Forensic science international最新文献_第4页

Qualitative assessment of DNA isolation from fresh, frozen, and ancient human bone using a homogenizer-assisted workflow 使用均质机辅助工作流程对从新鲜、冷冻和古代人骨中分离的DNA进行定性评估

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-31 DOI: 10.1016/j.forsciint.2025.112807

Volkan Karaman , Fatih Tepgeç , Pulat Akın Sabancı , Ayça Dilruba Aslanger , Mehmet Görgülü , Duygu Dölen , Zehra Oya Uyguner

Effective DNA recovery from bone material is essential for applications in biomedical research, clinical diagnostics, and forensic and archaeogenetic investigations. In this study, DNA isolation performance was evaluated in an exploratory manner in human bone samples representing three preservation states: fresh, cryopreserved, and ancient. All samples were processed using a unified bead mill homogenization and magnetic bead–based extraction workflow in order to maintain procedural consistency. DNA quantity and purity were evaluated by spectrophotometry, and amplifiability was assessed using nuclear and mitochondrial PCR assays as well as representative STR profiling. Fresh and cryopreserved samples yielded higher DNA concentrations and more consistent amplification than ancient specimens, in which recovery was primarily constrained by postmortem degradation. PCR success demonstrated a clear dependence on amplicon length, with shorter mitochondrial and nuclear targets amplifying more reliably across all sample types. Due to the limited sample size and the use of a single individual per preservation group, the results are presented as qualitative observations rather than as statistically generalizable conclusions. Within these constraints, the study demonstrates the feasibility of using a standardized mechanical disruption and extraction workflow across bone samples of differing preservation status and provides a methodological reference for future larger-scale studies involving both modern and degraded skeletal material.

有效地从骨材料中恢复DNA对于生物医学研究、临床诊断、法医和考古调查的应用至关重要。在这项研究中，以探索性的方式评估了代表三种保存状态的人骨样品的DNA分离性能：新鲜，冷冻保存和古代。所有样品均使用统一的球磨均质和基于磁珠的提取工作流程进行处理，以保持程序的一致性。用分光光度法评估DNA的数量和纯度，用核和线粒体PCR分析以及代表性STR分析评估扩增性。新鲜和冷冻保存的样品比古代标本产生更高的DNA浓度和更一致的扩增，古代标本的恢复主要受到死后降解的限制。PCR的成功证明了扩增子长度的明显依赖性，在所有样品类型中，较短的线粒体和核靶标扩增更可靠。由于样本量有限，并且每个保存组使用单个个体，因此结果是定性观察结果，而不是统计上可推广的结论。在这些限制条件下，该研究证明了在不同保存状态的骨样本中使用标准化机械破坏和提取工作流程的可行性，并为未来涉及现代和降解骨骼材料的更大规模研究提供了方法参考。

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引用次数: 0

Technical note MD-VF-Auto SEM: A method of the forensic diatom test that may help end the longstanding “war-on-diatoms” MD-VF-Auto SEM：一种法医硅藻测试方法，可能有助于结束长期的“硅藻战争”。

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-31 DOI: 10.1016/j.forsciint.2025.112803

Sunlin Hu , Liang Chen , Jie Ma , Liguang Li , Yang Ming , Jingjie Wu , Yang Liu , Siyun Guo , Yuchi Zhou

The diatom test has been a classical test for drowning diagnosis for over a century. While it is often regarded in literature as a reliable method—even a golden standard—its validity remains a subject of ongoing controversy. This paper emphasized that the major cause for the controversy is from the methodology of the test. Conventional methods for the test lack sufficient sensitivity and accuracy, making diatom analysis results difficult to be interpreted in both drowning and non-drowning cases. In contrast, the MD-VF-Auto SEM method—which integrates microwave digestion (MD), vacuum filtration (VF), and automated scanning electron microscopy (Auto SEM)—offers significantly improved sensitivity and accuracy. This enhanced method is capable of clarifying the relationship between diatom presence and drowning, thereby addressing key questions regarding the test’s validity. The rationale as well as related research findings are presented. In the end, we call for international collaborative effort to collectively resolve the long-standing “war-on-diatoms”.

一个多世纪以来，硅藻测试一直是诊断溺水的经典测试。虽然它在文学中经常被认为是一种可靠的方法，甚至是黄金标准，但它的有效性仍然是一个持续争议的主题。本文强调，引起争议的主要原因是测试的方法。传统的检测方法缺乏足够的灵敏度和准确性，使得硅藻分析结果在溺水和非溺水情况下都难以解释。相比之下，MD-VF-Auto SEM方法-集成了微波消解（MD），真空过滤（VF）和自动扫描电子显微镜(Auto SEM) -提供了显着提高的灵敏度和准确性。这种增强的方法能够澄清硅藻存在与溺水之间的关系，从而解决有关测试有效性的关键问题。介绍了研究的基本原理和相关研究结果。最后，我们呼吁进行国际合作，共同解决长期存在的“硅藻战争”。

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引用次数: 0

Characterizing bone injuries in avalanche fatalities in the French Alps: Comparing anthropological and surgical classifications 表征在法国阿尔卑斯山雪崩死亡的骨损伤：比较人类学和外科分类

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-31 DOI: 10.1016/j.forsciint.2025.112805

Amandine Fort , Clémence Delteil , Virginie Scolan , Gilbert Ferretti , Emilie Reymond , Marc Blancher , Pascal Adalian

Avalanches are a major cause of death in mountainous regions, primarily from asphyxia. However, increased recreational activities and climate change may be leading to more traumatic injuries, such as bone fractures, which are currently understudied. This study compared two distinct bone fracture classification systems, to better understand specific injury mechanisms in avalanche victims. We conducted a retrospective analysis of post-mortem CT scans from 13 adult avalanche victims in Grenoble, France, all with at least one bone fracture. Using MIP, MPR, and 3D reconstructions, we systematically classified fractures across ten major anatomical regions, representing the entire body. We analyzed each fracture to determine its traumatic mechanism using both the surgical AO/OTA and the anthropological Galloway et al. (2014) classification systems. The study included 13 individuals (61.5 % male; mean age: 37 years), with a total of 265 fractured bones. Fractures were most frequently observed in the thorax (52 %), spine (21 %), and skull (14 %). We found that multiple injury mechanisms, such as impact and compression, often occurred simultaneously. Both classification systems consistently identified six "burst"-type spinal fractures. However, for five open-book pelvic fractures, only the Galloway et al. system precisely described the specific injury mechanism. Our findings indicate that the AO/OTA and Galloway et al. classifications are complementary. The AO/OTA system offers standardized clinical utility, while the Galloway et al. system enhances forensic and anthropological interpretation by elucidating trauma mechanisms. These preliminary insights into bone injury mechanisms in avalanche events emphasize the need for interdisciplinary approaches to improve victim care and safety.

雪崩是山区死亡的主要原因，主要是窒息。然而，娱乐活动的增加和气候变化可能导致更多的创伤性伤害，如骨折，这一点目前还没有得到充分的研究。本研究比较了两种不同的骨折分类系统，以更好地了解雪崩受害者的具体损伤机制。我们对法国格勒诺布尔的13名成年雪崩受害者的死后CT扫描进行了回顾性分析，他们都至少有一处骨折。通过MIP， MPR和3D重建，我们系统地将骨折分为十个主要解剖区域，代表整个身体。我们使用外科AO/OTA和人类学Galloway等人（2014）的分类系统分析了每个骨折，以确定其创伤机制。该研究包括13名个体（61.5% %男性，平均年龄：37岁），共有265块骨折。骨折最常见于胸部（52% %）、脊柱（21% %）和颅骨（14% %）。我们发现多种损伤机制，如撞击和挤压，经常同时发生。两种分类系统一致地确定了六种“爆裂”型脊柱骨折。然而，对于5例开放式骨盆骨折，只有Galloway等人的系统准确描述了具体的损伤机制。我们的研究结果表明，AO/OTA和Galloway等人的分类是互补的。AO/OTA系统提供了标准化的临床应用，而Galloway等人的系统通过阐明创伤机制增强了法医和人类学的解释。这些对雪崩事件中骨损伤机制的初步见解强调了跨学科方法改善受害者护理和安全的必要性。

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引用次数: 0

Temperature-dependent development of Musca domestica (Diptera: Muscidae) and posterior spiracle–based substaging of third instar larvae 家蝇（双翅目：蝇科）的温度依赖性发育和三龄幼虫的后螺旋亚分期

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-30 DOI: 10.1016/j.forsciint.2025.112801

Ziyan Liu , Tae Mo Kang , Jieun Park , Kwang Soo Ko , Young Kyu Park , Seong Hwan Park

Estimating the minimum postmortem interval (mPMI) is an essential task in practical forensic entomology. mPMI estimation is carried out using information on the species recovered at the scene, appropriate developmental data, and the developmental stages of those species. In this study, we obtained developmental data for Musca domestica Linnaeus, 1758 (Diptera: Muscidae), a synanthropic species that inhabits human-associated environments and is also encountered in forensic entomology cases. Musca domestica was reared at constant temperatures ranging from 16 to 34 °C, and we generated developmental data, estimated a thermal summation model, and subdivided third instar development based on time-dependent changes in the posterior spiracle. Musca domestica completed development across all tested temperatures (706.5 h at 16 °C; 163.6 h at 34 °C). For the entire developmental period, the lower developmental threshold and total thermal requirement were estimated as 11.43 °C and 3350.2 accumulated degree hours, respectively. We also documented time-dependent changes in third instar posterior spiracle structures—the peritreme, spiracular plate, slits, and scar—and proposed developmental substages centered on the scar and surrounding morphology. The third instar was subdivided into four substages based on externally observable features of these structures. Through this work, we provide developmental data for Korean populations of the forensically important species M. domestica and propose third instar substages that enable more precise estimation of developmental time and can be applied using simple microscopic observations in forensic casework.

估计最小死后时间是法医昆虫学实践中的一项重要任务。mPMI估计是利用在现场恢复的物种、适当的发育数据和这些物种的发育阶段的信息进行的。本研究获得了Linnaeus家蝇（Musca domestica Linnaeus, 1758）的发育资料（双翅目：蝇科），这是一种生活在人类相关环境中的合生物种，在法医昆虫学案例中也经常遇到。我们将家蝇饲养在16 - 34°C的恒温环境中，生成发育数据，估计一个热总和模型，并根据后气门的时间依赖性变化细分第三期发育。家蝇在所有测试温度下都完成了发育（16°C时为706.5 h； 34°C时为163.6 h）。在整个发育过程中，估计发育下限和总热需求分别为11.43°C和3350.2累积度小时。我们还记录了三龄后气门结构的时间依赖性变化——周膜、气门板、裂口和疤痕——并提出了以疤痕和周围形态为中心的发育亚阶段。根据这些结构的外部可观测特征，将第三阶段细分为四个子阶段。通过这项工作，我们为法医重要物种家蝇的韩国种群提供了发育数据，并提出了第三期亚阶段，可以更精确地估计发育时间，并可以在法医案件中使用简单的显微镜观察。

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引用次数: 0

Use of blood micro-samples in forensic thanatology 微量血液样本在法医病理学中的应用

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-30 DOI: 10.1016/j.forsciint.2025.112798

Myriam Bertrand- Ndoye , Hugo Girard , Florian Hakim , Alexandr Gish , Caroline Ghoul , Yann Delannoy , Quentin Scanvion , Valéry Hédouin , Jean-Michel Gaulier

In forensic thanatology, post-mortem blood is often stored for long periods, leading to in vitro variations in drug concentrations. This study evaluated the use of dried blood microsampling supports dried (Dried Blood Spot – DBS and Volumetric Absorptive MicroSampling (VAMS) as an alternative to conventional tubes. 72 bodies were sampled, including 35 analyzed toxicologically. 6 categories of substances (26 molecules) were quantified using LC-MS/MS or LC-HRMS. Heroin, several benzodiazepines, and cocaine showed excellent stability on DBS, while they degraded or disappeared in conventional tubes. In some cases, the absence of a micro-sample would have prevented the diagnosis of intoxication. For other molecules, concentration differences between DBS and conventional tubes did not alter the medico-legal interpretation, with equivalent values for morphine, codeine, and tramadol. THC and its metabolites remained difficult to extract from DBS. Microsampling has improved the qualitative detection of unstable drugs in post-mortem blood in routine forensic thanalogic sampling practice.

在法医死亡学中，死后的血液通常被储存很长时间，导致体外药物浓度的变化。本研究评估了干血微采样支架（干血斑点- DBS）和体积吸收微采样（VAMS）作为传统管的替代方法的使用。采集了72具尸体样本，其中35具进行了毒理学分析。采用LC-MS/MS或LC-HRMS对6类物质（26个分子）进行定量分析。海洛因、几种苯二氮卓类药物和可卡因在脑起搏器中表现出极好的稳定性，而它们在常规试管中降解或消失。在某些情况下，缺乏微量样本会阻止中毒的诊断。对于其他分子，DBS和传统管之间的浓度差异没有改变医学法律解释，吗啡、可待因和曲马多的浓度相同。四氢大麻酚及其代谢物仍然难以从DBS中提取。显微取样改进了法医常规样品中不稳定药物的定性检测。

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引用次数: 0

A 3D vector-based approach to facial soft tissue–cranial relationships for forensic identification in the Spanish population 三维矢量为基础的方法，面部软组织-颅关系法医鉴定在西班牙人口

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-30 DOI: 10.1016/j.forsciint.2025.112802

Fernando Navarro , Rubén Martos , Sergio Damas , Inmaculada Aleman

Facial soft tissue thickness is a key parameter in forensic craniofacial identification, reflecting the anatomical relationship between the skull and the overlying soft tissues. This study characterizes the spatial correspondence between cranial (craniometric) and facial (cephalometric) landmarks in a Spanish adult population through a three-dimensional vector-based approach applied to computed tomography data. CT scans from 459 healthy individuals (264 males, 195 females; aged 18–60 years) were analyzed. A total set of 12 cephalometric and craniometric landmarks were selected. For each pair of landmarks, the spatial coordinates (X, Y, Z) were extracted and used to compute vectors describing both the orientation and direction in space, as well as the distance corresponding to soft tissue thickness between bone and skin. Statistically significant sex differences were found for most landmarks, but only four exceeded the measurement error threshold. The 3D vectorial approach provides a comprehensive representation of craniofacial spatial relationships beyond linear measurements, allowing a more precise understanding of tissue distribution and landmark orientation. This study presents the first vector-based facial soft tissue thickness reference dataset for the Spanish population, enhancing the accuracy of craniofacial identification and its integration into computational and AI-based forensic applications.

面部软组织厚度是法医颅面鉴定的关键参数，反映了颅骨与上覆软组织的解剖关系。本研究通过应用于计算机断层扫描数据的三维矢量方法，表征了西班牙成年人颅骨（颅测量）和面部（头测量）标志之间的空间对应关系。对459名健康个体（男性264人，女性195人，年龄18-60岁）的CT扫描结果进行了分析。总共选择了12个颅面测量标志。对于每一对地标，提取空间坐标（X， Y， Z）并用于计算描述空间方向和方向的向量，以及骨骼和皮肤之间软组织厚度对应的距离。在大多数地标上发现了统计学上显著的性别差异，但只有四个超过了测量误差阈值。三维矢量方法提供了超越线性测量的颅面空间关系的全面表示，允许更精确地理解组织分布和地标方向。本研究为西班牙人口提供了第一个基于矢量的面部软组织厚度参考数据集，提高了颅面识别的准确性，并将其集成到计算和基于人工智能的法医应用中。

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引用次数: 0

Bloodstain classification methods: What methods do analysts use, why, and how accurate are they? 血迹分类方法：分析人员使用什么方法，为什么使用，准确度如何？

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-30 DOI: 10.1016/j.forsciint.2025.112797

Emma Hook , Sarah Fieldhouse , David Flatman-Fairs , Graham Williams

Many bloodstain pattern classification methods exist in the literature that analysts could use in casework. Currently, no research demonstrates which classification methods bloodstain pattern analysts use or why they use those specific methods; therefore, this study aims to address this gap and support the development of a standardised classification approach. This research surveyed 79 participants working in Bloodstain Pattern Analysis (BPA) to determine which classification methods are used and why. The most used classification methods were the ‘Passive, Spatter, and Altered,’ ‘other methods’ (such as OSAC BPA terminology and Passive, Spatter, Transfer), and ‘Taxonomic methodology,’ and that job role and court system influenced the method chosen. Participants also used their classification methods to classify ten bloodstain patterns. The average percentage of correct classifications was 85 %, consistent with previous research. The percentage of correct classifications was then compared to the classification methods used. No single classification method was shown to be more accurate than any other method for this specific sample. However, as assessing the accuracy and effectiveness of the classification methods was not the main aim of this study, further work is needed to conduct a thorough assessment that will aid in developing a standardised procedure.

文献中存在许多血迹模式分类方法，分析人员可以在案件工作中使用。目前，没有研究表明血迹分析人员使用哪种分类方法或为什么使用这些特定方法；因此，本研究旨在解决这一差距，并支持标准化分类方法的发展。这项研究调查了79名从事血迹模式分析（BPA）工作的参与者，以确定使用哪种分类方法以及原因。最常用的分类方法是“被动、飞溅和改变”、“其他方法”（如OSAC BPA术语和被动、飞溅、转移）和“分类学方法”，工作角色和法院系统影响了所选择的方法。参与者还使用他们的分类方法对十种血迹模式进行分类。正确分类的平均百分比为85 %，与先前的研究一致。然后将正确分类的百分比与使用的分类方法进行比较。对于这一特定样本，没有任何一种分类方法比任何其他方法更准确。然而，由于评估分类方法的准确性和有效性并不是本研究的主要目的，因此需要进一步开展工作来进行彻底的评估，这将有助于制定标准化程序。

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引用次数: 0

Nitazene-related deaths in Norway 2021–2024 2021-2024年挪威nitazene相关死亡人数

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-30 DOI: 10.1016/j.forsciint.2025.112794

Merete Vevelstad , Hege-Merete Krabseth , An-Magritt Haneborg , Per Ole M. Gundersen , Joachim Frost , Åse Marit Leere Øiestad

Nitazenes are potent synthetic opioids that were never marketed as analgesics, due to the high overdose risk with rapid respiratory arrest and death. This study aims to present the nitazene concentrations and toxicological findings in all nitazene-related deaths registered in Norway during 2021–2024. De-identified data from the LIMS database (Oslo University Hospital) and the Beaker database (St. Olavs Hospital) were extracted for all forensic autopsy cases in Norway with nitazenes detected in blood from January 2021 to October 2024, including nitazene and drug concentrations in peripheral blood and urine, age, gender, location of autopsy, date of death, and sampling date. Analysis of nitazenes was performed using liquid chromatography-mass spectrometry methods. Altogether, 36 nitazene-related deaths were registered in Norway; one death in 2021 (etonitazepyne (N-pyrrolidino etonitazene)), one death in 2022 (protonitazene), 13 deaths in 2023 (metonitazene n = 11, metonitazepyne (N-pyrrolidino metonitazene) n = 1, N-desethyl isotonitazene n = 1), and 21 deaths in 2024 (metonitazene n = 18, protonitazepyne (N-pyrrolidino protonitazene) n = 3). The nitazene-related deaths occurred in all regions of Norway, the majority were males (94.4 %), and median age was 29 years (range 19–56 years). Over 90 % of the cases also had one or more of other psychoactive drugs detected in blood, and 50 % had one or more designer benzodiazepines detected. The median (range) concentrations in post-mortem peripheral blood were 7.3 ng/ml (0.8–114.7 ng/ml) for metonitazene (n = 28) and 3.4 ng/ml (2.0–3.7 ng/ml) for protonitazepyne (n = 3). In summary, since June 2023 the prevalence of nitazene-related deaths escalated in Norway, and the majority were males in their twenties or younger. Our findings indicate that inexperienced drug users need better information about the extreme risk of accidental overdose when using such potent opioids.

nitazene是一种强效的合成阿片类药物，从未作为镇痛药上市销售，因为过量使用会导致呼吸骤停和死亡的风险很高。本研究旨在介绍2021-2024年期间挪威登记的所有nitazene相关死亡的nitazene浓度和毒理学结果。从LIMS数据库（奥斯陆大学医院）和Beaker数据库（St. Olavs医院）提取了2021年1月至2024年10月期间挪威所有在血液中检测到nitazene的法医尸检病例的去识别数据，包括外周血和尿液中的nitazene和药物浓度、年龄、性别、尸检地点、死亡日期和采样日期。采用液相色谱-质谱联用法对硝基苯进行分析。在挪威总共登记了36例与硝基苯有关的死亡；一个2021年去世(etonitazepyne (N-pyrrolidino etonitazene)),一个2022年去世(protonitazene), 13人死亡在2023年(metonitazene n = 11日metonitazepyne (N-pyrrolidino metonitazene) n = 1,N-desethyl isotonitazene n = 1),2024年和21例死亡(metonitazene n = 18日protonitazepyne (N-pyrrolidino protonitazene) n = 3)。与nitazene相关的死亡发生在挪威所有地区，大多数为男性（94.4 %），中位年龄为29岁（范围19-56岁）。超过90% %的病例还在血液中检测到一种或多种其他精神活性药物，50% %的病例检测到一种或多种苯二氮卓类药物。死后外周血中metonitazene （n = 28）和protonitazepne （n = 3）的中位浓度（范围）分别为7.3 ng/ml（0.8 ~ 114.7 ng/ml）和3.4 ng/ml（2.0 ~ 3.7 ng/ml）。总而言之，自2023年6月以来，挪威与nitazene有关的死亡流行率上升，大多数是20多岁或更年轻的男性。我们的研究结果表明，没有经验的吸毒者在使用这种强效阿片类药物时需要更好地了解意外过量服用的极端风险。

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引用次数: 0

Comparative performance evaluation of DRI and KIMS immunoassays for forensic drug screening in urine DRI和KIMS免疫测定法在尿液中法医药物筛选中的比较性能评价

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-29 DOI: 10.1016/j.forsciint.2025.112800

Jihyun Kim, Seojin Kang, Nahyun Lee, Siyeon Kim

Rapid and reliable detection of illicit drugs such as methamphetamine and Δ9-tetrahydrocannabinol (THC) is critical in forensic toxicology. Immunoassay-based screening methods offer time-efficient alternatives to instrumental techniques, especially for high-throughput forensic applications. This study aims to comparatively evaluate the analytical performance of two widely used DRI and KIMS immunoassay platforms in detecting these drugs in human urine samples, with a focus on forensic applicability. A total of 866 urine samples were analyzed using both DRI and KIMS-based immunoassays. Key performance metrics such as sensitivity, specificity, cross-reactivity, and cut-off concordance were assessed. All results were compared to mass spectrometry-confirmed reference data. Both platforms demonstrated acceptable performance for preliminary drug screening. However, variations were observed in detection sensitivity for THC and in false-positive rates for methamphetamine. In conclusion, both systems are suitable for high-throughput forensic drug screening. And the findings provide practical guidance for forensic laboratories selecting immunoassay platforms for drugs of abuse screening.

快速和可靠地检测甲基苯丙胺和Δ9-tetrahydrocannabinol（四氢大麻酚）等非法药物对法医毒理学至关重要。基于免疫测定的筛选方法为仪器技术提供了时间效率高的替代方法，特别是对于高通量法医应用。本研究旨在比较评价两种广泛使用的DRI和KIMS免疫分析平台在检测人类尿液样本中这些药物的分析性能，重点是法医适用性。使用DRI和基于kim的免疫测定法分析了总共866份尿液样本。评估关键性能指标，如敏感性、特异性、交叉反应性和切断一致性。将所有结果与质谱确认的参考数据进行比较。两种平台在初步药物筛选中均表现出可接受的性能。然而，四氢大麻酚的检测灵敏度和甲基苯丙胺的假阳性率存在差异。综上所述，这两种系统都适用于高通量法医药物筛选。研究结果可为法医实验室选择免疫分析平台进行药物滥用筛查提供实践指导。

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引用次数: 0

A matter of age: Investigation of the ability to classify epithelial cells of different anatomical origin in aged samples using imaging flow cytometry 年龄问题：使用成像流式细胞术对老年样本中不同解剖来源的上皮细胞进行分类的能力的研究

IF 2.5 3区医学 Q1 MEDICINE, LEGAL

Forensic science international

Pub Date : 2025-12-29 DOI: 10.1016/j.forsciint.2025.112799

Duncan Taylor , Abigail Skeffington , Ishana Somers , Roland A.H. van Oorschot , Giles Best , Mariya Goray

The evolution of forensic biological analysis from identity-focused inquiries to activity-level investigations has necessitated a more nuanced evaluation of biological material sources. In this context, imaging flow cytometry (IFC) has emerged as a promising novel technique for differentiating various epithelial cell types, offering potential advancements in the interpretation of forensic evidence. This project investigates if storage conditions of three and six months had a significant effect on the morphology of four different types of epithelial cells (penile, vaginal, buccal and epidermal) collected from 30 participants (15 males and 15 females) of three different age groups: 18–39, 40–59, and 60 + years. The data collected included 234 morphological measurements collected from over 635 thousand cells that were compared to the fresh samples collected from the same individuals. Our findings show that samples stored for three months, in conditions similar to those expected in forensic casework circumstances, generated similar cell counts (indicating minimal cell loss due to storage and aging) and classification accuracy to samples that were collected and processed immediately. However, the capacity of IFC in sample age identification remains unclear due to the confounding effects of instrument run and sample preparation differences.

法医生物学分析从以身份为中心的调查发展到活动层面的调查，因此有必要对生物材料来源进行更细致入微的评估。在这种背景下，成像流式细胞术（IFC）已成为一种有前途的新技术，用于区分各种上皮细胞类型，为法医证据的解释提供了潜在的进步。该项目研究了3个月和6个月的储存条件是否对4种不同类型上皮细胞（阴茎、阴道、口腔和表皮）的形态有显著影响，这些细胞来自30名参与者（15名男性和15名女性），他们来自3个不同的年龄组：18-39岁、40-59岁和60岁 + 岁。收集的数据包括从63.5万个细胞中收集的234个形态学测量数据，这些数据与从同一个体收集的新鲜样本进行了比较。我们的研究结果表明，在类似于法医案件工作环境的条件下，储存三个月的样本产生了与立即收集和处理的样本相似的细胞计数（表明由于储存和老化导致的细胞损失最小）和分类准确性。然而，由于仪器运行和样品制备差异的混杂影响，IFC在样品年龄鉴定方面的能力仍不清楚。

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引用次数: 0