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PO-243 Correlation between exercise performance and muscle electrical activity in Exercise-induced Fatigue Rats 运动性疲劳大鼠运动表现与肌电活动的相关性研究
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I5.10913
Haojie Huang, Mengjiao Chen, L. Hou, Xiaoli Liu, D. Qiao
Objective Assess the muscle contraction ability of rats before and after exercise fatigue quantitatively, and analyze the correlation between exercise performance and muscle electrical activity. This study intends to provide a theoretical principle for exercise fatigue. Methods 7 healthy adult male SD rats (300~350g) were used and trained for adaptive treadmill by running on the treadmill for 1 week and holding on a vertical rectangular railing (20×10cm, 10 iron bars) until it's mastered. After this training, we used rat grasping force tester( BioSEB GS3) to measure the maximum grasping force(MGF) of rat's limbs, each rat was carried out 3 times, bout interval is 2 minutes. In addition, the grasping bar time(GBT) was recorded 3 times , bout interval is 30 minutes. We let rats to grasp and hold on a vertical rectangular railing(20×10cm, 10 iron bars), evaluation of rat muscular endurance by grasping bar time(GBT), each rat was carried out 3 times, bout interval is 30 minutes; During the MGF and GBT test, motor unit recruitment and discharge frequency was predicted by measuring the EMG of extensor muscles of the right hindlimb and flexor elbow muscles of the right forelimb by wireless non-invasive miniature surface EMG tester (Italy, BTS FREEEMG), the max Root Mean Square (maxRMS) and Median Frequency (MF) parameter was used to evaluate motor unit recruitment and discharge frequency, respectively. After these, the rats were allowed to have a one-day rest, and then had a load motion program on the treadmill (three levels’ load: the first stage movement speed 8.2 m/min, exercise time 15 min; second stage speed 15 m/min, exercise time 15 min; third stage speed at 20 m/min, exercise to fatigue ) to build the rats EF model by monitoring the acceleration of the rat's sprint with a miniature wireless acceleration sensor (18g). 30 continuous sprint acceleration at the end of running was less than half of initial acceleration and the running posture of the rats changed to prostrate, and remained at the end of the runway for a long time. Later, quantitative correlational data analyses such as mean, Pearson correlation, analysis of one-way ANOVA and paired sample t test were performed in this study. Results (1) The rats’ sprint acceleration of treadmill exercise at the end stage (the final 1/5 of the total time) decreased by 56.9% (P < 0.01) when compared with the early stage (the begining 1/5 of the total time). (2) The MGF and GBT of EF decreased by 68.1% (P < 0.01) and 90.38% (P < 0.01), respectively when compared with the beginning EF; in addition, the EMG maxRMS and MF of hindlimb and forelimb of EF rats had significantly reduced (P < 0.01), and the rats’ MGF/GBT was positively correlated with EMG maxRMS/MF significantly (MGF: forelimb rmaxRMS = 0.901, P < 0.01,rMF = 0.761, P < 0.01; hindlimb rmaxRMS = 0.913, P < 0.01,rMF = 0.783, P < 0.01; GBT: forelimb rmaxRMS = 0.922, P < 0.01,rMF = 0.806, P < 0.01; hindlimb rmaxRMS = 0.908, P < 0.01,rMF = 0.896, P
目的定量评价运动疲劳前后大鼠的肌肉收缩能力,分析运动表现与肌电活动的相关性。本研究旨在为运动性疲劳提供理论依据。方法选用健康成年雄性SD大鼠7只(300~350g),通过在跑步机上跑步1周,并抓住垂直矩形栏杆(20×10cm, 10根铁条),直至熟练掌握,进行适应性跑步训练。训练结束后,采用大鼠抓握力测试仪(BioSEB GS3)测量大鼠四肢的最大抓握力(MGF),每只大鼠进行3次,每次间隔2分钟。同时记录抓棒时间(GBT) 3次,每次间隔30分钟。我们让大鼠抓握垂直矩形栏杆(20×10cm, 10根铁棒),通过抓杆时间(GBT)评价大鼠肌肉耐力,每只大鼠进行3次,每次间隔30分钟;在MGF和GBT试验中,通过无线无创微型表面肌电仪(意大利BTS FREEEMG)测量右后肢伸肌和右前肢屈肘肌的肌电信号预测运动单元的招募和放电频率,分别采用最大均方根(maxRMS)和中位数频率(MF)参数评估运动单元的招募和放电频率。在此之后,大鼠休息一天,然后在跑步机上进行负荷运动程序(三级负荷:第一阶段运动速度8.2 m/min,运动时间15 min;第二阶段速度15m /min,运动时间15min;第三阶段速度为20 m/min,运动至疲劳),利用微型无线加速度传感器(18g)监测大鼠冲刺时的加速度,建立大鼠EF模型。30连续冲刺加速度在跑步结束时小于初始加速度的一半,大鼠的跑步姿势变为俯卧,并长时间停留在跑道终点。随后,本研究进行了均值、Pearson相关、单因素方差分析、配对样本t检验等定量相关数据分析。结果(1)大鼠在跑步机运动结束阶段(最后1/5总时间)的冲刺加速度较运动早期(开始1/5总时间)降低56.9% (P < 0.01)。(2)与初伐期相比,采伐期MGF和GBT分别下降了68.1% (P < 0.01)和90.38% (P < 0.01);此外,EF大鼠后肢和前肢肌电最大均方根值和MF均显著降低(P < 0.01), MGF/GBT与EMG最大均方根值/MF显著正相关(MGF:前肢rmaxRMS = 0.901, P < 0.01,rMF = 0.761, P < 0.01;后肢rmaxRMS = 0.913, P < 0.01,rMF = 0.783, P < 0.01;GBT:前肢rmaxRMS = 0.922, P < 0.01,rMF = 0.806, P < 0.01;后肢rmaxRMS = 0.908, P < 0.01,rMF = 0.896, P < 0.01)。结论运动疲劳使大鼠的肌力、肌肉耐力和肌力明显降低,这可能与前肢屈肌和后肢伸肌肌肉运动单元的补充、节律同步性和放电频率降低有关。
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引用次数: 0
PO-081 Overtraining results in abnormality of renal silt diaphragm in rats with persistent proteinuria PO-081过度训练导致持续性蛋白尿大鼠肾粉膜异常
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I3.11423
Y. Niu, Jianmin Cao, Hai-tao Zhou, H. Cao
Objective Silt diaphragm is the most important and bioactive membrane structure in the filtration barrier of kidney, and the root cause of proteinuria is the structural and functional abnormalities of Silt diaphragm. So far, there is little literature on the changes of silt diaphragm caused by overtraining. This research establishes a model of rats with exercise-induced proteinuria with long-term intensitive treadmill exercise, and it simulates the progressive-load training in the cycle of athletes. Histological and ultrastructural changes of kidney immediately and 24 h after exercise are observed, and it aims to analyze the change law of silt diaphragm during the occurrence of persistent proteinuria. Methods this study selects 36 Sprague-Dawley rats, which are randomly divided into 3 groups: a control group (group C, 12), a group drawn immediately after exercise(group EI, 12), a group drawn 24 h after exercise(group EA, 12). Group C does not train. The rats in group EI and EA train on the treadmill with an increasing load for 6 weeks(10% grade, 6 d/w): in the first week, the rats run for 10 min at 10 m/min. Starting from the second week, the running speed increases by 5m/min/w, and the training time increases by 30min/w. In the last week the rats run to exhaustion if they could not maintain the target intensity. Record the exhausting time of rats, then group EI and group EA are respectively drawn immediately and 24 hours after exercise. Observe the histological changes of  renal glomerulus by optical microscope, and the ultrastructure of silt diaphragm by TEM. Detect urine total protein by BCA, serum and urine creatinine by Jaffe, serum testosterone and corticosterone by radioimmunoassay, serum urea by two-point dynamic method, and the expression of Nephrin by western-blot. Results The rats in group EI and EA gradually lose weight at the first weekend of training, and their weight drop significantly from the third weekend to the end, it shows a significant difference compared with group C(p<0.01). There is no significant difference between the exercise group. Glomerular morphology, group C: The structure of glomerulus is compact, the boundary between vascular sphere and  the wall of capsule is obvious, and the distribution of erythrocytes in vessels is regular; Group EI: The thickness of glomerulus membrane is uneven, the structure of the podocyte is incomplete, part of the foot process is fused, and SD is discontinuous; Group EA: Part of the glomerular endodermis is abnormal, part of the foot process is fused, detached, and unevenly distributed, and SD is discontinuous. Total protein/ creatinine in urine of rats 30 min and 24 h after exercise is significantly higher than that of group C(p<0.01), and group EA is slightly retuned and lower than group EI(p<0.05). Compared with group C, Serum Testosterone/Corticosterone of rats in group EI and EA is significantly decreased, and there is a significant difference (p<0.01). However, there is a signi
目的淤泥膜是肾脏滤过屏障中最重要、最具生物活性的膜结构,淤泥膜结构和功能异常是蛋白尿的根本原因。到目前为止,关于过度训练引起的泥沙隔膜变化的文献很少。本研究建立长期高强度跑步机运动引起的运动性蛋白尿大鼠模型,模拟运动员周期内的进行性负荷训练。观察运动后即刻及24 h肾脏的组织学和超微结构变化,分析持续性蛋白尿发生时淤泥膜的变化规律。方法选择36只Sprague-Dawley大鼠,随机分为3组:对照组(C组,12只),运动后立即抽取组(EI组,12只),运动后24 h抽取组(EA组,12只)。C组不训练。EI组和EA组大鼠在负荷递增的跑步机上训练6周(10%分级,6 d/w):第一周以10 m/min的速度跑步10 min。从第二周开始,跑步速度增加5m/min/w,训练时间增加30min/w。在最后一周,如果老鼠不能保持目标强度,它们就会筋疲力尽。记录大鼠疲劳时间,运动后即刻和24小时分别取EI组和EA组。光学显微镜下观察肾小球的组织学变化,透射电镜下观察淤膜的超微结构。BCA法检测尿总蛋白,Jaffe法检测血清和尿肌酐,放射免疫法检测血清睾酮和皮质酮,两点动态法检测血清尿素,western-blot检测Nephrin的表达。结果EI组和EA组大鼠在训练第一个周末体重逐渐下降,第三个周末至训练结束体重下降明显,与C组比较差异有统计学意义(p0.05)。结论大鼠因高强度训练引起过度训练综合征,并伴有持续性蛋白尿,24 h后肾功能紊乱,不能有效恢复。与此同时,大鼠的肾脏形态和超微结构发生“致病性样”变化,且随恢复时间的延长不显著降低。结果表明,过度训练引起的肾结构和粉膜超微结构损伤是运动性蛋白尿的结构基础。
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引用次数: 0
PO-055 Changes of trace elements in skeletal muscle and serum of rats after exercise-induced injury PO-055运动损伤大鼠骨骼肌及血清中微量元素的变化
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I3.10753
Jingyun Liu, Qun Zuo
Objective This study is to investigate the changes of trace elements (Cu, Fe, Zn, Se, Mg) in serum and skeletal muscle of rats after skeletal muscle injury induced by downhill running, and to find out the change regularity of trace elements in the body after exercise injury. To provide experimental basis for how to use trace elements supplements reasonably. Methods Fifty-four healthy male Sprague-Dawley rats aged 8 weeks were randomly divided into two groups: control group (C, N=6) and exercise group (E, N=48, include: 0 h group, 6 h group, 12 h group, 24 h group, 48 h group, 72 h group, 1- week group and 2- week group). The rats in exercise groups run down a 16°incline at 16m/min for 90 minutes. At the end of the exercise, the rats were killed at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 1 week and 2 weeks, respectively. The serum was got from the inferior vena cava blood and diluted by 1% nitric acid. The muscle was got from the right side of the rat's sural which were digested by concentrated nitric acid and 30% hydrogen peroxide in 75℃water bath for 20mins. The content of trace elements in muscle and serum were measured by inductively coupled plasma atomic emission spectrometry (ICP-MS). All the data are analyzed and processed by SPSS22.0 statistical software. Results (1) The contents of trace elements in serum showed: Cu, Zn, Mg, Se decreased immediately after exercise, but the Cu still increased to reach a peak at 24h after decreasing, and after 2 weeks the content of Cu was slightly lower than pre-exercise level. However, the content of Zn did not elevate again, it continued declined to the lowest at 24h which was significantly lower than control group (P < 0.05). And after 2 weeks, Zn did not return to the pre-exercise level. The changes of Mg, Se in serum was not statistically significant. There is no difference between 0h and control groups in content of Fe, after that Fe decreased continually and appeared the least value at 24h, the differences between immediate group and control group were statistically significant (P < 0.05). Fe returned to the pre-exercise level after 2 weeks. (2) The contents of trace elements in muscle showed: Most of trace elements increased to the maximum level at 6 h, after that Mg, Fe, Cu decreased to the lowest value at 72 h which were significant lower than 0h group or 6h group (P < 0. 05). ALL the trace elements were lower than pre-exercise level. There was no statistical difference in the content of Se in muscle. Conclusions (1) The different changes of trace elements in skeletal muscle and serum after exercise injury may be due to the redistribution of trace elements caused by the body adaptability. (2) The most obviously changes of trace element in serum and muscle are Cu and Zn. Both of them did not return to the pre-exercise level after 2 weeks, it suggests that the supplement include Cu and Zn may play an important role in recovering after exercise-induced injury.
目的研究大鼠下坡跑步所致骨骼肌损伤后血清和骨骼肌中微量元素(Cu、Fe、Zn、Se、Mg)的变化,探讨运动损伤后体内微量元素的变化规律。为合理使用微量元素补充剂提供实验依据。方法将54只8周龄健康雄性Sprague-Dawley大鼠随机分为对照组(C, N=6)和运动组(E, N=48,包括:0 h组、6 h组、12 h组、24 h组、48 h组、72 h组、1周组和2周组)。运动组大鼠以16米/分钟的速度跑下16°斜坡90分钟。运动结束后,分别于0 h、6 h、12 h、24 h、48 h、72 h、1周、2周处死大鼠。取下腔静脉血,用1%硝酸稀释血清。取大鼠右侧腓肠肌,用浓硝酸和30%过氧化氢在75℃水浴中消化20min。采用电感耦合等离子体原子发射光谱法(ICP-MS)测定肌肉和血清中微量元素的含量。所有数据均采用SPSS22.0统计软件进行分析处理。结果(1)血清微量元素含量显示:运动后Cu、Zn、Mg、Se立即下降,但Cu仍在下降后24h达到峰值,2周后Cu含量略低于运动前水平。但锌含量不再升高,在24h时继续下降至最低,显著低于对照组(P < 0.05)。2周后,Zn未恢复到运动前水平。血清Mg、Se含量变化无统计学意义。0h组与对照组铁含量无显著差异,之后铁含量持续下降,在24h出现最低值,即刻组与对照组差异有统计学意义(P < 0.05)。2周后Fe恢复到运动前水平。(2)肌肉中微量元素含量显示:大部分微量元素在6h时达到最大值,之后Mg、Fe、Cu在72 h时降至最低,均显著低于0h组和6h组(P < 0.05)。05). 所有微量元素均低于运动前水平。肌肉中硒含量差异无统计学意义。结论(1)运动损伤后骨骼肌和血清中微量元素的不同变化可能与机体适应性引起的微量元素的重新分配有关。(2)血清和肌肉中微量元素变化最明显的是Cu和Zn。两周后均未恢复到运动前水平,提示补充Cu和Zn可能对运动损伤后的恢复有重要作用。
{"title":"PO-055 Changes of trace elements in skeletal muscle and serum of rats after exercise-induced injury","authors":"Jingyun Liu, Qun Zuo","doi":"10.14428/EBR.V1I3.10753","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.10753","url":null,"abstract":"Objective This study is to investigate the changes of trace elements (Cu, Fe, Zn, Se, Mg) in serum and skeletal muscle of rats after skeletal muscle injury induced by downhill running, and to find out the change regularity of trace elements in the body after exercise injury. To provide experimental basis for how to use trace elements supplements reasonably. \u0000Methods Fifty-four healthy male Sprague-Dawley rats aged 8 weeks were randomly divided into two groups: control group (C, N=6) and exercise group (E, N=48, include: 0 h group, 6 h group, 12 h group, 24 h group, 48 h group, 72 h group, 1- week group and 2- week group). The rats in exercise groups run down a 16°incline at 16m/min for 90 minutes. At the end of the exercise, the rats were killed at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 1 week and 2 weeks, respectively. The serum was got from the inferior vena cava blood and diluted by 1% nitric acid. The muscle was got from the right side of the rat's sural which were digested by concentrated nitric acid and 30% hydrogen peroxide in 75℃water bath for 20mins. The content of trace elements in muscle and serum were measured by inductively coupled plasma atomic emission spectrometry (ICP-MS). All the data are analyzed and processed by SPSS22.0 statistical software. \u0000Results (1) The contents of trace elements in serum showed: Cu, Zn, Mg, Se decreased immediately after exercise, but the Cu still increased to reach a peak at 24h after decreasing, and after 2 weeks the content of Cu was slightly lower than pre-exercise level. However, the content of Zn did not elevate again, it continued declined to the lowest at 24h which was significantly lower than control group (P < 0.05). And after 2 weeks, Zn did not return to the pre-exercise level. The changes of Mg, Se in serum was not statistically significant. There is no difference between 0h and control groups in content of Fe, after that Fe decreased continually and appeared the least value at 24h, the differences between immediate group and control group were statistically significant (P < 0.05). Fe returned to the pre-exercise level after 2 weeks. (2) The contents of trace elements in muscle showed: Most of trace elements increased to the maximum level at 6 h, after that Mg, Fe, Cu decreased to the lowest value at 72 h which were significant lower than 0h group or 6h group (P < 0. 05). ALL the trace elements were lower than pre-exercise level. There was no statistical difference in the content of Se in muscle. \u0000Conclusions (1) The different changes of trace elements in skeletal muscle and serum after exercise injury may be due to the redistribution of trace elements caused by the body adaptability. (2) The most obviously changes of trace element in serum and muscle are Cu and Zn. Both of them did not return to the pre-exercise level after 2 weeks, it suggests that the supplement include Cu and Zn may play an important role in recovering after exercise-induced injury.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85741018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PO-265 Acute whole-body vibration increases energy expenditure and skeletal muscle microvascular perfusion 急性全身振动增加能量消耗和骨骼肌微血管灌注
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I5.11143
A. Betik, L. Parker, Gunveen Kaur, M. Keske
Objective Insulin resistance and cardiometabolic disease are associated with decreased muscle microvascular perfusion which impairs nutrient delivery to the muscle.  Impaired glucose and insulin delivery contributes to lower glucose uptake into the skeletal muscle. Exercise increases muscle microvascular perfusion, however, cardiometabolic disease patients are not always willing or able to undertake regular exercise. Objective: Whole-body vibration (WBV) has previously been shown to increase energy expenditure and limb bulk blood flow, however whether WBV increases skeletal muscle microvascular perfusion is not known.   Methods Methods: Eleven healthy participants (5 males, 6 females; Age: 33 ± 1.9 years) stood on a WBV platform (Galileo Sport, Novotec Medical GmbH, Pforzheim, Germany) for 3 min at 12.5 Hz which was compared to standing without vibration.  Thigh muscle (vastus lateralis) microvascular perfusion was assessed by contrast-enhanced ultrasound (iU22, Philips Medical, North Ryde, NSW, Australia) by infusing Definity® microbubbles (Lantheus Medical Imaging, N. Billeruca, USA) intravenously and measured for 3 min following WBV.  Oxygen consumption (Metamax, Cortex Biophysik GmbH, Leipzig, Germany) was measured while standing prior to WBV and during the third minute of WBV. Results Results: Compared with standing without vibration, 3 min of WBV more than doubled muscle microvascular perfusion (0.73 ± 0.17 vs 2.87 ± 0.81 AI/sec, p<0.05) which remained elevated above baseline for 3 min after cessation of WBV. Oxygen consumption modestly but significantly increased while undergoing WBV (282 ± 0.013 vs 419 ± 0.023 mL/min, p<0.05). Conclusions Conclusion: This is the first study to show that WBV significantly increases muscle microvascular perfusion in healthy adults.  We are currently undertaking studies to determine if this WBV may be of benefit in populations with impaired microvascular perfusion, such as type 2 diabetes, for improving cardiometabolic health.  
目的胰岛素抵抗和心脏代谢疾病与肌肉微血管灌注减少有关,这损害了肌肉的营养输送。葡萄糖和胰岛素输送受损有助于降低骨骼肌对葡萄糖的摄取。运动增加了肌肉微血管灌注,然而,心脏代谢疾病患者并不总是愿意或能够进行定期运动。目的:全身振动(WBV)先前已被证明可以增加能量消耗和肢体大血流量,但WBV是否会增加骨骼肌微血管灌注尚不清楚。方法:11名健康受试者(男5名,女6名;年龄:33±1.9岁)在WBV平台(Galileo Sport, Novotec Medical GmbH, Pforzheim, Germany)以12.5 Hz频率站立3分钟,与无振动站立进行比较。通过静脉注入Definity®微泡(Lantheus Medical Imaging, N. Billeruca, USA),通过对比增强超声(iU22, Philips Medical, North Ryde, NSW, Australia)评估大腿肌肉(股侧肌)微血管灌注,并在WBV后3分钟测量。氧气消耗(Metamax, Cortex Biophysik GmbH, Leipzig, Germany)在站立时和站立后的第三分钟测量。结果:与无振动站立时相比,3 min WBV时肌肉微血管灌注(0.73±0.17 vs 2.87±0.81 AI/sec, p<0.05)增加一倍以上,且停止WBV后3 min仍高于基线水平。耗氧量适度但显著增加(282±0.013 mL/min vs 419±0.023 mL/min, p<0.05)。结论:这是第一次有研究表明WBV能显著增加健康成人肌肉微血管灌注。我们目前正在进行研究,以确定这种WBV是否可能有益于微血管灌注受损的人群,如2型糖尿病,以改善心脏代谢健康。
{"title":"PO-265 Acute whole-body vibration increases energy expenditure and skeletal muscle microvascular perfusion","authors":"A. Betik, L. Parker, Gunveen Kaur, M. Keske","doi":"10.14428/EBR.V1I5.11143","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.11143","url":null,"abstract":"Objective Insulin resistance and cardiometabolic disease are associated with decreased muscle microvascular perfusion which impairs nutrient delivery to the muscle.  Impaired glucose and insulin delivery contributes to lower glucose uptake into the skeletal muscle. Exercise increases muscle microvascular perfusion, however, cardiometabolic disease patients are not always willing or able to undertake regular exercise. Objective: Whole-body vibration (WBV) has previously been shown to increase energy expenditure and limb bulk blood flow, however whether WBV increases skeletal muscle microvascular perfusion is not known.   \u0000Methods Methods: Eleven healthy participants (5 males, 6 females; Age: 33 ± 1.9 years) stood on a WBV platform (Galileo Sport, Novotec Medical GmbH, Pforzheim, Germany) for 3 min at 12.5 Hz which was compared to standing without vibration.  Thigh muscle (vastus lateralis) microvascular perfusion was assessed by contrast-enhanced ultrasound (iU22, Philips Medical, North Ryde, NSW, Australia) by infusing Definity® microbubbles (Lantheus Medical Imaging, N. Billeruca, USA) intravenously and measured for 3 min following WBV.  Oxygen consumption (Metamax, Cortex Biophysik GmbH, Leipzig, Germany) was measured while standing prior to WBV and during the third minute of WBV. \u0000Results Results: Compared with standing without vibration, 3 min of WBV more than doubled muscle microvascular perfusion (0.73 ± 0.17 vs 2.87 ± 0.81 AI/sec, p<0.05) which remained elevated above baseline for 3 min after cessation of WBV. Oxygen consumption modestly but significantly increased while undergoing WBV (282 ± 0.013 vs 419 ± 0.023 mL/min, p<0.05). \u0000Conclusions Conclusion: This is the first study to show that WBV significantly increases muscle microvascular perfusion in healthy adults.  We are currently undertaking studies to determine if this WBV may be of benefit in populations with impaired microvascular perfusion, such as type 2 diabetes, for improving cardiometabolic health. \u0000 ","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85868635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PO-161 Effect of different type of exercise on mitochondrial homeostasis in rats with myocardial infarction PO-161不同运动方式对心肌梗死大鼠线粒体稳态的影响
Pub Date : 2018-10-04 DOI: 10.14428/ebr.v1i4.11883
Hai Bo, Yong Zhang
Objective To investigate the different effect of moderate-intensity continuous training (MCT) and high-intensity interval exercise training (HIT) on ventricular remodeling and mitochondrial homeostasis after acute myocardial infarction (AMI). Methods The AMI rat model was achieved by ligating coronary artery. The AMI and sham operation rats were randomly divided into four groups: sham operation group (Sham), AMI control group (AMI), AMI MCT group (AMI+M), and AMI HIT group (AMI+H). Animals in the AMI+M and AMI+H groups underwent 4 weeks MCT and HIT respectively. Five weeks after AMI, hemodynamic changes, mitochondrial bioenergetics, and PINK1, Beclin1, Mfn2, Drp1, Tfam, COXⅣ, PGC-1α were detected. Results Comparing with AMI group, in AMI+M and AMI+H groups, Beclin1 (146.33±18.47, 143.28±16.96.vs. 123.27±13.27, P<0.05), PINK1 (150.33±20.54, 152.28±18.34.vs. 125.27±17.67, P<0.05), Mfn2 (122.28±18.81, 117.19±17.04.vs. 46.27±6.72, P<0.01), and PGC-1α (82.15±16.58, 102.25±13.27.vs. 60.27±9.36, P<0.05~0.01) expression elevated significantly, whereas ROS generation (122.28±18.81, 117.19±17.04.vs. 46.27±6.72, P<0.01) and Drp1 expression (9.58±1.40, 10.18±1.37.vs. 15.85±1.61, P<0.05) showed dramatic decrease. In addition, in AMI+H group, +dp/dt max (6326±325.vs. 5368±271, P<0.05), -dp/dt max (-5312±246.vs. -4457±250, P<0.05), mitochondrial membrane potential (85.24±11.94.vs. 71.28±8.34, P<0.05), ATP synthesis activity (38.77±5.16.vs. 32.33±4.14, P<0.05), Tfam (95.25±12.05.vs. 78.27±12.22, P<0.05) and COXⅣ (89.25±14.06.vs. 67.27±13.71, P<0.05) expression improved significantly. Comparing with AMI+M group, in AMI+H group, +dp/dt max (6326±325.vs. 5775±310, P<0.05), -dp/dt max (-5312±246.vs. -4778±305, P<0.05), PGC-1α (102.25±13.27.vs. 82.15±16.58 1, P<0.05), Tfam (95.25±12.05.vs. 80.15±11.99, P<0.05) and COXⅣ(89.25±14.06.vs. 72.15±10.89, P<0.05) expression improved significantly. Conclusions High-intensity interval exercise training is superior to moderate-intensity continuous training for ameliorating ventricular remodeling and mitochondrial homeostasis after acute myocardial infarction.
目的探讨中强度连续训练(MCT)和高强度间歇运动训练(HIT)对急性心肌梗死(AMI)后心室重构和线粒体稳态的不同影响。方法采用结扎冠状动脉法制备AMI大鼠模型。将AMI和假手术大鼠随机分为4组:假手术组(sham)、AMI对照组(AMI)、AMI MCT组(AMI+M)、AMI HIT组(AMI+H)。AMI+M组和AMI+H组分别进行4周的MCT和HIT治疗。AMI后5周,检测血流动力学变化、线粒体生物能量学及PINK1、Beclin1、Mfn2、Drp1、Tfam、COXⅣ、PGC-1α。结果与AMI组比较,AMI+M组和AMI+H组Beclin1(146.33±18.47)、(143.28±16.96);PINK1(150.33±20.54,152.28±18.34);进行Mfn2 125.27±17.67,P < 0.05),(122.28±18.81,117.19±17.04.vs。46.27±6.72,P < 0.01), PGC-1α(82.15±16.58,102.25±13.27.vs。(60.27±9.36,P<0.05~0.01)显著升高,而ROS生成(122.28±18.81,117.19±17.04,P<0.05)显著升高。46.27±6.72,P<0.01)和Drp1表达量(9.58±1.40,10.18±1.37);15.85±1.61,P<0.05)显著降低。AMI+H组+dp/dt max(6326±325 vs.;-dp/dt max(-5312±246),P<0.05;-4457±250,P<0.05),线粒体膜电位(85.24±11.94.vs。71.28±8.34,P<0.05), ATP合成活性(38.77±5.16);32.33±4.14 (P<0.05), Tfam(95.25±12.05);78.27±12.22,P<0.05), COXⅣ(89.25±14.06);67.27±13.71,P<0.05)。AMI+H组与AMI+M组比较,+dp/dt max(6326±325)vs。-dp/dt max(-5312±246.vs., P<0.05);-4778±305,P<0.05), PGC-1α(102.25±13.27);82.15±16.58 1,P<0.05), Tfam(95.25±12.05);80.15±11.99,P<0.05), COXⅣ(89.25±14.06);72.15±10.89,P<0.05)表达明显改善。结论高强度间歇运动训练在改善急性心肌梗死后心室重构和线粒体稳态方面优于中等强度连续运动训练。
{"title":"PO-161 Effect of different type of exercise on mitochondrial homeostasis in rats with myocardial infarction","authors":"Hai Bo, Yong Zhang","doi":"10.14428/ebr.v1i4.11883","DOIUrl":"https://doi.org/10.14428/ebr.v1i4.11883","url":null,"abstract":"Objective To investigate the different effect of moderate-intensity continuous training (MCT) and high-intensity interval exercise training (HIT) on ventricular remodeling and mitochondrial homeostasis after acute myocardial infarction (AMI). \u0000Methods The AMI rat model was achieved by ligating coronary artery. The AMI and sham operation rats were randomly divided into four groups: sham operation group (Sham), AMI control group (AMI), AMI MCT group (AMI+M), and AMI HIT group (AMI+H). Animals in the AMI+M and AMI+H groups underwent 4 weeks MCT and HIT respectively. Five weeks after AMI, hemodynamic changes, mitochondrial bioenergetics, and PINK1, Beclin1, Mfn2, Drp1, Tfam, COXⅣ, PGC-1α were detected. \u0000Results Comparing with AMI group, in AMI+M and AMI+H groups, Beclin1 (146.33±18.47, 143.28±16.96.vs. 123.27±13.27, P<0.05), PINK1 (150.33±20.54, 152.28±18.34.vs. 125.27±17.67, P<0.05), Mfn2 (122.28±18.81, 117.19±17.04.vs. 46.27±6.72, P<0.01), and PGC-1α (82.15±16.58, 102.25±13.27.vs. 60.27±9.36, P<0.05~0.01) expression elevated significantly, whereas ROS generation (122.28±18.81, 117.19±17.04.vs. 46.27±6.72, P<0.01) and Drp1 expression (9.58±1.40, 10.18±1.37.vs. 15.85±1.61, P<0.05) showed dramatic decrease. In addition, in AMI+H group, +dp/dt max (6326±325.vs. 5368±271, P<0.05), -dp/dt max (-5312±246.vs. -4457±250, P<0.05), mitochondrial membrane potential (85.24±11.94.vs. 71.28±8.34, P<0.05), ATP synthesis activity (38.77±5.16.vs. 32.33±4.14, P<0.05), Tfam (95.25±12.05.vs. 78.27±12.22, P<0.05) and COXⅣ (89.25±14.06.vs. 67.27±13.71, P<0.05) expression improved significantly. Comparing with AMI+M group, in AMI+H group, +dp/dt max (6326±325.vs. 5775±310, P<0.05), -dp/dt max (-5312±246.vs. -4778±305, P<0.05), PGC-1α (102.25±13.27.vs. 82.15±16.58 1, P<0.05), Tfam (95.25±12.05.vs. 80.15±11.99, P<0.05) and COXⅣ(89.25±14.06.vs. 72.15±10.89, P<0.05) expression improved significantly. \u0000Conclusions High-intensity interval exercise training is superior to moderate-intensity continuous training for ameliorating ventricular remodeling and mitochondrial homeostasis after acute myocardial infarction.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85901562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
OR-045 Comparative Study on Physical Fitness in Female College Students between Different BFP Grades 不同BFP年级女大学生体质比较研究
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I2.9743
Hui Wang, Chognlin Zhang, Xiaomin Ding, Shaosheng Liu
Objective  Through testing the body fat percentage (BFP) and physical fitness by laboratory methods in female college students, the relationship between physical fitness and body morphology of female college students was revealed.  Methods BFP and fat free mass were measured among 633 healthy female college students randomly selected. All subjects were divided into five groups by their BFP: low, normal, high body fat, grade I obesity and grade II/III obesity. Grip Strength, Leg Strength, Back Strength, Vital Capacity, Maximal Oxygen Uptake, Reaction Time, Sit And Reach, Back Scratch and Standing On One Foot With Closed Eyes were tested.  Results There were significant differences between all groups in Grip Strength, Back Strength, Vital Capacity, the absolute and relative value of VO2max, Back Scratch on both sides and Standing on One Foot with Closed Eyes. The difference in Grip Strength and Back Strength showed significantly (P < 0.05), and the others showed very significant difference (P < 0.01). There was no significant difference in Resting Heart Rate, Reaction Time, Sit And Reach and Leg Strength. The results of multiple comparison showed that compared with 4 group,there were significant differences in Vital Capacity, relative value of Maximum Oxygen Uptake, Back Scratch in both sides in 2 group and 3 group. Moreover, compared with 5 group, there were significant differences in all the indicators in 2 group and 3 group. And there were significant differences in all the indicators except Back Strength and the relative value of Maximum Oxygen Uptake between 5 group and 4 group. Conclusions The results showed that the physical fitness of female college students with normal BFP was significantly better than that of obese ones. The major influence of exceeding 30 percent in BFP was the cardiopulmonary endurance fitness and upper limb flexibility fitness. In the group of BFP higher than 35%, these influences were more significant, meanwhile, balance fitness declined.
目的通过对女大学生体脂率(BFP)和体质的实验室测定,揭示女大学生体质与身体形态的关系。方法随机抽取633名健康女大学生,测定BFP和无脂肪量。所有受试者按BFP分为5组:低体脂、正常体脂、高体脂、I级肥胖和II/III级肥胖。测试了握力、腿部力量、背部力量、肺活量、最大摄氧量、反应时间、坐姿和伸展、背部抓伤和闭着眼睛单脚站立。结果各组在握力、背部力量、肺活量、最大摄氧量绝对值和相对值、双侧背部抓痕、闭眼单脚站立等指标上差异均有统计学意义。握力、背力差异极显著(P < 0.05),其余差异极显著(P < 0.01)。在静息心率、反应时间、坐姿和伸展以及腿部力量方面没有显著差异。多重比较结果显示,与4组比较,2组和3组在肺活量、最大摄氧量相对值、两侧背部划痕等指标上均有显著差异。与5组比较,2组、3组各项指标均有显著差异。除背强度和最大摄氧量的相对值外,5组与4组在其他指标上均有显著差异。结论BFP正常的女大学生体质明显优于肥胖女大学生。BFP超过30%的主要影响因素是心肺耐力和上肢柔韧性。在BFP高于35%组,这些影响更为显著,平衡适合度下降。
{"title":"OR-045 Comparative Study on Physical Fitness in Female College Students between Different BFP Grades","authors":"Hui Wang, Chognlin Zhang, Xiaomin Ding, Shaosheng Liu","doi":"10.14428/EBR.V1I2.9743","DOIUrl":"https://doi.org/10.14428/EBR.V1I2.9743","url":null,"abstract":"Objective  Through testing the body fat percentage (BFP) and physical fitness by laboratory methods in female college students, the relationship between physical fitness and body morphology of female college students was revealed.  \u0000Methods BFP and fat free mass were measured among 633 healthy female college students randomly selected. All subjects were divided into five groups by their BFP: low, normal, high body fat, grade I obesity and grade II/III obesity. Grip Strength, Leg Strength, Back Strength, Vital Capacity, Maximal Oxygen Uptake, Reaction Time, Sit And Reach, Back Scratch and Standing On One Foot With Closed Eyes were tested.  \u0000Results There were significant differences between all groups in Grip Strength, Back Strength, Vital Capacity, the absolute and relative value of VO2max, Back Scratch on both sides and Standing on One Foot with Closed Eyes. The difference in Grip Strength and Back Strength showed significantly (P < 0.05), and the others showed very significant difference (P < 0.01). There was no significant difference in Resting Heart Rate, Reaction Time, Sit And Reach and Leg Strength. The results of multiple comparison showed that compared with 4 group,there were significant differences in Vital Capacity, relative value of Maximum Oxygen Uptake, Back Scratch in both sides in 2 group and 3 group. Moreover, compared with 5 group, there were significant differences in all the indicators in 2 group and 3 group. And there were significant differences in all the indicators except Back Strength and the relative value of Maximum Oxygen Uptake between 5 group and 4 group. \u0000Conclusions The results showed that the physical fitness of female college students with normal BFP was significantly better than that of obese ones. The major influence of exceeding 30 percent in BFP was the cardiopulmonary endurance fitness and upper limb flexibility fitness. In the group of BFP higher than 35%, these influences were more significant, meanwhile, balance fitness declined.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86020890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PO-031 Research on Tapering to Elite Female Race Walking Players with Altitude Training Characteristics
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I3.9963
N. Zhu, Q. Tang, Xiuyun Li
Objective The ultimate goal of athletic training is to perform the optimum competitive states in the competitions. The research has shown that one of the key factors to achieve the goal is effective and appropriate Tapering in preparing period. By systematically and completely tracing and summarizing the Tapering in endurance events with altitude training at the entire training period which could provide a better guidance for the training and bring into an furthest effects, providing a real reference cases for achieving competitive targets and scientific training. Methods Have Selected 20 players and 10 games from annual competitions (National Championships, the National Games, World Games, Olympic qualifiers etc.) from 2011 to 2017, which involved 9 players who are the international master athletes and 11 players who are the National master athletes with 4-10 years of training. According to entire preparing period schedule and training targets to analyze the intensity, volume and rhythm of training, recording the athletes whose Hb(hemoglobin) number in different circulatory sports and concluding the athlete’s Tapering mode and effects. Results 1. The systematical time of preparing for competition was 65±5 days; 2. The entire training arrangements comprised of four stages: the plain training preparation phase, the altitude training phase, the plain training, pre-competition training phase; 3. Applied single peak and single cycle training model, the training structure was cycling 12 to 15 to 10 to 15 to 26kms, and the maximum training interval was 5-7 days after highest intensity training. 4. The tapering was engaged in the first week before the altitude training phase and 10-12days before competition; 5. The training load decreased 10-15%,the training intensity decreased by 25%-30% and the training volume was maintained to 90-105% during the first Tapering which mainly decrease training intensity; 6. During the second Tapering, the training load decreased by 40-60%, the training intensity was 90%-95% and at the key class, training intensity was more than 100% which mainly decrease training volume; 7. The training frequency was kept at 95-105% at two Tapering training phases; 8. Athletes’ Hb number was maintained at  132.04 ±6.53IU. The whole adjustments varied with the Tapering training arrangements. Conclusions 1. There were two Tapering training models with altitude training characteristics before the preparing competition period; 2. The one Tapering was decreasing training volume passively at the beginning of the altitude training, and the another Tapering was decreasing volume initiatively before the competition. 3. The first Tapering was to reduce the intensity of training, and the second Tapering was to reduce the volume of training; 4. The first Tapering is last 5 to 7days, and the second Tapering last 10 to 12days; 5. The entire preparing preformed as hyperbolic mode. The first tapering was the exponential tapering(slow decay), and the
目的运动训练的最终目的是在比赛中表现出最佳的竞技状态。研究表明,在准备阶段进行有效、适当的减径是实现这一目标的关键因素之一。通过系统、完整地跟踪和总结高原训练耐力项目在整个训练期间的减径,可以更好地指导训练,发挥最大的效果,为实现竞技目标和科学训练提供真实的参考案例。方法选取2011 - 2017年全国锦标赛、全运会、世锦赛、奥运会预选赛等年度比赛的20名运动员和10个项目,其中国际运动健将9名,国家运动健将11名,训练时间为4-10年。根据整个备战期计划和训练目标,分析训练的强度、运动量和节奏,记录运动员在不同循环运动中的血红蛋白(Hb)数,总结运动员的减持方式和效果。结果1。比赛系统准备时间为65±5天;2. 整个训练安排分为四个阶段:平原训练准备阶段、高原训练阶段、平原训练阶段、赛前训练阶段;3.采用单峰单周期训练模式,训练结构为骑行12 ~ 15 ~ 10 ~ 15 ~ 26km,最大训练间隔为最高强度训练后5 ~ 7天。4. 在高原训练前1周和比赛前10-12天进行减量;5. 第一次减持期间,训练负荷降低10 ~ 15%,训练强度降低25% ~ 30%,训练量维持在90 ~ 105%,主要是降低训练强度;6. 第二次减负时,训练负荷减少40 ~ 60%,训练强度为90% ~ 95%,关键课时训练强度大于100%,主要是训练量减少;7. 在两个渐缩训练阶段,训练频率保持在95-105%;8. 运动员Hb维持在132.04±6.53IU。整个调整随着逐渐减少的训练安排而变化。结论1。赛前有两种具有高原训练特征的锥形训练模式;2. 一种是在高原训练开始时被动减少训练量,另一种是在比赛前主动减少训练量。3.第一个逐渐减少是为了减少训练强度,第二个逐渐减少是为了减少训练量;4. 第一次减持持续5 ~ 7天,第二次减持持续10 ~ 12天;5. 整个制备过程以双曲模式进行。第一次递减为指数递减(缓慢衰减),第二次递减为指数递减(快速衰减);6. 运动员的功能能力随锥度的变化而变化。适应能力越快,竞争状态越好。
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引用次数: 0
OR-041 Infection of Different Altitudes on College Students’ Body Shape OR-041不同海拔对大学生形体的影响
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I2.9703
Xiaoxia Qiu, Jin Zhao
Objective Considering that guizhou is located in a plateau region with various gradient altitude,and we have never seen any relevant report on the study of different altitudes influence the students'physical fitness at home or abroad. the aim of this research is the author runs a normal physical fitness test on native 774 non-PE major students who all comes from different altitudes 774, so as to provide reference standard for promoting the health of the students group. Methods literature data, fitness testing, statistic, and logic analysis. Results 1、There was no obvious consistency between the height change of students in different atitudes indicating that the influence of different altitude environments on the height of students was not obvious.2、Most of the students on the plateau show the thickness of the skinfold is the thickest , while the thickness of the skinfold of students on the plain and the subplateau is different and irregular. 3. There was no significant difference in the chest circumference of the four groups of students at three altitudes, indicating that the environment at different altitudes had no significant influence on the development of the respiratory organs and chest muscles of the students. 4. The waist circumference of the three groups showed no obvious pattern, indicating that the environment at different altitude had no significant influence on the waist circumference. 5. The waist-hip range of female students on the plateau is relatively high, while other students are in the ideal range. 6. The BMI of all the students in the three places is within the range of 18.5 ~ 22.9, that is, all the students in the three places are in the normal range. Conclusions altitude has no significant effect on students'height, chest circumference, waist circumference and BMI. The thickness of skinfold of students on plateau is thicker than that of plain and subplateau students. The waist-hip range of female students on the plateau is relatively high, while other students are in the ideal range.
目的考虑到贵州地处高原地区,海拔梯度各不相同,国内外尚无关于不同海拔对学生体质影响研究的相关报道。本研究的目的是对来自不同海拔地区的本土774非体育专业学生进行正常体质测试,为促进学生群体的健康提供参考标准。方法采用文献资料、适应度检验、统计学和逻辑分析。结果1、不同海拔地区学生身高变化不存在明显的一致性,说明不同海拔环境对学生身高的影响不明显。2、高原上的学生多数表现为皮褶厚度最厚,而平原和亚高原上的学生皮褶厚度不同且不规则。3.三种海拔高度四组学生的胸围无显著差异,说明不同海拔环境对学生呼吸器官和胸肌发育无显著影响。4. 三组的腰围没有明显的规律,说明不同海拔环境对腰围的影响不显著。5. 高原女生腰臀范围比较大,其他同学都在理想范围内。6. 三地学生的BMI均在18.5 ~ 22.9范围内,即三地学生均在正常范围内。结论海拔对学生身高、胸围、腰围、BMI均无显著影响。高原学生的皮褶厚度比平原和亚高原学生要厚。高原女生腰臀范围比较大,其他同学都在理想范围内。
{"title":"OR-041 Infection of Different Altitudes on College Students’ Body Shape","authors":"Xiaoxia Qiu, Jin Zhao","doi":"10.14428/EBR.V1I2.9703","DOIUrl":"https://doi.org/10.14428/EBR.V1I2.9703","url":null,"abstract":"Objective Considering that guizhou is located in a plateau region with various gradient altitude,and we have never seen any relevant report on the study of different altitudes influence the students'physical fitness at home or abroad. the aim of this research is the author runs a normal physical fitness test on native 774 non-PE major students who all comes from different altitudes 774, so as to provide reference standard for promoting the health of the students group. \u0000Methods literature data, fitness testing, statistic, and logic analysis. \u0000Results 1、There was no obvious consistency between the height change of students in different atitudes indicating that the influence of different altitude environments on the height of students was not obvious.2、Most of the students on the plateau show the thickness of the skinfold is the thickest , while the thickness of the skinfold of students on the plain and the subplateau is different and irregular. 3. There was no significant difference in the chest circumference of the four groups of students at three altitudes, indicating that the environment at different altitudes had no significant influence on the development of the respiratory organs and chest muscles of the students. 4. The waist circumference of the three groups showed no obvious pattern, indicating that the environment at different altitude had no significant influence on the waist circumference. 5. The waist-hip range of female students on the plateau is relatively high, while other students are in the ideal range. 6. The BMI of all the students in the three places is within the range of 18.5 ~ 22.9, that is, all the students in the three places are in the normal range. \u0000Conclusions altitude has no significant effect on students'height, chest circumference, waist circumference and BMI. The thickness of skinfold of students on plateau is thicker than that of plain and subplateau students. The waist-hip range of female students on the plateau is relatively high, while other students are in the ideal range.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77180232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PO-181 The Comparative Study on the Maximum Oxygen Uptake Test of 10- Month Old Wistar Rats PO-181对10月龄Wistar大鼠最大摄氧量的比较研究
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I4.12723
Yanan Dong, F. Qin, Jiexiu Zhao, Songtao Wang, Min-xiao Xu, Zhongwei Wang
Objective The maximum oxygen uptake (VO2max) is an ideal index to objectively evaluate the cardiopulmonary function, as well as the basic to define exercise intensity. In the field of sports science, laboratory animals are often used to explore the effect and mechanism of exercise intervention. Therefore, it is very important to design optimal VO2max test protocol and to ensure the accuracy of VO2max according to the characteristics of the experimental animal itself. In this study, Wistar rats were selected, and various VO2max test protocols were designed and analyzed to screen out the optimal VO2max test protocol for the 10-month old wistar rats. Methods  20 SPF Wistar rats (male, 10 month old) were tested for maximal oxygen uptake by a four channel metabolic monitoring system and running treadmill. Five different test protocols were executed. Each rat completed five test protocols in random order with 3 days’ interval. The exercise performance (coordination degree, exhaustion state), oxygen uptake platform, finish time, VO2max and RER value were recorded during the test of each program, and the performance and test data were compared. Results  1) 12 rats completed all 5 test protocols of VO2max. The induction ratio of VO2max of P1 was only 58%, and P2 and P4 were 75%. While, the induction rate of P3 and P5 were both 83%. 2) For the Bedford improvement protocol (P1), due to the intense increased exercise load, the rats showed more intense stress, the less coordination degree, injured even death, and lower induction rate of VO2max. 3) The VO2max and RER values induced by the P5 are significantly higher than that of P1 (p<0.05). The finish time of P3 is significantly higher than that of P1 (p<0.01) and P5 (p<0.05). Conclusions For the VO2max test for middle aged rats, with the suitable speed of the running treadmill, the change of gradient should be as the main way of increasing load, or increasing the gradient of the slope firstly, which can obtained optimal VO2max, meanwhile reduce the stress response and the risk of injury and serious damage.
目的最大摄氧量(VO2max)是客观评价心肺功能的理想指标,也是确定运动强度的基础。在运动科学领域,经常使用实验动物来探索运动干预的效果和机制。因此,根据实验动物自身的特点,设计最佳的VO2max测试方案,保证VO2max的准确性是非常重要的。本研究选取Wistar大鼠,设计并分析各种VO2max测试方案,筛选出10月龄Wistar大鼠的最佳VO2max测试方案。方法采用四通道代谢监测系统和跑步机对20只10月龄雄性SPF级Wistar大鼠进行最大摄氧量测定。执行了五种不同的测试方案。每只大鼠按随机顺序完成5个试验方案,间隔3天。在每个项目的测试过程中记录运动表现(协调度、衰竭状态)、摄氧量平台、完成时间、VO2max和RER值,并将性能与测试数据进行比较。结果1)12只大鼠完成了5个VO2max测试方案。P1的VO2max诱导率仅为58%,P2和P4为75%。P3和P5的诱导率均为83%。2)对于贝德福德改善方案(P1),由于运动负荷的剧烈增加,大鼠表现出更强烈的应激,协调度降低,甚至受伤死亡,VO2max诱导率降低。3) P5诱导的VO2max和RER值显著高于P1 (p<0.05)。P3的完成时间显著高于P1 (p<0.01)和P5 (p<0.05)。结论对于中年大鼠VO2max测试,在适宜的跑步机速度下,应以坡度的变化为主要增加负荷的方式,或先增加坡度,这样可以获得最佳的VO2max,同时减少应激反应,降低损伤和严重损伤的风险。
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引用次数: 1
PO-286 The research about the evaluation index on cardiac reserve of female athletes PO-286女运动员心脏储备评价指标的研究
Pub Date : 2018-10-04 DOI: 10.14428/EBR.V1I5.13113
Yawen Du
Objective How well the heart and cardiovascular system operate will directly influence the ability that female athletes bear the training and competition load.The accurate evaluation of heart and cardiovascular system is the important part of female athletes training processes monitor and medical supervision. Nowadays, there are many evaluation index to assess the condition of the heart and cardiovascular system .Due to the differences of tested mechanisms ,there are huge differences lay between the focus of index to show the cardiac function  of  female athletes. As a result, to clarify the significance of different evaluation index will be benefit to accurately evaluate the cardiac function  of  female athletes. This research clarifies different significance of different index to evaluate female athletes’ cardiac function by comparing two indexes(CRI, HRr) to evaluate the cardiac contractility of female athletes in different programs ,and also offers grounds for accurately applying these two indexes to evaluate female athletes’ cardiac function. Methods The study subjects of this research are the female athletes from sports team in Hubei province. We select 63 female athletes whose ages are between 14 and 18 and average age is 17±1.6.They have been trained to play basketball game, soccer ,badminton or table tennis for move than four years. This research applies quantitative exercise experiment to get the cardiac index --CI2(CI=CO/BSA)after the female athletes’ doing sports .We can compare the difference between the cardiac indexes during the female athletes doing sports and they becoming calm and the work done by exercise load (PP) to get the cardiac reserve index(CRI) .The formula of this process is CRI=PP/(CI2- CI1)/10. We can also dividing CRI by cardiac function index to get heart recovery rate (HRr) .We divide CRI by CFI to get the value of HRr . CFI=(HR1+HR2+HR3-200)/10. Thereinto, HR1、HR2、HR3 respectively mean the athletes’ heart rates when they are calm ,when they just finish doing sports and when they are in restoration stage. Data assessment and statistical analysis was performed in this research ,using the value of female athletes’ CRI and HRr in different programs. Results There are huge differences between the average CRI values of female athletes in different programs. Among them, the highest value is 11.13±1.1,which is the average CRI values of female athletes who play soccer .And the lowest one is 7.56±0.7, which is the average CRI values of female athletes who play table tennis. The values of CRI of female athletes in basketball group and badminton group are 10.07±0.9 and 7.82±0.8 respectively. However, there is no significant difference between the values of female athletes’ HRr in different groups. The values are 0.94±0.05 (women’ s basketball) , 0.97±0.06 (women’ s football) ,0.93±0.04  (women’ s table tennis) ,0.97±0.08 (women’ s badminton) respectively. ;mso-font-kerning:1.0000pt;" >+HR2+HR3-200)/10. Thereinto, HR1、HR2、
目的心脏和心血管系统的运行状况将直接影响女运动员承受训练和比赛负荷的能力。心脏和心血管系统的准确评估是女运动员训练过程监测和医学监护的重要组成部分。目前,评价心脏和心血管系统状况的指标很多,但由于测试机制的不同,反映女运动员心功能的指标重点存在较大差异。因此,明确不同评价指标的意义,将有利于准确评价女运动员的心功能。本研究通过比较两项指标(CRI、HRr)对不同项目中女运动员心脏收缩力的评价,阐明了不同指标对评价女运动员心功能的不同意义,也为准确应用这两项指标评价女运动员心功能提供了依据。方法以湖北省运动队女运动员为研究对象。选取年龄在14 ~ 18岁之间的63名女运动员,平均年龄为17±1.6岁。他们接受过超过四年的篮球、足球、羽毛球或乒乓球训练。本研究采用定量运动实验的方法,得到了女运动员运动后的心脏指数——CI2(CI=CO/BSA),通过比较女运动员运动及平静状态时的心脏指数与运动负荷(PP)所做功的差值,得出了心脏储备指数(CRI),计算公式为:CRI=PP/(CI2- CI1)/10。我们也可以用CRI除以心功能指数得到心脏恢复速率(HRr),用CRI除以CFI得到HRr的值。CFI = (HR1上+ HR2 + hr3 - 200) / 10。其中,HR1、HR2、HR3分别表示运动员在平静状态下、刚结束运动时和恢复阶段的心率。本研究采用不同项目中女运动员的CRI和HRr值进行数据评估和统计分析。结果不同项目女运动员的平均CRI值存在较大差异。其中,最高为11.13±1.1,为足球女运动员的平均CRI值;最低为7.56±0.7,为乒乓球女运动员的平均CRI值。篮球组和羽毛球组女运动员的CRI分别为10.07±0.9和7.82±0.8。然而,不同组别的女运动员HRr值之间没有显著差异。分别为:女子篮球0.94±0.05、女子足球0.97±0.06、女子乒乓球0.93±0.04、女子羽毛球0.97±0.08。”;mso-font-kerning: 1.0000分;> + HR2 + hr3 - 200) / 10。其中,HR1、HR2、HR3分别表示运动员在平静状态下、刚结束运动时和恢复阶段的心率。本研究采用不同项目中女运动员的CRI和HRr值进行数据评估和统计分析。结论心储备是指心输出量根据机体代谢需要而增加的能力。当人们做一些剧烈运动时。交感神经会活跃,肾上腺素分泌会增加。同时,由于心率储备和脑力劳动的增加,心输出量也会增加。在运动和平静状态下,心率和SV等指标可以更全面地反映心脏的储存功能。心脏储备包括心率储备和心脏收缩能力储备。这项研究显示了女性的条件运动员的心脏储备通过使用超声心动描记法直接检测心输出量的变化前后人们运动型这种方法,我们可以更直接的结果.Considering女性运动员的大变化在青春期身高和体重,我们用心脏指数的变化来取代它的心输出量。通过这种方法,我们可以使评价指标更独特当人们做同样的事情的运动时,心率和心输出量增加越少的人心脏储备越大。因此,我们可以比较运动所做的功(PP)和心脏指数CI1、CI2的差值,得到心脏储备指数(CRI)。研究结果表明,不同项目的女运动员CRI平均值存在显著差异,可以得出CRI具有典型的项目特征的结论,因此可以将CRI作为反映女运动员项目特征的评价指标,也可以将CRI用于选拔不同项目的青年运动员。
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引用次数: 0
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Exercise Biochemistry Review
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