Future Virology最新文献

{"title":"Oral bemnifosbuvir (AT-527) vs placebo in patients with mild-to-moderate COVID-19 in an outpatient setting (MORNINGSKY).","authors":"Arantxa Horga, Rebecca Saenz, Gürdal Yilmaz, Abraham Simón-Campos, Keith Pietropaolo, William J Stubbings, Neil Collinson, Laura Ishak, Barbara Zrinscak, Bruce Belanger, Catherine Granier, Kai Lin, Aeron C Hurt, Xiao-Jian Zhou, Steffen Wildum, Janet Hammond","doi":"10.2217/fvl-2023-0115","DOIUrl":"10.2217/fvl-2023-0115","url":null,"abstract":"<p><p><b>Aim:</b> This phase III study assessed the efficacy/safety/antiviral activity/pharmacokinetics of bemnifosbuvir, a novel, oral nucleotide analog to treat COVID-19. <b>Patients & methods:</b> Outpatient adults/adolescents with mild-to-moderate COVID-19 were randomized 2:1 to bemnifosbuvir/placebo. Time to symptom alleviation/improvement (primary outcome), risk of hospitalization/death, viral load and safety were evaluated. <b>Results:</b> Although the study was discontinued prematurely and did not meet its primary end point, bemnifosbuvir treatment resulted in fewer hospitalizations (71% relative risk reduction), COVID-19-related medically attended hospital visits, and COVID-19-related complications compared with placebo. No reduction in viral load was observed. The proportion of patients with adverse events was similar; no deaths occurred. <b>Conclusion:</b> Bemnifosbuvir showed hospitalization reduction in patients with variable disease progression risk and was well tolerated. <b>Clinical Trial Registration</b>: NCT04889040 (ClinicalTrials.gov).</p>","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71480363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of human papillomavirus type 197 genome in non-melanoma skin cancer and adjacent non-tumoral skin margins","authors":"Zeinab Vosough, Farzin Sadeghi, G. Kamrani, A. Hasanzadeh, Mohammad Ranaee","doi":"10.2217/fvl-2023-0022","DOIUrl":"https://doi.org/10.2217/fvl-2023-0022","url":null,"abstract":"Aim: We aimed to investigate the presence of human papillomavirus type 197 (HPV197) in patients with cutaneous squamous cell carcinoma (SCC) and basal cell carcinoma (BCC). Materials & methods: 58 formalin-fixed paraffin-embedded tissue samples, including 12 SCC and 46 BCC and the adjacent non-tumoral skin of the same patient were analyzed for the presence of HPV197E6 gene, using a quantitative real-time PCR assay. Results: The HPV197E6 gene was identified in 12 tumor samples (7 BCC and 5 SCC), but not in the control group. Conclusion: Based on these results, a high copy number of HPV197E6 gene provides additional support for the role of HPV197 in skin cancer.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47928648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first evidence of Seoul hantavirus, hepatitis E virus and rabies virus in Rattus norvegicus in Tehran, Iran","authors":"T. Azimi, Sina Nasrollahian, S. Sabour, Nahal Hadi, L. Azimi, N. Rahbarian, A. Karimi, F. Fallah, Roxana Mansour-Ghanaie, S. Hoseini-Alfatemi, S. A. Fahimzad","doi":"10.2217/fvl-2023-0047","DOIUrl":"https://doi.org/10.2217/fvl-2023-0047","url":null,"abstract":"Aim: The present study aimed to investigate the frequency of Seoul hantavirus (SEOV), Hepatitis E and rabies viruses in Norway rats in Tehran, Iran. Methods: With the aid of a rat ELISA kit, the we identified specific IgG antibodies against the Rabies virus. The presence of SEOV and HEV was determined using an SYBR Green-based real-time PCR assay. Results: 1% of the R. norvegicus carried the rabies virus. HEV was associated with R. norvegicus obtained from the south of Tehran with the highest frequency (35%). 12% of tests for SEOV were positive. Conclusion: The findings highlight the importance of putting in place rodent control programs and avoiding interaction with rodent populations in urban settings.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42547487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an in vitro model to study hepatitis C virus-induced fibrosis","authors":"Bisma Rauff, Mehdi Ramezani-Moghadam, Enoch S. E. Tay, Jacob George, M. Douglas","doi":"10.2217/fvl-2022-0206","DOIUrl":"https://doi.org/10.2217/fvl-2022-0206","url":null,"abstract":"Aim: To investigate the mechanism of liver fibrosis in chronic hepatitis C virus (HCV) infection we developed an in vitro model. Methods: Huh7 cells were transfected with HCV (JFH1 or Jc1) or sub-genomic replicons and expression of pro-fibrotic cytokines determined by qPCR. Media from infected cells was transferred onto LX2 cells or primary rat stellate cells and expression of pro-fibrotic genes measured by qPCR. Results: Replication competent HCV, but not sub-genomic replicons, induced expression of TGF-β1 and CTGF. Supernatant from infected cells induced α-SMA and COL1A1 expression and stellate cell migration, confirming functional activation. Conclusion: Infected cells produce pro-fibrogenic cytokines TGF-β1 and CTGF and activate stellate cells. This mechanism could be targeted to prevent or treat HCV-induced fibrosis.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68215671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yellow fever: is Asia bound to encounter the virus?","authors":"Baijayantimala Mishra, Sutapa Rath, Monalisa Mohanty, Prabhudutta Mamidi","doi":"10.2217/fvl-2023-0128","DOIUrl":"https://doi.org/10.2217/fvl-2023-0128","url":null,"abstract":"Future VirologyAhead of Print EditorialYellow fever: is Asia bound to encounter the virus?Baijayantimala Mishra, Sutapa Rath, Monalisa Mohanty & Prabhudutta MamidiBaijayantimala Mishra *Author for correspondence: Tel.: +91 943 888 4121; E-mail Address: bmishramicro@gmail.comhttps://orcid.org/0000-0002-2604-6678Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author, Sutapa Rath https://orcid.org/0000-0002-0790-1448Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author, Monalisa Mohanty https://orcid.org/0000-0003-3940-1998Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author & Prabhudutta Mamidi https://orcid.org/0000-0002-0187-571XDepartment of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this authorPublished Online:8 Sep 2023https://doi.org/10.2217/fvl-2023-0128AboutSectionsView ArticleView Full TextPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInRedditEmail View articleKeywords: aedes mosquitoAfricaarbovirusAsiatransmissionyellow feverPapers of special note have been highlighted as: • of interest; •• of considerable interestReferences1. Kallas EG, Wilder-Smith A. Managing severe yellow fever in the intensive care: lessons learnt from Brazil. J. Travel Med. 26(5), taz043 (2019). • Paper that describes the clinical spectrums of yellow fever.Crossref, Medline, Google Scholar2. Ho YL, Joelsons D, Leite GFC et al. Severe yellow fever in Brazil: clinical characteristics and management. J. Travel Med. 26(5), taz040 (2019). • A study that described the clinical management of severe yellow fever infection.Crossref, Medline, Google Scholar3. Cunha MDP, Duarte-Neto AN, Pour SZ et al. Phylogeographic patterns of the yellow fever virus around the metropolitan region of São Paulo, Brazil, 2016–2019. PLOS Negl. Trop. Dis. 16(9), e0010705 (2022).Crossref, Medline, CAS, Google Scholar4. Rodríguez-Morales AJ, Bonilla-Aldana DK, Suárez JA et al. Yellow fever reemergence in Venezuela - Implications for international travellers and Latin American countries during the COVID-19 pandemic. Travel Med. Infect. Dis. 44, 102192 (2021). • Article on importance of immunization coverage and optimization of surveillance system for populations at risk of yellow fever.Crossref, Medline, CAS, Google Scholar5. Gardner CL, Ryman KD. Yellow fever: a reemerging threat. Clin. Lab. Med. 30(1), 237–260 (2010).Crossref, Medline, Google Scholar6. Gubler DJ. Pandemic yellow fever: a potential threat to global health via travellers. J. Travel Med. 25(1), (2018). •• One of the earliest paper emphasising on the pandemic potential of yellow fever.Crossref, Medline, Google Scholar7. Song R, Guan S, Le","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136298523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultradiluted Eupatorium perfoliatum alleviates DENV-induced fibrosis by regulation of TGFβ1, MMP-9 and interferons","authors":"Avipsha Sarkar, Anirban Roy, Madhulina Maity, D. Nayak, Satadal Das","doi":"10.2217/fvl-2023-0121","DOIUrl":"https://doi.org/10.2217/fvl-2023-0121","url":null,"abstract":"Aim: This study aimed to alleviate dengue virus (DENV)-mediated fibrosis in a chick embryo model using ultradiluted Eupatorium perfoliatum (UEP). Materials & methods: Chorioallantoic membrane and liver of infected embryonated chicken eggs were checked for DENV pathogenesis. Cytotoxicity of UEP was assessed by MTT and scratch assay. Cytopathic assay after DENV infection was performed in vitro to check the effect of UEP and its constituents. Histopathology analysis revealed DENV-mediated fibrosis and the effect of UEP. qRT-PCR and gelatin zymography showed levels of fibrosis-causing genes. Results: UEP showed low cytotoxicity and maximum potential in mitigating DENV-mediated fibrosis in vivo via regulation of fibrosis biomarkers TGF-β, MMP-1, MMP-9, TIMP-1 and IFNs. Conclusion: UEP may be considered to alleviate DENV-mediated pathogenesis.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48930161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highlighting the role of immune responses, cell death and the nervous system in acute rotavirus infection using weighted gene co-expression network analysis","authors":"Saeed Samadizadeh, Ali Vaez, Z. Jamalpoor, A. Tahamtan","doi":"10.2217/fvl-2023-0045","DOIUrl":"https://doi.org/10.2217/fvl-2023-0045","url":null,"abstract":"Aim: Identifying genes and pathways involved in various stages of rotavirus infection could facilitate the mapping of the interactions between the virus and host. Materials & methods: Weighted gene co-expression network analysis (WGCNA) was employed to construct a co-expression network of genes identified by microarray analysis from samples from children with acute rotavirus infection. Results: Two specific modules were most related to rotavirus infection. The analysis of differentially expressed genes (DEGs) disclosed 393 DEGs between normal and infected individuals. Eighteen novel genes were shared between the modules. Conclusion: The WGCNA revealed novel modules, co-expressed genes and pathways involved in immune responses, cell death/degradation and the nervous system in acute rotavirus infection.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45271568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N6-methyladenosine methylation of viral genes","authors":"Jiting Sun, Yan Zhang, Bin Luo","doi":"10.2217/fvl-2022-0201","DOIUrl":"https://doi.org/10.2217/fvl-2022-0201","url":null,"abstract":"N6-methyladenosine (m6A) modification is the most pervasive type of RNA epigenetic modification in eukaryotes and the most common type of posttranscriptional modification of mRNA. m6A modification is a dynamic reversible process that can affect gene expression and play a crucial role in mRNA metabolism and multiple biological processes, ranging from RNA processing, nuclear export and RNA translation to decay. Meanwhile, much evidence has shown that m6A methylation regulates the life cycle of viruses and inhibits or promotes the development of various viruses. However, the mechanism of m6A methylation in virus-associated tumors has not been fully elucidated. This review highlights the role of m6A modification in various viruses to help establish new approaches for treating related diseases.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43203831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and evaluation of a multiplex PCR amplification-based sequencing method for respiratory virus type A","authors":"Junhong Luo, Yao Huang, Yan Guo, Zixinrong Meng, Kangchen Zhao, Xiaojuan Zhu, Le Zhou, Tao Wu, Qiao Qiao, Yiyue Ge, Qin Xu, Lunbiao Cui","doi":"10.2217/fvl-2023-0083","DOIUrl":"https://doi.org/10.2217/fvl-2023-0083","url":null,"abstract":"Aim: This study aimed to establish a simple whole-genome sequencing method for respiratory virus type A (RSVA) used in genomic epidemiological studies. Methods: Multiple primers were designed to amplify overlapping amplicons specific to RSVA. The amplicons were sequenced using Illumina and Nanopore sequencing platforms and the sequencing performances of the two platforms were also compared. Results: The study shows that the developed method could recover almost full genomes of RSVA when Ct values were less than 33 for clinical samples. Conclusion: In this study, a whole-genome sequencing method was developed for RSVA based on multiplex PCR.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135346818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral evaluation of 1,4-disubstituted-1,2,3-triazole derivatives against Chikungunya virus","authors":"Vitor Won-Held Rabelo, Verônica Diniz da Silva, Maria Leonisa Sanchez Nuñez, Leonardo dos Santos Corrêa Amorim, Camilla Djenne Buarque, Richard J Kuhn, Paula Alvarez Abreu, Izabel Christina Nunes de Palmer Paixão","doi":"10.2217/fvl-2023-0142","DOIUrl":"https://doi.org/10.2217/fvl-2023-0142","url":null,"abstract":"Aim: This work aimed to investigate the antiviral activity of two 1,4-disubstituted-1,2,3-triazole derivatives (1 and 2) against Chikungunya virus (CHIKV) replication. Materials &amp; methods: Cytotoxicity was analyzed using colorimetric assays and the antiviral potential was evaluated using plaque assays and computational tools. Results: Compound 2 showed antiviral activity against CHIKV 181-25 in BHK-21 and Vero cells. Also, this compound presented a higher activity against CHIKV BRA/RJ/18 in Vero cells, like compound 1. Compound 2 exhibited virucidal activity and inhibited virus entry while compound 1 inhibited virus release. Molecular docking suggested that these derivatives inhibit nsP1 protein while compound 1 may also target capsid protein. Conclusion: Both compounds exhibit promising antiviral activity against CHIKV by blocking different steps of virus replication.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}