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Indicators of oxidative stress in blood samples of indigenous residents and newcomers in the Arctic zone of Yakutia 雅库特北极地区土著居民和新移民血液样本中的氧化应激指标
Q4 Medicine Pub Date : 2023-08-30 DOI: 10.47470/0016-9900-2023-102-7-624-631
L. Khripach, T. D. Knyazeva, Z. I. Koganova, Evgeniia V. Zheleznyak, Anzhelika V. Zagaynova
Introduction. Oxidative stress is non-specific reaction of human organism in response to various damaging factors, including climatic. The purpose of the study. To compare markers of oxidative stress and corresponding age dependences in blood samples of indigenous (evolutionarily adapted) and newcomer inhabitants of the Arctic zone of Yakutia. Materials and methods. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and malondialdehyde content (MDA) were determined in blood lysates of indigenous (n=100) and newcomers (n=37) residents of Chokurdakh and Tiksi settlements. The compared subsamples did not differ in age (medians 34 and 37 years, p=0.407). Results. No significant differences were found between newcomers and natives in terms of SOD, CAT and MDA content in the blood. The GPx activity of newcomers was 1.2 times higher than that of the natives (27.8 [22.4; 32.0] and 23.4 [19.2; 29.4] U/g Hb, p=0.042), but rapidly decreased with age (R= –0.549; p=0.001) in parallel with the increase in MDA content (R=0.420; p=0.01), whereas the indigenous people had no age-related changes in GPx and MDA. Limitations. Associated with a comparatively modest sample size (137 persons). Conclusion. According to modern gerontology, age-related trends in enzyme activity arise due to changes in regulation of corresponding genes and reflect the rate of aging of the population. So it can be assumed that our data, which show accelerated aging of Arctic alien inhabitants compared to the indigenous ones, can be explained by genetic polymorphism of GPx1 transcription factors.
介绍。氧化应激是人体机体对各种有害因素(包括气候)的非特异性反应。研究的目的。比较雅库特北极地区土著居民(进化适应)和新居民血液样本中的氧化应激标记物和相应的年龄依赖性。材料和方法。测定了100名Chokurdakh和Tiksi定居地土著居民(n=100)和新移民(n=37)血液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)的活性以及丙二醛(MDA)含量。比较的亚样本在年龄上没有差异(中位数为34岁和37岁,p=0.407)。结果。在血液中SOD、CAT和MDA的含量方面,新移民与当地居民无显著差异。新来者的GPx活性是本地人的1.2倍(27.8 [22.4;32.0]和23.4 [19.2;29.4] U/g Hb, p=0.042),但随年龄增长迅速下降(R= -0.549;p=0.001),且MDA含量升高(R=0.420;p=0.01),而土著居民GPx和MDA无年龄相关性变化。的局限性。与相对适度的样本量(137人)相关。结论。现代老年学认为,与年龄相关的酶活性变化趋势是由于相应基因调控的变化而产生的,反映了人口老龄化的速度。因此,我们可以假设,我们的数据显示,与本地居民相比,北极外来居民的衰老速度加快,可以用GPx1转录因子的遗传多态性来解释。
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引用次数: 1
Pathogenesis of morphological changes in organs and tissues in chronic fluoride intoxication (literature review) 慢性氟中毒脏器组织形态改变的发病机制(文献复习)
Q4 Medicine Pub Date : 2023-08-30 DOI: 10.47470/0016-9900-2023-102-7-700-705
M. S. Bugaeva, A. S. Kazitskaya, T. K. Yadykina, A. G. Zhukova, N. Mikhailova
Introduction. Traditionally, the main manifestation of chronic fluoride intoxication is the damage to the skeletal system, while during recent years a large amount of works has been accumulated on the study of the mechanism of action of both fluorine and its compounds on non-skeletal tissues. Based on the analysis of the Russian Science Citation Index, PubMed, Scopus, Web of Science, MedLine, Google Scholar, CyberLeninka databases, a review of contemporary concepts of morphological changes in organs and systems under the conditions of long-term exposure to fluorine compounds is presented. Effect of fluorine on the musculoskeletal system. Fluorine Ihas been shown to induce differentiation and apoptosis of osteoblasts and osteoclasts, leading to various skeletal injuries. Active osteogenesis and accelerated bone metabolism are key processes in the progression of skeletal fluorosis and the pathological base of the diversity of osteogenic lesions. Effect of fluorine on non-skeletal tissues. Data on the effect of fluorine on the induction of free radical oxidation in the cerebral cortex, redistribution, and damage to nerve cells are presented. Activation of apoptosis, oxidative stress, and a decrease in the activity of antioxidant enzymes under the influence of sodium fluoride in the heart muscle are shown, data on the development of functional and structural disorders in the myocardium are given. Due to fluoride intoxication, the formation of degenerative and inflammatory changes in the respiratory system, liver, and kidneys, structural disorders and dysfunction of the thyroid gland, as well as an increase in the level of reactive oxygen species and the induction of apoptosis in these organs are shown. Data on the effect of sodium fluoride on the histological structure of the reproductive organs with a decrease in sperm motility, lack of differentiation and maturation of spermatocytes, intensification of free radical oxidation and cell apoptosis are presented. Conclusion. The analysis carried out allows expanding the understanding of the pathogenetic bases of morphological changes and functional failure of organs and tissues under the conditions of chronic fluoride intoxication, to substantiate a systemic, integrated approach to its treatment and prevention.
介绍。传统上,慢性氟中毒的主要表现为骨骼系统的损伤,近年来,氟及其化合物对非骨骼组织的作用机制的研究已经积累了大量的工作。通过对俄罗斯科学引文索引、PubMed、Scopus、Web of Science、MedLine、谷歌Scholar、CyberLeninka等数据库的分析,综述了长期暴露于氟化合物条件下器官和系统形态变化的当代概念。氟对肌肉骨骼系统的影响。氟已被证明可诱导成骨细胞和破骨细胞分化和凋亡,导致各种骨骼损伤。活跃的成骨和加速的骨代谢是氟骨症进展的关键过程,也是成骨病变多样性的病理基础。氟对非骨骼组织的影响。关于氟对诱导大脑皮层自由基氧化、再分布和神经细胞损伤的影响的数据被提出。在氟化钠的影响下,心肌细胞凋亡的激活、氧化应激和抗氧化酶活性的降低被显示出来,并给出了心肌功能和结构紊乱发展的数据。氟中毒可导致呼吸系统、肝脏和肾脏出现退行性和炎症性改变,甲状腺结构紊乱和功能障碍,这些器官的活性氧水平升高并诱导细胞凋亡。本文介绍了氟化钠对生殖器官组织结构的影响,包括精子活力降低、精母细胞分化和成熟不足、自由基氧化加剧和细胞凋亡。结论。进行的分析可以扩大对慢性氟化物中毒条件下器官和组织形态变化和功能衰竭的发病基础的理解,为其治疗和预防提供系统、综合的方法。
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引用次数: 0
Effects of daily exposure to peat smoke on the reproductive and nervous systems in male white rats 泥炭烟对雄性大鼠生殖和神经系统的影响
Q4 Medicine Pub Date : 2023-08-30 DOI: 10.47470/0016-9900-2023-102-7-648-652
V. Vokina, L. Sosedova, M. A. Novikov, E. Andreeva, E. Titov, V. Rukavishnikov, M. Savchenkov
Introduction. The regular occurrence of large-scale peat fires is an urgent medical and environmental problem of the modern scientific community. The peat smoke is a complex mixture of pollutants that have a serious impact on air quality and public health. Materials and methods. Adult male rats were exposed to peat smoke for 24 hours, the concentrations of PM2.5 and CO were 0.92±0.08 mg/m3 and 40.8±1.9 mg/m3, respectively. After exposure an open field examination, EEG examination and histological analysis of the testes and brain were performed, after which they were mated with intact females. Postnatal death of offspring in the first week of life, body weight, behaviour and electroencephalography indicators during puberty were evaluated. Results. There was showed an increase in motor activity, anxiety, and violation of EEG indicators in rat males exposed to peat smoke. Both an increase in shadow cells and degeneratively altered neurons in the nervous tissue were found. No changes were detected in the testes. Exposure to peat smoke during conception increased stillbirth and mortality of offspring during the first week of life, but did not affect weight gain. Violation of behaviour structure was recorded only in females offspring. An increase in slow-wave activity of the brain in males offspring was revealed. Limitations. The study was limited to studying the effects of a single 24-hour exposure to peat smoke on outbred white rats. Conclusion. A single peat smoke exposure for 24 hours at a concentration of PM2.5=0.92±0.08 mg/m3 and CO=40.8±1.9 mg/m3 leads to significant morphological and functional changes in the CNS. In the absence of a gonadotoxic effect, peat smoke exposure on males before mating causes specific changes in the behavior and bioelectrical activity of the brain of their offspring.
介绍。大规模泥炭火灾的经常性发生是现代科学界亟待解决的医学和环境问题。泥炭烟是一种复杂的污染物混合物,对空气质量和公众健康产生严重影响。材料和方法。成年雄性大鼠暴露于泥炭烟24小时,PM2.5和CO浓度分别为0.92±0.08 mg/m3和40.8±1.9 mg/m3。暴露后进行野外检查、脑电图检查和睾丸和大脑的组织学分析,然后与完整的雌性交配。评估了出生后第一周的后代出生后死亡、体重、青春期的行为和脑电图指标。结果。暴露于泥炭烟雾的雄性大鼠的运动活动、焦虑和脑电图指标增高。在神经组织中发现了影子细胞的增加和退化性改变的神经元。睾丸未发现任何变化。在怀孕期间接触泥炭烟雾会增加死产和出生后第一周的后代死亡率,但不会影响体重增加。违规行为结构仅在雌性后代中有记录。研究发现,雄性后代的大脑慢波活动有所增加。的局限性。这项研究仅限于研究24小时暴露在泥炭烟雾中对近亲繁殖的小白鼠的影响。结论。在PM2.5=0.92±0.08 mg/m3和CO=40.8±1.9 mg/m3的浓度下,连续24小时暴露于单一泥炭烟会导致中枢神经系统的形态和功能发生显著变化。在没有性腺毒性作用的情况下,雄性在交配前接触泥炭烟雾会导致后代的行为和大脑的生物电活动发生特定的变化。
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引用次数: 0
Distribution of polymorphic variants of the TNF-α and TNFRSF1α genes in miners with dust lung pathology 尘肺组织中TNF-α和TNFRSF1α基因多态性变异的分布
Q4 Medicine Pub Date : 2023-08-30 DOI: 10.47470/0016-9900-2023-102-7-670-674
A. G. Zhukova, A. S. Kazitskaya, T. K. Yadykina, T. D. Logunova
Introduction. Long-term exposure to coal-rock dust on the body causes the development of systemic inflammation, one of the development mechanisms of which is an increase in the production of pro-inflammatory cytokines as follows: TNF-α, IL-1β, IL-2, IL-6. The development and course of occupational pathology were shown to depend on the individual characteristics of the body of those who works in hazardous conditions. In this regard, it is necessary to establish the significance of the polymorphism of the TNF-α and TNFRSF1α genes in the development of dust lung pathology in workers of the main occupations of the mines in the South of Kuzbass. The aim of the study was to investigate the prevalence of polymorphic variants of the TNF-α (rs1800629) and TNFRSF1α (rs4149584) genes in miners with dust lung pathology. Materials and methods. A survey of one hundred twenty seven miners working for a long time in the mines of the south of Kuzbass was carried out. Of these: 69 miners with prolonged exposure to high concentrations of coal-rock dust with a previously proven diagnosis of dust lung pathology, 58 miners without a proven diagnosis of dust lung pathology working in the same sanitary and hygienic conditions (control). The typing of the TNF-α (rs1800629) and TNFRSF1α (rs4149584) genes was carried out using the Real-Time method. Results. The GG rs1800629 TNF-α genotype were shown to be a potent molecular genetic marker of the risk of developing dust lung pathology, the chance of detecting it in the group of the miners with dust pathology is almost 4 times higher than in the control group. The carriage of the A allele and the heterozygous AG rs1800629 TNF-α genotype reduce the probability of developing dust lung pathology. It has been established that the GG rs4149584 TNFRSF1α genotype can be a molecular and genetic marker of the risk of developing dust lung pathology, the chance of detecting it in the group of the miners with dust pathology is 2.6 times higher than in the control group. Limitations. The study was limited by relatively small size of the groups under study. Conclusion. The carriage of homozygous GG genotypes of the rs1800629 and rs4149584 polymorphic loci of the TNF-α and TNFRSF1α genes, respectively, is associated with the risk of developing dust lung pathology in miners in the South of Kuzbass. The carriage of the A allele and the heterozygous AG rs1800629 TNF-α genotype reduce the probability of developing dust lung pathology.
介绍长期暴露在身上的煤尘会导致全身炎症的发展,其发展机制之一是促炎细胞因子的产生增加,如下:TNF-α、IL-1β、IL-2、IL-6。职业病理学的发展和过程取决于那些在危险条件下工作的人的身体特征。在这方面,有必要确定TNF-α和TNFRSF1α基因多态性在库兹巴斯南部矿山主要职业工人尘肺病理发展中的意义。本研究的目的是调查尘肺病理矿工中TNF-α(rs1800629)和TNFRSF1α(rs4149584)基因多态性变体的患病率。材料和方法。对在库兹巴斯南部矿山长期工作的127名矿工进行了调查。其中:69名长期暴露于高浓度煤岩粉尘的矿工先前被证实患有尘肺病理,58名未被证实患有粉尘肺病理的矿工在相同的卫生条件下工作(对照)。α(rs1800629)和TNFRSF1α(rs4149584)基因的实时分型。后果GG rs1800629 TNF-α基因型被证明是发展为尘肺病理风险的有效分子遗传标记,在有尘肺病理的矿工组中检测到它的几率几乎是对照组的4倍。携带A等位基因和杂合子AG rs1800629 TNF-α基因型可降低患尘肺疾病的概率。GG rs4149584 TNFRSF1α基因型可作为发生尘肺病理风险的分子和遗传标记,在有尘肺病理的矿工组中检测到它的几率是对照组的2.6倍。局限性这项研究受到研究小组规模相对较小的限制。结论分别携带TNF-α和TNFRSF1α基因的rs1800629和rs4149584多态位点的纯合GG基因型与库兹巴斯南部矿工患尘肺病理的风险有关。携带A等位基因和杂合子AG rs1800629 TNF-α基因型可降低患尘肺疾病的概率。
{"title":"Distribution of polymorphic variants of the TNF-α and TNFRSF1α genes in miners with dust lung pathology","authors":"A. G. Zhukova, A. S. Kazitskaya, T. K. Yadykina, T. D. Logunova","doi":"10.47470/0016-9900-2023-102-7-670-674","DOIUrl":"https://doi.org/10.47470/0016-9900-2023-102-7-670-674","url":null,"abstract":"Introduction. Long-term exposure to coal-rock dust on the body causes the development of systemic inflammation, one of the development mechanisms of which is an increase in the production of pro-inflammatory cytokines as follows: TNF-α, IL-1β, IL-2, IL-6. The development and course of occupational pathology were shown to depend on the individual characteristics of the body of those who works in hazardous conditions. In this regard, it is necessary to establish the significance of the polymorphism of the TNF-α and TNFRSF1α genes in the development of dust lung pathology in workers of the main occupations of the mines in the South of Kuzbass. \u0000The aim of the study was to investigate the prevalence of polymorphic variants of the TNF-α (rs1800629) and TNFRSF1α (rs4149584) genes in miners with dust lung pathology. \u0000Materials and methods. A survey of one hundred twenty seven miners working for a long time in the mines of the south of Kuzbass was carried out. Of these: \u000069 miners with prolonged exposure to high concentrations of coal-rock dust with a previously proven diagnosis of dust lung pathology, 58 miners without a proven diagnosis of dust lung pathology working in the same sanitary and hygienic conditions (control). The typing of the TNF-α (rs1800629) and TNFRSF1α (rs4149584) genes was carried out using the Real-Time method. \u0000Results. The GG rs1800629 TNF-α genotype were shown to be a potent molecular genetic marker of the risk of developing dust lung pathology, the chance of detecting it in the group of the miners with dust pathology is almost 4 times higher than in the control group. The carriage of the A allele and the heterozygous AG rs1800629 TNF-α genotype reduce the probability of developing dust lung pathology. It has been established that the GG rs4149584 TNFRSF1α genotype can be a molecular and genetic marker of the risk of developing dust lung pathology, the chance of detecting it in the group of the miners with dust pathology is 2.6 times higher than in the control group. \u0000Limitations. The study was limited by relatively small size of the groups under study. \u0000Conclusion. The carriage of homozygous GG genotypes of the rs1800629 and rs4149584 polymorphic loci of the TNF-α and TNFRSF1α genes, respectively, is associated with the risk of developing dust lung pathology in miners in the South of Kuzbass. The carriage of the A allele and the heterozygous AG rs1800629 TNF-α genotype reduce the probability of developing dust lung pathology.","PeriodicalId":12550,"journal":{"name":"Gigiena i sanitariia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46521688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Features of the use of ischemic preconditioning for the correction of vibration polyneuropathy of the upper extremities 缺血预处理治疗上肢振动性多发性神经病的特点
Q4 Medicine Pub Date : 2023-08-30 DOI: 10.47470/0016-9900-2023-102-7-695-699
A. V. Yamshchikova, A. Fleishman, I. Martynov, Tatiana A. Bychkovskaya, A. A. Kungurova
Introduction. The study of the methods for non-pharmacological correction of vibration polyneuropathy is relevant in view of the high prevalence of vibration disease in various industries, and high occurrence of disability of workers. Ischemic preconditioning has shown its effectiveness in correcting the manifestations of vibration polyneuropathies. The high frequency of nerve compression in tunnels against the background of polyneuropathic lesions in vibration disease dictates the need to study the effectiveness of the method in various forms of vibration polyneuropathy. The aim of the study was to investigate the effectiveness of ischemic preconditioning in various forms of vibration polyneuropathy. Materials and methods. Twenty nine patients with a proven diagnosis of vibration disease were examined and treated uisng the ischemic preconditioning. They were divided into 2 groups: group 1 – 18 individuals with upper extremities polyneuropathy without nerve compression, group 2 – 11 subjects with compression complications of polyneuropathy. The examinations included neurological survey, electroneuromyographic investigation before and after the course of ischemic preconditioning conducted according to a patented technique. Results. A decrease in sensory disturbances and improvement in the impulse conduction along the sensory fibers of the nerves of the upper extremities after a course of ischemic preconditioning in the group of the patients without compression neuropathies were noted. In the group of the patients with polyneuropathies complicated by the nerve compression in tunnels, the positive effect was less significant. Limitations. The study was limited by the assessment of clinical and electroneuromyographic parameters in 29 patients with vibration disease due to the exposure to local vibration before and after the course of ischemic preconditioning. Conclusion. The method of ischemic preconditioning is effective as a method for non-pharmacological correction of vibration polyneuropathy. Compression neuropathies are predictors of lower efficiency of the method, and therefore it is important to select patients for the treatment by this method with the preferred inclusion of the patients with uncomplicated vibration polyneuropathies.
介绍鉴于振动性多发性神经病在各个行业的高发病率和工人残疾的高发生率,对振动性多发性神经病的非药物治疗方法的研究是相关的。缺血预处理已显示其在纠正振动性多发性神经病表现方面的有效性。在振动性疾病中多发性神经病病变的背景下,隧道中神经压迫的频率很高,因此需要研究该方法在各种形式的振动性多发性病变中的有效性。本研究的目的是研究缺血预处理在各种形式的振动性多发性神经病中的有效性。材料和方法。对29例确诊为振动性疾病的患者进行了检查,并用缺血预处理进行了治疗。他们被分为2组:第1-18组为无神经压迫的上肢多发性神经病患者,第2-11组为有多发性病变压迫并发症的受试者。检查包括神经系统调查、根据专利技术进行的缺血预处理前后的神经肌电图检查。后果在没有压迫性神经病的患者组中,缺血预处理一个疗程后,感觉障碍减少,沿上肢神经感觉纤维的脉冲传导改善。在多发性神经病合并隧道内神经压迫的患者组中,积极作用不太显著。局限性该研究受到29名振动性疾病患者的临床和神经肌电图参数评估的限制,这些患者在缺血预处理前后暴露于局部振动。结论缺血预处理方法是一种有效的非药物治疗振动性多发性神经病的方法。压迫性神经病是该方法效率较低的预测因素,因此,选择通过该方法进行治疗的患者是重要的,优选包括无并发症的振动性多发性神经病患者。
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引用次数: 0
Hygienic assessment of mycological contamination of the internal environment of residential and public buildings 住宅和公共建筑内部环境真菌污染的卫生评价
Q4 Medicine Pub Date : 2023-08-30 DOI: 10.47470/0016-9900-2023-102-7-632-638
N. Kalinina, A. V. Zagainova, S. Yudin, E. B. Gaponova, I. M. Banin
The paper presents the results of a comprehensive hygienic assessment of the fungal contamination of the internal environment of the premises of residential and public buildings and developed proposals for the methodology for conducting mycological examination of indoor air and building structures. Materials and methods. The objects of research were urban atmospheric air, residential apartments, and office premises with and without fungal infection of wall structures, medical premises for various purposes. The studies included determination of the number of microscopic fungi in 1 m3 of air (CFU/m3); determination of the content of viable fungal flora in scrapings and in swabs from places suspected of having a fungal infection; measurements of humidity of enclosing structures and microclimatic parameters of the indoor air environment; evaluation of the ventilation system. Results. The concentration of microscopic fungi in indoor air without fungal infection of wall structures was shown to depend on fungal pollution of atmospheric air. The peak of fungal air pollution in unaffected rooms and the ambient air, was detected over the spring-summer season. In residential apartments and office premises of public buildings with fungal infection of wall structures, the content of microscopic fungi in the air, regardless of the season, was tens and hundreds of times higher than the content of fungal flora in the air of “healthy” premises and in the atmospheric air. Fungal air pollution in residential and office premises is mainly associated with fungi of the Penicillium, Aspergillus, Cladosporium and Rizopus genera in wards, treatment and diagnostic rooms – yeast-like fungi of the genus Candida. Limitations. The results of the study do not apply to the production facilities of biological industry enterprises, livestock and poultry complexes. Conclusion. The necessity of conducting a mycological examination of premises has been substantiated, and a draft of methodological recommendations for conducting mycological examinations of indoor air and building structures has been developed.
本文介绍了对住宅和公共建筑内部环境真菌污染的综合卫生评估结果,并提出了对室内空气和建筑结构进行真菌检查的方法建议。材料和方法。研究对象是城市大气、住宅、办公场所的墙壁结构是否有真菌感染,以及用于各种目的的医疗场所。这些研究包括测定1立方米空气中微观真菌的数量(CFU/m3);确定疑似有真菌感染的地方的刮片和拭子中活真菌菌群的含量;封闭结构的湿度和室内空气环境的小气候参数的测量;通风系统的评估。后果室内空气中微观真菌的浓度与大气中真菌的污染有关。未受影响的房间和环境空气中的真菌空气污染高峰出现在春夏季节。在墙壁结构受到真菌感染的住宅公寓和公共建筑的办公场所,无论季节如何,空气中微观真菌的含量都比“健康”场所空气和大气中真菌菌群的含量高出数十倍和数百倍。住宅和办公场所的真菌空气污染主要与病房、治疗和诊断室中的青霉属、曲霉菌属、枝孢霉属和Rizopus属真菌有关——念珠菌属的酵母样真菌。局限性研究结果不适用于生物工业企业、畜禽综合体的生产设施。结论对房屋进行真菌学检查的必要性已经得到证实,并制定了对室内空气和建筑结构进行真菌病检查的方法建议草案。
{"title":"Hygienic assessment of mycological contamination of the internal environment of residential and public buildings","authors":"N. Kalinina, A. V. Zagainova, S. Yudin, E. B. Gaponova, I. M. Banin","doi":"10.47470/0016-9900-2023-102-7-632-638","DOIUrl":"https://doi.org/10.47470/0016-9900-2023-102-7-632-638","url":null,"abstract":"The paper presents the results of a comprehensive hygienic assessment of the fungal contamination of the internal environment of the premises of residential and public buildings and developed proposals for the methodology for conducting mycological examination of indoor air and building structures. \u0000Materials and methods. The objects of research were urban atmospheric air, residential apartments, and office premises with and without fungal infection of wall structures, medical premises for various purposes. The studies included determination of the number of microscopic fungi in 1 m3 of air (CFU/m3); determination of the content of viable fungal flora in scrapings and in swabs from places suspected of having a fungal infection; measurements of humidity of enclosing structures and microclimatic parameters of the indoor air environment; evaluation of the ventilation system. \u0000Results. The concentration of microscopic fungi in indoor air without fungal infection of wall structures was shown to depend on fungal pollution of atmospheric air. The peak of fungal air pollution in unaffected rooms and the ambient air, was detected over the spring-summer season. In residential apartments and office premises of public buildings with fungal infection of wall structures, the content of microscopic fungi in the air, regardless of the season, was tens and hundreds of times higher than the content of fungal flora in the air of “healthy” premises and in the atmospheric air. Fungal air pollution in residential and office premises is mainly associated with fungi of the Penicillium, Aspergillus, Cladosporium and Rizopus genera in wards, treatment and diagnostic rooms – yeast-like fungi of the genus Candida. \u0000Limitations. The results of the study do not apply to the production facilities of biological industry enterprises, livestock and poultry complexes. \u0000Conclusion. The necessity of conducting a mycological examination of premises has been substantiated, and a draft of methodological recommendations for conducting mycological examinations of indoor air and building structures has been developed.","PeriodicalId":12550,"journal":{"name":"Gigiena i sanitariia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49583778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of the neurotoxic effect of iron (III) oxide nanoparticles at the subcellular level 在亚细胞水平上评估氧化铁纳米颗粒的神经毒性作用
Q4 Medicine Pub Date : 2023-08-30 DOI: 10.47470/0016-9900-2023-102-7-720-725
I. G. Shelomentsev, Lev A. Amromin, D. Shaikhova, M. Sutunkova, I. Minigalieva
Introduction. Both naturally occurring and artificially produced nanoparticles are ubiquitous; their high concentrations can be detected in the environment, thus posing risks of toxic effects in humans. Penetrating the blood-brain barrier by metal nanoparticles has been already proven and is currently of interest from the point of view of toxicology and hygiene. Materials and methods. Female rats were exposed to ferric oxide nanoparticles administered intranasally with a 25 mg/ml suspension at a dose of 50 µl three times a week during six weeks. The experimental and control groups contained seven animals each. Tissue samples for testing were taken from the olfactory bulbs of the rat’s brain. Iron (III) oxide nanoparticles were identified by electron microscopy and energy-dispersive X-ray spectroscopy. The cytotoxic effect of ferric oxide nanoparticles was assessed by ranking mitochondria by mitochondrial membrane morphotypes and comparing their distribution in the experimental and control groups. Results. We confirmed the presence of nanoparticles in tissues of the olfactory bulbs of the exposed rodents. The morphotype pattern of mitochondria showed significant changes following the exposure to ferric oxide nanoparticles: the proportion of mitochondria with normal and vesicular swollen morphotypes decreased by 36.4 and 4.9%, respectively, compared with the control group of animals, the proportion of mitochondria with normal vesicular and vesicular morphotypes increased by 19.8 and 21.8%, while the proportion of vesicular swollen mitochondria decreased from 9.5% to 4.6%. Limitations. The study was limited to examining ultrastructural changes in mitochondria and identifying ferric oxide nanoparticles in tissues. Conclusions. Further studies of the impact of iron-containing nanoparticles on the structure and functions of the mitochondrial apparatus can help to identify their potential harm at the subcellular level and provide information for the development of appropriate health protective measures and new strategies for prevention and treatment of metal toxicity-induced diseases in humans.
介绍。自然产生的和人工制造的纳米颗粒无处不在;在环境中可以检测到它们的高浓度,从而对人类造成毒性影响的风险。金属纳米颗粒穿透血脑屏障已经得到证实,目前从毒理学和卫生学的角度来看,这是一个有趣的研究方向。材料和方法。雌性大鼠暴露于含25 mg/ml悬浮液的氧化铁纳米颗粒鼻内给药,剂量为50 μ l,每周三次,持续六周。实验组和对照组各7只。用于测试的组织样本取自大鼠大脑的嗅球。利用电子显微镜和能量色散x射线光谱对氧化铁纳米颗粒进行了鉴定。通过对线粒体膜形态进行排序,并比较实验组和对照组线粒体膜的分布情况,评估氧化铁纳米颗粒的细胞毒性作用。结果。我们证实在暴露的啮齿动物的嗅球组织中存在纳米颗粒。氧化铁纳米颗粒暴露后,线粒体形态模式发生了显著变化:正常和水疱肿胀型线粒体比例分别下降了36.4%和4.9%,与对照组相比,正常水疱和水疱型线粒体比例分别上升了19.8%和21.8%,水疱肿胀型线粒体比例从9.5%下降到4.6%。的局限性。该研究仅限于检查线粒体的超微结构变化和确定组织中的氧化铁纳米颗粒。结论。进一步研究含铁纳米颗粒对线粒体结构和功能的影响,有助于确定其在亚细胞水平上的潜在危害,并为制定适当的健康保护措施和预防和治疗人类金属中毒引起的疾病的新策略提供信息。
{"title":"Assessment of the neurotoxic effect of iron (III) oxide nanoparticles at the subcellular level","authors":"I. G. Shelomentsev, Lev A. Amromin, D. Shaikhova, M. Sutunkova, I. Minigalieva","doi":"10.47470/0016-9900-2023-102-7-720-725","DOIUrl":"https://doi.org/10.47470/0016-9900-2023-102-7-720-725","url":null,"abstract":"Introduction. Both naturally occurring and artificially produced nanoparticles are ubiquitous; their high concentrations can be detected in the environment, thus posing risks of toxic effects in humans. Penetrating the blood-brain barrier by metal nanoparticles has been already proven and is currently of interest from the point of view of toxicology and hygiene. \u0000Materials and methods. Female rats were exposed to ferric oxide nanoparticles administered intranasally with a 25 mg/ml suspension at a dose of 50 µl three times a week during six weeks. The experimental and control groups contained seven animals each. Tissue samples for testing were taken from the olfactory bulbs of the rat’s brain. Iron (III) oxide nanoparticles were identified by electron microscopy and energy-dispersive X-ray spectroscopy. The cytotoxic effect of ferric oxide nanoparticles was assessed by ranking mitochondria by mitochondrial membrane morphotypes and comparing their distribution in the experimental and control groups. \u0000Results. We confirmed the presence of nanoparticles in tissues of the olfactory bulbs of the exposed rodents. The morphotype pattern of mitochondria showed significant changes following the exposure to ferric oxide nanoparticles: the proportion of mitochondria with normal and vesicular swollen morphotypes decreased by 36.4 and 4.9%, respectively, compared with the control group of animals, the proportion of mitochondria with normal vesicular and vesicular morphotypes increased by 19.8 and 21.8%, while the proportion of vesicular swollen mitochondria decreased from 9.5% to 4.6%. \u0000Limitations. The study was limited to examining ultrastructural changes in mitochondria and identifying ferric oxide nanoparticles in tissues. \u0000Conclusions. Further studies of the impact of iron-containing nanoparticles on the structure and functions of the mitochondrial apparatus can help to identify their potential harm at the subcellular level and provide information for the development of appropriate health protective measures and new strategies for prevention and treatment of metal toxicity-induced diseases in humans.","PeriodicalId":12550,"journal":{"name":"Gigiena i sanitariia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45543696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Scientific substantiation to updating drinking water quality monitoring programs 更新饮用水质量监测项目的科学依据
Q4 Medicine Pub Date : 2023-07-30 DOI: 10.47470/0016-9900-2023-102-6-544-548
Y. Novikova, N. Tikhonova, V. Fedorov, O. Kopytenkova, I. O. Myasnikov, Olga S. Alenteva, K. B. Friedman
Introduction. The results of systematic drinking water quality laboratory tests are the most informative sources. Recommendations on the managing of drinking water quality monitoring were developed in 2020. The purpose of the study was to substantiate methodological approaches to updating drinking water quality monitoring programs. Materials and methods. The results of drinking water quality laboratory tests in the Murmansk region, conducted in 2021 in accordance with the monitoring program, were used. The results were analyzed for compliance with the recommendations of the drinking water quality monitoring program. An assessment of the compliance of the results of laboratory studies with hygienic standards and public health risk. Results. In Monchegorsk, the drinking water quality was studied at nine control points according to sanitary-chemical, microbiological, and parasitological indicators. The multiplicity of studies per year ranged from 1 to 12 studies. The quality of drinking water in Monchegorsk corresponded to hygienic standards, with the exception of single excesses in the content of chlorine residual free and colour. Limitations. When conducting the study, the results of the monitoring were used only for 2021, the results of drinking water quality production control were not taken into account. Conclusion. To carry out an objective drinking water quality assessment, it is necessary to update the existing programs of monitoring its quality in the settlements of the Murmansk region carried out within the framework of the social hygienic monitoring (SHM). The authors propose an algorithm for updating monitoring programs
介绍。系统的饮用水质量实验室检测的结果是最有信息的来源。2020年制定了关于管理饮用水质量监测的建议。该研究的目的是证实更新饮用水质量监测项目的方法学方法。材料和方法。使用了2021年根据监测方案在摩尔曼斯克地区进行的饮用水质量实验室测试的结果。分析结果是否符合饮用水质量监测方案的建议。对实验室研究结果是否符合卫生标准和公共健康风险的评估。结果。在蒙切戈尔斯克,根据卫生化学、微生物学和寄生虫学指标,在9个控制点对饮用水质量进行了研究。每年的研究数量从1到12个不等。Monchegorsk的饮用水质量符合卫生标准,但氯残留量和颜色超标。的局限性。在进行研究时,监测结果仅用于2021年,未考虑饮用水质量生产控制的结果。结论。为了进行客观的饮用水质量评估,有必要在社会卫生监测(SHM)框架内更新摩尔曼斯克地区住区现有的水质监测方案。作者提出了一种更新监测程序的算法
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引用次数: 0
Features of emotional burnout of employees of preschool educational institutions 学前教育机构员工情绪倦怠的特征
Q4 Medicine Pub Date : 2023-07-30 DOI: 10.47470/0016-9900-2023-102-6-573-579
T. V. Temaev, Irina V. Temaeva, A. D. Trubetskov, Nataliia E. Komleva, Darya A. Chuslyaeva
Introduction. In this article, on the basis of quantitative and qualitative research methods, the emotional burnout of day-care assistants and speech therapists of municipal preschool educational institutions was studied. In recent years, there has been an increase in the importance of the psycho-emotional component of the professional portrait of an employee of preschool educational institutions. The content of pedagogical professional activity includes many factors that provoke the emergence of professional deformation of a specialist. Materials and methods. Emotional burnout (according to the method of K. Maslach and S. Jackson in the adaptation of N. Vodopyanova) and the working capacity index (WAI) were studied in day-care assistants and speech therapists working in preschool educational institutions, as part of a cross-sectional study, in-depth interviewing was conducted. The SPSS 23 software package was used for statistical analysis. Results. Day-care assistants and speech therapists of preschool educational institutions, who have a long work experience, have fully realized and adapted to their profession and do not note emotional and psychological overloads. The results of a qualitative study indicate the dominance of subject-object (educator – child) relationships within the framework of preschool education, the presence of a complex psychological space (preschool is visited by children with various psychophysiological, emotional, behavioral characteristics), the settling of negative emotions inside the personal space of the educator and the speech therapist teacher and, as a consequence, the inability to dispose of them (personal psychotherapy, supervision, professional intervention) without negative consequences. Limitations. The study has professional (day-care assistants, speech therapists) limitations. Conclusion. These results suggest the problem of emotional burnout to be quite deep and not realized by teachers and day-care assitants. The resources of quantitative research methods for educators and speech therapists of preschool education on the topic of professional burnout and deformation were shown to be significantly limited and fail to allow achieving objective results, which requires the development of new approaches to assessing teachers’ emotional burnout.
介绍。本文采用定量与定性相结合的研究方法,对市级幼教机构日托助理和语言治疗师的情绪倦怠进行了研究。近年来,学前教育机构员工职业形象的心理情感成分的重要性有所增加。教学专业活动的内容包含了引发专家专业变形的诸多因素。材料和方法。本文采用横断面研究方法,采用深度访谈法,研究了学前教育机构日托助理和言语治疗师的情绪倦怠(参照K. Maslach和S. Jackson在《N. Vodopyanova》中的适应方法)和工作能力指数(WAI)。采用SPSS 23软件包进行统计分析。结果。幼教机构的日托员和言语治疗师工作经验较长,对自己的职业有充分的认识和适应,没有出现情绪和心理上的超负荷。一项定性研究的结果表明,在学前教育的框架内,主体-客体(教育者-儿童)关系占主导地位,存在复杂的心理空间(学龄前是由具有各种心理生理、情感和行为特征的儿童访问的),负面情绪在教育者和语言治疗师教师的个人空间内沉淀,因此,无法处理它们(个人心理治疗,监督,专业干预)没有负面后果。的局限性。这项研究有专业人员(日托助理、语言治疗师)的限制。结论。这些结果表明,情绪倦怠的问题是相当深刻的,没有意识到教师和日托助理。学前教育教育工作者和语言治疗师在职业倦怠和变形方面的定量研究方法资源明显有限,无法取得客观结果,这就需要开发新的方法来评估教师的情绪倦怠。
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引用次数: 0
On the need to increase the age categories of male military personnel for rationing of the nutritional status 关于需要增加男性军事人员年龄类别以进行营养状况配给的问题
Q4 Medicine Pub Date : 2023-07-30 DOI: 10.47470/0016-9900-2023-102-6-584-590
G. Smirnova, E. V. Kravchenko
Introduction. The purpose of the study is scientific and methodological justification of the need to increase the number of age groups to clarify the standards for assessing the nutritional status of military personnel. Materials and methods. The study involved one thousand three hundred seventy six 18 to 53 years male servicemen under medical examination. The following methods were used: anthropometry, assessing body composition (bioimpedance, caliperometry), the functional state and adaptive reserves. Statistical data analysis was carried out using Microsoft Excel and Statistica 10.0 software products. Results. Based on the results of the analysis of literary sources and own research, for assessing and rationing of the nutritional status there were identified 4 age groups of military personnel, differed from each other at a highly reliable level (p>0.001) for all the studied indicators. It was found that representatives of the 1st age group (18–20 years) were characterized by the best values of adaptation of the cardiovascular system and the body’s resistance to physical exertion, as well as high reserve functionality and recovery rate after physical exertion. Representatives of the 2nd age group (21–27 years old) are objectively the best in most of the indicators studied by us compared to other age groups. In the military personnel of the 3rd age group (28–35 years), in general, with satisfactory results for most of the parameters studied, there was a significant decrease in the indicators of the functional state, compared with the first two groups. Representatives of the 4th age group (36 years and over) had the highest percentage of values of the studied indicators below the norm. Limitations. The medical examination, which included measurements of body size, composition, and assessment of functional status, involved 1,376 18 to 53 years male servicemen, which is a sufficient reference sample. Conclusion. The necessity of increasing the number of age groups to assess the nutritional status in male servicemen is substantiated. Based on the conducted research, 4 age groups of military personnel were identified to assess the nutritional status, which differ from each other at a highly reliable level.
介绍。这项研究的目的是从科学和方法上证明有必要增加年龄组的数目,以澄清评估军事人员营养状况的标准。材料和方法。这项研究对1376名18至53岁的男性军人进行了体检。采用以下方法:人体测量,评估身体成分(生物阻抗,体尺测量),功能状态和适应性储备。统计数据分析采用Microsoft Excel和Statistica 10.0软件产品。结果。根据文献资料分析和自己的研究结果,在营养状况评估和配给方面,确定了4个军人年龄组,所有研究指标在高可靠水平上存在差异(p < 0.001)。研究发现,第1年龄组(18-20岁)具有心血管系统适应性和机体抵抗体力消耗的最佳值,体力消耗后的储备功能和恢复率较高。与其他年龄组相比,第二年龄组的代表(21-27岁)在我们研究的大多数指标中客观上都是最好的。在第三年龄组(28-35岁)的军人中,总的来说,大多数研究参数的结果令人满意,但与前两组相比,功能状态指标明显下降。第4年龄组(36岁及以上)的代表所研究的指标值低于标准的百分比最高。的局限性。体格检查包括测量体型、身体成分和评估功能状况,涉及1 376名18至53岁的男性军人,这是一个足够的参考样本。结论。增加男性军人营养状况评估年龄组的必要性得到了证实。根据所进行的研究,确定了4个年龄组的军人营养状况评估,这些年龄组在高可靠水平上存在差异。
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引用次数: 0
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Gigiena i sanitariia
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