Pub Date : 2022-11-08DOI: 10.14710/baf.7.2.2022.120-125
E. Ernawiati, Lili Chrisnawati
Pisang Kepok memiliki keragaman aksesi/kultivar yang tinggi, namun pengembangan kultivar belum banyak dikaji. Penelitian ini bertujuan mengidentifikasikan struktur anatomi daun sebagai karakter penanda pisang Kepok poliploid dengan pemberian ekstrak umbi kembang sungsang sebagai mutagen alami untuk pengembangan kultivar unggul melalui perakitan secara in vitro. Penelitian disusun dalam Rancangan Acak Lengkap dengan dua faktor. Faktor 1 terdiri dari ekstrak air umbi kembang sungsang (10%), larutan kolkisin murni (0,1%) sebagai kontrol positif dan tanpa diberi penambahan (0%) sebagai kontrol negatif. Faktor 2 terdiri dari Kepok Abu, Kepok Batu dan Kepok Kuning. Semua kombinasi perlakuan diulang 5 kali. Data dianalisis ANAVA dan jika ada perbedaan dilanjutkan dengan uji DMRT pada taraf 5%. Hasil pengamatan menunjukkan pemberian kolkisin pada media kultur dapat menginduksi munculnya planlet pisang poliploid jika dilihat dari penambahan ukuran sel epidermis dan stomata serta menurunkan indeks stomata. Namun, ekstrak umbi kembang sungsang 10% belum mampu menginduksi munculnya planlet poliploid. Pisang Kepok kuning memiliki respon lebih baik terhadap pemberian mutagen dibandingkan Kepok abu dan batu. Dari hasil tersebut dapat disimpulkan bahwa kolkisin 0,1% mampu menginduksi planlet pisang Kepok poliploid. Pisang Kepok has a high diversity of accessions/cultivars, but cultivar development has not been widely studied. This study aims to identify the anatomical structure of the leaves as markers of polyploid Kepok bananas with the addition of flame lily tuber extract as a natural mutagen for the development of superior cultivars through in vitro assembly. The study was arranged in a completely randomized design with two factors. Factor 1 consisted of water extract of flame lily bulbs (10%), pure colchicine solution (0.1%) as a positive control and no addition (0%) as a negative control. Factor 2 consists of Kepok Abu, Kepok Batu and Kepok Kuning. All treatment combinations were repeated 5 times. The data were analyzed by ANAVA and if there was a difference, it was continued with the DMRT test at the 5% level. The results showed that the addition of colchicine to the culture media could induce the emergence of polyploid banana plantlets when observed from the increase in the size of the epidermal and stomata cells and decrease the stomatal index. However, 10% flame lily tuber extract was not able to induce the emergence of polyploid plantlets. Kepok Kuning bananas had a better response to mutagens than Kepok Abu and Kepok Batu bananas. From these results it can be concluded that 0.1% colchicine was able to induce polyploid Kepok banana plantlets.
{"title":"Profil Anatomi Daun Planlet Pisang Kepok Hasil Induksi Mutagen Alami Secara In Vitro","authors":"E. Ernawiati, Lili Chrisnawati","doi":"10.14710/baf.7.2.2022.120-125","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.120-125","url":null,"abstract":"Pisang Kepok memiliki keragaman aksesi/kultivar yang tinggi, namun pengembangan kultivar belum banyak dikaji. Penelitian ini bertujuan mengidentifikasikan struktur anatomi daun sebagai karakter penanda pisang Kepok poliploid dengan pemberian ekstrak umbi kembang sungsang sebagai mutagen alami untuk pengembangan kultivar unggul melalui perakitan secara in vitro. Penelitian disusun dalam Rancangan Acak Lengkap dengan dua faktor. Faktor 1 terdiri dari ekstrak air umbi kembang sungsang (10%), larutan kolkisin murni (0,1%) sebagai kontrol positif dan tanpa diberi penambahan (0%) sebagai kontrol negatif. Faktor 2 terdiri dari Kepok Abu, Kepok Batu dan Kepok Kuning. Semua kombinasi perlakuan diulang 5 kali. Data dianalisis ANAVA dan jika ada perbedaan dilanjutkan dengan uji DMRT pada taraf 5%. Hasil pengamatan menunjukkan pemberian kolkisin pada media kultur dapat menginduksi munculnya planlet pisang poliploid jika dilihat dari penambahan ukuran sel epidermis dan stomata serta menurunkan indeks stomata. Namun, ekstrak umbi kembang sungsang 10% belum mampu menginduksi munculnya planlet poliploid. Pisang Kepok kuning memiliki respon lebih baik terhadap pemberian mutagen dibandingkan Kepok abu dan batu. Dari hasil tersebut dapat disimpulkan bahwa kolkisin 0,1% mampu menginduksi planlet pisang Kepok poliploid. Pisang Kepok has a high diversity of accessions/cultivars, but cultivar development has not been widely studied. This study aims to identify the anatomical structure of the leaves as markers of polyploid Kepok bananas with the addition of flame lily tuber extract as a natural mutagen for the development of superior cultivars through in vitro assembly. The study was arranged in a completely randomized design with two factors. Factor 1 consisted of water extract of flame lily bulbs (10%), pure colchicine solution (0.1%) as a positive control and no addition (0%) as a negative control. Factor 2 consists of Kepok Abu, Kepok Batu and Kepok Kuning. All treatment combinations were repeated 5 times. The data were analyzed by ANAVA and if there was a difference, it was continued with the DMRT test at the 5% level. The results showed that the addition of colchicine to the culture media could induce the emergence of polyploid banana plantlets when observed from the increase in the size of the epidermal and stomata cells and decrease the stomatal index. However, 10% flame lily tuber extract was not able to induce the emergence of polyploid plantlets. Kepok Kuning bananas had a better response to mutagens than Kepok Abu and Kepok Batu bananas. From these results it can be concluded that 0.1% colchicine was able to induce polyploid Kepok banana plantlets.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"79 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123140791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-30DOI: 10.14710/baf.7.2.2022.135-143
M. Djaelani, Kasiyati Kasiyati, S. Sunarno
Penelitian ini dirancang untuk melihat pengaruh aerasi dan kepadatan tempat hidup sebagai sumber stres terhadap pertumbuhan ikan nila merah (Oreochromis niloticus). Rancangan penelitian yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) faktorial dengan dua perlakuan, terdiri atas perlakuan dilengkapi dengan satu aerator dan perlakuan dolengkapi dengan dua aerator serta tiga tingkatan padat tebar 4,6,8 ekor ikan. Ikan nila merah dengan berat 4±0,5 g dipelihara dalam kontainer boks kapasitas 15 liter dengan volume air 8 liter. Ikan dipelihara selama 30 hari. Variabel yang diamati adalah bobot ikan, Panjang dan tinggi ikan, serta bobot karkas dan kandungan protein daging ikan. Faktor lingkungan yang diamati meliputi Oksigen terlarut (DO), pH, suhu, kandungan ammonia, nitrit dan nitrat. Analisis data dikerjakan menggunakan analisis sidik ragam (ANOVA) dua arah. Analisis data dikerjakan dengan program SPSS versi 23. Perbedaan yang nyata antar perlakuan diuji dengan uji Beda NyataTerkecil (BNT). Beda nyata dievaluasi pada taraf P < 0.05. Hasil penelitian menunjukkan peningkatan padat tebar sampai dengan dua kali lipat kapasitas normal dengan menggunakan satu aerator sebagai sumber oksigen terlarut menghambat pertumbuhan dan menurunkan bobot karkas dan kandungan protein daging ikan. Pada penelitian ini dapat disimpulkan bahwa padat tebar ikan masih bisa ditingkatkan sampai dengan dua kali lipat kapasitas normal dengan penambahan aerator sebagai peningkatan oksigen terlarut.This study was designed to examine the effect of aeration and density of living space as a source of stress on the growth of red tilapia (Oreochromis niloticus). The research design used in this study was a factorial Completely Randomized Design (CRD) with two treatments, consisting of treatment equipped with one aerator and treatment equipped with two aerator and three levels of stocking density of 4,6,8 fish. Red tilapia weighing 4±0.5 g was reared in a box container with a capacity of 15 liters with a volume of 8 liters of water. Fish were kept for 30 days. The variables observed were length and height of the fish,carcass weight and protein content of fish meat. Environmental factors observed included dissolved oxygen (DO), pH, temperature, ammonia, nitrite and nitrate content. Data analysis was carried out using two way analysis of variance (ANOVA). Data analysis was carried out using the SPSS version 23 program. Significant differences between treatments were tested using Least Significant Difference test (LSD). Significant differences were evaluated at the level of P < 0.05. The results showed an increase in stocking density up to twice the normal capacity by using an aerator as a source of dissolved oxygen inhibiting growth and reducing carcass weight and protein content of fish meat. In this study it can be concluded that the stocking density of fish can still be increased up to twice the normal capacity with the addition of an aerator as an increase in
这项研究的目的是观察红藻鱼(学名Oreochromis niloticus)生长所带来的压力。在本研究中使用的研究设计是一种完全随机的分层设计，由一种具有两种导体的治疗和一种具有两种导体和三层固体tebar 4.6.8条鱼组成。罗非鱼集装箱重量4±0.5 g中所孕育的红色盒子和水量15升容量8升。鱼可以养殖30天。观察到的变量包括鱼的体重、鱼的长度和高度、鱼肉的碳和蛋白质含量。观察到的环境因素包括溶解的氧气(DO)、pH、温度、氨、硝酸盐和硝酸盐。数据分析是通过双向指纹分析进行的。数据分析工作在SPSS版本23中。治疗方法的真正区别是用最小的蚊子测试(BNT)。P < 0.05级评价的真正差异。研究结果显示，单导管作为溶解氧气的来源，tebar的密度增加了一倍。在这项研究中，可以得出结论，河豚的密度仍然可以通过增加溶解的氧气来增加其正常容量的两倍。这项研究是为了检验活空间对大气和密度的影响，它是赤铁矿生长压力的源泉。这项研究中使用的研究发现是一种用两种方法进行的完整的转移设计，考虑到两种焊接和三层袜子的两种焊接和三层袜子的两种焊接。红罗非鱼weighing 4±0。5 g是reared in a box货柜with a capacity of 15升with a 8升的水的体积。鱼撑了30天。观察变化是鱼的长度和高度，carcass的重量和蛋白质组成的肉肉。环境因素观察包括分离氧、pH、温度、氨、硝酸盐和硝酸盐满足。数据分析采用两种变量分析方法进行分析。数据分析采用SPSS版本23个程序进行。treatments之间的重要区别使用了最重要的成分测试(LSD)测试。有争议的差异在P < 0.05的水平上被评估。替代品通过使用一种被稀释的氧气抑制剂和减少卡丝的分量和潜在的鱼肉蛋白质来显示正常电容器的增加。在这项研究中，它可以确定袜子的密度仍然可以增加到与越来越少的氢氧素的附加关系之间的可变电能。
{"title":"Pertumbuhan Ikan Nila Merah (Oreochromis niloticus) Pada Berbagai Padat Tebar Dan Dengan Penambahan Aerator","authors":"M. Djaelani, Kasiyati Kasiyati, S. Sunarno","doi":"10.14710/baf.7.2.2022.135-143","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.135-143","url":null,"abstract":"Penelitian ini dirancang untuk melihat pengaruh aerasi dan kepadatan tempat hidup sebagai sumber stres terhadap pertumbuhan ikan nila merah (Oreochromis niloticus). Rancangan penelitian yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) faktorial dengan dua perlakuan, terdiri atas perlakuan dilengkapi dengan satu aerator dan perlakuan dolengkapi dengan dua aerator serta tiga tingkatan padat tebar 4,6,8 ekor ikan. Ikan nila merah dengan berat 4±0,5 g dipelihara dalam kontainer boks kapasitas 15 liter dengan volume air 8 liter. Ikan dipelihara selama 30 hari. Variabel yang diamati adalah bobot ikan, Panjang dan tinggi ikan, serta bobot karkas dan kandungan protein daging ikan. Faktor lingkungan yang diamati meliputi Oksigen terlarut (DO), pH, suhu, kandungan ammonia, nitrit dan nitrat. Analisis data dikerjakan menggunakan analisis sidik ragam (ANOVA) dua arah. Analisis data dikerjakan dengan program SPSS versi 23. Perbedaan yang nyata antar perlakuan diuji dengan uji Beda NyataTerkecil (BNT). Beda nyata dievaluasi pada taraf P < 0.05. Hasil penelitian menunjukkan peningkatan padat tebar sampai dengan dua kali lipat kapasitas normal dengan menggunakan satu aerator sebagai sumber oksigen terlarut menghambat pertumbuhan dan menurunkan bobot karkas dan kandungan protein daging ikan. Pada penelitian ini dapat disimpulkan bahwa padat tebar ikan masih bisa ditingkatkan sampai dengan dua kali lipat kapasitas normal dengan penambahan aerator sebagai peningkatan oksigen terlarut.This study was designed to examine the effect of aeration and density of living space as a source of stress on the growth of red tilapia (Oreochromis niloticus). The research design used in this study was a factorial Completely Randomized Design (CRD) with two treatments, consisting of treatment equipped with one aerator and treatment equipped with two aerator and three levels of stocking density of 4,6,8 fish. Red tilapia weighing 4±0.5 g was reared in a box container with a capacity of 15 liters with a volume of 8 liters of water. Fish were kept for 30 days. The variables observed were length and height of the fish,carcass weight and protein content of fish meat. Environmental factors observed included dissolved oxygen (DO), pH, temperature, ammonia, nitrite and nitrate content. Data analysis was carried out using two way analysis of variance (ANOVA). Data analysis was carried out using the SPSS version 23 program. Significant differences between treatments were tested using Least Significant Difference test (LSD). Significant differences were evaluated at the level of P < 0.05. The results showed an increase in stocking density up to twice the normal capacity by using an aerator as a source of dissolved oxygen inhibiting growth and reducing carcass weight and protein content of fish meat. In this study it can be concluded that the stocking density of fish can still be increased up to twice the normal capacity with the addition of an aerator as an increase in ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114798275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prevalensi prediabetes meningkat setiap tahunnya dan diprediksi lebih dari 470 juta orang mengalami prediabetes pada tahun 2030. Induksi insulin eksogen secara terus-menerus menyebabkan resistensi insulin yang berujung pada kenaikan kadar glukosa darah yang dapat berefek pada histologi ren. Penelitian ini bertujuan untuk mengetahui gambaran histologi ren yang meliputi bobot ren, tebal kapsula Bowman, ukuran sel tubulus proksimal, ukuran sel tubulus distal, dan indeks ren setelah diinduksi dengan insulin eksogen. Penelitian ini dilaksanakan selama 6 bulan di Laboratorium Biologi Sktruktur dan Fungsi Hewan dan di kandang hewan uji, Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro. Penelitian ini menggunakan 21 ekor tikus putih yang dibagi menjadi 3 kelompok perlakuan dan 7 ulangan. P0 (kontrol), P1 (diiduksi insulin glargine 1,80 IU/200gBB/hari selama 14 hari), dan P2 (diinduksi insulin glargine 1,80 IU/200gBB/hari selama 28 hari). Insulin glargine diinjeksikan melalui subkutan dengan menggunakan xiulin pen. Preparat histologi ren dibuat dengan metode parafin dan pewarnaan Hematoksilin-Eosin. Pengamatan preparat menggunakan alat fotomikrograf. Data penelitian kemudian dianalisis dengan uji Anova. Hasil uji Anova menunjukkan antar perlakuan berbeda tidak nyata. Kesimpulan dari penelitian ini adalah induksi insulin 1,80 IU/200gBB/hari selama 14 hari dan 28 hari tidak berpotensi menyebabkan perubahan pada struktur histologi ren tikus putih. The prevalence of prediabetes increases every year and it is predicted that more than 470 million people will experience prediabetes in 2030. Continuous exogenous insulin induction causes insulin resistance which leads to an increase in blood glucose levels which can have an effect on renal histology. This study aims to determine the histology of the ren, which includes renal weight, Bowman's capsule thickness, proximal tubular cell size, distal tubular cell size, and ren index after being induced with exogenous insulin. This research was carried out for 6 months at the Laboratory of Structural and Functional Biology of Animals and in the cages of test animals, Department of Biology, Faculty of Science and Mathematics, Diponegoro University. This study used 21 white rats which were divided into 3 treatment groups and 7 replications. P0 (control), P1 (induced by insulin glargine 1.80 IU/200gBW/day for 14 days), and P2 (induced by insulin glargine 1.80 IU/200gBW/day for 28 days). Insulin glargine is injected subcutaneously using a xiulin pen. Renal histology preparations were made by paraffin method and Hematoxylin-Eosin staining. Observation of preparations using a photomicrograph. The research data were then analyzed by the Anova test. The results of the Anova test showed that the differences between treatments were not significantly different. The conclusion of this study was that insulin induction of 1.80 IU/200gBW/day for 14 days and 28 days did not have the potential to cause changes
{"title":"Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) yang Diinduksi Insulin.","authors":"Silvana Tana, Melia Nourmalitasari Shivaluhung, Teguh Suprihatin","doi":"10.14710/baf.7.2.2022.126-134","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.126-134","url":null,"abstract":"Prevalensi prediabetes meningkat setiap tahunnya dan diprediksi lebih dari 470 juta orang mengalami prediabetes pada tahun 2030. Induksi insulin eksogen secara terus-menerus menyebabkan resistensi insulin yang berujung pada kenaikan kadar glukosa darah yang dapat berefek pada histologi ren. Penelitian ini bertujuan untuk mengetahui gambaran histologi ren yang meliputi bobot ren, tebal kapsula Bowman, ukuran sel tubulus proksimal, ukuran sel tubulus distal, dan indeks ren setelah diinduksi dengan insulin eksogen. Penelitian ini dilaksanakan selama 6 bulan di Laboratorium Biologi Sktruktur dan Fungsi Hewan dan di kandang hewan uji, Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro. Penelitian ini menggunakan 21 ekor tikus putih yang dibagi menjadi 3 kelompok perlakuan dan 7 ulangan. P0 (kontrol), P1 (diiduksi insulin glargine 1,80 IU/200gBB/hari selama 14 hari), dan P2 (diinduksi insulin glargine 1,80 IU/200gBB/hari selama 28 hari). Insulin glargine diinjeksikan melalui subkutan dengan menggunakan xiulin pen. Preparat histologi ren dibuat dengan metode parafin dan pewarnaan Hematoksilin-Eosin. Pengamatan preparat menggunakan alat fotomikrograf. Data penelitian kemudian dianalisis dengan uji Anova. Hasil uji Anova menunjukkan antar perlakuan berbeda tidak nyata. Kesimpulan dari penelitian ini adalah induksi insulin 1,80 IU/200gBB/hari selama 14 hari dan 28 hari tidak berpotensi menyebabkan perubahan pada struktur histologi ren tikus putih. The prevalence of prediabetes increases every year and it is predicted that more than 470 million people will experience prediabetes in 2030. Continuous exogenous insulin induction causes insulin resistance which leads to an increase in blood glucose levels which can have an effect on renal histology. This study aims to determine the histology of the ren, which includes renal weight, Bowman's capsule thickness, proximal tubular cell size, distal tubular cell size, and ren index after being induced with exogenous insulin. This research was carried out for 6 months at the Laboratory of Structural and Functional Biology of Animals and in the cages of test animals, Department of Biology, Faculty of Science and Mathematics, Diponegoro University. This study used 21 white rats which were divided into 3 treatment groups and 7 replications. P0 (control), P1 (induced by insulin glargine 1.80 IU/200gBW/day for 14 days), and P2 (induced by insulin glargine 1.80 IU/200gBW/day for 28 days). Insulin glargine is injected subcutaneously using a xiulin pen. Renal histology preparations were made by paraffin method and Hematoxylin-Eosin staining. Observation of preparations using a photomicrograph. The research data were then analyzed by the Anova test. The results of the Anova test showed that the differences between treatments were not significantly different. The conclusion of this study was that insulin induction of 1.80 IU/200gBW/day for 14 days and 28 days did not have the potential to cause changes","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128473263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-27DOI: 10.14710/baf.7.2.2022.101-109
Aliza Shamita, Yulita Nurchayati, Nintya Setiari
Lahan pasir pantai kurang dimanfaatkan sebagai lahan petanian karena miskin hara dan tidak dapat mengikat air. Lahan pasir pantai dapat dioptimalkan dengan penggunaan pupuk organik dan anorganik. Penelitian ini mengkaji penggunaan pupuk organik berbahan dasar Azolla maupun kotoran sapi dibandingkan dengan pupuk anorganik NPK pada media pasir pantai terhadap pertumbuhan tanaman tomat varietas Servo. Bibit tomat umur 4 minggu dipindahkan ke dalam polybag yang berisi pasir pantai dengan perlakuan jenis pupuk. Desain penelitian menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal yaitu 3 jenis pupuk dengan perbandingan pasir:pupuk yaitu 2:1, berupa pupuk organik Azolla, pupuk organik kotoran sapi dan pupuk anorganik NPK (20% Nitrogen, 10% Fosfor, 10% Kalium) masing-masing sebanyak 5 ulangan. Parameter penelitian meliputi tinggi tanaman, panjang akar, bobot segar, bobot kering, diameter batang dan jumlah daun. Data hasil penelitian diuji statistik dengan Analysist of Varian (ANOVA), kemudian dilanjutkan uji Duncan Multiple Range Test (DMRT) tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa perlakuan jenis pupuk mempengaruhi pertumbuhan. Pupuk organik Azolla dan pupuk anorganik NPK meningkatkan diameter dan jumlah daun tanaman tomat, sedangkan pupuk organik berbahan dasar kotoran sapi meningkatkan panjang akar. Kesimpulan penelitian ini, pemberian pupuk organik Azolla dan pupuk anorganik NPK memberikan pengaruh lebih banyak terhadap pertumbuhan pada media pasir pantai. Beach sandland underutilized as agricultural because it is poor in nutrients and cannot bind water. Sandland can be optimized with the use of organic and inorganic fertilizers. This study examined the use of organic fertilizers based on Azolla and cow dung compared to NPK inorganic fertilizers in beach sand media to the growth of servo variety tomato plants. Tomato seedlings aged 4 weeks are transferred into polybags containing beach sand with fertilizer type treatment. The research design uses a complete random design (RAL) single factor, namely 3 types of fertilizers with sand comparison: fertilizers that are 2: 1, in the form of Azolla organic fertilizers, cow dung organic fertilizers and NPK inorganic fertilizers (20% Nitrogen, 10% Phosphorus, 10% Potassium) each as much as 5 repetitions. Research parameters include plant height, root length, fresh weight, dry weight, stem diameter and number of leaves. The data was tested statistically with the Analysist of Varian (ANOVA), followed by the Duncan Multiple Range Test (DMRT) with a confidence level of 95%. The results showed that the treatment of this type of fertilizer affects growth. Azolla organic fertilizer and NPK inorganic fertilizer increase the diameter and number of leaves of tomato plants, while organic fertilizers based on cow dung increase the length of roots. Conclusion of this study, the provision of Azolla organic fertilizer and NPK inorganic fertilizers exerts more influence on growth in beach sand media.
由于缺乏营养，沿海的沙丘被用作农民的土地被认为是低洼的，而且没有办法把水封闭起来。沿海沙滩可以优化有机和无机肥料。这项研究探讨了亚唑菌根和牛粪与沿海沙介质的非有机肥料对番茄淀粉品种生长的影响。4周大的番茄种子被转移到流沙中进行肥料处理。研究设计采用一种由三种肥料组成的单一随机设计(摩尔)来比较沙土:肥料为2:1，有机肥料为Azolla，有机肥料为牛粪，有机肥料为NPK(20%氮气，10%磷，10%钾)，每五种重复。研究参数包括植物的高度、根长、新鲜重量、干重量、茎的直径和叶子的数量。研究结果的数据通过变体分析(ANOVA)进行统计，然后继续测试邓肯的多程测试(DMRT) 95%的可靠性。研究表明，这种肥料的治疗会影响生长。有机肥料和无机肥料增加了番茄植物的直径和叶子的数量，而由牛粪制成的有机肥料增加了根部的长度。这项研究的结论是，向Azolla提供有机肥料和NPK的无机肥料对沿海沙质的生长产生了更大的影响。海滩没有农业用地，因为它缺乏营养，不能储水。桑兰德希望利用有机和非有机肥料。这项研究包括了基于Azolla的有机肥料的使用，并将奶牛分解成在沙滩和沙滩上生长的混合番茄。4周后，番茄将被转移到多包接触沙滩和肥料类型的土壤中。研究设计利用a完全随机设计杂志》(财富)单因子,namely 3 types of fertilizers with沙不那么可怜:fertilizers那是2:1,in The form of Azolla有机fertilizers,牛宁录有机fertilizers和NPK inorganic fertilizers(氮,磷,钾10%)10% 20%每5 repetitions一样多。研究parameters包括plant height, root length, fresh weight, dry weight, stem直径和叶数。数据是由邓肯的多数性测试所遵循的对变量的分析结果的结果，其可靠性是95%。推荐人表示，这种fertilizer的影响正在增长。有机有机肥料和非有机肥料增加了植物番茄的直径和数量，而有机植物的根系增加了根的粘结剂。这项研究的结论，对Azolla有机肥料和npa的介绍，对沙滩媒体的增长有更多的过度影响。
{"title":"Respon Pertumbuhan Tanaman Tomat (Lycopersicon esculentum Mill.) yang diberi Perlakuan Jenis Pupuk Organik dan Anorganik pada Media Pasir Pantai","authors":"Aliza Shamita, Yulita Nurchayati, Nintya Setiari","doi":"10.14710/baf.7.2.2022.101-109","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.101-109","url":null,"abstract":"Lahan pasir pantai kurang dimanfaatkan sebagai lahan petanian karena miskin hara dan tidak dapat mengikat air. Lahan pasir pantai dapat dioptimalkan dengan penggunaan pupuk organik dan anorganik. Penelitian ini mengkaji penggunaan pupuk organik berbahan dasar Azolla maupun kotoran sapi dibandingkan dengan pupuk anorganik NPK pada media pasir pantai terhadap pertumbuhan tanaman tomat varietas Servo. Bibit tomat umur 4 minggu dipindahkan ke dalam polybag yang berisi pasir pantai dengan perlakuan jenis pupuk. Desain penelitian menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal yaitu 3 jenis pupuk dengan perbandingan pasir:pupuk yaitu 2:1, berupa pupuk organik Azolla, pupuk organik kotoran sapi dan pupuk anorganik NPK (20% Nitrogen, 10% Fosfor, 10% Kalium) masing-masing sebanyak 5 ulangan. Parameter penelitian meliputi tinggi tanaman, panjang akar, bobot segar, bobot kering, diameter batang dan jumlah daun. Data hasil penelitian diuji statistik dengan Analysist of Varian (ANOVA), kemudian dilanjutkan uji Duncan Multiple Range Test (DMRT) tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa perlakuan jenis pupuk mempengaruhi pertumbuhan. Pupuk organik Azolla dan pupuk anorganik NPK meningkatkan diameter dan jumlah daun tanaman tomat, sedangkan pupuk organik berbahan dasar kotoran sapi meningkatkan panjang akar. Kesimpulan penelitian ini, pemberian pupuk organik Azolla dan pupuk anorganik NPK memberikan pengaruh lebih banyak terhadap pertumbuhan pada media pasir pantai. Beach sandland underutilized as agricultural because it is poor in nutrients and cannot bind water. Sandland can be optimized with the use of organic and inorganic fertilizers. This study examined the use of organic fertilizers based on Azolla and cow dung compared to NPK inorganic fertilizers in beach sand media to the growth of servo variety tomato plants. Tomato seedlings aged 4 weeks are transferred into polybags containing beach sand with fertilizer type treatment. The research design uses a complete random design (RAL) single factor, namely 3 types of fertilizers with sand comparison: fertilizers that are 2: 1, in the form of Azolla organic fertilizers, cow dung organic fertilizers and NPK inorganic fertilizers (20% Nitrogen, 10% Phosphorus, 10% Potassium) each as much as 5 repetitions. Research parameters include plant height, root length, fresh weight, dry weight, stem diameter and number of leaves. The data was tested statistically with the Analysist of Varian (ANOVA), followed by the Duncan Multiple Range Test (DMRT) with a confidence level of 95%. The results showed that the treatment of this type of fertilizer affects growth. Azolla organic fertilizer and NPK inorganic fertilizer increase the diameter and number of leaves of tomato plants, while organic fertilizers based on cow dung increase the length of roots. Conclusion of this study, the provision of Azolla organic fertilizer and NPK inorganic fertilizers exerts more influence on growth in beach sand media.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116079744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-27DOI: 10.14710/baf.7.2.2022.110-119
Sri Isdadiyanto, Anggih Retno Pratiwi, Siti Muflichatun Mardiati
Pakan tinggi lemak terutama kolesterol dapat menyebabkan terjadinya hiperlipidemia, hal ini merupakan salah satu faktor pemicu terjadinya penyakit perlemakan hepar non alkoholik. Azadirachta indica A. Juss (Mimba) merupakan tanaman herbal yang mengandung antioksidan, antihiperlipidemia, dan memiliki aktivitas hepatoprotektor. Penelitian ini bertujuan untuk mengetahui pengaruh paparan ekstrak etanol daun mimba (Azadirachta indica A. Juss) terhadap hepatosomatic index (HSI) tikus putih (Rattus norvegicus L.) yang diberi diet tinggi lemak. Dua puluh empat ekor tikus putih dibagi menjadi 6 kelompok perlakuan yaitu, P0 = kontrol normal, P1= kontrol negatif (diet tinggi lemak), P2 (diet tinggi lemak+simvastatin), P3 (diet tinggi lemak + dosis 75mg/ 200gBB), P4 (diet tinggi lemak+dosis 100mg/ 200gBB) dan P5 (diet tinggi lemak+ 125mg/ 200gBB). Penentuan HSI diperoleh dari bobot hepar dan bobot badan. Preparat dibuat dengan metode paraffin dan pewarnaan HE. Data berat badan, berat hepar dan hepatosomatic index yang homogen dan berdistribusi normal dianalisis secara statistik menggunakan ANOVA dilanjutkan dengan uji Duncan dengan taraf signifikansi 5% menggunakan software SPSS 16.0. Hasil analisis hepatosomatic index pada kelompok perlakuan menunjukkan tidak berbeda nyata (p>0,05). Sehingga dapat disimpulkan bahwa pemberian ekstrak etanol daun mimba tergolong aman untuk digunakan sebagai obat herbal. Diets high in fat, especially cholesterol, can cause hyperlipidemia, this is one of the triggering factors for non-alcoholic fatty liver disease. Azadirachta indica A. Juss (Neem) is an herbal plant that contains antioxidants, antihyperlipidemia, and has hepatoprotective activity. This study aims to determine the effect of exposure to ethanolic extract of neem leaves (Azadirachta indica A. Juss) on the hepatosomatic index (HSI) of white rats (Rattus norvegicus L.) fed a high-fat diet. Twenty-four white rats were divided into 6 treatment groups, namely, P0 = normal control, P1 = negative control (high fat diet), P2 (high fat diet + simvastatin), P3 (high fat diet + 75mg/200gBW dose), P4 (high-fat diet + dose of 100mg/200gBW) and P5 (high-fat diet+125mg/200gBW). Determination of HSI was obtained from liver weight and body weight. Data on body weight, liver weight and hepatosomatic index which were homogeneous and normally distributed were statistically analyzed using ANOVA followed by Duncan's test with a significance level of 5% using SPSS 16.0 software. The results of the analysis of the hepatosomatic index in the treatment group showed no significant difference (p>0.05). So it can be concluded that the administration of neem leaf ethanol extract is safe for use as herbal medicine.
{"title":"Indeks Hepatosomatic Rattus norvegicus Hiperlipidemia Setelah Paparan Ekstrak Etanol Daun Azadirachta Indica","authors":"Sri Isdadiyanto, Anggih Retno Pratiwi, Siti Muflichatun Mardiati","doi":"10.14710/baf.7.2.2022.110-119","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.110-119","url":null,"abstract":"Pakan tinggi lemak terutama kolesterol dapat menyebabkan terjadinya hiperlipidemia, hal ini merupakan salah satu faktor pemicu terjadinya penyakit perlemakan hepar non alkoholik. Azadirachta indica A. Juss (Mimba) merupakan tanaman herbal yang mengandung antioksidan, antihiperlipidemia, dan memiliki aktivitas hepatoprotektor. Penelitian ini bertujuan untuk mengetahui pengaruh paparan ekstrak etanol daun mimba (Azadirachta indica A. Juss) terhadap hepatosomatic index (HSI) tikus putih (Rattus norvegicus L.) yang diberi diet tinggi lemak. Dua puluh empat ekor tikus putih dibagi menjadi 6 kelompok perlakuan yaitu, P0 = kontrol normal, P1= kontrol negatif (diet tinggi lemak), P2 (diet tinggi lemak+simvastatin), P3 (diet tinggi lemak + dosis 75mg/ 200gBB), P4 (diet tinggi lemak+dosis 100mg/ 200gBB) dan P5 (diet tinggi lemak+ 125mg/ 200gBB). Penentuan HSI diperoleh dari bobot hepar dan bobot badan. Preparat dibuat dengan metode paraffin dan pewarnaan HE. Data berat badan, berat hepar dan hepatosomatic index yang homogen dan berdistribusi normal dianalisis secara statistik menggunakan ANOVA dilanjutkan dengan uji Duncan dengan taraf signifikansi 5% menggunakan software SPSS 16.0. Hasil analisis hepatosomatic index pada kelompok perlakuan menunjukkan tidak berbeda nyata (p>0,05). Sehingga dapat disimpulkan bahwa pemberian ekstrak etanol daun mimba tergolong aman untuk digunakan sebagai obat herbal. Diets high in fat, especially cholesterol, can cause hyperlipidemia, this is one of the triggering factors for non-alcoholic fatty liver disease. Azadirachta indica A. Juss (Neem) is an herbal plant that contains antioxidants, antihyperlipidemia, and has hepatoprotective activity. This study aims to determine the effect of exposure to ethanolic extract of neem leaves (Azadirachta indica A. Juss) on the hepatosomatic index (HSI) of white rats (Rattus norvegicus L.) fed a high-fat diet. Twenty-four white rats were divided into 6 treatment groups, namely, P0 = normal control, P1 = negative control (high fat diet), P2 (high fat diet + simvastatin), P3 (high fat diet + 75mg/200gBW dose), P4 (high-fat diet + dose of 100mg/200gBW) and P5 (high-fat diet+125mg/200gBW). Determination of HSI was obtained from liver weight and body weight. Data on body weight, liver weight and hepatosomatic index which were homogeneous and normally distributed were statistically analyzed using ANOVA followed by Duncan's test with a significance level of 5% using SPSS 16.0 software. The results of the analysis of the hepatosomatic index in the treatment group showed no significant difference (p>0.05). So it can be concluded that the administration of neem leaf ethanol extract is safe for use as herbal medicine.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134088373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-26DOI: 10.14710/baf.7.2.2022.91-100
Antika Febiola Utami, Sutyarso Sutyarso, Sri Wahyuningsih, Nuning Nurcahyani
Hiperglikemia terjadi ketika keadaan kadar glukosa darah melebihi batas normal. Daun pepaya (Carica papaya L.) dan daun kemangi (Ocimum x africanum Lour.) dilaporkan mengandung senyawa yang berperan sebagai antihiperglikemia. Penelitian ini dilakukan karena dari beberapa khasus di Indonesia, penderita hiperglikemia cukup tinggi maka penelitian ini perlu dilakukan untuk memberikan informasi mengenai efektivias dari suatu tanaman herbal dalam menangai hiperglikemia.Tujuan penelitian untuk mengetahui perbandingan efektivitas pemberian ekstrak etanol daun pepaya dan daun kemangi dalam menurunkan kadar glukosa darah mencit hiperglikemia. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 25 ekor mencit dibagi dalam 5 kelompok perlakuan dan 5 kali ulangan. Kelompok perlakuan diantaranya, K(N) (tanpa perlakuan), K(-) (diinduksi aloksan ), K(+) (diinduksi aloksan dan diberi perlakuan dengan glibenklamid), P1 perlakuan 1 (diinduksi aloksan dan diberi perlakuan dengan ekstrak etanol daun pepaya), P2 perlakuan 2 (diinduksi aloksan dan diberi perlakuan dengan ekstrak etanol daun kemangi). Parameter yang diukur dalam penelitian ini yaitu berat badan dan kadar glukosa darah mencit. Data yang diperoleh dianalisis dengan one way ANOVA dan uji lanjut LSD pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian bahan uji mampu menurunkan kadar glukosa darah mencit dengan persentase penurunan lebih efektif pada P2 sebesar 60,15% sedangkan P1 sebesar 53,32% . Hyperglycemia occurs when blood glucose levels exceed normal limits. Papaya (Carica papaya L.) and basil (Ocimum x africanum Lour.) leaves are reported to contain compounds that act as antihyperglycemic agents. This study was conducted because in some cases in Indonesia, people with hyperglycemia are quite high, so this study needs to be carried out to provide information about the effectiveness of an herbal plant in dealing with hyperglycemia. hyperglycemic mice blood. This study used a completely randomized design (CRD) with 25 mice divided into 5 treatment groups and 5 replications. The treatment groups included, K(N) (without treatment), K(-) (alloxan induced), K(+) (alloxan induced and treated with glibenclamide), P1 treatment 1 (alloxan induced and treated with ethanol extract of papaya leaves ), P2 treatment 2 (alloxan induced and treated with ethanol extract of basil leaves). The parameters measured in this study were body weight and blood glucose levels of mice. The data obtained were analyzed by one way ANOVA and LSD further test at the 5% level. The results showed that the administration of the test material was able to reduce blood glucose levels in mice with a more effective percentage reduction in P2 of 60.15% while P1 of 53.32%.
高血糖发生在血糖水平高于正常水平的情况下。据报道，木瓜叶(Carica papaya L.)和罗勒叶(Ocimum x africanum Lour)含有一种抗血糖的化合物。这项研究的原因是，在印度尼西亚，高血糖症患者的数量很高，因此有必要提供有关草药在高血糖标记中所起作用的信息。研究的目的是比较木瓜叶和罗勒提取物以乙醇为原料的效力，以降低虎皮高血糖水平。本研究采用全随机设计(摩尔)，25只小数被分成5个治疗组和5次重复。这些治疗包括K(N)(不治疗)，K(-)在这项研究中测量的参数是体重和葡萄糖水平。所获得的数据经过一种方式的分析，并在5%的迷幻药试验中进行测试。研究表明，试验药物的作用是通过P2的降低率降低60.15%，而P1的降低率为53.32%。当血液葡萄糖水平超出正常极限时，高血糖就会发生。木瓜(Carica Papaya L)和罗勒(Ocimum x africanum Lour)报道了美国反草甘膦代理的赔偿。这项研究是受委托的，因为在印尼的某些cases中，高尿酸症的人非常高，所以这项研究需要提供有关一种草药在处理高尿酸症时的效果的信息。高血糖。这个研究用了一个完整的分级设计，有25个mice分解成5个treatgroups和5个复制品。treatment groups包括K(N)、K(-)(异步诱导)、K(+)(异步诱导和治疗与glibenclamide)、P1 treatment 1(异步诱导和治疗与木瓜叶乙醇extract)、P2 treatment 2(异步诱导和treatment extract with bacbaral extract)。parameters在这项研究中提出了身体重量和血液葡萄糖水平。我们在5%的测试中对异常和LSD进行了分析。结果显示，材料测试的行政部门可以在比方说更有效的抑制率为60% .15%
{"title":"Perbandingan Efektivitas Ekstrak Etanol Daun Pepaya (Carica papaya L.) dan Ekstrak Etanol Daun Kemangi (Ocimum x africanum Lour.) dalam Menurunkan Kadar Glukosa Darah Mencit (Mus musculus L.) Hiperglikemia","authors":"Antika Febiola Utami, Sutyarso Sutyarso, Sri Wahyuningsih, Nuning Nurcahyani","doi":"10.14710/baf.7.2.2022.91-100","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.91-100","url":null,"abstract":"Hiperglikemia terjadi ketika keadaan kadar glukosa darah melebihi batas normal. Daun pepaya (Carica papaya L.) dan daun kemangi (Ocimum x africanum Lour.) dilaporkan mengandung senyawa yang berperan sebagai antihiperglikemia. Penelitian ini dilakukan karena dari beberapa khasus di Indonesia, penderita hiperglikemia cukup tinggi maka penelitian ini perlu dilakukan untuk memberikan informasi mengenai efektivias dari suatu tanaman herbal dalam menangai hiperglikemia.Tujuan penelitian untuk mengetahui perbandingan efektivitas pemberian ekstrak etanol daun pepaya dan daun kemangi dalam menurunkan kadar glukosa darah mencit hiperglikemia. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 25 ekor mencit dibagi dalam 5 kelompok perlakuan dan 5 kali ulangan. Kelompok perlakuan diantaranya, K(N) (tanpa perlakuan), K(-) (diinduksi aloksan ), K(+) (diinduksi aloksan dan diberi perlakuan dengan glibenklamid), P1 perlakuan 1 (diinduksi aloksan dan diberi perlakuan dengan ekstrak etanol daun pepaya), P2 perlakuan 2 (diinduksi aloksan dan diberi perlakuan dengan ekstrak etanol daun kemangi). Parameter yang diukur dalam penelitian ini yaitu berat badan dan kadar glukosa darah mencit. Data yang diperoleh dianalisis dengan one way ANOVA dan uji lanjut LSD pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian bahan uji mampu menurunkan kadar glukosa darah mencit dengan persentase penurunan lebih efektif pada P2 sebesar 60,15% sedangkan P1 sebesar 53,32% . Hyperglycemia occurs when blood glucose levels exceed normal limits. Papaya (Carica papaya L.) and basil (Ocimum x africanum Lour.) leaves are reported to contain compounds that act as antihyperglycemic agents. This study was conducted because in some cases in Indonesia, people with hyperglycemia are quite high, so this study needs to be carried out to provide information about the effectiveness of an herbal plant in dealing with hyperglycemia. hyperglycemic mice blood. This study used a completely randomized design (CRD) with 25 mice divided into 5 treatment groups and 5 replications. The treatment groups included, K(N) (without treatment), K(-) (alloxan induced), K(+) (alloxan induced and treated with glibenclamide), P1 treatment 1 (alloxan induced and treated with ethanol extract of papaya leaves ), P2 treatment 2 (alloxan induced and treated with ethanol extract of basil leaves). The parameters measured in this study were body weight and blood glucose levels of mice. The data obtained were analyzed by one way ANOVA and LSD further test at the 5% level. The results showed that the administration of the test material was able to reduce blood glucose levels in mice with a more effective percentage reduction in P2 of 60.15% while P1 of 53.32%. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115011916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.14710/baf.7.2.2022.83-90
N. Sofiyanti, Dyah Iriani, Asri Ria Lestari
Tanaman pucuk merah (Syzygium myrtifolium Walp.) merupakan salah satu tanaman yang termasuk dalam famili Myrtaceae. Penelitian ini bertujuan melakukan kajian anatomi-histokimia petiole dan karakteristik epidermis daun pucuk merah. Sampel yang diambil adalah daun pucuk merah yang sudah dewasa. Penelitian ini dilakukan pada bulan April sampai Juni 2022. Pembuatan preparat anatomi dilakukan dengan metode parafin, sedangkan uji histokimia dilakukan pada senyawa alkaloid, tanin, flavonoid, dan lipid dengan metode kualitatif. Pengamatan karakteristik epidermis dilakukan dengan metode paradermal. Hasil pengamatan anatomi penampang melintang petiole pucuk merah menunjukan bentuk agak oval, membulat pada bagian dorsal dan bersayap pada bagian ventral. Kutikula terdapat di sebelah luar epidermis. Korteks terdiri dari lapisan hipodermis dan parenkim. Hipodermis tersusun dari 4 – 5 lapisan sel-sel kolenkim memanjang, dan terdapat rongga sekresi berisi minyak. Parenkim tersusun dari 17 – 19 sel sel poligonal. Berkas pembuluh berada di tengah dengan tipe bikolateral. Hasil uji histokimia petiole menunjukkan hasil positif flavonoid, alkaloid, tanin, dan lipid. Stomata pucuk merah termasuk hipostomatik dengan tipe parasitik. Sel-sel epidermis poligonal dengan dinding sel bergelombang (undulate). Hasil kajian ini memberikan informasi tambahan mengenai anatomi, histokimia dari petiolus pucuk merah serta memberikan informasi karakteristik epidermis dan stomata tanaman ini. The red tip plant (Syzygium myrtifolium Walp.) belongs to Myrtaceae family. The aim of this study was to investigate the anatomical-histochemical of ptiole as well as epidermal chrakteristic of red tip plant. Leaf samples were collected sixth node from the top. This study had been conducted from April to June 2022. The anatomical preparation used paraffine method. Histochemistry test was carried out for alkaloid, tannin, flavonoid and lipid using qualitative method. Epidermal charateristic was observed using paradermal method. The anatomical result shows that the cross section of petiole of red tip plant is slightly ovale-shaped, with rounded part at dorsal side dan winged-part at ventral side. The outermost part is cuticle layer. Cortex is consisted of hipoderm and parenchyma layers. Hipoderm is arranged by 4 to 5 layers of elongated cholencym cells, with secretion structure or oil gland. Parenchym is consisted of 17 – 19 layers of polygonal cells. Vascular bundle is located in the center, with bicholateral type. The histochemistry test indicated positive content of flavonoid, alkaloid, tannin and lipid. The stomata of red tip plant is hypostomatic with paracytic type. The epiderm cells are polygonal with undulate cell walls.The result of this study provide the additional information of anatomy, histochemistry of petiole of red tip land, and also provide the information of epidermal characteristic of this plant.
红斑植物(Syzygium myrtifolium Walp)是属于Myrtaceae家族的一种植物。这项研究的目的是对红斑叶皮上皮的解剖组织和表皮特征进行研究。采集的样本是成熟的红斑叶。这项研究是在2022年4月至6月进行的。解剖学制剂等方法进行制作石蜡进行测试,而histokimia单宁,生物碱类黄酮化合物,和脂肪定性的方法。表皮特征paradermal方法进行观察。petiole封剖面图的解剖观察结果显示红色有点椭圆形的形状,圆在腹侧背和固定翼的部分。表皮角质层见于在外面。实质由皮下层和皮层中。皮下的4—5层kolenkim伸长细胞组成,有分泌物包含油腔。Parenkim 17—19细胞细胞呈多边形组成。文件在中间的那种bikolateral血管。化验结果histokimia petiole表示阳性结果类黄酮、生物碱,单宁和脂肪。气孔封包括hipostomatik的红型为基础。多边形的表皮细胞的细胞壁起伏(undulate)。这些研究结果提供了更多关于解剖学,histokimia信息从红petiolus封,并提供了这种植物的表皮和气孔特征信息。红小费普兰特(Syzygium myrtifolium Walp)属于Myrtaceae家庭。aim of this study是to investigate ptiole anatomical-histochemical》红的表皮as well as chrakteristic普兰小费。叶样本是collected第六节点从《top。这个研究had been conducted 2022年从四月到六月。《anatomical准备过去paraffine方法。Histochemistry测试是carried out for生物碱,tannin qqe,类黄酮和脂肪利用方法。表皮charateristic是用paradermal observed方法。十字区》《anatomical论点节目那petiole红小费的植物是有点ovale-shaped里,用rounded部分at背侧和腹侧winged-part at一边。The outermost是cuticle层层部分。大脑皮层是consisted hipoderm parenchyma著作百科全书》雀跃。Hipoderm是安排由4到5雀跃的瘦长的cholencym细胞里,用secretion gland的vesalius或石油。Parenchym是consisted 17—19 polygonal之雀跃的细胞。实际上是中心的定位》里,用非堆bicholateral型。《histochemistry indicated阳性测试内容的类黄酮,生物碱,tannin和脂肪。《红小费植物气孔是和paracytic hypostomatic型。沃尔斯和undulate epiderm细胞是polygonal细胞。这个论点》研究资讯网措施》。《实习医生格蕾》红小费histochemistry of petiole of land,表皮和资讯网》也。characteristic of这个工厂。
{"title":"Kajian Anatomi-Histokimia Tangkai Daun dan Karakteristik Epidermis Pucuk Merah (Syzygium myrtifolium Walpp. – Myrtaceae)","authors":"N. Sofiyanti, Dyah Iriani, Asri Ria Lestari","doi":"10.14710/baf.7.2.2022.83-90","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.83-90","url":null,"abstract":"Tanaman pucuk merah (Syzygium myrtifolium Walp.) merupakan salah satu tanaman yang termasuk dalam famili Myrtaceae. Penelitian ini bertujuan melakukan kajian anatomi-histokimia petiole dan karakteristik epidermis daun pucuk merah. Sampel yang diambil adalah daun pucuk merah yang sudah dewasa. Penelitian ini dilakukan pada bulan April sampai Juni 2022. Pembuatan preparat anatomi dilakukan dengan metode parafin, sedangkan uji histokimia dilakukan pada senyawa alkaloid, tanin, flavonoid, dan lipid dengan metode kualitatif. Pengamatan karakteristik epidermis dilakukan dengan metode paradermal. Hasil pengamatan anatomi penampang melintang petiole pucuk merah menunjukan bentuk agak oval, membulat pada bagian dorsal dan bersayap pada bagian ventral. Kutikula terdapat di sebelah luar epidermis. Korteks terdiri dari lapisan hipodermis dan parenkim. Hipodermis tersusun dari 4 – 5 lapisan sel-sel kolenkim memanjang, dan terdapat rongga sekresi berisi minyak. Parenkim tersusun dari 17 – 19 sel sel poligonal. Berkas pembuluh berada di tengah dengan tipe bikolateral. Hasil uji histokimia petiole menunjukkan hasil positif flavonoid, alkaloid, tanin, dan lipid. Stomata pucuk merah termasuk hipostomatik dengan tipe parasitik. Sel-sel epidermis poligonal dengan dinding sel bergelombang (undulate). Hasil kajian ini memberikan informasi tambahan mengenai anatomi, histokimia dari petiolus pucuk merah serta memberikan informasi karakteristik epidermis dan stomata tanaman ini. The red tip plant (Syzygium myrtifolium Walp.) belongs to Myrtaceae family. The aim of this study was to investigate the anatomical-histochemical of ptiole as well as epidermal chrakteristic of red tip plant. Leaf samples were collected sixth node from the top. This study had been conducted from April to June 2022. The anatomical preparation used paraffine method. Histochemistry test was carried out for alkaloid, tannin, flavonoid and lipid using qualitative method. Epidermal charateristic was observed using paradermal method. The anatomical result shows that the cross section of petiole of red tip plant is slightly ovale-shaped, with rounded part at dorsal side dan winged-part at ventral side. The outermost part is cuticle layer. Cortex is consisted of hipoderm and parenchyma layers. Hipoderm is arranged by 4 to 5 layers of elongated cholencym cells, with secretion structure or oil gland. Parenchym is consisted of 17 – 19 layers of polygonal cells. Vascular bundle is located in the center, with bicholateral type. The histochemistry test indicated positive content of flavonoid, alkaloid, tannin and lipid. The stomata of red tip plant is hypostomatic with paracytic type. The epiderm cells are polygonal with undulate cell walls.The result of this study provide the additional information of anatomy, histochemistry of petiole of red tip land, and also provide the information of epidermal characteristic of this plant.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"220 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115652022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-24DOI: 10.14710/baf.7.2.2022.66-74
Pierre Charismanuel Gideon, S. Sucahyo, S. P. Hastuti
Salah satu pakan alami yang paling baik dalam budidaya perikanan adalah D. magna yang sudah banyak dibudidayakan dan dapat diambil langsung dari alam, namun belum cukup dalam memenuhi kebutuhan. Untuk itu penelitian ini bertujuan untuk mengetahui pengaruh pertumbuhan populasi dan kualitas warna dari D. magna berdasarkan lama aerasi sehingga dapat mengetahui lama aerasi yang paling optimal untuk kultur D. magna. Penelitian ini dilaksanakan dengan membuat tiga faktor perlakuan dan satu faktor kontrol. Faktor kontrol adalah tanpa aerasi, faktor A dengan aerasi 3 jam/hari, faktor B dengan aerasi 6 jam/hari, dan faktor C dengan 24 jam aerasi. Masing – masing faktor memiliki 3 kali pengulangan. Hasil penelitian menunjukkan bahwa lama waktu aerasi 3 jam/hari adalah faktor paling optimal dalam menunjang pertumbuhan populasi D. magna dan faktor kontrol yaitu tanpa pemberian aerasi paling optimal dalam menunjang warna D. magna. Hasil analisis data dalam uji Friedman menunjukkan adanya perbedaan pengaruh pada pertumbuhan D. magna. Hasil pengukuran kualitas air menunjukan kadar DO cenderung turun bahkan melewati batas minimum, namun pada faktor C kadar DO masih dalam kadar optimal. Suhu berkisar 24.1 – 30.6 ℃ dan pH berkisar 6.3 – 7.0. Kondisi ini masih dalam kisaran optimum untuk kultur D. magna. One of the best natural feeds in aquaculture is D. magna which has been widely cultivated and can be taken directly from nature, but is not sufficient to meet the needs. For this reason, this study aims to determine the effect of population growth and color quality of D. magna based on aeration time so that can determine the most optimal aeration time for D. magna culture. This research was conducted by making three treatment factors and one control factor. The control factor was without aeration, factor A with aeration 3 hours/day, factor B with aeration 6 hours/day, and factor C with 24 hours aeration respectively. Each factor has 3 repetitions. The results showed that aeration time of 3 hours/day was the most optimal factor in supporting the growth of D. magna population and the control factor without aeration was the most optimal in supporting the color of D. magna. The results of data analysis in the Friedman test showed there were different effect on the growth of D. magna. The results of water quality measurements show that DO levels tend to fall even past the minimum limit, but in factor C, DO levels are still in optimal levels. The measurement of temperature ranges from 24.1 – 30.6 and the pH ranges from 6.3 – 7.0. This condition was still in the optimum range for D. magna culture
渔业养殖中最出色的自然饲料之一是D. magna，它已经被广泛种植，可以直接从自然中提取，但还不足以满足需求。为此,本研究旨在探讨影响人口增长和质量D .大的颜色根据曝气,以便知道久大文化的最优D曝气。研究这三个因素和待遇一个完美执行的控制因素。控制因素是无曝气，A是3小时/天曝气，B是6小时/天曝气，C是24小时曝气。每个因子有3次重复。研究结果表明,3小时-曝气时间是一天中最理想的因素促进人口增长D。麦格纳和控制因素,即没有礼物大维持D颜色中最理想的曝气。弗里德曼测试的数据分析显示，D. magna增长的影响有所不同。水质量的测量表明DO水平下降甚至超过了最低限度，但是C级DO仍然处于最佳水平。24 . 1—30不等。6℃温度和pH值范围6 3—7。0。这种情况还在为最佳范围大文化D。一号》最佳自然助长在aquaculture是D。麦格纳,这已被widely cultivated和可以成为了直接来自《自然》(nature),但is not sufficient高兴见到的需要。为了这个原因,这个效应》study aims to个重大的人口增长和颜色品质D .大的改编自aeration最佳的时间所以那能个重大最aeration时间D大文化。这个研究是conducted一号由制造三个治疗factors和控制因素。控制因子是没有aeration因子和aeration 3小时- A day,因子B aeration 6小时- day,和因子C和24小时aeration respectively。每因子3 repetitions了。results那里那个时代aeration》3小时- day是最理想因子》supporting D .大的人口增长》和《最优控制因子没有aeration是在大supporting不朽之[D。弗里德曼境results of数据分析测试那里有些不同的效应》D .大的增长。水质量艾默生秀那DO水平之results tend跌倒甚至过去最低限额,但在因子C仍然是在最佳水平,做水平。《ranges的温度测量从24 1—30。6和《pH ranges从3—7。0。这雾是大文化还是《最佳射程为D。
{"title":"Pengaruh Lama Aerasi yang Berbeda Terhadap Pertumbuhan Populasi dan Kualitas Warna dari Kutu Air Raksasa (Daphnia magna)","authors":"Pierre Charismanuel Gideon, S. Sucahyo, S. P. Hastuti","doi":"10.14710/baf.7.2.2022.66-74","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.66-74","url":null,"abstract":"Salah satu pakan alami yang paling baik dalam budidaya perikanan adalah D. magna yang sudah banyak dibudidayakan dan dapat diambil langsung dari alam, namun belum cukup dalam memenuhi kebutuhan. Untuk itu penelitian ini bertujuan untuk mengetahui pengaruh pertumbuhan populasi dan kualitas warna dari D. magna berdasarkan lama aerasi sehingga dapat mengetahui lama aerasi yang paling optimal untuk kultur D. magna. Penelitian ini dilaksanakan dengan membuat tiga faktor perlakuan dan satu faktor kontrol. Faktor kontrol adalah tanpa aerasi, faktor A dengan aerasi 3 jam/hari, faktor B dengan aerasi 6 jam/hari, dan faktor C dengan 24 jam aerasi. Masing – masing faktor memiliki 3 kali pengulangan. Hasil penelitian menunjukkan bahwa lama waktu aerasi 3 jam/hari adalah faktor paling optimal dalam menunjang pertumbuhan populasi D. magna dan faktor kontrol yaitu tanpa pemberian aerasi paling optimal dalam menunjang warna D. magna. Hasil analisis data dalam uji Friedman menunjukkan adanya perbedaan pengaruh pada pertumbuhan D. magna. Hasil pengukuran kualitas air menunjukan kadar DO cenderung turun bahkan melewati batas minimum, namun pada faktor C kadar DO masih dalam kadar optimal. Suhu berkisar 24.1 – 30.6 ℃ dan pH berkisar 6.3 – 7.0. Kondisi ini masih dalam kisaran optimum untuk kultur D. magna. One of the best natural feeds in aquaculture is D. magna which has been widely cultivated and can be taken directly from nature, but is not sufficient to meet the needs. For this reason, this study aims to determine the effect of population growth and color quality of D. magna based on aeration time so that can determine the most optimal aeration time for D. magna culture. This research was conducted by making three treatment factors and one control factor. The control factor was without aeration, factor A with aeration 3 hours/day, factor B with aeration 6 hours/day, and factor C with 24 hours aeration respectively. Each factor has 3 repetitions. The results showed that aeration time of 3 hours/day was the most optimal factor in supporting the growth of D. magna population and the control factor without aeration was the most optimal in supporting the color of D. magna. The results of data analysis in the Friedman test showed there were different effect on the growth of D. magna. The results of water quality measurements show that DO levels tend to fall even past the minimum limit, but in factor C, DO levels are still in optimal levels. The measurement of temperature ranges from 24.1 – 30.6 and the pH ranges from 6.3 – 7.0. This condition was still in the optimum range for D. magna culture","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125467699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rokok elektrik (RE) merupakan inovasi terbaru rokok yaitu tanpa proses pembakaran tar dan tembakau dan dianggap lebih aman dibandingkan rokok konvensional. Penelitian ini bertujuan mengetahui pengaruh paparan aerosol RE terhadap profil hematologis tikus putih (Rattus norvegicus) yang meliputi kadar hemoglobin, eritrosit, leukosit serta jenis leukosit. Dalam penelitian ini hewan uji Rattus norvegicus dibagi menjadi 4 kelompok dengan perlakuan yang berbeda yaitu diberi 0x paparan/hari (kontrol); 1x paparan/hari, 2x paparan/hari, dan 3x paparan/hari selama 14 hari. Setiap paparan dilakukan selama 5 menit (10 kali hembusan). Sampel darah diambil pada hari ke-0, hari ke-7 dan ke-14 melalui sinus orbitalis. Jumlah eritrosit dan leukosit dihitung dengan metode hitung bilik Neubauer. Kadar hemoglobin ditentukan menggunakan metode Sahli, dan persentase jenis leukosit dihitung dari preparat apus darah dengan pewarnaan Giemsa. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan terjadi peningkatan kadar hemoglobin dan jumlah eritrosit yang sejalan dengan peningkatan frekuensi dan durasi paparan RE sebagai kompensasi dari kondisi hipoksia. Jumlah leukosit juga cenderung meningkat, tetapi peningkatannya tidak linier dengan peningkatan frekuensi dan durasi paparan RE. Persentase limfosit, monosit dan eosinofil dibandingkan kontrol cenderung meningkat sedangkan persentase neutrofil cenderung menurun. Dengan demikian paparan RE dapat mempengaruhi profil hematologis hewan uji. E-cigarettes are the latest innovations of cigarettes without process of burning tar and tobacco. They are considered safer than conventional cigarettes. The purpose of this study was to determine the effect of e-cigarette aerosol exposure on the hematological profiles of Wistar rats (Rattus norvegicus). Rats were exposed to e-cigarette aerosol in different frequencies: 0x (control); once; twice; and 3x exposure/day. The experiment was ended on day 14th. Blood samples were taken through the orbital sinus on the day 0, 7th and 14th. Erythrocyte and leucocyte numbers were measured in Neubauer chamber count. Hemoglobin levels was determined by using Sahli method, and the percentage of leukocyte types was calculated based on Giemsa stained blood smear preparations. Data were analyzed descriptively. The results showed that hemoglobin levels and erythrocyte numbers tend to increase in line to the e-cigarette aerosol exposure as a compensation for hypoxic conditions. The leukocyte numbers also tends to increase, although the increase is not in line to the increase of exposure frequency and duration. The percentage of lymphocytes, monocytes and eosinophils tend to increase compared to the control group, while the percentage of neutrophils tends to decrease. Thus e-cigarette aerosol exposure affected the haematological profiles of rats
{"title":"Pengaruh Paparan Aerosol Rokok Elektrik Terhadap Profil Hematologis Rattus norvegicus","authors":"Natasha Fiorentina Kusumawati, Tetri Widiyani, Shanti Listyawati","doi":"10.14710/baf.7.2.2022.75-82","DOIUrl":"https://doi.org/10.14710/baf.7.2.2022.75-82","url":null,"abstract":" Rokok elektrik (RE) merupakan inovasi terbaru rokok yaitu tanpa proses pembakaran tar dan tembakau dan dianggap lebih aman dibandingkan rokok konvensional. Penelitian ini bertujuan mengetahui pengaruh paparan aerosol RE terhadap profil hematologis tikus putih (Rattus norvegicus) yang meliputi kadar hemoglobin, eritrosit, leukosit serta jenis leukosit. Dalam penelitian ini hewan uji Rattus norvegicus dibagi menjadi 4 kelompok dengan perlakuan yang berbeda yaitu diberi 0x paparan/hari (kontrol); 1x paparan/hari, 2x paparan/hari, dan 3x paparan/hari selama 14 hari. Setiap paparan dilakukan selama 5 menit (10 kali hembusan). Sampel darah diambil pada hari ke-0, hari ke-7 dan ke-14 melalui sinus orbitalis. Jumlah eritrosit dan leukosit dihitung dengan metode hitung bilik Neubauer. Kadar hemoglobin ditentukan menggunakan metode Sahli, dan persentase jenis leukosit dihitung dari preparat apus darah dengan pewarnaan Giemsa. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan terjadi peningkatan kadar hemoglobin dan jumlah eritrosit yang sejalan dengan peningkatan frekuensi dan durasi paparan RE sebagai kompensasi dari kondisi hipoksia. Jumlah leukosit juga cenderung meningkat, tetapi peningkatannya tidak linier dengan peningkatan frekuensi dan durasi paparan RE. Persentase limfosit, monosit dan eosinofil dibandingkan kontrol cenderung meningkat sedangkan persentase neutrofil cenderung menurun. Dengan demikian paparan RE dapat mempengaruhi profil hematologis hewan uji. E-cigarettes are the latest innovations of cigarettes without process of burning tar and tobacco. They are considered safer than conventional cigarettes. The purpose of this study was to determine the effect of e-cigarette aerosol exposure on the hematological profiles of Wistar rats (Rattus norvegicus). Rats were exposed to e-cigarette aerosol in different frequencies: 0x (control); once; twice; and 3x exposure/day. The experiment was ended on day 14th. Blood samples were taken through the orbital sinus on the day 0, 7th and 14th. Erythrocyte and leucocyte numbers were measured in Neubauer chamber count. Hemoglobin levels was determined by using Sahli method, and the percentage of leukocyte types was calculated based on Giemsa stained blood smear preparations. Data were analyzed descriptively. The results showed that hemoglobin levels and erythrocyte numbers tend to increase in line to the e-cigarette aerosol exposure as a compensation for hypoxic conditions. The leukocyte numbers also tends to increase, although the increase is not in line to the increase of exposure frequency and duration. The percentage of lymphocytes, monocytes and eosinophils tend to increase compared to the control group, while the percentage of neutrophils tends to decrease. Thus e-cigarette aerosol exposure affected the haematological profiles of rats ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123176460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-08DOI: 10.14710/baf.7.1.2022.66-72
Munifatul Izzati
Kacang hijau (Vigna radiata) merupakan salah satu sumber pangan yang kaya nilai nutrisi. Pertumbuhan penduduk yang tinggi di Indonesia memerlukan suplai tanaman pangan yang mencukupi. Beberapa spesies kacang kacangan dapat ditingkatkan petumbuhan dan produksinya dengan perlakuan intensitas cahaya. Penurunan intensitas cahaya hingga ke level tertentu dapat berpengaruh pada pertumbuhan tanaman. Penelitian ini bertujuan untuk menganalisis pengaruh naungan terhadap pertumbuhan tanaman kacang hijau. Penelitian dilakkukan di Laboratorium Biologi Struktur dan Fungsi Tumbuhan. Kacang hijau ditanam dalam pot berdiameter 30 cm, dan tinggi 40 cm. kelompok perlakuan adalah tanaman kacang hijau yang diberi naungan paranet dgn intensitas cahaya 45%, sedangkan kelompok kontrol adalah tanaman kacang hijau tanpa naungan, dengan intensitas cahaya 100%. Penelitian dirancang menggunakan RAL. Data yang diperloleh diolah dengan uji T. Hasil penelitian menunjukkan bahwa pemberian naungan 45% menghasilkan petumbuhan dan produksi tanaman kacang hijau yang lebih rendah Mung bean (Vigna radiata) is one source of food that is rich in nutritional value. High population growth in Indonesia requires an adequate supply of food crops. Several species of legumes can be increased in growth and production by light intensity treatment. A decrease in light intensity to a certain level can affect plant growth. This study aims to analyze the effect of shade on the growth of green bean plants. The research was carried out in the Biology Laboratory of Plant Structure and Function. Green beans are planted in pots with a diameter of 30 cm, and a height of 40 cm. the treatment group was mung bean plants that were shaded with paranet with a light intensity of 45%, while the control group was a mung bean plant without shade, with a light intensity of 100%. The study was designed using RAL. The data obtained were processed by T test. The results showed that the provision of 45% shade resulted in lower growth and production of mung bean plants
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