Pub Date : 2015-08-09DOI: 10.4172/2167-7972.1000116
Miwa Yamada, K. Isobe
Glycolic acid is an attractive raw material which is used as a dyeing and tanning agent in the textile industry, a flavoring agent and preservative in the food processing industry, and a skin care agent in the pharmaceutical industry. It is also utilized for the production of polyglycolic acid and other biocompatible copolymers. Glycolic acid can be isolated from natural sources, such as sugarcane, sugar beets, pineapple, or cantaloupe, but it is also chemically synthesized by hydrogenation of oxalic acid with nascent hydrogen or the hydrolysis of the cyanohydrin derived from formaldehyde. Ethylene glycol is a relatively inexpensive starting material for the production of glycolic acid by an oxidation reaction. However, the chemical oxidation reaction of ethylene glycol has certain drawbacks, such as the formation of formaldehyde and other compounds as by-products. To overcome such drawbacks of chemical synthesis for the production of glycolic acid, one of the preferred methods is to use enzymatic production rather than chemical synthesis. The utilization of microbial enzymes also has the major advantage of promoting simple and eco-friendly industrial-scale production. We therefore designed a new enzymatic method for the production of glycolic acid from ethylene glycol using two microbial oxidases; ethylene glycol is first converted to glycolaldehyde by an ethylene glycol-oxidizing enzyme, and the resulting glycolaldehyde is then oxidized to glycolic acid by an aldehyde oxidase (ALOD) (Figure 1).
{"title":"A Novel Microbial Aldehyde Oxidase Applicable to Production of Useful Raw Materials, Glycolic Acid and Glyoxylic Acid, from Ethylene Glycol","authors":"Miwa Yamada, K. Isobe","doi":"10.4172/2167-7972.1000116","DOIUrl":"https://doi.org/10.4172/2167-7972.1000116","url":null,"abstract":"Glycolic acid is an attractive raw material which is used as a dyeing and tanning agent in the textile industry, a flavoring agent and preservative in the food processing industry, and a skin care agent in the pharmaceutical industry. It is also utilized for the production of polyglycolic acid and other biocompatible copolymers. Glycolic acid can be isolated from natural sources, such as sugarcane, sugar beets, pineapple, or cantaloupe, but it is also chemically synthesized by hydrogenation of oxalic acid with nascent hydrogen or the hydrolysis of the cyanohydrin derived from formaldehyde. Ethylene glycol is a relatively inexpensive starting material for the production of glycolic acid by an oxidation reaction. However, the chemical oxidation reaction of ethylene glycol has certain drawbacks, such as the formation of formaldehyde and other compounds as by-products. To overcome such drawbacks of chemical synthesis for the production of glycolic acid, one of the preferred methods is to use enzymatic production rather than chemical synthesis. The utilization of microbial enzymes also has the major advantage of promoting simple and eco-friendly industrial-scale production. We therefore designed a new enzymatic method for the production of glycolic acid from ethylene glycol using two microbial oxidases; ethylene glycol is first converted to glycolaldehyde by an ethylene glycol-oxidizing enzyme, and the resulting glycolaldehyde is then oxidized to glycolic acid by an aldehyde oxidase (ALOD) (Figure 1).","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83211672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-18DOI: 10.4172/2167-7972.1000E122
S. Oda
A Liquid–liquid interface bioreactor (L–L IBR), which consists of a hydrophobic organic solvent (an upper phase), a layer of fungal cellballooned polyacrylonitrile microsphere (diameter, 20-40 μm; density, 0.03-0.20; a middle phase), and a liquid medium (a lower phase), is a unique and effective cultivation system for the microbial transformation with fungi [1]. The system has some interesting and practically important characteristics, i.e., alleviation of toxicity of poisonous substrate and/or product solubilized in the organic phase, excellent productivity of valuable hydrophobic chemicals, efficient supply of oxygen from the organic phase to fungal cells, control and management of pH and nutrients in the liquid medium, depression of catabolize repression caused by easily metabolizable carbon sources [2], and easy recovery of product without troublesome solvent extraction. The system has been applied to various microbial reactions, such as hydrolysis of an acetate ester [1,3], asymmetric reduction of an aromatic dike tone [4], and regio- and stereo selective epoxidation of - caryophyllene to (–)-β-caryophyllene oxide so far [5]. In all cases, it has been observed that substrate concentration, product accumulation, and region- and stereo selectivity’s of reaction reached very high level compared with two traditional cultivation systems, submerged and organic–aqueous two-liquid-phase systems.
{"title":"Relationship between Interfacial Hydrophobicity and Hydroxylation Activity ofFungal Cells Located on an Organic Aqueous Interface","authors":"S. Oda","doi":"10.4172/2167-7972.1000E122","DOIUrl":"https://doi.org/10.4172/2167-7972.1000E122","url":null,"abstract":"A Liquid–liquid interface bioreactor (L–L IBR), which consists of a hydrophobic organic solvent (an upper phase), a layer of fungal cellballooned polyacrylonitrile microsphere (diameter, 20-40 μm; density, 0.03-0.20; a middle phase), and a liquid medium (a lower phase), is a unique and effective cultivation system for the microbial transformation with fungi [1]. The system has some interesting and practically important characteristics, i.e., alleviation of toxicity of poisonous substrate and/or product solubilized in the organic phase, excellent productivity of valuable hydrophobic chemicals, efficient supply of oxygen from the organic phase to fungal cells, control and management of pH and nutrients in the liquid medium, depression of catabolize repression caused by easily metabolizable carbon sources [2], and easy recovery of product without troublesome solvent extraction. The system has been applied to various microbial reactions, such as hydrolysis of an acetate ester [1,3], asymmetric reduction of an aromatic dike tone [4], and regio- and stereo selective epoxidation of - caryophyllene to (–)-β-caryophyllene oxide so far [5]. In all cases, it has been observed that substrate concentration, product accumulation, and region- and stereo selectivity’s of reaction reached very high level compared with two traditional cultivation systems, submerged and organic–aqueous two-liquid-phase systems.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"19 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2015-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84085837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-04-08DOI: 10.4172/2167-7972.1000113
Philippe Mambanzulua Ngoma, S. Hiligsmann, Eric Sumbu Zola, M. Ongena, P. Thonart
Vegetal waste and some wastewater of agro-food industries contain plant secondary metabolites (PSMs). It was showed in nutritional researches that these substances such as saponins and tannins reduced the methane production in the rumen. To our knowledge no study was done in the waste treatment domain to evaluate the inhibitory effect of the principal glycosidic metabolites from the wastewater or vegetal waste on their own methaneproducing anaerobic digestion. Therefore in this paper BMP tests were carried out at 30°C with four commercial PSMs (CPSMs) in mixture with glucose monohydrate (Gl) used as control sample. These CPSMs were saponin from Quilaja saponaria Molina Pract (Sap), tannic acid (Tan), salicin (Sal) and aloin from Curacao Aloe (Alo) representing respectively saponins, tannins, alcoholic glycosides and anthraquinones sources. Acidogenesis and acetogenesis were recorded for all the mixtures of Gl and CPSMs; however their conversion rates decreased with the increase of the concentrations of CPSMs. By contrast, the methanogenesis was inhibited at concentrations of CPSMs above 0.3 g/l. The inhibition degree for aromatic compounds on the anaerobic biodegradation of Gl seemed directly to depend on the numbers of benzene rings in the medium and the synergism. Thus, the highest inhibition of the biogas production from Gl was recorded for Alo, followed by Sap, Tan and Sal. However, the highest inhibition of the methane production from Gl was recorded with Sap, Alo, Tan and Sal. It was supposed that the toxicity potentials of these PSMs on their own biomethanization would be in following decreasing order: Sap or Alo, Tan and Sal. Therefore, the concentration of PSMs alone or in mixture in a digester should be below 0.3 g/l for a better methanization.
{"title":"Impact of Different Plant Secondary Metabolites Addition: Saponin, Tannic Acid, Salicin and Aloin on Glucose Anaerobic Co-Digestion","authors":"Philippe Mambanzulua Ngoma, S. Hiligsmann, Eric Sumbu Zola, M. Ongena, P. Thonart","doi":"10.4172/2167-7972.1000113","DOIUrl":"https://doi.org/10.4172/2167-7972.1000113","url":null,"abstract":"Vegetal waste and some wastewater of agro-food industries contain plant secondary metabolites (PSMs). It was showed in nutritional researches that these substances such as saponins and tannins reduced the methane production in the rumen. To our knowledge no study was done in the waste treatment domain to evaluate the inhibitory effect of the principal glycosidic metabolites from the wastewater or vegetal waste on their own methaneproducing anaerobic digestion. Therefore in this paper BMP tests were carried out at 30°C with four commercial PSMs (CPSMs) in mixture with glucose monohydrate (Gl) used as control sample. These CPSMs were saponin from Quilaja saponaria Molina Pract (Sap), tannic acid (Tan), salicin (Sal) and aloin from Curacao Aloe (Alo) representing respectively saponins, tannins, alcoholic glycosides and anthraquinones sources. Acidogenesis and acetogenesis were recorded for all the mixtures of Gl and CPSMs; however their conversion rates decreased with the increase of the concentrations of CPSMs. By contrast, the methanogenesis was inhibited at concentrations of CPSMs above 0.3 g/l. The inhibition degree for aromatic compounds on the anaerobic biodegradation of Gl seemed directly to depend on the numbers of benzene rings in the medium and the synergism. Thus, the highest inhibition of the biogas production from Gl was recorded for Alo, followed by Sap, Tan and Sal. However, the highest inhibition of the methane production from Gl was recorded with Sap, Alo, Tan and Sal. It was supposed that the toxicity potentials of these PSMs on their own biomethanization would be in following decreasing order: Sap or Alo, Tan and Sal. Therefore, the concentration of PSMs alone or in mixture in a digester should be below 0.3 g/l for a better methanization.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"67 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2015-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74554086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-04-08DOI: 10.4172/2167-7972.1000115
R. Hewawasam, C. Udawatte, S. Weliwegamage, S. Sotheeswaran, S. Rajapakse
Immobilization of macro molecules (such as enzymes) and micro-organisms can be generally defined as a procedure leading to their restricted mobility. Advantages of immobilization include easy separation of the enzymes/cells from the product and reuse of the enzymes/cells. In this research coconut tree leaf sheath was used to immobilize selected microbes which were used in fermentation tecnology. Coconut tree leaf sheath contains cellulose fiber layers which have cross linking between them. Sacchromyces cerevisiae was used as the microbial type due to widespread use in fermentation process. Microbes were entrapped within cellulose layers. Coconut tree leaf sheath was found to be an effecient solid support for immobilization. Immobilized microbes can be reused for fresh fermentation media. Immobilization can be carried out utilizing naturally avavilable coconut tree leaf sheath as a solid support, it`s usage is very cost-effective and eco-froiendly method rather than using synthetic or semi synthetic solid supports.
{"title":"Immobilization of Selected Microbes at Some Selected Solid Supports for Enhanced Fermentation Process","authors":"R. Hewawasam, C. Udawatte, S. Weliwegamage, S. Sotheeswaran, S. Rajapakse","doi":"10.4172/2167-7972.1000115","DOIUrl":"https://doi.org/10.4172/2167-7972.1000115","url":null,"abstract":"Immobilization of macro molecules (such as enzymes) and micro-organisms can be generally defined as a procedure leading to their restricted mobility. Advantages of immobilization include easy separation of the enzymes/cells from the product and reuse of the enzymes/cells. In this research coconut tree leaf sheath was used to immobilize selected microbes which were used in fermentation tecnology. Coconut tree leaf sheath contains cellulose fiber layers which have cross linking between them. Sacchromyces cerevisiae was used as the microbial type due to widespread use in fermentation process. Microbes were entrapped within cellulose layers. Coconut tree leaf sheath was found to be an effecient solid support for immobilization. Immobilized microbes can be reused for fresh fermentation media. Immobilization can be carried out utilizing naturally avavilable coconut tree leaf sheath as a solid support, it`s usage is very cost-effective and eco-froiendly method rather than using synthetic or semi synthetic solid supports.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"341 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2015-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72855829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.4172/2167-7972.1000112
Huda Fatima, N. Khan, A. Rehman, Z. Hussain
The production of lipase from Pseudomonas putida 922 was optimized by modifying various physical parameters such as carbon source, nitrogen source, pH, salt concentration and biochemical parameters of the production medium such as temperature and incubation time of the growth medium. Oil cakes were also used as carbon source to check for an increased production of the enzyme. The bacterium was found to have a maximal growth at pH 10 with the enzyme production being highest (24 U/ml) after 48 hours at 30°C and pH 10. The optimum composition of the medium was mustard oil cake as carbon source, yeast extract or peptone as nitrogen source and 1% sodium chloride concentration. Partial characterization of the enzyme was carried out where the optimum working pH and temperature was found to be 10 and 40oC, respectively. Enzyme stability was found to lie in the pH and temperature ranges of 5-11 and 30-40oC, respectively. Partial purification of the enzyme was carried out at 80% ammonium sulphate saturation. Molecular mass of lipase was determined by SDS PAGE and found to be 45 kDa.
{"title":"Production and Partial Characterization of Lipase from Pseudomonas putida","authors":"Huda Fatima, N. Khan, A. Rehman, Z. Hussain","doi":"10.4172/2167-7972.1000112","DOIUrl":"https://doi.org/10.4172/2167-7972.1000112","url":null,"abstract":"The production of lipase from Pseudomonas putida 922 was optimized by modifying various physical parameters such as carbon source, nitrogen source, pH, salt concentration and biochemical parameters of the production medium such as temperature and incubation time of the growth medium. Oil cakes were also used as carbon source to check for an increased production of the enzyme. The bacterium was found to have a maximal growth at pH 10 with the enzyme production being highest (24 U/ml) after 48 hours at 30°C and pH 10. The optimum composition of the medium was mustard oil cake as carbon source, yeast extract or peptone as nitrogen source and 1% sodium chloride concentration. Partial characterization of the enzyme was carried out where the optimum working pH and temperature was found to be 10 and 40oC, respectively. Enzyme stability was found to lie in the pH and temperature ranges of 5-11 and 30-40oC, respectively. Partial purification of the enzyme was carried out at 80% ammonium sulphate saturation. Molecular mass of lipase was determined by SDS PAGE and found to be 45 kDa.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"83 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81805220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-09-16DOI: 10.4172/2167-7972.1000E117
M. Camassola
Mushrooms are fungi belonging to the higher phyla Ascomycota and Basidiomycota. In fact the name mushroom refers to a fruiting body, formed by several hyphae that grow upwards and produces spores (basidiospores). These spores are invisible to the naked eye and spread with the wind, with water or even attached to the body of animals. The fruiting fungus is the structure of sexual reproduction and has different shapes and colors.
{"title":"Mushrooms-The Incredible Factory for Enzymes and Metabolites Productions","authors":"M. Camassola","doi":"10.4172/2167-7972.1000E117","DOIUrl":"https://doi.org/10.4172/2167-7972.1000E117","url":null,"abstract":"Mushrooms are fungi belonging to the higher phyla Ascomycota and Basidiomycota. In fact the name mushroom refers to a fruiting body, formed by several hyphae that grow upwards and produces spores (basidiospores). These spores are invisible to the naked eye and spread with the wind, with water or even attached to the body of animals. The fruiting fungus is the structure of sexual reproduction and has different shapes and colors.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"268 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2013-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79835578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-03-16DOI: 10.4172/2167-7972.1000110
Pradipta Tokdar, A. Wani, Pratyush Kumar, Prafull V. Ranadive, S. George
Viscous nature of the fermentation broth has phenomenal influence on process conditions and parameters in a fermentor. Though broth rheology has attracted significant influence in process research, still there is a challenge to modify fluid dynamics of fermentation broth. During the production of coenzymeQ10 (CoQ10) by Agrobacterium tumefaciens ATCC 4452, the culture broth becomes highly viscous due to excessive synthesis of exopolysaccharides. This hinders the CoQ10 yield and complicates the downstream process. The present study describes how this problem was tackled by media modification and mutation. Induced mutants were generated using UV and EMS as mutagenic agents followed by rational selection based on antibiotic resistance. On screening of these mutants in sucrose based PM-2 medium, UV induced, vancomycin resistant mutant M-6, showed significant reduction (6.29 fold) in viscosity development in the broth. Mutant M-6(S), a natural variant of mutant M-6, resistant to high substrate concentration was further selected for the CoQ10 production. Cane molasses as carbon source was found to be best suitable for CoQ10 fermentation using mutant M-6(S). Replacing sucrose with cheaper cane molasses significantly reduced the broth viscosity with improved specific CoQ10 content, thereby generating cost effective fermentation process. The newly developed mutant strain produced 48.89 mg/L of CoQ10 with specific CoQ10 content of 1.87 mg/g of DCW at 25°C, 500 rpm agitation and 0.2 vvm aeration using continuous fed batch fermentation and newly formulated cane molasses medium.
{"title":"Process and Strain Development for Reduction of Broth Viscosity with Improved Yield in Coenzyme Q10 Fermentation by Agrobacterium tumefaciens ATCC 4452","authors":"Pradipta Tokdar, A. Wani, Pratyush Kumar, Prafull V. Ranadive, S. George","doi":"10.4172/2167-7972.1000110","DOIUrl":"https://doi.org/10.4172/2167-7972.1000110","url":null,"abstract":"Viscous nature of the fermentation broth has phenomenal influence on process conditions and parameters in a fermentor. Though broth rheology has attracted significant influence in process research, still there is a challenge to modify fluid dynamics of fermentation broth. During the production of coenzymeQ10 (CoQ10) by Agrobacterium tumefaciens ATCC 4452, the culture broth becomes highly viscous due to excessive synthesis of exopolysaccharides. This hinders the CoQ10 yield and complicates the downstream process. The present study describes how this problem was tackled by media modification and mutation. Induced mutants were generated using UV and EMS as mutagenic agents followed by rational selection based on antibiotic resistance. On screening of these mutants in sucrose based PM-2 medium, UV induced, vancomycin resistant mutant M-6, showed significant reduction (6.29 fold) in viscosity development in the broth. Mutant M-6(S), a natural variant of mutant M-6, resistant to high substrate concentration was further selected for the CoQ10 production. Cane molasses as carbon source was found to be best suitable for CoQ10 fermentation using mutant M-6(S). Replacing sucrose with cheaper cane molasses significantly reduced the broth viscosity with improved specific CoQ10 content, thereby generating cost effective fermentation process. The newly developed mutant strain produced 48.89 mg/L of CoQ10 with specific CoQ10 content of 1.87 mg/g of DCW at 25°C, 500 rpm agitation and 0.2 vvm aeration using continuous fed batch fermentation and newly formulated cane molasses medium.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"47 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78396680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.4172/2167-7972.1000109
J. Manwar, K. Mahadik, A. Paradkar
Plackett-Burman design, a statistical method was successfully employed for the optimization of the fermentation process for the yeast Saccharomyces cerevisiae. The yeast used in the study was isolated from the flowers of Woodfordia fruticosa in our previous work. The effect of simultaneously varying the jaggery concentration, inoculums volume and incubation temperature on alcohol yield was studied with the help of the response surface methodology. The optimum conditions found were jaggery (40% w/v), inoculums volume (8% v/v) and temperature (30°C). After process optimization, alcohol yield was increased from 69.57 g/l to 95.84 g/l. The results demonstrated that the strain S. cerevisiae could be used efficiently as inoculums for the fermentation process at optimal conditions.
{"title":"Plackett-Burman Design: A Statistical Method for the Optimization of Fermentation Process for the Yeast Saccharomyces cerevisiae Isolated from the Flowers of Woodfordia fruticosa","authors":"J. Manwar, K. Mahadik, A. Paradkar","doi":"10.4172/2167-7972.1000109","DOIUrl":"https://doi.org/10.4172/2167-7972.1000109","url":null,"abstract":"Plackett-Burman design, a statistical method was successfully employed for the optimization of the fermentation process for the yeast Saccharomyces cerevisiae. The yeast used in the study was isolated from the flowers of Woodfordia fruticosa in our previous work. The effect of simultaneously varying the jaggery concentration, inoculums volume and incubation temperature on alcohol yield was studied with the help of the response surface methodology. The optimum conditions found were jaggery (40% w/v), inoculums volume (8% v/v) and temperature (30°C). After process optimization, alcohol yield was increased from 69.57 g/l to 95.84 g/l. The results demonstrated that the strain S. cerevisiae could be used efficiently as inoculums for the fermentation process at optimal conditions.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"333 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80573291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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