IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-12 DOI: 10.1016/j.gene.2025.149954

Han Ouyang , Jiayi Peng , Zhouqi Huang , Yicong Huang , Yinglan Wen , Yuan Xu , Yu Long , Huijiao Lin , Qiyan Fu , Zhaojian Ding

Fusarium oxysporum f. sp. cubense (Foc), the causal agent of banana Fusarium wilt, is one of the most destructive plant pathogens worldwide. However, the molecular mechanisms that integrate fungal growth, metabolism, and virulence remain poorly understood. This study identifies and functionally characterizes FoSrk1, a serine/arginine-rich protein kinase (SRPK), in Foc. Deletion of FoSrk1 markedly impaired vegetative growth, reduced aerial mycelium formation and conidiation, and significantly attenuated virulence toward banana plantlets. The mutant also exhibited compromised utilization of diverse carbon sources, accompanied by altered expression of 32 genes involved in carbon metabolism. Complementation with the wild-type FoSrk1 allele restored normal growth and pathogenicity, confirming the gene’s essential role. Transcriptomic analyses revealed that loss of FoSrk1 altered the expression of 2,500 genes and was associated with changes in 37 alternative splicing events, predominantly intron retention, affecting transcripts linked to protein metabolism, ribosome biogenesis, and redox processes. Moreover, several virulence-associated genes encoding ABC transporters, cytochrome P450 enzymes, and hydrophobins were downregulated in the FoSrk1-deficient strain. These findings suggest that FoSrk1 acts as an important regulator that couples RNA processing with metabolic and pathogenic pathways and is required for normal fungal development and virulence. This study provides new insight into the molecular basis of pathogenic adaptation and identifies FoSrk1 as a potential target for antifungal intervention strategies.

香蕉枯萎病病原菌Fusarium oxysporum f. sp. cubense （Foc）是世界上最具破坏性的植物病原体之一。然而，整合真菌生长、代谢和毒力的分子机制仍然知之甚少。本研究鉴定并功能表征了FoSrk1，一种在Foc中富含丝氨酸/精氨酸的蛋白激酶（SRPK）。缺失FoSrk1显著损害香蕉植株的营养生长，减少气生菌丝的形成和分生，并显著降低对香蕉植株的毒力。该突变体还表现出对不同碳源的利用受损，并伴有涉及碳代谢的32个基因的表达改变。与野生型FoSrk1等位基因的互补恢复了正常的生长和致病性，证实了该基因的重要作用。转录组学分析显示，FoSrk1的缺失改变了2500个基因的表达，并与37个可变剪接事件的变化有关，主要是内含子保留，影响与蛋白质代谢、核糖体生物发生和氧化还原过程相关的转录本。此外，编码ABC转运蛋白、细胞色素P450酶和疏水蛋白的几个毒力相关基因在fosrk1缺陷菌株中下调。这些发现表明，FoSrk1是一个重要的调节因子，将RNA加工与代谢和致病途径结合起来，是正常真菌发育和毒力所必需的。该研究为致病适应的分子基础提供了新的见解，并确定了FoSrk1作为抗真菌干预策略的潜在靶点。

{"title":"SR protein kinase FoSrk1 integrates RNA splicing, carbon metabolism, and virulence in Fusarium oxysporum f. sp. cubense","authors":"Han Ouyang , Jiayi Peng , Zhouqi Huang , Yicong Huang , Yinglan Wen , Yuan Xu , Yu Long , Huijiao Lin , Qiyan Fu , Zhaojian Ding","doi":"10.1016/j.gene.2025.149954","DOIUrl":"10.1016/j.gene.2025.149954","url":null,"abstract":"<div><div><em>Fusarium oxysporum</em> f. sp. <em>cubense</em> (Foc), the causal agent of banana Fusarium wilt, is one of the most destructive plant pathogens worldwide. However, the molecular mechanisms that integrate fungal growth, metabolism, and virulence remain poorly understood. This study identifies and functionally characterizes FoSrk1, a serine/arginine-rich protein kinase (SRPK), in Foc. Deletion of <em>FoSrk1</em> markedly impaired vegetative growth, reduced aerial mycelium formation and conidiation, and significantly attenuated virulence toward banana plantlets. The mutant also exhibited compromised utilization of diverse carbon sources, accompanied by altered expression of 32 genes involved in carbon metabolism. Complementation with the wild-type <em>FoSrk1</em> allele restored normal growth and pathogenicity, confirming the gene’s essential role. Transcriptomic analyses revealed that loss of <em>FoSrk1</em> altered the expression of 2,500 genes and was associated with changes in 37 alternative splicing events, predominantly intron retention, affecting transcripts linked to protein metabolism, ribosome biogenesis, and redox processes. Moreover, several virulence-associated genes encoding ABC transporters, cytochrome P450 enzymes, and hydrophobins were downregulated in the <em>FoSrk1</em>-deficient strain. These findings suggest that FoSrk1 acts as an important regulator that couples RNA processing with metabolic and pathogenic pathways and is required for normal fungal development and virulence. This study provides new insight into the molecular basis of pathogenic adaptation and identifies FoSrk1 as a potential target for antifungal intervention strategies.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"983 ","pages":"Article 149954"},"PeriodicalIF":2.4,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two transcription factors and one antisense RNA underlie the thermoregulated insect pathogenicity of Yersinia entomophaga MH96 两个转录因子和一个反义RNA是虫食耶尔森菌MH96热调控昆虫致病性的基础。

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-11 DOI: 10.1016/j.gene.2025.149955

Amber R. Paulson , Maureen O’Callaghan , Xue-Xian Zhang , Naran Naren , Marion Schoof , Mark R.H. Hurst

Entomopathogenic bacteria express virulence genes in a temperature-dependent manner, but the underlying molecular mechanisms remain poorly understood. Here, we describe the roles of an unusual LytTR-containing transcription factor Yen6 in determining the virulence of Yersinia entomophaga (MH96). An initial transcriptome analysis in vivo revealed that yen6, located 1,372 bp upstream from genes encoding the Yen Toxin complex (Yen-Tc), exhibited significantly higher transcriptional activity at 37 °C compared to 25 °C (adjusted p-value < 0.001) during intrahemocoelic infection in the insect model Galleria‍ mellonella. Deletion of yen6 also significantly reduced MH96′s virulence in G.‍‍ mellonella by up to three-fold. Next, comparing the transcriptome of wild-type MH96 and its derived Δyen6 mutant revealed genes for ribose utilization were downregulated whereas genes for fructose utilization and the RNA-binding protein YhbY were upregulated in the yen6-deficient mutant. Subsequent electromobility shift assays showed that purified Yen6_His6 protein was capable of specifically binding to the promoter regions of the ribose and fructose utilization-related gene clusters, as well as yhbY. A 645-nucleotide-long 3′ untranslated region, designated yen7_AS, was identified extending from the yen6 coding region. Notably, yen7_AS completely overlaps the yen7 gene on the opposing strand and may therefore act as a cis anti-sense RNA regulating yen7 expression, which encodes a putative transcriptional regulator of the Yen-Tc. This study highlights the critical role of the yen6–yen7 locus in MH96 virulence during G.‍ mellonella infection, including the complex mechanisms controlling insecticidal exoprotein production.

昆虫致病细菌以温度依赖的方式表达毒力基因，但其潜在的分子机制仍然知之甚少。在这里，我们描述了一种不寻常的含有lyttr的转录因子Yen6在决定食虫耶尔森菌（MH96）毒力中的作用。初步的体内转录组分析显示，位于编码Yen毒素复合物（Yen- tc）基因上游1,372 bp处的yen6在37 °C时的转录活性明显高于25 °C（调整p值 ），His6蛋白能够特异性结合核糖和果糖利用相关基因簇的启动子区域，以及yhbY。从yen6编码区延伸出一个645个核苷酸长的3'非翻译区，命名为yen7_AS。值得注意的是，yen7_AS在相反的链上与yen7基因完全重叠，因此可能作为顺式反义RNA调节yen7的表达，从而编码推测的Yen-Tc的转录调节因子。该研究强调了yen6-yen7位点在G.‍mellonella感染期间MH96毒力中的关键作用，包括控制杀虫外蛋白产生的复杂机制。

{"title":"Two transcription factors and one antisense RNA underlie the thermoregulated insect pathogenicity of Yersinia entomophaga MH96","authors":"Amber R. Paulson , Maureen O’Callaghan , Xue-Xian Zhang , Naran Naren , Marion Schoof , Mark R.H. Hurst","doi":"10.1016/j.gene.2025.149955","DOIUrl":"10.1016/j.gene.2025.149955","url":null,"abstract":"<div><div>Entomopathogenic bacteria express virulence genes in a temperature-dependent manner, but the underlying molecular mechanisms remain poorly understood. Here, we describe the roles of an unusual LytTR-containing transcription factor Yen6 in determining the virulence of <em>Yersinia entomophaga</em> (MH96). An initial transcriptome analysis <em>in vivo</em> revealed that <em>yen6</em>, located 1,372 bp upstream from genes encoding the Yen Toxin complex (Yen-Tc), exhibited significantly higher transcriptional activity at 37 °C compared to 25 °C (adjusted <em>p</em>-value < 0.001) during intrahemocoelic infection in the insect model <em>Galleria‍ mellonella</em>. Deletion of <em>yen6</em> also significantly reduced MH96′s virulence in <em>G.‍‍ mellonella</em> by up to three-fold. Next, comparing the transcriptome of wild-type MH96 and its derived Δ<em>yen6</em> mutant revealed genes for ribose utilization were downregulated whereas genes for fructose utilization and the RNA-binding protein YhbY were upregulated in the <em>yen6-</em>deficient mutant. Subsequent electromobility shift assays showed that purified Yen6<sub>His6</sub> protein was capable of specifically binding to the promoter regions of the ribose and fructose utilization-related gene clusters, as well as <em>yhbY</em>. A 645-nucleotide-long 3′ untranslated region, designated <em>yen7_AS</em>, was identified extending from the <em>yen6</em> coding region. Notably, <em>yen7_AS</em> completely overlaps the <em>yen7</em> gene on the opposing strand and may therefore act as a <em>cis</em> anti-sense RNA regulating <em>yen7</em> expression, which encodes a putative transcriptional regulator of the Yen-Tc. This study highlights the critical role of the <em>yen6–yen7</em> locus in MH96 virulence during <em>G.‍ mellonella</em> infection<em>,</em> including the complex mechanisms controlling insecticidal exoprotein production.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"980 ","pages":"Article 149955"},"PeriodicalIF":2.4,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145752086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association study of tolerance to acute hypoxia in the olive flounder (Paralichthys olivaceus) using individual blood cortisol levels as a physiological phenotype 橄榄比目鱼对急性缺氧耐受性的全基因组关联研究，使用个体血液皮质醇水平作为生理表型

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-11 DOI: 10.1016/j.gene.2025.149952

M.A.H. Dilshan , Gaeun Kim , W.K.M. Omeka , D.S. Liyanage , H.M.V. Udayantha , D.C.G. Rodrigo , G.A.N.P. Ganepola , H.A.C.R. Hanchapola , Y.K. Kodagoda , Po Gong , Jihun Lee , Sukkyoung Lee , Jeongeun Kim , Jehee Lee

Hypoxia, caused by reduced dissolved oxygen (DO) levels in water, is a critical challenge in aquaculture and often results in stress, anorexia, and mass mortality in fish. The olive flounder (Paralichthys olivaceus) is a key species in South Korea’s global aquaculture exports. However, aquaculture production of P. olivaceus experiences significant economic losses caused by hypoxia-induced fish mortality, highlighting the critical need for developing genetically improved hypoxia-tolerant strains. In this study, a genome-wide association study (GWAS) was performed to unveil single-nucleotide polymorphisms (SNPs) associated with acute hypoxia tolerance and elucidate underlying regulatory mechanisms. A total of 382 P. olivaceus fish were measured for blood serum cortisol level using enzyme-linked immunosorbent assay (ELISA) upon acute hypoxia stress. Blood plasma cortisol levels were used as a proxy phenotype of hypoxia tolerance, with the hypothesis that elevated concentrations indicate low tolerance, and minimized concentrations indicate high tolerance to acute hypoxia stress. Then, fish were genotyped using the Affymetrix® Axiom® myDesign™ genotype array, and 57,651 SNPs were retained after quality control filtering. A significant SNP was identified on chromosome 15 of the P. olivaceus genome, above the suggestive threshold (p < 1 × 10⁻⁴) for the stress cortisol level trait. A strongly associated SNP with accession number AX-419149718 was located in the zona pellucida-like domain-containing protein 1 (Zpld1) gene. Gene ontology (GO) analysis suggests that Zpld1 is involved in signaling pathways, including the integrin-mediated signaling pathway, thereby contributing to the mitigation of hypoxia stress effects. Collectively, this study provides insights into the genetic basis of acute hypoxia tolerance in P. olivaceus and may serve as a foundation for establishing genomic selection of olive flounders with acute hypoxia stress tolerance in aquaculture breeding programs.

由水中溶解氧（DO）水平降低引起的缺氧是水产养殖中的一个关键挑战，经常导致鱼类应激、厌食和大量死亡。橄榄比目鱼（palichthys olivaceus）是韩国全球水产养殖出口的关键品种。然而，由于缺氧导致鱼类死亡，olivaceus的水产养殖遭受了重大的经济损失，因此迫切需要开发基因改良的耐缺氧菌株。在这项研究中，进行了一项全基因组关联研究（GWAS），揭示了与急性缺氧耐受性相关的单核苷酸多态性（snp），并阐明了潜在的调控机制。采用酶联免疫吸附法（ELISA）测定了382条橄榄鱼急性缺氧应激时的血清皮质醇水平。血浆皮质醇水平被用作低氧耐受性的代用表型，假设浓度升高表明低耐受性，而浓度最小化表明对急性缺氧应激的高耐受性。然后，使用Affymetrix®Axiom®myDesign™基因型阵列对鱼进行基因分型，质量控制过滤后保留了57,651个snp。在p . olivaceus基因组的第15号染色体上发现了一个显著的SNP，高于应激皮质醇水平性状的提示阈值（p < 1 × 10−4）。在透明带样结构域蛋白1 （Zpld1）基因中发现了一个与加入号AX-419149718密切相关的SNP。基因本体论（GO）分析表明，Zpld1参与信号通路，包括整合素介导的信号通路，从而有助于减轻缺氧胁迫的影响。总的来说，本研究提供了橄榄比目鱼急性缺氧耐受性的遗传基础，并可为在水产养殖育种计划中建立具有急性缺氧胁迫耐受性的橄榄比目鱼基因组选择奠定基础。

{"title":"Genome-wide association study of tolerance to acute hypoxia in the olive flounder (Paralichthys olivaceus) using individual blood cortisol levels as a physiological phenotype","authors":"M.A.H. Dilshan , Gaeun Kim , W.K.M. Omeka , D.S. Liyanage , H.M.V. Udayantha , D.C.G. Rodrigo , G.A.N.P. Ganepola , H.A.C.R. Hanchapola , Y.K. Kodagoda , Po Gong , Jihun Lee , Sukkyoung Lee , Jeongeun Kim , Jehee Lee","doi":"10.1016/j.gene.2025.149952","DOIUrl":"10.1016/j.gene.2025.149952","url":null,"abstract":"<div><div>Hypoxia, caused by reduced dissolved oxygen (DO) levels in water, is a critical challenge in aquaculture and often results in stress, anorexia, and mass mortality in fish. The olive flounder (<em>Paralichthys olivaceus</em>) is a key species in South Korea’s global aquaculture exports. However, aquaculture production of <em>P. olivaceus</em> experiences significant economic losses caused by hypoxia-induced fish mortality, highlighting the critical need for developing genetically improved hypoxia-tolerant strains. In this study, a genome-wide association study (GWAS) was performed to unveil single-nucleotide polymorphisms (SNPs) associated with acute hypoxia tolerance and elucidate underlying regulatory mechanisms. A total of 382 <em>P. olivaceus</em> fish were measured for blood serum cortisol level using enzyme-linked immunosorbent assay (ELISA) upon acute hypoxia stress. Blood plasma cortisol levels were used as a proxy phenotype of hypoxia tolerance, with the hypothesis that elevated concentrations indicate low tolerance, and minimized concentrations indicate high tolerance to acute hypoxia stress. Then, fish were genotyped using the Affymetrix® Axiom® myDesign™ genotype array, and 57,651 SNPs were retained after quality control filtering. A significant SNP was identified on chromosome 15 of the <em>P. olivaceus</em> genome, above the suggestive threshold (<em>p</em> < 1 × 10<sup>−4</sup>) for the stress cortisol level trait. A strongly associated SNP with accession number AX-419149718 was located in the zona pellucida-like domain-containing protein 1 (<em>Zpld1</em>) gene. Gene ontology (GO) analysis suggests that <em>Zpld1</em> is involved in signaling pathways, including the integrin-mediated signaling pathway, thereby contributing to the mitigation of hypoxia stress effects. Collectively, this study provides insights into the genetic basis of acute hypoxia tolerance in <em>P. olivaceus</em> and may serve as a foundation for establishing genomic selection of olive flounders with acute hypoxia stress tolerance in aquaculture breeding programs.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"980 ","pages":"Article 149952"},"PeriodicalIF":2.4,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145735922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential genomic signatures of homozygosity and a haplotype sharing Extents across breeds associated with body size variation in Korean indigenous goats 韩国本土山羊体型变异与品种间纯合子性和单倍型共享程度的差异基因组特征。

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-11 DOI: 10.1016/j.gene.2025.149956

Seongmin Kim , Hankyeol Jeong , Ga-Eun Kim , Kwan-Woo Kim , Woncheoul Park , Jaemin Kim , Bong-Hwan Choi

Korean indigenous goats are maintained as purebred lineages in geographically isolated populations such as Dangjin, Gyeongsang National University (GNU), Jangsu, and Tongyeong. However, their small population size and high rates of inbreeding have raised concerns regarding the preservation of genetic diversity. We generated SNP genotypes for 217 Korean indigenous goats using the GoatSNP50 BeadChip, and standardized body size measurements with complete metadata were available for 64 adult individuals. Principal component analysis (PCA) clearly separated the four lineages, with the GNU population showing particularly high values of inbreeding coefficient and proportion of runs of homozygosity, reflecting the impact of recent closed breeding. Genome-wide patterns of haplotype sharing revealed exploratory trends suggesting that introgression from global breeds tended to coincide with larger body size, whereas intensified inbreeding within the Korean population showed a general tendency toward reduced body size. Furthermore, cross-population extended haplotype homozygosity (XP-EHH) analysis revealed candidate genes, including ADGRL3, SP8, and ARL6IP5 that are likely involved in adaptation to seasonal environmental stress. Our findings highlight the global connectivity, functional relevance of body conformation traits, and selective signatures of Korean indigenous goats, providing a genomic foundation for preserving diversity and guiding future breeding.

在唐津、庆尚国立大学、长寿、统营等地理上孤立的地区，韩国本土山羊被维持为纯种。然而，它们的小种群规模和高近交率引起了人们对遗传多样性保护的关注。我们使用GoatSNP50 BeadChip对217只韩国本土山羊进行了SNP基因型分析，并对64只成年山羊进行了具有完整元数据的标准化体型测量。主成分分析（PCA）对4个世系进行了明显的分离，发现GNU群体的近交系系数和纯合子居次比例特别高，反映了近期封闭育种的影响。单倍型共享的全基因组模式揭示了探索性趋势，表明来自全球品种的基因渗入倾向于体型更大，而韩国种群内近亲繁殖的加剧则显示出体型变小的总体趋势。此外，跨群体扩展单倍型纯合分析（XP-EHH）揭示了候选基因，包括ADGRL3， SP8和ARL6IP5，可能参与适应季节性环境胁迫。我们的研究结果突出了韩国本土山羊的全球连通性、身体构象特征的功能相关性和选择性特征，为保护多样性和指导未来的育种提供了基因组基础。

{"title":"Differential genomic signatures of homozygosity and a haplotype sharing Extents across breeds associated with body size variation in Korean indigenous goats","authors":"Seongmin Kim , Hankyeol Jeong , Ga-Eun Kim , Kwan-Woo Kim , Woncheoul Park , Jaemin Kim , Bong-Hwan Choi","doi":"10.1016/j.gene.2025.149956","DOIUrl":"10.1016/j.gene.2025.149956","url":null,"abstract":"<div><div>Korean indigenous goats are maintained as purebred lineages in geographically isolated populations such as Dangjin, Gyeongsang National University (GNU), Jangsu, and Tongyeong. However, their small population size and high rates of inbreeding have raised concerns regarding the preservation of genetic diversity. We generated SNP genotypes for 217 Korean indigenous goats using the GoatSNP50 BeadChip, and standardized body size measurements with complete metadata were available for 64 adult individuals. Principal component analysis (PCA) clearly separated the four lineages, with the GNU population showing particularly high values of inbreeding coefficient and proportion of runs of homozygosity, reflecting the impact of recent closed breeding. Genome-wide patterns of haplotype sharing revealed exploratory trends suggesting that introgression from global breeds tended to coincide with larger body size, whereas intensified inbreeding within the Korean population showed a general tendency toward reduced body size. Furthermore, cross-population extended haplotype homozygosity (XP-EHH) analysis revealed candidate genes, including <em>ADGRL3</em>, <em>SP8</em>, and <em>ARL6IP5</em> that are likely involved in adaptation to seasonal environmental stress. Our findings highlight the global connectivity, functional relevance of body conformation traits, and selective signatures of Korean indigenous goats, providing a genomic foundation for preserving diversity and guiding future breeding.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"980 ","pages":"Article 149956"},"PeriodicalIF":2.4,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145752089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inorganic nitrogen sources and light cooperatively stimulate gene regulation for non-photosynthetic phosphoenolpyruvate carboxylase in wheat leaves 无机氮源和光协同刺激小麦叶片非光合磷酸化烯醇丙酮酸羧化酶的基因调控。

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-09 DOI: 10.1016/j.gene.2025.149950

Naoki Yamamoto , Jingru Jiang , Haoyu Deng , Ken-ichi Kurotani , Jiang Zou , Zaijun Yang

Phosphoenolpyruvate carboxylase (PEPC) is a bicarbonate fixation enzyme that is associated with nitrogen metabolism. We previously observed that NO₃^– affected the expression levels of PEPC genes differently in detached wheat leaves. Three types of PEPC isogenes, including the orthologous isogene group (Tappc1b) for C₄-photosynthetic PEPC, exhibited distinct transcriptionally up-regulated patterns from one another. However, it remains unclear which nitrogen compounds exactly trigger the gene expression responses and their light dependency. Here, we investigated the cause of isogene-specific expression responses of PEPCs to different forms of nitrogen supplementation. Detached leaves from wheat seedlings cultivated under a nitrogen-deficient condition were incubated with NO₃^–, NH₄⁺, and urea under both light and dark conditions. Notably, the genes analyzed exhibited markedly different expression patterns across the nitrogen forms under the light condition. Tappc1a, which did non-respond to NO₃^–, was up-regulated by NH₄⁺ exclusively under light. Tappc2 was also up-regulated by NO₃^– and NH₄⁺ only under light, and these up-regulations were suppressed under the dark condition. The candidate transcriptional regulator DOF1 for Tappc1b in wheat was also up-regulated by NO₃^– only under light condition, but earlier than Tappc1b, implicating a DOF1-mediated transcriptional response of Tappc1b to NO₃^–. Comparison of Tappc1b promoter sequences with that of NO₃^–-responsive maize C₄ PEPC revealed conserved light-responsive elements near the Dof1 binding site. These findings suggest that distinct transcriptional regulatory cascades would exist for non-photosynthetic PEPCs in response to different inorganic nitrogen forms.

磷酸烯醇丙酮酸羧化酶（PEPC）是一种与氮代谢有关的碳酸氢盐固定酶。NO3-对小麦离体叶片PEPC基因表达水平的影响存在差异。三种类型的PEPC同基因，包括c4 -光合PEPC的同源同基因组（Tappc1b），表现出不同的转录上调模式。然而，目前尚不清楚究竟是哪种氮化合物触发了基因表达反应及其对光的依赖性。在这里，我们研究了PEPCs对不同形式补氮的等基因特异性表达反应的原因。采用NO3-、NH4+和尿素对缺氮小麦幼苗离体叶片进行光照和暗培养。值得注意的是，在光照条件下，所分析的基因在不同氮形态下表现出明显不同的表达模式。对NO3-无反应的Tappc1a在光照条件下仅被NH4+上调。光照条件下，NO3-和NH4+也上调了Tappc2，而在黑暗条件下，这些上调被抑制。光照条件下，小麦中调控Tappc1b的候选转录调控因子DOF1也仅被NO3-上调，但上调时间早于Tappc1b，提示Tappc1b对NO3-的转录响应是由DOF1介导的。将Tappc1b启动子序列与NO3响应玉米C4PEPC的启动子序列进行比较，发现Dof1结合位点附近有保守的光响应元件。这些发现表明，非光合作用的PEPCs在对不同无机氮形式的响应中可能存在不同的转录调控级联。

{"title":"Inorganic nitrogen sources and light cooperatively stimulate gene regulation for non-photosynthetic phosphoenolpyruvate carboxylase in wheat leaves","authors":"Naoki Yamamoto , Jingru Jiang , Haoyu Deng , Ken-ichi Kurotani , Jiang Zou , Zaijun Yang","doi":"10.1016/j.gene.2025.149950","DOIUrl":"10.1016/j.gene.2025.149950","url":null,"abstract":"<div><div>Phospho<em>enol</em>pyruvate carboxylase (PEPC) is a bicarbonate fixation enzyme that is associated with nitrogen metabolism. We previously observed that NO<sub>3</sub><sup>–</sup> affected the expression levels of <em>PEPC</em> genes differently in detached wheat leaves. Three types of <em>PEPC</em> isogenes, including the orthologous isogene group (<em>Tappc1b</em>) for C<sub>4</sub>-photosynthetic <em>PEPC</em>, exhibited distinct transcriptionally up-regulated patterns from one another. However, it remains unclear which nitrogen compounds exactly trigger the gene expression responses and their light dependency. Here, we investigated the cause of isogene-specific expression responses of <em>PEPCs</em> to different forms of nitrogen supplementation. Detached leaves from wheat seedlings cultivated under a nitrogen-deficient condition were incubated with NO<sub>3</sub><sup>–</sup>, NH<sub>4</sub><sup>+</sup>, and urea under both light and dark conditions. Notably, the genes analyzed exhibited markedly different expression patterns across the nitrogen forms under the light condition. <em>Tappc1a</em>, which did non-respond to NO<sub>3</sub><sup>–</sup>, was up-regulated by NH<sub>4</sub><sup>+</sup> exclusively under light. <em>Tappc2</em> was also up-regulated by NO<sub>3</sub><sup>–</sup> and NH<sub>4</sub><sup>+</sup> only under light, and these up-regulations were suppressed under the dark condition. The candidate transcriptional regulator <em>DOF1</em> for <em>Tappc1b</em> in wheat was also up-regulated by NO<sub>3</sub><sup>–</sup> only under light condition, but earlier than <em>Tappc1b</em>, implicating a DOF1-mediated transcriptional response of <em>Tappc1b</em> to NO<sub>3</sub><sup>–</sup>. Comparison of <em>Tappc1b</em> promoter sequences with that of NO<sub>3</sub><sup>–</sup>-responsive maize C<sub>4</sub> <em>PEPC</em> revealed conserved light-responsive elements near the Dof1 binding site. These findings suggest that distinct transcriptional regulatory cascades would exist for non-photosynthetic <em>PEPCs</em> in response to different inorganic nitrogen forms.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"980 ","pages":"Article 149950"},"PeriodicalIF":2.4,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145741963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene therapy for Krabbe disease: evidence from mouse and canine models 克拉伯病的基因治疗：来自小鼠和犬模型的证据。

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-09 DOI: 10.1016/j.gene.2025.149949

Yang Li , Xiang Dong , Jian Guo , Ya-feng Lv

Globoid cell leukodystrophy (GLD) is an autosomal recessive lysosomal storage disorder caused by mutations in the β-galactosylceramidase (GALC) gene, resulting in enzyme deficiency and the progressive accumulation of galactosylsphingosine and galactosylceramide in the white matter of the central nervous system and in peripheral nerves, which in turn triggers demyelination. Although no curative therapy is currently available, studies in animal models in recent years have shown that gene therapy can ameliorate pathological and biochemical abnormalities and holds considerable promise for clinical translation. This article reviews advances in gene therapy in animal models of GLD and discusses key directions and challenges for future treatments.

Globoid cell leukodystrophy （GLD）是一种常染色体隐性溶酶体贮积症，由β-半乳糖神经酰胺酶（GALC）基因突变引起，导致中枢神经系统白质和周围神经中半乳糖神经酰胺和半乳糖神经酰胺的酶缺乏和进行性积累，进而引发脱髓鞘。虽然目前还没有治愈性的治疗方法，但近年来在动物模型上的研究表明，基因治疗可以改善病理和生化异常，并且在临床转化方面具有相当大的前景。本文综述了GLD动物模型基因治疗的进展，并讨论了未来治疗的关键方向和挑战。

引用次数: 0

Comprehensive transcriptomic profiling reveals lncRNA–miRNA–mRNA regulatory networks in skeletal muscle aging of mice 综合转录组学分析揭示了小鼠骨骼肌衰老中的lncRNA-miRNA-mRNA调控网络。

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-08 DOI: 10.1016/j.gene.2025.149946

Jinrui Jia, Qingyan Wang, Xuanye Jiang, Hao Chen, Minwei Huang, Bing Ni, Huiying Zhang, Xin’e Shi, Jianjun Jin

Purpose

As organisms age, physiological and pathological changes occur, with altered lncRNA expression playing a key role. However, their regulatory mechanisms in aging remain unclear. This study investigates the differential expression of lncRNAs between aged and young mice, and explores the lncRNA–miRNA–mRNA interplay to gain insights into the molecular basis of aging.

Methods

We performed whole-transcriptome sequencing on tibialis anterior muscles from four aged (20-month-old) and four young (3-month-old) mice. Hub genes were identified via PPI and WGCNA analyses, followed by functional enrichment. Integrative analysis revealed interactions among differentially expressed lncRNAs, miRNAs, and mRNAs, leading to the construction of cis-/trans-regulatory and ceRNA networks.

Results

Our results revealed 746 significantly differentially expressed known lncRNAs (465 upregulated, 281 downregulated) and 27 novel lncRNAs in aged mouse TA muscle, alongside 50 miRNAs and 1124 mRNAs. Based on lncRNA classification (antisense, intergenic, intronic), we constructed subtype-specific cis- and trans-regulatory networks. Hub genes were identified via PPI and WGCNA analyses to further refine these networks. Highly expressed and variable genes were also integrated into regulatory mapping. Enrichment analyses indicated involvement in extracellular matrix remodeling, epithelial cell migration, and immune response.

Conclusions

This study systematically profiled age-related changes in lncRNAs, miRNAs, and mRNAs in TA muscle, and constructed core regulatory networks based on lncRNA subtypes. This study systematically profiled age-related transcriptomic changes in mouse skeletal muscle and constructed lncRNA–miRNA–mRNA regulatory networks associated with aging. These results provide a valuable resource and generate hypotheses for future experimental validation of lncRNA-mediated regulatory mechanisms in muscle aging.

目的：随着生物年龄的增长，生理病理发生变化，lncRNA表达的改变起着关键作用。然而，它们在衰老中的调节机制尚不清楚。本研究通过研究老年小鼠和幼龄小鼠lncrna的差异表达，探讨lncRNA-miRNA-mRNA的相互作用，从而深入了解衰老的分子基础。方法：我们对4只成年（20月龄）和4只幼年（3月龄）小鼠的胫骨前肌进行了全转录组测序。通过PPI和WGCNA分析鉴定Hub基因，然后进行功能富集。整合分析揭示了差异表达的lncrna、mirna和mrna之间的相互作用，导致顺式/反式调控和ceRNA网络的构建。结果：我们的研究结果揭示了746个已知lncrna（465个上调，281个下调）和27个新lncrna在老年小鼠TA肌中显著表达差异，以及50个mirna和1124个mrna。基于lncRNA分类（反义、基因间、内含子），我们构建了亚型特异性的顺式和反式调控网络。通过PPI和WGCNA分析鉴定枢纽基因，进一步完善这些网络。高表达基因和可变基因也被整合到调控图谱中。富集分析表明参与细胞外基质重塑、上皮细胞迁移和免疫应答。结论：本研究系统分析了TA肌中lncRNA、mirna和mrna的年龄相关变化，并构建了基于lncRNA亚型的核心调控网络。本研究系统分析了小鼠骨骼肌中与年龄相关的转录组变化，构建了与衰老相关的lncRNA-miRNA-mRNA调控网络。这些结果为未来实验验证lncrna介导的肌肉衰老调控机制提供了宝贵的资源和假设。

{"title":"Comprehensive transcriptomic profiling reveals lncRNA–miRNA–mRNA regulatory networks in skeletal muscle aging of mice","authors":"Jinrui Jia, Qingyan Wang, Xuanye Jiang, Hao Chen, Minwei Huang, Bing Ni, Huiying Zhang, Xin’e Shi, Jianjun Jin","doi":"10.1016/j.gene.2025.149946","DOIUrl":"10.1016/j.gene.2025.149946","url":null,"abstract":"<div><h3>Purpose</h3><div>As organisms age, physiological and pathological changes occur, with altered lncRNA expression playing a key role. However, their regulatory mechanisms in aging remain unclear. This study investigates the differential expression of lncRNAs between aged and young mice, and explores the lncRNA–miRNA–mRNA interplay to gain insights into the molecular basis of aging.</div></div><div><h3>Methods</h3><div>We performed whole-transcriptome sequencing on tibialis anterior muscles from four aged (20-month-old) and four young (3-month-old) mice. Hub genes were identified via PPI and WGCNA analyses, followed by functional enrichment. Integrative analysis revealed interactions among differentially expressed lncRNAs, miRNAs, and mRNAs, leading to the construction of cis-/<em>trans</em>-regulatory and ceRNA networks.</div></div><div><h3>Results</h3><div>Our results revealed 746 significantly differentially expressed known lncRNAs (465 upregulated, 281 downregulated) and 27 novel lncRNAs in aged mouse TA muscle, alongside 50 miRNAs and 1124 mRNAs. Based on lncRNA classification (antisense, intergenic, intronic), we constructed subtype-specific cis- and <em>trans</em>-regulatory networks. Hub genes were identified via PPI and WGCNA analyses to further refine these networks. Highly expressed and variable genes were also integrated into regulatory mapping. Enrichment analyses indicated involvement in extracellular matrix remodeling, epithelial cell migration, and immune response.</div></div><div><h3>Conclusions</h3><div>This study systematically profiled age-related changes in lncRNAs, miRNAs, and mRNAs in TA muscle, and constructed core regulatory networks based on lncRNA subtypes. This study systematically profiled age-related transcriptomic changes in mouse skeletal muscle and constructed lncRNA–miRNA–mRNA regulatory networks associated with aging. These results provide a valuable resource and generate hypotheses for future experimental validation of lncRNA-mediated regulatory mechanisms in muscle aging.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"979 ","pages":"Article 149946"},"PeriodicalIF":2.4,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145722080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target of rapamycin (TOR) kinases in Leishmania: Insights from comparative analyses with Trypanosomatids 雷帕霉素（TOR）激酶在利什曼原虫中的靶标：来自与锥虫病比较分析的见解。

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-07 DOI: 10.1016/j.gene.2025.149933

Soumi Chowdhury , Harsh Pawar

The Target of Rapamycin (TOR) kinase family is a central regulator of eukaryotic cell growth and metabolism. Unlike most eukaryotes that possess one or two TOR genes, Leishmania species encode four distinct paralogs, suggesting lineage-specific expansion and functional diversification. In this study, we performed a comprehensive phylogenetic and domain analysis of TOR paralogs across multiple Leishmania species, with Trypanosoma brucei serving as a comparative reference. TOR1 and TOR2 were found to be highly conserved, possessing canonical FAT, FRB, and PI3Kc domains, consistent with their roles in the essential TORC1 and TORC2 complexes. TOR3 and TOR4 displayed significant sequence divergence and altered domain structures, particularly in visceral and mucocutaneous species. TOR3 lacks the FRB domain but retains kinase activity and is implicated in arginine sensing and acidocalcisome biogenesis. TOR4 shows the highest divergence, including truncated domains and species-specific clustering, suggesting a role in parasite adaptation or stage differentiation. Functional annotations further support this, as TOR1 and TOR2 are enriched in kinase functions, while TOR3 and TOR4 are associated with hypothetical or uncharacterized proteins. The conserved PI3Kc domain across all paralogs offers a target for drug development. These findings enhance our understanding of TOR evolution and its therapeutic potential in leishmaniasis.

雷帕霉素靶蛋白（TOR）激酶家族是真核细胞生长和代谢的中心调节因子。与大多数拥有一个或两个TOR基因的真核生物不同，利什曼原虫物种编码四个不同的类似物，表明谱系特异性扩展和功能多样化。在这项研究中，我们对多个利什曼原虫物种的TOR类似性进行了全面的系统发育和结构域分析，并以布鲁氏锥虫作为比较参考。TOR1和TOR2是高度保守的，具有典型的FAT、FRB和PI3Kc结构域，这与它们在TORC1和TORC2复合物中的作用一致。TOR3和TOR4表现出明显的序列分化和结构域结构改变，特别是在内脏和粘膜皮肤物种中。TOR3缺乏FRB结构域，但保留激酶活性，并与精氨酸感知和酸钙酶体的生物发生有关。TOR4表现出最高的分化，包括截断的结构域和物种特异性聚类，表明其在寄生虫适应或阶段分化中起作用。功能注释进一步支持了这一点，因为TOR1和TOR2富含激酶功能，而TOR3和TOR4与假设的或未表征的蛋白质相关。保守的PI3Kc结构域在所有类似物中都为药物开发提供了一个靶点。这些发现增强了我们对TOR进化及其治疗利什曼病潜力的理解。

{"title":"Target of rapamycin (TOR) kinases in Leishmania: Insights from comparative analyses with Trypanosomatids","authors":"Soumi Chowdhury , Harsh Pawar","doi":"10.1016/j.gene.2025.149933","DOIUrl":"10.1016/j.gene.2025.149933","url":null,"abstract":"<div><div>The Target of Rapamycin (TOR) kinase family is a central regulator of eukaryotic cell growth and metabolism. Unlike most eukaryotes that possess one or two TOR genes, <em>Leishmania</em> species encode four distinct paralogs, suggesting lineage-specific expansion and functional diversification. In this study, we performed a comprehensive phylogenetic and domain analysis of TOR paralogs across multiple <em>Leishmania</em> species, with <em>Trypanosoma brucei</em> serving as a comparative reference. TOR1 and TOR2 were found to be highly conserved, possessing canonical FAT, FRB, and PI3Kc domains, consistent with their roles in the essential TORC1 and TORC2 complexes. TOR3 and TOR4 displayed significant sequence divergence and altered domain structures, particularly in visceral and mucocutaneous species. TOR3 lacks the FRB domain but retains kinase activity and is implicated in arginine sensing and acidocalcisome biogenesis. TOR4 shows the highest divergence, including truncated domains and species-specific clustering, suggesting a role in parasite adaptation or stage differentiation. Functional annotations further support this, as TOR1 and TOR2 are enriched in kinase functions, while TOR3 and TOR4 are associated with hypothetical or uncharacterized proteins. The conserved PI3Kc domain across all paralogs offers a target for drug development. These findings enhance our understanding of TOR evolution and its therapeutic potential in leishmaniasis.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"979 ","pages":"Article 149933"},"PeriodicalIF":2.4,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145713995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DoWRKY26 positively regulating flavonoid biosynthesis in Dendrobium officinale dowky26正调控铁皮石斛类黄酮生物合成。

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-06 DOI: 10.1016/j.gene.2025.149944

Ziling Tao , Shiyu Cai , YiMing Wang, Fengxiu li, Lu Lv, Haimeng Bai, Ludan Li, Jihong Jiang, Xiaoying Cao

Dendrobium officinale is renowned as the foremost among the “Nine Immortal Herbs of China”. Our previous research showed enhanced flavonoid accumulation following induction by the endophyte Wickerhamomyces sp. KLBMPSYLp8. Transcriptome data analysis identified multiple upregulated transcription factor (TF) genes. We conducted transient overexpression analysis of 10 significantly upregulated TF genes in D. officinale leaves. The results demonstrated that transient overexpression of the DoWRKY26 significantly enhanced flavonoid accumulation, with a 33 % increase compared to the empty vector control group. Furthermore, DoWRKY26 overexpression also upregulated the expression levels of key enzyme genes implicated in the flavonoid biosynthesis pathway. Subcellular localization confirmed its nuclear presence. DoWRKY26 expression was induced by salicylic acid (SA), abscisic acid (ABA), 1-Aminocyclopropane-1-carboxylic Acid (ACC) and methyl jasmonate (MeJA). Yeast one-hybrid (Y1H), Electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter assays verified that DoWRKY26 could bind and activate the promoter of DoCCoAOMT. These findings provide a foundational basis for further exploring the biosynthesis and transcriptional regulation mechanisms of flavonoids in D. officinale.

铁皮石斛被誉为“中国九仙”之首。我们之前的研究表明，内生菌Wickerhamomyces sp. KLBMPSYLp8诱导后，黄酮类化合物的积累增强。转录组数据分析发现多个转录因子（TF）基因上调。我们对10个显著上调的TF基因在铁皮草叶片中进行了瞬时过表达分析。结果表明，瞬时过表达dowky26显著增强了黄酮类化合物的积累，与空载体对照组相比，增加了33 %。此外，dowky26的过表达还上调了与类黄酮生物合成途径相关的关键酶基因的表达水平。亚细胞定位证实了其核的存在。水杨酸（SA）、脱落酸（ABA）、1-氨基环丙烷-1-羧酸（ACC）和茉莉酸甲酯（MeJA）诱导dowky26表达。酵母单杂交（Y1H）、电泳迁移率转移试验（EMSA）和双荧光素酶报告子试验证实，dowky26可以结合并激活DoCCoAOMT的启动子。这些发现为进一步探索铁皮石斛黄酮类化合物的生物合成及转录调控机制提供了基础。

{"title":"DoWRKY26 positively regulating flavonoid biosynthesis in Dendrobium officinale","authors":"Ziling Tao , Shiyu Cai , YiMing Wang, Fengxiu li, Lu Lv, Haimeng Bai, Ludan Li, Jihong Jiang, Xiaoying Cao","doi":"10.1016/j.gene.2025.149944","DOIUrl":"10.1016/j.gene.2025.149944","url":null,"abstract":"<div><div><em>Dendrobium officinale</em> is renowned as the foremost among the “Nine Immortal Herbs of China”. Our previous research showed enhanced flavonoid accumulation following induction by the endophyte <em>Wickerhamomyces</em> sp. KLBMPSYLp8. Transcriptome data analysis identified multiple upregulated transcription factor (TF) genes. We conducted transient overexpression analysis of 10 significantly upregulated TF genes in <em>D. officinale</em> leaves. The results demonstrated that transient overexpression of the <em>DoWRKY26</em> significantly enhanced flavonoid accumulation, with a 33 % increase compared to the empty vector control group. Furthermore, <em>DoWRKY26</em> overexpression also upregulated the expression levels of key enzyme genes implicated in the flavonoid biosynthesis pathway. Subcellular localization confirmed its nuclear presence. <em>DoWRKY26</em> expression was induced by salicylic acid (SA), abscisic acid (ABA), 1-Aminocyclopropane-1-carboxylic Acid (ACC) and methyl jasmonate (MeJA). Yeast one-hybrid (Y1H), Electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter assays verified that DoWRKY26 could bind and activate the promoter of <em>DoCCoAOMT</em>. These findings provide a foundational basis for further exploring the biosynthesis and transcriptional regulation mechanisms of flavonoids in <em>D. officinale</em>.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"979 ","pages":"Article 149944"},"PeriodicalIF":2.4,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145707083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editors’ Corner: Suppressor of cytokine signaling 6 (SOCS6) mediates ubiquitination-dependent degradation of SLC7A11 to promote ferroptosis in ovarian cancer 细胞因子信号传导抑制因子6 （SOCS6）介导SLC7A11泛素化依赖性降解，促进卵巢癌铁下垂。

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene

Pub Date : 2025-12-06 DOI: 10.1016/j.gene.2025.149937

Xavier Graña

引用次数: 0

Gene最新文献

Purpose

Methods

Results

Conclusions