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Identification of host factors that bind to the 5′ end of the MERS-CoV RNA genome 鉴定与MERS-CoV RNA基因组5 '端结合的宿主因子
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-22 DOI: 10.2217/fvl-2023-0070
Bader Y. Alhatlani, Waleed Aljabr, F. Alhamlan, A. Almatroudi, M. Azam, M. Alsaleem, K. Allemailem
Aim: The aim of this study was to identify host factors that interact with the 5′ end of the MERS-CoV RNA genome. Materials & methods: RNA affinity chromatography followed by mass spectrometry analysis was used to identify the binding of host factors in Vero E6 cells. Results: A total of 59 host factors that bound the MERS-CoV RNA genome in non-infected Vero E6 cells were identified. Most of the identified cellular proteins were previously reported to interact with the genome of other RNA viruses. We validated our mass spectrometry results using western blotting. Conclusion: These data enhance our knowledge about the RNA–host interactions of coronaviruses, which could serve as targets for developing antiviral therapeutics against MERS-CoV.
目的：本研究的目的是鉴定与MERS-CoV RNA基因组5′端相互作用的宿主因子。材料与方法：采用RNA亲和色谱法和质谱分析法对Vero E6细胞中宿主因子的结合进行鉴定。结果：在未感染的Vero E6细胞中，共鉴定出59个与MERS-CoV RNA基因组结合的宿主因子。大多数已鉴定的细胞蛋白先前被报道与其他RNA病毒的基因组相互作用。我们使用蛋白质印迹法验证了我们的质谱结果。结论：这些数据增强了我们对冠状病毒的RNA-宿主相互作用的了解，可以作为开发针对MERS-CoV的抗病毒疗法的靶点。
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引用次数: 0
Short hairpin RNA-mediated matrix gene silencing of human respiratory syncytial virus as a potent antiviral strategy 短发夹RNA介导的人呼吸道合胞病毒基质基因沉默作为一种有效的抗病毒策略
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-16 DOI: 10.2217/fvl-2022-0207
Saeid Amiri Zadeh Fard, Haniyeh Abuei, A. Farhadi, Gholamreza Rafiei Dehbidi, F. Zare, Zahra Abbasfard, A. Behbahani
Aim: Human respiratory syncytial virus (HRSV) is a common cause of respiratory infections, particularly in infants and the elderly. Ribavirin is the only US FDA-approved antiviral drug for HRSV infection, but it has unwanted side effects. Methods: We engineered an shRNA targeting the HRSV- M gene to antagonize HRSV replication. Results: The results showed that shRNA had a similarly significant reduction in viral load (99.8%) as ribavirin. In addition, combined treatment with ribavirin and M-shRNA resulted in a significant reduction in viral load compared with ribavirin or M-shRNA alone. Conclusion: These results suggest that M-shRNA could be a potential new inhibitor of HRSV replication and could offer a safer and more effective treatment option for HRSV infection in the future.
目的:人呼吸道合胞病毒(HRSV)是呼吸道感染的常见原因，特别是在婴儿和老年人中。利巴韦林是美国fda批准的唯一一种治疗HRSV感染的抗病毒药物，但是它有副作用。方法:我们设计了一种靶向HRSV- M基因的shRNA来对抗HRSV的复制。结果:结果表明shRNA与利巴韦林具有相似的显著的病毒载量降低(99.8%)。此外，与单独使用利巴韦林或M-shRNA相比，利巴韦林和M-shRNA联合治疗可显著降低病毒载量。结论:这些结果提示M-shRNA可能是一种潜在的新的HRSV复制抑制剂，并可能在未来为HRSV感染提供更安全、更有效的治疗选择。
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引用次数: 0
Kuwanon X from mulberry leaves exhibits antiviral activity against human adenoviruses 桑叶Kuwanon X对人腺病毒具有抗病毒活性
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-12 DOI: 10.2217/fvl-2023-0001
Chi Li, Jikui Deng, Lifeng Qi
Aim: This study aimed to investigate the anti–adenoviral activity of stilbene derivatives from mulberry leaves. Materials & methods: The anti–adenoviral activity was tested against adenoviruses -3 and -7 on human airway epithelial cell models. Cytotoxicity was assessed by LDH assay. Adenoviral DNA was quantified by qPCR. Results: All five tested stilbene derivatives from mulberry leaves exhibited anti–adenoviral activity, with Kuwanon X showing the highest inhibitory effect. Kuwanon X showed no apparent cytotoxicity for a wide range of concentrations. The mechanistic study revealed that Kuwanon X did not affect viral entry and nuclear translocation of the adenoviral genome but reduced viral DNA production. Conclusion: Stilbene derivatives like Kuwanon X from mulberry leaves are good candidates for antiviral treatment against AdV.
目的：研究桑叶二苯乙烯衍生物的抗腺病毒活性。材料与方法：在人气道上皮细胞模型上检测腺病毒-3和-7的抗腺病毒活性。细胞毒性通过LDH测定进行评估。腺病毒DNA通过qPCR进行定量。结果：5种桑叶二苯乙烯衍生物均具有抗腺病毒活性，其中Kuwanon X的抑制作用最强。Kuwanon X在大浓度范围内没有表现出明显的细胞毒性。机制研究表明，Kuwanon X不会影响病毒进入和腺病毒基因组的核转位，但会减少病毒DNA的产生。结论：桑叶二苯乙烯类化合物库瓦农X是治疗腺病毒的良好候选药物。
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引用次数: 0
Role of oxidative stress in the Epstein–Barr virus lifecycle and tumorigenicity 氧化应激在EB病毒生命周期和致瘤性中的作用
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-01 DOI: 10.2217/fvl-2023-0007
Zixiu Zhao, Wen Liu, B. Luo
The Epstein–Barr virus (EBV) is an oncogenic virus with both latent and lytic states during its lifecycle. EBV employs a latency period as a strategy to avoid host immune surveillance and achieve lifelong persistent infection. However, the latent state may be interrupted and EBV may reactivate into a lytic replication cycle, exacerbating transmission and pathogenicity. The balance and transition between these two phases in the EBV lifecycle are complex, and reactive oxygen species play an important role. We reviewed the relationship between oxidative stress and lytic replication of EBV, and the role of oxidative stress in the development of EBV-related tumors. Further research is required to explore and develop tumor antioxidant therapy.
EB病毒是一种致癌病毒，在其生命周期中具有潜伏状态和裂解状态。EBV采用潜伏期作为策略来避免宿主免疫监测并实现终身持续感染。然而，潜伏状态可能被中断，EBV可能重新激活进入裂解复制周期，加剧传播和致病性。EBV生命周期中这两个阶段之间的平衡和过渡是复杂的，活性氧物种发挥着重要作用。我们综述了氧化应激与EB病毒裂解复制之间的关系，以及氧化应激在EB病毒相关肿瘤发展中的作用。需要进一步的研究来探索和开发肿瘤抗氧化疗法。
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引用次数: 1
Plant protease inhibitors against SARS-CoV-2 main protease: an in silico approach 抗严重急性呼吸系统综合征冠状病毒2型主要蛋白酶的植物蛋白酶抑制剂：一种计算机方法
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-01 DOI: 10.2217/fvl-2022-0189
Adrianne M Lima, A. Á. de Souza, Jackson L. Amaral, V. N. Freire, Pedro F N Souza, H. D. de Oliveira
Aim: To evaluate using bioinformatics tools the interactions between plant protease inhibitors (PIs) and SARS-CoV-2 Mpro. Materials & methods: This was an in silico study based on molecular docking, molecular dynamics simulations and quantum biochemistry calculations. Results: The plant PIs pineapple cystatin and sesame cystatin interacted allosterically with Mpro, leading to significant structural alterations. These conformational changes lead to a reduction of the area and volume of the Mpro proteolytic site, likely affecting the protease activity and, consequently, reducing viral replication. Conclusion: This work highlights the therapeutic potential of plant PIs as candidates for future in vivo investigations into new therapeutics for COVID-19.
目的:利用生物信息学工具评价植物蛋白酶抑制剂(PIs)与SARS-CoV-2 Mpro的相互作用。材料与方法:这是一项基于分子对接、分子动力学模拟和量子生物化学计算的计算机研究。结果:菠萝胱抑素和芝麻胱抑素与Mpro发生变构相互作用，导致显著的结构改变。这些构象变化导致Mpro蛋白水解位点的面积和体积减少，可能影响蛋白酶活性，从而减少病毒复制。结论:本研究突出了植物pi作为未来COVID-19新疗法体内研究候选药物的治疗潜力。
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引用次数: 0
COVID-19 post vaccination neuronal adverse events: probable mechanisms and treatment possibilities COVID-19疫苗接种后神经元不良事件:可能的机制和治疗可能性
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-01 DOI: 10.2217/fvl-2023-0042
A. Baig, Joachim Gerlach, Prakash Salunke, Rachel Jessey, Robin Rose
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引用次数: 0
Cs-GRP78 recognition site on dengue virus envelope protein: in silico perspective 登革病毒包膜蛋白Cs-GRP78识别位点的计算机分析
4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-01 DOI: 10.2217/fvl-2022-0192
Abdo A Elfiky, Ahmed Amr, Amira Mosaad, Ahmed K Mubarak, Mohamed A Sayed, Kholoud K El-Halwany
Aim: To understand the binding of the dengue virus (DENV) envelope and the host cell factor, GRP78. Materials & methods: In this study, we simulate the binding of the DENV envelope against GRP78 using structural bioinformatics tools. Results: The sequence similarity of the DENV envelope C3–C30 and C302–C333 regions against the Pep42 cyclic peptide suggest these regions are possible recognition sites for GRP78. C3–C30 has a more similar grand average hydrophobicity index to that of Pep42 and a more negative binding affinity toward GRP78. Conclusion: We predict this region (C3–C30) of the DENV envelope to be the recognition site of GRP78. Further experimental validation will be important to future studies.
目的:了解登革病毒(DENV)包膜与宿主细胞因子GRP78的结合。材料,方法:在本研究中，我们使用结构生物信息学工具模拟DENV包膜与GRP78的结合。结果:DENV包膜C3-C30和C302-C333区域与Pep42环肽序列相似，提示这些区域可能是GRP78的识别位点。C3-C30的大平均疏水性指数与Pep42相似，对GRP78的结合亲和力为负。结论:我们预测DENV包膜的C3-C30区域是GRP78的识别位点。进一步的实验验证对未来的研究很重要。
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引用次数: 0
The role of quasi-enveloped hepatitis A virus in hepatitis A virus infection 准包膜甲型肝炎病毒在甲型肝炎病毒感染中的作用
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-01 DOI: 10.2217/fvl-2023-0046
Yuxue Zhao, Yiwen Chen, Qiaoqiao Chen, Chenxuan Bao, Huayuan Xiang, Lingxiang Mao
Hepatitis A virus (HAV) infection affects the global population and is responsible for acute hepatitis. Although most acute HAV infections can resolve spontaneously, there are about 15,000–30,000 deaths occurring annually worldwide. Therefore, it is important to study the mechanism of HAV infection. Recent studies have shown that HAV can be cloaked in the host membrane and exit cells nonlytically. This unique form of HAV is called quasi-enveloped HAV (eHAV) and is infectious and resistant to neutralizing antibodies. eHAV makes HAV different from many picornaviruses and provides a new pathway to HAV infection. In this review, we briefly summarize the characteristics and functions of eHAV in HAV infection.
甲型肝炎病毒(HAV)感染影响全球人口，并导致急性肝炎。虽然大多数急性甲肝感染可自行消退，但全世界每年仍有大约1.5万至3万人死亡。因此，研究HAV感染的机制具有重要意义。最近的研究表明，甲肝病毒可以隐藏在宿主膜内，非裂解性地离开细胞。这种独特形式的甲肝病毒被称为准包膜甲肝病毒(eHAV)，具有传染性，对中和抗体有抵抗力。eHAV使HAV不同于许多小核糖核酸病毒，为HAV感染提供了新的途径。本文就eHAV在HAV感染中的特点和作用作一综述。
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引用次数: 0
Structure-based virtual identification of natural inhibitors of SARS-CoV-2 and its Delta and Omicron variant proteins. 基于结构的SARS-CoV-2天然抑制剂及其Delta和Omicron变体蛋白的虚拟鉴定
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-01 Epub Date: 2023-06-01 DOI: 10.2217/fvl-2022-0184
Abdullah R Alanzi, Mohammad K Parvez, Mohammed S Al-Dosari

Aim: Structure-based identification of natural compounds against SARS-CoV-2, Delta and Omicron target proteins.

Materials & methods: Several known antiviral natural compounds were subjected to molecular docking and MD simulation against SARS-CoV-2 Mpro, Helicase and Spike, including Delta and Omicron Spikes.

Results: Of the docked ligands, 20 selected for each complex exhibited overall good binding affinities (-7.79 to -5.06 kcal/mol) with acceptable physiochemistry following Lipinski's rule. Finally, two best ligands from each complex upon simulation showed structural stability and compactness.

Conclusion: Quercetin-3-acetyl-glucoside, Rutin, Kaempferol, Catechin, Orientin, Obetrioside and Neridienone A were identified as potential inhibitors of SARS-CoV-2 Mpro, Helicase and Spike, while Orientin and Obetrioside also showed good binding affinities with Omicron Spike. Catechin and Neridienone A formed stable complexes with Delta Spike.

目的：基于结构鉴定抗严重急性呼吸系统综合征冠状病毒2型、德尔塔和奥密克戎靶蛋白的天然化合物。材料和方法：对几种已知的抗病毒天然化合物进行分子对接和MD模拟，以对抗严重急性呼吸系统综合征冠状病毒2型Mpro、螺旋酶和Spike，包括德尔塔和奥密克戎Spikes。结果：在对接的配体中，为每个复合物选择的20个显示出总体良好的结合亲和力（-7.79至-5.06 kcal/mol），具有可接受的物理化学性质，遵循利平斯基规则。最后，通过模拟，每个配合物中的两个最佳配体显示出结构稳定性和紧密性。结论：槲皮素-3-乙酰葡糖苷、芦丁、山奈酚、儿茶素、Orientin、Obetrioside和Neridienone A被鉴定为严重急性呼吸系统综合征冠状病毒2型Mpro、螺旋酶和Spike的潜在抑制剂，而Orientin和Obetrioide也显示出与奥密克戎Spike的良好结合亲和力。儿茶素和Neridienone A与三角穗形成稳定的复合物。
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引用次数: 1
CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice. CpG 684:小鼠COVID-19灭活疫苗的有效佐剂
IF 3.1 4区 医学 Q3 Immunology and Microbiology
Future Virology
Pub Date : 2023-05-01 Epub Date: 2023-06-01 DOI: 10.2217/fvl-2022-0172
Jiandong Liu, Tianle Cang, Congli Jiang, Kelei Li, Siyuan Liu, Haixin Wang, Meirong Wang, Yan Chen, Yan Shao, Jiankai Liu

Aim: This study used CpG 684 as adjuvant of inactivated COVID-19 vaccine to detect a humoral and cellular immune response in mice.

Materials & methods: We used 10 and 20 µg CpG 684 as adjuvants of an inactivated COVID-19 vaccine to immunize mice. IgG, IgG1, IgG2a, IgG2b and IgM binding antibodies were detected in serum by ELISA. The IFN-γ cytokine was detected by ELISPOT.

Results: CpG 684 improved spike-specific IgG and IgM subtype binding antibodies and increased the neutralizing antibody titer against prototype, Delta and Beta strains. CpG 684 also improved cellular immune response.

Conclusion: CpG 684 is an effective adjuvant for inactivated COVID-19 vaccine.

目的：应用CpG 684作为新冠肺炎灭活疫苗的佐剂，检测小鼠的体液和细胞免疫反应。材料与方法：我们使用10和20µg CpG 684作为新冠肺炎灭活疫苗的佐剂免疫小鼠。ELISA法检测血清中IgG、IgG1、IgG2a、IgG2b和IgM结合抗体。ELISPOT检测IFN-γ细胞因子。结果：CpG 684改善了刺突特异性IgG和IgM亚型结合抗体，并提高了针对原型、德尔塔和贝塔毒株的中和抗体滴度。CpG 684还改善了细胞免疫反应。结论：CpG 684是新冠肺炎灭活疫苗的有效佐剂。
{"title":"CpG 684: an effective adjuvant for the inactivated COVID-19 vaccine in mice.","authors":"Jiandong Liu, Tianle Cang, Congli Jiang, Kelei Li, Siyuan Liu, Haixin Wang, Meirong Wang, Yan Chen, Yan Shao, Jiankai Liu","doi":"10.2217/fvl-2022-0172","DOIUrl":"10.2217/fvl-2022-0172","url":null,"abstract":"<p><strong>Aim: </strong>This study used CpG 684 as adjuvant of inactivated COVID-19 vaccine to detect a humoral and cellular immune response in mice.</p><p><strong>Materials & methods: </strong>We used 10 and 20 µg CpG 684 as adjuvants of an inactivated COVID-19 vaccine to immunize mice. IgG, IgG1, IgG2a, IgG2b and IgM binding antibodies were detected in serum by ELISA. The IFN-γ cytokine was detected by ELISPOT.</p><p><strong>Results: </strong>CpG 684 improved spike-specific IgG and IgM subtype binding antibodies and increased the neutralizing antibody titer against prototype, Delta and Beta strains. CpG 684 also improved cellular immune response.</p><p><strong>Conclusion: </strong>CpG 684 is an effective adjuvant for inactivated COVID-19 vaccine.</p>","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48756097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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