Pub Date : 2022-04-06DOI: 10.14710/baf.7.1.2022.51-59
Ida Ayu Putu Sugiantari, A. A. Sukmaningsih, I. M. S. Wijana
Danau Batur saat ini sudah mulai tercemar akibat tingginya beban pencemaran yang masuk ke perairan disebabkan oleh aktivitas masyarakat di sekitar danau. Penelitian ini bertujuan untuk melihat kerusaka struktur histologi hati, insang, dan lambung, ikan nila serta mengetahui jenis logam berat yang terdapat di perairan Danau Batur. Penelitian ini menggunakan 5 titik lokasi pengambilan sampel ikan nila. Sampel dibuat sayatan histologi organ dengan metode embedding dan analisis logam berat dengan Atomic Absorption Spectrophotometer. Hasil skoring pada insang luar, diperoleh kejadian patologi berturut-turut hiperplasia, edema lamela sekunder, dan fusi lamela paling berat 25% pada 4 lokasi, 18,7% pada lokasi 5, dan 25,00% pada lokasi 2. Pada insang dalam menunjukkan berturut-turut hiperplasia, edema lamela sekunder dan fusi lamela paling berat dengan frekuensi masing-masing 25,00% pada 4 lokasi, 25,00% pada lokasi 5, dan 25,00% pada lokasi 4. Lambung ditemukan kerusakan berupasel kariolisis, infiltrasi sel radang, dan desquamasi epitel. Histologi hati menunjukkan adanya kariolisis, karioreksis, dan degenerasi melemak. Konsentrasi logam berat di organ hati terdeteksi Fe dengan konsentrasi paling besar 3,96 ppm pada lokasi 2 dan Cu dengan konsentrasi paling besar 0,7 ppm pada lokasi 5. Ditemukannya kerusakan organ serta adanya logam berat, menandakan bahwa perairan Danau Batur mengalami pencemaran oleh aktivitas manusia di sekitar danau. Lake Batur is currently starting to be polluted due to the high pollution load enters into waters which caused of the people activities around the lake. This study aims to observe the histological structure damage of the liver, gills, and stomach, on the tilapia fishes, as well as to determine heavy metal types found in the Batur Lake water. This study was carried out 5 points of Batur Lake. The fish samples were prepared histological section by using the embedding method. Heavy metals were determined by using the Atomic Absorption Spectrophotometer. The scoring on the external gills showed there were pathological occurrences of hyperplasia, secondary lamellae edema, and lamellae fusion with most severe frequency of 25% at 4 locations, 18.7% at location 5, and 20% at location 2 respectively. Internal gills showed hyperplasia, secondary lamellae edema and most severe lamella fusion with most severe frequency of 25% at 4 sites, 25% at location 5, 25% at location 4 respectively. The stomach damaged were karyolytic cells, inflammatory cell infiltration, and epithelial desquamation. Liver histology showed karyolysis, karyorrhexis, and fatty degeneration. The heavy metals Fe and Cu were detected in the liver with Fe with the highest concentration of 3.96 ppm (Fe) at location 2 and 0.7 ppm (Cu) at location 5. The discovery of organ damages and the presence of heavy metals on the tilapia fish body indicated that Batur Lake water has been polluted by human activity around the lake.
{"title":"Kajian Struktur Histologi Hati, Insang dan Lambung Ikan Nila (Oreochromis niloticus) di Danau Batur, Bangli","authors":"Ida Ayu Putu Sugiantari, A. A. Sukmaningsih, I. M. S. Wijana","doi":"10.14710/baf.7.1.2022.51-59","DOIUrl":"https://doi.org/10.14710/baf.7.1.2022.51-59","url":null,"abstract":"Danau Batur saat ini sudah mulai tercemar akibat tingginya beban pencemaran yang masuk ke perairan disebabkan oleh aktivitas masyarakat di sekitar danau. Penelitian ini bertujuan untuk melihat kerusaka struktur histologi hati, insang, dan lambung, ikan nila serta mengetahui jenis logam berat yang terdapat di perairan Danau Batur. Penelitian ini menggunakan 5 titik lokasi pengambilan sampel ikan nila. Sampel dibuat sayatan histologi organ dengan metode embedding dan analisis logam berat dengan Atomic Absorption Spectrophotometer. Hasil skoring pada insang luar, diperoleh kejadian patologi berturut-turut hiperplasia, edema lamela sekunder, dan fusi lamela paling berat 25% pada 4 lokasi, 18,7% pada lokasi 5, dan 25,00% pada lokasi 2. Pada insang dalam menunjukkan berturut-turut hiperplasia, edema lamela sekunder dan fusi lamela paling berat dengan frekuensi masing-masing 25,00% pada 4 lokasi, 25,00% pada lokasi 5, dan 25,00% pada lokasi 4. Lambung ditemukan kerusakan berupasel kariolisis, infiltrasi sel radang, dan desquamasi epitel. Histologi hati menunjukkan adanya kariolisis, karioreksis, dan degenerasi melemak. Konsentrasi logam berat di organ hati terdeteksi Fe dengan konsentrasi paling besar 3,96 ppm pada lokasi 2 dan Cu dengan konsentrasi paling besar 0,7 ppm pada lokasi 5. Ditemukannya kerusakan organ serta adanya logam berat, menandakan bahwa perairan Danau Batur mengalami pencemaran oleh aktivitas manusia di sekitar danau. Lake Batur is currently starting to be polluted due to the high pollution load enters into waters which caused of the people activities around the lake. This study aims to observe the histological structure damage of the liver, gills, and stomach, on the tilapia fishes, as well as to determine heavy metal types found in the Batur Lake water. This study was carried out 5 points of Batur Lake. The fish samples were prepared histological section by using the embedding method. Heavy metals were determined by using the Atomic Absorption Spectrophotometer. The scoring on the external gills showed there were pathological occurrences of hyperplasia, secondary lamellae edema, and lamellae fusion with most severe frequency of 25% at 4 locations, 18.7% at location 5, and 20% at location 2 respectively. Internal gills showed hyperplasia, secondary lamellae edema and most severe lamella fusion with most severe frequency of 25% at 4 sites, 25% at location 5, 25% at location 4 respectively. The stomach damaged were karyolytic cells, inflammatory cell infiltration, and epithelial desquamation. Liver histology showed karyolysis, karyorrhexis, and fatty degeneration. The heavy metals Fe and Cu were detected in the liver with Fe with the highest concentration of 3.96 ppm (Fe) at location 2 and 0.7 ppm (Cu) at location 5. The discovery of organ damages and the presence of heavy metals on the tilapia fish body indicated that Batur Lake water has been polluted by human activity around the lake. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"598 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125594592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-06DOI: 10.14710/baf.7.1.2022.60-65
Indah Tri Intan Setiyaningrom, Sri Damanti, Endang Saptiningsih
Sorgum merupakan bahan pangan alternatif yang berpotensi besar dikembangkan di Indonesia. Monosodium glutamat merupakan bahan penguat rasa dengan komposisi berupa natrium dan glutamat, yang bermanfaat pada proses pertumbuhan tanaman. Penelitian ini bertujuan untuk mengkaji dosis efektif monosodium glutamat terhadap pola pertumbuhan tanaman sorgum. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal berupa dosis MSG dengan 4 taraf perlakuan, yaitu 0 g/tanaman, 3 g/tanaman, 6 g/tanaman dan 9 g/tanaman. Perlakuan diberikan dengan cara menaburkan MSG di sekitar daerah perakaran sorgum setiap satu minggu sekali. Parameter yang diukur adalah jumlah daun, luas daun dan tinggi tanaman yang didata setiap minggu sekali, dari minggu kedua sampai minggu kedelapan. Hasil penelitian menunjukkan bahwa tanaman sorgum dengan perlakuan MSG berpengaruh meningkatkan pertumbuhan dan mengubah pola pertumbuhan sorgum. Dosis 3 g/tanaman menunjukkan peningkatan pola pertumbuhan lebih cepat. Sorghum is an alternative crop with great potential to develop in Indonesia. Monosodium glutamate is a flavor enhancer with a composition of sodium and glutamate, which is beneficial for plant growth processes. This research aims to examine the effective dose of monosodium glutamate on the growth pattern of sorghum plants. This research used a single factor Completely Randomized Design (CRD) in the form of MSG dose with 4 treatment levels, namely 0 g/plant, 3 g/plant, 6 g/plant, and 9 g/plant. The treatment was given by sprinkling MSG around the sorghum root area once a week. The parameters measured were the number of leaves, leaf area, and plant height, which were recorded once a week, from the second week to the eighth week. The results showed that sorghum with MSG treatment affected increasing growth and changed the growth pattern of sorghum. The dose of 3 g/plant showed an increased growth pattern.
{"title":"Pola Pertumbuhan Tanaman Sorgum (Sorghum bicolor (L.) Moench) Dengan Perlakuan Monosodium Glutamat","authors":"Indah Tri Intan Setiyaningrom, Sri Damanti, Endang Saptiningsih","doi":"10.14710/baf.7.1.2022.60-65","DOIUrl":"https://doi.org/10.14710/baf.7.1.2022.60-65","url":null,"abstract":"Sorgum merupakan bahan pangan alternatif yang berpotensi besar dikembangkan di Indonesia. Monosodium glutamat merupakan bahan penguat rasa dengan komposisi berupa natrium dan glutamat, yang bermanfaat pada proses pertumbuhan tanaman. Penelitian ini bertujuan untuk mengkaji dosis efektif monosodium glutamat terhadap pola pertumbuhan tanaman sorgum. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal berupa dosis MSG dengan 4 taraf perlakuan, yaitu 0 g/tanaman, 3 g/tanaman, 6 g/tanaman dan 9 g/tanaman. Perlakuan diberikan dengan cara menaburkan MSG di sekitar daerah perakaran sorgum setiap satu minggu sekali. Parameter yang diukur adalah jumlah daun, luas daun dan tinggi tanaman yang didata setiap minggu sekali, dari minggu kedua sampai minggu kedelapan. Hasil penelitian menunjukkan bahwa tanaman sorgum dengan perlakuan MSG berpengaruh meningkatkan pertumbuhan dan mengubah pola pertumbuhan sorgum. Dosis 3 g/tanaman menunjukkan peningkatan pola pertumbuhan lebih cepat. Sorghum is an alternative crop with great potential to develop in Indonesia. Monosodium glutamate is a flavor enhancer with a composition of sodium and glutamate, which is beneficial for plant growth processes. This research aims to examine the effective dose of monosodium glutamate on the growth pattern of sorghum plants. This research used a single factor Completely Randomized Design (CRD) in the form of MSG dose with 4 treatment levels, namely 0 g/plant, 3 g/plant, 6 g/plant, and 9 g/plant. The treatment was given by sprinkling MSG around the sorghum root area once a week. The parameters measured were the number of leaves, leaf area, and plant height, which were recorded once a week, from the second week to the eighth week. The results showed that sorghum with MSG treatment affected increasing growth and changed the growth pattern of sorghum. The dose of 3 g/plant showed an increased growth pattern. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129357962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-05DOI: 10.14710/baf.7.1.2022.35-41
Dian Rosiana Fatikasari, E. Hastuti, Sri Haryanti
Pertumbuhan dan kandungan antosianin bayam merah sangat dipengaruhi oleh faktor pemupukan. Limbah teh merupakan pupuk organik yang memiliki unsur hara seperti nitrogen dan fosfor dimana penting dalam mempengaruhi pertumbuhan dan antosianin bayam merah. Penelitian bertujuan untuk mengetahui pengaruh limbah teh pada lama pengomposan yang berbeda terhadap pertumbuhan dan kandungan antosianin bayam merah. Penelitian dilakukan menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 5 ulangan. Perlakuan yang dimaksud adalah lama pengomposan 0, 1 dan 2 minggu. Perlakuan diaplikasikan dengan cara memberikan pupuk kompos limbah teh, tanah dan sekam dengan perbandingan 1:1:1 pada tanaman bayam merah yang seragam. Parameter yang diamati adalah tinggi tanaman, jumlah daun, berat basah tanaman, berat kering tanaman, panjang akar, berat basah akar, berat kering akar, berat kering tajuk, rasio tajuk akar dan nilai antosianin. Data yang dihasilkan dianalisis menggunakan Analysis of Variance (ANOVA) dilanjutkan uji lanjut Duncan Multiple Range Test (DMRT) pada taraf signifikansi 95%. Hasil penelitian menunjukkan perbedaan lama pengomposan berpengaruh meningkatkan tinggi tanaman, berat basah tanaman, berat kering tanaman, berat basah akar, nilai antosianin dan ratio tajuk akar. Perlakuan limbah teh lama pengomposan satu minggu memberikan rerata tertinggi terhadap pertumbuhan dan kandungan antosianin tanaman bayam merah. Growth of red amaranth and anthosyanin influenced by the fertilizer. Tea waste contain of nutrients such as nitrogen and phospor that important for red amaranth. The composting will effect for the fertilizer contain. Anthocyanin is a red- purple pigment. The aim of this experiment was to know the effects of tea waste in different composting time on red amaranth’s growth and anthocyanin. The design that used is Complate Random Design with 3 treatment and 5 replicates. The treatment of compost time were: 0 week, a week and 2 week. The parameters observed were plant height, leaf number, wet weight and dry weight of plant, root length, wet weight and dry weight of root, crown root ratio and anthocyanin. The result of data analysis using ANOVA followed by Duncan’s Multiple Range Test. The result showed that the difference of compost duration affected and improved the growth of plant height, leaf number, wet weight and dry weight of plant, root length, wet and dry weight of root, anthocyanin, crown root ratio. The treatment of tea waste by a week compost duration create the highest average for growth and anthocyanin of red amaranth.
红菠菜的生长和含量受到受精因子的高度影响。茶叶废物是一种有机肥料，含有氮和磷等营养成分，对影响红色菠菜的生长和花青素至关重要。这项研究的目的是确定茶的废物对红菠菜的生长和含量的长期营养素的影响。研究是使用一个包含3种治疗和5次重复的随机设计的研究。治疗规定的时间为0、1和2周。采用的方法是将茶、泥土和谷壳的废物肥料与均匀的菠菜植物相比:1。观察到的参数包括植物的高度、叶子的数量、植物的湿重、植物的干重、根的长度、湿根的重量、根的干重、头的干重、花青素的根与值之比。结果数据是用方差分析(ANOVA)继续测试邓肯多场试验(DMRT)进行分析的，其重要性为95%。研究表明，长期的育种差异会影响植物的高度、湿重、植物的干重、根系的湿重、花青素的价值和根茎拉奥。长期茶叶排泄物的治疗为红色菠菜植物的生长和含量提供了最高的营养。红色弹药和由fertilizer传播的病原体的增长。茶浪费了这样的营养成分:氮和磷对红色呼吁至关重要。电能会影响肥化的影响。青蒿素是一种红色的紫色色素。这个实验的目标是知道茶在红色弹药的不同时代浪费的影响。被使用的设计被压缩到三种试验和五种复制。报税时间是:0周，a周和2周。parameters观察到plant height, leaf number, wet weight and dry weight of plant, root length, wet weight and dry weight of root retio and anthocyanin。邓肯多次试验所遵循的ANOVA数据分析的结果。最近的说法表明，不同的是，植物的生长受影响和影响，叶子的生长，植物的根根和干重量，根根和干重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量，根根重量。本周的祈祷产生了最古老的红色呼吁的平均结果。
{"title":"Pertumbuhan dan Kandungan Antosianin Tanaman Bayam Merah (Alternanthera amoena Voss) Setelah Perlakuan Limbah Teh Pada Lama Pengomposan Yang Berbeda","authors":"Dian Rosiana Fatikasari, E. Hastuti, Sri Haryanti","doi":"10.14710/baf.7.1.2022.35-41","DOIUrl":"https://doi.org/10.14710/baf.7.1.2022.35-41","url":null,"abstract":"Pertumbuhan dan kandungan antosianin bayam merah sangat dipengaruhi oleh faktor pemupukan. Limbah teh merupakan pupuk organik yang memiliki unsur hara seperti nitrogen dan fosfor dimana penting dalam mempengaruhi pertumbuhan dan antosianin bayam merah. Penelitian bertujuan untuk mengetahui pengaruh limbah teh pada lama pengomposan yang berbeda terhadap pertumbuhan dan kandungan antosianin bayam merah. Penelitian dilakukan menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 5 ulangan. Perlakuan yang dimaksud adalah lama pengomposan 0, 1 dan 2 minggu. Perlakuan diaplikasikan dengan cara memberikan pupuk kompos limbah teh, tanah dan sekam dengan perbandingan 1:1:1 pada tanaman bayam merah yang seragam. Parameter yang diamati adalah tinggi tanaman, jumlah daun, berat basah tanaman, berat kering tanaman, panjang akar, berat basah akar, berat kering akar, berat kering tajuk, rasio tajuk akar dan nilai antosianin. Data yang dihasilkan dianalisis menggunakan Analysis of Variance (ANOVA) dilanjutkan uji lanjut Duncan Multiple Range Test (DMRT) pada taraf signifikansi 95%. Hasil penelitian menunjukkan perbedaan lama pengomposan berpengaruh meningkatkan tinggi tanaman, berat basah tanaman, berat kering tanaman, berat basah akar, nilai antosianin dan ratio tajuk akar. Perlakuan limbah teh lama pengomposan satu minggu memberikan rerata tertinggi terhadap pertumbuhan dan kandungan antosianin tanaman bayam merah. Growth of red amaranth and anthosyanin influenced by the fertilizer. Tea waste contain of nutrients such as nitrogen and phospor that important for red amaranth. The composting will effect for the fertilizer contain. Anthocyanin is a red- purple pigment. The aim of this experiment was to know the effects of tea waste in different composting time on red amaranth’s growth and anthocyanin. The design that used is Complate Random Design with 3 treatment and 5 replicates. The treatment of compost time were: 0 week, a week and 2 week. The parameters observed were plant height, leaf number, wet weight and dry weight of plant, root length, wet weight and dry weight of root, crown root ratio and anthocyanin. The result of data analysis using ANOVA followed by Duncan’s Multiple Range Test. The result showed that the difference of compost duration affected and improved the growth of plant height, leaf number, wet weight and dry weight of plant, root length, wet and dry weight of root, anthocyanin, crown root ratio. The treatment of tea waste by a week compost duration create the highest average for growth and anthocyanin of red amaranth.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125361476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-05DOI: 10.14710/baf.7.1.2022.42-50
Nuryanti Nuryanti, T. Suprihatin, Tyas Rini Saraswati
Kunyit termasuk dalam suku zingiberaceae yang mempunyai banyak manfaat dalam kesehatan. Kunyit mengandung senyawa kurkumin yang berfungsi sebagai antioksidan dan anti-inflamasi. Tujuan penelitian ini adalah menganalisis pengaruh pemberian serbuk kunyit dan kurkumin terhadap diferensial leukosit tikus putih yang diberi pakan hiperlipid yang diamati melalui jumlah granulosit, jumlah limfosit, dan jumlah monosit. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan 25 ekor Rattus norvegicus jantan berumur 21 hari yang dibagi dalam 5 kelompok perlakuan dengan 5 kali ulangan. Kelompok perlakuan meliputi: kontrol (C0), tikus diberi pakan hiperlipid (C1), tikus diberi pakan hiperlipid dan kurkumin 1,35 mg/200g BB/hari (C2), tikus diberi pakan hiperlipid dan serbuk kunyit 200 mg/200g BB/hari (C3), tikus diberi pakan hiperlipid dan simvastatin 0,18 mg/200g BB/hari (C4). Perlakuan diberikan selama 56 hari. Pengambilan sampel darah secara intrakardial, kemudian dilanjutkan dengan pengukuran jumlah granulosit, jumlah limfosit, dan jumlah monosit menggunakan hematology analyzer. Data penelitian dianalisis dengan uji Anova. Hasil menunjukkan bahwa tidak terdapat perbedaan bermakna (p>0,05) pada jumlah monosit, namun terdapat perbedaan bermakna pada jumlah granulosit dan jumlah limfosit. Kesimpulan dari penelitian ini adalah pemberian kurkumin dan serbuk kunyit pada tikus yang diberi pakan hiperlipid tidak menyebabkan perubahan pada diferensial leukosit dan masih berada pada keadaan normal.Turmeric is included in the zingiberaceae family which has many benefits in health. Turmeric contains curcumin compounds as antioxidants and anti-inflamatory. The purpose of this study was to analyze the effect of giving turmeric powder and curcumin on differential leukocytes of white rats given hyperlipid feeds which were observed through the number of granulocytes, the number of lymphocytes, and the number of monocytes. This study was an experimental study with a completely randomized design (CRD), using 25 male Rattus norvegicus 21 days old, divided into 5 treatment groups with 5 replications. The treatment group included control (C0), rats fed hyperlipid (C1), rats given hyperlipid feed and curcumin 1,35 mg/200g wb/day (C2), rats given hyperlipid feed and turmeric powder 200 mg/200g wb/day (C3), rats given hyperlipid feed and simvastatin 0,18 mg/ 200g wb/day (C4). Intracardial blood sampling, then followed by measuring the number of segmented leukocyte, lymphocyte count, and monocyte count used hematology analyzer. The research data were analyzed using Anova test. The results showed that there was no significant difference (p> 0,05) in monocyte count, but there were significant differences in granulocyte count and lymphocyte count. The conclusion of this study is that presenting curcumin and turmeric powder to rats fed hyperlipid diet did not cause changes in the leukocyte differential and were still in normal conditions.
{"title":"Peran Serbuk Kunyit dan Kurkumin Terhadap Diferensial Leukosit Tikus Putih (Rattus norvegicus) yang Diberi Pakan Hiperlipid","authors":"Nuryanti Nuryanti, T. Suprihatin, Tyas Rini Saraswati","doi":"10.14710/baf.7.1.2022.42-50","DOIUrl":"https://doi.org/10.14710/baf.7.1.2022.42-50","url":null,"abstract":"Kunyit termasuk dalam suku zingiberaceae yang mempunyai banyak manfaat dalam kesehatan. Kunyit mengandung senyawa kurkumin yang berfungsi sebagai antioksidan dan anti-inflamasi. Tujuan penelitian ini adalah menganalisis pengaruh pemberian serbuk kunyit dan kurkumin terhadap diferensial leukosit tikus putih yang diberi pakan hiperlipid yang diamati melalui jumlah granulosit, jumlah limfosit, dan jumlah monosit. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan 25 ekor Rattus norvegicus jantan berumur 21 hari yang dibagi dalam 5 kelompok perlakuan dengan 5 kali ulangan. Kelompok perlakuan meliputi: kontrol (C0), tikus diberi pakan hiperlipid (C1), tikus diberi pakan hiperlipid dan kurkumin 1,35 mg/200g BB/hari (C2), tikus diberi pakan hiperlipid dan serbuk kunyit 200 mg/200g BB/hari (C3), tikus diberi pakan hiperlipid dan simvastatin 0,18 mg/200g BB/hari (C4). Perlakuan diberikan selama 56 hari. Pengambilan sampel darah secara intrakardial, kemudian dilanjutkan dengan pengukuran jumlah granulosit, jumlah limfosit, dan jumlah monosit menggunakan hematology analyzer. Data penelitian dianalisis dengan uji Anova. Hasil menunjukkan bahwa tidak terdapat perbedaan bermakna (p>0,05) pada jumlah monosit, namun terdapat perbedaan bermakna pada jumlah granulosit dan jumlah limfosit. Kesimpulan dari penelitian ini adalah pemberian kurkumin dan serbuk kunyit pada tikus yang diberi pakan hiperlipid tidak menyebabkan perubahan pada diferensial leukosit dan masih berada pada keadaan normal.Turmeric is included in the zingiberaceae family which has many benefits in health. Turmeric contains curcumin compounds as antioxidants and anti-inflamatory. The purpose of this study was to analyze the effect of giving turmeric powder and curcumin on differential leukocytes of white rats given hyperlipid feeds which were observed through the number of granulocytes, the number of lymphocytes, and the number of monocytes. This study was an experimental study with a completely randomized design (CRD), using 25 male Rattus norvegicus 21 days old, divided into 5 treatment groups with 5 replications. The treatment group included control (C0), rats fed hyperlipid (C1), rats given hyperlipid feed and curcumin 1,35 mg/200g wb/day (C2), rats given hyperlipid feed and turmeric powder 200 mg/200g wb/day (C3), rats given hyperlipid feed and simvastatin 0,18 mg/ 200g wb/day (C4). Intracardial blood sampling, then followed by measuring the number of segmented leukocyte, lymphocyte count, and monocyte count used hematology analyzer. The research data were analyzed using Anova test. The results showed that there was no significant difference (p> 0,05) in monocyte count, but there were significant differences in granulocyte count and lymphocyte count. The conclusion of this study is that presenting curcumin and turmeric powder to rats fed hyperlipid diet did not cause changes in the leukocyte differential and were still in normal conditions.","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131660177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-29DOI: 10.14710/baf.7.1.2022.27-34
N. Safitri, Della Widya Puspita, Junita Junita, Lily Nur Inda Sary, Retno Robiatul Al Adawiyah, E. Prihastanti, Sri Widodo Agung Suedy
Kerusakan cabai rawit (Capsicum frutescens L.) pascapanen dapat menurunkan kualitas sehingga dapat mempersingkat masa simpan cabai rawit. Cara untuk mempertahankan kualitas cabai rawit dapat menggunakan nanokitosan. Nanokitosan memiliki kemampuan membentuk lapisan tipis di permukaan produk segar dan antimikroba. Salah satu pembuatan nanokitosan adalah menggabungkan kitosan dan STPP dengan perbandingan tertentu. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh nanokitosan terhadap kadar air dan tekstur cabai rawit dan rasio kitosan dan STPP optimum dalam mempertahankan kualitas cabai rawit. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan tiga perlakuan yaitu P0 (kontrol), P1 (perlakuan nanokitosan 0,2% rasio 1:3) dan P2 (perlakuan nanokitosan 0,2% rasio 1:5). Cabai rawit digunakan yang dipanen usia 90 hari setelah tanam, memiliki warna warna oranye kemerahan dengan panjang kurang lebih 6 cm, lebar 0,90 cm dan bebas dari penyakit. Penelitian ini dilakukan selama 16 hari pada suhu 10oC dengan variabel penelitian kualitas cabai rawit kadar air, tekstur, dan letak kerusakan. Data dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa nanokitosan dapat mempertahankan kualitas cabai rawit yaitu kadar air, tekstur, dan dapa mengurangi kerusakan. Rasio kitosan dan STPP optimum untuk mempertahankan kualitas cabai rawit setelah panen adalah nanokitosan 0,2% (rasio kitosan:STPP 1:5). Damage of postharvest chili pepper (Capsicum frutescens L.) can reduce the quality and it can shorten the shelf life of chili pepper. To maintain the quality of chili pepper can by using nanochitosan. Nanochitosan has the ability to form a thin layer on the surface of fresh produce and is antimicrobial. One of the manufactures of nanochitosan is to combine chitosan and STPP with a certain ratio. The purpose of this study was to determine the effect of nanochitosan on the moisture content and texture of chili pepper and the optimum ratio of chitosan and STPP in maintaining the quality of chili pepper. This study used a completely randomized design (CRD) method with three treatments, P0 (control), P1 (0.2% nanochitosan treatment ratio 1:3), and P2 (0.2% nanochitosan treatment ratio 1:5). Chili pepper is harvested 90 days after planting, has a reddish-orange color with a length of approximately 6 cm, a width of 0.90 cm, and is free from disease. This research was conducted for 16 days at a temperature of 10oC, with research variables of chili pepper quality, moisture content, texture, and location of the damage. Data were analyzed using the ANOVA test and continued with the DMRT test. The results showed that nanochitosan was able to maintain the quality of chili pepper, namely water content, texture, and can reduce damage. The optimum ratio of chitosan and STPP to preserve the quality of chili pepper after harvest is 0.2% nanochitosan (ratio of chitosan: STPP 1:5).
{"title":"Penyimpanan Cabai Rawit (Capsicum frutescens L.) dengan Pelapisan Nanokitosan pada Suhu Rendah","authors":"N. Safitri, Della Widya Puspita, Junita Junita, Lily Nur Inda Sary, Retno Robiatul Al Adawiyah, E. Prihastanti, Sri Widodo Agung Suedy","doi":"10.14710/baf.7.1.2022.27-34","DOIUrl":"https://doi.org/10.14710/baf.7.1.2022.27-34","url":null,"abstract":"Kerusakan cabai rawit (Capsicum frutescens L.) pascapanen dapat menurunkan kualitas sehingga dapat mempersingkat masa simpan cabai rawit. Cara untuk mempertahankan kualitas cabai rawit dapat menggunakan nanokitosan. Nanokitosan memiliki kemampuan membentuk lapisan tipis di permukaan produk segar dan antimikroba. Salah satu pembuatan nanokitosan adalah menggabungkan kitosan dan STPP dengan perbandingan tertentu. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh nanokitosan terhadap kadar air dan tekstur cabai rawit dan rasio kitosan dan STPP optimum dalam mempertahankan kualitas cabai rawit. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan tiga perlakuan yaitu P0 (kontrol), P1 (perlakuan nanokitosan 0,2% rasio 1:3) dan P2 (perlakuan nanokitosan 0,2% rasio 1:5). Cabai rawit digunakan yang dipanen usia 90 hari setelah tanam, memiliki warna warna oranye kemerahan dengan panjang kurang lebih 6 cm, lebar 0,90 cm dan bebas dari penyakit. Penelitian ini dilakukan selama 16 hari pada suhu 10oC dengan variabel penelitian kualitas cabai rawit kadar air, tekstur, dan letak kerusakan. Data dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa nanokitosan dapat mempertahankan kualitas cabai rawit yaitu kadar air, tekstur, dan dapa mengurangi kerusakan. Rasio kitosan dan STPP optimum untuk mempertahankan kualitas cabai rawit setelah panen adalah nanokitosan 0,2% (rasio kitosan:STPP 1:5). Damage of postharvest chili pepper (Capsicum frutescens L.) can reduce the quality and it can shorten the shelf life of chili pepper. To maintain the quality of chili pepper can by using nanochitosan. Nanochitosan has the ability to form a thin layer on the surface of fresh produce and is antimicrobial. One of the manufactures of nanochitosan is to combine chitosan and STPP with a certain ratio. The purpose of this study was to determine the effect of nanochitosan on the moisture content and texture of chili pepper and the optimum ratio of chitosan and STPP in maintaining the quality of chili pepper. This study used a completely randomized design (CRD) method with three treatments, P0 (control), P1 (0.2% nanochitosan treatment ratio 1:3), and P2 (0.2% nanochitosan treatment ratio 1:5). Chili pepper is harvested 90 days after planting, has a reddish-orange color with a length of approximately 6 cm, a width of 0.90 cm, and is free from disease. This research was conducted for 16 days at a temperature of 10oC, with research variables of chili pepper quality, moisture content, texture, and location of the damage. Data were analyzed using the ANOVA test and continued with the DMRT test. The results showed that nanochitosan was able to maintain the quality of chili pepper, namely water content, texture, and can reduce damage. The optimum ratio of chitosan and STPP to preserve the quality of chili pepper after harvest is 0.2% nanochitosan (ratio of chitosan: STPP 1:5). ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128089589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Penelitian ini bertujuan untuk mengetahui efek perlindungan ekstrak serai wangi Cymbopogon nardus (C. nardus) terhadap induksi timbal asetat. Sebanyak 25 mencit jantan strain BALB/C secara acak dibagi menjadi lima kelompok. Kelompok kontrol negatif hanya mendapat injeksi sub cutan Na-CMC 0,5% selama 30 hari ; kontrol positif diinjeksi sub cutan timbal asetat sebanyak 7 mg/kg bb selama 5 hari; P1, P2, dan P3 secara berturut-turut diinjeksi sub cutan timbal asetat 7 mg/kg bb selama 5 hari kemudian dilanjutkan dengan injeksi ekstrak C. nardus sebanyak 25, 50, dan 100 mg/kg bb selama 25 hari. Pada akhir penelitian semua mencit dikorbankan dan dilakukan koleksi testis. Jaringan testis diproses menggunakan pewarnaan Hematoxylin-Eosin. Hasil menunjukkan bahwa terjadi peningkatan sel spermatogonia, sel spermatosit, dan sel spermatid serta diameter tubulus seminiferus dibandingkan dengan kelompok positif. Dosis optimal yang paling mampu memperbaiki kerusakan jaringan testis yaitu 25 mg/kg bb. This study aimed to determine the protective effect of Cymbopogon nardus (C. nardus) extract against lead acetate exposure. A total of 25 male mice BALB/C were randomly divided into five groups. The negative control group only received 0.5% Na-CMC subcutaneously for 30 days; positive control was given 7 mg/kg bw of lead acetate subcutaneously for 5 days; P1, P2, and P3 were sequentially injected with 7 mg/kg bw subcutaneously for 5 days followed by injection of 25, 50, and 100 mg/kg bw of C. nardus extract for 25 days. At the end of the study all mice were sacrificed and testes were collected. Testicular tissue using Hematoxylin-Eosin staining. The results showed that there was an increase in spermatogonia cells, spermatocytes, and spermatid cells as well as the diameter of the seminiferous tubules compared to the positive group. The optimal dose most capable of repairing testicular tissue damage was 25 mg/kg bw.
{"title":"Efek Proteksi Ekstrak Cymbopogon nardus pada Histologi Testis Mencit (Mus musculus) Setelah Induksi Timbal Asetat","authors":"Lilis Maghfuroh, Putri Ayu Ika Setyowati, Rofiatun Solekha, Khoirin Nisa, Nynda Ayu Nadira Savitri","doi":"10.14710/baf.7.1.2022.20-26","DOIUrl":"https://doi.org/10.14710/baf.7.1.2022.20-26","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui efek perlindungan ekstrak serai wangi Cymbopogon nardus (C. nardus) terhadap induksi timbal asetat. Sebanyak 25 mencit jantan strain BALB/C secara acak dibagi menjadi lima kelompok. Kelompok kontrol negatif hanya mendapat injeksi sub cutan Na-CMC 0,5% selama 30 hari ; kontrol positif diinjeksi sub cutan timbal asetat sebanyak 7 mg/kg bb selama 5 hari; P1, P2, dan P3 secara berturut-turut diinjeksi sub cutan timbal asetat 7 mg/kg bb selama 5 hari kemudian dilanjutkan dengan injeksi ekstrak C. nardus sebanyak 25, 50, dan 100 mg/kg bb selama 25 hari. Pada akhir penelitian semua mencit dikorbankan dan dilakukan koleksi testis. Jaringan testis diproses menggunakan pewarnaan Hematoxylin-Eosin. Hasil menunjukkan bahwa terjadi peningkatan sel spermatogonia, sel spermatosit, dan sel spermatid serta diameter tubulus seminiferus dibandingkan dengan kelompok positif. Dosis optimal yang paling mampu memperbaiki kerusakan jaringan testis yaitu 25 mg/kg bb. This study aimed to determine the protective effect of Cymbopogon nardus (C. nardus) extract against lead acetate exposure. A total of 25 male mice BALB/C were randomly divided into five groups. The negative control group only received 0.5% Na-CMC subcutaneously for 30 days; positive control was given 7 mg/kg bw of lead acetate subcutaneously for 5 days; P1, P2, and P3 were sequentially injected with 7 mg/kg bw subcutaneously for 5 days followed by injection of 25, 50, and 100 mg/kg bw of C. nardus extract for 25 days. At the end of the study all mice were sacrificed and testes were collected. Testicular tissue using Hematoxylin-Eosin staining. The results showed that there was an increase in spermatogonia cells, spermatocytes, and spermatid cells as well as the diameter of the seminiferous tubules compared to the positive group. The optimal dose most capable of repairing testicular tissue damage was 25 mg/kg bw. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117334611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-01DOI: 10.14710/baf.7.1.2022.1-10
Arifin Latifah, Kasiyati Kasiyati, Muhammad Anwar Djaelani
Hepar merupakan salah satu organ vital pada metabolisme aves. Penelitian ini bertujuan menganalisis penambahan tepung daun kelor dalam pakan yang dikombinasikan dengan paparan cahaya terhadap histomorfometri hepar itik pekin. Penelitian ini menggunakan Rancangan Acak Lengkap pola faktorial 4×2. Faktor utama berupa konsentrasi tepung daun kelor (0, 2, 4, dan 6%) dan warna lampu (putih dan hijau). Itik pekin yang digunakan berjumlah 48 ekor. Variabel pengukuran antara lain bobot hepar, bobot badan, indek hepatosomatik, dan diameter hepatosit. Data yang diperoleh dianalisis dengan uji Analysis of Variance (ANOVA) dua arah. Hasil menunjukkan bahwa paparan cahaya dengan kombinasi tepung daun kelor tidak berpengaruh signifikan (P>0,05) pada bobot hepar, bobot badan, dan indek hepatosomatik, namun berpengaruh signifikan (P<0,05) pada diameter hepatosit. Masing-masing faktor utama warna cahaya atau tepung daun kelor tidak berpengaruh signifikan pada semua variabel. Kesimpulan penelitian ini adalah penambahan tepung daun kelor dalam pakan yang dikombinasikan dengan paparan cahaya dapat meningkatkan ukuran diameter hepatosit dan memicu proliferasi sel–sel hepatosit. Proliferasi hepatosit mengarah pada regenerasi hepatosit terdapat pada kelompok 6% tepung daun kelor yang dikombinasikan dengan cahaya putih ataupun cahaya hijau. The liver is one of the vital organs in avian metabolism. This research aimed to analyzed the inclusion of Moringa leaf flour in feed with light exposure on the liver histomorphometry of Pekin ducks. This study used a completely Randomized Design in 2×4 arrangement, with main factors, the first factor was concentrations of Moringa flour and the second factor was color of light. Forty-eight Pekin ducks were used in this research. The variables measured in this research were liver weight, body weight, hepatosomatic index, and hepatocyte diameter. The data obtained were analyze using two-way Analysis of Variance. The light exposure with a combination of Moringa leaf flour had no significant effect on liver weight, body weight, and hepatosomatic index, but had a significant effect (P<0.05) on hepatocyte diameter. Meanwhile, the main factor, either light color or Moringa leaf flour had no significant effect on all variables. The conclusion of this study is Moringa leaf powder in the diet combined with light exposure could affect the histomorphometry of the liver which is characterized by an increase in hepatocyte cell diameter and the proliferation of hepatocyte cells. Liver cell proliferation leading to hepatocyte regeneration was found in the 6% group of Moringa leaf flour combined with white or green light.
Hepar是aves代谢的重要器官之一。这项研究的目的是分析饲料中牛油淀粉的增加，以及白垩质露。这项研究使用完整的随机模式设计阶乘4×2。酵母的浓度(0.2、4和6%)和灯的颜色(白色和绿色)是主要因素。使用的pekin鸭子有48只。测量变量包括重力、体重、内心身和病变直径。通过双向变量分析分析所得的数据。结果表明，暴露在牛黄粉与牛黄粉混合的光对hepar、体重和体质内的身体质量(P> 0.05)没有显著影响，但对hepatosit的直径有显著影响(P< 0.05)。光的主要颜色或颗粒的颜色对所有变量都没有显著影响。这项研究的结论是，在饲料中加入牛黄粉，再加上暴露在阳光下，会增加细胞核的直径，引发细胞的增殖。肝细胞再生导致肝细胞再生的是一组6%的酵母淀粉，与白光或绿光结合。肝脏是阿维安代谢的重要器官之一。这项研究将研究对象对象为北京鸭子的肝组织组织化。这个研究过去a完全Randomized设计在机构里,用2×4的玩factors,第一个因素是concentrations of Moringa面粉和光明之第二个因素是颜色。45只鸭子住在这个研究里在这个研究中，变量的测量是肝脏重量、身体重量、肝病指数和肝病直径。分析数据是用两种方法分析变量。轻暴露在死亡叶体重量、身体重量和肝病指数上没有明显的影响，但在hepatocyte直径有重要影响(P<0.05)。我的意思是，主要的因素，无论是颜色颜色的Moringa leaf flour，在所有变量上都没有有效的效果。这项研究的结论是，在与轻曝光的饮食中结合的是一种真菌波，它可能会影响肝病直径和肝病扩散的恶性肿瘤的组织变形。肝细胞再生率上升导致肝细胞呈白色或绿色。
{"title":"Histomorfometri Hepar Itik Peking (Anas platyrhynchos) setelah Penambahan Tepung Daun Kelor (Moringa oleifera Lam.) dalam Pakan yang Dikombinasikan dengan Paparan Cahaya","authors":"Arifin Latifah, Kasiyati Kasiyati, Muhammad Anwar Djaelani","doi":"10.14710/baf.7.1.2022.1-10","DOIUrl":"https://doi.org/10.14710/baf.7.1.2022.1-10","url":null,"abstract":"Hepar merupakan salah satu organ vital pada metabolisme aves. Penelitian ini bertujuan menganalisis penambahan tepung daun kelor dalam pakan yang dikombinasikan dengan paparan cahaya terhadap histomorfometri hepar itik pekin. Penelitian ini menggunakan Rancangan Acak Lengkap pola faktorial 4×2. Faktor utama berupa konsentrasi tepung daun kelor (0, 2, 4, dan 6%) dan warna lampu (putih dan hijau). Itik pekin yang digunakan berjumlah 48 ekor. Variabel pengukuran antara lain bobot hepar, bobot badan, indek hepatosomatik, dan diameter hepatosit. Data yang diperoleh dianalisis dengan uji Analysis of Variance (ANOVA) dua arah. Hasil menunjukkan bahwa paparan cahaya dengan kombinasi tepung daun kelor tidak berpengaruh signifikan (P>0,05) pada bobot hepar, bobot badan, dan indek hepatosomatik, namun berpengaruh signifikan (P<0,05) pada diameter hepatosit. Masing-masing faktor utama warna cahaya atau tepung daun kelor tidak berpengaruh signifikan pada semua variabel. Kesimpulan penelitian ini adalah penambahan tepung daun kelor dalam pakan yang dikombinasikan dengan paparan cahaya dapat meningkatkan ukuran diameter hepatosit dan memicu proliferasi sel–sel hepatosit. Proliferasi hepatosit mengarah pada regenerasi hepatosit terdapat pada kelompok 6% tepung daun kelor yang dikombinasikan dengan cahaya putih ataupun cahaya hijau. The liver is one of the vital organs in avian metabolism. This research aimed to analyzed the inclusion of Moringa leaf flour in feed with light exposure on the liver histomorphometry of Pekin ducks. This study used a completely Randomized Design in 2×4 arrangement, with main factors, the first factor was concentrations of Moringa flour and the second factor was color of light. Forty-eight Pekin ducks were used in this research. The variables measured in this research were liver weight, body weight, hepatosomatic index, and hepatocyte diameter. The data obtained were analyze using two-way Analysis of Variance. The light exposure with a combination of Moringa leaf flour had no significant effect on liver weight, body weight, and hepatosomatic index, but had a significant effect (P<0.05) on hepatocyte diameter. Meanwhile, the main factor, either light color or Moringa leaf flour had no significant effect on all variables. The conclusion of this study is Moringa leaf powder in the diet combined with light exposure could affect the histomorphometry of the liver which is characterized by an increase in hepatocyte cell diameter and the proliferation of hepatocyte cells. Liver cell proliferation leading to hepatocyte regeneration was found in the 6% group of Moringa leaf flour combined with white or green light. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"164 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127419150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-16DOI: 10.14710/baf.7.1.2022.11-19
Yasmin Aulia Rachma, Retno Indrati, S. Supriyadi
Lamtoro [Leucaena leucocephala (Lam.) de Wit] merupakan komoditas pangan lokal Indonesia yang berpotensi sebagai pangan sumber protein, namun biji lamtoro tua kurang diminati. Proses pengolahan yang dapat diaplikasikan pada biji lamtoro tua adalah perkecambahan, yang kemudian hasilnya biasa diolah menjadi berbagai makanan khas Indonesia. Lamtoro tua memiliki kulit biji yang tebal dan keras, sehingga perlu proses skarifikasi untuk memudahkan perkecambahan. Tujuan penelitian ini adalah untuk mengetahui karakteristik perkecambahan biji lamtoro pada perlakuan skarifikasi dengan variasi suhu dan durasi perendaman serta perubahan nilai gizi biji lamtoro setelah perkecambahan. Skarifikasi dilakukan dengan cara perendaman dalam air dengan suhu 50, 70, dan 90°C selama 5, 10, dan 15 menit kemudian dianalisis karakteristik perkecambahan berupa persen imbibisi, persen perkecambahan, dan kecepatan berkecambahnya. Kecambah dengan karakteristik perkecambahan terbaik dianalisis perubahan kandungan gizinya. Data diambil dengan pola Rancangan Acak Lengkap (RAL) pada tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa pola imbibisi yang terjadi pada biji lamtoro bersifat trifase. Perlakuan skarifikasi dengan air pada suhu 70°C selama 15 menit menghasilkan persen imbibisi, persen perkecambahan, dan kecepatan berkecambah tertinggi, sehingga uji perubahan kandungan gizi dilakukan pada perkecambahan dengan skarifikasi pada suhu 70°C selama 15 menit. Setelah perkecambahan selama 72 jam terjadi peningkatan kadar air dan kadar protein, serta penurunan kadar lemak, abu, dan karbohidrat. Lamtoro [Leucaena leucocephala (Lam.) de Wit] is a local Indonesian food commodity that can be a food source of protein, but old lamtoro seeds are less attractive. The processing process that can be applied to old lamtoro seeds is germination, which is then usually processed into various Indonesian specialities. Old lamtoro has a thick and hard seed coat, so it needs a scarification process to facilitate germination. The purpose of this study was to determine the germination characteristics of lamtoro seeds in scarification treatment with variations in temperature and soaking duration, as well as changes in the nutritional value of lamtoro seeds after germination. Scarification was carried out by immersion in water at a temperature of 50, 70, and 90°C for 5, 10, and 15 minutes and then analyzed for germination characteristics in the form of percent imbibition, germination percentage, and germination speed. Sprouts with the best germination characteristics were analyzed for changes in nutritional content. Data were taken using a completely randomized design (CRD) pattern at a 95% confidence level. The results showed that the imbibition pattern that occurred in lamtoro seeds was triphase. Scarification treatment with water at 70°C for 15 minutes resulted in the highest percent imbibition, germination percentage, and germination speed, so the test for changes in nutrient content was ca
Lamtoro (lamcaena leucocephala, Lam)是一种可能成为蛋白质来源的当地食品商品，但对老Lamtoro种子的需求较小。可以应用于古老的兰托罗种子的加工过程是发芽，然后将其加工成各种典型的印尼食品。老兰托罗的果皮又厚又硬，因此需要磨光来帮助发芽。本研究的目的是确定兰托罗种子发芽的特性，即温度和持续时间的变化以及兰托罗种子发芽后营养价值的改变。划痕方式完成浸泡水中温度50、70和90°C, 5, 10, 15分钟后特征分析百分之imbibisi百分之、发芽,发芽berkecambahnya速度。具有最佳发芽特性的新芽被分析其营养成分的变化。数据以95%的信任度进行完全随机设计。研究结果表明，兰托罗种子的石化模式是三相的。用水划痕待遇在70°C的温度下百分之15分钟赚百分之imbibisi,发芽,发芽的最高速度,因此营养含量变化进行试验和划痕在70°C的温度下发芽15分钟。发芽72小时后，水和蛋白质水平增加，脂肪、灰烬和碳水化合物水平下降。Lamtoro [Lam Leucaena leucocephala, de Wit]是一种当地的印尼食品来源，但古老的Lamtoro种子缺乏吸引力。这种加工过程可以应用于旧的lamtoro种子，然后通常应用于印尼的不同物种。老兰托罗有一件又厚又硬的衣服，所以它需要进行消毒处理。这项研究的目的是确定刻痕是carried out by immersion in water at a 50、70和90°C的温度为5、10和15分钟然后analyzed for germination characteristics百分之in the form of imbibition, germination percentage,和germination速度。对最佳的核特征进行分析，以消除大脑中核的变化。数据显示出95%的可靠性。据称，在南托罗种子中发生的感染模式是三相的。刻痕治疗with water at 70°C for百分之15分钟resulted in the最高imbibition, germination percentage,和germination速度,所以《nutrient为改变考试内容是carried out on germination by刻痕at a 70°C的温度为15分钟。经过72小时的消毒，水中的蛋白质和蛋白质的含量增加，在肥胖、灰和carbohydrate中发现了一个衍生物。
{"title":"Karakteristik Perkecambahan Biji Lamtoro [Leucaena leucocephala (Lam.)de Wit] pada Perlakuan Skarifikasi serta Perubahan Nilai Gizi Setelah Perkecambahan","authors":"Yasmin Aulia Rachma, Retno Indrati, S. Supriyadi","doi":"10.14710/baf.7.1.2022.11-19","DOIUrl":"https://doi.org/10.14710/baf.7.1.2022.11-19","url":null,"abstract":"Lamtoro [Leucaena leucocephala (Lam.) de Wit] merupakan komoditas pangan lokal Indonesia yang berpotensi sebagai pangan sumber protein, namun biji lamtoro tua kurang diminati. Proses pengolahan yang dapat diaplikasikan pada biji lamtoro tua adalah perkecambahan, yang kemudian hasilnya biasa diolah menjadi berbagai makanan khas Indonesia. Lamtoro tua memiliki kulit biji yang tebal dan keras, sehingga perlu proses skarifikasi untuk memudahkan perkecambahan. Tujuan penelitian ini adalah untuk mengetahui karakteristik perkecambahan biji lamtoro pada perlakuan skarifikasi dengan variasi suhu dan durasi perendaman serta perubahan nilai gizi biji lamtoro setelah perkecambahan. Skarifikasi dilakukan dengan cara perendaman dalam air dengan suhu 50, 70, dan 90°C selama 5, 10, dan 15 menit kemudian dianalisis karakteristik perkecambahan berupa persen imbibisi, persen perkecambahan, dan kecepatan berkecambahnya. Kecambah dengan karakteristik perkecambahan terbaik dianalisis perubahan kandungan gizinya. Data diambil dengan pola Rancangan Acak Lengkap (RAL) pada tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa pola imbibisi yang terjadi pada biji lamtoro bersifat trifase. Perlakuan skarifikasi dengan air pada suhu 70°C selama 15 menit menghasilkan persen imbibisi, persen perkecambahan, dan kecepatan berkecambah tertinggi, sehingga uji perubahan kandungan gizi dilakukan pada perkecambahan dengan skarifikasi pada suhu 70°C selama 15 menit. Setelah perkecambahan selama 72 jam terjadi peningkatan kadar air dan kadar protein, serta penurunan kadar lemak, abu, dan karbohidrat. Lamtoro [Leucaena leucocephala (Lam.) de Wit] is a local Indonesian food commodity that can be a food source of protein, but old lamtoro seeds are less attractive. The processing process that can be applied to old lamtoro seeds is germination, which is then usually processed into various Indonesian specialities. Old lamtoro has a thick and hard seed coat, so it needs a scarification process to facilitate germination. The purpose of this study was to determine the germination characteristics of lamtoro seeds in scarification treatment with variations in temperature and soaking duration, as well as changes in the nutritional value of lamtoro seeds after germination. Scarification was carried out by immersion in water at a temperature of 50, 70, and 90°C for 5, 10, and 15 minutes and then analyzed for germination characteristics in the form of percent imbibition, germination percentage, and germination speed. Sprouts with the best germination characteristics were analyzed for changes in nutritional content. Data were taken using a completely randomized design (CRD) pattern at a 95% confidence level. The results showed that the imbibition pattern that occurred in lamtoro seeds was triphase. Scarification treatment with water at 70°C for 15 minutes resulted in the highest percent imbibition, germination percentage, and germination speed, so the test for changes in nutrient content was ca","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126430878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-08DOI: 10.14710/baf.6.2.2021.138-145
Christina Horowidi, H. Sinay, R. Karuwal, Lona Parinussa
Perbedaan lokasi tumbuh dapat mengakibatkan perbedaan penampilan fenotipik tanaman yang dapat diamati secara morfologi dan anatomi seperti struktur anatomi sel sekretori. Tujuan penelitian ini adalah untuk mengetahui struktur sel sekretori daun jeruk kalamansi di pulau Ambon. Metode jelajah dilakukan pada 13 lokasi di Pulau Ambon untuk koleksi sampel, dan pada setiap lokasi diambil 3 tanaman sebagai 3 ulangan. Tiap tanaman diambil 5 daun pada setiap sisi pohon tanaman jeruk kalamansi dengan ukuran panjang 5-7 cm dan warna hijau tua. Pembuatan preparat mengikuti metode free hand section. Pengamatan menggunakan kamera Optilab pada mikroskop Olympus dengan perbesaran 400x. Pengukuran diameter sel menggunakan fitur measure pada software Image Ruster. Data kualitatif berupa struktur sel sekresi daun jeruk Kalamansi ditampilkan dalam bentuk gambar dan dideskripsikan sesuai hasil yang terlihat, sedangkan data hasil pengukuran diameter sel sekresi adalah rerata 3 ulangan dan ditampilkan sebagai mean ± standar deviasi (SD). Hasil penelitian menunjukkan adanya sel sekretori yang berjumlah satu sel. Struktur sel sekretori terdiri dari sel epitel, sel selubung, dan rongga sekretori. Bentuk sel sekresi ada yang bulat dan lonjong. Diameter rongga sekretori berkisar antara 106,08-167,60 µm. Berdasarkan hasil penelitian, maka dapat disimpulkan bahwa sel sekresi pada daun jeruk kalamansi pada lokasi-lokasi berbeda di Pulau Ambon bervariasi baik bentuk maupun ukurannya. Differences in habitat can induce differences in the phenotypic appearance of plants that can be observed morphologically and anatomically such as the anatomical structure of secretory cells. The purpose of this study was to determine the structure of the secretory cells in the leaves of Calamansy citrus in Ambon island. Tracking method was done for sample collections, and at each location 3 plants were taken as replicates. Each plant was taken 5 leaves with a length of 5-7 cm and dark green color. Prior to be observed, the fresh sample was done with free-hand section method. Microscopy observations were done by a light microscope at 400x magnification. Measurement of cell diameter was done by the measure feature in Image Ruster software. Qualitative data such as secretory cell structures of Calamansy citrus leaves were shown in form of images and described according to the results, while the data of the measurement of secretory cell diameters is the average of 3 replications and was shown as mean ± standard deviation (SD). The results showed the presence of secretory cells which amounted to one cell. The secretory cell structure is composed of epithelial cells, sheath cells, and secretory cavities. Cell shapes vary, including round and oval. The diameter of the secretory cell cavity ranges from 106.08-167.60 µm.
生长地点的差异可能会导致植物形态和解剖(如分泌物细胞的解剖结构)可见的表现性和解剖外观的差异。本研究的目的是研究安汶岛喀拉曼西柑橘叶的细胞结构。探索方法在安汶岛的13个地点进行样本收集，每一个地点采集3株植物作为3次重复。每一种植物都有5片叶子，长5英寸(5-7厘米)，颜色为深绿色。修复方法遵循自由手部分的方法。在奥林匹斯山的显微镜下使用光学摄像机进行的观测，其体积只有400倍。测量细胞直径使用了Ruster软件草图的测量功能。Kalamansi橙叶分泌细胞结构的定性数据显示图片和描述它们的结果看起来形式,而分泌细胞直径的测量结果是平均数据显示3《申命记》和作为平均值±标准差(SD)。研究表明，一个细胞的分泌物存在。分泌细胞的结构包括上皮细胞、鞘细胞和分泌物腔。存在的分泌物是圆形和椭圆形的。sekretori腔直径不等106,08-167,60µm。根据这项研究，可以得出结论，在安邦岛不同的地方，卡拉曼西橙子叶子上的分泌细胞形状和大小都不一样。栖息地的不同可以造成植物的不同，这种现象可以观察到植物的形态和解剖结构，就像秘密细胞的解剖结构一样。这项研究的目的是确定安邦柑橘树叶中的秘密细胞的结构。追踪方法是为样本收集完成的，每定位三种植物都采用复制品。每棵植物都以5根5厘米长的树枝和5绿色的颜色来呈现。在观察之前，新鲜的样本是用自由手的方法完成的。微观测结果是在400倍的放大镜下完成的。细胞直径的测量是由Ruster软件的测量功能完成的。qqe的美国数据如此secretory细胞structures Calamansy柑橘树叶是展示inform of images and described弥足results,当测量数据》》secretory diameters是不朽之平均3号房replications和美国是展示±均值standard deviation (SD)。据说，秘密细胞的存在存在于一个细胞中。秘书的结构暴露了上皮细胞、头骨和秘书的本质。细胞形状vary，包括圆形和椭圆形。《secretory细胞体直径从106 ranges 08-167。60µm。
{"title":"Struktur Sel Sekresi Daun Jeruk Kalamansi (Citrus microcarpa Bunge.) di Pulau Ambon","authors":"Christina Horowidi, H. Sinay, R. Karuwal, Lona Parinussa","doi":"10.14710/baf.6.2.2021.138-145","DOIUrl":"https://doi.org/10.14710/baf.6.2.2021.138-145","url":null,"abstract":" Perbedaan lokasi tumbuh dapat mengakibatkan perbedaan penampilan fenotipik tanaman yang dapat diamati secara morfologi dan anatomi seperti struktur anatomi sel sekretori. Tujuan penelitian ini adalah untuk mengetahui struktur sel sekretori daun jeruk kalamansi di pulau Ambon. Metode jelajah dilakukan pada 13 lokasi di Pulau Ambon untuk koleksi sampel, dan pada setiap lokasi diambil 3 tanaman sebagai 3 ulangan. Tiap tanaman diambil 5 daun pada setiap sisi pohon tanaman jeruk kalamansi dengan ukuran panjang 5-7 cm dan warna hijau tua. Pembuatan preparat mengikuti metode free hand section. Pengamatan menggunakan kamera Optilab pada mikroskop Olympus dengan perbesaran 400x. Pengukuran diameter sel menggunakan fitur measure pada software Image Ruster. Data kualitatif berupa struktur sel sekresi daun jeruk Kalamansi ditampilkan dalam bentuk gambar dan dideskripsikan sesuai hasil yang terlihat, sedangkan data hasil pengukuran diameter sel sekresi adalah rerata 3 ulangan dan ditampilkan sebagai mean ± standar deviasi (SD). Hasil penelitian menunjukkan adanya sel sekretori yang berjumlah satu sel. Struktur sel sekretori terdiri dari sel epitel, sel selubung, dan rongga sekretori. Bentuk sel sekresi ada yang bulat dan lonjong. Diameter rongga sekretori berkisar antara 106,08-167,60 µm. Berdasarkan hasil penelitian, maka dapat disimpulkan bahwa sel sekresi pada daun jeruk kalamansi pada lokasi-lokasi berbeda di Pulau Ambon bervariasi baik bentuk maupun ukurannya. Differences in habitat can induce differences in the phenotypic appearance of plants that can be observed morphologically and anatomically such as the anatomical structure of secretory cells. The purpose of this study was to determine the structure of the secretory cells in the leaves of Calamansy citrus in Ambon island. Tracking method was done for sample collections, and at each location 3 plants were taken as replicates. Each plant was taken 5 leaves with a length of 5-7 cm and dark green color. Prior to be observed, the fresh sample was done with free-hand section method. Microscopy observations were done by a light microscope at 400x magnification. Measurement of cell diameter was done by the measure feature in Image Ruster software. Qualitative data such as secretory cell structures of Calamansy citrus leaves were shown in form of images and described according to the results, while the data of the measurement of secretory cell diameters is the average of 3 replications and was shown as mean ± standard deviation (SD). The results showed the presence of secretory cells which amounted to one cell. The secretory cell structure is composed of epithelial cells, sheath cells, and secretory cavities. Cell shapes vary, including round and oval. The diameter of the secretory cell cavity ranges from 106.08-167.60 µm. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133798328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-31DOI: 10.14710/baf.6.2.2021.131-137
Devi Ermawati, E. Prihastanti, E. Hastuti
Simplisia merupakan bahan alami sebagai bahan pembuatan obat yang belum mengalami pengolahan. Pengirisan merupakan salah satu tahap penting dalam pembuatan bahan simplisia untuk mempermudah dalam proses pengepakan, penyimpanan dan penggilingan. Pengirisan dapat dilakukan secara melintang atau membujur. Tujuan penelitian ini adalah mengetahui pengaruh arah dan tebal irisan rimpang terhadap rendemen flavonoid, berat kering dan performa simplisia umbi garut (Maranta arundinaceaL.) setelah pengeringan. Penelitian ini dilaksanakan di Laboratorium BSF Tumbuhan UNDIP. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial 2X2 dengan perlakuan kombinasi ketebalan (3 mm dan 5 mm) dan arah irisan (membujur dan melintang). Parameter penelitian meliputi berat kering dan performa simplisia yang meliputi warna, kekerasan, dan aroma pada simplisia kering. Analisis data menggunakan Anova dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan arah irisan berpengaruh terhadap berat kering simplisia, sedangkan interaksi antara tebal dan arah irisan tidak berpengaruh terhadap berat kering simplisia umbi garut. Arah irisan berpengaruh terhadap berat kering dan performa simplisia umbi garut, tebal irisan berpengaruh terhadap rendemen flavonoid. Crude simplisia is natural material for making medicine, which has not undergo processing, packing, drying and saving. Slicing is an important step in making medicine because in this process the loss of some parts could ease the process of packing and milling. Slicing could be done diagonally or longitudinally. A Different way of slicing could affect this simplisia. This study aimed to examine the influence of slicing direction, the thickness of slices, and its combination on the dry weight and the performance of arrowroot. This research was conducted in the Laboratory of BSF Tumbuhan UNIDIP. The design used was Completely Randomized Design with combination treatment of thickness (3mm and 5mm) and slice direction (diagonally and longitudinally). The parameter of this research used dry weight by weighing after the drying process and the performance (by measuring color, hardness, and the aroma of dried simplisia). The data analysis used Analysis of Variance. The findings showed that slice direction influenced the weight loss on simplisia, but the interaction did not influence on the dry weight of arrowroot simplisia. Slice direction affects dry weight and performance of arrowroot simplicia, slice thickness affects flavonoid yield.
{"title":"Pengaruh Arah dan Tebal Irisan Rimpang Terhadap Rendemen Flavonoid, Berat Kering dan Performa Simplisia Umbi Garut (Maranta arundinacea L.) Setelah Pengeringan","authors":"Devi Ermawati, E. Prihastanti, E. Hastuti","doi":"10.14710/baf.6.2.2021.131-137","DOIUrl":"https://doi.org/10.14710/baf.6.2.2021.131-137","url":null,"abstract":"Simplisia merupakan bahan alami sebagai bahan pembuatan obat yang belum mengalami pengolahan. Pengirisan merupakan salah satu tahap penting dalam pembuatan bahan simplisia untuk mempermudah dalam proses pengepakan, penyimpanan dan penggilingan. Pengirisan dapat dilakukan secara melintang atau membujur. Tujuan penelitian ini adalah mengetahui pengaruh arah dan tebal irisan rimpang terhadap rendemen flavonoid, berat kering dan performa simplisia umbi garut (Maranta arundinaceaL.) setelah pengeringan. Penelitian ini dilaksanakan di Laboratorium BSF Tumbuhan UNDIP. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial 2X2 dengan perlakuan kombinasi ketebalan (3 mm dan 5 mm) dan arah irisan (membujur dan melintang). Parameter penelitian meliputi berat kering dan performa simplisia yang meliputi warna, kekerasan, dan aroma pada simplisia kering. Analisis data menggunakan Anova dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan arah irisan berpengaruh terhadap berat kering simplisia, sedangkan interaksi antara tebal dan arah irisan tidak berpengaruh terhadap berat kering simplisia umbi garut. Arah irisan berpengaruh terhadap berat kering dan performa simplisia umbi garut, tebal irisan berpengaruh terhadap rendemen flavonoid. Crude simplisia is natural material for making medicine, which has not undergo processing, packing, drying and saving. Slicing is an important step in making medicine because in this process the loss of some parts could ease the process of packing and milling. Slicing could be done diagonally or longitudinally. A Different way of slicing could affect this simplisia. This study aimed to examine the influence of slicing direction, the thickness of slices, and its combination on the dry weight and the performance of arrowroot. This research was conducted in the Laboratory of BSF Tumbuhan UNIDIP. The design used was Completely Randomized Design with combination treatment of thickness (3mm and 5mm) and slice direction (diagonally and longitudinally). The parameter of this research used dry weight by weighing after the drying process and the performance (by measuring color, hardness, and the aroma of dried simplisia). The data analysis used Analysis of Variance. The findings showed that slice direction influenced the weight loss on simplisia, but the interaction did not influence on the dry weight of arrowroot simplisia. Slice direction affects dry weight and performance of arrowroot simplicia, slice thickness affects flavonoid yield. ","PeriodicalId":127406,"journal":{"name":"Buletin Anatomi dan Fisiologi","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123253541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：