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Immunohistochemical markers in the determination of lesion viability in decomposed bodies: A mini literature review 免疫组化标记在确定腐尸病变存活率中的作用:小型文献综述。
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-11-05 DOI: 10.1016/j.forsciint.2024.112289
Cecilia Salzillo , Liliana Innamorato , Alessia Leggio , Andrea Marzullo
The ability to distinguish between viable and post-mortem lesions is crucial in forensic medicine, especially in cases of advanced decomposition. Glycophorin A (GPA) is one of the most studied immunohistochemical markers for assessing lesion viability, with persistence in putrefied tissues up to 6 months. However, its sensitivity decreases beyond 15 days. Other markers such as tryptase, IL-15, CD15, CD45 and matrix metalloproteinases have been tested in decomposition contexts, but the results are inconclusive. This mini review article explores the main studies on immunohistochemical markers, discussing their utility and limitations in forensic investigations of decomposed bodies.
在法医学中,尤其是在晚期腐烂的情况下,区分存活病变和死后病变的能力至关重要。Glycophorin A(GPA)是研究最多的评估病变存活能力的免疫组化标记物之一,在腐败组织中的存活时间长达 6 个月。不过,超过 15 天后,其敏感性就会下降。其他标记物,如胰蛋白酶、IL-15、CD15、CD45 和基质金属蛋白酶也在分解过程中进行了测试,但结果并不确定。这篇小型综述文章探讨了有关免疫组化标记物的主要研究,讨论了这些标记物在法医调查腐烂尸体时的作用和局限性。
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引用次数: 0
Felid scavenging in forensic taphonomic research: An experimental approach 法证岩石学研究中的猫科动物食腐:实验方法
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-11-04 DOI: 10.1016/j.forsciint.2024.112280
D. Errickson , L. Lawrence , L. Indra , T.J.U. Thompson
Animal scavenging on human remains presents a major challenge at a forensic scene. These lasting changes can influence the interpretation of a post-mortem interval, the overall state of the remains and any associated evidence, impacting the integrity of the scene. Therefore, identifying taphonomic related changes due to animal scavenging is important to understand the post-deposition sequence of events. However, knowing where to look is challenging and animal scavenging studies are difficult to create. Therefore, this study worked in collaboration with zoological institutes in the UK to capture the scavenging changes to horse bone, focussing on the locations of scavenging on bone and the characteristics left. This study focused on large felid (cheetah, lion, tiger, leopard) scavenging, which is less documented in comparison to canine scavenging. This research demonstrated the distribution patterns of tooth activity associated with large felid scavenging is consistent with those reported in the taphonomic literature on lions. Specifically, pits, punctures, scalloping and furrowing were found and characteristics were frequently noted at the borders and flat regions of bones. This study adds to the forensic discussion of scavenging. While focussing on large cats, the work demonstrates anatomical regions that may be affected by scavenging and the visual cues that may help identify animal interaction over human.
动物对遗骸的啃食是法医现场的一大挑战。这些持久的变化会影响对死后时间间隔的解释、遗骸的整体状态以及任何相关证据,从而影响现场的完整性。因此,确定动物食腐引起的与岩石学相关的变化对于了解沉积后的事件顺序非常重要。然而,知道在哪里寻找具有挑战性,而且动物食腐研究很难进行。因此,本研究与英国的动物研究所合作,捕捉马骨的食腐变化,重点研究食腐在马骨上的位置和留下的特征。这项研究的重点是大型猫科动物(猎豹、狮子、老虎、豹)的食腐,与犬科动物的食腐相比,大型猫科动物的食腐记录较少。这项研究表明,与大型猫科动物食腐相关的牙齿活动分布模式与有关狮子的古生物学文献报道一致。具体地说,在骨骼的边缘和平坦区域发现了凹坑、穿刺、扇形和沟纹等特征。这项研究为有关食腐的法医学讨论增添了新的内容。这项研究以大型猫科动物为重点,展示了可能受到食腐影响的解剖区域,以及有助于识别动物与人类互动的视觉线索。
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引用次数: 0
Accuracy and reproducibility of bullet comparison decisions by forensic examiners 法医检验人员做出子弹对比决定的准确性和可重复性。
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-11-04 DOI: 10.1016/j.forsciint.2024.112287
R. Austin Hicklin , Connie L. Parks , Kensley M. Dunagan , Brandi L. Emerick , Nicole Richetelli , William J. Chapman , Melissa Taylor , Robert M. Thompson
Few previous studies have assessed the accuracy and reproducibility of bullet comparison decisions by firearms examiners, and none have evaluated accuracy of examiners’ decisions when comparing damaged bullets, comparisons of questioned bullets, or the effects on decision rates of using jacketed hollow-point vs. full metal jacket bullets. In this study, 49 practicing forensic firearms examiners conducted 3156 comparisons of bullets, including bullets ranging in quality, bullets from different types of ammunition, and bullets fired from various makes/models of firearms. The study evaluated two scenarios commonly used in casework: questioned-questioned (QQ) comparisons of two bullets from unknown sources, and known-questioned (KQ) comparisons in which a bullet from an unknown source is compared to three known exemplars from a single firearm. Key findings: after controlling for other factors, QQ vs. KQ comparisons had relatively limited effects on decision rates; rates of inconclusive responses were inversely related to bullet quality; bullets fired from polygonally-rifled pistols resulted in more inconclusive or unsuitable responses than conventional rifling; on nonmated comparison sets, the rate of (true) exclusions was particularly high when comparing different caliber bullets, and was higher on comparisons of different makes/models of firearms vs. the same model of firearm; comparisons in which different types of ammunition were fired from the same firearm had a high rate of erroneous exclusions; decision rates differed notably by firearm model; decision rates varied notably among the participants. Because the measured rates vary dramatically due to these various factors, we recommend against using overall decision rates to summarize the results of this study.

Significance statement

Comparisons of bullets by forensic firearms examiners are a critical part of the criminal justice system, seeking to determine whether recovered bullets from crime scenes can be attributed to specific firearms. This paper reports the results of a research study designed to assess the accuracy and reliability of forensic bullet comparison decisions, which is important to assess scientific validity for admissibility in court. Few studies have been conducted assessing the accuracy of forensic bullet comparison decisions, and no previous studies have evaluated the accuracy and reproducibility of decisions made by practicing forensic firearms examiners when comparing bullets of varying quality or bullets of different types, or when making comparisons of questioned bullets.
之前很少有研究对枪支检验人员做出的子弹比对决定的准确性和可重复性进行评估,也没有研究对检验人员在比对受损子弹时做出决定的准确性、对有疑问的子弹进行比对或使用护套空尖弹与全金属护套弹对决定率的影响进行评估。在这项研究中,49 名执业法医枪支检验员对 3156 发子弹进行了比较,包括不同质量的子弹、不同类型弹药的子弹以及不同品牌/型号枪支发射的子弹。研究评估了案件工作中常用的两种情况:来源不明的两颗子弹的 "质疑-提问"(QQ)比较和 "已知-提问"(KQ)比较,其中 "质疑-提问 "比较是将来源不明的一颗子弹与来自同一枪支的三颗已知样弹进行比较。主要发现:在控制了其他因素后,QQ 与 KQ 对比对判定率的影响相对有限;不确定的回答率与子弹质量成反比;与传统膛线相比,多边形膛线手枪发射的子弹导致更多不确定或不合适的回答;在非配枪对比集上,比较不同口径子弹时的(真实)排除率特别高,比较不同品牌/型号枪支与相同型号枪支时的(真实)排除率更高;在配枪对比集上,比较不同品牌/型号枪支时的(真实)排除率特别高,比较不同品牌/型号枪支与相同型号枪支时的(真实)排除率更高。不同型号枪支与同一型号枪支的比较中,错误排除率较高;同一枪支发射不同类型弹药的比较中,错误排除率较高;不同型号枪支的判定率差异显著;不同参与者的判定率差异显著。由于上述各种因素导致测得的判定率差异巨大,我们建议不要使用总体判定率来概括本研究的结果。意义声明:法医枪支检验人员对子弹进行比较是刑事司法系统的重要组成部分,目的是确定从犯罪现场找到的子弹是否可归属于特定枪支。本文报告了一项研究的结果,该研究旨在评估法医子弹比对决定的准确性和可靠性,这对于评估法庭受理的科学有效性非常重要。很少有研究对法医子弹比对决定的准确性进行评估,以前也没有研究对执业法医枪支检验员在比对不同质量的子弹或不同类型的子弹时,或在比对可疑子弹时所作决定的准确性和可重复性进行评估。
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引用次数: 0
Analysis of the health conditions of migrants and asylum seekers in reception centers: From scene investigation to the evaluation of signs of torture 分析接待中心移民和寻求庇护者的健康状况:从现场调查到酷刑迹象评估。
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-11-04 DOI: 10.1016/j.forsciint.2024.112288
Massimiliano Esposito , Monica Salerno , Mario Giuseppe Chisari , Francesco Sessa , Venerando Rapisarda , Cristoforo Pomara
The phenomenon of migration is an international issue that mainly concerns Europe. In Italy, because of its close proximity to Africa, there are many migrant landings, especially on the islands of Lampedusa and Sicily. Migrants and asylum seekers suffer torture on their journey to their destination country, however, most of the time the signs are not always recognizable. In the present study, a scene investigation was carried out in a reception center for level II immigrants in Sicily, in order to evaluate the hygiene conditions of the migrants and asylum seekers inside this center. Subsequently 26 migrants and asylum seekers, all minors and males, were examined in a clinic of the Department of Forensic Medicine of the University of Catania, with the help of a native speaker interpreter and applying the Istanbul Protocol. The scene investigation demonstrated the poor clinical conditions of the reception center and the failure to comply with emergency regulations. The physical examination of the migrants and asylum seekers demonstrated that all the torture reported had taken place in Libya. However, only 4 migrants and asylum seekers showed signs of violence consistent with torture, compatible with the Istanbul Protocol. Nine migrants and asylum seekers (34.6 %) reported having pathologies and asked to undergo a medical examination in a hospital but were not listened to by the reception center staff. Two migrants and asylum seekers reported having worn the same clothes for several weeks to the reception center staff. In particular, all the migrants and asylum seekers reported having suffered torture using blunt instruments, especially beatings with truncheons. One migrant and asylum seeker reported being detained for days without food, instead 1 migrants and asylum seekers reported being kidnapped-blindfolded-stripped naked for days, and 1 migrants and asylum seekers reported having contracted infections, including scabies, with electrical and thermal trauma. Despite the international commitment to the phenomenon of migration, migrants and asylum seekers continue to receive abuse and torture. Furthermore, most migrants and asylum seekers studies primarily involve surveys, and few have applied the Istanbul Protocol to a sizable sample. An original aim of the study is the use of forensic tools to assess the sanitary and hygienic conditions of a migrant reception center, with the secondary aim of assessing alleged cases of torture.
移民现象是一个主要涉及欧洲的国际问题。在意大利,由于毗邻非洲,有许多移民登陆,特别是在兰佩杜萨岛和西西里岛。移民和寻求庇护者在前往目的地国的途中遭受酷刑,但大多数情况下,这些迹象并不总能被识别出来。本研究在西西里岛的一个二级移民接待中心进行了现场调查,以评估该中心内移民和寻求庇护者的卫生条件。随后,26 名移民和寻求庇护者(均为未成年人和男性)在卡塔尼亚大学法医系的一家诊所接受了检查,检查由一名母语为翻译的人员协助,并采用了《伊斯坦布尔议定书》。现场调查表明,接待中心的医疗条件很差,而且没有遵守紧急条例。对移民和寻求庇护者的身体检查表明,所有报告的酷刑都发生在利比亚。然而,只有 4 名移民和寻求庇护者出现了符合《伊斯坦布尔议定书》规定的酷刑暴力迹象。九名移民和寻求庇护者(占 34.6%)报告称患有疾病,并要求到医院进行体检,但接待中心的工作人员没有听取他们的意见。两名移民和寻求庇护者向接待中心工作人员报告说,他们几个星期都穿着同样的衣服。特别是,所有移民和寻求庇护者都报告说遭受了使用钝器的酷刑,尤其是用警棍殴打。一名移徙者和寻求庇护者报告说,他们被拘留数日,没有食物,一名移徙者和寻求庇护者报告说,他们被绑架--蒙上眼睛--赤身裸体数日,一名移徙者和寻求庇护者报告说,他们因电击和热创伤而感染疾病,包括疥疮。尽管国际社会对移徙现象做出了承诺,但移徙者和寻求庇护者继续受到虐待和酷刑。此外,大多数关于移民和寻求庇护者的研究主要涉及调查,很少有研究将《伊斯坦布尔议定书》应用于相当大的样本中。这项研究的最初目的是使用法医工具评估移民接待中心的卫生条件,其次是评估指控的酷刑案件。
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引用次数: 0
Determination of endogenous GHB in ante-mortem whole blood, urine, and oral fluid by LC–MS/MS: The effect of different additives and storage conditions on the stability of GHB in blood 利用LC-MS/MS测定死前全血、尿液和口服液中的内源性伽马--羟丁酸:不同添加剂和储存条件对血液中伽马--羟丁酸稳定性的影响
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-11-01 DOI: 10.1016/j.forsciint.2024.112286
Lambert K. Sørensen, Kathrine B. Faldborg, Charlotte U. Andersen, Jørgen B. Hasselstrøm
Two challenges in detecting γ-hydroxybutyric acid (GHB) intake are its endogenous presence and in vitro production after sampling. This study developed an LC–MS/MS method for selective GHB determination in human antemortem blood, urine, and oral fluid at endogenous concentrations. Furthermore, the stability of GHB in blood samples and its endogenous concentrations in samples taken under controlled circumstances were investigated. Samples were extracted in methanol/acetonitrile and processed by anion exchange solid-phase extraction. GHB was separated from structural isomers using a reversed–phase LC column with anion properties. The validated limit of quantification was 0.005 µg/mL in blood and 0.010 µg/mL in urine and oral fluid, at which the relative reproducibility standard deviation and bias were <15 %. The mean extraction recovery was ≥90 %. The average GHB concentration increased by 1.2 µg/mL in fluoride/citrate- preserved blood after 28 days of storage at 4°C; however, in fluoride/oxalate (FX)-preserved blood, the mean concentration increased by only 0.055 µg/mL. No change was observed at −20°C. In 105 randomly selected samples of FX-preserved blood collected for forensic antemortem toxicological analysis, all concentrations were <0.066 µg/mL, even after long-term storage at −20°C. In blood, urine, and oral fluid samples from a clinical study of GHB intake, endogenous baseline levels from 30 participants ranged from 0.0069–0.050, 0.024–0.38, and 0.034–0.93 µg/mL, respectively. These results demonstrate that the current cut-off level of 5 µg/mL for discriminating between endogenous and exogenous GHB in antemortem blood could be considerably lower for FX-preserved blood stored at −20°C.
检测γ-羟丁酸(GHB)摄入量的两个难题是其内源性存在和采样后的体外生成。本研究开发了一种 LC-MS/MS 方法,用于选择性地检测人体死前血液、尿液和口服液中内源性浓度的 GHB。此外,还研究了血液样本中 GHB 的稳定性以及在受控情况下采集样本中 GHB 的内源性浓度。样品在甲醇/乙腈中提取,并通过阴离子交换固相萃取法进行处理。使用具有阴离子特性的反相液相色谱柱从结构异构体中分离出 GHB。血液中的有效定量限为 0.005 µg/mL,尿液和口服液中的有效定量限为 0.010 µg/mL,相对重现性标准偏差和偏差为 15%。平均提取回收率≥90%。在 4°C 下保存 28 天后,氟化物/柠檬酸盐保存的血液中 GHB 的平均浓度增加了 1.2 µg/mL;但在氟化物/草酸盐(FX)保存的血液中,平均浓度仅增加了 0.055 µg/mL。在 -20°C 下则未观察到任何变化。在为法医尸检毒理学分析而随机抽取的 105 份 FX 保存血液样本中,即使在 -20°C 长期储存后,所有浓度仍为 0.066 µg/mL。在一项关于伽马--羟丁酸摄入量的临床研究中,30 名参与者的血液、尿液和口腔液样本中的内源性基线水平分别为 0.0069-0.050、0.024-0.38 和 0.034-0.93 微克/毫升。这些结果表明,目前用于鉴别死前血液中内源性和外源性 GHB 的 5 µg/mL 临界值,对于储存在 -20°C 的 FX 保存血液来说可能要低得多。
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引用次数: 0
Research progress and application of bacterial traceability technology 细菌溯源技术的研究进展与应用。
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-10-31 DOI: 10.1016/j.forsciint.2024.112275
Wei Wang , Bichun Zhao , Hanyu Zhang , Zhaowei Jie , Can Hu , Hongling Guo , Ping Wang , Yajun Li , Jun Zhu , Hongcheng Mei , Jian Ye
Bacterial traceability refers to the use of a range of techniques to trace the origins and transmission pathways of bacteria. It is crucial in controlling the spread of diseases, analyzing bioterrorism incidents, and advancing microbial forensics. In recent years, the frequency and scope of bacterial outbreaks have continued to escalate, exerting significant impacts on global biosecurity, public health, and other areas. Consequently, it is required to process traceability of bacteria timely and accurately around the globe. The rapid development of biological and physicochemical traceability techniques provides convenience for tracing bacteria. These techniques not only surpass traditional methods in terms of sensitivity, traceability and throughput, but also find more extensive applications in elucidating bacterial growth mechanisms, transmission routes, and geographical origins. This paper systematically reviews the latest research progress and applications of technologies of bacterial traceability, highlighting key advancements and projecting future trends, with the intent of providing a valuable reference for researchers, facilitating further studies and innovations in this field.
细菌溯源是指使用一系列技术来追踪细菌的来源和传播途径。它对于控制疾病传播、分析生物恐怖主义事件和推进微生物法医学至关重要。近年来,细菌爆发的频率和范围不断升级,对全球生物安全、公共卫生和其他领域造成了重大影响。因此,需要在全球范围内及时、准确地对细菌进行溯源处理。生物和理化溯源技术的快速发展为细菌溯源提供了便利。这些技术不仅在灵敏度、可追溯性和通量方面超越了传统方法,而且在阐明细菌的生长机制、传播途径和地理起源方面也有更广泛的应用。本文系统回顾了细菌溯源技术的最新研究进展和应用,重点介绍了主要进展并预测了未来趋势,旨在为研究人员提供有价值的参考,促进该领域的进一步研究和创新。
{"title":"Research progress and application of bacterial traceability technology","authors":"Wei Wang ,&nbsp;Bichun Zhao ,&nbsp;Hanyu Zhang ,&nbsp;Zhaowei Jie ,&nbsp;Can Hu ,&nbsp;Hongling Guo ,&nbsp;Ping Wang ,&nbsp;Yajun Li ,&nbsp;Jun Zhu ,&nbsp;Hongcheng Mei ,&nbsp;Jian Ye","doi":"10.1016/j.forsciint.2024.112275","DOIUrl":"10.1016/j.forsciint.2024.112275","url":null,"abstract":"<div><div>Bacterial traceability refers to the use of a range of techniques to trace the origins and transmission pathways of bacteria. It is crucial in controlling the spread of diseases, analyzing bioterrorism incidents, and advancing microbial forensics. In recent years, the frequency and scope of bacterial outbreaks have continued to escalate, exerting significant impacts on global biosecurity, public health, and other areas. Consequently, it is required to process traceability of bacteria timely and accurately around the globe. The rapid development of biological and physicochemical traceability techniques provides convenience for tracing bacteria. These techniques not only surpass traditional methods in terms of sensitivity, traceability and throughput, but also find more extensive applications in elucidating bacterial growth mechanisms, transmission routes, and geographical origins. This paper systematically reviews the latest research progress and applications of technologies of bacterial traceability, highlighting key advancements and projecting future trends, with the intent of providing a valuable reference for researchers, facilitating further studies and innovations in this field.</div></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"365 ","pages":"Article 112275"},"PeriodicalIF":2.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A first step towards a machine learning-based framework for bloodstain classification in forensic science 基于机器学习的法医学血迹分类框架的第一步
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-10-31 DOI: 10.1016/j.forsciint.2024.112278
Hyeonah Jung , Yeon-Soo Jo , Yoseop (Joseph) Ahn , Jaehoon (Paul) Jeong , Si-Keun Lim
Bloodstains found at a crime scene can help estimate the events that occurred during the crime. Reconstructing the crime scene by analyzing the bloodstain pattern contributes to understanding the bloody event. Therefore, it is essential to classify bloodstains through bloodstain pattern analysis (BPA) and accurately estimate the actions that took place at that time. In this study, we investigate the potential of using machine learning and deep learning to determine an action related to bloodstain data through the accessment of the corresponding bloodstain type by creating a prototype classification model. There are 14 types of bloodstain according to the classification system based on appearance. In this study, we test the classification potential of each bloodstain data for three bloodstain patterns such as Swing, Cessation, and Impact. Through experiments, it is shown that our prototype classification model for the selected bloodstains is developed and the accuracy of the resulting model is evaluated to be 80 %.
在犯罪现场发现的血迹有助于估计犯罪过程中发生的事件。通过分析血迹模式重建犯罪现场有助于了解血腥事件。因此,必须通过血迹模式分析(BPA)对血迹进行分类,并准确估计当时发生的行为。在本研究中,我们通过创建一个原型分类模型,研究了使用机器学习和深度学习通过评估相应的血迹类型来确定与血迹数据相关的行动的潜力。根据基于外观的分类系统,血迹共有 14 种类型。在本研究中,我们测试了每种血迹数据对 Swing、Cessation 和 Impact 等三种血迹模式的分类潜力。实验结果表明,我们为所选血迹建立了原型分类模型,模型的准确率达到 80%。
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引用次数: 0
Forensic investigation into head trauma in combat sports, blunt force homicides, and traditional martial arts 对格斗运动、钝器杀人和传统武术中头部创伤的法医调查
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-10-31 DOI: 10.1016/j.forsciint.2024.112269
Andreas Bourantanis , Konstantinos Katsos , Alexandros Samolis , Dimitrios Vlachodimitropoulos , George Troupis , Emmanouil I. Sakelliadis , Weijie W. Wang

Background

The analysis of circumstantial elements in post-mortem examinations is crucial for identifying perpetrators in unarmed close-range homicides. This study explores the forensic analysis of head trauma fatalities related to combat sports, blunt force trauma homicides, and traditional martial arts (TMA), aiming to bridge the gap between historical martial arts documentation and modern forensic pathology.

Materials and methods

Following PRISMA guidelines, we conducted a systematic literature review and interdisciplinary analysis combining traditional forensic methods with the study of TMA manuscripts and consultations with martial arts experts.

Results

The research uncovered a high incidence of head injuries in combat sports and identified distinct injury patterns in blunt-force trauma homicides. In martial arts, lethal techniques targeting the cranial region were pinpointed. An algorithm was developed to differentiate between injuries from various sources effectively.

Conclusion

This study offers a framework to distinguish injuries from combat sports, homicides, and martial arts, improving diagnostic accuracy in forensic pathology. It advocates for a detailed protocol that considers the technical aspects of TMA and their forensic implications, enhancing the evaluation of head trauma cases in forensic investigations.
背景分析尸体检验中的环境因素对于确定徒手近距离杀人案件中的犯罪者至关重要。本研究探讨了与格斗运动、钝器击伤凶杀案和传统武术(TMA)相关的头部创伤死亡案例的法医分析,旨在弥合历史武术文献与现代法医病理学之间的差距。材料与方法根据PRISMA指南,我们结合传统法医学方法、传统武术手稿研究和武术专家咨询,进行了系统的文献综述和跨学科分析。在武术中,针对颅骨区域的致命技术被精确定位。本研究提供了一个区分格斗运动、凶杀和武术伤害的框架,提高了法医病理学诊断的准确性。它提倡制定详细的规程,考虑 TMA 的技术方面及其对法医的影响,从而加强法医调查中对头部创伤病例的评估。
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引用次数: 0
Baclofen-related deaths in Australia 2000–2022 2000-2022 年澳大利亚与巴氯芬相关的死亡人数
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-10-30 DOI: 10.1016/j.forsciint.2024.112281
Emma Zahra , Shane Darke , Julia Lappin , Johan Duflou , Michael Farrell
In Australia, the therapeutic indication of baclofen (oral tablets) is for the treatment of muscle spasm. Deaths related to baclofen have been reported, as well as misuse, dependence and self-poisoning. This national retrospective study aimed to investigate the number, characteristics, circumstances, and toxicology of baclofen-related deaths in Australia, 2000–2022. We identified 102 baclofen-related deaths, with a mean age of 45.6 years and 51 % being male. Circumstances of death were: intentional toxicity (54.9 %), unintentional toxicity (30.4 %), unintentional toxicity/disease (9.8 %), and accidental injury (4.9 %). Multiple sclerosis or spinal injury was documented in 15.7 % of cases, substance use problems in 43.1 % and specifically alcohol use problems in 38.2 %. Mental health problems were documented in 73.5 %, a previous self-harm or suicide attempt in 30.4 %, and chronic pain in 37.3 %. The median baclofen blood concentration for all cases was 3.10 mg/L (25th 0.70, 75th 8.10, range 0.04–110.00), unintentional toxicity 1.95 mg/L (25th 0.70, 75th 4.35, range 0.04–24.0), intentional toxicity 6.00 mg/L (25th 1.10, 75th 13.0, range 0.05–110.0). Concomitant substance use was seen in 93.8 %, with antidepressants (69.8 %) and benzodiazepines (64.6 %) most frequently detected. In conclusion, the ‘typical’ case was middle-aged, most dying due to intentional toxicity, and likely to have a history of mental health and substance use problems. We suggest caution is needed in prescribing baclofen given its potential to be used in intentional and non-intentional overdose.
在澳大利亚,巴氯芬(口服片剂)的治疗适应症是治疗肌肉痉挛。与巴氯芬有关的死亡以及滥用、依赖和自我中毒事件时有报道。这项全国性的回顾性研究旨在调查2000-2022年间澳大利亚与巴氯芬相关的死亡病例的数量、特征、情况和毒理学。我们共发现 102 例巴氯芬相关死亡病例,平均年龄为 45.6 岁,51% 为男性。死亡原因包括:有意中毒(54.9%)、无意中毒(30.4%)、无意中毒/疾病(9.8%)和意外伤害(4.9%)。多发性硬化症或脊柱损伤占 15.7%,药物使用问题占 43.1%,特别是酒精使用问题占 38.2%。73.5%的病例存在心理健康问题,30.4%的病例曾有自残或自杀企图,37.3%的病例有慢性疼痛。所有病例的巴氯芬血药浓度中位数为3.10毫克/升(第25位0.70毫克/升,第75位8.10毫克/升,范围0.04-110.00毫克/升),无意中毒为1.95毫克/升(第25位0.70毫克/升,第75位4.35毫克/升,范围0.04-24.0毫克/升),有意中毒为6.00毫克/升(第25位1.10毫克/升,第75位13.0毫克/升,范围0.05-110.0毫克/升)。93.8%的患者同时服用药物,其中以抗抑郁药(69.8%)和苯二氮卓类药物(64.6%)最为常见。总之,"典型 "病例为中年人,多数死于蓄意中毒,可能有精神健康和药物使用问题史。我们建议,鉴于巴氯芬有可能被用于蓄意或非蓄意用药过量,因此在开具巴氯芬处方时需要谨慎。
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引用次数: 0
Analyzing 3D facial morphology: Insights from a comparative European and South African study on population affinity, sex, age, and allometry 分析三维面部形态:欧洲和南非人口亲缘关系、性别、年龄和异体测量比较研究的启示
IF 2.2 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2024-10-30 DOI: 10.1016/j.forsciint.2024.112282
Thandolwethu Mbali Mbonani, Ericka Noelle L’Abbé, Alison Fany Ridel
Variable growth patterns and multifactorial mechanisms cause variation in facial shape. These differences in facial morphology pose challenges for craniofacial reconstruction. Three-dimensional (3D) imaging modalities are a valuable resource for examining these variations. In this study, we used geometric morphometric methods to evaluate the effects of population affinity, sex, age, and allometry on the variation and covariation of hard and soft tissue facial morphology matrices in a sample of French and white South African individuals. Seventy-six and 108 cone-beam computed tomography scans of white South African and French nationals, respectively, were retrospectively acquired. Three-dimensional anatomical structures (hard and soft tissue matrices) were extracted using MeVisLab© v. 2.7.1 software for dense landmarking of 43 craniometric, 50 capulometric, and 559 sliding landmarks. Geometric morphometric analyses were used to quantify shape differences attributed to population affinity, sex, age, and allometry and assess the covariation between hard tissue structures and soft facial tissues. Hard and soft tissue facial matrices were influenced by population differences, sexual dimorphism, and aging. Compared to sex and age, population affinity had the strongest influence on variation. In French individuals, all hard and soft tissue matrices were sexually dimorphic, except for the eyes and left external auditory meatus (EAM). In white South Africans, sexual dimorphism was observed for the mouth, midface, and left EAM. Significant shape differences were also observed for specific age groups. The underlying skull and overlying soft tissues were strongly associated with the nose and anterior nasal aperture (correlation, r2-PLS = 0.976), followed by the right ear and right EAM (r2-PLS = 0.875) and the left ear and left EAM (r2-PLS = 0.871) in white South Africans. For French individuals, relatively weak to moderate correlations were observed, and the covariation between matrices was nonsignificant, except for the association between the right ear and right EAM (r2-PLS = 0.499). The smallest covariation was observed between the mouth and midfacial matrix in both populations (South African: r2-PLS = 0.464; French: r2-PLS = 0.367), which was also nonsignificant. This study revealed that 3D imaging technology and geometric morphometric methods can accurately quantify and visualize facial morphology differences. These methods can also evaluate the association between skull structure and soft facial features.
不同的生长模式和多因素机制造成了面部形态的差异。这些面部形态上的差异给颅面重建带来了挑战。三维(3D)成像模式是研究这些差异的宝贵资源。在这项研究中,我们使用几何形态计量学方法评估了法国人和南非白人样本中的人群亲缘关系、性别、年龄和异体测量对面部软硬组织形态矩阵的变化和协变的影响。我们分别对南非白人和法国人进行了 76 次和 108 次锥形束计算机断层扫描。使用 MeVisLab© v. 2.7.1 软件提取了三维解剖结构(硬组织和软组织矩阵),对 43 个颅骨标志、50 个帽状标志和 559 个滑动标志进行了密集标记。几何形态计量分析用于量化因人群亲缘关系、性别、年龄和异体测量而产生的形状差异,并评估硬组织结构与面部软组织之间的协变关系。面部硬组织和软组织矩阵受种群差异、性别二形性和衰老的影响。与性别和年龄相比,人口亲缘关系对变异的影响最大。在法国人中,除眼睛和左外耳道(EAM)外,所有软硬组织基质都具有性别二态性。在南非白人中,口腔、中面部和左外耳道出现了性别二态性。在特定年龄组还观察到明显的形状差异。在南非白人中,下颅骨和上覆软组织与鼻子和前鼻孔密切相关(相关性,r2-PLS = 0.976),其次是右耳和右 EAM(r2-PLS = 0.875)以及左耳和左 EAM(r2-PLS = 0.871)。对于法国人,观察到的相关性相对较弱到中等,矩阵之间的协变不显著,但右耳和右 EAM 之间的相关性除外(r2-PLS = 0.499)。在两种人群中,口腔与中面部矩阵之间的协方差最小(南非人:r2-PLS = 0.464;法国人:r2-PLS = 0.367),但也不显著。这项研究表明,三维成像技术和几何形态计量学方法可以准确量化和直观显示面部形态差异。这些方法还可以评估头骨结构与面部软特征之间的关联。
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Forensic science international
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