Zhiyu Zhao, Kevin Kantono, Rothman Kam, Thao T Le, Eileen Kitundu, Tony Chen, Nazimah Hamid
Apricot kernels are known for being a rich source of oil, protein, and bioactive compounds. This study focused on enhancing the bioactivities of apricot kernels through fermentation. Additionally, this study explored the correlations between polyphenols, amino acids, antioxidant activities, and total phenolic content (TPC). The findings indicated that apricot kernels fermented with Lactiplantibacillus plantarum exhibited increased antioxidant activity, as assessed by the FRAP and CUPRAC methods, and an increased TPC compared to naturally fermented samples. The CUPRAC activity increased significantly from 1.03 to 1.82 mg of ascorbic acid per gram of sample on day 7, and the FRAP activity increased from 4.9 to 12.2 mg of ascorbic acid per gram of sample on day 3 of fermentation. Moreover, the TPC significantly increased from 1.67 to 7.58 mg of gallic acid per gram of sample on day 9 of fermentation. The results further demonstrated that, during the fermentation process, the concentration of hydroxybenzoic acid increased from 0.52 µg/g on day 0 to 5.3 µg/g on day 9. The DL-3-phenyllactic acid content demonstrated a significant increase from 0.42 µg/g on day 0 to 99.62 µg/g on day 5, while the benzoic acid content exhibited a notable increase from 45.33 µg/g to 138.13 µg/g over the fermentation period, with peak levels being observed on day 5. Similarly, most amino acids demonstrated a rise in concentration as the fermentation progressed, peaking on the ninth day. This study further employed random forest regression as a form of explainable artificial intelligence (XAI) to explore the relationships between phenolic compounds, amino acids, and antioxidant activities. Amino acids like L-cystine and L-anserine were found to positively impact FRAP values, while L-histidine and 1-methyl-L-histidine contributed to the CUPRAC antioxidant activity. Notably, hydroxybenzoic acid emerged as a key contributor to both the FRAP value and TPC, highlighting its significance in improving the overall antioxidant capacity of apricot kernels. These findings indicate that, under optimised fermentation conditions, apricot kernels hold promise as functional food ingredients due to the beneficial antioxidant properties observed in this study.
{"title":"Improving the Bioactivities of Apricot Kernels Through Fermentation: Investigating the Relationship Between Bioactivities, Polyphenols, and Amino Acids Through the Random Forest Regression XAI Approach.","authors":"Zhiyu Zhao, Kevin Kantono, Rothman Kam, Thao T Le, Eileen Kitundu, Tony Chen, Nazimah Hamid","doi":"10.3390/foods14050845","DOIUrl":"10.3390/foods14050845","url":null,"abstract":"<p><p>Apricot kernels are known for being a rich source of oil, protein, and bioactive compounds. This study focused on enhancing the bioactivities of apricot kernels through fermentation. Additionally, this study explored the correlations between polyphenols, amino acids, antioxidant activities, and total phenolic content (TPC). The findings indicated that apricot kernels fermented with <i>Lactiplantibacillus plantarum</i> exhibited increased antioxidant activity, as assessed by the FRAP and CUPRAC methods, and an increased TPC compared to naturally fermented samples. The CUPRAC activity increased significantly from 1.03 to 1.82 mg of ascorbic acid per gram of sample on day 7, and the FRAP activity increased from 4.9 to 12.2 mg of ascorbic acid per gram of sample on day 3 of fermentation. Moreover, the TPC significantly increased from 1.67 to 7.58 mg of gallic acid per gram of sample on day 9 of fermentation. The results further demonstrated that, during the fermentation process, the concentration of hydroxybenzoic acid increased from 0.52 µg/g on day 0 to 5.3 µg/g on day 9. The DL-3-phenyllactic acid content demonstrated a significant increase from 0.42 µg/g on day 0 to 99.62 µg/g on day 5, while the benzoic acid content exhibited a notable increase from 45.33 µg/g to 138.13 µg/g over the fermentation period, with peak levels being observed on day 5. Similarly, most amino acids demonstrated a rise in concentration as the fermentation progressed, peaking on the ninth day. This study further employed random forest regression as a form of explainable artificial intelligence (XAI) to explore the relationships between phenolic compounds, amino acids, and antioxidant activities. Amino acids like L-cystine and L-anserine were found to positively impact FRAP values, while L-histidine and 1-methyl-L-histidine contributed to the CUPRAC antioxidant activity. Notably, hydroxybenzoic acid emerged as a key contributor to both the FRAP value and TPC, highlighting its significance in improving the overall antioxidant capacity of apricot kernels. These findings indicate that, under optimised fermentation conditions, apricot kernels hold promise as functional food ingredients due to the beneficial antioxidant properties observed in this study.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Encapsulation techniques have emerged as an important strategy for creating nutraceuticals and fortified foods and beverages that contain various bioactive compounds, due to their potential health benefits [...].
{"title":"Encapsulation Processes: Valorization, Stabilization, and Commercialization of Active and Natural Compounds.","authors":"Berta Nogueiro Estevinho","doi":"10.3390/foods14050844","DOIUrl":"10.3390/foods14050844","url":null,"abstract":"<p><p>Encapsulation techniques have emerged as an important strategy for creating nutraceuticals and fortified foods and beverages that contain various bioactive compounds, due to their potential health benefits [...].</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the current scenario, food waste stands out as a pressing issue, accounting for a significant portion of the waste generated worldwide [...].
{"title":"Functional Ingredients from Food Waste and By-Products: Processing Technologies, Functional Characteristics and Value-Added Applications.","authors":"Lucía Seguí, Cristina Barrera","doi":"10.3390/foods14050847","DOIUrl":"10.3390/foods14050847","url":null,"abstract":"<p><p>In the current scenario, food waste stands out as a pressing issue, accounting for a significant portion of the waste generated worldwide [...].</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study evaluates the effects of fortifying bread with different concentrations (3%, 10%, and 30%) of Matricaria chamomilla L. (MC) infusion and powder, derived from the plant's aerial parts (stem, leaf, and flower). UPLC/MS-MS analysis of MC infusion and powder ethanolic extract confirmed the presence of polyphenolic compounds, including flavonoids, contributing to enhanced antioxidant and enzyme inhibitory properties. The physicochemical, antioxidant, and sensory properties of the enriched breads were assessed. Hierarchical cluster analysis revealed that breads enriched with 30% MC powder (BP-MC 30%) and infusion (BI-MC 30%) exhibited superior overall quality compared with other formulations. The enriched breads showed increased protein, fiber, and antioxidant content. Specifically, BI-MC 30% demonstrated superior antioxidant activity, while BP-MC 30% provided the highest fiber content. Sensory evaluation indicated that the enriched breads retained sensory properties similar to those of the control bread, despite the nutritional improvements. These findings suggest that incorporating Matricaria chamomilla, particularly at 30%, enhances the nutritional profile and antioxidant properties of bread while maintaining sensory characteristics close to those of traditional bread. This research highlights the potential of using chamomile's aerial parts in the valorization of plant-based by-products for functional bakery product development.
{"title":"Nutritional Composition, Physicochemical Properties, Antioxidant Activity, and Sensory Quality of <i>Matricaria chamomilla</i>-Enriched Wheat Bread.","authors":"Khawla Kerbab, Ibtissem Sanah, Fairouze Djeghim, Nadjah Belattar, Valentina Santoro, Maria D'Elia, Luca Rastrelli","doi":"10.3390/foods14050838","DOIUrl":"10.3390/foods14050838","url":null,"abstract":"<p><p>This study evaluates the effects of fortifying bread with different concentrations (3%, 10%, and 30%) of <i>Matricaria chamomilla</i> L. (MC) infusion and powder, derived from the plant's aerial parts (stem, leaf, and flower). UPLC/MS-MS analysis of MC infusion and powder ethanolic extract confirmed the presence of polyphenolic compounds, including flavonoids, contributing to enhanced antioxidant and enzyme inhibitory properties. The physicochemical, antioxidant, and sensory properties of the enriched breads were assessed. Hierarchical cluster analysis revealed that breads enriched with 30% MC powder (BP-MC 30%) and infusion (BI-MC 30%) exhibited superior overall quality compared with other formulations. The enriched breads showed increased protein, fiber, and antioxidant content. Specifically, BI-MC 30% demonstrated superior antioxidant activity, while BP-MC 30% provided the highest fiber content. Sensory evaluation indicated that the enriched breads retained sensory properties similar to those of the control bread, despite the nutritional improvements. These findings suggest that incorporating <i>Matricaria chamomilla</i>, particularly at 30%, enhances the nutritional profile and antioxidant properties of bread while maintaining sensory characteristics close to those of traditional bread. This research highlights the potential of using chamomile's aerial parts in the valorization of plant-based by-products for functional bakery product development.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aylín Araiza-Alvarado, Saúl Alberto Álvarez, José Alberto Gallegos-Infante, Jorge Alberto Sánchez-Burgos, Nuria Elizabeth Rocha-Guzmán, Silvia Marina González-Herrera, Martha Rocío Moreno-Jiménez, Rubén Francisco González-Laredo, Verónica Cervantes-Cardoza
Oak infusions enriched with agavins may offer nutraceutical benefits in the development of iced teas. This study evaluated infusions of Quercus sideroxyla and Quercus eduardii leaves formulated with different concentrations of agavins (0, 2, 6, and 10%), analyzing their physicochemical and sensory properties, chemical stability, and antioxidant capacity. The incorporation of agavins resulted in substantial modifications to physicochemical parameters, including pH, titratable acidity, and soluble solids, thereby enhancing product stability and consistency. Notable distinctions were observed between the two species with respect to their acidogenic response and soluble solid concentration. Interactions between agavins and phenolic compounds, as discerned by UPLC-PDA-ESI-MS/MS and FT-IR, exerted a significant influence on bioactivity of the phenolic constituents, thereby affecting the nutraceutical potential of the infusions. These interactions, facilitated by hydrogen bonds, led to reduction in phenolic acids, such as quinic acid (↓ 43%), and alteration in antioxidant capacity at high concentrations of agavins. The findings underscore the significance of meticulously designing balanced formulations that optimize chemical stability, functionality, and sensory acceptance, thereby ensuring the quality of the final product.
{"title":"Sweetening with Agavins: Its Impact on Sensory Acceptability, Physicochemical Properties, Phenolic Composition and Nutraceutical Potential of Oak Iced Tea.","authors":"Aylín Araiza-Alvarado, Saúl Alberto Álvarez, José Alberto Gallegos-Infante, Jorge Alberto Sánchez-Burgos, Nuria Elizabeth Rocha-Guzmán, Silvia Marina González-Herrera, Martha Rocío Moreno-Jiménez, Rubén Francisco González-Laredo, Verónica Cervantes-Cardoza","doi":"10.3390/foods14050833","DOIUrl":"10.3390/foods14050833","url":null,"abstract":"<p><p>Oak infusions enriched with agavins may offer nutraceutical benefits in the development of iced teas. This study evaluated infusions of <i>Quercus sideroxyla</i> and <i>Quercus eduardii</i> leaves formulated with different concentrations of agavins (0, 2, 6, and 10%), analyzing their physicochemical and sensory properties, chemical stability, and antioxidant capacity. The incorporation of agavins resulted in substantial modifications to physicochemical parameters, including pH, titratable acidity, and soluble solids, thereby enhancing product stability and consistency. Notable distinctions were observed between the two species with respect to their acidogenic response and soluble solid concentration. Interactions between agavins and phenolic compounds, as discerned by UPLC-PDA-ESI-MS/MS and FT-IR, exerted a significant influence on bioactivity of the phenolic constituents, thereby affecting the nutraceutical potential of the infusions. These interactions, facilitated by hydrogen bonds, led to reduction in phenolic acids, such as quinic acid (↓ 43%), and alteration in antioxidant capacity at high concentrations of agavins. The findings underscore the significance of meticulously designing balanced formulations that optimize chemical stability, functionality, and sensory acceptance, thereby ensuring the quality of the final product.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The shaping process is recognized as a crucial step in the manufacturing of green tea. However, its influence on aroma quality remains unclear. In this study, the effects of four shaping techniques, including flat green tea (FGT), straight green tea (SGT), phoenix green tea (PGT), and curled green tea (CGT), on the aroma quality and volatile metabolites of green tea were investigated by gas chromatography electronic nose (GC-E-Nose) and gas chromatography-tandem mass spectrometry (GC-MS/MS). The findings indicated that distinct shaping processes significantly influenced the development of the aroma quality and aroma components of green tea. The PGT processing facilitated the attainment of superior aroma quality of green tea. In total, 60 volatile components were identified by GC-MS/MS, with 54 of these compounds being consistently detected across four different shaping techniques. In particular, the PGT processing method was effective in yielding elevated levels of alcohols, esters and ketones. Moreover, 20 key odorants were screened out, with (E,E)-2,4-decadienal, (E,E)-2,4-nonadienal, phenylethyl alcohol, and benzeneacetaldehyde proven to be substantial contributors to the overall aromas of green tea under diverse shaping procedures. These key odorants were primarily derived from lipid degradation and the Maillard reaction. GC-E-Nose served as a significant adjunct to sensory evaluation, enabling the swift differentiation of green tea samples that have undergone various shaping processes. These findings offer both theoretical and technical perspectives that may guide the creation of innovative green tea products distinguished by their unique shapes.
{"title":"Influence of Different Shaping Techniques on the Aroma Quality and Volatile Metabolites of Green Tea Revealed by Gas Chromatography Electronic Nose and Gas Chromatography-Tandem Mass Spectrometry.","authors":"Jiahao Tang, Jiajing Hu, Xianxiu Zhou, Qiwei Wang, Yongwen Jiang, Haibo Yuan, Yujie Wang, Yanqin Yang","doi":"10.3390/foods14050816","DOIUrl":"10.3390/foods14050816","url":null,"abstract":"<p><p>The shaping process is recognized as a crucial step in the manufacturing of green tea. However, its influence on aroma quality remains unclear. In this study, the effects of four shaping techniques, including flat green tea (FGT), straight green tea (SGT), phoenix green tea (PGT), and curled green tea (CGT), on the aroma quality and volatile metabolites of green tea were investigated by gas chromatography electronic nose (GC-E-Nose) and gas chromatography-tandem mass spectrometry (GC-MS/MS). The findings indicated that distinct shaping processes significantly influenced the development of the aroma quality and aroma components of green tea. The PGT processing facilitated the attainment of superior aroma quality of green tea. In total, 60 volatile components were identified by GC-MS/MS, with 54 of these compounds being consistently detected across four different shaping techniques. In particular, the PGT processing method was effective in yielding elevated levels of alcohols, esters and ketones. Moreover, 20 key odorants were screened out, with (<i>E</i>,<i>E</i>)-2,4-decadienal, (<i>E</i>,<i>E</i>)-2,4-nonadienal, phenylethyl alcohol, and benzeneacetaldehyde proven to be substantial contributors to the overall aromas of green tea under diverse shaping procedures. These key odorants were primarily derived from lipid degradation and the Maillard reaction. GC-E-Nose served as a significant adjunct to sensory evaluation, enabling the swift differentiation of green tea samples that have undergone various shaping processes. These findings offer both theoretical and technical perspectives that may guide the creation of innovative green tea products distinguished by their unique shapes.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Yan, Ni Zhao, Jiaying Liu, Shengmei Zhang, Yinjie Zhang, Xuemei Qin, Kefeng Zhai, Chenhui Du
Ziziphi Spinosae Semen (ZSS), a homology of medicine and a type of seed, has been widely used to improve sleep quality. The present study aimed to assess the effects of ZSS flavonoid (ZSSF) extracted and isolated from ZSS on gut microbiota and hypothalamus metabolomic profiles in a chronic restraint stress (CRS)-induced anxiety mouse model. ZSSF was prepared using microporous resin chromatography, and seven compounds were determined by UPLC-MS. ZSSF treatment dramatically reduced anxiety-like behaviors, exerted sedative-hypnotic effects, increased hippocampal 5-HT and 5-HTP, and enhanced intestinal barrier function through inhibiting colon ZO-1, Claudin-1, and Occludin expression and reducing TNF-α, IL-6, and IL-1β levels. Compared with the CRS group, the diversity of gut microbiota in ZSSF-group mice was increased, with an increase in Bacteroidetes and a decrease in Firmicutes, and it was accompanied by an increase in fecal SCFAs. Hypothalamus metabolomics and lipidomics were performed to achieve 25 differential metabolites and 44 lipids, respectively. Serum metabolomics showed a total of 13 metabolites associated with anxiety were remarkably regulated by ZSSF. Weighted correlation network analysis (WGCNA) showed that glycerophospholipids (GPs) as well as phenylalanine, tyrosine, and L-tryptophan in peripheral and central parts were significant metabolites, which contributed to the pharmacological action of ZSSF. The mRNA expression of TPH2 and DDC key enzymes associated with tryptophan metabolism were upregulated, and PLA2G12A, LACT, and PLA2G6 key enzymes associated with GP metabolism were downregulated in ZSSF compared with CRS. Briefly, ZSSF improved tryptophan and GP metabolism and regulated the gut microbiome. This study may lay a theoretical basis for potentially developing ZSSF as a natural functional food ingredient for the improvement of anxiety and sleep disorders.
{"title":"Ziziphi Spinosae Semen Flavonoid Ameliorates Hypothalamic Metabolism and Modulates Gut Microbiota in Chronic Restraint Stress-Induced Anxiety-like Behavior in Mice.","authors":"Yan Yan, Ni Zhao, Jiaying Liu, Shengmei Zhang, Yinjie Zhang, Xuemei Qin, Kefeng Zhai, Chenhui Du","doi":"10.3390/foods14050828","DOIUrl":"10.3390/foods14050828","url":null,"abstract":"<p><p>Ziziphi Spinosae Semen (ZSS), a homology of medicine and a type of seed, has been widely used to improve sleep quality. The present study aimed to assess the effects of ZSS flavonoid (ZSSF) extracted and isolated from ZSS on gut microbiota and hypothalamus metabolomic profiles in a chronic restraint stress (CRS)-induced anxiety mouse model. ZSSF was prepared using microporous resin chromatography, and seven compounds were determined by UPLC-MS. ZSSF treatment dramatically reduced anxiety-like behaviors, exerted sedative-hypnotic effects, increased hippocampal 5-HT and 5-HTP, and enhanced intestinal barrier function through inhibiting colon ZO-1, Claudin-1, and Occludin expression and reducing TNF-α, IL-6, and IL-1β levels. Compared with the CRS group, the diversity of gut microbiota in ZSSF-group mice was increased, with an increase in <i>Bacteroidetes</i> and a decrease in <i>Firmicutes</i>, and it was accompanied by an increase in fecal SCFAs. Hypothalamus metabolomics and lipidomics were performed to achieve 25 differential metabolites and 44 lipids, respectively. Serum metabolomics showed a total of 13 metabolites associated with anxiety were remarkably regulated by ZSSF. Weighted correlation network analysis (WGCNA) showed that glycerophospholipids (GPs) as well as phenylalanine, tyrosine, and L-tryptophan in peripheral and central parts were significant metabolites, which contributed to the pharmacological action of ZSSF. The mRNA expression of TPH2 and DDC key enzymes associated with tryptophan metabolism were upregulated, and PLA2G12A, LACT, and PLA2G6 key enzymes associated with GP metabolism were downregulated in ZSSF compared with CRS. Briefly, ZSSF improved tryptophan and GP metabolism and regulated the gut microbiome. This study may lay a theoretical basis for potentially developing ZSSF as a natural functional food ingredient for the improvement of anxiety and sleep disorders.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ciprofloxacin is metabolized from enrofloxacin for use in poultry to manage respiratory and gastrointestinal diseases, raising concerns due to its widespread tissue distribution and prolonged systemic persistence. This lateral flow immunoassay was designed to detect ciprofloxacin using an alternative IgY antibody binded with gold nanoparticles to detect ciprofloxacin residue in raw pork meat samples. The developed strip test achieved adequate sensitivity and specificity under the optimized conditions for pH, which is 7.8, and 20% of MeOH in 0.01 M phosphate buffer containing 1% Tween-20 was used for the buffer composition. An antibody concentration of 1.25 µg/mL was used to bind with gold nanoparticles as a probe for detection. The concentration of the test line (coating antigen) and control line (anti-IgY secondary antibody) was 0.5 mg/mL and 0.2 mg/mL, respectively. The efficiency of the developed strip test showed sensitivity with a 50% inhibitory concentration (IC50) of ciprofloxacin at 7.36 µg/mL, and the limit of detection was 0.2 µg/mL. The proposed strategy exhibited potential for monitoring ciprofloxacin in raw pork samples.
{"title":"Immunoglobulin Y-Based Lateral Flow Immunoassay Strip Test for Detecting Ciprofloxacin Antibiotic in Raw Pork Samples.","authors":"Sumed Yadoung, Huan-Yuan Xu, Sirikwan Dokuta, Peerapong Jeeno, Pichamon Yana, Marninphan Thongkham, Korawan Sringarm, Ryoichi Ishimatsu, Zhen-Lin Xu, Surat Hongsibsong","doi":"10.3390/foods14050818","DOIUrl":"10.3390/foods14050818","url":null,"abstract":"<p><p>Ciprofloxacin is metabolized from enrofloxacin for use in poultry to manage respiratory and gastrointestinal diseases, raising concerns due to its widespread tissue distribution and prolonged systemic persistence. This lateral flow immunoassay was designed to detect ciprofloxacin using an alternative IgY antibody binded with gold nanoparticles to detect ciprofloxacin residue in raw pork meat samples. The developed strip test achieved adequate sensitivity and specificity under the optimized conditions for pH, which is 7.8, and 20% of MeOH in 0.01 M phosphate buffer containing 1% Tween-20 was used for the buffer composition. An antibody concentration of 1.25 µg/mL was used to bind with gold nanoparticles as a probe for detection. The concentration of the test line (coating antigen) and control line (anti-IgY secondary antibody) was 0.5 mg/mL and 0.2 mg/mL, respectively. The efficiency of the developed strip test showed sensitivity with a 50% inhibitory concentration (IC<sub>50</sub>) of ciprofloxacin at 7.36 µg/mL, and the limit of detection was 0.2 µg/mL. The proposed strategy exhibited potential for monitoring ciprofloxacin in raw pork samples.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11899701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kui Zhong, Ting Wen, Bolin Shi, Houyin Wang, Zhicong An, Lei Zhao, Hongliang Li
Emotions evoked by food are an important factor that strongly influences the predictive power of consumer acceptance and consumption behavior when choosing food. The emotion questionnaire is an efficient measurement tool that describes the emotions evoked by different foods on the market. However, there is rarely a list of emotion terms for specific foods, such as the pure dairy products on the market. The aim of this study was to develop an emotion scale to measure emotion responses for pure dairy products and to investigate its application in consumer testing. A total of 1340 Chinese subjects (67% female, 18~45 years old, consumers of pure milk) participated in the study. The study began with the screening and identifying of emotion terms for pure dairy products and developed a proposed pure dairy product Emotion Scale (P-PDPES) with 50 emotion terms. Subsequently, the structure and emotion terms of the P-PDPES were determined using EFA and a final pure dairy product Emotion Scale (PDPES) with 33 emotion terms was obtained. The results showed that the PDPES has good validity and reliability. Moreover, the PDPES was used in a consumer test to describe the emotions towards the commercial pure dairy products. This PDPES discriminated well the emotions evoked by different pure dairy products and also indicated a good relationship between the model factors and the liking scores of milk products. To summarize, PDPES is a suitable measurement tool for assessing the emotions to pure dairy products.
{"title":"An Exploratory Study on the Development of a Pure Dairy Product Emotion Scale (PDPES): A Study of Milk Consumers in China.","authors":"Kui Zhong, Ting Wen, Bolin Shi, Houyin Wang, Zhicong An, Lei Zhao, Hongliang Li","doi":"10.3390/foods14050827","DOIUrl":"10.3390/foods14050827","url":null,"abstract":"<p><p>Emotions evoked by food are an important factor that strongly influences the predictive power of consumer acceptance and consumption behavior when choosing food. The emotion questionnaire is an efficient measurement tool that describes the emotions evoked by different foods on the market. However, there is rarely a list of emotion terms for specific foods, such as the pure dairy products on the market. The aim of this study was to develop an emotion scale to measure emotion responses for pure dairy products and to investigate its application in consumer testing. A total of 1340 Chinese subjects (67% female, 18~45 years old, consumers of pure milk) participated in the study. The study began with the screening and identifying of emotion terms for pure dairy products and developed a proposed pure dairy product Emotion Scale (P-PDPES) with 50 emotion terms. Subsequently, the structure and emotion terms of the P-PDPES were determined using EFA and a final pure dairy product Emotion Scale (PDPES) with 33 emotion terms was obtained. The results showed that the PDPES has good validity and reliability. Moreover, the PDPES was used in a consumer test to describe the emotions towards the commercial pure dairy products. This PDPES discriminated well the emotions evoked by different pure dairy products and also indicated a good relationship between the model factors and the liking scores of milk products. To summarize, PDPES is a suitable measurement tool for assessing the emotions to pure dairy products.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jasmina Mitrevski, Nebojša Đ Pantelić, Jovanka Laličić-Petronijević, Jovana S Kojić, Snežana Zlatanović, Stanislava Gorjanović, Stevan Avramov, Margarita S Dodevska, Vesna V Antić
This study aimed to evaluate the potential of beetroot powder (BP) as a functional ingredient in biscuits by investigating its effects on nutritional composition, sensory properties, and glycemic response. The primary goal was to determine whether BP could serve as a natural alternative to synthetic additives while maintaining product stability and consumer acceptability. Biscuits were formulated by replacing spelt flour with 15, 20, and 25% BP. The functional impact of the BP was assessed based on betaine content, macro- and microelements, glycemic index (GI), and acrylamide concentration. Thermal analysis (DSC and TGA) and water activity measurements confirmed the BP's stability during six months of storage. Increased BP content led to higher betaine levels and mineral enrichment, particularly with potassium and phosphorus among the macroelements and zinc among the microelements. Sensory analysis identified biscuits with 20% BP as the most preferred, maintaining acceptable ratings even after six months. Hardness initially increased with BP incorporation but decreased over time (p < 0.05). The acrylamide content in the BP-enriched biscuits was significantly lower than in control samples and well below the reference safety threshold. Notably, consuming beetroot biscuits did not trigger a sharp postprandial glucose spike, with the GI of the most acceptable sample (20% BP) measured at 49 ± 11. These findings confirm that BP improves the nutritional and sensory characteristics of biscuits while ensuring product safety and stability, supporting its application as a natural functional ingredient in confectionery products.
{"title":"Low Glycemic Index Biscuits Enriched with Beetroot Powder as a Source of Betaine and Mineral Nutrients.","authors":"Jasmina Mitrevski, Nebojša Đ Pantelić, Jovanka Laličić-Petronijević, Jovana S Kojić, Snežana Zlatanović, Stanislava Gorjanović, Stevan Avramov, Margarita S Dodevska, Vesna V Antić","doi":"10.3390/foods14050814","DOIUrl":"10.3390/foods14050814","url":null,"abstract":"<p><p>This study aimed to evaluate the potential of beetroot powder (BP) as a functional ingredient in biscuits by investigating its effects on nutritional composition, sensory properties, and glycemic response. The primary goal was to determine whether BP could serve as a natural alternative to synthetic additives while maintaining product stability and consumer acceptability. Biscuits were formulated by replacing spelt flour with 15, 20, and 25% BP. The functional impact of the BP was assessed based on betaine content, macro- and microelements, glycemic index (GI), and acrylamide concentration. Thermal analysis (DSC and TGA) and water activity measurements confirmed the BP's stability during six months of storage. Increased BP content led to higher betaine levels and mineral enrichment, particularly with potassium and phosphorus among the macroelements and zinc among the microelements. Sensory analysis identified biscuits with 20% BP as the most preferred, maintaining acceptable ratings even after six months. Hardness initially increased with BP incorporation but decreased over time (<i>p</i> < 0.05). The acrylamide content in the BP-enriched biscuits was significantly lower than in control samples and well below the reference safety threshold. Notably, consuming beetroot biscuits did not trigger a sharp postprandial glucose spike, with the GI of the most acceptable sample (20% BP) measured at 49 ± 11. These findings confirm that BP improves the nutritional and sensory characteristics of biscuits while ensuring product safety and stability, supporting its application as a natural functional ingredient in confectionery products.</p>","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 5","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}