Wenqing Shen, Yi Zhang, Qiang Zhang, Zhengtang Qi, Yi Sun, Shuzhe Ding
Objective Type 2 diabetes mellitus is a common chronic diseases prevailing in the world and the amount of diabetic and pre-diabetic patients is increasing gradually. Exercise combined with hypoglycemic drug is the first recommended therapy to treat type 2 diabetes. Metformin was found from galegine in 1957 and has been used now as the first cheap and effective hypoglycemic guanidines. Our study aims to explore the effects of different ways of acute exercise intervention combined with high dose of metformin on glucose homeostasis and its relative molecular mechanisms in type 2 diabetic mice. Methods Adopt 4-week high fat diet (HFD, 45% fat content) and one-time STZ (Streptozocin, 100mg/kg) intraperitoneal injection to build type 2 diabetic mice. There are 84 mice in total, 24 mice were divided into three groups: normal control (NC) group, normal acute resistance training (NCR) group and normal acute endurance training (NCE) group, N=8 each group, they were fed normal chow. The rest 60 mice were fed HFD as T2D modeling group. 48 mice were developing type 2 diabetes and they were divided into 6 groups: diabetic control (DC) group, diabetic acute resistance training (DCR) group, diabetic acute endurance training (DCE) group, high dose of metformin control (HMC) group, high dose of metformin combined with acute resistance training (HMR) group and high dose of metformin combined with acute endurance training (HME) group, N=8 each group. Acute resistance training is climbing 1 meter ladder from down to up, 5 times a group, 3 groups in total, monitoring the glucose change with extracting mouse tail vein blood during each group, using ACCU-CHEK monitor. Acute endurance training is running at the speed of 18 m/min on the platform for 50 minutes and blood glucose change was monitored every 10 minutes by extracting mouse tail vein blood. HMC, HMR and HME group mice were intraperitoneally injected high dose of metformin (200mg/kg) one hour before the acute exercise intervention. Comparatively, NC, NCR, NCE, DC, DCR, DCE group mice were intraperitoneally injected 0.9% saline one hour before the acute exercise intervention. ELISA, RT-PCR and Western Blot were used to evaluate relative serum indicators, mRNA and protein expression of regulating blood glucose homeostasis. Results 1) 4-week high fat diet and one time 100mg/kg Streptozocin intraperitoneal injection induces mice to develop type 2 diabetes. The fasting blood glucose, IPGTT, ITT, glucose AUC and insulin AUC of T2D group mice are significantly higher than NC group. 2) Compared with DCR group, the blood glucose value and fluctuation of HMR group mice are both significantly decreased, but the blood glucose value of DCR and HMR group mice are significantly higher than NCR group. In the same way, the blood glucose value and fluctuation of HME group mice is lower than DCE group and the whole blood glucose level of both group are higher than NCE group. Acute resistance training and acute endurance training
{"title":"PO-062 Acute exercise intervention combined with metformin’s influences on glucose homeostasis in T2D mice","authors":"Wenqing Shen, Yi Zhang, Qiang Zhang, Zhengtang Qi, Yi Sun, Shuzhe Ding","doi":"10.14428/EBR.V1I3.10823","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.10823","url":null,"abstract":"Objective Type 2 diabetes mellitus is a common chronic diseases prevailing in the world and the amount of diabetic and pre-diabetic patients is increasing gradually. Exercise combined with hypoglycemic drug is the first recommended therapy to treat type 2 diabetes. Metformin was found from galegine in 1957 and has been used now as the first cheap and effective hypoglycemic guanidines. Our study aims to explore the effects of different ways of acute exercise intervention combined with high dose of metformin on glucose homeostasis and its relative molecular mechanisms in type 2 diabetic mice. \u0000Methods Adopt 4-week high fat diet (HFD, 45% fat content) and one-time STZ (Streptozocin, 100mg/kg) intraperitoneal injection to build type 2 diabetic mice. There are 84 mice in total, 24 mice were divided into three groups: normal control (NC) group, normal acute resistance training (NCR) group and normal acute endurance training (NCE) group, N=8 each group, they were fed normal chow. The rest 60 mice were fed HFD as T2D modeling group. 48 mice were developing type 2 diabetes and they were divided into 6 groups: diabetic control (DC) group, diabetic acute resistance training (DCR) group, diabetic acute endurance training (DCE) group, high dose of metformin control (HMC) group, high dose of metformin combined with acute resistance training (HMR) group and high dose of metformin combined with acute endurance training (HME) group, N=8 each group. Acute resistance training is climbing 1 meter ladder from down to up, 5 times a group, 3 groups in total, monitoring the glucose change with extracting mouse tail vein blood during each group, using ACCU-CHEK monitor. Acute endurance training is running at the speed of 18 m/min on the platform for 50 minutes and blood glucose change was monitored every 10 minutes by extracting mouse tail vein blood. HMC, HMR and HME group mice were intraperitoneally injected high dose of metformin (200mg/kg) one hour before the acute exercise intervention. Comparatively, NC, NCR, NCE, DC, DCR, DCE group mice were intraperitoneally injected 0.9% saline one hour before the acute exercise intervention. ELISA, RT-PCR and Western Blot were used to evaluate relative serum indicators, mRNA and protein expression of regulating blood glucose homeostasis. \u0000Results 1) 4-week high fat diet and one time 100mg/kg Streptozocin intraperitoneal injection induces mice to develop type 2 diabetes. The fasting blood glucose, IPGTT, ITT, glucose AUC and insulin AUC of T2D group mice are significantly higher than NC group. 2) Compared with DCR group, the blood glucose value and fluctuation of HMR group mice are both significantly decreased, but the blood glucose value of DCR and HMR group mice are significantly higher than NCR group. In the same way, the blood glucose value and fluctuation of HME group mice is lower than DCE group and the whole blood glucose level of both group are higher than NCE group. Acute resistance training and acute endurance training","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89489278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To investigate the mechanism of resveratrol improving iron overload in liver of mice and its effect on alleviating liver oxidative damage. Methods 40 male Balb/Cj mice, 2 months old, were randomly divided into 4 groups, Quiet control group (group C), sucrose iron group (group I), resveratrol group (group R), resveratrol + sucrose iron group (group IR), 10 in each group. Group IC and group IR were intraperitoneally injected with sucrose iron solution (100mg/5mL) once every two days, 75uL each time; R group and IR group were intragastrically resveratrol (Res), dissolved in 1% DMSO solution, each time 30mg / Kg; Group C and IC group were intragastrically administered with 1% DMSO solution once daily for 30 mg/Kg. After the end of the intervention for 8 weeks, the mice were dissected and taken. Perls' staining was used to observe the distribution of iron in the liver of mice; biochemical kit for serum glucose, serum iron (SI), total iron binding capacity (TIBC), liver iron, liver superoxide dismutase (T-SOD), liver malondialdehyde (MDA), total liver oxidative capacity (T-AOC); enzyme-linked immunosorbent assay (ELISA) for detection of serum ferritin (SF), octahydroxydeoxyguanosine (8-OHdG); Western blot detection of FPN1 protein expression in liver; the expression of Hepcidin in liver was detected by PCR. Results (1) Perls' staining results: 8 weeks of iron sucrose caused a significant increase in iron content in mouse hepatocytes, and Res decreased the amount of iron ions in hepatocytes; (2) Results of iron metabolism index: 8 weeks of sucrose iron had a significant increase in liver iron, SI, TIBC and SF (P<0.01), and Res intervention reduced liver iron (P<0.05) and SF (P<0.01). The content of TIBC (P<0.01) and SI (P<0.05) increased, and the iron supplement and Res interacted with the effects of liver iron, SI and TIBC; (3) Results of glucose metabolism index: iron overload increased glucose level in mice (P<0.01), Res decreased glucose level, iron overload reduced liver glycogen storage in mice (P<0.05), iron supplement and Res, there is no interaction on the effects of glucose metabolism indicators; (4) Oxidation index results: 8 weeks of sucrose iron significantly inhibited liver T-SOD and T-AOC activity (P<0.01), increased liver MDA and 8-OHDG activity (P<0.01), and Res promoted liver T-AOC (P<0.01) and T-SOD activity increased, and the liver 8-OHDG (P<0.01) and liver MDA activity were decreased. The effects of iron supplement and Res on 8-OHDG and T-AOC were interactive (P<0.01); (5) 8 weeks of sucrose iron inhibited the expression of FPN1 protein in the liver of mice, and the intervention of Res could enhance the expression of FPN1 protein; iron supplement promoted the expression of Hepcidin in liver (P<0.01), Res inhibited Hepcidin, iron supplement and Res had no interaction with the expression of FPN1 and Hepcidin in liver. Conclusions 8 weeks of sucrose iron caused iron overload in the liver of mice, and increased oxidative st
{"title":"PO-295 Effect of resveratrol on preventing iron overload in liver of mice","authors":"Lan-ying Li","doi":"10.14428/EBR.V1I5.13303","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.13303","url":null,"abstract":"Objective To investigate the mechanism of resveratrol improving iron overload in liver of mice and its effect on alleviating liver oxidative damage. \u0000Methods 40 male Balb/Cj mice, 2 months old, were randomly divided into 4 groups, Quiet control group (group C), sucrose iron group (group I), resveratrol group (group R), resveratrol + sucrose iron group (group IR), 10 in each group. Group IC and group IR were intraperitoneally injected with sucrose iron solution (100mg/5mL) once every two days, 75uL each time; R group and IR group were intragastrically resveratrol (Res), dissolved in 1% DMSO solution, each time 30mg / Kg; Group C and IC group were intragastrically administered with 1% DMSO solution once daily for 30 mg/Kg. After the end of the intervention for 8 weeks, the mice were dissected and taken. Perls' staining was used to observe the distribution of iron in the liver of mice; biochemical kit for serum glucose, serum iron (SI), total iron binding capacity (TIBC), liver iron, liver superoxide dismutase (T-SOD), liver malondialdehyde (MDA), total liver oxidative capacity (T-AOC); enzyme-linked immunosorbent assay (ELISA) for detection of serum ferritin (SF), octahydroxydeoxyguanosine (8-OHdG); Western blot detection of FPN1 protein expression in liver; the expression of Hepcidin in liver was detected by PCR. \u0000Results (1) Perls' staining results: 8 weeks of iron sucrose caused a significant increase in iron content in mouse hepatocytes, and Res decreased the amount of iron ions in hepatocytes; (2) Results of iron metabolism index: 8 weeks of sucrose iron had a significant increase in liver iron, SI, TIBC and SF (P<0.01), and Res intervention reduced liver iron (P<0.05) and SF (P<0.01). The content of TIBC (P<0.01) and SI (P<0.05) increased, and the iron supplement and Res interacted with the effects of liver iron, SI and TIBC; (3) Results of glucose metabolism index: iron overload increased glucose level in mice (P<0.01), Res decreased glucose level, iron overload reduced liver glycogen storage in mice (P<0.05), iron supplement and Res, there is no interaction on the effects of glucose metabolism indicators; (4) Oxidation index results: 8 weeks of sucrose iron significantly inhibited liver T-SOD and T-AOC activity (P<0.01), increased liver MDA and 8-OHDG activity (P<0.01), and Res promoted liver T-AOC (P<0.01) and T-SOD activity increased, and the liver 8-OHDG (P<0.01) and liver MDA activity were decreased. The effects of iron supplement and Res on 8-OHDG and T-AOC were interactive (P<0.01); (5) 8 weeks of sucrose iron inhibited the expression of FPN1 protein in the liver of mice, and the intervention of Res could enhance the expression of FPN1 protein; iron supplement promoted the expression of Hepcidin in liver (P<0.01), Res inhibited Hepcidin, iron supplement and Res had no interaction with the expression of FPN1 and Hepcidin in liver. \u0000Conclusions 8 weeks of sucrose iron caused iron overload in the liver of mice, and increased oxidative st","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88754131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Human muscle consists of a mixture of fibres with different contractile and metabolic properties, type I (slow-twitch) and type II (fast-twitch) fibres. Little is known about the effect of anabolic stimuli, in particular nutrition, on the molecular response in the different fibre types. Here, we examine the effect of resistance exercise and essential amino acid (EAA) supplementation on mTOR signalling in individual type I and type II human muscle fibres. Methods Five strength-trained male subjects performed two sessions of leg press exercise (10 x 10 repetition at 62-85 % of 1RM). During exercise and recovery, the subjects ingested an aqueous solution with EAA (290 mg/kg) or flavoured water (placebo). Muscle biopsies were taken from the vastus lateralis before and 90 min after exercise. The biopsies were freeze-dried and single fibres dissected out and weighed (range 0.9 – 8 ug). The fibres were individually homogenized and analysed for proteins in the mTOR pathway using Western blot. Membranes were repeatedly stripped and fibres were identified as type I or type II following incubation with antibodies against the different myosin isoforms. Results Exercise led to a significant increase in mTOR and p70S6k1 phosphorylation and a fall in eEF2 phosphorylation, similar in both fibre types. There was a large variation between individual fibres; some fibres were highly activated whereas others were not activated at all despite the heavy exercise performed. Intake of EAA caused a 2- to 6-fold higher increase in mTOR and p70S6k1 phosphorylation in both type I and type II fibres as compared to intake of placebo, with no difference between the fibre types. The phosphorylation of eEF2 was not affected by intake of EAA. The total expression of p70S6k1 and eEF2 was 145% and 155% higher in type II than in type I fibres (P<0.05), respectively, whereas no difference between the fibre types was observed for mTOR protein. Conclusions The response to heavy resistance exercise regarding mTOR signalling was similar in type I and type II fibres in trained subjects, but with a large variation between single fibres of both types. Furthermore, ingestion of EAA enhanced the effect of resistance exercise on phosphorylation of mTOR and p70S6k1 in both fibre types.
{"title":"PO-260 Anabolic signalling in individual muscle fibres following resistance exercise in combination with amino acid intake","authors":"S. Edman, K. Söderlund, E. Blomstrand","doi":"10.14428/EBR.V1I5.11083","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.11083","url":null,"abstract":"Objective Human muscle consists of a mixture of fibres with different contractile and metabolic properties, type I (slow-twitch) and type II (fast-twitch) fibres. Little is known about the effect of anabolic stimuli, in particular nutrition, on the molecular response in the different fibre types. Here, we examine the effect of resistance exercise and essential amino acid (EAA) supplementation on mTOR signalling in individual type I and type II human muscle fibres. \u0000Methods Five strength-trained male subjects performed two sessions of leg press exercise (10 x 10 repetition at 62-85 % of 1RM). During exercise and recovery, the subjects ingested an aqueous solution with EAA (290 mg/kg) or flavoured water (placebo). Muscle biopsies were taken from the vastus lateralis before and 90 min after exercise. The biopsies were freeze-dried and single fibres dissected out and weighed (range 0.9 – 8 ug). The fibres were individually homogenized and analysed for proteins in the mTOR pathway using Western blot. Membranes were repeatedly stripped and fibres were identified as type I or type II following incubation with antibodies against the different myosin isoforms. \u0000Results Exercise led to a significant increase in mTOR and p70S6k1 phosphorylation and a fall in eEF2 phosphorylation, similar in both fibre types. There was a large variation between individual fibres; some fibres were highly activated whereas others were not activated at all despite the heavy exercise performed. Intake of EAA caused a 2- to 6-fold higher increase in mTOR and p70S6k1 phosphorylation in both type I and type II fibres as compared to intake of placebo, with no difference between the fibre types. The phosphorylation of eEF2 was not affected by intake of EAA. The total expression of p70S6k1 and eEF2 was 145% and 155% higher in type II than in type I fibres (P<0.05), respectively, whereas no difference between the fibre types was observed for mTOR protein. \u0000Conclusions The response to heavy resistance exercise regarding mTOR signalling was similar in type I and type II fibres in trained subjects, but with a large variation between single fibres of both types. Furthermore, ingestion of EAA enhanced the effect of resistance exercise on phosphorylation of mTOR and p70S6k1 in both fibre types. \u0000 ","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89361976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Low-load blood flow restriction training (It's also called KAATSU) refers to the stimulation of skeletal muscle growth and muscle strength with low-load training intensity when the blood flow of the proximal venous vessels of the limbs is restricted for a short time. Thereby improving the muscle function of the skeletal muscle. KAATSU uses a low training load (20% IRM), which can play a medium to high load training effect. It is suitable for older people with weaker constitution, less skeletal muscle strength, and different degrees of aging muscle loss (sarcopenia). Therefore, there have been more and more studies on KAATSU intervention in chronic diseases in the elderly in recent years. This study systematically reviews the recent progress in the application of KAATSU in chronic diseases in the elderly, and provides a theoretical basis for the widespread application of KAATSU in the intervention of chronic diseases in the elderly. Methods Enter keywords in the websites of CNKI, CQVIP, Elsevier Science Direct, and Journals @Ovid Full Text, Pub Met, etc: “KAATSU, chronic disease”/“blood flow restriction, older”, a total of more than 3000 articles were searched, Screening of 98, At last, more than 40 literatures meeting the standards were analyzed and summarized. Results 1) Applied to skeletal muscle atrophy: KAATSU can significantly increase the cross-sectional area of skeletal muscle muscles, strengthen muscle strength, improve muscle contraction function, effectively prevent and interfere with sarcopenia, Especially for stroke patients with rehabilitation and long-term bed caused by muscular atrophy of disuse has a good clinical effect.2) Application to osteoporosis: KAATSU can improve bone mineral density, stimulate bone cell activity and improve bone health, but it is not yet certain whether to improve Senile osteoporosis. Future research should be combined with nutritional supplements to comprehensively evaluate its therapeutic effect. 3) Applied to cardiovascular and cerebrovascular diseases: Long-term KAATSU can effectively improve blood lipid metabolism, optimize cardiovascular function parameters, It doesn't activate the thrombin system, and has no significant negative impact on cardiovascular function in the elderly, the application effect is good in cardiovascular and cerebrovascular diseases such as atherosclerosis and hypertension.4) Application to metabolic syndrome: KAATSU can promote fat breakdown, reduce BMI,body fat percentage, insulin resistance index, glycosylated hemoglobin index, and improve diabetes and senile obesity in the elderly. 5) Others: There are a few studies showing that KAATSU can improve the daily activities of patients with Alzheimer's disease, promote the remodeling of bone tissue in elderly patients with osteonecrosis, and intervene with the muscle atrophy of patients with certain chronic heart diseases. Conclusions KAATSU is an effective exercise method for the elderly and has a good applica
{"title":"PO-287 Application of low-load blood flow restriction training in elderly chronic diseases","authors":"Jinna Cheng, Zhiguang Zhao","doi":"10.14428/EBR.V1I5.13123","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.13123","url":null,"abstract":"Objective Low-load blood flow restriction training (It's also called KAATSU) refers to the stimulation of skeletal muscle growth and muscle strength with low-load training intensity when the blood flow of the proximal venous vessels of the limbs is restricted for a short time. Thereby improving the muscle function of the skeletal muscle. KAATSU uses a low training load (20% IRM), which can play a medium to high load training effect. It is suitable for older people with weaker constitution, less skeletal muscle strength, and different degrees of aging muscle loss (sarcopenia). Therefore, there have been more and more studies on KAATSU intervention in chronic diseases in the elderly in recent years. This study systematically reviews the recent progress in the application of KAATSU in chronic diseases in the elderly, and provides a theoretical basis for the widespread application of KAATSU in the intervention of chronic diseases in the elderly. \u0000Methods Enter keywords in the websites of CNKI, CQVIP, Elsevier Science Direct, and Journals @Ovid Full Text, Pub Met, etc: “KAATSU, chronic disease”/“blood flow restriction, older”, a total of more than 3000 articles were searched, Screening of 98, At last, more than 40 literatures meeting the standards were analyzed and summarized. \u0000Results 1) Applied to skeletal muscle atrophy: KAATSU can significantly increase the cross-sectional area of skeletal muscle muscles, strengthen muscle strength, improve muscle contraction function, effectively prevent and interfere with sarcopenia, Especially for stroke patients with rehabilitation and long-term bed caused by muscular atrophy of disuse has a good clinical effect.2) Application to osteoporosis: KAATSU can improve bone mineral density, stimulate bone cell activity and improve bone health, but it is not yet certain whether to improve Senile osteoporosis. Future research should be combined with nutritional supplements to comprehensively evaluate its therapeutic effect. 3) Applied to cardiovascular and cerebrovascular diseases: Long-term KAATSU can effectively improve blood lipid metabolism, optimize cardiovascular function parameters, It doesn't activate the thrombin system, and has no significant negative impact on cardiovascular function in the elderly, the application effect is good in cardiovascular and cerebrovascular diseases such as atherosclerosis and hypertension.4) Application to metabolic syndrome: KAATSU can promote fat breakdown, reduce BMI,body fat percentage, insulin resistance index, glycosylated hemoglobin index, and improve diabetes and senile obesity in the elderly. 5) Others: There are a few studies showing that KAATSU can improve the daily activities of patients with Alzheimer's disease, promote the remodeling of bone tissue in elderly patients with osteonecrosis, and intervene with the muscle atrophy of patients with certain chronic heart diseases. \u0000Conclusions KAATSU is an effective exercise method for the elderly and has a good applica","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89285896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To investigate the effects of long-term various load aerobic exercise on the free radical metabolism of the brain in aging mice through the establishment of different swimming exercise models. Methods sixty 3-month-old male Kunming mice were randomly and equally divided into five groups, which were the control group (group C), 10 minute exercise group (group E1), 30 minute exercise group (group E2), 60 minute exercise group (group E3), and 90 minute exercise group (group E4). Group C had regular feeding during the natural aging time in 8 months without any exercise intervention. All other exercise groups performed different load swimming exercise (from 10 to 90 minutes as mentioned above) during the same 8 month period. All subjects were decapitated after exercise on the last day of the 8th month; and the brain tissues were harvested as samples. The malondialdehyde (MDA) amount was measured by the Thiobarbituric Acid method, and the superoxide dismutase (SOD) activity level was measured with Xanthine Oxidase method. Results group E3 demonstrated significantly higher SOD activity level and lower MDA amount than the other groups. Conclusions Long-term, medium-load aerobic exercise had greatly impacted the metabolism of free radicals in the brain of the mouse, evidenced by increased SOD activity level and decreased MDA production. These effects may indicate that this exercise model may be beneficial in slowing down brain aging by eliminating free radicals and improving brain anti-oxidation ability.
{"title":"PO-113 Long-term Various Load Training Effects on Free Radical Metabolism in the Brain of Aging Mice","authors":"Cuilan Wei, Xue Li","doi":"10.14428/EBR.V1I4.9813","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.9813","url":null,"abstract":"Objective To investigate the effects of long-term various load aerobic exercise on the free radical metabolism of the brain in aging mice through the establishment of different swimming exercise models. \u0000Methods sixty 3-month-old male Kunming mice were randomly and equally divided into five groups, which were the control group (group C), 10 minute exercise group (group E1), 30 minute exercise group (group E2), 60 minute exercise group (group E3), and 90 minute exercise group (group E4). Group C had regular feeding during the natural aging time in 8 months without any exercise intervention. All other exercise groups performed different load swimming exercise (from 10 to 90 minutes as mentioned above) during the same 8 month period. All subjects were decapitated after exercise on the last day of the 8th month; and the brain tissues were harvested as samples. The malondialdehyde (MDA) amount was measured by the Thiobarbituric Acid method, and the superoxide dismutase (SOD) activity level was measured with Xanthine Oxidase method. \u0000Results group E3 demonstrated significantly higher SOD activity level and lower MDA amount than the other groups. \u0000Conclusions Long-term, medium-load aerobic exercise had greatly impacted the metabolism of free radicals in the brain of the mouse, evidenced by increased SOD activity level and decreased MDA production. These effects may indicate that this exercise model may be beneficial in slowing down brain aging by eliminating free radicals and improving brain anti-oxidation ability.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89921011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Through testing and analysis the characteristics of body shape，body composition，bone growth and physical fitness，hemoglobin, testosterone of 6-7 years old female preselected tennis players，the study aim was to provide reference bases for the early selection of female tennis players. Methods A total of 75 female preselected tennis players(initial selection by the coaches) aged from 6 to 7 years were came from Hebei, Hubei, Qinghai and Inner Mongolia province, who came to Research Center for Heath related Physical Fitness Evaluation of Guangzhou Sport University for physical fitness test from July 2016 to July 2018. The height, weight, length of upper limbs, length of lower limbs, iliac width, shoulder width, body fat, muscle mass, bone age，bone mass density(BMD), anaerobic power and PWC170, reaction time, vertical jump, grip strength, hemoglobin, testosterone were measured using related instruments and methods, and calculated derived indicators BMI, iliac width/shoulder width. Data were compared with the national standard of physical health of students and/or evaluated by deviation method，and correlation had been analysed among physical parameters. Results 1)The 75 female preselected tennis players’ aged from 6 to 7 years height and weight were 128.10±5.32cm and 25.70±3.87kg，and there are 47 girls height upper medium grade level, 60% of which weight was at a moderate level, their BMI were 15.48±1.50kg/m2，and all in the normal range, iliac width/shoulder width ×100 was 76.52±7.00, 70.7% 0f which was above medium grade level, the upper and lower limbs were 54.28±3.60cm and 71.68±5.26cm, girls’ PBF were 21.03±6.44, muscle weight were 18.94±3.00kg, BMD were 2.04±2.20, and no low bone strength were fund; Anaerobic power of all female preselected tennis players were 135.93±31.65kg.cm, and the values of the PWC170 relative weight were 10.79±2.56kg.m/min.kg, reaction time were 0.628±0.128s, vertical jump were 21.13±4.95cm, the grip of right and left hand were 10.36±2.15kg and 10.06±2.40kg, the physical fitness parameters above in the upper middle class were more girls than the lower middle class; The hemoglobin content was 132.15±8.70g/L, which was above the normal level (110 -160g/L), the serum testosterone concentration was 1.52±1.20umol/L, which was much higher than that of normal girls (0-0.7umol/L). 2) When age was controlled, there was negative correlation between T and PFB, vertical jump and body weight, PWC170 and reaction time(P＜0.05), and there was positive correlation between hemoglobin and muscle weight(P＜0.05), height, and vertical jump(P＜0.01), muscle weight and anaerobic power(P<0.01)，anaerobic power and height，weight，BMI，upper and lower limbs(P<0.01, P<0.01, P<0.01, P<0.05, P<0.01)，PWC170 and vertical jump，the grip of right and left hand(P<0.01, P<0.05, P<0.05), vertical jump and upper, lower limbs, iliac width/shoulder width(P<0.01, P<0.01, P<0.01). Conclusions 75 female aged from 6 to 7 years old preselected tennis players’
{"title":"OR-023 Physical evaluation of 6-7 years old female preselected tennis players","authors":"Xiquan Weng, Chaoge Wang, Yan Meng, Guoqin Xu","doi":"10.14428/EBR.V1I2.9243","DOIUrl":"https://doi.org/10.14428/EBR.V1I2.9243","url":null,"abstract":"Objective Through testing and analysis the characteristics of body shape，body composition，bone growth and physical fitness，hemoglobin, testosterone of 6-7 years old female preselected tennis players，the study aim was to provide reference bases for the early selection of female tennis players. \u0000Methods A total of 75 female preselected tennis players(initial selection by the coaches) aged from 6 to 7 years were came from Hebei, Hubei, Qinghai and Inner Mongolia province, who came to Research Center for Heath related Physical Fitness Evaluation of Guangzhou Sport University for physical fitness test from July 2016 to July 2018. The height, weight, length of upper limbs, length of lower limbs, iliac width, shoulder width, body fat, muscle mass, bone age，bone mass density(BMD), anaerobic power and PWC170, reaction time, vertical jump, grip strength, hemoglobin, testosterone were measured using related instruments and methods, and calculated derived indicators BMI, iliac width/shoulder width. Data were compared with the national standard of physical health of students and/or evaluated by deviation method，and correlation had been analysed among physical parameters. \u0000Results 1)The 75 female preselected tennis players’ aged from 6 to 7 years height and weight were 128.10±5.32cm and 25.70±3.87kg，and there are 47 girls height upper medium grade level, 60% of which weight was at a moderate level, their BMI were 15.48±1.50kg/m2，and all in the normal range, iliac width/shoulder width ×100 was 76.52±7.00, 70.7% 0f which was above medium grade level, the upper and lower limbs were 54.28±3.60cm and 71.68±5.26cm, girls’ PBF were 21.03±6.44, muscle weight were 18.94±3.00kg, BMD were 2.04±2.20, and no low bone strength were fund; Anaerobic power of all female preselected tennis players were 135.93±31.65kg.cm, and the values of the PWC170 relative weight were 10.79±2.56kg.m/min.kg, reaction time were 0.628±0.128s, vertical jump were 21.13±4.95cm, the grip of right and left hand were 10.36±2.15kg and 10.06±2.40kg, the physical fitness parameters above in the upper middle class were more girls than the lower middle class; The hemoglobin content was 132.15±8.70g/L, which was above the normal level (110 -160g/L), the serum testosterone concentration was 1.52±1.20umol/L, which was much higher than that of normal girls (0-0.7umol/L). \u00002) When age was controlled, there was negative correlation between T and PFB, vertical jump and body weight, PWC170 and reaction time(P＜0.05), and there was positive correlation between hemoglobin and muscle weight(P＜0.05), height, and vertical jump(P＜0.01), muscle weight and anaerobic power(P<0.01)，anaerobic power and height，weight，BMI，upper and lower limbs(P<0.01, P<0.01, P<0.01, P<0.05, P<0.01)，PWC170 and vertical jump，the grip of right and left hand(P<0.01, P<0.05, P<0.05), vertical jump and upper, lower limbs, iliac width/shoulder width(P<0.01, P<0.01, P<0.01). \u0000Conclusions 75 female aged from 6 to 7 years old preselected tennis players’","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90240726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective atherosclerosis has been associated with the progression of cognitive impairment and dementia. Several features, such as high oxygen consumption, a large content of peroxidation-sensitive polyunsaturated fatty acids (PUFAs) and a strong dependency on the supply of glucose make the brain vulnerable to even small metabolic changes. The hippocampus is closely related to memory and learning function, and prone to ischemic injury. However, using metabolomics technology to explore metabolites of hippocampus from atherosclerosis animals is rarely reported. We aim to reveal the metabolic changes during atherosclerosis, and clarify the possible role of exercise in regulating hippocampus metabolism. Methods we established a rat model of atherosclerosis(n=18) along with control group (n=10). The model group was assigned into the AS group (n=8) and the TAS group (n=8), which was intervened by running exercise for 4 weeks. A Y maze test was performed to evaluate initial memory. Metabolomics based on GC-MS was applied to detect small molecules metabolites in rat hippocampus. Results we found that the AS and TAS group both showed elevation in HDL, meanwhile decrement in TC and LDL after 4 weeks’ intervention. The behavioral test showed rats from AS group entered less frequently into and spent less time in the novel arm than rats from C group (P<0.01), while other indexes showed no difference. Compared to the C group, metabolites including xylulose 5-phosphate, threonine, succinate and nonanoic acid were markedly elevated, whereas methyl arachidonic acid and methyl stearate decreased in the AS group. Meanwhile, the levels of succinic acid, branched chain amino acids, nonanoic acid and desmosterol decreased, whereas methyl arachidonic acid, methyl stearate, and glyceraldehyde-3-phosphate elevated in the hippocampus of the TAS group in comparison with the AS group. Conclusions A series of metabolic changes implicated in the hippocampus of atherosclerotic rats, including a decrease in anaerobic glycolysis and TCA cycle, an activation of pentose phosphate pathway, and a disturbance in fatty acid oxidation and cholesterol synthesis, which could lead to insufficient ATP in the hippocampus and related to the behavioral changes of atherosclerotic rats, while running exercise may take part in regulating metabolism to normal state in the hippocampus of atherosclerotic rats.
{"title":"PO-153 The metabolic changes in the hippocampus of an atherosclerotic rat model and the regulation of exercise","authors":"B. Liu, Shujie Lou","doi":"10.14428/EBR.V1I4.11773","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.11773","url":null,"abstract":"Objective atherosclerosis has been associated with the progression of cognitive impairment and dementia. Several features, such as high oxygen consumption, a large content of peroxidation-sensitive polyunsaturated fatty acids (PUFAs) and a strong dependency on the supply of glucose make the brain vulnerable to even small metabolic changes. The hippocampus is closely related to memory and learning function, and prone to ischemic injury. However, using metabolomics technology to explore metabolites of hippocampus from atherosclerosis animals is rarely reported. We aim to reveal the metabolic changes during atherosclerosis, and clarify the possible role of exercise in regulating hippocampus metabolism. \u0000Methods we established a rat model of atherosclerosis(n=18) along with control group (n=10). The model group was assigned into the AS group (n=8) and the TAS group (n=8), which was intervened by running exercise for 4 weeks. A Y maze test was performed to evaluate initial memory. Metabolomics based on GC-MS was applied to detect small molecules metabolites in rat hippocampus. \u0000Results we found that the AS and TAS group both showed elevation in HDL, meanwhile decrement in TC and LDL after 4 weeks’ intervention. The behavioral test showed rats from AS group entered less frequently into and spent less time in the novel arm than rats from C group (P<0.01), while other indexes showed no difference. Compared to the C group, metabolites including xylulose 5-phosphate, threonine, succinate and nonanoic acid were markedly elevated, whereas methyl arachidonic acid and methyl stearate decreased in the AS group. Meanwhile, the levels of succinic acid, branched chain amino acids, nonanoic acid and desmosterol decreased, whereas methyl arachidonic acid, methyl stearate, and glyceraldehyde-3-phosphate elevated in the hippocampus of the TAS group in comparison with the AS group. \u0000Conclusions A series of metabolic changes implicated in the hippocampus of atherosclerotic rats, including a decrease in anaerobic glycolysis and TCA cycle, an activation of pentose phosphate pathway, and a disturbance in fatty acid oxidation and cholesterol synthesis, which could lead to insufficient ATP in the hippocampus and related to the behavioral changes of atherosclerotic rats, while running exercise may take part in regulating metabolism to normal state in the hippocampus of atherosclerotic rats.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90326432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Peroxisome proliferator-activated receptor-γ coactivator-1β (PGC-1β) is mainly expressed in mitochondria-rich tissues, which involved in skeletal muscle mitochondrial biosynthesis and energy metabolism processes such as fatty acid transport and oxidation, hepatic gluconeogenesis. PGC-1β is Previous studies have shown that this genetic polymorphism is associated with the athletic ability of elite endurance athletes. Therefore, based on the previous research, the relationship between PGC-1β gene polymorphism and aerobic exercise ability of elite female long-distance runners was discussed to provide new effective indicators for athletes' selection of materials, and improve the accuracy and advancement of athletes' selection of materials. Methods 56 Chinese elite female long-distance runners were selected, and venous blood was extracted to analyze the gene polymorphism of specific gene locus. The subjects were tested for aerobic endurance index and lung function index, wherein the aerobic endurance index included maximum oxygen uptake, relative maximal oxygen uptake relative value, anaerobic threshold and anaerobic threshold relative value, and lung function indicators including vital capacity, Time lung capacity, minute ventilation and maximum ventilation . Subsequently, the cross-sectional association study method was used to analyze the association between four genotypes locus of PGC-1β including rs32579, rs2161257, rs1544744 and rs10783180 in 56 subjects. Results 1) All four polymorphic locus were tested by H-W balance, indicating that the subjects were representative of the population. 2) rs32579 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 3) rs2161257 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 4) rs1544744 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 5) rs10783180 locus: There is a significant difference in the relative values of anaerobic threshold and anaerobic threshold between different genotype athletes. The anaerobic threshold of AG genotype athletes was 2156.35±227.69 ml/min and the anaerobic threshold of athletes with GG genotype was 2143.41±217.30 ml/min. So the anaerobic threshold of AG genotype athletes was significantly greater than the anaerobic threshold of GG genotype athletes. The Anaerobic threshold relative value for AG genotype athletes was 50.99±3.99 ml/kg/min, while the anaerobic threshold relative value for athletes with GG genotype was 48.12±4.25 ml/kg/min. The anaerobic threshold relative value of AG genotype athletes was significantly greater than that of GG genotype athletes Other indicators showed no significant difference. Conclusions The rs10783180 polymorphism is associated with the athletic ability of elite endurance
{"title":"OR-025 Association study between Chinese excellent long-distance female athletes’ PGC-1β genetic polymorphism and aerobic capacity","authors":"J. Jia, Chunyan Xu","doi":"10.14428/EBR.V1I2.9263","DOIUrl":"https://doi.org/10.14428/EBR.V1I2.9263","url":null,"abstract":"Objective Peroxisome proliferator-activated receptor-γ coactivator-1β (PGC-1β) is mainly expressed in mitochondria-rich tissues, which involved in skeletal muscle mitochondrial biosynthesis and energy metabolism processes such as fatty acid transport and oxidation, hepatic gluconeogenesis. PGC-1β is Previous studies have shown that this genetic polymorphism is associated with the athletic ability of elite endurance athletes. Therefore, based on the previous research, the relationship between PGC-1β gene polymorphism and aerobic exercise ability of elite female long-distance runners was discussed to provide new effective indicators for athletes' selection of materials, and improve the accuracy and advancement of athletes' selection of materials. \u0000Methods 56 Chinese elite female long-distance runners were selected, and venous blood was extracted to analyze the gene polymorphism of specific gene locus. The subjects were tested for aerobic endurance index and lung function index, wherein the aerobic endurance index included maximum oxygen uptake, relative maximal oxygen uptake relative value, anaerobic threshold and anaerobic threshold relative value, and lung function indicators including vital capacity, Time lung capacity, minute ventilation and maximum ventilation . Subsequently, the cross-sectional association study method was used to analyze the association between four genotypes locus of PGC-1β including rs32579, rs2161257, rs1544744 and rs10783180 in 56 subjects. \u0000Results 1) All four polymorphic locus were tested by H-W balance, indicating that the subjects were representative of the population. 2) rs32579 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 3) rs2161257 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 4) rs1544744 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 5) rs10783180 locus: There is a significant difference in the relative values of anaerobic threshold and anaerobic threshold between different genotype athletes. The anaerobic threshold of AG genotype athletes was 2156.35±227.69 ml/min and the anaerobic threshold of athletes with GG genotype was 2143.41±217.30 ml/min. So the anaerobic threshold of AG genotype athletes was significantly greater than the anaerobic threshold of GG genotype athletes. The Anaerobic threshold relative value for AG genotype athletes was 50.99±3.99 ml/kg/min, while the anaerobic threshold relative value for athletes with GG genotype was 48.12±4.25 ml/kg/min. The anaerobic threshold relative value of AG genotype athletes was significantly greater than that of GG genotype athletes \u0000Other indicators showed no significant difference. \u0000Conclusions The rs10783180 polymorphism is associated with the athletic ability of elite endurance ","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88042485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective The purpose was to further improve the understanding of exercise to improve dyslipidemia and to formulate exercise prescription more scientifically. It provides a reference for the further study of exercise mimics in the treatment of dyslipidemia and helps to reduce the high incidence of dyslipidemia. Methods Methods of documentation and comparative analysis are applied. Results It was found that the effects of exercise on dyslipidemia were beneficial or had no obvious effect on some indexes so far, and had no adverse effect. The biological mechanism of exercise regulating dyslipidemia and the effects of different exercise forms (acute exercise, long-term exercise training, different intensity exercise) on improving dyslipidemia were summarized. It is pointed out that exercise plays an important role in regulating the enzymes and proteins associated with dyslipidemia. Research from several aspects :exercise to block cholesterol biosynthesis, exercise inhibits cholesterol absorption, exercise affects cholesterol conversion, exercise promotes cholesterol conversion to bile acid, and exercise promotes cholesterol metabolism, exercise regulating triglycerides, etc. Conclusions The regulation of dyslipidemia is a complex process, involving multiple pathways, multiple gene regulation, and different hypolipidemic pathways.A large number of experimental studies have demonstrated the effect of exercise on the improvement of dyslipidemia, but there are few studies on the biological mechanism of exercise, which need to be further studied.In addition, when chemically synthesized anti-hyperlipidemia drugs have many safety problems, we should increase the in-depth study of sports drugs, especially some natural products, that can simulate exercise effectiveness. In order to better control the high incidence of dyslipidemia, it is necessary to improve the development of exercise mimic drugs in improving dyslipidemia.
{"title":"PO-130 Biological Mechanism of Exercise in Improving Dyslipidemia","authors":"Mi Tang, Q. Su","doi":"10.14428/EBR.V1I4.10323","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.10323","url":null,"abstract":"Objective The purpose was to further improve the understanding of exercise to improve dyslipidemia and to formulate exercise prescription more scientifically. It provides a reference for the further study of exercise mimics in the treatment of dyslipidemia and helps to reduce the high incidence of dyslipidemia. \u0000Methods Methods of documentation and comparative analysis are applied. \u0000Results It was found that the effects of exercise on dyslipidemia were beneficial or had no obvious effect on some indexes so far, and had no adverse effect. The biological mechanism of exercise regulating dyslipidemia and the effects of different exercise forms (acute exercise, long-term exercise training, different intensity exercise) on improving dyslipidemia were summarized. It is pointed out that exercise plays an important role in regulating the enzymes and proteins associated with dyslipidemia. Research from several aspects :exercise to block cholesterol biosynthesis, exercise inhibits cholesterol absorption, exercise affects cholesterol conversion, exercise promotes cholesterol conversion to bile acid, and exercise promotes cholesterol metabolism, exercise regulating triglycerides, etc. \u0000Conclusions The regulation of dyslipidemia is a complex process, involving multiple pathways, multiple gene regulation, and different hypolipidemic pathways.A large number of experimental studies have demonstrated the effect of exercise on the improvement of dyslipidemia, but there are few studies on the biological mechanism of exercise, which need to be further studied.In addition, when chemically synthesized anti-hyperlipidemia drugs have many safety problems, we should increase the in-depth study of sports drugs, especially some natural products, that can simulate exercise effectiveness. In order to better control the high incidence of dyslipidemia, it is necessary to improve the development of exercise mimic drugs in improving dyslipidemia.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89818174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Intermittent exercise can improve cardiac function in rats with myocardial infarction. The Neuregulin-1(NRG1)/SERCA2a palys a critical role in maintain cardiac function. We want to investigate the effect of Neuregulin-1 (NRG1) on NRG1-SERCA2a signaling pathway activated by intermittent exercise and on improves cardiac function in rats with MI. Methods 32 male sprague-dawley rats were randomly divided into four groups (n=8): Sham-operated group (S), sedentary MI group (MI), MI with interval training group (ME), ME with inhibitor AG1478 group (MA). ME and MA model after the MI model was established by ligation of the left anterior descending coronary artery, and began training 1 week after MI surgery. The S model only by threading without ligation. Rats in ME and MA model taken one week adaptive training, then began 8-week interval training. MA model were injected with inhibitor AG1478, once every two days. The 24h after training, rats were anesthetized, the LVSP, LVEDP, ±dp/dt max were tested by carotid artery intubation which in order to evaluate cardiac function. The protein expression of NRG1, PI3K, Akt, eNOS, PKG, PLN, SERCA2a in myocardium were measured by Westernblotting, themRNA expression of serca2a were tested by RT-qPCR. Results Compared with S, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a decreased, serca2a mRNA expression decreased, LVSP and ±dp/dt max significantly decreased, LVEDP significantly increased; Compared with MI, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a increased, serca2a mRNA expression increased, LVSP and ±dp/dt max significantly increased, LVEDP decreased, and the effect of exercise were weaken by inhibitor AG1478. Correlation analysis showed that the myocardial pPLN and SERCA2a protein expression both were positively correlated with LVSP, ±dp/dtmax, and negatively correlated with LVEDP. Conclusions Intermittent exercise can increased myocardial NRG1 protein expression and activates NRG1-SERCA2a signaling pathway, improve myocardial infarction cardiac function.
{"title":"PO-144 Intermittent Exercise Activates NRG1-SERCA2a Pathway to Improve Cardiac Function in Myocardial Infarction Rats","authors":"Wenyan Bo, Dagang Li, Zhenjun Tian","doi":"10.14428/EBR.V1I4.10473","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.10473","url":null,"abstract":"Objective Intermittent exercise can improve cardiac function in rats with myocardial infarction. The Neuregulin-1(NRG1)/SERCA2a palys a critical role in maintain cardiac function. We want to investigate the effect of Neuregulin-1 (NRG1) on NRG1-SERCA2a signaling pathway activated by intermittent exercise and on improves cardiac function in rats with MI. \u0000Methods 32 male sprague-dawley rats were randomly divided into four groups (n=8): Sham-operated group (S), sedentary MI group (MI), MI with interval training group (ME), ME with inhibitor AG1478 group (MA). ME and MA model after the MI model was established by ligation of the left anterior descending coronary artery, and began training 1 week after MI surgery. The S model only by threading without ligation. Rats in ME and MA model taken one week adaptive training, then began 8-week interval training. MA model were injected with inhibitor AG1478, once every two days. The 24h after training, rats were anesthetized, the LVSP, LVEDP, ±dp/dt max were tested by carotid artery intubation which in order to evaluate cardiac function. The protein expression of NRG1, PI3K, Akt, eNOS, PKG, PLN, SERCA2a in myocardium were measured by Westernblotting, themRNA expression of serca2a were tested by RT-qPCR. \u0000Results Compared with S, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a decreased, serca2a mRNA expression decreased, LVSP and ±dp/dt max significantly decreased, LVEDP significantly increased; Compared with MI, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a increased, serca2a mRNA expression increased, LVSP and ±dp/dt max significantly increased, LVEDP decreased, and the effect of exercise were weaken by inhibitor AG1478. Correlation analysis showed that the myocardial pPLN and SERCA2a protein expression both were positively correlated with LVSP, ±dp/dtmax, and negatively correlated with LVEDP. \u0000Conclusions Intermittent exercise can increased myocardial NRG1 protein expression and activates NRG1-SERCA2a signaling pathway, improve myocardial infarction cardiac function.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82271129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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