Pub Date : 2023-10-01Epub Date: 2023-11-01DOI: 10.2217/fvl-2023-0115
Arantxa Horga, Rebecca Saenz, Gürdal Yilmaz, Abraham Simón-Campos, Keith Pietropaolo, William J Stubbings, Neil Collinson, Laura Ishak, Barbara Zrinscak, Bruce Belanger, Catherine Granier, Kai Lin, Aeron C Hurt, Xiao-Jian Zhou, Steffen Wildum, Janet Hammond
Aim: This phase III study assessed the efficacy/safety/antiviral activity/pharmacokinetics of bemnifosbuvir, a novel, oral nucleotide analog to treat COVID-19. Patients & methods: Outpatient adults/adolescents with mild-to-moderate COVID-19 were randomized 2:1 to bemnifosbuvir/placebo. Time to symptom alleviation/improvement (primary outcome), risk of hospitalization/death, viral load and safety were evaluated. Results: Although the study was discontinued prematurely and did not meet its primary end point, bemnifosbuvir treatment resulted in fewer hospitalizations (71% relative risk reduction), COVID-19-related medically attended hospital visits, and COVID-19-related complications compared with placebo. No reduction in viral load was observed. The proportion of patients with adverse events was similar; no deaths occurred. Conclusion: Bemnifosbuvir showed hospitalization reduction in patients with variable disease progression risk and was well tolerated. Clinical Trial Registration: NCT04889040 (ClinicalTrials.gov).
{"title":"Oral bemnifosbuvir (AT-527) vs placebo in patients with mild-to-moderate COVID-19 in an outpatient setting (MORNINGSKY).","authors":"Arantxa Horga, Rebecca Saenz, Gürdal Yilmaz, Abraham Simón-Campos, Keith Pietropaolo, William J Stubbings, Neil Collinson, Laura Ishak, Barbara Zrinscak, Bruce Belanger, Catherine Granier, Kai Lin, Aeron C Hurt, Xiao-Jian Zhou, Steffen Wildum, Janet Hammond","doi":"10.2217/fvl-2023-0115","DOIUrl":"10.2217/fvl-2023-0115","url":null,"abstract":"<p><p><b>Aim:</b> This phase III study assessed the efficacy/safety/antiviral activity/pharmacokinetics of bemnifosbuvir, a novel, oral nucleotide analog to treat COVID-19. <b>Patients & methods:</b> Outpatient adults/adolescents with mild-to-moderate COVID-19 were randomized 2:1 to bemnifosbuvir/placebo. Time to symptom alleviation/improvement (primary outcome), risk of hospitalization/death, viral load and safety were evaluated. <b>Results:</b> Although the study was discontinued prematurely and did not meet its primary end point, bemnifosbuvir treatment resulted in fewer hospitalizations (71% relative risk reduction), COVID-19-related medically attended hospital visits, and COVID-19-related complications compared with placebo. No reduction in viral load was observed. The proportion of patients with adverse events was similar; no deaths occurred. <b>Conclusion:</b> Bemnifosbuvir showed hospitalization reduction in patients with variable disease progression risk and was well tolerated. <b>Clinical Trial Registration</b>: NCT04889040 (ClinicalTrials.gov).</p>","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71480363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01Epub Date: 2023-11-07DOI: 10.2217/fvl-2023-0036
Shahd A Mohammedain, Saif Badran, AbdelNaser Y Elzouki, Halla Salim, Ayesha Chalaby, Mya Siddiqui, Yehia Y Hussein, Hanan Abdul Rahim, Lukman Thalib, Mohammed Fasihul Alam, Daoud Al-Badriyeh, Sumaya Al-Maadeed, Suhail Ar Doi
Aim: This study aims to perform an external validation of a recently developed prognostic model for early prediction of the risk of progression to severe COVID-19. Patients & methods/materials: Patients were recruited at their initial diagnosis at two facilities within Hamad Medical Corporation in Qatar. 356 adults were included for analysis. Predictors for progression of COVID-19 were all measured at disease onset and first contact with the health system. Results: The C statistic was 83% (95% CI: 78%-87%) and the calibration plot showed that the model was well-calibrated. Conclusion: The published prognostic model for the progression of COVID-19 infection showed satisfactory discrimination and calibration and the model is easy to apply in clinical practice.d.
{"title":"Validation of a risk prediction model for COVID-19: the PERIL prospective cohort study.","authors":"Shahd A Mohammedain, Saif Badran, AbdelNaser Y Elzouki, Halla Salim, Ayesha Chalaby, Mya Siddiqui, Yehia Y Hussein, Hanan Abdul Rahim, Lukman Thalib, Mohammed Fasihul Alam, Daoud Al-Badriyeh, Sumaya Al-Maadeed, Suhail Ar Doi","doi":"10.2217/fvl-2023-0036","DOIUrl":"https://doi.org/10.2217/fvl-2023-0036","url":null,"abstract":"<p><p><b>Aim:</b> This study aims to perform an external validation of a recently developed prognostic model for early prediction of the risk of progression to severe COVID-19. <b>Patients & methods/materials:</b> Patients were recruited at their initial diagnosis at two facilities within Hamad Medical Corporation in Qatar. 356 adults were included for analysis. Predictors for progression of COVID-19 were all measured at disease onset and first contact with the health system. <b>Results:</b> The C statistic was 83% (95% CI: 78%-87%) and the calibration plot showed that the model was well-calibrated. <b>Conclusion:</b> The published prognostic model for the progression of COVID-19 infection showed satisfactory discrimination and calibration and the model is easy to apply in clinical practice.d.</p>","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zeinab Vosough, Farzin Sadeghi, G. Kamrani, A. Hasanzadeh, Mohammad Ranaee
Aim: We aimed to investigate the presence of human papillomavirus type 197 (HPV197) in patients with cutaneous squamous cell carcinoma (SCC) and basal cell carcinoma (BCC). Materials & methods: 58 formalin-fixed paraffin-embedded tissue samples, including 12 SCC and 46 BCC and the adjacent non-tumoral skin of the same patient were analyzed for the presence of HPV197E6 gene, using a quantitative real-time PCR assay. Results: The HPV197E6 gene was identified in 12 tumor samples (7 BCC and 5 SCC), but not in the control group. Conclusion: Based on these results, a high copy number of HPV197E6 gene provides additional support for the role of HPV197 in skin cancer.
{"title":"Evaluation of human papillomavirus type 197 genome in non-melanoma skin cancer and adjacent non-tumoral skin margins","authors":"Zeinab Vosough, Farzin Sadeghi, G. Kamrani, A. Hasanzadeh, Mohammad Ranaee","doi":"10.2217/fvl-2023-0022","DOIUrl":"https://doi.org/10.2217/fvl-2023-0022","url":null,"abstract":"Aim: We aimed to investigate the presence of human papillomavirus type 197 (HPV197) in patients with cutaneous squamous cell carcinoma (SCC) and basal cell carcinoma (BCC). Materials & methods: 58 formalin-fixed paraffin-embedded tissue samples, including 12 SCC and 46 BCC and the adjacent non-tumoral skin of the same patient were analyzed for the presence of HPV197E6 gene, using a quantitative real-time PCR assay. Results: The HPV197E6 gene was identified in 12 tumor samples (7 BCC and 5 SCC), but not in the control group. Conclusion: Based on these results, a high copy number of HPV197E6 gene provides additional support for the role of HPV197 in skin cancer.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47928648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Azimi, Sina Nasrollahian, S. Sabour, Nahal Hadi, L. Azimi, N. Rahbarian, A. Karimi, F. Fallah, Roxana Mansour-Ghanaie, S. Hoseini-Alfatemi, S. A. Fahimzad
Aim: The present study aimed to investigate the frequency of Seoul hantavirus (SEOV), Hepatitis E and rabies viruses in Norway rats in Tehran, Iran. Methods: With the aid of a rat ELISA kit, the we identified specific IgG antibodies against the Rabies virus. The presence of SEOV and HEV was determined using an SYBR Green-based real-time PCR assay. Results: 1% of the R. norvegicus carried the rabies virus. HEV was associated with R. norvegicus obtained from the south of Tehran with the highest frequency (35%). 12% of tests for SEOV were positive. Conclusion: The findings highlight the importance of putting in place rodent control programs and avoiding interaction with rodent populations in urban settings.
{"title":"The first evidence of Seoul hantavirus, hepatitis E virus and rabies virus in Rattus norvegicus in Tehran, Iran","authors":"T. Azimi, Sina Nasrollahian, S. Sabour, Nahal Hadi, L. Azimi, N. Rahbarian, A. Karimi, F. Fallah, Roxana Mansour-Ghanaie, S. Hoseini-Alfatemi, S. A. Fahimzad","doi":"10.2217/fvl-2023-0047","DOIUrl":"https://doi.org/10.2217/fvl-2023-0047","url":null,"abstract":"Aim: The present study aimed to investigate the frequency of Seoul hantavirus (SEOV), Hepatitis E and rabies viruses in Norway rats in Tehran, Iran. Methods: With the aid of a rat ELISA kit, the we identified specific IgG antibodies against the Rabies virus. The presence of SEOV and HEV was determined using an SYBR Green-based real-time PCR assay. Results: 1% of the R. norvegicus carried the rabies virus. HEV was associated with R. norvegicus obtained from the south of Tehran with the highest frequency (35%). 12% of tests for SEOV were positive. Conclusion: The findings highlight the importance of putting in place rodent control programs and avoiding interaction with rodent populations in urban settings.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42547487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bisma Rauff, Mehdi Ramezani-Moghadam, Enoch S. E. Tay, Jacob George, M. Douglas
Aim: To investigate the mechanism of liver fibrosis in chronic hepatitis C virus (HCV) infection we developed an in vitro model. Methods: Huh7 cells were transfected with HCV (JFH1 or Jc1) or sub-genomic replicons and expression of pro-fibrotic cytokines determined by qPCR. Media from infected cells was transferred onto LX2 cells or primary rat stellate cells and expression of pro-fibrotic genes measured by qPCR. Results: Replication competent HCV, but not sub-genomic replicons, induced expression of TGF-β1 and CTGF. Supernatant from infected cells induced α-SMA and COL1A1 expression and stellate cell migration, confirming functional activation. Conclusion: Infected cells produce pro-fibrogenic cytokines TGF-β1 and CTGF and activate stellate cells. This mechanism could be targeted to prevent or treat HCV-induced fibrosis.
{"title":"Development of an in vitro model to study hepatitis C virus-induced fibrosis","authors":"Bisma Rauff, Mehdi Ramezani-Moghadam, Enoch S. E. Tay, Jacob George, M. Douglas","doi":"10.2217/fvl-2022-0206","DOIUrl":"https://doi.org/10.2217/fvl-2022-0206","url":null,"abstract":"Aim: To investigate the mechanism of liver fibrosis in chronic hepatitis C virus (HCV) infection we developed an in vitro model. Methods: Huh7 cells were transfected with HCV (JFH1 or Jc1) or sub-genomic replicons and expression of pro-fibrotic cytokines determined by qPCR. Media from infected cells was transferred onto LX2 cells or primary rat stellate cells and expression of pro-fibrotic genes measured by qPCR. Results: Replication competent HCV, but not sub-genomic replicons, induced expression of TGF-β1 and CTGF. Supernatant from infected cells induced α-SMA and COL1A1 expression and stellate cell migration, confirming functional activation. Conclusion: Infected cells produce pro-fibrogenic cytokines TGF-β1 and CTGF and activate stellate cells. This mechanism could be targeted to prevent or treat HCV-induced fibrosis.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":"20 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68215671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Future VirologyAhead of Print EditorialYellow fever: is Asia bound to encounter the virus?Baijayantimala Mishra, Sutapa Rath, Monalisa Mohanty & Prabhudutta MamidiBaijayantimala Mishra *Author for correspondence: Tel.: +91 943 888 4121; E-mail Address: bmishramicro@gmail.comhttps://orcid.org/0000-0002-2604-6678Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author, Sutapa Rath https://orcid.org/0000-0002-0790-1448Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author, Monalisa Mohanty https://orcid.org/0000-0003-3940-1998Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author & Prabhudutta Mamidi https://orcid.org/0000-0002-0187-571XDepartment of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this authorPublished Online:8 Sep 2023https://doi.org/10.2217/fvl-2023-0128AboutSectionsView ArticleView Full TextPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInRedditEmail View articleKeywords: aedes mosquitoAfricaarbovirusAsiatransmissionyellow feverPapers of special note have been highlighted as: • of interest; •• of considerable interestReferences1. Kallas EG, Wilder-Smith A. Managing severe yellow fever in the intensive care: lessons learnt from Brazil. J. Travel Med. 26(5), taz043 (2019). • Paper that describes the clinical spectrums of yellow fever.Crossref, Medline, Google Scholar2. Ho YL, Joelsons D, Leite GFC et al. Severe yellow fever in Brazil: clinical characteristics and management. J. Travel Med. 26(5), taz040 (2019). • A study that described the clinical management of severe yellow fever infection.Crossref, Medline, Google Scholar3. Cunha MDP, Duarte-Neto AN, Pour SZ et al. Phylogeographic patterns of the yellow fever virus around the metropolitan region of São Paulo, Brazil, 2016–2019. PLOS Negl. Trop. Dis. 16(9), e0010705 (2022).Crossref, Medline, CAS, Google Scholar4. Rodríguez-Morales AJ, Bonilla-Aldana DK, Suárez JA et al. Yellow fever reemergence in Venezuela - Implications for international travellers and Latin American countries during the COVID-19 pandemic. Travel Med. Infect. Dis. 44, 102192 (2021). • Article on importance of immunization coverage and optimization of surveillance system for populations at risk of yellow fever.Crossref, Medline, CAS, Google Scholar5. Gardner CL, Ryman KD. Yellow fever: a reemerging threat. Clin. Lab. Med. 30(1), 237–260 (2010).Crossref, Medline, Google Scholar6. Gubler DJ. Pandemic yellow fever: a potential threat to global health via travellers. J. Travel Med. 25(1), (2018). •• One of the earliest paper emphasising on the pandemic potential of yellow fever.Crossref, Medline, Google Scholar7. Song R, Guan S, Le
未来病毒学:黄热病:亚洲一定会遇到这种病毒吗?Baijayantimala Mishra, Sutapa Rath, Monalisa Mohanty & Prabhudutta MamidiBaijayantimala Mishra *通讯作者:电话:+91 943 888 4121;电子邮件地址:bmishramicro@gmail.comhttps://orcid.org/0000-0002-2604-6678Department，全印度医学科学研究所，布巴内斯瓦尔，751019，印度奥里萨邦。搜索更多作者的论文，Sutapa Rath https://orcid.org/0000-0002-0790-1448Department，全印度医学科学研究所，布巴内斯瓦尔，751019，印度奥里萨邦。搜索更多作者的论文，Monalisa Mohanty https://orcid.org/0000-0003-3940-1998Department，微生物学。搜索本作者和Prabhudutta Mamidi的更多论文https://orcid.org/0000-0002-0187-571XDepartment，全印度医学科学研究所，布巴内斯瓦尔，751019，奥里萨邦，印度搜索本文作者的更多论文发表在线:2023年9月8日https://doi.org/10.2217/fvl-2023-0128AboutSectionsView文章查看全文pdf /EPUB工具添加到收藏下载CitationsTrack CitationsPermissionsReprints转载分享分享onFacebookTwitterLinkedInRedditEmail查看文章关键词:伊蚊非洲病毒亚洲传播黄热病特别注意的论文已被突出显示为:•感兴趣;有相当大的兴趣参考资料Kallas EG, Wilder-Smith A.重症监护中的重症黄热病管理:从巴西吸取的经验教训。[j] .中华旅游杂志，2016,33(5):559 - 567。•描述黄热病临床谱的论文。Crossref, Medline, Google Scholar2。何玉林，赵志强，李志强，等。巴西严重黄热病:临床特征和管理。[j] .旅游医学，26(5)，39(2019)。•一项描述严重黄热病感染临床管理的研究。Crossref, Medline, Google Scholar3。Cunha MDP, Duarte-Neto AN, Pour SZ等。2016-2019年巴西<s:1>圣保罗大都会区黄热病病毒的系统地理格局公共科学图书馆Negl。太。第16(9)，e0010705(2022)。Crossref, Medline, CAS, Google Scholar4。Rodríguez-Morales AJ, Bonilla-Aldana DK, Suárez JA等。黄热病在委内瑞拉再次出现- COVID-19大流行期间对国际旅行者和拉丁美洲国家的影响。旅行医学。感染。第44卷，102192(2021)。•关于免疫覆盖和优化黄热病高危人群监测系统的重要性的文章。Crossref, Medline, CAS, Google Scholar5。加德纳CL，莱曼KD。黄热病:再次出现的威胁。中国。实验室。医学杂志，30(1)，237-260(2010)。Crossref, Medline, Google Scholar6。Gubler DJ。大流行性黄热病:通过旅行者对全球健康的潜在威胁。旅游医学，25(1)，(2018)。••最早强调黄热病大流行潜力的论文之一。Crossref, Medline, Google Scholar7。宋荣，关生，李世生等。黄热病从安哥拉传入中国与疫苗接种延迟或缺乏有关。紧急情况。感染。病案24(7)，1383-1386(2018)。Crossref, Medline, CAS, Google Scholar8。联合国儿童基金会。在安哥拉将黄热病病例保持在零。https://www.unicef.org/stories/angola-keeping-yellow-fever-cases-zero•描述和更新安哥拉目前的黄热病情况。谷歌Scholar9。黄热病在亚洲的缺席:历史、假设、媒介传播、亚洲黄热病的可能性和其他谜团。病毒。12(12)，1349(2020)。Crossref, Medline, CAS, Google Scholar10。巴拿马运河与黄热病传入亚洲的关系。Tran。论文。Soc。洛德，22,60-100(1903)。•最早描述黄热病从流行地区传播到亚洲可能模式的文章之一。Medline, Google Scholar11。Baker RE, Mahmud AS, Miller IF等。全球变化时代的传染病。微生物学通报，2011(4)，393 - 395(2022)。••审查气候变化和其他关键决定因素对新出现和重新出现的传染病的影响。Crossref, Medline, CAS, Google Scholar12。Brent SE, Watts A, Cetron M等。易受黄热病传播影响的全球城市中心之间的国际旅行。世界卫生学报，96(5)，343-354B(2018)。Crossref, Medline, Google Scholar13。Blake LE, Garcia-Blanco MA。人类基因变异与19世纪美国黄热病流行期间的死亡率。mBio。5(3)， e01253-14(2014)。Crossref, Medline, Google Scholar14。Saron WAA, Rathore APS, Ting L等。黄病毒血清复合体交叉反应性免疫通过激活异源记忆CD4 T细胞起到保护作用。科学通报，4(7)，2018,44(6)。Crossref, Medline, Google Scholar15。Rosser JI, Nielsen-Saines K, Saad E, Fuller T. 2017-2018年巴西东南部黄热病病毒再次出现:是什么引发了传播?公共科学图书馆Negl。太。第16(2)，e0010133(2022)。
{"title":"Yellow fever: is Asia bound to encounter the virus?","authors":"Baijayantimala Mishra, Sutapa Rath, Monalisa Mohanty, Prabhudutta Mamidi","doi":"10.2217/fvl-2023-0128","DOIUrl":"https://doi.org/10.2217/fvl-2023-0128","url":null,"abstract":"Future VirologyAhead of Print EditorialYellow fever: is Asia bound to encounter the virus?Baijayantimala Mishra, Sutapa Rath, Monalisa Mohanty & Prabhudutta MamidiBaijayantimala Mishra *Author for correspondence: Tel.: +91 943 888 4121; E-mail Address: bmishramicro@gmail.comhttps://orcid.org/0000-0002-2604-6678Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author, Sutapa Rath https://orcid.org/0000-0002-0790-1448Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author, Monalisa Mohanty https://orcid.org/0000-0003-3940-1998Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this author & Prabhudutta Mamidi https://orcid.org/0000-0002-0187-571XDepartment of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, 751019, Odisha, IndiaSearch for more papers by this authorPublished Online:8 Sep 2023https://doi.org/10.2217/fvl-2023-0128AboutSectionsView ArticleView Full TextPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInRedditEmail View articleKeywords: aedes mosquitoAfricaarbovirusAsiatransmissionyellow feverPapers of special note have been highlighted as: • of interest; •• of considerable interestReferences1. Kallas EG, Wilder-Smith A. Managing severe yellow fever in the intensive care: lessons learnt from Brazil. J. Travel Med. 26(5), taz043 (2019). • Paper that describes the clinical spectrums of yellow fever.Crossref, Medline, Google Scholar2. Ho YL, Joelsons D, Leite GFC et al. Severe yellow fever in Brazil: clinical characteristics and management. J. Travel Med. 26(5), taz040 (2019). • A study that described the clinical management of severe yellow fever infection.Crossref, Medline, Google Scholar3. Cunha MDP, Duarte-Neto AN, Pour SZ et al. Phylogeographic patterns of the yellow fever virus around the metropolitan region of São Paulo, Brazil, 2016–2019. PLOS Negl. Trop. Dis. 16(9), e0010705 (2022).Crossref, Medline, CAS, Google Scholar4. Rodríguez-Morales AJ, Bonilla-Aldana DK, Suárez JA et al. Yellow fever reemergence in Venezuela - Implications for international travellers and Latin American countries during the COVID-19 pandemic. Travel Med. Infect. Dis. 44, 102192 (2021). • Article on importance of immunization coverage and optimization of surveillance system for populations at risk of yellow fever.Crossref, Medline, CAS, Google Scholar5. Gardner CL, Ryman KD. Yellow fever: a reemerging threat. Clin. Lab. Med. 30(1), 237–260 (2010).Crossref, Medline, Google Scholar6. Gubler DJ. Pandemic yellow fever: a potential threat to global health via travellers. J. Travel Med. 25(1), (2018). •• One of the earliest paper emphasising on the pandemic potential of yellow fever.Crossref, Medline, Google Scholar7. Song R, Guan S, Le","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136298523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Avipsha Sarkar, Anirban Roy, Madhulina Maity, D. Nayak, Satadal Das
Aim: This study aimed to alleviate dengue virus (DENV)-mediated fibrosis in a chick embryo model using ultradiluted Eupatorium perfoliatum (UEP). Materials & methods: Chorioallantoic membrane and liver of infected embryonated chicken eggs were checked for DENV pathogenesis. Cytotoxicity of UEP was assessed by MTT and scratch assay. Cytopathic assay after DENV infection was performed in vitro to check the effect of UEP and its constituents. Histopathology analysis revealed DENV-mediated fibrosis and the effect of UEP. qRT-PCR and gelatin zymography showed levels of fibrosis-causing genes. Results: UEP showed low cytotoxicity and maximum potential in mitigating DENV-mediated fibrosis in vivo via regulation of fibrosis biomarkers TGF-β, MMP-1, MMP-9, TIMP-1 and IFNs. Conclusion: UEP may be considered to alleviate DENV-mediated pathogenesis.
{"title":"Ultradiluted Eupatorium perfoliatum alleviates DENV-induced fibrosis by regulation of TGFβ1, MMP-9 and interferons","authors":"Avipsha Sarkar, Anirban Roy, Madhulina Maity, D. Nayak, Satadal Das","doi":"10.2217/fvl-2023-0121","DOIUrl":"https://doi.org/10.2217/fvl-2023-0121","url":null,"abstract":"Aim: This study aimed to alleviate dengue virus (DENV)-mediated fibrosis in a chick embryo model using ultradiluted Eupatorium perfoliatum (UEP). Materials & methods: Chorioallantoic membrane and liver of infected embryonated chicken eggs were checked for DENV pathogenesis. Cytotoxicity of UEP was assessed by MTT and scratch assay. Cytopathic assay after DENV infection was performed in vitro to check the effect of UEP and its constituents. Histopathology analysis revealed DENV-mediated fibrosis and the effect of UEP. qRT-PCR and gelatin zymography showed levels of fibrosis-causing genes. Results: UEP showed low cytotoxicity and maximum potential in mitigating DENV-mediated fibrosis in vivo via regulation of fibrosis biomarkers TGF-β, MMP-1, MMP-9, TIMP-1 and IFNs. Conclusion: UEP may be considered to alleviate DENV-mediated pathogenesis.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48930161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saeed Samadizadeh, Ali Vaez, Z. Jamalpoor, A. Tahamtan
Aim: Identifying genes and pathways involved in various stages of rotavirus infection could facilitate the mapping of the interactions between the virus and host. Materials & methods: Weighted gene co-expression network analysis (WGCNA) was employed to construct a co-expression network of genes identified by microarray analysis from samples from children with acute rotavirus infection. Results: Two specific modules were most related to rotavirus infection. The analysis of differentially expressed genes (DEGs) disclosed 393 DEGs between normal and infected individuals. Eighteen novel genes were shared between the modules. Conclusion: The WGCNA revealed novel modules, co-expressed genes and pathways involved in immune responses, cell death/degradation and the nervous system in acute rotavirus infection.
{"title":"Highlighting the role of immune responses, cell death and the nervous system in acute rotavirus infection using weighted gene co-expression network analysis","authors":"Saeed Samadizadeh, Ali Vaez, Z. Jamalpoor, A. Tahamtan","doi":"10.2217/fvl-2023-0045","DOIUrl":"https://doi.org/10.2217/fvl-2023-0045","url":null,"abstract":"Aim: Identifying genes and pathways involved in various stages of rotavirus infection could facilitate the mapping of the interactions between the virus and host. Materials & methods: Weighted gene co-expression network analysis (WGCNA) was employed to construct a co-expression network of genes identified by microarray analysis from samples from children with acute rotavirus infection. Results: Two specific modules were most related to rotavirus infection. The analysis of differentially expressed genes (DEGs) disclosed 393 DEGs between normal and infected individuals. Eighteen novel genes were shared between the modules. Conclusion: The WGCNA revealed novel modules, co-expressed genes and pathways involved in immune responses, cell death/degradation and the nervous system in acute rotavirus infection.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45271568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N6-methyladenosine (m6A) modification is the most pervasive type of RNA epigenetic modification in eukaryotes and the most common type of posttranscriptional modification of mRNA. m6A modification is a dynamic reversible process that can affect gene expression and play a crucial role in mRNA metabolism and multiple biological processes, ranging from RNA processing, nuclear export and RNA translation to decay. Meanwhile, much evidence has shown that m6A methylation regulates the life cycle of viruses and inhibits or promotes the development of various viruses. However, the mechanism of m6A methylation in virus-associated tumors has not been fully elucidated. This review highlights the role of m6A modification in various viruses to help establish new approaches for treating related diseases.
{"title":"N6-methyladenosine methylation of viral genes","authors":"Jiting Sun, Yan Zhang, Bin Luo","doi":"10.2217/fvl-2022-0201","DOIUrl":"https://doi.org/10.2217/fvl-2022-0201","url":null,"abstract":"N6-methyladenosine (m6A) modification is the most pervasive type of RNA epigenetic modification in eukaryotes and the most common type of posttranscriptional modification of mRNA. m6A modification is a dynamic reversible process that can affect gene expression and play a crucial role in mRNA metabolism and multiple biological processes, ranging from RNA processing, nuclear export and RNA translation to decay. Meanwhile, much evidence has shown that m6A methylation regulates the life cycle of viruses and inhibits or promotes the development of various viruses. However, the mechanism of m6A methylation in virus-associated tumors has not been fully elucidated. This review highlights the role of m6A modification in various viruses to help establish new approaches for treating related diseases.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43203831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junhong Luo, Yao Huang, Yan Guo, Zixinrong Meng, Kangchen Zhao, Xiaojuan Zhu, Le Zhou, Tao Wu, Qiao Qiao, Yiyue Ge, Qin Xu, Lunbiao Cui
Aim: This study aimed to establish a simple whole-genome sequencing method for respiratory virus type A (RSVA) used in genomic epidemiological studies. Methods: Multiple primers were designed to amplify overlapping amplicons specific to RSVA. The amplicons were sequenced using Illumina and Nanopore sequencing platforms and the sequencing performances of the two platforms were also compared. Results: The study shows that the developed method could recover almost full genomes of RSVA when Ct values were less than 33 for clinical samples. Conclusion: In this study, a whole-genome sequencing method was developed for RSVA based on multiplex PCR.
{"title":"Establishment and evaluation of a multiplex PCR amplification-based sequencing method for respiratory virus type A","authors":"Junhong Luo, Yao Huang, Yan Guo, Zixinrong Meng, Kangchen Zhao, Xiaojuan Zhu, Le Zhou, Tao Wu, Qiao Qiao, Yiyue Ge, Qin Xu, Lunbiao Cui","doi":"10.2217/fvl-2023-0083","DOIUrl":"https://doi.org/10.2217/fvl-2023-0083","url":null,"abstract":"Aim: This study aimed to establish a simple whole-genome sequencing method for respiratory virus type A (RSVA) used in genomic epidemiological studies. Methods: Multiple primers were designed to amplify overlapping amplicons specific to RSVA. The amplicons were sequenced using Illumina and Nanopore sequencing platforms and the sequencing performances of the two platforms were also compared. Results: The study shows that the developed method could recover almost full genomes of RSVA when Ct values were less than 33 for clinical samples. Conclusion: In this study, a whole-genome sequencing method was developed for RSVA based on multiplex PCR.","PeriodicalId":12505,"journal":{"name":"Future Virology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135346818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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