17水平约40% %，促进IL-2、IFN-γ和TNF-α分泌约1.5- 2倍。肿瘤组织中CD3+、CD4+、CD8+ T细胞浸润增强，联合用药组CD8+ T细胞浸润增加3倍以上。H&E染色未见主要脏器明显病理改变。这些结果表明，GJXJD通过抑制PI3K/AKT/NF-κB通路，降低PD-L1表达，改善免疫逃避，从而增强PD-1抑制剂的抗肿瘤作用。所鉴定的活性成分为其疗效提供了化学基础，支持GJXJD作为肺癌免疫治疗的有前景的佐剂。

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引用次数: 0

Corrigendum to “Anti-diabetic compounds from the seeds of Psoralea corylifolia” [Fitoterapia 139 (2019) 104373] “补骨脂种子抗糖尿病化合物”的勘误表[Fitoterapia 139(2019) 104373]。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-01 DOI: 10.1016/j.fitote.2025.107036

Gaohui Zhu, Yanhong Luo, Xuejiao Xu, Huijiao Zhang, Min Zhu

引用次数: 0

Corrigendum to “Pharmacodynamic effects and mechanism of Pueraria lobata-Schisandra chinensis combination in the treatment of alcoholic liver disease” [Fitoterapia 187 (2025) 106873] “葛根-五味子联合治疗酒精性肝病的药效学作用及机制”的勘误表[Fitoterapia 187(2025) 106873]。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-01 DOI: 10.1016/j.fitote.2025.106991

Shihao Zhang , Ding Liu , Dongyan Guo , Chongbo Zhao , Yajun Shi , Junbo Zou , Huanxian Shi , Jiangxue Cheng , Jing Sun , Xiaofei Zhang

引用次数: 0

Corrigendum to “Isolation and characterization of xuetonlignans L-P, previously undescribed spirobenzofuranoid dibenzocyclooctadiene lignans from the fruits of Kadsura heteroclita” [Fitoterapia 185 (2025) 106726] “从Kadsura heteroclita果实中分离和表征雪桐木脂素L-P，先前描述的螺苯并呋喃二苯并环二烯木脂素”的更正[Fitoterapia 185(2025) 106726]。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-01 DOI: 10.1016/j.fitote.2025.107037

Wei Su , Hanwen Yuan , Muhammad Aamer , Xudong Zhou , Kang Zhou , Ling Li , Yupei Yang , Qingling Xie , Shiqi Liu , Gang Fu , Yu Mao , Bin Li , Wei Wang

引用次数: 0

Corrigendum to “Chemical profiling of Lycium shawii via RP-HPLC-QTOF-MS and MS/MS: Unveiling its in-vivo wound-healing potential supported by molecular docking investigations” [Fitoterapia 185 (2025) 106749] “通过RP-HPLC-QTOF-MS和MS/MS分析枸杞的化学特征：揭示其体内伤口愈合潜力的分子对接研究”的更正[Fitoterapia 185(2025) 106749]。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-01 DOI: 10.1016/j.fitote.2025.106953

Mahmoud Moustafa , Walaa S. Aboelmaaty , Weaam Ebrahim , Reham Hassan Mekky , Mohamed A. Tammam , Amr El-Demerdash , Ahmed M. Zaghloul

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Corrigendum to “Bushen Yinao pill improves cognitive function in Alzheimer's disease rats by regulating PI3K/Akt pathway and intestinal microbiota” [Fitoterapia 188 (2026) 106999] 补肾益脑丸通过调节PI3K/Akt通路和肠道菌群改善阿尔茨海默病大鼠认知功能[Fitoterapia 188(2026) 106999]的更正。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-01 DOI: 10.1016/j.fitote.2025.107019

Shunfeng Lan , Fan Gao , Rong Ji, Zixuan Wang, Zhikun Zang, Yuxin Wei, Haixue Kuang, Zhibin Wang

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Gardenia jasminoides Ellis polysaccharides improve gut microbiota disorder and glucose homeostasis in type 2 diabetic mice 栀子多糖改善2型糖尿病小鼠肠道菌群紊乱和葡萄糖稳态。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2026-01-01 DOI: 10.1016/j.fitote.2025.107080

Wenye Lu , Wanjun Ju , Ningning Niu , Yuanyuan Li , Tianming Wang , Yu Feng , Yueming Ma , Jiasheng Wu

Type 2 diabetes mellitus (T2DM) is the most prevalent metabolic disorder. Gardenia jasminoides Ellis is a traditional herbal medicine used in metabolic disease treatment; however, the effects of Gardenia jasminoides Ellis polysaccharides (GPS) on T2DM remain unknown. This study aimed to investigate the antidiabetic effect of GPS in a T2DM mouse model induced by high-fat diet (HFD) and streptozotocin administration, and to elucidate the potential molecular mechanism. Treatment with low and high doses (200 and 400 mg/kg) of GPS significantly reduced the fasting blood glucose level of T2DM mice by 44.8 % and 33.0 %, respectively. In T2DM mice, oral glucose tolerance test outcomes and insulin tolerance were significantly improved by GPS intervention (p < 0.05), and the pancreatic tissue damage was ameliorated compared with control mice. Moreover, GPS intervention upregulated the liver expression of peroxisome proliferator-activated receptor gamma (PPARγ) by 1.7 fold, improved insulin resistance, and increased glycogen synthesis in T2DM mice compared with in untreated control mice (p < 0.05). More importantly, GPS intervention significantly improved gut microbiota dysbiosis, increased the abundance of short chain fatty acids producing Lactobacillus and Dubosiella by 6.6- and 16.5-fold, respectively, and elevated the serum level of butyric acid in T2DM mice. Furthermore, in vitro experiments confirmed that butyric acid can induce PPARγ expression. In conclusion, these findings suggest that GPS may alleviate T2DM by reshaping the gut microbiota and regulating intestinal metabolites. Therefore, GPS may be a potential prebiotic for modulating T2DM-associated metabolic disorders.

2型糖尿病（T2DM）是最常见的代谢紊乱。栀子是治疗代谢性疾病的传统中草药；然而，栀子多糖（GPS）对T2DM的影响尚不清楚。本研究旨在探讨GPS在高脂饮食（HFD）和链脲佐菌素诱导的T2DM小鼠模型中的降糖作用，并探讨其可能的分子机制。低剂量和高剂量（100和200 mg/kg）的GPS治疗可使T2DM小鼠的空腹血糖水平分别降低44.8% %和33.0% %。在T2DM小鼠中，GPS干预显著改善了口服糖耐量试验结果和胰岛素耐量(p

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Phytochemical constituents and toxicological, anti-inflammatory, and antinociceptive assessment of Baliospermum effusum Pax et Hoffm. 积液巴利ospermum effusum Pax et Hoffm.植物化学成分及毒理学、抗炎和抗伤性评价。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-31 DOI: 10.1016/j.fitote.2025.107078

Qing-Ying Hong, Zu-Hui Wang, Yi-Hang Ba, Yi-Huan Li, De-Xin Li, Hong-Ping He, Rui Yang, Hua-Yi Jiang

Baliospermum effusum Pax et Hoffm., a shrub known as “Baodongdian” in Dai folk medicine, has been traditionally employed for treating traumatic injuries, rheumatism, and jaundice. The present study investigated the acute toxicity, anti-inflammatory and antinociceptive properties, and phytochemical constituents of the 95 % ethanolic extract of B. effusum (EEBe). The toxicity assessment revealed that EEBe (2000 mg/kg) induced no adverse effects in behavior, mortality, body/organ weights, histopathology, or biochemical parameters over 14 d. Pharmacological assays on EEBe (150, 300, and 600 mg/kg) revealed that it effectively inhibited carrageenan-induced paw edema (n = 10), xylene-stimulated ear edema (n = 8), and acetic acid-triggered writhes (n = 8), while showing no significant antinociceptive activity in the hot-plate test (n = 8). ELISA (n = 8) showed that EEBe modulated serum IL-1β, IL-6, and IL-10 levels, while qRT-PCR (n = 5) demonstrated the downregulation of IL-1β and IL-6 mRNA expression. Furthermore, eight compounds including 4-hydroxy-2,3-dimethyl-2-nonen-4-olide (1), 22-dehydroclerosterol (2), β-sitosterol (3), vanillin (4), 1-linolenoylglycerol (5), 1-O-stearoyl-glycerol (6), bis(2,3-dihydroxypropyl)nonanedioate (7), and (3S,5S)-diverniciaoid A (8) were isolated from EEBe, with compounds 5 and 7 suppressing NO production in LPS-induced RAW 264.7 macrophages. These data revealed that EEBe was non-toxic at 2000 mg/kg and exhibited anti-inflammatory and antinociceptive properties, and that 5 and 7, reported for the first time from this species, showed anti-inflammatory activity in vitro, collectively supporting the folk use of B. effusum for inflammatory and pain-related disorders. To our knowledge, this is the first report on the in vivo pharmacological activities and toxicity of B. effusum.

Pax et Hoffm。在傣族民间医学中，这种灌木被称为“宝洞店”，传统上被用来治疗外伤、风湿病和黄疸。本文研究了95 %乙醇提取物的急性毒性、抗炎、抗伤性和植物化学成分。毒性评估显示,EEBe(2000 毫克/公斤)诱导行为,没有不利影响死亡率,阀体/器官重量、组织病理学、或生化参数超过14 d。药理分析EEBe(150、300和600 毫克/公斤)透露,它有效地抑制carrageenan-induced爪子水肿(n = 10),xylene-stimulated耳朵水肿(n = 8)和醋酸acid-triggered扭动(n = 8),而没有显著的抗antinociceptive活动(n = 8)。ELISA （n = 8）结果显示，EEBe可调节血清IL-1β、IL-6、IL-10水平，qRT-PCR （n = 5）结果显示，ebe可下调血清IL-1β、IL-6 mRNA表达。此外，从EEBe中分离到8个化合物，包括4-羟基-2,3-二甲基-2-壬烯-4-olide(1)、22- dehydroclero甾醇(2)、β-谷甾醇(3)、香兰素(4)、1-亚麻烯酰甘油(5)、1- o -硬脂酰甘油(6)、双（2,3-二羟丙基）壬烯二酸酯(7)和(3S,5S)-diverniciaoid A(8)，其中化合物5和7抑制lps诱导的RAW 264.7巨噬细胞NO的产生。这些数据表明，EEBe在2000 mg/kg时无毒，具有抗炎和抗伤性，并且首次从该物种中报道的5和7在体外显示出抗炎活性，共同支持民间将积液芽孢杆菌用于炎症和疼痛相关疾病。据我们所知，这是第一个关于积液芽孢杆菌体内药理活性和毒性的报道。

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