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Anti-Microbial Resistance of Non-Typhoid Salmonella in Egypt 埃及非伤寒沙门氏菌的耐药性
Pub Date : 2015-11-26 DOI: 10.4172/2167-7972.1000123
Khairy Rmm
Nontyphoidal salmonellosis (NTS) is caused by numerous Salmonella species other than serovars Typhi and Paratyphi, [1]. NTS organisms are common contaminants of food. Poultry meat, eggs, milk, vegetables, and water have been reported as important sources for outbreaks of human Salmonella infection, poultry alone, accounting for up to 50% of salmonellosis outbreaks [2,3].They are also carried by wild animals, rodents, birds, and reptiles, usually with no symptomatic disease [1]. These infections are identified as one of the most common food-borne zoonotic infection in developed and developing countries, but incidence between countries is variable[4]. In Egypt, NTS infections in poultry have increased in the last years [5]. There are excessive uses of antibiotics in zoonotic infections resulting in development of bacterial resistance that can be transmitted to humans by three ways, from direct contact with animals, from the consumption of eggs and meat or through the environment [6]. The resistance to some antibiotics, such as tetracycline, or chloramphenicol is a big challenge for the treatment of children because fluoroquinolons should not be used in this age group [7]. Several studies were performed to determine the prevalence and multidrug resistance of typhoidal salmonella but the existing data about NTS are little in African countries especially in Egypt. Shokry et al., 2015 provides important insight into prevalence and multidrug resistance of NTS in Egypt [8].
非伤寒沙门氏菌病(NTS)是由多种沙门氏菌引起的,而不是伤寒和副伤寒,[1]。NTS生物是食物中常见的污染物。据报道,禽肉、蛋、奶、蔬菜和水是人类沙门氏菌感染暴发的重要来源,仅家禽就占沙门氏菌病暴发的50%[2,3]。野生动物、啮齿动物、鸟类和爬行动物也可携带,通常无症状[1]。这些感染被确定为发达国家和发展中国家最常见的食源性人畜共患感染之一,但各国之间的发病率各不相同[4]。在埃及,NTS在家禽中的感染在过去几年中有所增加[5]。在人畜共患感染中过度使用抗生素,导致细菌产生耐药性,这种耐药性可通过三种途径传播给人类,即与动物直接接触、食用蛋类和肉类或通过环境传播[6]。对某些抗生素的耐药性,如四环素或氯霉素,对儿童的治疗是一个很大的挑战,因为氟喹诺酮类药物不应该在这个年龄组使用[7]。为确定伤寒沙门氏菌的流行和多药耐药性进行了几项研究,但在非洲国家,特别是在埃及,关于NTS的现有数据很少。Shokry等人,2015年为NTS在埃及的流行和多药耐药提供了重要见解[8]。
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引用次数: 4
The Role of Aerobic Microorganisms in the Biodegradation of PetroleumHydrocarbons Laboratory Contaminated Groundwater 好氧微生物在石油烃实验室污染地下水生物降解中的作用
Pub Date : 2015-11-18 DOI: 10.4172/2167-7972.1000122
A. Owhonka, Gideon Oa
The total hydrocarbon content removal in nutrient amendment and natural attenuation at day 56 showed percentage removal of 89 and 74, respectively. Total heterotrophic and total hydrocarbon utilizing bacterial count increased progressively in all the treatment options. The nutrient amendment showed greater removal of the hydrocarbon pollutants. The physicochemical analyses at day 56 were within the World Health Organization standard for drinking water.
在第56天,营养修正法和自然衰减法对总烃的去除率分别为89%和74%。在所有处理方案中,总异养菌数和总烃类利用菌数逐渐增加。营养改性对烃类污染物的去除效果较好。第56天的理化分析结果符合世界卫生组织的饮用水标准。
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引用次数: 3
Distribution of a kokumi peptide, γ-Glu-Val-Gly, in various fermented foods andthe possibility of its contribution to the sensory quality of fermented foods kokumi肽γ-Glu-Val-Gly在各种发酵食品中的分布及其对发酵食品感官质量贡献的可能性
Pub Date : 2015-11-05 DOI: 10.4172/2167-7972.1000121
Naohiro Miyamura, M. Kuroda, T. Mizukoshi, Y. Kato, J. Yamazaki, H. Miyano, Y. Eto
A potent kokumi peptide, γ-glutamyl-valyl-glycine (γ-Glu-Val-Gly), was identified and quantified in various fermented foods using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). This peptide was present in fermented seasoning such as fish sauces, soy sauces, and fermented shrimp pastes. Among the brewed alcoholic beverages, γ-Glu-Val-Gly was detected in beer. In addition, the content of γ-Glu-Val-Gly positively correlated with the quality grade of dark-colored soy sauce (correlation coefficient ρ=0.810, p<0.05). These results suggest that γ-Glu-Val-Gly is widely distributed in fermented foods and that the content of this peptide can be an indicator of sensory quality of fermented foods.
采用高效液相色谱-串联质谱(LC/MS/MS)技术对发酵食品中的γ-谷氨酰基谷氨酸(γ-Glu-Val-Gly)进行了鉴定和定量。这种肽存在于发酵调味料中,如鱼酱、酱油和发酵虾酱。在酿造酒精饮料中,啤酒中检测到γ-Glu-Val-Gly。γ-Glu-Val-Gly含量与酱油质量等级呈正相关(相关系数ρ=0.810, p<0.05)。这些结果表明,γ-Glu-Val-Gly在发酵食品中分布广泛,其含量可以作为发酵食品感官质量的一个指标。
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引用次数: 10
Phytosterol bioconversions by Actinobacteria and their application in steroid pharmaceutical industry 放线菌对植物甾醇的生物转化及其在类固醇制药工业中的应用
Pub Date : 2015-09-29 DOI: 10.4172/2167-7972.S1.001
M. Donova
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引用次数: 0
Bacteriophages: New class of beneficial microbes and their practical applications 噬菌体:一类新的有益微生物及其实际应用
Pub Date : 2015-09-29 DOI: 10.4172/2167-7972.S1.002
Àlex, er Sulakvelidze
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引用次数: 0
Antimicrobial activity and properties of Enterococcus faecium strains isolated from kefir 开菲尔菌中粪肠球菌的抑菌活性及特性研究
Pub Date : 2015-09-29 DOI: 10.4172/2167-7972.S1.003
Erdogan Cakir Merih Kivanc, Tülay Yiğit
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引用次数: 16
Effect of Nutrient Supply on the Production of Soluble Microbial Products(SMP) in Anaerobic Reactors 厌氧反应器中营养物供给对可溶性微生物产物(SMP)产生的影响
Pub Date : 2015-09-16 DOI: 10.4172/2167-7972.1000120
Weili Zhou, Bingtao Wu, Xiaojing Dong, Shengbing He
Three up-flow anaerobic sludge blanket (UASB) reactors added with different amount of nutrients were compared to investigate the effect of nutrient on the production of soluble microbial products (SMP) in the bench-scale anaerobic reactors. The running performance and the analysis of SMP verified that the nutrient level could greatly affect the treatment performance and the quality of anaerobic effluent. The surplus nutrimental addition would stimulate the bacterial activity and increase the metabolism products, causing the high concentration of effluent organic matter. On the contrary, the nutrient deficiency could keep the microbial activity at a low level, which caused the temporary better anaerobic effluent at the low organic loading rate (OLR), but might lead to a vulnerable microbial system, even system failure when OLR went higher. Molecular analysis revealed that the abundant existence of Methanosaeta might be beneficial to the SMP consumption.
通过对3个上流式厌氧污泥毯(UASB)反应器中添加不同营养物质的比较,研究了营养物质对反应器中可溶性微生物产物(SMP)产量的影响。SMP的运行性能和分析表明,营养物水平对厌氧出水的处理性能和水质有很大影响。过量的营养添加会刺激细菌活性,增加代谢产物,导致出水有机物浓度高。相反,营养缺乏可以使微生物活性保持在较低水平,在低有机负荷率(OLR)下暂时获得较好的厌氧出水,但当OLR更高时,可能导致微生物系统脆弱,甚至系统失效。分子分析表明,甲烷菌的大量存在可能有利于SMP的消耗。
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引用次数: 1
Comments on Antituberculosis Activity of Bioactive Compounds from Acacia nilotica 金合欢生物活性化合物抗结核活性研究进展
Pub Date : 2015-08-27 DOI: 10.4172/2167-7972.1000118
P. Oladosu
TB is a treatable and curable disease. Active drug-sensitive TB disease is treated with a standard six-month course of four antimicrobial drugs that are provided with information, supervision and support to the patient by a health worker or trained volunteer. Without such supervision and support, treatment adherence can be difficult and the disease can spread. The vast majority of TB cases can be cured when medicines are provided and taken properly.
结核病是一种可治疗和可治愈的疾病。活动性药物敏感结核病的治疗采用标准的六个月疗程,使用四种抗微生物药物,由卫生工作者或经过培训的志愿者向患者提供信息、监督和支持。如果没有这种监督和支持,坚持治疗可能会很困难,而且疾病可能会传播。只要提供药物并正确服用,绝大多数结核病病例都可以治愈。
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引用次数: 1
Optimization of Cultural Parameters for Cost Effective Production of Kojic Acid by Fungal Species Isolated from Soil 土壤分离真菌高效生产曲酸的培养参数优化
Pub Date : 2015-08-27 DOI: 10.4172/2167-7972.1000119
Durgadevi Kb, P. Vijayalakshmi, Shilpa, Kumar Bv
A novel isolate capable of producing opulent yields of kojic acid with surface fermentation was screened from ten different isolated soil fungi. The organism was confirmed as Aspergillus flavus FJ537130 strain using 18SrRNA based molecular analysis and it was identified as a negative producer of aflatoxin. Though twelve different carbon sources were used, significant yields of kojic acid crystals was noticed with Sago starch. The optimized conditions established were substrate concentration 1000 ml (100 g of starch powder in 1000 ml of H2O), pH 6.0, Time 28 d, Temperature 28°C, Peptone concentration 4 g/l, KH2PO4 concentration 1 g/l, MgSO4 concentration 0.5 g/l and the yield achieved was 28.5 g/l. The resulted fermentation broth was subjected to solvent extraction followed by gel filtration for the separation of kojic acid and crystallization. The structural characterization of purified kojic acid was confirmed by Proton NMR, FTIR and XRD. The molecular weight and purity of kojic acid was confirmed by LC/MS and HPLC. The isolated kojic acid crystals shows high antimicrobial activity against pathogenic bacteria Staphylococcus aureus and Escherichia coli and maximum zone of inhibition was 9 mm. The inhibitory effect of kojic acid was more on the cell line K562 (Leukemia) when compared to the MDAMB435S (Breast cancer) cell line.
从10种不同的土壤分离真菌中筛选出一株表面发酵产曲酸的新型分离菌。基于18SrRNA的分子分析证实该菌株为黄曲霉FJ537130,为黄曲霉毒素阴性菌株。虽然使用了12种不同的碳源,但西米淀粉的曲酸晶体产量显著。优化条件为底物浓度1000 ml (100 g淀粉粉加入1000 ml H2O中),pH 6.0,时间28 d,温度28℃,蛋白胨浓度4 g/l, KH2PO4浓度1 g/l, MgSO4浓度0.5 g/l,产率为28.5 g/l。所得发酵液经溶剂萃取、凝胶过滤分离曲酸和结晶。通过质子核磁共振、红外光谱和x射线衍射对纯化的曲酸进行了结构表征。采用液相色谱/质谱和高效液相色谱法确定了曲酸的分子量和纯度。分离得到的曲酸晶体对病原菌金黄色葡萄球菌和大肠杆菌具有较高的抑菌活性,最大抑菌区为9 mm。与MDAMB435S(乳腺癌)细胞株相比,曲酸对K562(白血病)细胞株的抑制作用更大。
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引用次数: 3
Volatile Composition of Wines Elaborated from Organic and Non-OrganicGrapes 有机和非有机葡萄酿造葡萄酒的挥发性成分
Pub Date : 2015-08-21 DOI: 10.4172/2167-7972.1000117
C. Lorenzo, T. Garde-Cerdán, José Félix Lara, Martínez-Gi Am, F. Pardo, M. R. Salinas
The aim of this work was to study the evolution of volatile compounds during the alcoholic fermentation of organic and non-organic grapes. To do this, grapes were cultivated using organic and non-organic grapes; their tasting showed some differences between both types of Monastrell grapes. Throughout the alcoholic fermentation, the samples of organic grapes had higher concentration of total alcohols but lower concentration of esters and acids than the samples of conventional ones. Therefore, the volatile composition of wines from two different cultivated grapes using both agronomic practices was different. Moreover, regarding to the volatile compounds that contributed directly to wine aroma, generally organic wine had more chemicals and floral aromas, while the wines from conventional practices had more fruity aromas. Principal component analysis (PCA) showed that it is possible to differentiate between both types of wines in terms of concentration of volatile compounds formed during the alcoholic fermentation. Consequently, agronomic practices affected the grape taste, the wine volatile composition and its quality.
本研究的目的是研究有机和非有机葡萄在酒精发酵过程中挥发性化合物的演变。为了做到这一点,葡萄种植使用有机和非有机葡萄;他们的品尝结果显示出两种莫纳斯特雷尔葡萄之间的一些差异。在整个酒精发酵过程中,有机葡萄样品的总醇浓度高于常规葡萄样品,而酯类和酸类浓度低于常规葡萄样品。因此,用两种不同的农艺方法栽培的葡萄酿造的葡萄酒的挥发性成分是不同的。此外,就直接影响葡萄酒香气的挥发性化合物而言,有机葡萄酒通常含有更多的化学物质和花香,而传统做法的葡萄酒则含有更多的水果香气。主成分分析(PCA)表明,根据酒精发酵过程中形成的挥发性化合物的浓度,可以区分两种类型的葡萄酒。因此,农艺做法影响了葡萄的味道、葡萄酒的挥发性成分和品质。
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引用次数: 3
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Fermentation Technology
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