Objective Vitamin D deficiency is widespread in postmenopausal women. It is verified that Vitamin D3 supplementation intake can improve the Vitamin D3 level of those Vitamin D deficiency patients. In addition to the exogenous intake, whether aerobic exercise plus sunshine could affect vitamin D level in postmenopausal women gained our attention. Methods 16 postmenopausal women in Shanghai attended this test. They voluntarily participated in a one year aerobics plan, practicing Chinese traditional dance outdoor under sunshine for one hour from 9:30-10:30 am each day. Before and after one year practice, serum 25(OH)D, 25(OH)D3 and estradiol E2, follicle stimulating hormone(FSH), luteinizing hormone(LH), parathyroid hormone(PTH) of all participants were analyzed. Results Before aerobics practice, serum 25(OH)D and 25(OH)D3 levels were 16.30±4.12(ng/ml) and 15.60±3.79(ng/ml). After one year practice, the data were significantly increased 19.50% (P=0.002) and 18.78% (P=0.002), separately. Before aerobics practice, the state of 25(OH)D level of 13 women was inadequacy (≤20.0ng/ml), 3 women was in lack status (20-30ng/ml). After one year practice, 9 women was inadequacy, 7 women in lack. The value of the chi square test was 4.747(P=0.029). After one year practice, serum PTH significantly increased, while FSH significantly decreased. E2 and LH had no significant variance before and after one year of aerobics practice. Conclusions One year aerobics practice under sunshine could increase serum 25(OH)D level, and affected estrogen levels variably in postmenopausal women.
{"title":"OR-013 One Year Outdoor and Daytime Aerobic Dance Practice Increased Serum 25(OH)D3 and PTH, but Decreased FSH Level of Postmenopausal Women","authors":"Qun Zuo, Yanyan Zhang","doi":"10.14428/EBR.V1I2.9133","DOIUrl":"https://doi.org/10.14428/EBR.V1I2.9133","url":null,"abstract":"Objective Vitamin D deficiency is widespread in postmenopausal women. It is verified that Vitamin D3 supplementation intake can improve the Vitamin D3 level of those Vitamin D deficiency patients. In addition to the exogenous intake, whether aerobic exercise plus sunshine could affect vitamin D level in postmenopausal women gained our attention. \u0000Methods 16 postmenopausal women in Shanghai attended this test. They voluntarily participated in a one year aerobics plan, practicing Chinese traditional dance outdoor under sunshine for one hour from 9:30-10:30 am each day. Before and after one year practice, serum 25(OH)D, 25(OH)D3 and estradiol E2, follicle stimulating hormone(FSH), luteinizing hormone(LH), parathyroid hormone(PTH) of all participants were analyzed. \u0000Results Before aerobics practice, serum 25(OH)D and 25(OH)D3 levels were 16.30±4.12(ng/ml) and 15.60±3.79(ng/ml). After one year practice, the data were significantly increased 19.50% (P=0.002) and 18.78% (P=0.002), separately. Before aerobics practice, the state of 25(OH)D level of 13 women was inadequacy (≤20.0ng/ml), 3 women was in lack status (20-30ng/ml). After one year practice, 9 women was inadequacy, 7 women in lack. The value of the chi square test was 4.747(P=0.029). After one year practice, serum PTH significantly increased, while FSH significantly decreased. E2 and LH had no significant variance before and after one year of aerobics practice. \u0000Conclusions One year aerobics practice under sunshine could increase serum 25(OH)D level, and affected estrogen levels variably in postmenopausal women. ","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86707948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective This study aims to explore the relationship between sedentary behavior and the diabetes mellitus prevalence of middle-aged and elderly people. Methods we conduct a questionnaire survey and physical examination for a total of 3,000 middle-aged and elderly people (≥45 years old) ,analysing by he software of SPSS21.0 and Stata12.0. Results The logistic regression analysis shows that the risk of diabetes is 1.617 (95% CI, 0.762-1.789, P <0.05) at 2-4h, 4-6h, 6-8h, ≥8h, = 0.003), 1.235 (95% CI, 0.818-1.865, P = 0.034), 3.420 (95% CI, 2.241-5.218, P = 0.000), 5.014 (95% CI, 3.049-8.247, P = 0.000). With each additional one-hour sedentariness the risk of diabetes increases by 23% (OR1.23, 95% CI 1.18-1.29, p <0.0001). Conclusions The sedentary behavior is an independent risk factor for diabetes. The prevalence of diabetes is gradually increasing with the increase of sedentary time, which indicates the longer sedentary time, the higher prevalence of diabetes.
目的探讨久坐行为与中老年人糖尿病患病率的关系。方法对3000名年龄≥45岁的中老年人进行问卷调查和体格检查，采用SPSS21.0和Stata12.0软件进行分析。结果logistic回归分析显示，2-4小时、4-6小时、6-8小时、≥8小时发生糖尿病的风险分别为1.617 (95% CI, 0.762 ~ 1.789, P <0.05)、1.235 (95% CI, 0.818 ~ 1.865, P = 0.034)、3.420 (95% CI, 2.241 ~ 5.218, P = 0.000)、5.014 (95% CI, 3.049 ~ 8.247, P = 0.000)。久坐时间每增加1小时，患糖尿病的风险增加23% (OR1.23, 95% CI 1.18-1.29, p <0.0001)。结论久坐行为是糖尿病的独立危险因素。糖尿病患病率随着久坐时间的增加而逐渐增加，这表明久坐时间越长，糖尿病患病率越高。
{"title":"PO-035 Association between sedentary behavior and diabetes mellitus in the prevalence of middle-aged and elderly people","authors":"Xinzheng Wang, Hongbin Luo","doi":"10.14428/EBR.V1I3.10003","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.10003","url":null,"abstract":"Objective This study aims to explore the relationship between sedentary behavior and the diabetes mellitus prevalence of middle-aged and elderly people. \u0000Methods we conduct a questionnaire survey and physical examination for a total of 3,000 middle-aged and elderly people (≥45 years old) ,analysing by he software of SPSS21.0 and Stata12.0. \u0000Results The logistic regression analysis shows that the risk of diabetes is 1.617 (95% CI, 0.762-1.789, P <0.05) at 2-4h, 4-6h, 6-8h, ≥8h, = 0.003), 1.235 (95% CI, 0.818-1.865, P = 0.034), 3.420 (95% CI, 2.241-5.218, P = 0.000), 5.014 (95% CI, 3.049-8.247, P = 0.000). With each additional one-hour sedentariness the risk of diabetes increases by 23% (OR1.23, 95% CI 1.18-1.29, p <0.0001). \u0000Conclusions The sedentary behavior is an independent risk factor for diabetes. The prevalence of diabetes is gradually increasing with the increase of sedentary time, which indicates the longer sedentary time, the higher prevalence of diabetes.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85321235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To investigate the effects of 4 weeks aerobic exercise mediates apelin on the p-AMPK(Thr172)/AMPK ratio in skeletal muscle of mice. Methods The C57BL/6J wild type mice(n=40) were randomly divided into four groups: control group (WC), exercise group (WE), apelin injection control group (AC) and apelin injection exercise group (AE), with 10 mice in each group. Apelin injection group mice were intraperitoneally injected with apelin (0.1 μmol/kg/day) for 4 weeks. At the same time, the exercise groups mice underwent 60min/day treadmill running with a slope of 5°at the speed of 15m/min for 2 weeks, and the speed was adjusted to 20m/min in the later 2 weeks. 48 h after the final exercise session quadriceps muscles were harvest. The protein expression of apelin, APJ, AMPKα and p-AMPKα (Thr172) in skeletal muscle was determined by Western Blot. Results (1) Compared with WC group, the protein expression of apelin , APJ and p-AMPKα (Thr172)/AMPKα ratio in AC group skeletal muscle of mice were increased; (2) Compared with WE group , the p-AMPKα (Thr172) / AMPKα ratio in AE group skeletal muscle of mice were increased. Conclusions Apelin supplementation for 4 weeks can up-regulate AMPK protein activity in skeletal muscle both in sedentary group and exercise group.
{"title":"PO-152 The effects of 4 weeks training mediates apelin on the p-AMPK(Thr172)/AMPK ratio in skeletal muscle of mice","authors":"Tieying Li, Ying Zhang","doi":"10.14428/EBR.V1I4.11763","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.11763","url":null,"abstract":"Objective To investigate the effects of 4 weeks aerobic exercise mediates apelin on the p-AMPK(Thr172)/AMPK ratio in skeletal muscle of mice. \u0000Methods The C57BL/6J wild type mice(n=40) were randomly divided into four groups: control group (WC), exercise group (WE), apelin injection control group (AC) and apelin injection exercise group (AE), with 10 mice in each group. Apelin injection group mice were intraperitoneally injected with apelin (0.1 μmol/kg/day) for 4 weeks. At the same time, the exercise groups mice underwent 60min/day treadmill running with a slope of 5°at the speed of 15m/min for 2 weeks, and the speed was adjusted to 20m/min in the later 2 weeks. 48 h after the final exercise session quadriceps muscles were harvest. The protein expression of apelin, APJ, AMPKα and p-AMPKα (Thr172) in skeletal muscle was determined by Western Blot. \u0000Results (1) Compared with WC group, the protein expression of apelin , APJ and p-AMPKα (Thr172)/AMPKα ratio in AC group skeletal muscle of mice were increased; \u0000(2) Compared with WE group , the p-AMPKα (Thr172) / AMPKα ratio in AE group skeletal muscle of mice were increased. \u0000Conclusions Apelin supplementation for 4 weeks can up-regulate AMPK protein activity in skeletal muscle both in sedentary group and exercise group.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"324 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86483099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Iirisin is a protein encoded by the target gene FNDC5 of peroxisome proliferator-activated receptor gamma coactivator in skeletal muscle. The secretory protein produced by shearing modification can promote the transformation of subcutaneous white fat into brown fat. As a kind of exercise-mediated muscle factor and potential fat factor, Irisin is closely related to obesity, insulin resistance, glucose and lipid metabolism, metabolic syndrome and so on. As an important preventive and therapeutic means of obesity, exercise plays a role in affecting irisin? Through the analysis of the effect of irisin on exercise intervention in obesity, this paper aims to lay a theoretical foundation for irisin to become a new way of thinking and a new target of treatment of obesity. Methods A computer-based search of the literature on "Research Progress in the Effects of Irisin on Exercise-induced Obesity" was conducted in the Sportdiscussussussand CNKI databases from 2012 to 2017. The key words were "obesity; exercise; Irisin; brown fat". Inclusion criteria were original research, Meta analysis and systematic review. Exclusion criteria: repetitive studies. A total of 86 articles were included in the review. Results (1) exercise can significantly improve obesity, insulin resistance, metabolic syndrome and other diseases;(2) Irisin can induce white fat Browning, increase body heat production, reduce body weight, and promote the expression of UCP1 by p38MAPK/ERK signaling pathway. Exogenous Irisin can significantly reduce obesity in mice induced by high-fat diet and improve insulin resistance.(3) whether it is one-time exercise or long-term exercise, endurance exercise or resistance exercise, moderate and low-intensity exercise or high-intensity exercise will increase the expression of irisin in skeletal muscle, blood or fat.However, the influence of different exercise intensity and different exercise modes on the expression of irisin is not regular, and the influence and mechanism of different exercise modes and exercise intensity on the expression of irisin between different species and different tissues have not been reported. Conclusions Exercise can significantly improve the occurrence and development of obesity, and the effect may be achieved by promoting the secretion and expression of irisin in skeletal muscle.
{"title":"PO-282 Effects Of Irises On Exercise To Improve Obesity","authors":"Wei-jie Zhu","doi":"10.14428/EBR.V1I5.12913","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.12913","url":null,"abstract":"Objective Iirisin is a protein encoded by the target gene FNDC5 of peroxisome proliferator-activated receptor gamma coactivator in skeletal muscle. The secretory protein produced by shearing modification can promote the transformation of subcutaneous white fat into brown fat. As a kind of exercise-mediated muscle factor and potential fat factor, Irisin is closely related to obesity, insulin resistance, glucose and lipid metabolism, metabolic syndrome and so on. As an important preventive and therapeutic means of obesity, exercise plays a role in affecting irisin? Through the analysis of the effect of irisin on exercise intervention in obesity, this paper aims to lay a theoretical foundation for irisin to become a new way of thinking and a new target of treatment of obesity. \u0000Methods A computer-based search of the literature on \"Research Progress in the Effects of Irisin on Exercise-induced Obesity\" was conducted in the Sportdiscussussussand CNKI databases from 2012 to 2017. The key words were \"obesity; exercise; Irisin; brown fat\". Inclusion criteria were original research, Meta analysis and systematic review. Exclusion criteria: repetitive studies. A total of 86 articles were included in the review. \u0000Results (1) exercise can significantly improve obesity, insulin resistance, metabolic syndrome and other diseases;(2) Irisin can induce white fat Browning, increase body heat production, reduce body weight, and promote the expression of UCP1 by p38MAPK/ERK signaling pathway. Exogenous Irisin can significantly reduce obesity in mice induced by high-fat diet and improve insulin resistance.(3) whether it is one-time exercise or long-term exercise, endurance exercise or resistance exercise, moderate and low-intensity exercise or high-intensity exercise will increase the expression of irisin in skeletal muscle, blood or fat.However, the influence of different exercise intensity and different exercise modes on the expression of irisin is not regular, and the influence and mechanism of different exercise modes and exercise intensity on the expression of irisin between different species and different tissues have not been reported. \u0000Conclusions Exercise can significantly improve the occurrence and development of obesity, and the effect may be achieved by promoting the secretion and expression of irisin in skeletal muscle.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87338647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective The irisin, recently identified novel molecule, exercise induced myokinehas，has been shown to be secreted from fibronectin type III domain containing 5 (FNDC5) of skeletal muscle by an unknown protease. It can drive brown-fat-like development of white fat, increase thermogenesis and lose weight. Apart from this, its expression and role in various other conditions such as inflammation, hippocampal neurogenesis, aging and other metabolic conditions have been reported. Moreover, it has benn reported that irisin play an important role in the regulation of various cardiovascular disease, such as endothelial disfunction, hypertension and atherosclerosis. The present review discussed the research progress of irisin in the field of sports, and the protective effects for cardiovascular disease. However, due to conflicting results, several issues have been raised regarding its expression, cleavage, circulating levels, detection, the form of exercise, etc. And it also discussed the current challenges and future perspectives. Methods Complete literature survey was performed using PubMed and WOS database search to gather available information regarding FNDC5/irisin. Results The present review discussed on the discovery of irisin, its possible role in the cardiovascular protection and its research progress in sport field. It provide a research direction and new perspective of the possible target for the prevention and treatment of related disease. Conclusions Irisin has a promising effect in predicting and diagnosing cardiovascular diseases, and the exercise level could be a influence factor. More research will be needed in the future.
{"title":"PO-141 The cardiovascular protection of irisin and its research progress in sport field","authors":"Shen Wang, Min Hu","doi":"10.14428/EBR.V1I4.10443","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.10443","url":null,"abstract":"Objective The irisin, recently identified novel molecule, exercise induced myokinehas，has been shown to be secreted from fibronectin type III domain containing 5 (FNDC5) of skeletal muscle by an unknown protease. It can drive brown-fat-like development of white fat, increase thermogenesis and lose weight. Apart from this, its expression and role in various other conditions such as inflammation, hippocampal neurogenesis, aging and other metabolic conditions have been reported. Moreover, it has benn reported that irisin play an important role in the regulation of various cardiovascular disease, such as endothelial disfunction, hypertension and atherosclerosis. The present review discussed the research progress of irisin in the field of sports, and the protective effects for cardiovascular disease. However, due to conflicting results, several issues have been raised regarding its expression, cleavage, circulating levels, detection, the form of exercise, etc. And it also discussed the current challenges and future perspectives. \u0000Methods Complete literature survey was performed using PubMed and WOS database search to gather available information regarding FNDC5/irisin. \u0000Results The present review discussed on the discovery of irisin, its possible role in the cardiovascular protection and its research progress in sport field. It provide a research direction and new perspective of the possible target for the prevention and treatment of related disease. \u0000Conclusions Irisin has a promising effect in predicting and diagnosing cardiovascular diseases, and the exercise level could be a influence factor. More research will be needed in the future. ","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"2017 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87457236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Exercise therapy has been widely used for the clinical treatment of Parkinson’s Disease (PD). However, the rehabilitation mechanisms involved remain clear. mGluRs play an important role in the progression of PD. In this study, immunohistochemistry and western blot analyses were used to detect the expression of presynaptic corticostriatal mGluR2/3 and postsynaptic mGluR5, and we attempted to investigate the molecular basis of the reconstruction of functional connectivity in the corticostriatal pathways from a mGluR perspective. Methods Male SD rats (230–250 g) were used as subjects in this experiment. After 1 week of habituation, the rats were randomly assigned to three groups: control group (Control, n = 24), PD group (PD, n = 24) and PD with exercise group (PD + Ex, n = 24). The experimental models were prepared by unilateral injection of 6-hydroxydopamine (6-OHDA) (2 μg/L, 8 μg) into the medial forebrain bundle, and the control group was administered an equivalent dose of saline solution. The models were evaluated with the apomorphine (APO)-induced rotation test. Two weeks postoperatively, exercise intervention was applied to the PD + Ex group for 4 weeks. Immunohistochemistry and western blot analysis were used to evaluate the expression of presynaptic corticostriatal mGluR2/3 and postsynaptic mGluR1/5. Results Results showed that the PD + Ex group had a significantly higher level of mGluR2/3 expression (P < 0.01) and significantly lower level of mGluR1/5 expression (P < 0.05) compared with the PD group. Conclusions Exercise intervention significantly increased the expression of mGluR2/3 and simultaneously reduced the expression of mGluR5, indicating that mGluRs can improve the behavioral function of PD rat models through exercise, and that the reconstruction of the functional connectivity of corticostriatal pathways plays an important role in nervous system regulation.
{"title":"PO-143 Effects of treadmill exercise on the expression of corticostriatal mGluRs in hemiparkinsonian rats","authors":"Kaixuan Shi, Xiaoli Liu, D. Qiao","doi":"10.14428/EBR.V1I4.10463","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.10463","url":null,"abstract":"Objective Exercise therapy has been widely used for the clinical treatment of Parkinson’s Disease (PD). However, the rehabilitation mechanisms involved remain clear. mGluRs play an important role in the progression of PD. In this study, immunohistochemistry and western blot analyses were used to detect the expression of presynaptic corticostriatal mGluR2/3 and postsynaptic mGluR5, and we attempted to investigate the molecular basis of the reconstruction of functional connectivity in the corticostriatal pathways from a mGluR perspective. \u0000Methods Male SD rats (230–250 g) were used as subjects in this experiment. After 1 week of habituation, the rats were randomly assigned to three groups: control group (Control, n = 24), PD group (PD, n = 24) and PD with exercise group (PD + Ex, n = 24). The experimental models were prepared by unilateral injection of 6-hydroxydopamine (6-OHDA) (2 μg/L, 8 μg) into the medial forebrain bundle, and the control group was administered an equivalent dose of saline solution. The models were evaluated with the apomorphine (APO)-induced rotation test. Two weeks postoperatively, exercise intervention was applied to the PD + Ex group for 4 weeks. Immunohistochemistry and western blot analysis were used to evaluate the expression of presynaptic corticostriatal mGluR2/3 and postsynaptic mGluR1/5. \u0000Results Results showed that the PD + Ex group had a significantly higher level of mGluR2/3 expression (P < 0.01) and significantly lower level of mGluR1/5 expression (P < 0.05) compared with the PD group. \u0000Conclusions Exercise intervention significantly increased the expression of mGluR2/3 and simultaneously reduced the expression of mGluR5, indicating that mGluRs can improve the behavioral function of PD rat models through exercise, and that the reconstruction of the functional connectivity of corticostriatal pathways plays an important role in nervous system regulation.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82260207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Intermittent exercise can improve cardiac function in rats with myocardial infarction. The Neuregulin-1(NRG1)/SERCA2a palys a critical role in maintain cardiac function. We want to investigate the effect of Neuregulin-1 (NRG1) on NRG1-SERCA2a signaling pathway activated by intermittent exercise and on improves cardiac function in rats with MI. Methods 32 male sprague-dawley rats were randomly divided into four groups (n=8): Sham-operated group (S), sedentary MI group (MI), MI with interval training group (ME), ME with inhibitor AG1478 group (MA). ME and MA model after the MI model was established by ligation of the left anterior descending coronary artery, and began training 1 week after MI surgery. The S model only by threading without ligation. Rats in ME and MA model taken one week adaptive training, then began 8-week interval training. MA model were injected with inhibitor AG1478, once every two days. The 24h after training, rats were anesthetized, the LVSP, LVEDP, ±dp/dt max were tested by carotid artery intubation which in order to evaluate cardiac function. The protein expression of NRG1, PI3K, Akt, eNOS, PKG, PLN, SERCA2a in myocardium were measured by Westernblotting, themRNA expression of serca2a were tested by RT-qPCR. Results Compared with S, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a decreased, serca2a mRNA expression decreased, LVSP and ±dp/dt max significantly decreased, LVEDP significantly increased; Compared with MI, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a increased, serca2a mRNA expression increased, LVSP and ±dp/dt max significantly increased, LVEDP decreased, and the effect of exercise were weaken by inhibitor AG1478. Correlation analysis showed that the myocardial pPLN and SERCA2a protein expression both were positively correlated with LVSP, ±dp/dtmax, and negatively correlated with LVEDP. Conclusions Intermittent exercise can increased myocardial NRG1 protein expression and activates NRG1-SERCA2a signaling pathway, improve myocardial infarction cardiac function.
{"title":"PO-144 Intermittent Exercise Activates NRG1-SERCA2a Pathway to Improve Cardiac Function in Myocardial Infarction Rats","authors":"Wenyan Bo, Dagang Li, Zhenjun Tian","doi":"10.14428/EBR.V1I4.10473","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.10473","url":null,"abstract":"Objective Intermittent exercise can improve cardiac function in rats with myocardial infarction. The Neuregulin-1(NRG1)/SERCA2a palys a critical role in maintain cardiac function. We want to investigate the effect of Neuregulin-1 (NRG1) on NRG1-SERCA2a signaling pathway activated by intermittent exercise and on improves cardiac function in rats with MI. \u0000Methods 32 male sprague-dawley rats were randomly divided into four groups (n=8): Sham-operated group (S), sedentary MI group (MI), MI with interval training group (ME), ME with inhibitor AG1478 group (MA). ME and MA model after the MI model was established by ligation of the left anterior descending coronary artery, and began training 1 week after MI surgery. The S model only by threading without ligation. Rats in ME and MA model taken one week adaptive training, then began 8-week interval training. MA model were injected with inhibitor AG1478, once every two days. The 24h after training, rats were anesthetized, the LVSP, LVEDP, ±dp/dt max were tested by carotid artery intubation which in order to evaluate cardiac function. The protein expression of NRG1, PI3K, Akt, eNOS, PKG, PLN, SERCA2a in myocardium were measured by Westernblotting, themRNA expression of serca2a were tested by RT-qPCR. \u0000Results Compared with S, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a decreased, serca2a mRNA expression decreased, LVSP and ±dp/dt max significantly decreased, LVEDP significantly increased; Compared with MI, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a increased, serca2a mRNA expression increased, LVSP and ±dp/dt max significantly increased, LVEDP decreased, and the effect of exercise were weaken by inhibitor AG1478. Correlation analysis showed that the myocardial pPLN and SERCA2a protein expression both were positively correlated with LVSP, ±dp/dtmax, and negatively correlated with LVEDP. \u0000Conclusions Intermittent exercise can increased myocardial NRG1 protein expression and activates NRG1-SERCA2a signaling pathway, improve myocardial infarction cardiac function.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82271129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Low-load blood flow restriction training (It's also called KAATSU) refers to the stimulation of skeletal muscle growth and muscle strength with low-load training intensity when the blood flow of the proximal venous vessels of the limbs is restricted for a short time. Thereby improving the muscle function of the skeletal muscle. KAATSU uses a low training load (20% IRM), which can play a medium to high load training effect. It is suitable for older people with weaker constitution, less skeletal muscle strength, and different degrees of aging muscle loss (sarcopenia). Therefore, there have been more and more studies on KAATSU intervention in chronic diseases in the elderly in recent years. This study systematically reviews the recent progress in the application of KAATSU in chronic diseases in the elderly, and provides a theoretical basis for the widespread application of KAATSU in the intervention of chronic diseases in the elderly. Methods Enter keywords in the websites of CNKI, CQVIP, Elsevier Science Direct, and Journals @Ovid Full Text, Pub Met, etc: “KAATSU, chronic disease”/“blood flow restriction, older”, a total of more than 3000 articles were searched, Screening of 98, At last, more than 40 literatures meeting the standards were analyzed and summarized. Results 1) Applied to skeletal muscle atrophy: KAATSU can significantly increase the cross-sectional area of skeletal muscle muscles, strengthen muscle strength, improve muscle contraction function, effectively prevent and interfere with sarcopenia, Especially for stroke patients with rehabilitation and long-term bed caused by muscular atrophy of disuse has a good clinical effect.2) Application to osteoporosis: KAATSU can improve bone mineral density, stimulate bone cell activity and improve bone health, but it is not yet certain whether to improve Senile osteoporosis. Future research should be combined with nutritional supplements to comprehensively evaluate its therapeutic effect. 3) Applied to cardiovascular and cerebrovascular diseases: Long-term KAATSU can effectively improve blood lipid metabolism, optimize cardiovascular function parameters, It doesn't activate the thrombin system, and has no significant negative impact on cardiovascular function in the elderly, the application effect is good in cardiovascular and cerebrovascular diseases such as atherosclerosis and hypertension.4) Application to metabolic syndrome: KAATSU can promote fat breakdown, reduce BMI,body fat percentage, insulin resistance index, glycosylated hemoglobin index, and improve diabetes and senile obesity in the elderly. 5) Others: There are a few studies showing that KAATSU can improve the daily activities of patients with Alzheimer's disease, promote the remodeling of bone tissue in elderly patients with osteonecrosis, and intervene with the muscle atrophy of patients with certain chronic heart diseases. Conclusions KAATSU is an effective exercise method for the elderly and has a good applica
{"title":"PO-287 Application of low-load blood flow restriction training in elderly chronic diseases","authors":"Jinna Cheng, Zhiguang Zhao","doi":"10.14428/EBR.V1I5.13123","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.13123","url":null,"abstract":"Objective Low-load blood flow restriction training (It's also called KAATSU) refers to the stimulation of skeletal muscle growth and muscle strength with low-load training intensity when the blood flow of the proximal venous vessels of the limbs is restricted for a short time. Thereby improving the muscle function of the skeletal muscle. KAATSU uses a low training load (20% IRM), which can play a medium to high load training effect. It is suitable for older people with weaker constitution, less skeletal muscle strength, and different degrees of aging muscle loss (sarcopenia). Therefore, there have been more and more studies on KAATSU intervention in chronic diseases in the elderly in recent years. This study systematically reviews the recent progress in the application of KAATSU in chronic diseases in the elderly, and provides a theoretical basis for the widespread application of KAATSU in the intervention of chronic diseases in the elderly. \u0000Methods Enter keywords in the websites of CNKI, CQVIP, Elsevier Science Direct, and Journals @Ovid Full Text, Pub Met, etc: “KAATSU, chronic disease”/“blood flow restriction, older”, a total of more than 3000 articles were searched, Screening of 98, At last, more than 40 literatures meeting the standards were analyzed and summarized. \u0000Results 1) Applied to skeletal muscle atrophy: KAATSU can significantly increase the cross-sectional area of skeletal muscle muscles, strengthen muscle strength, improve muscle contraction function, effectively prevent and interfere with sarcopenia, Especially for stroke patients with rehabilitation and long-term bed caused by muscular atrophy of disuse has a good clinical effect.2) Application to osteoporosis: KAATSU can improve bone mineral density, stimulate bone cell activity and improve bone health, but it is not yet certain whether to improve Senile osteoporosis. Future research should be combined with nutritional supplements to comprehensively evaluate its therapeutic effect. 3) Applied to cardiovascular and cerebrovascular diseases: Long-term KAATSU can effectively improve blood lipid metabolism, optimize cardiovascular function parameters, It doesn't activate the thrombin system, and has no significant negative impact on cardiovascular function in the elderly, the application effect is good in cardiovascular and cerebrovascular diseases such as atherosclerosis and hypertension.4) Application to metabolic syndrome: KAATSU can promote fat breakdown, reduce BMI,body fat percentage, insulin resistance index, glycosylated hemoglobin index, and improve diabetes and senile obesity in the elderly. 5) Others: There are a few studies showing that KAATSU can improve the daily activities of patients with Alzheimer's disease, promote the remodeling of bone tissue in elderly patients with osteonecrosis, and intervene with the muscle atrophy of patients with certain chronic heart diseases. \u0000Conclusions KAATSU is an effective exercise method for the elderly and has a good applica","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89285896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenqing Shen, Yi Zhang, Qiang Zhang, Zhengtang Qi, Yi Sun, Shuzhe Ding
Objective Type 2 diabetes mellitus is a common chronic diseases prevailing in the world and the amount of diabetic and pre-diabetic patients is increasing gradually. Exercise combined with hypoglycemic drug is the first recommended therapy to treat type 2 diabetes. Metformin was found from galegine in 1957 and has been used now as the first cheap and effective hypoglycemic guanidines. Our study aims to explore the effects of different ways of acute exercise intervention combined with high dose of metformin on glucose homeostasis and its relative molecular mechanisms in type 2 diabetic mice. Methods Adopt 4-week high fat diet (HFD, 45% fat content) and one-time STZ (Streptozocin, 100mg/kg) intraperitoneal injection to build type 2 diabetic mice. There are 84 mice in total, 24 mice were divided into three groups: normal control (NC) group, normal acute resistance training (NCR) group and normal acute endurance training (NCE) group, N=8 each group, they were fed normal chow. The rest 60 mice were fed HFD as T2D modeling group. 48 mice were developing type 2 diabetes and they were divided into 6 groups: diabetic control (DC) group, diabetic acute resistance training (DCR) group, diabetic acute endurance training (DCE) group, high dose of metformin control (HMC) group, high dose of metformin combined with acute resistance training (HMR) group and high dose of metformin combined with acute endurance training (HME) group, N=8 each group. Acute resistance training is climbing 1 meter ladder from down to up, 5 times a group, 3 groups in total, monitoring the glucose change with extracting mouse tail vein blood during each group, using ACCU-CHEK monitor. Acute endurance training is running at the speed of 18 m/min on the platform for 50 minutes and blood glucose change was monitored every 10 minutes by extracting mouse tail vein blood. HMC, HMR and HME group mice were intraperitoneally injected high dose of metformin (200mg/kg) one hour before the acute exercise intervention. Comparatively, NC, NCR, NCE, DC, DCR, DCE group mice were intraperitoneally injected 0.9% saline one hour before the acute exercise intervention. ELISA, RT-PCR and Western Blot were used to evaluate relative serum indicators, mRNA and protein expression of regulating blood glucose homeostasis. Results 1) 4-week high fat diet and one time 100mg/kg Streptozocin intraperitoneal injection induces mice to develop type 2 diabetes. The fasting blood glucose, IPGTT, ITT, glucose AUC and insulin AUC of T2D group mice are significantly higher than NC group. 2) Compared with DCR group, the blood glucose value and fluctuation of HMR group mice are both significantly decreased, but the blood glucose value of DCR and HMR group mice are significantly higher than NCR group. In the same way, the blood glucose value and fluctuation of HME group mice is lower than DCE group and the whole blood glucose level of both group are higher than NCE group. Acute resistance training and acute endurance training
{"title":"PO-062 Acute exercise intervention combined with metformin’s influences on glucose homeostasis in T2D mice","authors":"Wenqing Shen, Yi Zhang, Qiang Zhang, Zhengtang Qi, Yi Sun, Shuzhe Ding","doi":"10.14428/EBR.V1I3.10823","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.10823","url":null,"abstract":"Objective Type 2 diabetes mellitus is a common chronic diseases prevailing in the world and the amount of diabetic and pre-diabetic patients is increasing gradually. Exercise combined with hypoglycemic drug is the first recommended therapy to treat type 2 diabetes. Metformin was found from galegine in 1957 and has been used now as the first cheap and effective hypoglycemic guanidines. Our study aims to explore the effects of different ways of acute exercise intervention combined with high dose of metformin on glucose homeostasis and its relative molecular mechanisms in type 2 diabetic mice. \u0000Methods Adopt 4-week high fat diet (HFD, 45% fat content) and one-time STZ (Streptozocin, 100mg/kg) intraperitoneal injection to build type 2 diabetic mice. There are 84 mice in total, 24 mice were divided into three groups: normal control (NC) group, normal acute resistance training (NCR) group and normal acute endurance training (NCE) group, N=8 each group, they were fed normal chow. The rest 60 mice were fed HFD as T2D modeling group. 48 mice were developing type 2 diabetes and they were divided into 6 groups: diabetic control (DC) group, diabetic acute resistance training (DCR) group, diabetic acute endurance training (DCE) group, high dose of metformin control (HMC) group, high dose of metformin combined with acute resistance training (HMR) group and high dose of metformin combined with acute endurance training (HME) group, N=8 each group. Acute resistance training is climbing 1 meter ladder from down to up, 5 times a group, 3 groups in total, monitoring the glucose change with extracting mouse tail vein blood during each group, using ACCU-CHEK monitor. Acute endurance training is running at the speed of 18 m/min on the platform for 50 minutes and blood glucose change was monitored every 10 minutes by extracting mouse tail vein blood. HMC, HMR and HME group mice were intraperitoneally injected high dose of metformin (200mg/kg) one hour before the acute exercise intervention. Comparatively, NC, NCR, NCE, DC, DCR, DCE group mice were intraperitoneally injected 0.9% saline one hour before the acute exercise intervention. ELISA, RT-PCR and Western Blot were used to evaluate relative serum indicators, mRNA and protein expression of regulating blood glucose homeostasis. \u0000Results 1) 4-week high fat diet and one time 100mg/kg Streptozocin intraperitoneal injection induces mice to develop type 2 diabetes. The fasting blood glucose, IPGTT, ITT, glucose AUC and insulin AUC of T2D group mice are significantly higher than NC group. 2) Compared with DCR group, the blood glucose value and fluctuation of HMR group mice are both significantly decreased, but the blood glucose value of DCR and HMR group mice are significantly higher than NCR group. In the same way, the blood glucose value and fluctuation of HME group mice is lower than DCE group and the whole blood glucose level of both group are higher than NCE group. Acute resistance training and acute endurance training","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89489278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective The purpose was to further improve the understanding of exercise to improve dyslipidemia and to formulate exercise prescription more scientifically. It provides a reference for the further study of exercise mimics in the treatment of dyslipidemia and helps to reduce the high incidence of dyslipidemia. Methods Methods of documentation and comparative analysis are applied. Results It was found that the effects of exercise on dyslipidemia were beneficial or had no obvious effect on some indexes so far, and had no adverse effect. The biological mechanism of exercise regulating dyslipidemia and the effects of different exercise forms (acute exercise, long-term exercise training, different intensity exercise) on improving dyslipidemia were summarized. It is pointed out that exercise plays an important role in regulating the enzymes and proteins associated with dyslipidemia. Research from several aspects :exercise to block cholesterol biosynthesis, exercise inhibits cholesterol absorption, exercise affects cholesterol conversion, exercise promotes cholesterol conversion to bile acid, and exercise promotes cholesterol metabolism, exercise regulating triglycerides, etc. Conclusions The regulation of dyslipidemia is a complex process, involving multiple pathways, multiple gene regulation, and different hypolipidemic pathways.A large number of experimental studies have demonstrated the effect of exercise on the improvement of dyslipidemia, but there are few studies on the biological mechanism of exercise, which need to be further studied.In addition, when chemically synthesized anti-hyperlipidemia drugs have many safety problems, we should increase the in-depth study of sports drugs, especially some natural products, that can simulate exercise effectiveness. In order to better control the high incidence of dyslipidemia, it is necessary to improve the development of exercise mimic drugs in improving dyslipidemia.
{"title":"PO-130 Biological Mechanism of Exercise in Improving Dyslipidemia","authors":"Mi Tang, Q. Su","doi":"10.14428/EBR.V1I4.10323","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.10323","url":null,"abstract":"Objective The purpose was to further improve the understanding of exercise to improve dyslipidemia and to formulate exercise prescription more scientifically. It provides a reference for the further study of exercise mimics in the treatment of dyslipidemia and helps to reduce the high incidence of dyslipidemia. \u0000Methods Methods of documentation and comparative analysis are applied. \u0000Results It was found that the effects of exercise on dyslipidemia were beneficial or had no obvious effect on some indexes so far, and had no adverse effect. The biological mechanism of exercise regulating dyslipidemia and the effects of different exercise forms (acute exercise, long-term exercise training, different intensity exercise) on improving dyslipidemia were summarized. It is pointed out that exercise plays an important role in regulating the enzymes and proteins associated with dyslipidemia. Research from several aspects :exercise to block cholesterol biosynthesis, exercise inhibits cholesterol absorption, exercise affects cholesterol conversion, exercise promotes cholesterol conversion to bile acid, and exercise promotes cholesterol metabolism, exercise regulating triglycerides, etc. \u0000Conclusions The regulation of dyslipidemia is a complex process, involving multiple pathways, multiple gene regulation, and different hypolipidemic pathways.A large number of experimental studies have demonstrated the effect of exercise on the improvement of dyslipidemia, but there are few studies on the biological mechanism of exercise, which need to be further studied.In addition, when chemically synthesized anti-hyperlipidemia drugs have many safety problems, we should increase the in-depth study of sports drugs, especially some natural products, that can simulate exercise effectiveness. In order to better control the high incidence of dyslipidemia, it is necessary to improve the development of exercise mimic drugs in improving dyslipidemia.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89818174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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