Pub Date : 2024-07-30DOI: 10.30574/gscbps.2024.28.1.0245
Misbahul Huda, Sri Nuraini, Hartanti
Etawa Goat Milk can meet the body's vitamin and mineral needs. In Indonesia, Etawa goat milk is commonly sold and consumed fresh without adequate sterilization. As a result, this raw milk is at risk of contamination by various microorganisms. To address this issue and improve the microbial quality of Etawa goat milk, a study was carried out to evaluate the effect of incorporating an aqueous extract of dates and clove on the microbial quality of fresh Etawa goat milk. The Experimental design of this study is a completely randomized design consisting of 5 groups. Each group contains 25 mL fresh Etawa milk, and add with various volumes: 0.5 ml, 1.0 ml, 1.5 ml, 2.0 ml, and 2.5 ml of dates and cloves extract. The method used is the total plate count method. The identification of bacteria in untreated milk shows the presence of Escherichia coli, Flavobacterium sp, Kingella sp, Lactobacillus sp, Proteus sp, Pseudomonas sp, and Staphylococcus sp. The results indicate a reduction in the microbial count in milk treated with plant extract across all dosage ranges. The highest reduction was observed at the dosage of 2.5 ml, with a decrease of 44.3% (874,238 CFU/ml). The T-test yielded a P-value of 0.000, indicating a significant difference between the treated and untreated samples. This study found that date and clove extracts can improve the microbiological quality of fresh Etawa goat milk.
{"title":"Improving microbiological quality of Etawa goat milk with dates (Phoenix dactylifera) and cloves (Syzygium aromaticum) extract","authors":"Misbahul Huda, Sri Nuraini, Hartanti","doi":"10.30574/gscbps.2024.28.1.0245","DOIUrl":"https://doi.org/10.30574/gscbps.2024.28.1.0245","url":null,"abstract":"Etawa Goat Milk can meet the body's vitamin and mineral needs. In Indonesia, Etawa goat milk is commonly sold and consumed fresh without adequate sterilization. As a result, this raw milk is at risk of contamination by various microorganisms. To address this issue and improve the microbial quality of Etawa goat milk, a study was carried out to evaluate the effect of incorporating an aqueous extract of dates and clove on the microbial quality of fresh Etawa goat milk. The Experimental design of this study is a completely randomized design consisting of 5 groups. Each group contains 25 mL fresh Etawa milk, and add with various volumes: 0.5 ml, 1.0 ml, 1.5 ml, 2.0 ml, and 2.5 ml of dates and cloves extract. The method used is the total plate count method. The identification of bacteria in untreated milk shows the presence of Escherichia coli, Flavobacterium sp, Kingella sp, Lactobacillus sp, Proteus sp, Pseudomonas sp, and Staphylococcus sp. The results indicate a reduction in the microbial count in milk treated with plant extract across all dosage ranges. The highest reduction was observed at the dosage of 2.5 ml, with a decrease of 44.3% (874,238 CFU/ml). The T-test yielded a P-value of 0.000, indicating a significant difference between the treated and untreated samples. This study found that date and clove extracts can improve the microbiological quality of fresh Etawa goat milk.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"2 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141796267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.30574/gscbps.2024.28.1.0249
Hendianto, Felicia Aviana, Ida Sri Iswari, Ni Luh Indrayani, I Nyoman Wiryawan
Background: Methicillin-Sensible Staphylococcus aureus (MSSA) as the pathogen of the pericardial space is an uncommon case that can be fatal if untreated. The underlying disease accompany with infection that lead to cardiac tamponade can increase mortality rate of the patient. Case description: We present a 29 years old male patient with severe dispnea who was found to have cardiac tamponade secondary to a purulent pericardial effusion. He was also had end stage renal disease with regular hemodialysis. The diagnosis was suggested by clinical context, imaging, pericardial fluid analysis and was confirmed by culture. MSSA were isolated from 2 times pericardial fluid, peripheral blood and double lumen catheter exit site swab that were growth in blood agar only. Identification and susceptibility to antibiotics was assessed by Vitek2 Compact automated system (BioMerieux), represent sensitive to penicillin, cephalosporin, quinolone, aminoglycoside except macrolides. Administration of cefazolin intravenous for 5 days and also pericardial drainage result in a full recovery for the patient. Discussion: Purulent pericardial effusion is a rare condition that carries a high mortality rate as it can rapidly progress into cardiac tamponade. In developing countries, Mycobacterium tuberculosis is the most frequent cause of acute pericarditis followed by Haemophilus, Staphylococcus and Streptococcus. Prior to the advent of antibiotics, Staphylococcus aureus takes role as the predominant pathogen, this event was common as the result of hematogenous seeding such as catheter related hemodialysis in this patient. Conclusion: Prompt diagnosis of purulent pericardial effusion also initiation of appropriate antibiotic and pericardial drainage treatment are the mainstays of successful management of this rare but potentially lethal case.
{"title":"Methicillin-Sensible Staphylococcus aureus (MSSA) Pericardial Effusion Causing Cardiac Tamponade: A case report","authors":"Hendianto, Felicia Aviana, Ida Sri Iswari, Ni Luh Indrayani, I Nyoman Wiryawan","doi":"10.30574/gscbps.2024.28.1.0249","DOIUrl":"https://doi.org/10.30574/gscbps.2024.28.1.0249","url":null,"abstract":"Background: Methicillin-Sensible Staphylococcus aureus (MSSA) as the pathogen of the pericardial space is an uncommon case that can be fatal if untreated. The underlying disease accompany with infection that lead to cardiac tamponade can increase mortality rate of the patient. Case description: We present a 29 years old male patient with severe dispnea who was found to have cardiac tamponade secondary to a purulent pericardial effusion. He was also had end stage renal disease with regular hemodialysis. The diagnosis was suggested by clinical context, imaging, pericardial fluid analysis and was confirmed by culture. MSSA were isolated from 2 times pericardial fluid, peripheral blood and double lumen catheter exit site swab that were growth in blood agar only. Identification and susceptibility to antibiotics was assessed by Vitek2 Compact automated system (BioMerieux), represent sensitive to penicillin, cephalosporin, quinolone, aminoglycoside except macrolides. Administration of cefazolin intravenous for 5 days and also pericardial drainage result in a full recovery for the patient. Discussion: Purulent pericardial effusion is a rare condition that carries a high mortality rate as it can rapidly progress into cardiac tamponade. In developing countries, Mycobacterium tuberculosis is the most frequent cause of acute pericarditis followed by Haemophilus, Staphylococcus and Streptococcus. Prior to the advent of antibiotics, Staphylococcus aureus takes role as the predominant pathogen, this event was common as the result of hematogenous seeding such as catheter related hemodialysis in this patient. Conclusion: Prompt diagnosis of purulent pericardial effusion also initiation of appropriate antibiotic and pericardial drainage treatment are the mainstays of successful management of this rare but potentially lethal case.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"1 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141795749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.30574/gscbps.2024.28.1.0254
I Ketut Nata Ditama, Ni Luh Gde Sumardani, Anak Agung Putu Putra Wibawa, Ni Wayan Siti, I Gusti Nyoman Gde Bidura
This study aims to determine the effect of Moringa leaf flour supplementation in commercial feed on sexual behavior and semen quality of cross-bred Landrace boars. This research was carried out at the UPT Baturiti Regional Artificial Insemination Center (BIBD) located in Banjar Pekarangan, Baturiti Village, Baturiti District, Tabanan Regency, Bali Province, Indonesia. Observations used a completely randomized design consisting of two treatments, with 10 repetitions (number of semen collection). The treatments tried were boar fed without the addition of Moringa leaf meal (P0) and boar fed with the addition of 1% Moringa leaf meal (P1). The results showed that the addition of 1% Moringa leaf flour in feed (P1) showed significantly different results (P<0.05) on sexual behavior. The addition of Moringa leaf flour in commercial feed as much as 1% obtained significantly different results (P<0.05) in the semen evaluation value. It was concluded that the addition of 1% Moringa oleifera leaf meal in commercial feed had an effect on sexual behavior and could improve the semen quality of Landrace-crossbred boars.
{"title":"Supplementation of Moringa oleifera leaf meal in feed on sexual behavior and semen quality of Landrace-cross boars","authors":"I Ketut Nata Ditama, Ni Luh Gde Sumardani, Anak Agung Putu Putra Wibawa, Ni Wayan Siti, I Gusti Nyoman Gde Bidura","doi":"10.30574/gscbps.2024.28.1.0254","DOIUrl":"https://doi.org/10.30574/gscbps.2024.28.1.0254","url":null,"abstract":"This study aims to determine the effect of Moringa leaf flour supplementation in commercial feed on sexual behavior and semen quality of cross-bred Landrace boars. This research was carried out at the UPT Baturiti Regional Artificial Insemination Center (BIBD) located in Banjar Pekarangan, Baturiti Village, Baturiti District, Tabanan Regency, Bali Province, Indonesia. Observations used a completely randomized design consisting of two treatments, with 10 repetitions (number of semen collection). The treatments tried were boar fed without the addition of Moringa leaf meal (P0) and boar fed with the addition of 1% Moringa leaf meal (P1). The results showed that the addition of 1% Moringa leaf flour in feed (P1) showed significantly different results (P<0.05) on sexual behavior. The addition of Moringa leaf flour in commercial feed as much as 1% obtained significantly different results (P<0.05) in the semen evaluation value. It was concluded that the addition of 1% Moringa oleifera leaf meal in commercial feed had an effect on sexual behavior and could improve the semen quality of Landrace-crossbred boars.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"3 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141795940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.30574/gscbps.2024.28.1.0228
Akpan JL, Onwudiwe TC, Ikongshul Cecilia A, Igwe DO, Ofonakara U, Okam PC, Nwokike Matthew Onyemaechi, Akuodor GC
Aim: The leaves of S. biafrae have been used ethnomedically across Sub-Saharan Africa for the treatment of different diseases. The toxicological assessment and analgesic effects of the ethanol leaf extract of S. biafrae was the focus of the present study which was necessitated by the traditional uses of the extract in folk medicine. Methods: Acute and subacute toxicity testing were examined and parameters such as relative organ weight of both liver and kidney, changes in animal body weight, haematological indices as well as the examination of liver and kidney function parameters were all evaluated. For analgesic potential of the extract; acetic acid-induced writhing in mice and hot plate model were also evaluated. Results: The result of acute testing showed that the extract has an LD50 value of 3,492mg/kg in rats. The subacute toxicity tests showed no significant changes in the body weight of the animals throughout the duration of the experiment. However, there was significant reductions in the relative organ weight of both the liver and the kidney of the extract. The haematological parameters showed the extract had reduced PCV levels in rats as well as the serum WBC levels. The acetic acid-induced writhing test showed that all the concentrations of the extract used showed significant increase in their protective ability against acetic acid-induced writhing in mice. The hot plate model showed that the extract only produced significant inhibition against pain the first 30minutes of testing Conclusion: Findings show that, the ethanol leaf extract of S. biafrae though exhibited toxic potentials, it however demonstrated potent direct and central analgesic properties.
{"title":"Toxicological and analgesic evaluation of Solanecio biafrae ethanol leaf extract","authors":"Akpan JL, Onwudiwe TC, Ikongshul Cecilia A, Igwe DO, Ofonakara U, Okam PC, Nwokike Matthew Onyemaechi, Akuodor GC","doi":"10.30574/gscbps.2024.28.1.0228","DOIUrl":"https://doi.org/10.30574/gscbps.2024.28.1.0228","url":null,"abstract":"Aim: The leaves of S. biafrae have been used ethnomedically across Sub-Saharan Africa for the treatment of different diseases. The toxicological assessment and analgesic effects of the ethanol leaf extract of S. biafrae was the focus of the present study which was necessitated by the traditional uses of the extract in folk medicine. Methods: Acute and subacute toxicity testing were examined and parameters such as relative organ weight of both liver and kidney, changes in animal body weight, haematological indices as well as the examination of liver and kidney function parameters were all evaluated. For analgesic potential of the extract; acetic acid-induced writhing in mice and hot plate model were also evaluated. Results: The result of acute testing showed that the extract has an LD50 value of 3,492mg/kg in rats. The subacute toxicity tests showed no significant changes in the body weight of the animals throughout the duration of the experiment. However, there was significant reductions in the relative organ weight of both the liver and the kidney of the extract. The haematological parameters showed the extract had reduced PCV levels in rats as well as the serum WBC levels. The acetic acid-induced writhing test showed that all the concentrations of the extract used showed significant increase in their protective ability against acetic acid-induced writhing in mice. The hot plate model showed that the extract only produced significant inhibition against pain the first 30minutes of testing Conclusion: Findings show that, the ethanol leaf extract of S. biafrae though exhibited toxic potentials, it however demonstrated potent direct and central analgesic properties.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"10 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141795671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.30574/gscbps.2024.28.1.0270
Tam Minh Hoang, Anh Thị Ngọc Do, Thanh Thị Ngọc D ang
The study aims were to investigate the antibacterial ability of Avicennia alba Blume leaves collected in An Nghia, Dan Xay, Long Hoa belonging to Can Gio Mangrove Biosphere Reserve, Vietnam. Sample leaf powder of A. alba was used to make acetone extracts. Investigation on antibacterial ability of the extracts was performed by using agar well diffusion method. The results showed that acetone extracts from A. alba leaves exhibited antibacterial activity against Gram (+) bacteria: Bacillus cereus and Staphylococcus aureus but no inhibitory effects on Gram (-) bacteria: Pseudomonas aeruginosa and Escherichia coli. Antibacterial strength of extract increased with increasing extract concentration. Leaf extracts of A. alba growing An Nghia had higher inhibitory effect of B. cereus than two others, while leaf extracts of A. alba growing Dan Xay was more effectively inhibitory against S. aureus than the others. Only extract of A. alba growing An Nghia at concentrations of 900 mg mL-1 and 1000 mg mL-1 and A. alba growing Dan Xay at concentration of 1000 mg mL-1 had moderately antibacterial strength against B. cereus and S. aureus. The other tested extracts of A. alba had antibacterial activity at weak level.
本研究的目的是调查在越南 Can Gio 红树林生物圈保护区 An Nghia、Dan Xay、Long Hoa 采集的白花茜草（Avicennia alba Blume）叶片的抗菌能力。白花蛇舌草叶片粉末样品用于丙酮萃取。采用琼脂井扩散法对提取物的抗菌能力进行了研究。结果表明，白千层叶丙酮萃取物对革兰氏（+）细菌具有抗菌活性：蜡样芽孢杆菌和金黄色葡萄球菌具有抗菌活性，但对革兰氏（-）细菌没有抑制作用：铜绿假单胞菌和大肠杆菌没有抑制作用。萃取物的抗菌强度随萃取物浓度的增加而增加。生长在安义的白桦树叶萃取物对蜡样芽孢杆菌的抑制作用高于其他两种萃取物，而生长在丹霞的白桦树叶萃取物对金黄色葡萄球菌的抑制作用高于其他萃取物。只有浓度为 900 毫克/毫升-1 和 1000 毫克/毫升-1 的白桦属植物安雅和浓度为 1000 毫克/毫升-1 的白桦属植物丹赛的提取物对蜡样芽孢杆菌和金黄色葡萄球菌有中等程度的抗菌作用。白僵菌的其他测试提取物的抗菌活性较弱。
{"title":"Investigation on antibacterial ability of acetone extract from leaves of Avicennia alba Blume growing in the Can Gio Mangrove Biosphere Reserve, Vietnam","authors":"Tam Minh Hoang, Anh Thị Ngọc Do, Thanh Thị Ngọc D ang","doi":"10.30574/gscbps.2024.28.1.0270","DOIUrl":"https://doi.org/10.30574/gscbps.2024.28.1.0270","url":null,"abstract":"The study aims were to investigate the antibacterial ability of Avicennia alba Blume leaves collected in An Nghia, Dan Xay, Long Hoa belonging to Can Gio Mangrove Biosphere Reserve, Vietnam. Sample leaf powder of A. alba was used to make acetone extracts. Investigation on antibacterial ability of the extracts was performed by using agar well diffusion method. The results showed that acetone extracts from A. alba leaves exhibited antibacterial activity against Gram (+) bacteria: Bacillus cereus and Staphylococcus aureus but no inhibitory effects on Gram (-) bacteria: Pseudomonas aeruginosa and Escherichia coli. Antibacterial strength of extract increased with increasing extract concentration. Leaf extracts of A. alba growing An Nghia had higher inhibitory effect of B. cereus than two others, while leaf extracts of A. alba growing Dan Xay was more effectively inhibitory against S. aureus than the others. Only extract of A. alba growing An Nghia at concentrations of 900 mg mL-1 and 1000 mg mL-1 and A. alba growing Dan Xay at concentration of 1000 mg mL-1 had moderately antibacterial strength against B. cereus and S. aureus. The other tested extracts of A. alba had antibacterial activity at weak level.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"10 32","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141795847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.30574/gscbps.2024.28.1.0271
Houria MADANI, Amina Himri, Dounia El Moujtahide, El houcine Sebbar, Mohammed Choukri
Introduction: The aim of our study was to evaluate the analytical performance of the insulin dosage method, which was performed by the Alinity ci® automated system in the biochemistry laboratory of the Mohammed VI University Hospital in Oujda. Insulin acts as an essential role in regulating the body's energy supply, mainly by balancing blood sugar levels via the storage of glucose in the liver, muscles and adipose tissue. Materials and methods: We carried out a study of the performance of the Alinity ci® automated system, assessing repeatability and reproducibility in accordance with the COFRAC GTA 04 accreditation technical guide, which meets the quality requirements of standard ISO 15 189. Results: The results of our study show satisfactory repeatability for the three levels (Low; Medium; High) with CV1 = 2.44 %, CV2 = 1.76 %, CV3 = 1.33 %. The reproducibility of insulin was also satisfactory, with respect to the coefficient of variation (CV) for the three levels (Low, Medium, High), respectively CV1 = 3.16 %, CV2 = 5.78 %, CV3 = 2.88 %. Conclusion: The reliability of our laboratory's insulin assay results is demonstrated by the satisfactory results obtained in our study, which comply with RICOS and FSCB recommendations.
{"title":"Verification of the insulin dosage method using Abbott Alinity ci®: experience of the biochemistry laboratory, CHU Mohammed IV Oujda, Morocco","authors":"Houria MADANI, Amina Himri, Dounia El Moujtahide, El houcine Sebbar, Mohammed Choukri","doi":"10.30574/gscbps.2024.28.1.0271","DOIUrl":"https://doi.org/10.30574/gscbps.2024.28.1.0271","url":null,"abstract":"Introduction: The aim of our study was to evaluate the analytical performance of the insulin dosage method, which was performed by the Alinity ci® automated system in the biochemistry laboratory of the Mohammed VI University Hospital in Oujda. Insulin acts as an essential role in regulating the body's energy supply, mainly by balancing blood sugar levels via the storage of glucose in the liver, muscles and adipose tissue. Materials and methods: We carried out a study of the performance of the Alinity ci® automated system, assessing repeatability and reproducibility in accordance with the COFRAC GTA 04 accreditation technical guide, which meets the quality requirements of standard ISO 15 189. Results: The results of our study show satisfactory repeatability for the three levels (Low; Medium; High) with CV1 = 2.44 %, CV2 = 1.76 %, CV3 = 1.33 %. The reproducibility of insulin was also satisfactory, with respect to the coefficient of variation (CV) for the three levels (Low, Medium, High), respectively CV1 = 3.16 %, CV2 = 5.78 %, CV3 = 2.88 %. Conclusion: The reliability of our laboratory's insulin assay results is demonstrated by the satisfactory results obtained in our study, which comply with RICOS and FSCB recommendations.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"8 30","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141795816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.30574/gscbps.2024.28.1.0265
Maxime Benedict Paguirigan Tirol, Francis Augustine Autor Bajao, Pamela Guillena Biwang, Joenly Rose Avendaño Cardenas, Al-Farok Calandada Casidar, John Miguel Petallar Chiong, Sweet Elise Pelaez Labnao, Jovelyn Laurente Lacubtan, Hanagen Liclican Salvador, Madeleine Cabeltes Zamayla, Jevie Lyn Peralta-Tan Nery, Shosi Mari Salubre Larido, Sandra Enriquez Laguimun, Rowel Paningbatan Catchillar, Justin Dave Magracia Manantan, Jan Karlo Tiongson Ecalne
Cinnamomum mercadoi, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts were concentrated for 5 hours at 40°C to produce a green, syrupy substance. The extract was tested qualitatively, followed by FTIR analysis to confirm the results, before being subjected to the agar well diffusion method to evaluate its antibacterial properties against Staphylococcus aureus and Escherichia coli. The phytochemical evaluation and subsequent FTIR analysis revealed that the extract contained various phytochemicals, including glycosides, tannins, phytosterols, and terpenoids. The antibacterial testing revealed concentration-dependent inhibition that could be improved by purifying or isolating specific compounds from the extract. Additional research into other plant parts is suggested to enhance its biomedical and therapeutic applications.
{"title":"Evaluation of antibacterial property and phytochemical composition of the crude ethanolic extract from Kalingag (Cinnamomum mercadoi) barks","authors":"Maxime Benedict Paguirigan Tirol, Francis Augustine Autor Bajao, Pamela Guillena Biwang, Joenly Rose Avendaño Cardenas, Al-Farok Calandada Casidar, John Miguel Petallar Chiong, Sweet Elise Pelaez Labnao, Jovelyn Laurente Lacubtan, Hanagen Liclican Salvador, Madeleine Cabeltes Zamayla, Jevie Lyn Peralta-Tan Nery, Shosi Mari Salubre Larido, Sandra Enriquez Laguimun, Rowel Paningbatan Catchillar, Justin Dave Magracia Manantan, Jan Karlo Tiongson Ecalne","doi":"10.30574/gscbps.2024.28.1.0265","DOIUrl":"https://doi.org/10.30574/gscbps.2024.28.1.0265","url":null,"abstract":"Cinnamomum mercadoi, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts were concentrated for 5 hours at 40°C to produce a green, syrupy substance. The extract was tested qualitatively, followed by FTIR analysis to confirm the results, before being subjected to the agar well diffusion method to evaluate its antibacterial properties against Staphylococcus aureus and Escherichia coli. The phytochemical evaluation and subsequent FTIR analysis revealed that the extract contained various phytochemicals, including glycosides, tannins, phytosterols, and terpenoids. The antibacterial testing revealed concentration-dependent inhibition that could be improved by purifying or isolating specific compounds from the extract. Additional research into other plant parts is suggested to enhance its biomedical and therapeutic applications.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"2 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141796154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-30DOI: 10.30574/gscbps.2024.26.3.0077
Nahla Fathima, Nesniya T, Nivedhya Jith, Parvathy P S, Tamilselvan T, Rosemary Thomas
Chronic renal disease patients undergoing hemodialysis may face several problems that increase their chance of developing psychological suffering as hemodialysis is a time-consuming process. This study was conducted to assess the prevalence of psychiatric illness and to evaluate the impact of patient counselling on the quality of life. A total of 135 patients undergoing hemodialysis for more than 3 months were enrolled in the study. Patients who were exposed to hospitalization, recent trauma, or major surgeries, and who were on antipsychotic drugs before onset of renal disease were excluded. The Depression, Anxiety, and Stress Scale was used to assess the prevalence and level of depression, anxiety, and stress in hemodialysis patients. From the study population, 62.9% of patients had depression, 39.2% had stress, and 22.2% had anxiety. The patients with depression, anxiety, and stress had worse scores on all domains of the QoL questionnaire. Depression, anxiety, and stress were negatively correlated with quality of life.
接受血液透析的慢性肾病患者可能会面临一些问题，由于血液透析是一个耗时的过程，这增加了他们患上心理疾病的几率。本研究旨在评估精神疾病的发病率，并评估患者咨询对生活质量的影响。共有 135 名接受血液透析超过 3 个月的患者参与了这项研究。排除了住院、近期受过创伤或接受过大手术的患者，以及肾病发病前服用过抗精神病药物的患者。抑郁、焦虑和压力量表用于评估血液透析患者抑郁、焦虑和压力的发生率和程度。在研究人群中，62.9% 的患者患有抑郁症，39.2% 的患者患有压力症，22.2% 的患者患有焦虑症。患有抑郁症、焦虑症和压力症的患者在 QoL 问卷各方面的得分都较低。抑郁、焦虑和压力与生活质量呈负相关。
{"title":"Assessment of mental health and impact of patient counselling on quality of life of dialysis patients","authors":"Nahla Fathima, Nesniya T, Nivedhya Jith, Parvathy P S, Tamilselvan T, Rosemary Thomas","doi":"10.30574/gscbps.2024.26.3.0077","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.3.0077","url":null,"abstract":"Chronic renal disease patients undergoing hemodialysis may face several problems that increase their chance of developing psychological suffering as hemodialysis is a time-consuming process. This study was conducted to assess the prevalence of psychiatric illness and to evaluate the impact of patient counselling on the quality of life. A total of 135 patients undergoing hemodialysis for more than 3 months were enrolled in the study. Patients who were exposed to hospitalization, recent trauma, or major surgeries, and who were on antipsychotic drugs before onset of renal disease were excluded. The Depression, Anxiety, and Stress Scale was used to assess the prevalence and level of depression, anxiety, and stress in hemodialysis patients. From the study population, 62.9% of patients had depression, 39.2% had stress, and 22.2% had anxiety. The patients with depression, anxiety, and stress had worse scores on all domains of the QoL questionnaire. Depression, anxiety, and stress were negatively correlated with quality of life.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"50 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140362943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Spices are used globally, and are of great importance in indigenous culinary and traditional medicine applications, they are the product of dried fruits, seed root, bark or vegetative substances. This study synthesized food spice from Lippia multiflora, ginger and turmeric. Some anti-nutritional factors and the chemical composition of the extracted essential oils were evaluated. The chemical composition of the extracted essential oils revealed the presence of over ten (10) compounds, with 2-Pyrrolidinone, 1 methyl (35.21 %), Cyclo-hexane-carboxylic acid (24.00 %), Diethyl Phthalate (15.46 %) and 2-Pyrimidinamine,4,6-dimethyl (5.60 %) being the predominant compounds in the oil. The phytochemical screening of the food spice indicated the respective phytate, oxalate and tannin values for the food spice as; 0.71 mg/100 g, 1.2 mg/100 g and 0.51 mg/100 g respectively. These results are in agreement with previous studies where essential oils were shown to contain complex mixtures of compounds with varying chemical compositions.
{"title":"Extraction and characterization of essential oils from synthesized food spice (Lippia multiflora, ginger & turmeric)","authors":"Olukayode Adediran Okunade, Abiodun Olaniyi Fatoye","doi":"10.30574/gscbps.2024.26.3.0087","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.3.0087","url":null,"abstract":"Spices are used globally, and are of great importance in indigenous culinary and traditional medicine applications, they are the product of dried fruits, seed root, bark or vegetative substances. This study synthesized food spice from Lippia multiflora, ginger and turmeric. Some anti-nutritional factors and the chemical composition of the extracted essential oils were evaluated. The chemical composition of the extracted essential oils revealed the presence of over ten (10) compounds, with 2-Pyrrolidinone, 1 methyl (35.21 %), Cyclo-hexane-carboxylic acid (24.00 %), Diethyl Phthalate (15.46 %) and 2-Pyrimidinamine,4,6-dimethyl (5.60 %) being the predominant compounds in the oil. The phytochemical screening of the food spice indicated the respective phytate, oxalate and tannin values for the food spice as; 0.71 mg/100 g, 1.2 mg/100 g and 0.51 mg/100 g respectively. These results are in agreement with previous studies where essential oils were shown to contain complex mixtures of compounds with varying chemical compositions.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140363290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-30DOI: 10.30574/gscbps.2024.26.3.0101
Badrul Huda, Epy Muhammad Luqman, Suryo Kuncorojakti, Eka Pramyrtha Hestianah
Objective: This study aims to determine the effect of carbofuran exposure during the embryonic period on the histopathological images of the liver of one day old and two-week-old chicks after they hatch. Method: 60 embryonated chicken eggs from broiler parent stock with an average weight of 62.064 mg were divided into two observation groups: one day old chicks and two-week-old chicks after they hatched. Carbofuran was dissolved in aquabidest and then was injected into the egg yolk at a dose of 0.0106 mg/0.1 ml/egg (P1) and 0.0127 mg/0.1 ml/egg (P2). The control group (P0) was injected with 0.1 ml of aquabidest. All eggs were incubated in an electric incubator until they hatched. The livers of one day and two-week-old chicks were collected for HE staining. The variables observed were the level of liver cell necrosis in the central vein and Kiernan's triangle. Data were analyzed using Kruskal Wallis and it continued with multiple comparison tests. Results: The results showed that the carbofuran group of one day old chicks treated with at a dose of 0.0106 mg/0.1 ml/egg had a significant effect on necrosis of liver cells in the central vein and in Kiernan's triangle. Moreover,the carbofuran group of two week old chicks treated at a dose of 0.0127 mg/0.1 ml/egg (P2) and 0.0106 mg/0.1 ml/egg (P1) had a significant effect compared to the P0 group (p<0.05). Conclusion Carbofuran insecticide at a dose of 0.0127 mg/0.1 ml/egg was proven to cause the necrosis of liver cells in the central vein and in Kiernan triangle of one day and two-week-old chicks when they were exposed to carbofuran during the embryonic period.
{"title":"Histopathological liver of one day old and two-week-old chicks exposed to carbofuran during embryonic period","authors":"Badrul Huda, Epy Muhammad Luqman, Suryo Kuncorojakti, Eka Pramyrtha Hestianah","doi":"10.30574/gscbps.2024.26.3.0101","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.3.0101","url":null,"abstract":"Objective: This study aims to determine the effect of carbofuran exposure during the embryonic period on the histopathological images of the liver of one day old and two-week-old chicks after they hatch. Method: 60 embryonated chicken eggs from broiler parent stock with an average weight of 62.064 mg were divided into two observation groups: one day old chicks and two-week-old chicks after they hatched. Carbofuran was dissolved in aquabidest and then was injected into the egg yolk at a dose of 0.0106 mg/0.1 ml/egg (P1) and 0.0127 mg/0.1 ml/egg (P2). The control group (P0) was injected with 0.1 ml of aquabidest. All eggs were incubated in an electric incubator until they hatched. The livers of one day and two-week-old chicks were collected for HE staining. The variables observed were the level of liver cell necrosis in the central vein and Kiernan's triangle. Data were analyzed using Kruskal Wallis and it continued with multiple comparison tests. Results: The results showed that the carbofuran group of one day old chicks treated with at a dose of 0.0106 mg/0.1 ml/egg had a significant effect on necrosis of liver cells in the central vein and in Kiernan's triangle. Moreover,the carbofuran group of two week old chicks treated at a dose of 0.0127 mg/0.1 ml/egg (P2) and 0.0106 mg/0.1 ml/egg (P1) had a significant effect compared to the P0 group (p<0.05). Conclusion Carbofuran insecticide at a dose of 0.0127 mg/0.1 ml/egg was proven to cause the necrosis of liver cells in the central vein and in Kiernan triangle of one day and two-week-old chicks when they were exposed to carbofuran during the embryonic period.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"27 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140363365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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