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High sensitivity intelligent packaging films harnessing rose anthocyanins and hydrophilic silica aerogel for visually food freshness monitoring 高灵敏度智能包装薄膜利用玫瑰花青素和亲水二氧化硅气凝胶视觉食品新鲜度监测
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-11 DOI: 10.1093/fqsafe/fyad051
Wenjun Wu, Luman Zheng, Jiahao Yu, Liming Liu, Gulden Goksen, Ping Shao
Abstract The enhancement of the sensitivity for anthocyanin-based indicator films in food freshness monitoring in real time is important for application. In this study, hydrophilic silica aerogel (SiO2NA) was incorporated into corn starch (CS)/chitosan (CH)/rose anthocyanins (RACNs)-encapsulated potato amylopectin nanoparticles (APNPs) composite film to increase the sensitivity for shrimp freshness detection. Microstructure of films revealed that the gas absorption capacity was improved by amorphous SiO2NA via hydrogen interactions. The pore size (1.74-5.60 times), pore volume (3.92-5.60 times), and specific surface area (2.21-2.34 times) of films increased with the addition of SiO2NA. The sensing to NH3 and pH, and reversibility of films were also reinforced. Meanwhile, the pH-responsible films containing SiO2NA changed visibly in color from purple-red to orange-gray and finally to grey, enabling an effective monitoring of shrimp freshness during storage at 4℃. Thus, anthocyanin-based indicator films with improved sensitivity by adding SiO2NA were successfully designed for monitoring shrimp freshness.
摘要提高花青素基指示膜在食品新鲜度实时监测中的灵敏度具有重要的应用价值。本研究将亲水性二氧化硅气凝胶(SiO2NA)掺入玉米淀粉(CS)/壳聚糖(CH)/玫瑰花青素(RACNs)包封的马铃薯支链淀粉纳米颗粒(APNPs)复合膜中,以提高对虾新鲜度检测的灵敏度。薄膜的微观结构表明,非晶SiO2NA通过氢相互作用提高了薄膜的气体吸收能力。随着SiO2NA的加入,膜的孔径(1.74 ~ 5.60倍)、孔体积(3.92 ~ 5.60倍)、比表面积(2.21 ~ 2.34倍)均有所增加。膜对NH3和pH的敏感性和可逆性也得到增强。同时,含SiO2NA的ph负责膜的颜色从紫红色到橙灰色,最后变为灰色,可以有效地监测虾在4℃储存期间的新鲜度。因此,通过添加SiO2NA,成功地设计了灵敏度提高的花青素指示膜,用于监测虾的新鲜度。
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引用次数: 0
Fate of ochratoxin A in dried red chilies during roasting process 干红辣椒在烘烤过程中赭曲霉毒素A的归宿
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-11 DOI: 10.1093/fqsafe/fyad054
Xiao Xiao, Jiang Chen, Qiujin Zhu, Jing Wan
Abstract Dried red chilies are widely used spices in the world and it is susceptible to contamination by fungi and fungal toxins. Roasting is a common way of processing dried red chilies. This study explored the effects of Aspergillus niger and ochratoxin A (OTA) contamination on the quality of roasted chilies, and the fate of OTA during the roasting process. Three optimum roasting conditions (140°C × 8 min, 160°C × 6 min and 180°C × 4 min) were screened out by a combination of instrumental and manual sensory evaluations. Under these roasting conditions A. niger and OTA contamination diminished the quality and tastes of roasted chilies. With the increase of roasting temperature and time duration, OTA contents and mold counts gradually decreased, together with the DNA degradation of OTA biosynthesis related genes of A. niger in roasted chilies. The roasting condition at 180°C × 4 min showed the great decomposition effect on OTA, while also maintaining the good sensory quality of roasted chilies. This study shed light on the fate of OTA and during chili roasting process.
摘要干辣椒是世界上广泛使用的香料,它容易受到真菌和真菌毒素的污染。烤是处理干红辣椒的一种常用方法。本研究探讨了黑曲霉和赭曲霉毒素A (OTA)污染对烤辣椒品质的影响,以及OTA在烤辣椒过程中的命运。通过仪器和人工感官综合评价筛选出3种最佳焙烧条件(140°C × 8 min、160°C × 6 min和180°C × 4 min)。在这些烘烤条件下,黑曲霉和OTA污染降低了烤辣椒的质量和味道。随着烘烤温度和烘烤时间的增加,烤辣椒中OTA含量和霉菌数量逐渐减少,同时黑黑霉OTA生物合成相关基因DNA降解。180℃× 4 min的焙烧条件对OTA有较大的分解作用,同时保持了焙烧辣椒的良好感官品质。这项研究揭示了OTA的命运和辣椒的烘烤过程。
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引用次数: 0
Genome-wide identification of the histone acetyltransferase gene family in Citrus clementina and its potential roles in citrate metabolism 柑橘组蛋白乙酰转移酶基因家族的全基因组鉴定及其在柠檬酸盐代谢中的潜在作用
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-11-11 DOI: 10.1093/fqsafe/fyad052
Xia-hui Lin, Yan-na Shi, Sheng-chao Liu, Xin-cheng Liu, Ming-lei Zhang, Bo Zhang, Shao-jia Li, Kun-song Chen
Abstract Histone acetyltransferase (HAT) catalyzes histone acetylation and is involved in plant growth and development and stress responses. Here, 37 CitHAT genes were identified in Citrus clementina. Their physicochemical properties, chromosomal location, gene structure, conserved domain and motif, and cis-acting elements were characterized. CitHATs were classified into four subfamilies based on protein sequence homology, which was strongly supported by gene structure, conserved domain, and motif analysis. The cis-acting elements in gene promoter regions were predicted to be associated with the regulation of plant growth, stress resistance, and response to hormones. Phenotypic and transcriptomic analyses of citrus callus with the mock treatment and HAT inhibitor treatment revealed that increased citric acid content in inhibitor treatment may be attributed to differential expression of CitPEPCK2 and CitGS2, which may be due to the hypo-acetylation of histone H3. The expression patterns of CitHATs in citrus fruit development stages showed that CitHAG11 and CitHAG28 exhibited a negative correlation with citric acid content. Our study associates the potential function of histone acetyltransferases in citrate metabolism and extends the molecular mechanism of citrate metabolism in fruits.
组蛋白乙酰转移酶(Histone acetyltransferase, HAT)是一种催化组蛋白乙酰化的酶,参与植物的生长发育和逆境反应。本研究共鉴定了柑橘中37个CitHAT基因。鉴定了它们的理化性质、染色体定位、基因结构、保守结构域和基序以及顺式作用元件。基于基因结构、保守结构域和基序分析,将其划分为4个亚家族。基因启动子区域的顺式作用元件被认为与植物生长、抗逆性和激素应答的调控有关。模拟处理和HAT抑制剂处理的柑橘愈伤组织表型和转录组学分析表明,抑制剂处理的柑橘愈伤组织中柠檬酸含量的增加可能是由于组蛋白H3的低乙酰化导致的CitPEPCK2和CitGS2的表达差异。柑橘果实发育阶段CitHAG11和CitHAG28的表达与柠檬酸含量呈负相关。本研究揭示了组蛋白乙酰转移酶在枸橼酸代谢中的潜在功能,并拓展了果实中枸橼酸代谢的分子机制。
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引用次数: 0
Titanium dioxide E171 consumption exacerbates Listeria monocytogenes infection in mice 二氧化钛E171的摄入加剧了小鼠单核细胞增生李斯特菌的感染
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-27 DOI: 10.1093/fqsafe/fyad048
Yue Teng, Ailin Wang, Dongyun Zhao, Guopeng Li, Longze Liu, Yue Zou, Xiaodong Xia
Abstract The food additive titanium dioxide is commonly utilized to enhance the appearance and flavor of food products. However, this substance has been linked to gastrointestinal disorders. The aim of this study was to investigate the impact of dietary exposure of titanium dioxide E171 on Listeria monocytogenes infection in mice. Mice were mainly divided to control, LM group (L. monocytogenes infection) and E171+LM group (E171 supplementation before L. monocytogenes infection). Pre-exposure to E171 resulted in increased bacterial counts in the liver, spleen, ileum, colon, mesenteric lymph nodes and feces of mice after L. monocytogenes infection. Moreover, E171 exposure increased the levels pro-inflammatory cytokines while attenuating the levels of anti-inflammatory cytokines in mice infected with L. monocytogenes. Meanwhile, mice in E171+LM group exhibited considerably more severe colonic inflammation and worse intestinal barrier function than mice in LM group. The 16S rRNA gene sequencing revealed a shift in the composition of the gut microbiota of mice in E171+LM group, characterized by a decrease in the relative abundance of Firmicutes and a decrease in the Firmicutes-to-Bacteroidetes ratio. The levels of acetate, butyrate, and isobutyrate were markedly decreased within the cecum of mice in E171+LM group in comparison to mice in LM group. In conclusion, these results suggest that E171 exposure could exacerbate L. monocytogenes infection in mice, which may provide useful information for future risk assessment of this commonly used food additive.
摘要二氧化钛是一种常用的食品添加剂,用于提高食品的外观和风味。然而,这种物质与胃肠道疾病有关。本研究旨在探讨饮食中二氧化钛E171对小鼠单核细胞增生李斯特菌感染的影响。小鼠主要分为对照组、LM组(单核增生乳杆菌感染)和E171+LM组(单核增生乳杆菌感染前补充E171)。预暴露E171导致小鼠感染单核细胞增生乳杆菌后肝脏、脾脏、回肠、结肠、肠系膜淋巴结和粪便中的细菌数量增加。此外,E171暴露增加了单核增生乳杆菌感染小鼠的促炎细胞因子水平,同时降低了抗炎细胞因子水平。同时,E171+LM组小鼠的结肠炎症程度明显高于LM组,肠道屏障功能明显低于LM组。16S rRNA基因测序显示,E171+LM组小鼠肠道菌群的组成发生了变化,其特征是厚壁菌门(Firmicutes)的相对丰度降低,厚壁菌门(Firmicutes)与拟杆菌门(bacteroidetes)的比例降低。与LM组相比,E171+LM组小鼠盲肠内乙酸、丁酸和异丁酸水平明显降低。综上所述,E171暴露可加重小鼠单核细胞增生性乳杆菌感染,为今后该常用食品添加剂的风险评估提供参考。
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引用次数: 0
Can natural preservatives serve as a new line of protective technology against bacterial pathogens in meat and meat products? 天然防腐剂能否作为一种新的保护技术,防止肉类和肉制品中的细菌病原体?
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-19 DOI: 10.1093/fqsafe/fyad049
Changyong Cheng, Lingli Jiang, Xiaoliang Li, Houhui Song, Weihuan Fang
Abstract Contamination of meats and meat products by pathogenic microorganisms is responsible for significant percentage of outbreaks of foodborne illness. There are also concerns over the carcinogenic potential of dietary nitrate and nitrite in the processed meat products. The past few decades have seen extensive search for novel technologies alternative to synthetic chemical preservatives to reduce the level of contamination of foods by pathogenic and spoilage microbes. This review provides general overview of natural preservatives with potential applications in the meat industry, including phages and their endolysins, bacteriocins, microbial lipopeptides, antimicrobial peptides of plant or insect origin, and essential oils or extracts of plant origins. Instead of providing summary data from the published literature, we attempted to elaborate the challenges facing the development of novel natural preservatives as the antimicrobial hurdles, taking into consideration of the fact of sharp contrast between extensive studies in this particular field and very limited industrial use. More specifically, we emphasized the great importance of having streamlined approaches and methodological guidelines in research and development of natural preservatives so that the journey to their industrial use for safer meats and meat products could be shortened or made easier.
致病性微生物对肉类和肉制品的污染是食源性疾病爆发的重要原因。还有人担心加工肉制品中的膳食硝酸盐和亚硝酸盐可能致癌。在过去的几十年里,人们一直在广泛寻找替代合成化学防腐剂的新技术,以降低致病性和腐败性微生物对食品的污染水平。本文综述了具有潜在应用前景的天然防腐剂,包括噬菌体及其内溶素、细菌素、微生物脂肽、植物或昆虫来源的抗菌肽以及植物来源的精油或提取物。考虑到在这一特定领域的广泛研究与非常有限的工业应用之间的鲜明对比,我们没有从已发表的文献中提供总结数据,而是试图详细阐述新型天然防腐剂作为抗菌障碍所面临的挑战。更具体地说,我们强调了在天然防腐剂的研究和开发中采用简化的方法和方法指南的重要性,以便缩短或简化将天然防腐剂用于更安全的肉类和肉类产品的工业应用的过程。
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引用次数: 0
Transcription factors involved in tomato fruit quality improvement 番茄果实品质改良的转录因子研究
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-19 DOI: 10.1093/fqsafe/fyad050
Yanna Shi, Zhengrong Dai, Kunsong Chen
Abstract Fruit quality is both a consumer demand and an important goal for horticultural breeding. The exploration of quality-related genes provides targets to create high-quality germplasm using biotechnology, of which transcription factors (TFs) exhibit dosage effects compared with structural genes. Tomato, an important horticultural crop, as well as model fruit has been widely studied. Here, we focused on TFs with potential utilization value that control appearance (e.g., shape, pigment) and internal (e.g., flavor, texture, shelf life and health benefits) quality in tomato. Additionally, potential strategies to generate high-quality tomato fruit are discussed.
摘要果实品质既是消费者的需求,也是园艺育种的重要目标。质量相关基因的探索为利用生物技术创造优质种质提供了靶点,其中转录因子与结构基因相比表现出剂量效应。番茄是一种重要的园艺作物,也是一种模式水果,受到了广泛的研究。在此,我们重点研究了具有潜在利用价值的控制番茄外观(如形状、色素)和内部(如风味、质地、保质期和健康益处)质量的TFs。此外,还讨论了生产高品质番茄果实的潜在策略。
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引用次数: 0
Effects of thidiazuron on the quality and storage properties of mango fruit during postharvest 噻唑脲对芒果采后果实品质及贮藏特性的影响
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-14 DOI: 10.1093/fqsafe/fyad047
Tangxin Jiang, Cong Luo, Wen Wan, Rongzhen Liang, Tingting Lu, Yuze Li, Fangfang Xie, Canbin Chen, Xi Li, Xiaona Xie, Xinhua He
Abstract Thidiazuron (TDZ) is used for the expansion of fruits, but excessive levels of TDZ lead to a decline in fruit quality. However, the appropriate concentration of TDZ for mango expansion without a decline in quality is not clear. In the present study, four different concentrations (5 mg/L, 10 mg/L, 15 mg/L and 20 mg/L) of TDZ were applied via spraying on mango plants, and several physiological and biochemical indicators were measured. The results showed that TDZ treatment significantly increased mango fruit size and single-fruit weight. In mango fruit, TDZ treatment decreased the disease index, delayed the increase in the malondialdehyde (MDA) and H2O2 content, and maintained firmness and antioxidant capacities at a relatively high level during postharvest storage. At the same time, TDZ treatment delayed the decrease in the GA3, IAA and JA contents in mango and reduced the accumulation of ABA and ethylene. These trends are consistent with TDZ treatment leading to extension of the shelf life of mango. Furthermore, ethylene biosynthesis, signal transduction and cell wall dismantling-related genes were investigated. The results indicated that the expression of the MiACS, MiETR2, MiERF113, MiERF010, MiERF054, MiEXP, MiPG14, MiPG21, MiCEL and MiPEL genes in mango was inhibited under TDZ treatment compared with the control. In summary, TDZ treatment can significantly increase the size and weight of mango fruit and can extend its shelf life. The most suitable concentration is 10~15 mg/L TDZ, which will not affect the quality of mango fruit.
摘要噻唑脲(Thidiazuron, TDZ)用于扩大果实,但过量的TDZ会导致果实品质下降。然而,TDZ的适宜浓度是否能使芒果膨化而不降低其品质尚不明确。本研究采用5 mg/L、10 mg/L、15 mg/L和20 mg/L 4种不同浓度的TDZ喷施于芒果植株,测定了几种生理生化指标。结果表明,TDZ处理显著提高了芒果果实大小和单果重。在芒果果实中,TDZ处理降低了病害指数,延缓了丙二醛(MDA)和H2O2含量的增加,并在采后贮藏期间保持了较高的硬度和抗氧化能力。同时,TDZ处理延缓了芒果中GA3、IAA和JA含量的下降,减少了ABA和乙烯的积累。这些趋势与TDZ处理导致芒果保质期延长是一致的。此外,还研究了乙烯生物合成、信号转导和细胞壁拆除相关基因。结果表明,与对照相比,TDZ处理抑制了芒果中MiACS、miertr2、MiERF113、MiERF010、MiERF054、MiEXP、MiPG14、MiPG21、MiCEL和MiPEL基因的表达。综上所述,TDZ处理可以显著增加芒果果实的大小和重量,延长其货架期。TDZ最适宜的浓度为10~15 mg/L,不会影响芒果果实的品质。
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引用次数: 0
Effect of gum tragacanth-sodium alginate coatings incorporated with epigallocatechin gallate on the quality and shelf life of large yellow croaker (Larimichthys crocea) during superchilling storage 表没食子儿茶素没食子酸酯包覆黄花胶-海藻酸钠对大黄鱼超低温贮藏质量和保质期的影响
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-14 DOI: 10.1093/fqsafe/fyad039
Zhilong Xu, Juxin Pei, Jun Mei, Huijie Yu, Siyuan Chu, Jing Xie
Abstract The effect of gum tragacanth (GT) and sodium alginate (SA) active coatings incorporated with epigallocatechin gallate (EGCG, 0.16%, 0.32% and 0.64%, respectively) on the quality of large yellow croaker fillets was evaluated during superchilling storage at -3 ℃ for 42 days. The results revealed that the GT-SA-EGCG active coatings delayed the microbial spoilage via reducing the numbers of total viable counts, Pseudomonas spp. and psychrophiles bacteria, retarded degradation of the physical properties of water holding capacity, cooking loss, color, odor and texture. GT-SA-EGCG coatings could also maintain the fillets’ freshness by keeping lower total volatile basic nitrogen, K value and histidine content, inhibiting myofibrillar protein degradation during superchilling storage. These results suggested that the active coatings embedded with GT-SA-EGCG could be used as a preservative to enhance the quality of large yellow croaker and prolong the shelf life by 7-14 days during superchilling storage at -3 ℃.
摘要在-3℃过冷保存42 d的条件下,研究了黄花胶(GT)和海藻酸钠(SA)活性涂层与表没食子儿茶素没食子酸酯(EGCG,分别为0.16%、0.32%和0.64%)对大黄鱼鱼片品质的影响。结果表明,GT-SA-EGCG活性膜通过减少总活菌数、假单胞菌和嗜冷细菌的数量,延缓了保水能力、蒸煮损失、颜色、气味和质地等物理性质的降解,延缓了微生物的腐败。GT-SA-EGCG包膜通过降低挥发性碱性氮、K值和组氨酸含量,抑制过冷贮藏过程中肌纤维蛋白的降解,保持鱼片的新鲜度。综上所述,以GT-SA-EGCG包埋的活性包膜可作为大黄鱼保鲜剂,在-3℃超冷条件下可提高大黄鱼品质,延长保鲜期7 ~ 14 d。
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引用次数: 0
Structure optimization of Pulsed Electric Field processor and its sterilization effect 脉冲电场处理机的结构优化及其杀菌效果
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-13 DOI: 10.1093/fqsafe/fyad044
Ye Tian, Wenshuo Fan, Weijian Lu, Guanjun Zhang, Zhengshi Chang
Abstract Pulsed electric field (PEF) technology is a highly promising nonthermal processing technique, which holds great potential for sterilizing liquid food. The effectiveness of this treatment depends on two crucial factors: pulse parameters and the structure of the processor. In this study, we focus on optimizing the shape of insulators within the PEF processor to improve the sterilization effect. This research provides a reliable technical reference for designing industrial equipment. The numerical simulation of multi-physics fields was employed to analyze the temperature, electric field, and fluid field distributions for different insulators. Ultimately, the geometric structure of the insulator was optimized by embedding a 0.7 mm arc. Sterilization experiments were then conducted on E. coli and S. aureus suspension with the conductivity similar to real grapefruit juice. The sterilization effect of the processor with the optimized structure and the maximum applied voltage was examined at the same driven frequency. The results indicate that compared to the original structure, there is an increase in withstanding voltage by 5-10 kV while maintaining significantly improved bactericidal effects at the same applied voltage. Furthermore, we preliminarily discussed the sterilization mechanism by combining electroporation theory with S. aureus’ electroporation threshold.
脉冲电场(PEF)技术是一种非常有前途的非热加工技术,在液态食品灭菌方面具有很大的潜力。这种处理的有效性取决于两个关键因素:脉冲参数和处理器的结构。在本研究中,我们着重于优化PEF处理器内绝缘子的形状,以提高灭菌效果。该研究为工业设备的设计提供了可靠的技术参考。采用多物理场数值模拟的方法,分析了不同绝缘子的温度、电场和流场分布。最后,通过嵌入0.7 mm电弧优化了绝缘子的几何结构。然后对电导率与真葡萄柚汁相似的大肠杆菌和金黄色葡萄球菌悬浮液进行灭菌实验。在相同驱动频率下,对结构优化后的处理器在最大外加电压下的杀菌效果进行了研究。结果表明,与原结构相比,在相同电压下,耐电压提高了5 ~ 10 kV,同时杀菌效果显著提高。此外,我们将电穿孔理论与金黄色葡萄球菌的电穿孔阈值相结合,初步探讨了其杀菌机理。
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引用次数: 0
Emerging tropane alkaloids: Global development and potential health threats 新兴的tropane生物碱:全球发展和潜在的健康威胁
3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-10-13 DOI: 10.1093/fqsafe/fyad043
Zile Wang, Shiqi Chen, Pimiao Zheng, Zhenhui Ren, Huixia Zhang, Jing Zhang, Bing Shao, Congming Wu, Haiyang Jiang
Abstract Tropane alkaloids (TAs) are a kind of plant secondary metabolites that mainly originate from Solanaceae. They have potent anticholinergic activity, and are well-known anticholinergic drugs, but also have been reported as plant toxins. Many studies have been conducted on TAs, but no scientometric research has been performed. This study aimed to expound the knowledge network and development of the field of TAs and predict the emerging development topics based on bibliometrics. In particular, this research combined VOSviewer and CiteSpace for visualization and covered 1,298 related scientific publications (1953–2022). The research on TAs is rapidly developing, with the participation of 86 countries, 1,129 research institutions, and 4,087 researchers. China, the University of Geneva, and Christen P. are the most productive country, institutions, and researchers, respectively. Currently, the main research topics on TAs include biosynthesis, in vitro synthesis, chemical synthesis, and determination methods. Since 2018, the monitoring of TAs in food related to human health has shown citation burst characteristics, which is considered an emerging development trend in the future. The findings of this study will enable researchers to quickly tap into the knowledge background/structure of TAs from massive data to provide a reference for further research.
Tropane生物碱(TAs)是一类主要来源于茄科植物的次生代谢产物。它们具有强效的抗胆碱能活性,是众所周知的抗胆碱能药物,但也有作为植物毒素的报道。关于ta的研究很多,但还没有进行科学计量学的研究。本研究旨在以文献计量学为基础,阐述科技资讯科技领域的知识网络与发展,并预测新兴的发展议题。特别地,本研究结合了VOSviewer和CiteSpace进行可视化,涵盖了1298篇相关的科学出版物(1953-2022)。TAs研究正在迅速发展,有86个国家、1129个研究机构、4087名研究人员参与其中。中国、日内瓦大学和Christen p分别是生产力最高的国家、机构和研究人员。目前,TAs的主要研究方向包括生物合成、体外合成、化学合成、测定方法等。2018年以来,与人类健康相关的食品中TAs的监测呈现引文爆发特征,被认为是未来的新兴发展趋势。本研究的发现将使研究人员能够从海量数据中快速挖掘ta的知识背景/结构,为进一步的研究提供参考。
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引用次数: 0
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Food Quality and Safety
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