O. Goudouri, E. Kontonasaki, A. Theocharidou, N. Kantiranis, X. Chatzistavrou, P. Koidis, K. Paraskevopoulos
Apart from a good bioactive behavior, composite materials for dental applications should attain high mechani- cal properties. Thus, the aim of this work was the fabrication and characterization of novel sol-gel derived dental ceram- ics/bioactive glass composites and the investigation of their mechanical properties such as flexural strength and Weibull modulus. Fourier Transform Infrared Spectroscopy (FTIR) and Surface Scanning X-ray Diffractometry (XRD) revealed the crystallization of leucite. Flexural strength values were in the range of 5-50MPa and it was followed a linear rela- tionship between the amount of dental porcelain in the com- posites and the strength of the materials. In conclusion, den- tal ceramics/bioactive glass composites exhibit mechanical integrity and can be potentially used in restorative dentistry.
{"title":"Dental Ceramics/Bioactive Glass Composites: Characterization and Mechanical Properties Investigation","authors":"O. Goudouri, E. Kontonasaki, A. Theocharidou, N. Kantiranis, X. Chatzistavrou, P. Koidis, K. Paraskevopoulos","doi":"10.4303/BDA/D110257","DOIUrl":"https://doi.org/10.4303/BDA/D110257","url":null,"abstract":"Apart from a good bioactive behavior, composite materials for dental applications should attain high mechani- cal properties. Thus, the aim of this work was the fabrication and characterization of novel sol-gel derived dental ceram- ics/bioactive glass composites and the investigation of their mechanical properties such as flexural strength and Weibull modulus. Fourier Transform Infrared Spectroscopy (FTIR) and Surface Scanning X-ray Diffractometry (XRD) revealed the crystallization of leucite. Flexural strength values were in the range of 5-50MPa and it was followed a linear rela- tionship between the amount of dental porcelain in the com- posites and the strength of the materials. In conclusion, den- tal ceramics/bioactive glass composites exhibit mechanical integrity and can be potentially used in restorative dentistry.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116032467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Tagaya, T. Ikoma, Taro Takemura, S. Migita, M. Okuda, T. Yoshioka, N. Hanagata, J. Tanaka
The initial adhesion, spreading and cytoskeleton changes of fibroblast NIH3T3 cells onto hydroxyapatite (HAp) and oxidized poly(styrene) (PSox) sensors pre- adsorbed fetal bovine serum were analyzed by using a quartz crystal microbalance with dissipation technique and an atomic force microscopy (AFM). The frequency shift (Δf) and the dissipation shift (ΔD) curves on HAp nanocrystals showed the decrease in Δf with increasing ΔD for 80min and the subsequent increase in Δf with decreasing ΔD, while those on PSox showed the decrease in Δf for 120min with increasing ΔD for 50min and then with subsequent decreasing ΔD. The different adhesion behavior dependent on the surfaces was attributed to the cell-surface interactions; the cells on HAp had rough fibrous pseudopods and those on PSox had dense particulate pseudopods. The different structures indicated that the cytoskeleton changes and the rearrangement of the extracellular matrix at the interface caused the different adhesion behavior.
采用石英晶体微天平耗散技术和原子力显微镜(AFM)分析了成纤维细胞NIH3T3细胞在羟基磷灰石(HAp)和氧化聚苯乙烯(PSox)传感器预吸附胎牛血清上的初始粘附、扩散和细胞骨架变化。频移(Δf)和耗散HAp纳米晶体转变(ΔD)曲线显示的减少与增加ΔΔf D 80分钟和随后的增加与减少ΔΔf D,而PSox显示减少Δf 120分钟增加ΔD 50分钟,然后后续减少ΔD。不同表面的不同粘附行为归因于细胞-表面的相互作用;HAp上的细胞为粗纤维状假足，PSox上的细胞为致密颗粒状假足。不同的结构表明细胞骨架的改变和细胞外基质在界面处的重排导致了不同的粘附行为。
{"title":"Initial Adhesion Behavior of Fibroblasts onto Hydroxyapatite Nanocrystals","authors":"M. Tagaya, T. Ikoma, Taro Takemura, S. Migita, M. Okuda, T. Yoshioka, N. Hanagata, J. Tanaka","doi":"10.4303/BDA/D110165","DOIUrl":"https://doi.org/10.4303/BDA/D110165","url":null,"abstract":"The initial adhesion, spreading and cytoskeleton changes of fibroblast NIH3T3 cells onto hydroxyapatite (HAp) and oxidized poly(styrene) (PSox) sensors pre- adsorbed fetal bovine serum were analyzed by using a quartz crystal microbalance with dissipation technique and an atomic force microscopy (AFM). The frequency shift (Δf) and the dissipation shift (ΔD) curves on HAp nanocrystals showed the decrease in Δf with increasing ΔD for 80min and the subsequent increase in Δf with decreasing ΔD, while those on PSox showed the decrease in Δf for 120min with increasing ΔD for 50min and then with subsequent decreasing ΔD. The different adhesion behavior dependent on the surfaces was attributed to the cell-surface interactions; the cells on HAp had rough fibrous pseudopods and those on PSox had dense particulate pseudopods. The different structures indicated that the cytoskeleton changes and the rearrangement of the extracellular matrix at the interface caused the different adhesion behavior.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127148545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Apatite-forming ability is a prerequisite to ensure the direct bonding of biomedical materials to living bone. Recently, Shozui et al. reported that UV- irradiation enhanced the in vitro apatite-forming ability of the TiO2 layer (rutile) prepared by heat-treatment of titanium substrates in air. This study examined TiO2 (anatase)-covered Ti samples, derived by chemical and subsequent thermal treatment (CHT), in terms of their in vitro apatite-forming ability after UV-irradiation under varied conditions. UV-irradiation of CHT in air reduced the amount of active sites for apatite nucleation, while the UV-irradiation in water increased them. These contrary responses indicate that environmental factors of the UV- irradiation are important for controlling the in vitro apatite- forming ability of the anatase layer.
{"title":"Contrary Effects of UV-Irradiation on In Vitro Apatite-Forming Ability of TiO2 Layer in Simulated Body Fluid","authors":"K. Uetsuki, Y. Shirosaki, S. Hayakawa, A. Osaka","doi":"10.4303/BDA/D110142","DOIUrl":"https://doi.org/10.4303/BDA/D110142","url":null,"abstract":"Apatite-forming ability is a prerequisite to ensure the direct bonding of biomedical materials to living bone. Recently, Shozui et al. reported that UV- irradiation enhanced the in vitro apatite-forming ability of the TiO2 layer (rutile) prepared by heat-treatment of titanium substrates in air. This study examined TiO2 (anatase)-covered Ti samples, derived by chemical and subsequent thermal treatment (CHT), in terms of their in vitro apatite-forming ability after UV-irradiation under varied conditions. UV-irradiation of CHT in air reduced the amount of active sites for apatite nucleation, while the UV-irradiation in water increased them. These contrary responses indicate that environmental factors of the UV- irradiation are important for controlling the in vitro apatite- forming ability of the anatase layer.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130809416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
X. Chatzistavrou, D. Esteve, E. Hatzistavrou, E. Kontonasaki, K. Paraskevopoulos, A. Boccaccini
The aim of this study was to investigate the sol-gel technique to fabricate glass-ceramic composite materials. The produced materials have potential application in dental restorations and they are expected to exhibit better control of composition, microstructure and properties due to the intrinsic processing benefits of the sol-gel preparation method. The characterization of the fabricated composites was performed by a range of techniques. The microstructure and bioactive behavior of the materials before and after application of heat treatment were studied. Moreover, the capability of making coatings on dental glass-ceramic substrates using the new glass-ceramics was demonstrated. The formation of well attached coatings exhibiting bioactive behavior was documented.
{"title":"Sol-Gel Fabrication of Glass-Ceramic Composite Materials for Dental Application","authors":"X. Chatzistavrou, D. Esteve, E. Hatzistavrou, E. Kontonasaki, K. Paraskevopoulos, A. Boccaccini","doi":"10.4303/BDA/D110156","DOIUrl":"https://doi.org/10.4303/BDA/D110156","url":null,"abstract":"The aim of this study was to investigate the sol-gel technique to fabricate glass-ceramic composite materials. The produced materials have potential application in dental restorations and they are expected to exhibit better control of composition, microstructure and properties due to the intrinsic processing benefits of the sol-gel preparation method. The characterization of the fabricated composites was performed by a range of techniques. The microstructure and bioactive behavior of the materials before and after application of heat treatment were studied. Moreover, the capability of making coatings on dental glass-ceramic substrates using the new glass-ceramics was demonstrated. The formation of well attached coatings exhibiting bioactive behavior was documented.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"660 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122961267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, a variety of fibrous bioactive bioceramic-polymer composite scaffolds were made through electrospinning and their usefulness for bone tissue engineering was investigated. In this study, nanospheres of carbonated hydroxyapatite (CHA), which is a proven osteoconductive and biodegradable bioceramic, were synthesized using a nanoemulsion process and relatively high amounts of CHA nanospheres were successfully incorporated into electrospun poly(hydroxybutyrate-co- hydroxyvalerate) (PHBV) fibers with the aid of an ultrasonic power source. The biological evaluation of electrospun fibrous PHBV scaffolds and CHA/PHBV nanocomposite scaffolds were conducted through in vitro cell culture using the human osteoblast cell-line SaOS-2. Although both types of scaffolds supported the proliferation and spreading of SaOS-2 cells, the CHA/PHBV scaffolds caused significantly higher expression of alkaline phosphatase (ALP) activity of SaOS-2 cells than the PHBV scaffolds after 14 days of cell culture, indicating the potential of fibrous CHA/PHBV nanocomposite scaffolds for bone tissue regeneration applications.
{"title":"In Vitro Biological Evaluation of Fibrous PHBV Polymer and CHA/PHBV Nanocomposite Scaffolds Developed for Tissue Engineering Applications","authors":"H. Tong, Min Wang, W. Lu","doi":"10.4303/BDA/D110168","DOIUrl":"https://doi.org/10.4303/BDA/D110168","url":null,"abstract":"In recent years, a variety of fibrous bioactive bioceramic-polymer composite scaffolds were made through electrospinning and their usefulness for bone tissue engineering was investigated. In this study, nanospheres of carbonated hydroxyapatite (CHA), which is a proven osteoconductive and biodegradable bioceramic, were synthesized using a nanoemulsion process and relatively high amounts of CHA nanospheres were successfully incorporated into electrospun poly(hydroxybutyrate-co- hydroxyvalerate) (PHBV) fibers with the aid of an ultrasonic power source. The biological evaluation of electrospun fibrous PHBV scaffolds and CHA/PHBV nanocomposite scaffolds were conducted through in vitro cell culture using the human osteoblast cell-line SaOS-2. Although both types of scaffolds supported the proliferation and spreading of SaOS-2 cells, the CHA/PHBV scaffolds caused significantly higher expression of alkaline phosphatase (ALP) activity of SaOS-2 cells than the PHBV scaffolds after 14 days of cell culture, indicating the potential of fibrous CHA/PHBV nanocomposite scaffolds for bone tissue regeneration applications.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"196 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115652811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomohiro Matsumoto, M. Tadokoro, Kouji Hattori, Hajime Ougushi, J. Satou
Synthetic polymer (RADA16:PuraMatrix®) is an artificial peptide and self assembling. RADA16 is able to provide three dimensional constructs which hold mesenchy- mal stem cell (MSCs). We combined the MSCs into the polymer and fabricated MSC/RADA composites. The com- posites showed osteogenic differentiation in the culture con- dition. Supplementation of hydroxyapatite ceramics (HA) in the composites (MSC/RADA/HA composites) improved the mechanical property and demonstrated the extracellular bone like matrix formation in the culture condition. These results indicate the important property of HA ceramics for the proliferation and differentiation of MSCs in the polymer. The MSC/RADA/HA composites could be available in the field of bone tissue engineering.
{"title":"Osteogenic Differentiation of Mesenchymal Stem Cells/Polymer Composites with HA In Vitro","authors":"Tomohiro Matsumoto, M. Tadokoro, Kouji Hattori, Hajime Ougushi, J. Satou","doi":"10.4303/BDA/D110172","DOIUrl":"https://doi.org/10.4303/BDA/D110172","url":null,"abstract":"Synthetic polymer (RADA16:PuraMatrix®) is an artificial peptide and self assembling. RADA16 is able to provide three dimensional constructs which hold mesenchy- mal stem cell (MSCs). We combined the MSCs into the polymer and fabricated MSC/RADA composites. The com- posites showed osteogenic differentiation in the culture con- dition. Supplementation of hydroxyapatite ceramics (HA) in the composites (MSC/RADA/HA composites) improved the mechanical property and demonstrated the extracellular bone like matrix formation in the culture condition. These results indicate the important property of HA ceramics for the proliferation and differentiation of MSCs in the polymer. The MSC/RADA/HA composites could be available in the field of bone tissue engineering.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127772474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Theodorou, O. Goudouri, E. Kontonasaki, X. Chatzistavrou, L. Papadopoulou, N. Kantiranis, K. Paraskevopoulos
Melt derived bioactive glasses and sol-gel derived glasses have been extensively investigated and are reported to be bioactive. Investigation of the bioactivity was conducted in different media, like conventional Simulated Body Fluid (c-SBF), Dulbecco's Modified Eagle's Medium (DMEM) and Tris buffer solution. The aim of this work is to investigate and evaluate thein vitro bioactivity assessment of two commonly used bioactive glasses- 45S5 and 58S-soaked in DMEM and c-SBF, in order to compare the biological response of both glasses in organic and inorganic environ- ments. For the samples immersed in c-SBF, the onset of apatite formation on the surface of 45S5 grains was slightly delayed in comparison to 58S. After 3 days in DMEM 58S powders revealed the formation of a crystalline HCAp phase on the surface of all grains in contrary to 45S5, where a sparsely developed amorphous apatite phase was developed.
熔融衍生的生物活性玻璃和溶胶-凝胶衍生的玻璃已被广泛研究并报道具有生物活性。在常规模拟体液(c-SBF)、Dulbecco's Modified Eagle's Medium (DMEM)和Tris缓冲液等不同培养基中进行生物活性研究。本研究的目的是研究和评价两种常用的生物活性玻璃- 45S5和58s -在DMEM和c-SBF中浸泡的体外生物活性评价，以比较两种玻璃在有机和无机环境中的生物反应。在c-SBF中，45S5晶粒表面磷灰石形成的开始时间略晚于58S。在DMEM 58S粉末中放置3天后，发现所有颗粒表面都形成了结晶的HCAp相，而45S5则相反，在45S5中，形成了稀疏发育的无定形磷灰石相。
{"title":"Comparative Bioactivity Study of 45S5 and 58S Bioglasses in Organic and Inorganic Environment","authors":"G. Theodorou, O. Goudouri, E. Kontonasaki, X. Chatzistavrou, L. Papadopoulou, N. Kantiranis, K. Paraskevopoulos","doi":"10.4303/BDA/D110154","DOIUrl":"https://doi.org/10.4303/BDA/D110154","url":null,"abstract":"Melt derived bioactive glasses and sol-gel derived glasses have been extensively investigated and are reported to be bioactive. Investigation of the bioactivity was conducted in different media, like conventional Simulated Body Fluid (c-SBF), Dulbecco's Modified Eagle's Medium (DMEM) and Tris buffer solution. The aim of this work is to investigate and evaluate thein vitro bioactivity assessment of two commonly used bioactive glasses- 45S5 and 58S-soaked in DMEM and c-SBF, in order to compare the biological response of both glasses in organic and inorganic environ- ments. For the samples immersed in c-SBF, the onset of apatite formation on the surface of 45S5 grains was slightly delayed in comparison to 58S. After 3 days in DMEM 58S powders revealed the formation of a crystalline HCAp phase on the surface of all grains in contrary to 45S5, where a sparsely developed amorphous apatite phase was developed.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123559983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Goudouri, E. Kontonasaki, A. Theocharidou, L. Papadopoulou, X. Chatzistavrou, P. Koidis, K. Paraskevopoulos
The aim of this work is the investigation of the in vitro bioactivity of sol-gel derived dental ceramics/bioactive glass (DC/BG) composites in periodically renewed SBF, as they are reported to promote the development of an apatite layer on their surfaces even under static conditions. Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) were used to characterize the reacted samples as powders and as thermally treated disk shaped specimens. DC/BG composites—both as powders and as thermally treated specimens—presented bioactivity in periodically renewed c-SBF. The onset of apatite formation on the surface of all powdered samples was observed even after three days of immersion, while the apatite formation on the surface of the heated composite specimens was slightly delayed. Moreover, it was observed an almost linear delay of apatite development to the dental ceramic concentration, attributed to the, accordingly, increased non-bioactive leucite content.
{"title":"In Vitro Bioactivity Studies of Sol-Gel Derived Dental Ceramics/Bioactive Glass Composites in Periodically Renewed Biomimetic Solution","authors":"O. Goudouri, E. Kontonasaki, A. Theocharidou, L. Papadopoulou, X. Chatzistavrou, P. Koidis, K. Paraskevopoulos","doi":"10.4303/BDA/D110250","DOIUrl":"https://doi.org/10.4303/BDA/D110250","url":null,"abstract":"The aim of this work is the investigation of the in vitro bioactivity of sol-gel derived dental ceramics/bioactive glass (DC/BG) composites in periodically renewed SBF, as they are reported to promote the development of an apatite layer on their surfaces even under static conditions. Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) were used to characterize the reacted samples as powders and as thermally treated disk shaped specimens. DC/BG composites—both as powders and as thermally treated specimens—presented bioactivity in periodically renewed c-SBF. The onset of apatite formation on the surface of all powdered samples was observed even after three days of immersion, while the apatite formation on the surface of the heated composite specimens was slightly delayed. Moreover, it was observed an almost linear delay of apatite development to the dental ceramic concentration, attributed to the, accordingly, increased non-bioactive leucite content.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127313247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. V. D. Beucken, L. T. Jonge, A. Plachokova, J. Jansen
In order to improve the efficacy of mem- branes used for guided tissue regeneration treatments, collagen membranes were surface-modified with the pro-mineralization enzyme alkaline phosphatase (ALP). ALP was surface-immobilized using electrostatic spray deposition (ESD) to preserves the biological activity of the enzyme. Initially, the optimal ALP-deposition time was assessed using ALP-activity assays and an immersion exper- iment with subsequent measurements on calcium deposi- tion. These initial experiments demonstrated that optimally efficient membranes were obtained with an ALP deposition time of 30 minutes. Subsequently, ALP-coated and non- coated controls were enrolled in a cell culture experiment with primary rat bone marrow derived osteoblast-like cells. ALP-coated membranes demonstrated to have no effect on cell proliferation and morphology, but significantly accel- erated the mineralization of the extracellular matrix. These results show the potential of ALP as a surface-immobilized active enzyme in biomaterial research and justify animal experimental work on this topic to obtain in vivo data.
{"title":"Enzymatically Enhanced Guided Tissue Regeneration","authors":"J. V. D. Beucken, L. T. Jonge, A. Plachokova, J. Jansen","doi":"10.4303/BDA/D110158","DOIUrl":"https://doi.org/10.4303/BDA/D110158","url":null,"abstract":"In order to improve the efficacy of mem- branes used for guided tissue regeneration treatments, collagen membranes were surface-modified with the pro-mineralization enzyme alkaline phosphatase (ALP). ALP was surface-immobilized using electrostatic spray deposition (ESD) to preserves the biological activity of the enzyme. Initially, the optimal ALP-deposition time was assessed using ALP-activity assays and an immersion exper- iment with subsequent measurements on calcium deposi- tion. These initial experiments demonstrated that optimally efficient membranes were obtained with an ALP deposition time of 30 minutes. Subsequently, ALP-coated and non- coated controls were enrolled in a cell culture experiment with primary rat bone marrow derived osteoblast-like cells. ALP-coated membranes demonstrated to have no effect on cell proliferation and morphology, but significantly accel- erated the mineralization of the extracellular matrix. These results show the potential of ALP as a surface-immobilized active enzyme in biomaterial research and justify animal experimental work on this topic to obtain in vivo data.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131083033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Schade, M. Sikirić, K. Liefeith, H. Füredi-Milhofer
In order to evaluate the biocompatibility and bioactivity of recently described nanocomposite coatings, consisting of polyelectrolyte multilayers interspersed with calcium apatite crystals, extensive cell culture tests have been applied to coated and uncoated chemically etched tita- nium plates. The coatings had top calcium phosphate layers (PLL/PGA)10CaP((PLL/PGA)5CaP)4 (A) or top polyelec- trolyte multilayers, (PLL/PGA)10CaP((PLL/PGA)5CaP)4 (PLL/PGA)5 ,( B), where PLL and PGA are poly-l-lysine and poly-l-glutamic acid respectively and CaP is calcium deficient apatite. Before testing coated samples were crosslinked and subsequently stored for extended periods of time (18 or 32 weeks) under dry, sterile conditions. Nonspecific activities, e.g. cell adhesion, proliferation, vitality, activity of mitochondrial dehydrogenases and cell morphology in contact with the material surfaces were tested using the cell-line MC3T3-E1, while for testing osteoblast specific activities, (collagen type I synthesis and alkaline phosphatase activity) SAOS-2 cells were used. The results show excellent biological properties of chemically etched titanium which were even surpassed by surfaces covered with coating B, while coating A (top crystal layer) adversely affected cell proliferation and performance. The effect is explained by morphological observations, showing inhibited spreading of the cells on the rough surfaces of coating A. The results also show that coatings, when shelved under dry and sterile conditions are stable for up to 8 months.
{"title":"Interaction of Cells with Organic-Inorganic Nanocomposite Coatings for Titanium Implants","authors":"R. Schade, M. Sikirić, K. Liefeith, H. Füredi-Milhofer","doi":"10.4303/BDA/D110159","DOIUrl":"https://doi.org/10.4303/BDA/D110159","url":null,"abstract":"In order to evaluate the biocompatibility and bioactivity of recently described nanocomposite coatings, consisting of polyelectrolyte multilayers interspersed with calcium apatite crystals, extensive cell culture tests have been applied to coated and uncoated chemically etched tita- nium plates. The coatings had top calcium phosphate layers (PLL/PGA)10CaP((PLL/PGA)5CaP)4 (A) or top polyelec- trolyte multilayers, (PLL/PGA)10CaP((PLL/PGA)5CaP)4 (PLL/PGA)5 ,( B), where PLL and PGA are poly-l-lysine and poly-l-glutamic acid respectively and CaP is calcium deficient apatite. Before testing coated samples were crosslinked and subsequently stored for extended periods of time (18 or 32 weeks) under dry, sterile conditions. Nonspecific activities, e.g. cell adhesion, proliferation, vitality, activity of mitochondrial dehydrogenases and cell morphology in contact with the material surfaces were tested using the cell-line MC3T3-E1, while for testing osteoblast specific activities, (collagen type I synthesis and alkaline phosphatase activity) SAOS-2 cells were used. The results show excellent biological properties of chemically etched titanium which were even surpassed by surfaces covered with coating B, while coating A (top crystal layer) adversely affected cell proliferation and performance. The effect is explained by morphological observations, showing inhibited spreading of the cells on the rough surfaces of coating A. The results also show that coatings, when shelved under dry and sterile conditions are stable for up to 8 months.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129177029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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