Fitoterapia最新文献_第7页

Multi-faceted bioactivity of three unexplored Turkish plants: A comparative phytochemical and pharmacological profiling 三种未开发的土耳其植物的多方面生物活性：比较植物化学和药理学分析。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-25 DOI: 10.1016/j.fitote.2025.107066

Nilofar , Gunes Ak , Enver Saka , Gokhan Zengin , Cziaky Zoltan , Josef Jeko , Annalisa Chiavaroli , Simonetta Cristina Di Simone , Mariachiara Gabriele , Maria Loreta Libero , Giustino Orlando , Luigi Menghini , Claudio Ferrante , Jasmina Glamočlija , Mladen Rajaković , Evren Yildiztugay , Meng-Yao Li

Since ancient times, plants have been used as a valuable source for treating serious diseases. Three different plants, namely Aubrieta pinardii, Johrenia dichotoma, and Malabaila dasyantha were used and extracted using the extraction solvents ethyl acetate (EA), methanol (MeOH), and water. Antioxidant activity was determined using several in vitro assays. Subsequently, the enzyme inhibition activities of the extracts were evaluated against cholinesterases (AChE and BChE), tyrosinase, amylase, and glucosidase. The antibacterial and antifungal effects of the extracts were assessed. Methanol extracts of A. pinardii, J. dichotoma, and M. dasyantha yielded the highest total flavonoids (TFC) compared to EA and water extracts. The analysis using HPLC-ESI-Q-TOF-MS identified various classes of phytochemicals within the species examined. Aubrieta pinardii extracts were abundant in glucosinolates, whereas J. dichotoma and M. dasyantha extracts were characterized by the presence of coumarins and furanocoumarins. The EA extracts exhibited greater inhibition of microorganism growth compared with MeOH and water extracts. Notably, J. dichotoma showed similar or superior antifungal activity against A. niger (MIC/MFC 0.25/0.50 mg/mL) and M. dasyantha demonstrated comparable efficacy against T. viride (MIC/MFC 0.50/1.00 mg/mL) and P. verrucosum var. cyclopium (MIC/MFC 0.125/0.25 mg/mL) when benchmarked against the reference drug ketoconazole. These results provide valuable information about A. pinardii, J. dichotoma, and M. dasyantha chemical profiles and underline the importance of selecting appropriate solvents for the extraction of metabolites from plant matrix for potential applications in the treatment of various diseases.

自古以来，植物就被用作治疗严重疾病的宝贵资源。采用三种不同的植物，即红毛赤杨（Aubrieta pinardii）、二叉菊（Johrenia dichotoma）和马拉贝拉（Malabaila dasyantha），分别用乙酸乙酯（EA）、甲醇（MeOH）和水进行提取。采用几种体外测定法测定其抗氧化活性。随后，研究了提取物对胆碱酯酶（AChE和BChE）、酪氨酸酶、淀粉酶和葡萄糖苷酶的抑制活性。评价了各提取物的抗菌和抗真菌作用。与EA和水提取物相比，pinardii， J. dichotoma和M. dasyantha甲醇提取物的总黄酮（TFC）含量最高。HPLC-ESI-Q-TOF-MS分析鉴定出不同种类的植物化学物质。红毛紫苏提取物中硫苷含量较高，而双花紫苏和大花紫苏提取物中主要含有香豆素和呋喃香豆素。与MeOH和水提取物相比，EA提取物对微生物生长的抑制作用更大。值得注意的是，当与对照药物酮康唑作对照时，J. dichotoma对黑曲霉（MIC/MFC 0.25/0.50 mg/mL）和M. dasyantha对T. viride （MIC/MFC 0.50/1.00 mg/mL）和P. verrucosum var. cyclopium （MIC/MFC 0.125/0.25 mg/mL）表现出相似或更好的抗真菌活性。这些结果为pinardii， J. dichotoma和M. dasyantha的化学特征提供了有价值的信息，并强调了选择合适的溶剂从植物基质中提取代谢物的重要性，以潜在地应用于治疗各种疾病。

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引用次数: 0

Novel diterpenoid glycosides from Rubus chingii Hu induce apoptosis in BPH-1 cells through fas/caspase-8 pathway activation 新二萜苷通过fas/caspase-8通路激活诱导BPH-1细胞凋亡

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-25 DOI: 10.1016/j.fitote.2025.107069

Jing Wang , Xue Zhang , Lu Chen , Jun Du , Xiaohong Wu , Liang Chen , Yingchun Wu , YiMing Li

Rubus chingii Hu, a food material with medicinal value, has been used to treat urinary system diseases, such as prostatitis, benign prostate hyperplasia (BPH) and lower urinary tract symptoms (LUTS). However, there is no research on the efficacy and mechanism of compounds of R. chingii. in treating BPH. In this study, 10 previously unreported ent-pimarane-type (rubuschinoside A–B, 1–2), ent-isopimarane-type (rubuschinoside CD, 3–4) and ent-labdane-type (goshonoside J16–21, 5–10) diterpene glycosides were isolated from the dried fruits of R. chingii. Their structures were elucidated and confirmed using high-resolution electrospray ionization mass spectrometry (HRESIMS), nuclear magnetic resonance spectroscopy (NMR), single crystal X-ray diffraction and electron capture detector (ECD). All compounds were tested for their apoptosis-inducing activity in BPH cells, and compounds 1, 3, 4 and 8 exhibited significant pro-apoptotic effects. Further apoptosis mechanistic studies on compound 8 revealed that it could increase the expression of Fas, FADD, caspase 3 and caspase 8 mRNA expression levels at 50 μM. Collectively, this study suggested that diterpenoid glycosides from R. Chingii may induce apoptosis in hyperplastic prostate cells through activation of the Fas/caspase-8 signaling cascade.

青藤胡是一种具有药用价值的食品材料，已被用于治疗泌尿系统疾病，如前列腺炎、良性前列腺增生（BPH）和下尿路症状（LUTS）。然而，对青霉中化合物的药效及作用机制尚无研究。治疗前列腺增生本研究从红桃干果中分离得到10个未见报道的对海马烷型（苦楝子苷A-B， 1-2）、对异海马烷型（苦楝子苷CD， 3-4）和对唇丹型（槐子苷j16 - 21,5 - 10）二萜苷。利用高分辨率电喷雾电离质谱（hremms）、核磁共振谱（NMR）、单晶x射线衍射和电子捕获检测器（ECD）对它们的结构进行了分析和证实。结果表明，化合物1、3、4和8对BPH细胞具有明显的促凋亡作用。进一步的凋亡机制研究表明，化合物8在50 μM时可提高Fas、FADD、caspase 3和caspase 8 mRNA的表达水平。综上所述，本研究提示，红姜中的二萜苷可能通过激活Fas/caspase-8信号级联来诱导前列腺增生细胞凋亡。

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引用次数: 0

Formation of series reactive dialdehyde intermediates by metabolism of monotropein 单蛋白代谢形成系列反应性双醛中间体

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-25 DOI: 10.1016/j.fitote.2025.107072

Xiaoli Li , Zejun Liu , Qiuyi Jing , Hong Pan , Fuguo Shi

Monotropein, an iridoid glycoside, is the principal bioactive constituent with the highest amount isolated from Morindae officinalis radix, which has been reported to have potent pharmacological properties. Monotropein contains a hemiacetal structure. The hemiacetal may be converted into a reactive dialdehyde intermediate and covalently bind to the primary amine. Therefore, we speculated that the metabolites of monotropein with hemiacetal can generate dialdehyde intermediates, a class of highly reactive metabolites that enable covalent modification of proteins. This study characterized the metabolic profiling of monotropein, especially the unstable dialdehyde intermediates, using UPLC-Q/Orbitrap MS. A total of eighteen stable metabolites were characterized in monotropein-treated rats. The main metabolic pathways were dehydration, deglycosylation, and decarboxylation. It is noteworthy that six unstable dialdehyde intermediates of monotropein were characterized for the first time. They were the hemiacetal ring-opening products of M4 (monotropein aglycone), M5 (decarboxylated aglycone), M6 (methylated aglycone), M7 (dehydrated aglycone), M8 (hydrogenated aglycone), and M9 (dehydroxymethyl aglycone). The dialdehyde intermediates were identified by their stable dihydropyridine conjugates with amino acids. Five dialdehyde intermediates enabled covalent modification of proteins in the intestine. In conclusion, eighteen stable metabolites and six reactive dialdehyde intermediates were characterized in this study, which contributes to understanding the pharmacological effects of monotropein.

单tropein是一种环烯醚萜苷，是从桑台中分离出的含量最高的主要生物活性成分，据报道具有有效的药理作用。单肌蛋白含有半缩醛结构。半缩醛可转化为活性双醛中间体并与伯胺共价结合。因此，我们推测单肌蛋白与半缩醛的代谢物可以产生双醛中间体，这是一类高活性的代谢物，可以对蛋白质进行共价修饰。本研究利用UPLC-Q/Orbitrap ms表征了单肌蛋白的代谢谱，特别是不稳定的双醛中间体。在单肌蛋白处理的大鼠中，共有18种稳定的代谢物被表征。主要代谢途径为脱水、去糖基化和脱羧。值得注意的是，本文首次对六种不稳定的单蛋白双醛中间体进行了表征。它们分别是M4（单斜蛋白苷元）、M5（脱羧苷元）、M6（甲基化苷元）、M7（脱水苷元）、M8（氢化苷元）和M9（去羟甲基苷元）的半缩醛开环产物。双醛中间体通过与氨基酸稳定的二氢吡啶偶联物进行鉴定。五种双醛中间体使肠道中蛋白质的共价修饰成为可能。综上所述，本研究鉴定了18种稳定代谢产物和6种活性双醛中间体，有助于了解单肌蛋白的药理作用。

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引用次数: 0

Terpenoids from the whole plants of Chloranthus holostegius 全株青兰的萜类化合物

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-24 DOI: 10.1016/j.fitote.2025.107065

Huan Yan , Yong-Sheng Zhou , Long-Gao Xiao , Wei Ni , Yupeng Geng , Hai-Yang Liu

The phytochemical reinvestigation on the whole plants of Chloranthus holostegius led to the isolation of five undescribed sesquiterpenes (named chloranholosins U-Y, 1–5), including two lindenane-type sesquiterpenes, two acorane-type sesquiterpenes, and one elemane-type sesquiterpene, together with 30 known compounds. Their structures and absolute configurations were elucidated by a comprehensive method including the spectroscopic data, and electronic circular dichroism (ECD) calculations. Chloranholosin U (1) was elucidated as a rare lindenane–dinormonotepenoid hybrid with an oxa-difuranofurone moiety. Furthermore, all new isolates and 18 known compounds were evaluated for their inhibitory activity against LPS-induced nitric oxide (NO) production in RAW 264.7 macrophage cells, while their cytotoxic activities were also assessed against five human tumor cell lines. Though these compounds did not show inhibitory activity for NO production, compounds 16, 34, and 35 displayed moderate cytotoxicity. Compound 16 showed cytotoxicity against HL-60, HepG2, MDA-MB-231, and SW480 (IC₅₀ 10.98–36.48 μM), compounds 34 and 35 were active on HL-60 (IC₅₀ 18.98 and 14.89 μM), and 34 also inhibited A549 (IC₅₀ 24.32 μM).

通过对全株Chloranthus holostegius的植物化学再研究，分离到5种未被描述的倍半萜（命名为chloranholosins U-Y， 1-5），包括2种椴树烷型倍半萜、2种橡树烷型倍半萜和1种烯烯型倍半萜，以及30种已知化合物。用光谱数据和电子圆二色性（ECD）计算等综合方法对它们的结构和绝对构型进行了分析。Chloranholosin U(1)是一种罕见的具有氧-二呋喃呋喃基团的lindenane - dinormontepenoid杂合体。此外，所有新分离物和18种已知化合物对lps诱导的RAW 264.7巨噬细胞产生一氧化氮（NO）的抑制活性进行了评估，同时对5种人类肿瘤细胞系进行了细胞毒活性评估。虽然这些化合物对NO的产生没有抑制活性，但化合物16、34和35表现出中等的细胞毒性。化合物16对HL-60、HepG2、MDA-MB-231和SW480的IC50值为10.98 ~ 36.48 μM，化合物34和35对HL-60的IC50值为18.98和14.89 μM，化合物34对A549的IC50值为24.32 μM。

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引用次数: 0

Anti-inflammatory triterpenoids from dammar resin of Vatica mangachapoi 枫香树脂中抗炎三萜。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-23 DOI: 10.1016/j.fitote.2025.107056

Rui-Jie Feng , De-Li Chen , Jian-He Wei , Hui Meng , Yun Yang

Five previously undescribed triterpenoids (1–5), and four triterpenoids (6–9) which had been synthesized previously but had not been isolated from a natural source, along with twenty-three known triterpenoids (10−32) were isolated from the methanol extract of dammar resin of Vatica mangachapoi. HRESIMS, NMR, UV, and ECD spectroscopy were employed to determine the structures of these triterpenoids. Compounds 2, 6, 22, and 29 significantly reduced LPS-induced TNF-α levels in RAW264.7 cells, while compounds 3, 13, 16, and 17 reduced IL-6 levels at a concentration of 10 μM. These results suggest that these compounds exhibit potential anti-inflammatory activity.

从Vatica mangachapoi达玛树脂的甲醇提取物中分离得到5个未被描述的三萜（1-5）和4个未从天然来源中分离得到的三萜（6-9），以及23个已知的三萜（10-32）。采用HRESIMS、NMR、UV、ECD等方法对这些三萜进行了结构表征。化合物2、6、22和29显著降低lps诱导的RAW264.7细胞中TNF-α水平，而化合物3、13、16和17在浓度为10 μM时显著降低IL-6水平。这些结果表明这些化合物具有潜在的抗炎活性。

引用次数: 0

Involvement of the NO-sGC/cGMP signaling pathway in the vasodilator and hypotensive effects of the ethanol-soluble fraction from Citrullus lanatus (Thunb.) Matsum. & Nakai seeds in rats NO-sGC/cGMP信号通路参与甜瓜醇溶部分的血管扩张和降压作用（Thunb.）Matsum。& Nakai种子在老鼠体内。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-23 DOI: 10.1016/j.fitote.2025.107041

Aline Aparecida Macedo Marques , Karyne Garcia Tafarelo Moreno , Luciane Barbosa Pessoa , Luana Ale Bertoncello Pael , Katiana Simões Kittelson , Ariany Carvalho dos Santos , Gabriela Pereira da Silva , Bianca Viana Silva , Maria Luiza Fidelis da Silva , Luana Bonifácio Sanches , Denise Brentan da Silva , Arquimedes Gasparotto Junior

The therapeutic management of hypertension involves both pharmacological and non-pharmacological interventions. However, control remains inadequate despite effective treatments, often due to poor adherence. In this context, herbal medicines offer a complementary approach to conventional therapy. Citrullus lanatus (watermelon) seeds, frequently discarded as agricultural byproducts, represent a promising source of bioactive compounds. This study evaluated the cardiorenal properties of three extracts obtained from dried C. lanatus seeds, including an ethanol-soluble fraction from an aqueous extract (ESCL), a hydroethanolic extract, and an aqueous extract obtained by turbolysis. These extracts were chemically characterized using liquid chromatography coupled with diode array detection and mass spectrometry. Diuretic and hypotensive activities were assessed in vivo in normotensive female Wistar rats, while vasodilatory mechanisms were investigated ex vivo in isolated mesenteric vascular beds. Among the preparations screened, only the ESCL fraction displayed significant cardiorenal activity, reducing blood pressure and peripheral vascular resistance. Mechanistic investigation revealed that this vascular response is mediated via the nitric oxide/soluble guanylate cyclase/cyclic guanosine monophosphate signaling cascade. These findings highlight the pharmacological potential of ESCL as a candidate for the development of novel hypotensive agents.

高血压的治疗管理包括药物和非药物干预。然而，尽管有有效的治疗，但控制仍然不足，这往往是由于依从性差。在这种情况下，草药为传统疗法提供了一种补充方法。作为农业副产品经常被丢弃的西瓜种子是一种很有前途的生物活性化合物来源。本研究评估了三种从干羊草种子中提取的提取物的心肾特性，包括水提取物（ESCL）的乙醇溶性部分，氢乙醇提取物和通过涡轮分解获得的水提取物。利用液相色谱、二极管阵列检测和质谱法对这些提取物进行了化学表征。在正常血压的雌性Wistar大鼠体内评估利尿和降压活性，并在离体肠系膜血管床中研究血管舒张机制。在筛选的制剂中，只有ESCL组分显示出显著的心肾活性，降低血压和外周血管阻力。机制研究表明，这种血管反应是通过一氧化氮/可溶性鸟苷环化酶/环鸟苷单磷酸信号级联介导的。这些发现突出了ESCL作为开发新型降压药物的候选药物的药理学潜力。

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引用次数: 0

Antioxidant active ingredients from the seeds of Dimocarpus longan Lour 龙眼种子抗氧化活性成分的研究。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-23 DOI: 10.1016/j.fitote.2025.107043

Bin Liu , Yang Xu , Lingxiao Ren , Xiaofen Liu , Lixia Chen , Hua Li

The seeds of Dimocarpus longan Lour. are rich in bioactive compounds, notably polyphenols, flavonoids, and sesquiterpenoids, which have demonstrated antioxidant, antitumor, and antifungal activities. Phytochemical investigation of the seeds led to the isolation of thirteen compounds (1−13), comprising six previously undescribed structures (1–6) and seven known analogues (7–13). The structures of the new compounds were elucidated through comprehensive spectroscopic analysis, including 1D/2D NMR, HR-ESI-MS, UV, ECD, and optical rotation. All isolated compounds were evaluated for their protective effects against hydrogen peroxide-induced oxidative stress in BV-2 microglial cells. Among them, compounds 2, 3, 7, 11, and 12 exhibited significant antioxidant activity. These findings underscore the potential of Dimocarpus longan seeds as a valuable source of bioactive agents for medicinal and functional food applications.

龙眼树的种子。富含生物活性化合物，特别是多酚、类黄酮和倍半萜类化合物，具有抗氧化、抗肿瘤和抗真菌活性。对种子的植物化学研究分离出13种化合物（1-13），包括6种以前未描述的结构（1-6）和7种已知的类似物（7-13）。通过1D/2D NMR、HR-ESI-MS、UV、ECD、旋光度等综合光谱分析对新化合物的结构进行了鉴定。所有分离的化合物对BV-2小胶质细胞过氧化氢诱导的氧化应激的保护作用进行了评估。其中化合物2、3、7、11和12表现出显著的抗氧化活性。这些发现强调了龙眼种子作为药用和功能性食品生物活性物质的宝贵来源的潜力。

引用次数: 0

Quinoline and para-aminobenzoic acid derivatives with anti-osteoclastic activity from a marine mesophotic zone Demosponge sponge-associated fungus Xylaria sp. NBUF245 海洋间孔区海绵相关真菌Xylaria sp. NBUF245的喹啉和对氨基苯甲酸衍生物具有抗破骨活性。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-23 DOI: 10.1016/j.fitote.2025.107046

Yu-Ying Liu , Chan-Ting Lai , Salman Khan , Qian-Jing Zheng , Rong Zhang , Wei-Yi Wang , Bin Wu , Shan He , Li Yang , Ting-Ting Wang

One undescribed quinoline derivative, xylaminoquin A (1), one new p-aminobenzoic acid derivative, xylamidate B (2), and methyl 4-(butanoylamino)-3-hydroxybenzoate, reported for the first time as a natural product and named xylamidate C (3), together with known alkaloids and diketopiperazines, methyl 4-acetylamino-3-hydroxybenzoate (4), methyl 4-[(1-oxobutyl)amino]benzoate (5), pseurotin A (6), azaspirofuran A (7), cyclo(l-Tyr-l-Leu) (8), and cyclo-(l-Tyr-l-Val) (9) were isolated from Xylaria sp. NBUF245, a fungus associated with a Demosponge sp. collected from the marine mesophotic zone. Structurally, compound 1 is the first example of 3,7-dissubstitented quinoline derivative isolated from fungi. The structures of all compounds were elucidated by combined analysis of spectroscopic data and X-ray crystallography. Bioassay results showed that compound 5 effectively prevented prednisolone-induced bone loss in zebrafish at a concentration of 30 μM, suggesting potential osteoprotective activity.

一种未被描述的喹啉衍生物xylaminoquin A(1)，一种新的对氨基苯甲酸衍生物xylamidate B(2)和甲基4-（丁酰氨基）-3-羟基苯甲酸甲酯，首次作为天然产物报道并命名为xylamidate C(3)，以及已知的生物碱和二酮哌嗪，甲基4-乙酰氨基-3-羟基苯甲酸甲酯(4)，甲基4-[（1-氧丁基）氨基]苯甲酸甲酯(5)，假黄素A (6), azaspirofuran A(7)，环（l-Tyr-l-Leu） (8)，和cyclo-(l-Tyr-l-Val)(9)分别从海洋中孔带采集的与Demosponge sp.相关的Xylaria sp. NBUF245真菌中分离得到。结构上，化合物1是首次从真菌中分离得到的3,7-非取代喹啉衍生物。所有化合物的结构都通过光谱数据和x射线晶体学的结合分析得到了证实。生物测定结果表明，化合物5在浓度为30 μM时可有效预防强的松龙诱导的斑马鱼骨质流失，提示其具有潜在的骨保护作用。

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引用次数: 0

Anti-inflammatory perylenequinones from the endolichenic fungus Phialocephala fortinii triggered by a histone deacetylase inhibitor 由组蛋白去乙酰化酶抑制剂引发的内源性真菌富尔替尼的抗炎二烯丙二烯酮。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-22 DOI: 10.1016/j.fitote.2025.107051

Ke Xu , Xiao-Bin Li , Yu-Liang Xu , Fei Xie , Hong-Xiang Lou

Inclusion of the histone deacetylase (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) into the culture medium of the endolichenic fungus Phialocephala fortinii resulted in the separation of three new perylenequinones phialocephalarins E-G (1–3) and enhanced the production of the known compounds phialocephalarins A-B (4–5). A comprehensive spectral analysis combined with electron circular dichroism (ECD) data has elucidated their structures. Phialocephalarin E (1) displayed anti-inflammatory activity with the maximum inhibition rate of 57.3 % ± 7.3 % (50 μM). Further molecular docking experiment revealed the affinity degree of compound 1 to the prostaglandin E2 receptor 4 (EP4R) and the molecular dynamics (MD) simulation results showed compound 1-EP4R complex had relatively good binding stability.

将组蛋白去乙酰化酶（HDAC）抑制剂亚eroylanilide羟肟酸（SAHA）加入到内生真菌phalocephalala fortinii的培养基中，可分离出三种新的过烯丙二烯类化合物phalocephalarins E-G(1-3)，并促进了已知化合物phalocephalarins A-B的生成（4-5）。综合光谱分析结合电子圆二色性（ECD）数据阐明了它们的结构。phalocephalarin E(1)表现出抗炎活性，最大抑制率为57.3% % ± 7.3 %（50 μM）。进一步的分子对接实验揭示了化合物1与前列腺素E2受体4 （EP4R）的亲和程度，分子动力学（MD）模拟结果显示化合物1-EP4R复合物具有较好的结合稳定性。

引用次数: 0

Modulation of immune cell differentiation by Prunella vulgaris L. essential oil in pulmonary sarcoidosis treatment 夏枯草精油对肺结节病免疫细胞分化的调节作用。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia

Pub Date : 2025-12-22 DOI: 10.1016/j.fitote.2025.107049

Zhiguo Mao , Xingyi Yang , Ying Liu , Yu Huan , Yixue Gao , Jinying Zhang , Xiangke Lin , Shuo Tian , Yagang Song , Mingsan Miao

Background

Prunella vulgaris L. is a traditional Chinese medicine known for its ability to disperse nodules and reduce swelling. However, research on the effect of the volatile oil from Prunella vulgaris L. on pulmonary nodulosis remains limited.

Aim of the study

To investigate whether essential oil from Prunella vulgaris L. (PVEO) improves pulmonary function in PS mice by inhibiting the inflammation mediated by the TLR4/MyD88/NF-κB pathway.

Methods

Network pharmacology was used to explore how PVEO might treat PS. GC–MS identified PVEO's chemical components. A PS mouse model was created using Propionibacterium acnes. ELISA measured inflammatory factors in the BALF and blood of these mice. Flow cytometry analyzed CD4⁺ T cell differentiation into TH1 and TH17 cells. Immunofluorescence assessed macrophage differentiation into M1 and M2 phenotypes and the expression of TLR4, MyD88, and NF-κB. PCR and WB were conducted to detect the expression of mRNAs and proteins related to the TLR4/MyD88/NF-κB pathway.

Results

Network pharmacology predictions indicated that the improvement of pulmonary function by PVEO in PS mice was associated with the TLR4/MyD88/NF-κB signaling pathway. PCR and western blot (WB) analyses demonstrated that PVEO inhibited the expression of relevant mRNAs and proteins within the TLR4/MyD88/NF-κB pathway. Immunofluorescence and flow cytometry assays suggested that PVEO suppressed the differentiation of M1 macrophages, TH1, and TH17 cells in lung tissues of PS mice.

Conclusion

PVEO exerts anti-PS effects by inhibiting the inflammation mediated by the TLR4/MyD88/NF-κB pathway in macrophages.

背景：夏枯草（Prunella vulgaris L.）是一种以散瘤消肿著称的中药。然而，关于夏枯草挥发油对肺结节病的作用的研究还很有限。研究目的：探讨枯草精油（PVEO）是否通过抑制TLR4/MyD88/NF-κB通路介导的炎症，改善PS小鼠肺功能。方法：采用网络药理学方法探讨PVEO对PS的治疗作用，GC-MS鉴定PVEO的化学成分。采用痤疮丙酸杆菌建立小鼠PS模型。ELISA法测定这些小鼠的BALF和血液中的炎症因子。流式细胞术分析CD4+ T细胞向TH1和TH17细胞分化的情况。免疫荧光检测巨噬细胞向M1和M2表型分化及TLR4、MyD88和NF-κB的表达。采用PCR和WB检测TLR4/MyD88/NF-κB通路相关mrna和蛋白的表达情况。结果：网络药理学预测提示PVEO对PS小鼠肺功能的改善与TLR4/MyD88/NF-κB信号通路有关。PCR和western blot （WB）分析表明，PVEO抑制TLR4/MyD88/NF-κB通路相关mrna和蛋白的表达。免疫荧光和流式细胞术检测显示，PVEO可抑制PS小鼠肺组织中M1巨噬细胞、TH1和TH17细胞的分化。结论：PVEO通过抑制巨噬细胞TLR4/MyD88/NF-κB通路介导的炎症，发挥抗ps作用。

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引用次数: 0