Objective This article reviews the role of miRNAs in promoting insulin sensitivity, controlling insulin synthesis, and regulating insulin resistance in hypoxia exercise, and discusses the relationship between miRNAs and glucose metabolism, and the mechanism of hypoxia-induced regulation of miRNAs in glucose metabolism. It provides a theoretical basis for better research on the prevention and treatment of disorders of glucose metabolism. Methods This paper uses the literature data method, collects a large number of documents and cites more than 90 articles for comprehensive statistical analysis to write this article, which provides researchers with relevant research directions. Results Studies have shown that miRNAs such as miR-138, miR-26b, miR-27a, miR-122, miR-802 and miR-143 have regulatory effects on obesity; some miRNAs such as miR-128, miR-7, miR-25, miR -92a, miR-375 and miR-15 family (miR-15a and miR-15b) and other miRNAs play an important role in regulating glycolipid metabolism, thereby maintaining a stable state of glycolipid metabolism. Expressions such as miR-802 and miR-143 are up-regulated in the liver of obese patients, resulting in impaired glucose tolerance. Conclusions By regulating the expression of target genes and maintaining the homeostasis of glucose metabolism, miRNAs can effectively improve or prevent obesity and disorders of glucose metabolism such as type 2 diabetes. Current studies have shown that miRNAs affect glucose metabolism from insulin sensitivity, insulin synthesis and insulin resistance. At the same time, studies have shown that exercise intervention can effectively improve glucose metabolism. However, the research on the metabolism of miRNAs in glucose metabolism during hypoxia is still insufficient. It is for further study. Studying the mechanism of the effects of miRNAs on glucose metabolism in hypoxic exercise can not only provide a theoretical basis for scientific hypoglycemic and weight control, but also can be used as an intervention for the prevention and control of diseases related to glucose metabolism disorders. In the future, drugs can regulate the expression of miRNAs, thereby providing a new therapeutic approach for the treatment of diseases caused by abnormal glucose and lipid metabolism.
{"title":"PO-029 Advances in research on hypoxia-mediated miRNAs affecting glucose metabolism","authors":"Han Jiang","doi":"10.14428/EBR.V1I3.9933","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.9933","url":null,"abstract":"Objective This article reviews the role of miRNAs in promoting insulin sensitivity, controlling insulin synthesis, and regulating insulin resistance in hypoxia exercise, and discusses the relationship between miRNAs and glucose metabolism, and the mechanism of hypoxia-induced regulation of miRNAs in glucose metabolism. It provides a theoretical basis for better research on the prevention and treatment of disorders of glucose metabolism. \u0000Methods This paper uses the literature data method, collects a large number of documents and cites more than 90 articles for comprehensive statistical analysis to write this article, which provides researchers with relevant research directions. \u0000Results Studies have shown that miRNAs such as miR-138, miR-26b, miR-27a, miR-122, miR-802 and miR-143 have regulatory effects on obesity; some miRNAs such as miR-128, miR-7, miR-25, miR -92a, miR-375 and miR-15 family (miR-15a and miR-15b) and other miRNAs play an important role in regulating glycolipid metabolism, thereby maintaining a stable state of glycolipid metabolism. Expressions such as miR-802 and miR-143 are up-regulated in the liver of obese patients, resulting in impaired glucose tolerance. \u0000Conclusions By regulating the expression of target genes and maintaining the homeostasis of glucose metabolism, miRNAs can effectively improve or prevent obesity and disorders of glucose metabolism such as type 2 diabetes. Current studies have shown that miRNAs affect glucose metabolism from insulin sensitivity, insulin synthesis and insulin resistance. At the same time, studies have shown that exercise intervention can effectively improve glucose metabolism. However, the research on the metabolism of miRNAs in glucose metabolism during hypoxia is still insufficient. It is for further study. Studying the mechanism of the effects of miRNAs on glucose metabolism in hypoxic exercise can not only provide a theoretical basis for scientific hypoglycemic and weight control, but also can be used as an intervention for the prevention and control of diseases related to glucose metabolism disorders. In the future, drugs can regulate the expression of miRNAs, thereby providing a new therapeutic approach for the treatment of diseases caused by abnormal glucose and lipid metabolism.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"417 1-2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77837693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chonglin Zhang, Hui Wang, Xiaomin Ding, Shaosheng Liu, Z. Xia
Objective Both aerobic exercise and alimentary control relieve oxidative stress (OS). However, there may be different modes. The objective of this study is to identify the effects of OS in metabolic syndrome (MS) rats and explore the mechanisms involved in aerobic exercise and alimentary control. Methods Seventy rats were used. Eight rats were randomly chosen for the control group, while the remaining rats were subjected to MS using a high-fat diet for 18 wk. The MS model rats were subsequently divided into the MHC (model control with high-fat diet), MRC (model control with routine diet), MHE (model training with high-fat diet) and MRE (model training routine diet) groups for 12 wk. The chemotactic factor macrophage chemoattractant protein-1 (MCP-1), the adherence factor plasminogen activator inhibitor I (PAI-1), the oxidative stressor oxidized low density lipoprotein (ox-LDL), and the antioxidative factor endothelial nitric oxide synthase (eNOS) were tested in the serum; moreover, the expressions of MCP-1, PAI-1, and eNOS and the regulatory factor of OS Peroxisome proliferator-activated receptor-alpha (PPARα) were detected in the myocardium. Results OS related markers in serum had changes following the interventions of aerobic exercise and/or diet control. Compared with C, the ox-LDL, MCP-1 and PAI-1 in the MS rats exhibited significant decreases (P＜0.05/0.01), and the eNOS had a significant increase (P＜0.05). Compared with MHC, the diet intervention alone significantly increased eNOS; when interventions of aerobic exercise but not diet control (MHE) were applied, they significantly decreased ox-LDL, MCP-1 and PAI-1 (P＜0.01); the applied interventions of both aerobic exercise and diet control (MRE) decreased ox-LDL, MCP-1 and PAI-1 and significantly decreased eNOS (P＜0.01). The MRE compared to MHE exhibited significant changes in MCP-1 and eNOS (P＜0.01). The mRNA levels of the processing markers of OS in the myocardium. Compared with C, the MS rats had significant increases in the chemotactic factor MPC-1 (P＜0.05) and the adhesion factor PAI-1 (P＜0.01), which indicated that MS rats exhibited enhanced OS. Moreover, the MS rats had an increased antioxidant marker of eNOS, which was not significant, and the regulatory factor of PPARα decreased (P＜0.01). Compared to MHC, the MRC rats exhibited decreased MPC-1 (P＞0.05) and PAI-1 (P＜0.05) and increased eNOS (P＜0.01) and PPARα (P＞0.05); moreover, the MHE and MRE rats decreased more in MPC-1 and PAI-1 (P＜0.01) with increased eNOS and PPARα (P＜0.01). Compared to MHE, the MRE rats had a further decrease in MPC-1 (P＜0.01) and PAI-1 (P＞0.05) with increased eNOS (P＜0.05) and PPARα (P＞0.05). Conclusions OS increased in MS rats. Moreover, aerobic exercise and alimentary control could decrease OS to reduce the damage in MS rats. The OS regulatory factor PPARα, which could mediate the expression of OS-related genes, such as MCP-1, PAI-1, and eNOS in cardiovascular tissues, was only enhanced by aerobic exercis
{"title":"PO-250 Exercise and dietary intervention reduce myocardial oxidative stress in male rats with metabolic syndrome","authors":"Chonglin Zhang, Hui Wang, Xiaomin Ding, Shaosheng Liu, Z. Xia","doi":"10.14428/EBR.V1I5.10983","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.10983","url":null,"abstract":"Objective Both aerobic exercise and alimentary control relieve oxidative stress (OS). However, there may be different modes. The objective of this study is to identify the effects of OS in metabolic syndrome (MS) rats and explore the mechanisms involved in aerobic exercise and alimentary control. \u0000Methods Seventy rats were used. Eight rats were randomly chosen for the control group, while the remaining rats were subjected to MS using a high-fat diet for 18 wk. The MS model rats were subsequently divided into the MHC (model control with high-fat diet), MRC (model control with routine diet), MHE (model training with high-fat diet) and MRE (model training routine diet) groups for 12 wk. The chemotactic factor macrophage chemoattractant protein-1 (MCP-1), the adherence factor plasminogen activator inhibitor I (PAI-1), the oxidative stressor oxidized low density lipoprotein (ox-LDL), and the antioxidative factor endothelial nitric oxide synthase (eNOS) were tested in the serum; moreover, the expressions of MCP-1, PAI-1, and eNOS and the regulatory factor of OS Peroxisome proliferator-activated receptor-alpha (PPARα) were detected in the myocardium. \u0000Results OS related markers in serum had changes following the interventions of aerobic exercise and/or diet control. Compared with C, the ox-LDL, MCP-1 and PAI-1 in the MS rats exhibited significant decreases (P＜0.05/0.01), and the eNOS had a significant increase (P＜0.05). Compared with MHC, the diet intervention alone significantly increased eNOS; when interventions of aerobic exercise but not diet control (MHE) were applied, they significantly decreased ox-LDL, MCP-1 and PAI-1 (P＜0.01); the applied interventions of both aerobic exercise and diet control (MRE) decreased ox-LDL, MCP-1 and PAI-1 and significantly decreased eNOS (P＜0.01). The MRE compared to MHE exhibited significant changes in MCP-1 and eNOS (P＜0.01). The mRNA levels of the processing markers of OS in the myocardium. Compared with C, the MS rats had significant increases in the chemotactic factor MPC-1 (P＜0.05) and the adhesion factor PAI-1 (P＜0.01), which indicated that MS rats exhibited enhanced OS. Moreover, the MS rats had an increased antioxidant marker of eNOS, which was not significant, and the regulatory factor of PPARα decreased (P＜0.01). Compared to MHC, the MRC rats exhibited decreased MPC-1 (P＞0.05) and PAI-1 (P＜0.05) and increased eNOS (P＜0.01) and PPARα (P＞0.05); moreover, the MHE and MRE rats decreased more in MPC-1 and PAI-1 (P＜0.01) with increased eNOS and PPARα (P＜0.01). Compared to MHE, the MRE rats had a further decrease in MPC-1 (P＜0.01) and PAI-1 (P＞0.05) with increased eNOS (P＜0.05) and PPARα (P＞0.05). \u0000Conclusions OS increased in MS rats. Moreover, aerobic exercise and alimentary control could decrease OS to reduce the damage in MS rats. The OS regulatory factor PPARα, which could mediate the expression of OS-related genes, such as MCP-1, PAI-1, and eNOS in cardiovascular tissues, was only enhanced by aerobic exercis","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77219260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Gestational diabetes mellitus is the first time or any degree of glucose tolerance abnormalities discovered during the pregnancy. In the global the incidence of GDM can reach 15%. In China also reached 6.6%. Numerous studies have demonstrated that lifestyle intervention for the pregnant woman, especially to increase physical activity can significantly reduce the risk of GDM. Our research aims to search and analyze researches about the relationship of risk of gestational diabetes mellitus and physical activity intervention. Then we clear the effect of physical activity intervention and provide some advices for the future in this field. Methods The literature data were used though Pubmed, Web of Science, EBSCO, CNKI et al. database and we searched with the keywords of “physical activity”, “exercise”, “exercise intervention”, “gestational diabetes mellitus”, “glucose tolerance”, “postpartum diabetes prevention” in all these database. Finally we reviewed researches on physical activity interventions in GDM patients, discussed the relationship of before pregnancy, during pregnancy and postpartum physical activity intervention between the GDM risk. And we used the comparative analysis to get the corresponding conclusions. Results 1. Women of European and American countries during pregnancy who participated in physical activity and achieve the recommended amount was common, but only about 1/5 of Chinese women can achieve the physical activity amount which was recommended. 2. Join in regular physical activity before, during and after pregnancy, women could reduce the risk of GDM. 3. Aerobic training and/or resistance training could effectively improve glucose metabolism and insulin in patients with GDM and the recommended exercise prescription was moderate intensity, 3 to 5 days a week, last longer than 6 weeks. Conclusions The incidence of GDM is closely connected with pregnancy lifestyle changes. Women take part in regular physical activity before, during and after pregnancy could reduce the risk of GDM. But this kind of research is rare and behindhand in China. In the future, we should put forward the corresponding suggestions for our country to make pregnancy physical activity guidelines. In the other hand, researchers should verify the effect of health of pregnant women after the exercise intervention.
目的妊娠期糖尿病是妊娠期首次或任何程度的糖耐量异常。在全球范围内，GDM的发病率可达15%。中国也达到了6.6%。大量研究表明，对孕妇的生活方式进行干预，特别是增加身体活动，可以显著降低GDM的风险。本研究旨在搜集和分析有关妊娠期糖尿病发病风险与体育活动干预关系的研究。在此基础上，明确了体育活动干预的效果，并对今后体育活动干预的研究提出了建议。方法采用Pubmed、Web of Science、EBSCO、CNKI等数据库的文献资料，以“体力活动”、“运动”、“运动干预”、“妊娠期糖尿病”、“葡萄糖耐量”、“产后糖尿病预防”等关键词进行检索。最后回顾了体育活动干预对GDM患者的研究，探讨了孕前、孕期和产后体育活动干预与GDM风险的关系。并通过对比分析得出相应的结论。结果1。欧美国家的孕妇在孕期参加体育锻炼并达到推荐量的情况比较普遍，而我国只有约1/5的孕妇能达到推荐量。2. 在怀孕前、怀孕期间和怀孕后定期参加体育锻炼，可以降低女性患GDM的风险。3.有氧训练和/或阻力训练能有效改善GDM患者的糖代谢和胰岛素，推荐的运动处方为中等强度，每周3 - 5天，持续时间大于6周。结论妊娠期糖尿病的发生与妊娠生活方式的改变密切相关。妇女在怀孕前、怀孕期间和怀孕后定期进行体育锻炼可以降低患GDM的风险。但这种研究在中国很少见，也很落后。未来，我们应该为我国制定孕期体育活动指南提出相应的建议。另一方面，研究人员需要验证运动干预对孕妇健康的影响。
{"title":"PO-51 Status of Relationship of Risk of Gestational Diabetes Mellitus and Physical Activity intervention","authors":"Xian Guo, Yi Wang","doi":"10.14428/EBR.V1I3.10683","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.10683","url":null,"abstract":"Objective Gestational diabetes mellitus is the first time or any degree of glucose tolerance abnormalities discovered during the pregnancy. In the global the incidence of GDM can reach 15%. In China also reached 6.6%. Numerous studies have demonstrated that lifestyle intervention for the pregnant woman, especially to increase physical activity can significantly reduce the risk of GDM. Our research aims to search and analyze researches about the relationship of risk of gestational diabetes mellitus and physical activity intervention. Then we clear the effect of physical activity intervention and provide some advices for the future in this field. \u0000Methods The literature data were used though Pubmed, Web of Science, EBSCO, CNKI et al. database and we searched with the keywords of “physical activity”, “exercise”, “exercise intervention”, “gestational diabetes mellitus”, “glucose tolerance”, “postpartum diabetes prevention” in all these database. Finally we reviewed researches on physical activity interventions in GDM patients, discussed the relationship of before pregnancy, during pregnancy and postpartum physical activity intervention between the GDM risk. And we used the comparative analysis to get the corresponding conclusions. \u0000Results 1. Women of European and American countries during pregnancy who participated in physical activity and achieve the recommended amount was common, but only about 1/5 of Chinese women can achieve the physical activity amount which was recommended. 2. Join in regular physical activity before, during and after pregnancy, women could reduce the risk of GDM. 3. Aerobic training and/or resistance training could effectively improve glucose metabolism and insulin in patients with GDM and the recommended exercise prescription was moderate intensity, 3 to 5 days a week, last longer than 6 weeks. \u0000Conclusions The incidence of GDM is closely connected with pregnancy lifestyle changes. Women take part in regular physical activity before, during and after pregnancy could reduce the risk of GDM. But this kind of research is rare and behindhand in China. In the future, we should put forward the corresponding suggestions for our country to make pregnancy physical activity guidelines. In the other hand, researchers should verify the effect of health of pregnant women after the exercise intervention.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82522409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Diabetes is one kind of metabolic diseases characterized by hyperglycemia and metabolic syndrome due to insulin secretion defects or/and biological function damage. Evidences have suggested that sports plays essential role in the progression of diabetes and affects insulin resistance and monosaccharide transporter proteins activity. The purpose of this study was to investigate relationship between sports and diabetes by evaluation the insulin sensitivity and monosaccharide transporter proteins activity. Methods A db/db mice model of type-ІІ diabetes mellitus was used to analyze the efficacy of swimming in the processes of glucose and insulin. Body weight, glucose metabolism and insulin serum levels were studied in experimental mice. Expression levels of inflammatory factors of IL-1, IL-17, TGF-β and VEGF in the serum was analyzed in the experimental mice. Expression levels of sirtuin-1 and NF-κB signaling pathway was investigate in the lives. Histological analysis was studied the therapeutic effects of swimming on mice with type-ІІ diabetes. Results Our results indicated that swimming up-regulated metabolism of glucose and insulin. Sport of swimming enhanced the consumption of fat and fatty acid in the mice with diabetes. In addition, expression levels of inflammatory factors of IL-1, IL-17, TGF-β and VEGF in the serum were down-regulated by swimming in the mice with diabetes. Toll-like receptor 4 (TLR4) and sirtuin-1 expression levels were up-regulated in the liver in the experimental mice after receive swimming. Furthermore, we observed that swimming improved insulin sensitivity and monosaccharide transporter proteins (MTP) activity in liver in the experimental mice after receive swimming. Histological analysis showed that TTLR4 and sirtuin-1 accumulation were significantly decreased in the live in mice treated by swimming. NF-κB activity and expression levels of p65,IKK-β and IκBα were up-regulated in the mice receiving swimming. Conclusions In conclusion, these results indicate that swimming is beneficial for glucose and insulin metabolism for type-ІІ diabetes mice, which further improves metabolic syndrome by regulation insulin sensitivity and monosaccharide transporter proteins activity via NF-κB signaling pathway.
{"title":"PO-159 Swimming improves metabolic syndrome by regulation insulin sensitivity and monosaccharide transporter proteins activity via NF-κB signaling pathway","authors":"Sen Li, Jianya Huang","doi":"10.14428/ebr.v1i4.11863","DOIUrl":"https://doi.org/10.14428/ebr.v1i4.11863","url":null,"abstract":"Objective Diabetes is one kind of metabolic diseases characterized by hyperglycemia and metabolic syndrome due to insulin secretion defects or/and biological function damage. Evidences have suggested that sports plays essential role in the progression of diabetes and affects insulin resistance and monosaccharide transporter proteins activity. The purpose of this study was to investigate relationship between sports and diabetes by evaluation the insulin sensitivity and monosaccharide transporter proteins activity. \u0000Methods A db/db mice model of type-ІІ diabetes mellitus was used to analyze the efficacy of swimming in the processes of glucose and insulin. Body weight, glucose metabolism and insulin serum levels were studied in experimental mice. Expression levels of inflammatory factors of IL-1, IL-17, TGF-β and VEGF in the serum was analyzed in the experimental mice. Expression levels of sirtuin-1 and NF-κB signaling pathway was investigate in the lives. Histological analysis was studied the therapeutic effects of swimming on mice with type-ІІ diabetes. \u0000Results Our results indicated that swimming up-regulated metabolism of glucose and insulin. Sport of swimming enhanced the consumption of fat and fatty acid in the mice with diabetes. In addition, expression levels of inflammatory factors of IL-1, IL-17, TGF-β and VEGF in the serum were down-regulated by swimming in the mice with diabetes. Toll-like receptor 4 (TLR4) and sirtuin-1 expression levels were up-regulated in the liver in the experimental mice after receive swimming. Furthermore, we observed that swimming improved insulin sensitivity and monosaccharide transporter proteins (MTP) activity in liver in the experimental mice after receive swimming. Histological analysis showed that TTLR4 and sirtuin-1 accumulation were significantly decreased in the live in mice treated by swimming. NF-κB activity and expression levels of p65,IKK-β and IκBα were up-regulated in the mice receiving swimming. \u0000Conclusions In conclusion, these results indicate that swimming is beneficial for glucose and insulin metabolism for type-ІІ diabetes mice, which further improves metabolic syndrome by regulation insulin sensitivity and monosaccharide transporter proteins activity via NF-κB signaling pathway.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81349753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To explore the effects of different exercise patterns on gastrocnemius weight. Methods Forty-eight SD rats were randomly divided into 4 groups, which were quiet control group (Con), continuous exercise group (CE), high-intensity intermittent exercise group (HIIT) and ladder exercise group (LE). After 8 weeks of exercise training, each group was anesthetized and samples were collected for testing. The body weight and gastrocnemius weight of each group were recorded. The cross-sectional area (CSA) of gastrocnemius muscle was observed by HE staining. The levels of MSTN and insulin in serum were detected by ELISA. The expressions of MSTN, IGF1 and p70S6K in rat gastrocnemius muscle were detected by Western blot. Results Compared with group Con, the body weights of the three exercise groups were significantly lower, and the weight of the gastrocnemius muscles in group CE was significantly lower. Morphological observation of gastrocnemius showed that the cross-sectional area of the gastrocnemius muscle in group CE was significantly decreased, compared with group Con, and the cross-sectional area of the gastrocnemius muscle in group LE was significantly increased. There was no significant change in the cross-sectional area of the gastrocnemius muscle in group HIIT. The ELISA results showed that serum GDF8 levels were significantly decreased in the three exercise groups compared with group Con, while the insulin levels were not significantly changed. Compared with group Con, the expression of GDF8 protein in the gastrocnemius muscle of the group LE was significantly decreased, while the protein expression of IGF1 and P70S6K was significantly increased. The protein expression of the gastrocnemius P70S6K in group CE was also significantly increased. Conclusions Although the three exercise modes can significantly reduce the body weight of rats, only HIIT and LE improve the gastrocnemius muscle mass index. CE significantly reduced the body weight and cross-sectional area of the gastrocnemius muscle. Although the protein expression of P70S6K was increased, there was no significant effect on the protein expression of GDF8 and IGF1. Ladder movement may increase the gastrocnemius cross-sectional area by reducing MSTN and increasing protein expression of IGF1 and P70S6K.
{"title":"PO-155 Comparison of effects of different exercise modes on rat gastrocnemius muscle","authors":"Xiujuan Liu, Nianyun Zhang","doi":"10.14428/EBR.V1I4.11803","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.11803","url":null,"abstract":"Objective To explore the effects of different exercise patterns on gastrocnemius weight. \u0000Methods Forty-eight SD rats were randomly divided into 4 groups, which were quiet control group (Con), continuous exercise group (CE), high-intensity intermittent exercise group (HIIT) and ladder exercise group (LE). After 8 weeks of exercise training, each group was anesthetized and samples were collected for testing. The body weight and gastrocnemius weight of each group were recorded. The cross-sectional area (CSA) of gastrocnemius muscle was observed by HE staining. The levels of MSTN and insulin in serum were detected by ELISA. The expressions of MSTN, IGF1 and p70S6K in rat gastrocnemius muscle were detected by Western blot. \u0000Results Compared with group Con, the body weights of the three exercise groups were significantly lower, and the weight of the gastrocnemius muscles in group CE was significantly lower. Morphological observation of gastrocnemius showed that the cross-sectional area of the gastrocnemius muscle in group CE was significantly decreased, compared with group Con, and the cross-sectional area of the gastrocnemius muscle in group LE was significantly increased. There was no significant change in the cross-sectional area of the gastrocnemius muscle in group HIIT. The ELISA results showed that serum GDF8 levels were significantly decreased in the three exercise groups compared with group Con, while the insulin levels were not significantly changed. Compared with group Con, the expression of GDF8 protein in the gastrocnemius muscle of the group LE was significantly decreased, while the protein expression of IGF1 and P70S6K was significantly increased. The protein expression of the gastrocnemius P70S6K in group CE was also significantly increased. \u0000Conclusions Although the three exercise modes can significantly reduce the body weight of rats, only HIIT and LE improve the gastrocnemius muscle mass index. CE significantly reduced the body weight and cross-sectional area of the gastrocnemius muscle. Although the protein expression of P70S6K was increased, there was no significant effect on the protein expression of GDF8 and IGF1. Ladder movement may increase the gastrocnemius cross-sectional area by reducing MSTN and increasing protein expression of IGF1 and P70S6K.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82311058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective With the advent of the era of great health, people's health awareness is increasing.As an economical and environment-friendly way, exercise should be viewed as a “first line” strategy for prevention and treatment of arterial aging and a vital component of a contemporary public health approach for reducing the projected increase in population Cardiovascular diseases burden.The objective of this work was to study the effect of 8-week spin training and swim training on normal cyocardial cell cycle. Methods A total of 30 C57BL/6 mice were randomly allocated to 8-week spin training and swim training program (intervention group) or no supervised exercise (control group). Normal cyocardial cell cycle proteins were measured by Western Blotting. All the outcomes were measured after 8 weeks. Repeated measures were analyzed using mean ±SD and T tests were used to analyze the data. Results Compared with the control group, Western Blotting shows that CDK4 of spin training significantly increase (0.7698± 0.01130 to 1.111 ±0.02027, p<0.001) and CDK4 of swim training significantly increase (0.7698 ±0.01130 to 1.258± 0.01427, p<0.001) ,following 8 weeks of exercise, CyclinD1 of spin training increase (0.8558 ± 0.007912 to 1.499 ± 0.06303, p<0.001) and CyclinD1 of swim training significantly increase (0.8558 ± 0.007912 to 2.097 ± 0.3622, p<0.05) . Conclusions The 8-week spin training and swim training was found to be effective in increasing cyocardial cell cycles proteins in normal mice. There was significant improvement inCDK4 and CyclinD1 in intervention group compared to control group.
{"title":"PO-195 Effects of different exercises on cyocardial cell cycles","authors":"Jinfeng Xie","doi":"10.14428/EBR.V1I4.13043","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.13043","url":null,"abstract":"Objective With the advent of the era of great health, people's health awareness is increasing.As an economical and environment-friendly way, exercise should be viewed as a “first line” strategy for prevention and treatment of arterial aging and a vital component of a contemporary public health approach for reducing the projected increase in population Cardiovascular diseases burden.The objective of this work was to study the effect of 8-week spin training and swim training on normal cyocardial cell cycle. \u0000Methods A total of 30 C57BL/6 mice were randomly allocated to 8-week spin training and swim training program (intervention group) or no supervised exercise (control group). Normal cyocardial cell cycle proteins were measured by Western Blotting. All the outcomes were measured after 8 weeks. Repeated measures were analyzed using mean ±SD and T tests were used to analyze the data. \u0000Results Compared with the control group, Western Blotting shows that CDK4 of spin training significantly increase (0.7698± 0.01130 to 1.111 ±0.02027, p<0.001) and CDK4 of swim training significantly increase (0.7698 ±0.01130 to 1.258± 0.01427, p<0.001) ,following 8 weeks of exercise, CyclinD1 of spin training increase (0.8558 ± 0.007912 to 1.499 ± 0.06303, p<0.001) and CyclinD1 of swim training significantly increase (0.8558 ± 0.007912 to 2.097 ± 0.3622, p<0.05) . \u0000Conclusions The 8-week spin training and swim training was found to be effective in increasing cyocardial cell cycles proteins in normal mice. There was significant improvement inCDK4 and CyclinD1 in intervention group compared to control group.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87625420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Study of the effect of treadmill resistance training on the cross section and phosphorylation of ERK1/2 and p38MAPK of gastrocnemius of aging rats, and explore the role of resistance training in prevention and regulation of sarcopenia. Methods 11-month-old male Sprague-Dawley (SD) rats (n = 40, SPF grade, initial body weight 776.9 ± 50) were raised in various cages in a room with a room temperature of 23 ± 2°C, a humidity of 40–60%, and a natural light/dark cycle. After eight months, the treated rats were then divided into one sedentary group (control, n = 8/group) and four groups for resistance training (GRT 1-GRT 4, GRT for Group for Resistance Training, n = 8/group) lasting for 8 weeks. For resistance training, GRT1 to GRT4 carried no loading, 30% maximum loading, 50% maximum loading and 70% maximum loading, respectively. The protocol for treadmill running was as follows: 15m/min (speed), 35°C (slope), 15s each time, an interval for 30s between two runnings, four runnings as one training, an interval for 3mins between two training, three training as one cycle, two cycles one training day, and one-day break between two training days. The morphology and cross section of gastrocnemius and the phosphorylation of the ERK1/2、p38MAPK proteins in the rats were examined after 8 weeks’ training. Results Compared with the control, GRT1-GRT4 displayed relatively better aligned muscles fibers and noticeably larger cross-sectional area (P＜0.05). The content of p-ERK1/2 (phosphorylated ERK1/2) displayed a descending trend as the loading increased compared with the control (GRT1 VS control, P＞0.05; GRT2 VS control, P＜0.05; GRT3 VS control, P＞0.05; GRT4 VS control, P＞0.05). However, the pairwise comparison revealed that only the difference between GRT1 and GRT2 was significant. Similarly, the content of p-p38MAPK (phosphorylated p38MAPK) displayed a descending trend as the loading increased compared with the control (GRT1 VS control, P＞0.05; GRT2 VS control, P＞0.05; GRT3 VS control, P＜0.01; GRT4 VS control, P＜0.01). Besides, the pairwise comparison revealed that the difference between GRT1 and GRT3 was significant (P＜0.05) and that between GRT1 and GRT4 was even highly significant (P＜0.01). Conclusions Resistance training with middle-class intensity or above can prevent sarcopenia. ERK1/2 and p38MAPK were subject to high-level phosphorylation in aging skeletal muscles, which resulted from the stress-like status of the aging body. Treadmill training of with middle-class intensity or above can reduce ERK1/2 and p38MAPK phosphorylation in aging skeletal muscles of rats, which indicates that resistance training can effectively prevent sarcopenia by inhibiting ERK1/2 and p38MAPK phosphorylation.
{"title":"PO-179 Prevention of sarcopenia and regulation of the ERK1/2 and p38MAPK phosphorylation by resistance training","authors":"Hao Chen, Xiquan Weng, Wentao Lin, Sanhua Zhai","doi":"10.14428/EBR.V1I4.12703","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.12703","url":null,"abstract":"Objective Study of the effect of treadmill resistance training on the cross section and phosphorylation of ERK1/2 and p38MAPK of gastrocnemius of aging rats, and explore the role of resistance training in prevention and regulation of sarcopenia. \u0000Methods 11-month-old male Sprague-Dawley (SD) rats (n = 40, SPF grade, initial body weight 776.9 ± 50) were raised in various cages in a room with a room temperature of 23 ± 2°C, a humidity of 40–60%, and a natural light/dark cycle. After eight months, the treated rats were then divided into one sedentary group (control, n = 8/group) and four groups for resistance training (GRT 1-GRT 4, GRT for Group for Resistance Training, n = 8/group) lasting for 8 weeks. For resistance training, GRT1 to GRT4 carried no loading, 30% maximum loading, 50% maximum loading and 70% maximum loading, respectively. The protocol for treadmill running was as follows: 15m/min (speed), 35°C (slope), 15s each time, an interval for 30s between two runnings, four runnings as one training, an interval for 3mins between two training, three training as one cycle, two cycles one training day, and one-day break between two training days. The morphology and cross section of gastrocnemius and the phosphorylation of the ERK1/2、p38MAPK proteins in the rats were examined after 8 weeks’ training. \u0000Results Compared with the control, GRT1-GRT4 displayed relatively better aligned muscles fibers and noticeably larger cross-sectional area (P＜0.05). The content of p-ERK1/2 (phosphorylated ERK1/2) displayed a descending trend as the loading increased compared with the control (GRT1 VS control, P＞0.05; GRT2 VS control, P＜0.05; GRT3 VS control, P＞0.05; GRT4 VS control, P＞0.05). However, the pairwise comparison revealed that only the difference between GRT1 and GRT2 was significant. Similarly, the content of p-p38MAPK (phosphorylated p38MAPK) displayed a descending trend as the loading increased compared with the control (GRT1 VS control, P＞0.05; GRT2 VS control, P＞0.05; GRT3 VS control, P＜0.01; GRT4 VS control, P＜0.01). Besides, the pairwise comparison revealed that the difference between GRT1 and GRT3 was significant (P＜0.05) and that between GRT1 and GRT4 was even highly significant (P＜0.01). \u0000Conclusions Resistance training with middle-class intensity or above can prevent sarcopenia. ERK1/2 and p38MAPK were subject to high-level phosphorylation in aging skeletal muscles, which resulted from the stress-like status of the aging body. Treadmill training of with middle-class intensity or above can reduce ERK1/2 and p38MAPK phosphorylation in aging skeletal muscles of rats, which indicates that resistance training can effectively prevent sarcopenia by inhibiting ERK1/2 and p38MAPK phosphorylation.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87169993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Exercise stimulation to the body of the first stress: blood changes, regulated by the renin-angiotensin system. Long-term high-intensity exercise training will lead to changes in kidney structure and function, resulting in renal injury. This will not only affect training and competition, but also affect the health of athletes, thus it is becoming an influential factor in the occurrence and development of excessive fatigue. At present, the mechanism of renal injury and proteinuria caused by exercise is not very clear. Therefore, this study conducted an in-depth study on the upstream mechanism of renal blood flow changes from the molecular level and explored the effects of 6-week high-intensity exercise training on the renal local renin-angiotensin system in rats. Methods 30 SD male rats (8 weeks old) were randomly divided into control group (10) and exercise group (20). Then according to different time, the exercise group (20) was divided into two groups on average, which are 0h group and 24h group. The rats in the control group did not do any exercise, and the rats in the exercise group were trained to run on the platform with high intensity for 6 weeks. At the beginning of the sixth week, the urine of rats from different group were selected randomly, and the urine NGAL, urinary microalbumin (mAlb), urinary creatinine (UCr) and total urine protein (TP) were tested, to determine the rat model of exercise-induced renal injury with proteinuria. After training on the 6th weekend, the renal tissue renin activity and angiotensin Ⅱ (Ang Ⅱ) content in right kidney were tested. At the same time, the left kidney of the rat was taken to make HE staining sections. Results (1) The mAlb, Alb/Cr and TP of the exercise group were higher than those of the control group, while the UCr content was lower than that of the control group. There was a significant difference between mAlb and TP in the exercise group and the control group(p<0.05). UCr, mAlb/Cr were significantly different from the control group(p<0.01). The urine NGAL concentration in the exercise group was higher than that in the control group, and the difference was significant(p<0.05). (2) The glomerulus of the rats in the 0h group showed obvious congestion, swelling and erythrocyte exudation. The tissue morphology of rats in the 24h group recovered slightly, but it was still different from the control group. (3) The renin activity and Ang Ⅱ were the lowest in the 0h group, and the renin activity and Ang Ⅱ were highest in the 24h group. There is a very significant difference between the groups(p<0.01). Conclusions (1) The 6-week high-intensity training used in this study increased the levels of NGAL, TP, and mAlb in the urine of rats, and successfully established a rat model of exercise-induced renal injury with proteinuria. (2) Long-term high-intensity exercise training can cause obvious congestion, swelling, erythrocyte exudation in rat glomeruli, which can’t return to the quiet level 24
{"title":"PO-099 Effects of long-term high-intensity exercise training on renal local renin-angiotensin system in rats","authors":"Z. Dang, Jianmin Cao, Xian Guo, Y. Niu, Han Xiao","doi":"10.14428/EBR.V1I3.11733","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.11733","url":null,"abstract":"Objective Exercise stimulation to the body of the first stress: blood changes, regulated by the renin-angiotensin system. Long-term high-intensity exercise training will lead to changes in kidney structure and function, resulting in renal injury. This will not only affect training and competition, but also affect the health of athletes, thus it is becoming an influential factor in the occurrence and development of excessive fatigue. At present, the mechanism of renal injury and proteinuria caused by exercise is not very clear. Therefore, this study conducted an in-depth study on the upstream mechanism of renal blood flow changes from the molecular level and explored the effects of 6-week high-intensity exercise training on the renal local renin-angiotensin system in rats. \u0000Methods 30 SD male rats (8 weeks old) were randomly divided into control group (10) and exercise group (20). Then according to different time, the exercise group (20) was divided into two groups on average, which are 0h group and 24h group. The rats in the control group did not do any exercise, and the rats in the exercise group were trained to run on the platform with high intensity for 6 weeks. At the beginning of the sixth week, the urine of rats from different group were selected randomly, and the urine NGAL, urinary microalbumin (mAlb), urinary creatinine (UCr) and total urine protein (TP) were tested, to determine the rat model of exercise-induced renal injury with proteinuria. After training on the 6th weekend, the renal tissue renin activity and angiotensin Ⅱ (Ang Ⅱ) content in right kidney were tested. At the same time, the left kidney of the rat was taken to make HE staining sections. \u0000Results (1) The mAlb, Alb/Cr and TP of the exercise group were higher than those of the control group, while the UCr content was lower than that of the control group. There was a significant difference between mAlb and TP in the exercise group and the control group(p<0.05). UCr, mAlb/Cr were significantly different from the control group(p<0.01). The urine NGAL concentration in the exercise group was higher than that in the control group, and the difference was significant(p<0.05). (2) The glomerulus of the rats in the 0h group showed obvious congestion, swelling and erythrocyte exudation. The tissue morphology of rats in the 24h group recovered slightly, but it was still different from the control group. (3) The renin activity and Ang Ⅱ were the lowest in the 0h group, and the renin activity and Ang Ⅱ were highest in the 24h group. There is a very significant difference between the groups(p<0.01). \u0000Conclusions (1) The 6-week high-intensity training used in this study increased the levels of NGAL, TP, and mAlb in the urine of rats, and successfully established a rat model of exercise-induced renal injury with proteinuria. (2) Long-term high-intensity exercise training can cause obvious congestion, swelling, erythrocyte exudation in rat glomeruli, which can’t return to the quiet level 24","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88113226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Under the natural and comfortable condition of the private human body, explore which of the running and walking is more conducive to the physical health of the human body. Methods Among the sports crowd of the NanMingRiver Rubber Exercise Road in Guiyang City, select 20 people who have walking exercise habits and who can take the running test under the age of 60 as group A and select 20 people who have running exercise habits and who can take the walking test under the age of 60 as group B. Inclusion criteria for the exercise habits is “Exercise at least 3 times a week for more than 30 minutes and for more than one year.” (1) Experimental method: Two groups of subjects with different exercise modes adopted the exercise mode of the corresponding group. Under the condition that other environmental conditions were unchanged, the health benefits of different exercise methods in the same group were compared before and after the experiment. The time of experiment lasts three months. The intensity of walking exercise is the intensity that the athlete feels natural and comfortable. The intensity of running exercise is the intensity that the athlete feels natural and comfortable. Before and after the experiment，the test contents of the two group subjects included height, weight, BMI, grip strength, reaction time, closed-eyes standing on a leg, sitting body flexion, step test, lung capacity, lung capacity index of body mass. (2) Literature and Information method: Through the retrieval and review of relevant literatures on sports and physical health at home and abroad, this paper provides theoretical references and methodological basis for this research. (3) Data analysis method: Paired sample t-test was used to analyze the changes of constitution of the two groups experimental people before and after the exercise modes. Results After a 3-month walking in group A, the step test, lung capacity, and lung capacity index of body mass were higher than before, and the difference was statistically significant (P<0.01). The grip strength, reaction time, closed-eyes standing on a leg decreased compared with prior exercise. The difference was statistically significant (P<0.05); body mass, body mass index (BMI), and sitting body flexion were higher than before, but the difference was not statistically significant. After three months of walking exercise in group B, the step test, lung capacity, lung capacity index of body mass, and grip strength decreased compared with the previous exercise, and the difference was statistically significant (P<0.01); body mass , body mass index (BMI), reaction time, the closed-eye standing on one foot was lower than before, and the difference was statistically significant (P<0.05). The anterior flexion of the sitting body decreased, but the difference was not statistically significant. Conclusions In comfortable, natural conditions, running is more conducive to physical fitness than walking, and running is a more effect
{"title":"PO-117 To compare the impact of running and walking on physical health","authors":"Xianfeng Hua","doi":"10.14428/EBR.V1I4.9943","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.9943","url":null,"abstract":"Objective Under the natural and comfortable condition of the private human body, explore which of the running and walking is more conducive to the physical health of the human body. \u0000Methods Among the sports crowd of the NanMingRiver Rubber Exercise Road in Guiyang City, select 20 people who have walking exercise habits and who can take the running test under the age of 60 as group A and select 20 people who have running exercise habits and who can take the walking test under the age of 60 as group B. Inclusion criteria for the exercise habits is “Exercise at least 3 times a week for more than 30 minutes and for more than one year.” \u0000(1) Experimental method: Two groups of subjects with different exercise modes adopted the exercise mode of the corresponding group. Under the condition that other environmental conditions were unchanged, the health benefits of different exercise methods in the same group were compared before and after the experiment. The time of experiment lasts three months. The intensity of walking exercise is the intensity that the athlete feels natural and comfortable. The intensity of running exercise is the intensity that the athlete feels natural and comfortable. Before and after the experiment，the test contents of the two group subjects included height, weight, BMI, grip strength, reaction time, closed-eyes standing on a leg, sitting body flexion, step test, lung capacity, lung capacity index of body mass. \u0000(2) Literature and Information method: Through the retrieval and review of relevant literatures on sports and physical health at home and abroad, this paper provides theoretical references and methodological basis for this research. \u0000(3) Data analysis method: Paired sample t-test was used to analyze the changes of constitution of the two groups experimental people before and after the exercise modes. \u0000Results After a 3-month walking in group A, the step test, lung capacity, and lung capacity index of body mass were higher than before, and the difference was statistically significant (P<0.01). The grip strength, reaction time, closed-eyes standing on a leg decreased compared with prior exercise. The difference was statistically significant (P<0.05); body mass, body mass index (BMI), and sitting body flexion were higher than before, but the difference was not statistically significant. \u0000After three months of walking exercise in group B, the step test, lung capacity, lung capacity index of body mass, and grip strength decreased compared with the previous exercise, and the difference was statistically significant (P<0.01); body mass , body mass index (BMI), reaction time, the closed-eye standing on one foot was lower than before, and the difference was statistically significant (P<0.05). The anterior flexion of the sitting body decreased, but the difference was not statistically significant. \u0000Conclusions In comfortable, natural conditions, running is more conducive to physical fitness than walking, and running is a more effect","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86383608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mallikarjuna Korivi, Yubo Liu, Weibing Ye, Yong Zhang, Sathyavelu R. Kesireddy
Objective Alcohol consumption particularly at old age can cause severe liver damage through malfunctioning of vital organelles, including mitochondria. Exercise is known to improve the cellular functions against alcohol-induced adverse effects and oxidative stress. Nevertheless, whether exercise can promote mitochondrial function in old alcohol-fed rats remains unclear. In this study, we investigated the effect of exercise training on intra- and extra-mitochondrial enzyme activities in alcohol/ethanol treated rats. Methods Young (3-month, n=24) and old (18-month, n=24) Wistar albino rats were equally divided into control, exercise, ethanol and combination of exercise plus ethanol treated groups. Following treadmill exercise (23 m/min, 30 min/day 5-day/wk) and ethanol (2 g/kg b.w.) treatment for 2 months, cytosol and mitochondrial enzyme activities, triglycerides and phospholipids were estimated in the liver of young and old rats. Results We found ethanol intoxication significantly decreased (P<0.01) the hepatic intra- and extra-mitochondrial enzyme activities, including glucose-6-phosphate dehydrogenase (G6PD), succinate dehydrogenase (SDH), malate dehydrogenase (MDH) and glutamate dehydrogenase (GDH) in both young and old rats. However, exercise training considerably reversed the loss of these enzyme activities, and further maintained above control levels in respective age groups. Restoration of mitochondrial marker enzymes (SDH and GDH) with exercise against ethanol-loss was prominent in young compared to old rats, which indicates old rats are prone to alcohol-induced adverse effects. Alcohol-induced elevated LDH levels in both ages were slightly decreased by exercise plus ethanol treatment. We further noticed that amplified triglycerides and phospholipids were substantially decreased following treadmill exercise in both age groups. Decreased triglycerides level with exercise was prominent in young alcohol-fed rats than that of old. Conclusions Our results imply that 2-month treadmill exercise training effectively ameliorated the ruined cytosol and mitochondrial enzyme activities in young and old ethanol-fed rats. Improved mitochondrial enzymes and decreased triglycerides with exercise training may protect the alcohol-induced liver damage.
{"title":"PO-230 Treadmill Running Ameliorates Alcohol-Induced Malfunctioning of Intra- and Extra-Mitochondrial Enzymes in Liver of Aged Rats","authors":"Mallikarjuna Korivi, Yubo Liu, Weibing Ye, Yong Zhang, Sathyavelu R. Kesireddy","doi":"10.14428/EBR.V1I5.10593","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.10593","url":null,"abstract":"Objective Alcohol consumption particularly at old age can cause severe liver damage through malfunctioning of vital organelles, including mitochondria. Exercise is known to improve the cellular functions against alcohol-induced adverse effects and oxidative stress. Nevertheless, whether exercise can promote mitochondrial function in old alcohol-fed rats remains unclear. In this study, we investigated the effect of exercise training on intra- and extra-mitochondrial enzyme activities in alcohol/ethanol treated rats. \u0000Methods Young (3-month, n=24) and old (18-month, n=24) Wistar albino rats were equally divided into control, exercise, ethanol and combination of exercise plus ethanol treated groups. Following treadmill exercise (23 m/min, 30 min/day 5-day/wk) and ethanol (2 g/kg b.w.) treatment for 2 months, cytosol and mitochondrial enzyme activities, triglycerides and phospholipids were estimated in the liver of young and old rats. \u0000Results We found ethanol intoxication significantly decreased (P<0.01) the hepatic intra- and extra-mitochondrial enzyme activities, including glucose-6-phosphate dehydrogenase (G6PD), succinate dehydrogenase (SDH), malate dehydrogenase (MDH) and glutamate dehydrogenase (GDH) in both young and old rats. However, exercise training considerably reversed the loss of these enzyme activities, and further maintained above control levels in respective age groups. Restoration of mitochondrial marker enzymes (SDH and GDH) with exercise against ethanol-loss was prominent in young compared to old rats, which indicates old rats are prone to alcohol-induced adverse effects. Alcohol-induced elevated LDH levels in both ages were slightly decreased by exercise plus ethanol treatment. We further noticed that amplified triglycerides and phospholipids were substantially decreased following treadmill exercise in both age groups. Decreased triglycerides level with exercise was prominent in young alcohol-fed rats than that of old. \u0000Conclusions Our results imply that 2-month treadmill exercise training effectively ameliorated the ruined cytosol and mitochondrial enzyme activities in young and old ethanol-fed rats. Improved mitochondrial enzymes and decreased triglycerides with exercise training may protect the alcohol-induced liver damage. \u0000 ","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86187747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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