Fitoterapia最新文献_第2页

Synthesis, biological evaluation and mechanism study based on network pharmacology of amino acids esters of 20(S)-protopanaxadiol as novel anticancer agents. 基于网络药理学的 20(S)-protopanaxadiol 氨基酸酯类新型抗癌剂的合成、生物学评价和机理研究。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-11-11 DOI: 10.1016/j.fitote.2024.106274

Hongliu Xie, Fang Lin, Fei Shi, Elaine Johnstone, Yaqi Wang, Yang An, Jun Su, Jiayin Liu, Qinghai Dong, Jihua Liu

As one of the metabolites of ginseng, 20(S)-protopanaxadiol (PPD) is a compound with dammarane-type tetracyclic triterpene, which performs a wide range of anticancer activities. In this study, PPD was used as a lead. A series of compounds were synthesized respectively with 11 amino acids through esterification and were evaluated for their cytotoxicity against several cancer cell lines. One of the synthetic products (PL) exhibited potent inhibitory effect on Huh-7 cells relative to that of PPD in vitro. Subsequently, the Annexin V-FITC /PI staining assay was used to verify that PL induced apoptosis of Huh-7 cells in a dose-dependent manner. A UPLC-Q/TOF-MS analysis method was established and validated for assessing pharmacokinetic properties after the administration of PPD and PL in rats. The results showed that compared with PPD, T_1/2of PL in rats was prolonged, and the peak time was delayed, resulting in broader tissue distribution of the compound in the body. In addition, the targets of PL against several cancers were predicted and analyzed via network pharmacology. Molecular docking simulations demonstrated that PL interacted with the active sites of the above targets. In conclusion, this study provided a theoretical basis for the development and clinical application of anti-tumor activity of PPD.

作为人参的代谢产物之一，20(S)-原人参二醇（PPD）是一种具有达玛烷型四环三萜的化合物，具有广泛的抗癌活性。本研究以 PPD 为先导。通过酯化反应，分别与 11 个氨基酸合成了一系列化合物，并评估了它们对几种癌细胞株的细胞毒性。与 PPD 相比，其中一种合成产物（PL）在体外对 Huh-7 细胞有很强的抑制作用。随后，利用Annexin V-FITC /PI染色法验证了PL诱导Huh-7细胞凋亡的作用呈剂量依赖性。建立并验证了 UPLC-Q/TOF-MS 分析方法，用于评估大鼠服用 PPD 和 PL 后的药代动力学特性。结果表明，与PPD相比，PL在大鼠体内的T1/2延长，达峰时间延迟，从而使该化合物在体内的组织分布更广。此外，还通过网络药理学预测和分析了PL对几种癌症的作用靶点。分子对接模拟表明，PL 与上述靶点的活性位点相互作用。总之，这项研究为 PPD 抗肿瘤活性的开发和临床应用提供了理论依据。

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引用次数: 0

Development of therapeutic and cosmetic cream based on flavonoids 开发基于黄酮类化合物的药用和美容膏霜。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-11-01 DOI: 10.1016/j.fitote.2024.106283

S.M. Adekenov, D.L. Savchenko, A.O. Kenzhebekov, A.N. Zhabaeva, A.S. Adekenova, V.V. Polyakov

The article discusses the results of the development of a cream based on Populus balsamifera L. bud flavonoids, which have anti-inflammatory, skin-regenerating effects.

A cream's anti-inflammatory and wound-healing activity based on a flavonoid substance has been studied. In an experiment on a model of an cut skin wound, it was determined that a cream based on the sum of flavonoids of Populus balsamifera L. buds, containing pinostrobin, pinocembrin, tectochrysin, exhibits anti-inflammatory and wound-healing activity. Considering that the effectiveness of local wound treatment mainly depends on the rational treatment of the first phase of the wound process, we have developed a 1 % flavonoid cream and its production technology.

The technology for the production of flavonoid cream includes extraction and isolation of the sum of flavonoids from the raw material of Populus balsamifera L. buds, preparation of flavonoid substance and excipients, preparation of the cream base and cream, packaging and labelling of cream. Based on the results of our experiments, a pilot industrial regulation for the production of a new cream based on the sum of flavonoids of Populus balsamifera L. buds has been developed, and pilot production of an original therapeutic and cosmetic cream was organised.

文章讨论了基于具有消炎和皮肤再生作用的杨梅花蕾类黄酮的面霜的开发成果。研究了一种基于黄酮类物质的药膏的消炎和伤口愈合活性。在一个皮肤切口模型实验中，确定了一种基于苦杨树花蕾中黄酮类物质总和的药膏，其中含有pinostrobin、pinocembrin和tectochrysin，具有消炎和伤口愈合活性。考虑到局部伤口治疗的效果主要取决于伤口第一阶段的合理治疗，我们开发了一种 1 % 的类黄酮乳膏及其生产技术。类黄酮乳膏的生产技术包括从杨树花蕾原料中提取和分离类黄酮总和、制备类黄酮物质和辅料、制备乳膏基质和乳膏、乳膏的包装和标签。根据我们的实验结果，制定了基于杨树花蕾类黄酮总和生产新型膏霜的试验性工业规程，并组织了原创药用化妆品膏霜的试验性生产。

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引用次数: 0

Garcinia flavonoids for healthy aging: Anti-senescence mechanisms and cosmeceutical applications in skin care 促进健康老龄化的藤黄类化合物：抗衰老机制和药用护肤品的应用。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-11-01 DOI: 10.1016/j.fitote.2024.106282

Idris Adewale Ahmed , Nor Hisam Zamakshshari , Maryam Abimbola Mikail , Ibrahim Bello , Md. Sanower Hossain

Cellular senescence, the irreversible arrest of cell division, is a hallmark of aging and a key contributor to age-related disorders. Targeting senescent cells represents a promising therapeutic approach to combat these ailments. This review explores the potential of Garcinia species, a genus rich in flavonoids with established antioxidant and anti-inflammatory properties, as a source of natural anti-senescence agents. We investigate the intricate connections between aging, cellular senescence, and oxidative stress, highlighting the detrimental effects of free radicals on cellular health. Furthermore, we analyze the diverse array of flavonoids identified within Garcinia and their established cellular mechanisms. We critically evaluate the emerging evidence for the anti-senescence potential of flavonoids in general and the limited research on Garcinia flavonoids in this context. By identifying existing knowledge gaps and paving the way for future research, this review underscores the exciting potential of Garcinia flavonoids as natural anti-senescence agents. These agents hold promise for not only promoting healthy aging but also for the development of cosmeceutical products that combat the visible signs of aging.

细胞衰老是细胞分裂不可逆转的停滞，是衰老的标志，也是导致老年相关疾病的关键因素。以衰老细胞为靶点是一种很有前景的治疗方法。藤黄属植物富含黄酮类化合物，具有公认的抗氧化和抗炎特性，本综述探讨了藤黄属植物作为天然抗衰老剂来源的潜力。我们研究了衰老、细胞衰老和氧化应激之间错综复杂的联系，强调了自由基对细胞健康的有害影响。此外，我们还分析了在藤黄属植物中发现的各种类黄酮及其既定的细胞机制。我们批判性地评估了黄酮类化合物抗衰老潜力的新证据，以及在此背景下有关藤黄属植物黄酮类化合物的有限研究。通过确定现有的知识差距和为未来研究铺平道路，本综述强调了加西黄酮类化合物作为天然抗衰老剂的令人兴奋的潜力。这些制剂不仅有望促进健康老龄化，而且有望开发出能消除明显衰老迹象的药用化妆品。

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引用次数: 0

Biochemometric-guided isolation of new Isosteroidal alkaloids from Fritillaria cirrhosa D.Don (Liliaceae, syn. Fritillaria roylei Hook) as acetylcholinesterase inhibitors. 从 Fritillaria cirrhosa D.Don (Liliaceae, syn. Fritillaria roylei Hook) 中以生物化学计量学为指导分离出新的异甾体生物碱作为乙酰胆碱酯酶抑制剂。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-10-29 DOI: 10.1016/j.fitote.2024.106279

Prateek Singh Bora, Shivani Puri, Prithvi Pal Singh, Upendra Sharma

Globally, Alzheimer's disease is an urgent public health concern with the ageing population in developing nations. Recent studies have identified isosteroidal alkaloids as promising therapeutic agents for Alzheimer's treatment. Fritillaria species are well-known rich sources of steroidal and isosteroidal alkaloids. In this context, the current study focuses on the biochemometric-guided isolation of three previously undescribed and two known isosteroidal alkaloids as acetylcholinesterase (AChE) inhibitors from the bulbs of Fritillaria cirrhosa D.Don. The isolated molecules were characterized by NMR, HR-ESI-MS, FT-IR, and DP4+ analysis. Subsequently, all isolates were evaluated for AChE inhibitory activity using Ellman's method. Among the evaluated molecules, 1 (IC₅₀: 33.0 ± 4.4 μM) and 5 (IC₅₀: 24.7 ± 4.5 μM) showed promising AChE inhibition in vitro. Enzyme kinetic studies of isolated molecules revealed mixed inhibition kinetics with K_i varying from 1.3 to 24.4 μM. Moreover, the in silico studies showed excellent binding affinities of isolated molecules with the target protein and good drug-like ADMET properties. The present study identified new isosteroidal alkaloids as promising AChE inhibitors from F. cirrhosa bulbs via a biochemometric approach and advocated their further exploration for treating neurodegenerative disorders.

在全球范围内，随着发展中国家人口的老龄化，阿尔茨海默氏症已成为一个紧迫的公共卫生问题。最近的研究发现，异甾体生物碱是治疗老年痴呆症的有前途的药物。众所周知，鱼腥草物种富含类固醇和异类固醇生物碱。在此背景下，本研究侧重于在生物化学计量学的指导下，从 Fritillaria cirrhosa D.Don 的鳞茎中分离出三种以前未曾描述过的异甾体生物碱和两种已知的异甾体生物碱，作为乙酰胆碱酯酶（AChE）抑制剂。分离出的分子通过核磁共振、HR-ESI-MS、傅立叶变换红外光谱和 DP4+ 分析进行了表征。随后，采用埃尔曼法评估了所有分离物的 AChE 抑制活性。在评估的分子中，1（IC50：33.0 ± 4.4 μM）和 5（IC50：24.7 ± 4.5 μM）在体外显示出良好的 AChE 抑制作用。对分离的分子进行的酶动力学研究显示了混合抑制动力学，Ki 从 1.3 μM 到 24.4 μM 不等。此外，硅学研究表明，分离出的分子与靶蛋白有极好的结合亲和力，并具有良好的类药物 ADMET 特性。本研究通过生物化学计量学方法从 F. cirrhosa 鳞茎中发现了新的异甾体生物碱作为有前景的 AChE 抑制剂，并主张进一步探索其在治疗神经退行性疾病方面的应用。

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引用次数: 0

Isosativene and sativene sesquiterpene derivatives from Dendrobium nobile 来自金钗石斛的 Isosativene 和 sativene 倍半萜衍生物。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-10-28 DOI: 10.1016/j.fitote.2024.106281

Wei-Wei Fan , Dan Yang , Zhong-Quan Cheng , Liu Yang , Hui-Yan Shao , Xiao-Nian Li , Feng-Qing Xu , Jiang-Miao Hu

Two unusual isosativene sesquiterpene derivatives, named dendronobilol A (1) and dendronobilside A (2), and two unusual sativene sesquiterpene derivatives, named dendronobilsides B (3) and C (4), had been isolated from the stems of Dendrobium nobile. The structures of all the compounds were established using spectroscopic methods and by comparison with literature data, and their absolute configurations were confirmed via single-crystal X-ray diffraction data and electronic circular dichroism (ECD) calculations. Dendronobilol A (1) and dendronobilside A (2) possessed a unique tricyclo[4.3.0.1^{2, 8}]decan ring system, while dendronobilsides B (3) and C (4) presented a unique tricyclo[4.4.0.0^{2, 8}]decan core carbon skeleton. The above two types of sesquiterpene derivatives had been isolated and purified from plants for the first time. Compounds 1 and 2 exhibited significant effects on glucose consumption with doses of 20 and 40 μmol/L in insulin-resistant HepG2 cells, and thus improve insulin resistance.

从金钗石斛的茎中分离出了两种不同寻常的异亚梓倍半萜衍生物，分别命名为树枝烯醇 A (1) 和树枝烯侧 A (2)，以及两种不同寻常的亚梓倍半萜衍生物，分别命名为树枝烯侧 B (3) 和树枝烯侧 C (4)。通过光谱方法以及与文献数据的比较，确定了所有化合物的结构，并通过单晶 X 射线衍射数据和电子圆二色性（ECD）计算确认了它们的绝对构型。Dendronobilol A (1) 和 dendronobilside A (2) 具有独特的三环[4.3.0.12, 8]癸烷环系统，而 dendronobilsides B (3) 和 C (4) 则呈现独特的三环[4.4.0.02, 8]癸烷核心碳骨架。上述两种倍半萜衍生物是首次从植物中分离和纯化出来的。化合物 1 和 2 对胰岛素抵抗的 HepG2 细胞的葡萄糖消耗有显著影响，剂量分别为 20 和 40 μmol/L，从而改善了胰岛素抵抗。

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引用次数: 0

Polygala tenuifolia root extract attenuates ischemic stroke by inhibiting HMGB1 trigger neuroinflammation 远志根提取物通过抑制 HMGB1 引发的神经炎症减轻缺血性中风的症状

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-10-28 DOI: 10.1016/j.fitote.2024.106280

Pingping Shen , Libang Zhang , Xuewa Jiang , Richa Raj , Boyang Yu , Jian Zhang

Polygala tenuifolia Willd., a famous traditional Chinese medicine, has been widely applied to treat central nervous system diseases. In this study, P. tenuifolia root extract exhibited a moderate anti-ischemic effect on in-vitro oxygen-glucose deprivation/reperfusion (OGD/R) model. In transient middle cerebral artery occlusion (tMCAO) rats, P. tenuifolia root extract significantly attenuated brain infarction and neurological deficits in a dose-dependent manner. Compared with the sham group, the release of damage-associated molecular patterns (DAMPs)-HMGB1 in the ischemic brain was significantly higher, which was inhibited by P. tenuifolia root extract. To further explore such neuroprotective effects whether associated with aseptic inflammation, HMGB1-activated BV2 microglial cells model was established. The extract of P. tenuifolia was found to inhibit the downstream inflammatory response driven by HMGB1, with an IC₅₀ value of 49.46 μg/mL. In addition, the extract was also found to be able to directly interact with HMGB1 in the surface plasmon resonance (SPR) experiment. Phytochemical studies showed that the extract of P. tenuifolia root contains a large number of terpenoids, oligosaccharides and phenolic compounds, which likely contribute to the above observed biological activities. Our results not only provide some data support for the clinical application of P. tenuifolia against cerebral ischemia, but also clarify the potential target of P tenuifolia's anti-inflammatory properties.

远志是一种著名的传统中药，被广泛应用于治疗中枢神经系统疾病。本研究发现，远志根提取物在体外氧-葡萄糖剥夺/再灌注（OGD/R）模型中表现出中等程度的抗缺血作用。在瞬时大脑中动脉闭塞（tMCAO）大鼠中，天南星根提取物以剂量依赖的方式显著减轻脑梗塞和神经功能缺损。与假组相比，缺血脑中损伤相关分子模式（DAMPs）-HMGB1的释放量明显升高，而天南星根提取物可抑制DAMPs的释放。为了进一步探讨这种神经保护作用是否与无菌性炎症有关，我们建立了 HMGB1 激活的 BV2 微神经胶质细胞模型。研究发现，白头翁根提取物能抑制由 HMGB1 驱动的下游炎症反应，其 IC50 值为 49.46 μg/mL。此外，在表面等离子体共振（SPR）实验中还发现该提取物能与 HMGB1 直接相互作用。植物化学研究表明，P. tenuifolia 根提取物中含有大量萜类化合物、低聚糖和酚类化合物，这些化合物很可能有助于上述生物活性的观察。我们的研究结果不仅为防脑缺血的临床应用提供了一些数据支持，而且还明确了白头翁抗炎特性的潜在靶点。

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引用次数: 0

Rare crocins ameliorate thrombus in zebrafish larvae by regulating lipid accumulation and clotting factors 稀有鳄鱼毒素通过调节脂质积累和凝血因子改善斑马鱼幼体的血栓形成。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-10-28 DOI: 10.1016/j.fitote.2024.106278

Guo Xu , Penghong Xu , Nan Wang , Weimin Qi , Yuxuan Pu , Nannan Kang , Jianlin Chu , Bingfang He

Crocin-4 is a water-soluble carotenoid that exhibits cardiovascular protection effects through anti-inflammatory and antioxidant effects. However, the pharmacodynamic effects and mechanisms of its analogues crocin-1 and crocin-2′ have not been reported. In this study, we evaluated the protective effects of rare crocins on cardiovascular systems. In ox-LDL induced HUVECs model, 0.02, 0.1, 0.5, 1, 2, 3, 4, 5, 6 μg/mL crocin-1 and crocin-2′ can increase cell viability by up to 80 %. Meanwhile, rare crocins at concentrations between 25–100 μg/mL crocin-1 and crocin-2′ reduced the lipid accumulation by 30 % in cholesterol-induced zebrafish larvae. What's more, the therapeutic potential of rare crocins on thrombosis has also been explored. In vitro experiments, rare crocin-1 and crocin-2′ at concentrations of 0.02, 0.05, 0.2, 0.5, 1, 2, 5, 10 μg/mL protected Human Umbilical Vein Endothelial Cells (HUVECs) against lipopolysaccharides-induced oxidative stress and inflammation. In vivo studies revealed that rare crocins at concentrations of 25, 50, 100, 150, 200, and 300 μg/mL exerted significant antithrombotic effect on arachidonic acid (AA)-induced zebrafish and there was nearly no potential risk for the deformity of zebrafish at 300 μg/mL dosages. In brief, rare crocins was viewed as a potentially useful candidate for the treatment of cardiovascular diseases because of its anti-inflammatory, antioxidant, and anticoagulant properties.

类胡萝卜素-4 是一种水溶性类胡萝卜素，具有抗炎和抗氧化作用，对心血管有保护作用。然而，其类似物黄花素-1 和黄花素-2'的药效学效应和机制尚未见报道。在这项研究中，我们评估了稀有鳄鱼毒素对心血管系统的保护作用。在氧化-LDL诱导的HUVECs模型中，0.02、0.1、0.5、1、2、3、4、5、6 μg/mL的crocin-1和crocin-2'可提高细胞活力达80%。同时，浓度在 25-100 μg/mL 的稀有鳄鱼毒素可使胆固醇诱导的斑马鱼幼体的脂质积累减少 30%。此外，研究人员还探讨了稀有黄腐素对血栓形成的治疗潜力。在体外实验中，浓度为 0.02、0.05、0.2、0.5、1、2、5、10 μg/mL 的稀有鳄鱼素-1 和鳄鱼素-2'可保护人脐静脉内皮细胞（HUVECs）免受脂多糖诱导的氧化应激和炎症的影响。体内研究表明，25、50、100、150、200 和 300 μg/mL 浓度的稀有鳄鱼素对花生四烯酸（AA）诱导的斑马鱼具有显著的抗血栓作用，300 μg/mL 剂量几乎不存在导致斑马鱼畸形的潜在风险。简而言之，稀有鳄鱼毒素具有抗炎、抗氧化和抗凝血的特性，因此被视为治疗心血管疾病的潜在有效候选药物。

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引用次数: 0

Natural compounds for endometriosis and related chronic pelvic pain: A review 治疗子宫内膜异位症和相关慢性盆腔疼痛的天然化合物：综述

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-10-28 DOI: 10.1016/j.fitote.2024.106277

Agostino Zaurito , Irsida Mehmeti , Francesco Limongelli , Roberta Zupo , Alessandro Annunziato , Sergio Fontana , Roberta Tardugno

Endometriosis is a chronic gynecological disorder characterized by significant chronic pelvic pain (CPP) and infertility, adversely affecting the quality of life for many women worldwide.

This review aims to synthesize recent findings on natural bioactive compounds derived from various plant sources that exhibit beneficial effects in the management of endometriosis and related CPP.

A thorough search of databases, including PubMed, Scopus, and Google Scholar, was conducted to identify studies evaluating the efficacy of natural compounds on endometriosis and related CPP. In alphabetical order, curcumins, ginsenosides, polyphenols and other secondary metabolites showed promising effects on oxidative stress, inflammation, and pain modulation associated with endometriosis acting on multiple pathways. Most of the selected articles were in vitro and in vivo studies in animal models, with a limited number of clinical trials.

The reported natural compounds according to the highlighted multiple bioactivities, might be valuable complementary alternatives as supplements, nutraceuticals, or in advanced personalized nutrition. Further clinical investigations are needed to comprehensively evaluate their therapeutic potential, safety, efficacy and to establish effective treatment protocols.

子宫内膜异位症是一种慢性妇科疾病，以严重的慢性盆腔疼痛（CPP）和不孕症为特征，对全球许多妇女的生活质量造成了不利影响。本综述旨在综合从各种植物中提取的天然生物活性化合物的最新研究成果，这些化合物在治疗子宫内膜异位症和相关的 CPP 方面表现出了有益的作用。我们对包括 PubMed、Scopus 和 Google Scholar 在内的数据库进行了全面搜索，以确定评估天然化合物对子宫内膜异位症和相关 CPP 的功效的研究。按字母顺序排列，姜黄素、人参皂苷、多酚和其他次生代谢物对与子宫内膜异位症相关的氧化应激、炎症和疼痛调节有良好的作用。所选文章大多是动物模型的体外和体内研究，临床试验数量有限。根据所强调的多种生物活性，所报道的天然化合物可能是有价值的补充剂、营养保健品或高级个性化营养品。需要进一步开展临床研究，以全面评估其治疗潜力、安全性和有效性，并制定有效的治疗方案。

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引用次数: 0

Exploration of potential mechanism of Sanhua Jiangzhi granules for the treatment of hyperlipidemia based on network pharmacology and experimental verification 基于网络药理学和实验验证的三花姜枝颗粒治疗高脂血症的潜在机制探索

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-10-24 DOI: 10.1016/j.fitote.2024.106271

Junfei Wei , Qian Lv , Fei Luan , Xiaofei Zhang , Dongyan Guo , Bingtao Zhai , Shucun Chen , Junbo Zou , Yajun Shi

Sanhua Jiangzhi Granules (SJG) is a traditional Chinese patent medicine known for regulating lipid metabolism. In this study, we utilized UPLC-TOF-MS to analyze the components of SJG and, in conjunction with network pharmacology, identified 125 core chemical constituents. These components were individually queried and intersected with targets related to hyperlipidemia, resulting in the identification of 312 core targets. KEGG and GO analyses suggested that the mechanism of SJG in treating hyperlipidemia may primarily involve the PPAR signaling pathway. To further validate the efficacy and underlying signaling mechanisms of SJG, we conducted experiments using 60 rats. The results indicated that SJG significantly reduced body weight, lowered serum levels of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), while increasing high-density lipoprotein cholesterol (HDLC) levels. Enzyme-linked immunosorbent assay (ELISA) results demonstrated that SJG decreased hepatic TC and TG levels and mitigated lipid accumulation in the liver. Hematoxylin and eosin (HE) staining indicated that SJG improved liver pathological morphology and reduced the risk of fatty liver disease. Western blot analyses showed that treatment with SJG down-regulated the expression of stearoyl-CoA desaturase (SCD), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), phospholipid transfer protein (PLTP), and fatty acid-binding protein 1 (FABP1), while up-regulating the expression of cholesterol 7α-hydroxylase (CYP7A1), carnitine palmitoyltransferase 1 (CPT-1), and PPARα by activating the PPAR signaling pathway. In conclusion, this study demonstrated that SJG activates the PPAR signaling pathway, leading to decreased body weight, lowered blood lipid levels, reduced hepatic TC and TG, and improved liver pathology in rats.

三花姜芝颗粒（SJG）是一种以调节脂质代谢而闻名的传统中成药。在这项研究中，我们利用 UPLC-TOF-MS 分析了三花姜颗粒的成分，并结合网络药理学，确定了 125 种核心化学成分。我们对这些成分进行了单独查询，并将其与高脂血症相关靶点进行交叉，最终确定了 312 个核心靶点。KEGG 和 GO 分析表明，澳捷治疗高脂血症的机制可能主要涉及 PPAR 信号通路。为了进一步验证 SJG 的疗效及其信号转导机制，我们用 60 只大鼠进行了实验。结果表明，SJG 能显著减轻体重，降低血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）水平，同时提高高密度脂蛋白胆固醇（HDLC）水平。酶联免疫吸附试验（ELISA）结果表明，SJG 降低了肝脏 TC 和 TG 水平，减轻了肝脏中的脂质积累。血红素和伊红（HE）染色表明，SJG 改善了肝脏病理形态，降低了脂肪肝的风险。Western blot 分析表明，使用圣荷西治疗可下调硬脂酰-CoA 去饱和酶（SCD）、3-羟基-3-甲基戊二酰-CoA 还原酶（HMGCR）、磷脂转移蛋白（PLTP）和脂肪酸结合蛋白 1（PLTP）的表达、和脂肪酸结合蛋白 1（FABP1）的表达，同时通过激活 PPAR 信号通路上调胆固醇 7α- 羟化酶（CYP7A1）、肉碱棕榈酰基转移酶 1（CPT-1）和 PPARα 的表达。总之，本研究证明了上海交大可激活 PPAR 信号通路，从而减轻大鼠体重、降低血脂水平、减少肝脏 TC 和 TG，并改善肝脏病理学。

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引用次数: 0

Two new flavonoids from the leaves of Garcinia smeathmannii, in vitro and in silico anti-inflammatory potentials 辣木叶中的两种新黄酮类化合物的体外和体内抗炎潜力。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2024-10-24 DOI: 10.1016/j.fitote.2024.106273

Moïse Sirlam , Peron Bosco Leutcha , Guy Raphael Sado Nouemsi , Humaira Zafar , Hycienth Fung Tegha , Denis Kehdinga Sema , Virginie Flaure Tsague Tankeu , Yves Oscar Nganso Ditchou , Madan Poka , Patrick Hulisani Demana , Atia-tul-Wahab , Muhammad Iqbal Choudhry , Xavier Siwe Noundou , Alain Meli Lannang

Garcinia smeathmannii is a well-known plant for its uses in the effective treatment of intestinal parasites, skin eruptions and skin burns. The dichloromethane-methanol (2:3) crude extract of the leaves of G. smeathmannii led to the isolation and characterization of twenty compounds (1−20) using chromatographic and spectroscopic techniques. Extracts and compounds were screened in vitro for their anti-inflammatory (ROS), antiglycation and antileishmanial (L. tropica) activities. Compounds were also screened for their in silico anti-inflammatory activities using Maestro 4.2.1 software with the co-crystal complex structures of the ovine oCOX-1: meloxicam (PDB Id: 4O1Z) and murine mCOX-2: meloxicam (PDB Id: 4M11) proteins. An unprecedented flavonol (1) and a flavone dimer (2) together with eighteen known compounds (3−20) were characterized. All the tested samples in vitro revealed no antiglycation and antileishmanial activities. Beside, extracts revealed moderate anti-inflammatory activities (IC₅₀ ranging from 24.1 ± 2.0 to 34.7 ± 0.8 μg/mL). Only compound (13) revealed an anti-inflammatory activity which was 9.33 times more active than the reference (Ibuprofen, IC₅₀ = 11.2 ± 1.9 μg/mL) with IC₅₀ of 1.2 ± 0.0 μg/mL. Compounds (2–9, 11–13 and 19–20) were docked and the docking scores were ranging from −10.178 to −6.119 (kcal/mol) which were in agreement with the experimental anti-inflammatory activity. These results are in agreement with the traditional uses of the leave of G. smeathmannii as cataplasm for skin eruption and as analgesic agent.

胡枝子是一种著名的植物，可用于有效治疗肠道寄生虫、皮肤糜烂和皮肤烧伤。利用色谱和光谱技术，通过二氯甲烷-甲醇（2:3）的粗萃取，分离并鉴定了二十种化合物（1-20）。对提取物和化合物进行了体外抗炎（ROS）、抗糖化和抗利什曼病（L. tropica）活性筛选。此外，还使用 Maestro 4.2.1 软件，结合绵羊 oCOX-1：美洛昔康（PDB Id：4O1Z）和鼠 mCOX-2：美洛昔康（PDB Id：4M11）蛋白的共晶体复合结构，对化合物的抗炎活性进行了硅学筛选。对一种前所未有的黄酮醇（1）和一种黄酮二聚体（2）以及 18 种已知化合物（3-20）进行了表征。所有体外测试样品均未显示抗糖化和抗利什曼活性。此外，提取物显示出中等程度的抗炎活性（IC50 范围为 24.1 ± 2.0 至 34.7 ± 0.8 μg/mL）。只有化合物（13）显示出抗炎活性，其活性是参照物（布洛芬，IC50 = 11.2 ± 1.9 μg/mL）的 9.33 倍，IC50 为 1.2 ± 0.0 μg/mL。对化合物（2-9、11-13 和 19-20）进行了对接，对接得分在 -10.178 至 -6.119 (kcal/mol) 之间，与实验抗炎活性一致。这些结果与传统上将 G. smeathmannii 的叶子用作治疗皮肤糜烂和镇痛剂的方法一致。

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