Pub Date : 2013-08-23DOI: 10.4172/2090-5025.S1-006
E. Yamamoto, N. Kato, Arata Isai, H. Nishikawa, M. Kusunoki, K. Yoshikawa, S. Hontsu
Restoration and protection of tooth enamel are of great importance in operative and conservative dentistry. Using a Pulsed Laser Deposition (PLD) method, we have successfully created a freestanding flexible double-layered sheet composed of a Hydroxyapatite (HAp) layer (4 m in thickness) coated with a Tricalcium Phosphate (TCP) layer (500 nm). In order to apply the newly developed HAp/TCP sheet to the restoration and protection of tooth enamel, the adhesive characteristics of the HAp/TCP sheet on the enamel were evaluated mechanically and microstructurally in the present study. The adhesive strength (5.7 MPa) between the HAp/TCP sheet and enamel was markedly higher than that (1.9 MPa) between the mono-layered HAp sheet and enamel. The electron microscopic observation revealed that HAp/TCP sheet was widely fused with the enamel. Therefore, the double-layered HAp/TCP sheet can be used as a material to promote the repair of tooth eruption and to maintain healthy dentine.
{"title":"Restoration of Tooth Enamel Using a Flexible Hydroxyapatite Sheet Coated with Tricalcium Phosphate","authors":"E. Yamamoto, N. Kato, Arata Isai, H. Nishikawa, M. Kusunoki, K. Yoshikawa, S. Hontsu","doi":"10.4172/2090-5025.S1-006","DOIUrl":"https://doi.org/10.4172/2090-5025.S1-006","url":null,"abstract":"Restoration and protection of tooth enamel are of great importance in operative and conservative dentistry. Using a Pulsed Laser Deposition (PLD) method, we have successfully created a freestanding flexible double-layered sheet composed of a Hydroxyapatite (HAp) layer (4 m in thickness) coated with a Tricalcium Phosphate (TCP) layer (500 nm). In order to apply the newly developed HAp/TCP sheet to the restoration and protection of tooth enamel, the adhesive characteristics of the HAp/TCP sheet on the enamel were evaluated mechanically and microstructurally in the present study. The adhesive strength (5.7 MPa) between the HAp/TCP sheet and enamel was markedly higher than that (1.9 MPa) between the mono-layered HAp sheet and enamel. The electron microscopic observation revealed that HAp/TCP sheet was widely fused with the enamel. Therefore, the double-layered HAp/TCP sheet can be used as a material to promote the repair of tooth eruption and to maintain healthy dentine.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127977894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-23DOI: 10.4172/2090-5025.S1-004
J. Lao, J. Lacroix, J. Nohra, N. Naaman, J. Sautier, douard Jallot
Studying the local release of strontium traces in vivo is of key interest, but calls for highly sensitive techniques besides providing an excellent (micronic) spatial resolution. In this context nuclear microprobes such as the PIXE (Particle-Induced X-ray Emission) technique, appear as powerful tools of investigation. Here, the in vivo behaviour of a Sr-delivering bioactive glass has been investigated through micro-PIXE analyses in connection with histological studies. New bone formation is observed after 6 weeks of implantation in rabbit femoral condyle. Interestingly, Sr traces are detected over a large area at the site of implantation, demonstrating the efficient release of Sr osteo inductive ions from the glass and their diffusion over several ten microns through the tissues. From its inorganic composition and content in traces of interest such as Zn, neo formed bone seems of higher quality for Sr-delivering particles compared to Sr-free particles, evidencing the positive effect of Sr in vivo.
{"title":"Bioavailability of Strontium Ions from Bioactive Glasses In Vivo: A Micro-PIXE Study of Trace Elements at the Bone Interface","authors":"J. Lao, J. Lacroix, J. Nohra, N. Naaman, J. Sautier, douard Jallot","doi":"10.4172/2090-5025.S1-004","DOIUrl":"https://doi.org/10.4172/2090-5025.S1-004","url":null,"abstract":"Studying the local release of strontium traces in vivo is of key interest, but calls for highly sensitive techniques besides providing an excellent (micronic) spatial resolution. In this context nuclear microprobes such as the PIXE (Particle-Induced X-ray Emission) technique, appear as powerful tools of investigation. Here, the in vivo behaviour of a Sr-delivering bioactive glass has been investigated through micro-PIXE analyses in connection with histological studies. New bone formation is observed after 6 weeks of implantation in rabbit femoral condyle. Interestingly, Sr traces are detected over a large area at the site of implantation, demonstrating the efficient release of Sr osteo inductive ions from the glass and their diffusion over several ten microns through the tissues. From its inorganic composition and content in traces of interest such as Zn, neo formed bone seems of higher quality for Sr-delivering particles compared to Sr-free particles, evidencing the positive effect of Sr in vivo.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116388563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-22DOI: 10.4172/2090-5025.S1-003
H. Nakada, Takehiro Watanabe, Takahiro Takahashi, K. Fujita, Y. Tanimoto, M. Teranishi, Takao Kato, Y. Kawai, T. Sakae, R. Legeros
This study aimed to determine changes in Bone Mineral Density (BMD) and bone strength of ovariectomized rats fed a synthetic diet of Mg, Zn, and F incorporated with carbonate apatite. Group 1 (n=4) was fed a Normal Mineral Diet (NMD) and Group 2 (n=4) was fed the Synthetic Diet (SD: 0.74% Ca, 0.48% P, 0.10% Mg, 365.4 ppm Zn, 51.01 ppm F and 0.27% CO3). Rats were sacrificed after eight weeks and the femur was isolated. The micro-CT results showed that values of BMD for Group 2 were higher than those of Group 1. Bone strength values of Group 2 measured by a 3-point bending test were significantly higher than those of Group 1. These results indicated that Group 2 stimulated bone quality, increased BMD and bone strength. The SD has the potential to prevent bone loss and as an osteoporosis therapy.
{"title":"A New Synthetic Diet Increased Bone Mineral Density and Strength of Ovariectomized Rat","authors":"H. Nakada, Takehiro Watanabe, Takahiro Takahashi, K. Fujita, Y. Tanimoto, M. Teranishi, Takao Kato, Y. Kawai, T. Sakae, R. Legeros","doi":"10.4172/2090-5025.S1-003","DOIUrl":"https://doi.org/10.4172/2090-5025.S1-003","url":null,"abstract":"This study aimed to determine changes in Bone Mineral Density (BMD) and bone strength of ovariectomized rats fed a synthetic diet of Mg, Zn, and F incorporated with carbonate apatite. Group 1 (n=4) was fed a Normal Mineral Diet (NMD) and Group 2 (n=4) was fed the Synthetic Diet (SD: 0.74% Ca, 0.48% P, 0.10% Mg, 365.4 ppm Zn, 51.01 ppm F and 0.27% CO3). Rats were sacrificed after eight weeks and the femur was isolated. The micro-CT results showed that values of BMD for Group 2 were higher than those of Group 1. Bone strength values of Group 2 measured by a 3-point bending test were significantly higher than those of Group 1. These results indicated that Group 2 stimulated bone quality, increased BMD and bone strength. The SD has the potential to prevent bone loss and as an osteoporosis therapy.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116375246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-22DOI: 10.4172/2090-5025.S1-001
Rc Richard, J. Dai, Sader, G. Soares, Rmsm Thir
Calcium Deficient Apatites (CDA’s) were prepared by hydrolysis method. The CDA’s were filtered, washed and calcined at 950â°C for 11h. X-ray diffraction demonstrated that β-TCP was obtained after calcining Mg-free CDA and β-TCMP or BCMP were obtained after calcining Mg-substituted CDAs, depending on the Mg/Ca molar ratio of the CDA. Physicochemical characterization was also performed by FT-IR spectroscopy and inductive coupled plasma. Phenolphthalein test was performed, in order to investigate the presence of calcium oxide according to the French standard NF S 94-066. SEM images assessed the morphology of the compounds. Cell viability assay (MTT), calcium nodule formation and the expression of alkaline phosphatase (ALP), osteocalcin, TGF-β1 and collagen were performed in MC3T3-E1 cell line. β-TCP, β-TCMP and BCMP obtained from hydrolysis method weren’t toxic and promoted cell proliferation, showing potential value in bone tissue engineering.
采用水解法制备了缺钙磷灰石。CDA经过过滤、洗涤，在950°C下煅烧11h。x射线衍射表明，煅烧无Mg CDA后得到β-TCP，煅烧取代Mg CDA后得到β-TCMP或BCMP，这取决于CDA的Mg/Ca摩尔比。利用红外光谱和电感耦合等离子体对其进行了理化表征。根据法国标准NF S 94-066进行酚酞测试，以调查氧化钙的存在。扫描电镜图像评估了化合物的形态。对MC3T3-E1细胞株进行细胞活力测定(MTT)、钙结节形成及碱性磷酸酶(ALP)、骨钙素、TGF-β1和胶原蛋白的表达。水解法得到的β-TCP、β-TCMP和BCMP均无毒且能促进细胞增殖，在骨组织工程中具有潜在的应用价值。
{"title":"Characterization of β-TCP, β-TCMP and BCMP Produced by Hydrolysis","authors":"Rc Richard, J. Dai, Sader, G. Soares, Rmsm Thir","doi":"10.4172/2090-5025.S1-001","DOIUrl":"https://doi.org/10.4172/2090-5025.S1-001","url":null,"abstract":"Calcium Deficient Apatites (CDA’s) were prepared by hydrolysis method. The CDA’s were filtered, washed and calcined at 950â°C for 11h. X-ray diffraction demonstrated that β-TCP was obtained after calcining Mg-free CDA and β-TCMP or BCMP were obtained after calcining Mg-substituted CDAs, depending on the Mg/Ca molar ratio of the CDA. Physicochemical characterization was also performed by FT-IR spectroscopy and inductive coupled plasma. Phenolphthalein test was performed, in order to investigate the presence of calcium oxide according to the French standard NF S 94-066. SEM images assessed the morphology of the compounds. Cell viability assay (MTT), calcium nodule formation and the expression of alkaline phosphatase (ALP), osteocalcin, TGF-β1 and collagen were performed in MC3T3-E1 cell line. β-TCP, β-TCMP and BCMP obtained from hydrolysis method weren’t toxic and promoted cell proliferation, showing potential value in bone tissue engineering.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115129606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-22DOI: 10.4172/2090-5025.S1-002
M. Lopez-Heredia, R. Gildenhaar, G. Berger, Ulf Linow, C. Gomes, J. Gnster, Houshm, M. Stiller, C. Knabe-Ducheyne
The effect of Fetal Bovine Serum (FBS) deprivation on survival and apoptosis of osteoblasts cultured on various calcium phosphates was studied. Test materials were two calcium alkali orthophosphates (materials denominated: GB9 and GB14), which were compared to β-tricalcium phosphate (TCP). Tissue culture polystyrene (PS) served as control. Test materials were characterized by X-Ray Diffraction and scanning electron microscopy. An apoptotic challenge assay entailing serum withdrawal was applied: MC3T3-E1 osteoblasts were cultured for 72h on the test materials in serum containing medium, followed by incubation in serum free medium for another 24h. Serum withdrawal is an apoptotic challenge, which creates a stressed environment. Cells cultured on the test specimens in serum containing medium served as control. The TUNEL Assay was employed to quantify the percentage of apoptotic cells. GB9 and GB14 displayed a significantly lower percentage of apoptotic cells than TCP. TCP had significantly fewer apoptotic cells than PS. The percentage of apoptotic cells on GB9 and GB14 was less than 10%, while the number of apoptotic cells found on the untreated control specimens ranged between 5 and 7%. These findings indicate that GB9 and GB14 endow osteoblasts cultured on them with a decreased sensitivity to apoptosis, which corresponds well with the results of previous in vitro and in vivo studies.
{"title":"Effect of Stressed and Unstressed Cell Culture Environments on the Viability of MC3T3 Cells with Calcium Phosphates","authors":"M. Lopez-Heredia, R. Gildenhaar, G. Berger, Ulf Linow, C. Gomes, J. Gnster, Houshm, M. Stiller, C. Knabe-Ducheyne","doi":"10.4172/2090-5025.S1-002","DOIUrl":"https://doi.org/10.4172/2090-5025.S1-002","url":null,"abstract":"The effect of Fetal Bovine Serum (FBS) deprivation on survival and apoptosis of osteoblasts cultured on various calcium phosphates was studied. Test materials were two calcium alkali orthophosphates (materials denominated: GB9 and GB14), which were compared to β-tricalcium phosphate (TCP). Tissue culture polystyrene (PS) served as control. Test materials were characterized by X-Ray Diffraction and scanning electron microscopy. An apoptotic challenge assay entailing serum withdrawal was applied: MC3T3-E1 osteoblasts were cultured for 72h on the test materials in serum containing medium, followed by incubation in serum free medium for another 24h. Serum withdrawal is an apoptotic challenge, which creates a stressed environment. Cells cultured on the test specimens in serum containing medium served as control. The TUNEL Assay was employed to quantify the percentage of apoptotic cells. GB9 and GB14 displayed a significantly lower percentage of apoptotic cells than TCP. TCP had significantly fewer apoptotic cells than PS. The percentage of apoptotic cells on GB9 and GB14 was less than 10%, while the number of apoptotic cells found on the untreated control specimens ranged between 5 and 7%. These findings indicate that GB9 and GB14 endow osteoblasts cultured on them with a decreased sensitivity to apoptosis, which corresponds well with the results of previous in vitro and in vivo studies.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125531872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Takashi Saito, M. Takemoto, S. Fujibayashi, M. Neo, T. Murakami, F. Miyaji, Toshikazu Nakamura
Control of the porous morphology and physic- ochemical characteristics of ceramics is important for osteoconduction. In this study, we determined the influence of the porous structure of glass-ceramics that contain apatite and wollastonite (GC-AW) on osteoconduction. For this purpose, we prepared GC-AW cylinders (6 × 15mm) with 5 different pore structures (macropore size, interconnecting pore size, and presence of micropores (pore size, approximately 1µm) for each material were as follows. Material 1000G: 800µm, 150-200µm, ±; material 1000S: 800µm, 175µm, �; material 300G: 250µm, 20µm, and ++; Material 300S: 250µm, 20µm, +; and 200S: 200µm, 50-80µm, �). To evaluate osteoconduction in vivo, these materials were implanted into holes (6mm diameter) made in rabbit femoral condyles. The percentage of bone ingrowth area significantly increased in the case of 300G, 300S, and 200S at 6 and 12 weeks. At 3 weeks after implantation, the rate of bone ingrowth was faster when 1000G, 1000S, and 200S were used than when 300G and 300S were used. These results suggest that GC-AW with a pore size of 800µm is unsuitable for bone growth, and rapid osteoconduction can be achieved at interconnecting pore sizes exceeding 50µm. Further, micropores may not be important for osteoconduction in the case of highly bioactive materials such as GC-AW.
{"title":"Quantitative Comparison of Osteoconduction between Porous Apatite and Wollastonite-Containing Glass-Ceramics with 5 Different Pore Sizes","authors":"Takashi Saito, M. Takemoto, S. Fujibayashi, M. Neo, T. Murakami, F. Miyaji, Toshikazu Nakamura","doi":"10.4303/BDA/D110152","DOIUrl":"https://doi.org/10.4303/BDA/D110152","url":null,"abstract":"Control of the porous morphology and physic- ochemical characteristics of ceramics is important for osteoconduction. In this study, we determined the influence of the porous structure of glass-ceramics that contain apatite and wollastonite (GC-AW) on osteoconduction. For this purpose, we prepared GC-AW cylinders (6 × 15mm) with 5 different pore structures (macropore size, interconnecting pore size, and presence of micropores (pore size, approximately 1µm) for each material were as follows. Material 1000G: 800µm, 150-200µm, ±; material 1000S: 800µm, 175µm, �; material 300G: 250µm, 20µm, and ++; Material 300S: 250µm, 20µm, +; and 200S: 200µm, 50-80µm, �). To evaluate osteoconduction in vivo, these materials were implanted into holes (6mm diameter) made in rabbit femoral condyles. The percentage of bone ingrowth area significantly increased in the case of 300G, 300S, and 200S at 6 and 12 weeks. At 3 weeks after implantation, the rate of bone ingrowth was faster when 1000G, 1000S, and 200S were used than when 300G and 300S were used. These results suggest that GC-AW with a pore size of 800µm is unsuitable for bone growth, and rapid osteoconduction can be achieved at interconnecting pore sizes exceeding 50µm. Further, micropores may not be important for osteoconduction in the case of highly bioactive materials such as GC-AW.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121239888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The weakness of bone plate made up from metal alloys and almost ceramics as well as thermosetting polymers is non biodegradable or very slow biodegradable. Thermoplastic and elastomeric polymers are degradable with various rates. A composite consisted of these polymers could be adjusted for required biodegradable rate. Beside, the composites would overcome mechanical behavior requirements to become promising device in tissue engineering. To support this point, fine hydroxyapatite (HAp) particles were penetrated in the composite. A hybrid composite with polymer mixture with HAp was fabricated by electrospinning method. Poly methyl methacrylate (PMMA) is a thermoplastic polymer, it was demon- strated good biocompatibility. However, PMMA inhibited inappropriate degradable rate in prosthetic treatment. Polyvinyl alcohol (PVA) is an elastomeric polymer; it has good biocompatibility, and rapidly bioabsorbable. These polymers were sprayed by electrospinning machine to form mats. HAp, was synthesized by microwave assisted process, and the contents in the composites were 0%, 2%, 4% and 6% by weight. Warm-pressing process was applied to form bone plate from mats. In MTT assay, the composites were non-toxic and showed good biocompatibility.
{"title":"Fabrication of Hybrid Composites Consists of Poly Methyl Methacrylate and Polyvinyl Alcohol and Hydroxyapatite","authors":"Viet Van Thai, Young‐Ki Min, Byong-Taek Lee","doi":"10.4303/BDA/D110171","DOIUrl":"https://doi.org/10.4303/BDA/D110171","url":null,"abstract":"The weakness of bone plate made up from metal alloys and almost ceramics as well as thermosetting polymers is non biodegradable or very slow biodegradable. Thermoplastic and elastomeric polymers are degradable with various rates. A composite consisted of these polymers could be adjusted for required biodegradable rate. Beside, the composites would overcome mechanical behavior requirements to become promising device in tissue engineering. To support this point, fine hydroxyapatite (HAp) particles were penetrated in the composite. A hybrid composite with polymer mixture with HAp was fabricated by electrospinning method. Poly methyl methacrylate (PMMA) is a thermoplastic polymer, it was demon- strated good biocompatibility. However, PMMA inhibited inappropriate degradable rate in prosthetic treatment. Polyvinyl alcohol (PVA) is an elastomeric polymer; it has good biocompatibility, and rapidly bioabsorbable. These polymers were sprayed by electrospinning machine to form mats. HAp, was synthesized by microwave assisted process, and the contents in the composites were 0%, 2%, 4% and 6% by weight. Warm-pressing process was applied to form bone plate from mats. In MTT assay, the composites were non-toxic and showed good biocompatibility.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130453164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Yeom, S. Chang, J. K. Park, J. Je, D. Yang, S. Choi, H. Shin, S. Hahn
A novel artificial bone substitute composed of bioactive MegaGen synthetic bone (MGSB) and hyaluronate (HA) hydrogels was successfully developed for bone tissue engineering applications. HA is known to play important roles in bone regeneration due to its angiogenic and osteoconductive characteristics. Accordingly, HA hydrogel was designed to supply HA continuously for effective bone regeneration by its controlled degrada- tion in vivo. Synchrotron X-ray bio-imaging clearly visualized 3-dimensional micron scale morphologies of effectively regenerated bones by the bone substitute of MGSB/Hyaluronate-Cystamine hydrogels implanted to the calvarial critical size bone defects in New Zealand white rabbits.
{"title":"Artificial Bone Substitute of MGSB and Hyaluronate Hydrogels","authors":"J. Yeom, S. Chang, J. K. Park, J. Je, D. Yang, S. Choi, H. Shin, S. Hahn","doi":"10.4303/BDA/D110162","DOIUrl":"https://doi.org/10.4303/BDA/D110162","url":null,"abstract":"A novel artificial bone substitute composed of bioactive MegaGen synthetic bone (MGSB) and hyaluronate (HA) hydrogels was successfully developed for bone tissue engineering applications. HA is known to play important roles in bone regeneration due to its angiogenic and osteoconductive characteristics. Accordingly, HA hydrogel was designed to supply HA continuously for effective bone regeneration by its controlled degrada- tion in vivo. Synchrotron X-ray bio-imaging clearly visualized 3-dimensional micron scale morphologies of effectively regenerated bones by the bone substitute of MGSB/Hyaluronate-Cystamine hydrogels implanted to the calvarial critical size bone defects in New Zealand white rabbits.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116879747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Puska, Henna Sihvonen, J. Korventausta, Gissur O⨠rlygsson, P. Vallittu, A. J. Aho
Considerable demand for the repair of bone defects cannot be met solely by using biological donor materials. Hence, the use of biocomposites will most likely be increased in reconstructive surgery in future. Metallic implants cannot be used in all cases, because the defects in the skeleton vary greatly, differing from each other in their shape, size, anatomic location, and physiological weight-bearing in the anatomic location. Therefore, more sophisticated orthopaedic materials (e.g. non-metallic fibre-reinforced composites and particulate filler composites) should be available for clinical practice. Our studies have focused on the development of injectable composites of biostable bone cements, i.e. in situ curable resin systems containing impregnated calcium ceramics particles. The properties of the bone cement composites aspire to simulate as closely as possible the mechanical and structural properties of bone. The purpose of this study was to evaluate the in vitro cell proliferation on the experimental injectable biostable polymer modified with calcium ceramics. In the course of proliferation, the cell activity on the calcium ceramics containing biostable polymer composite increased throughout the experiment. As a conclusion, this cell proliferation study indicated that the studied biocomposite has a good potential to promote cell interaction.
{"title":"Osteoblast and Fibroblast Culture Proliferation on Injectable Calcium Ceramics Polymer Composite","authors":"M. Puska, Henna Sihvonen, J. Korventausta, Gissur O⨠rlygsson, P. Vallittu, A. J. Aho","doi":"10.4303/BDA/D110161","DOIUrl":"https://doi.org/10.4303/BDA/D110161","url":null,"abstract":"Considerable demand for the repair of bone defects cannot be met solely by using biological donor materials. Hence, the use of biocomposites will most likely be increased in reconstructive surgery in future. Metallic implants cannot be used in all cases, because the defects in the skeleton vary greatly, differing from each other in their shape, size, anatomic location, and physiological weight-bearing in the anatomic location. Therefore, more sophisticated orthopaedic materials (e.g. non-metallic fibre-reinforced composites and particulate filler composites) should be available for clinical practice. Our studies have focused on the development of injectable composites of biostable bone cements, i.e. in situ curable resin systems containing impregnated calcium ceramics particles. The properties of the bone cement composites aspire to simulate as closely as possible the mechanical and structural properties of bone. The purpose of this study was to evaluate the in vitro cell proliferation on the experimental injectable biostable polymer modified with calcium ceramics. In the course of proliferation, the cell activity on the calcium ceramics containing biostable polymer composite increased throughout the experiment. As a conclusion, this cell proliferation study indicated that the studied biocomposite has a good potential to promote cell interaction.","PeriodicalId":127691,"journal":{"name":"Bioceramics Development and Applications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121403184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Oudadesse, M. Mami, R. Doebez-Sridi, P. Pellen, S. Jeanne, A. Zrineh, G. Cathelineau
Many types of bone substitutes exist to fill a bony defect. They must be compatible and bond with the host tissue without any formation of fibrous capsule. The direct apposition of bone to bioactive materials, including bioactive glasses and calcium phosphates, has already been demonstrated. In this work, different compns. of bioactive glasses elaborated by melting and rapid quenching were studied. They were based on SiO2-CaO-Na2O and P2O5. Several physicochem. methods like XRD, FTIR, SEM and ICP-OES were employed to characterize all bioactive glasses. They were investigated as bony biomaterials using "in vitro" assays. The aim was to evaluate their chem. reactivity and their bioactivity after soaking in simulated body fluid (SBF). [on SciFinder(R)]
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