Pub Date : 2024-02-28DOI: 10.30574/gscbps.2024.26.2.0054
Andrew Andrew Chimezie, Andrew Chimezie Andrew, R. Nwaoguikpe, Cosmas Onyekachi, Ujowundu Favour, Ntite Ujowundu
Justicia carnea is the largest genus of Acanthaceae and is a medicinal plant used widely in Nigeria, reported to have diverse functions, including blood-boosting potential. The phytochemicals, vitamins, minerals, amino acid and proximate compositions of the leaf extract of Justicia carnea were determined using standard methods. Results of the quantitative phytochemical analyses on the leaves of Justicia carnea recorded appreciable presence of flavonoids, alkaloids, saponins, tannins, cyanogenic glycosides, oxalate, and phytate using GC-FID. Ribalinidine with 42.08±0.03 mg/kg was the highest alkaloid, Flavan -3-ol was 21.18±0.02 mg/kg, presented the highest amount of flavonoids, and phytate with 25.69±0.07 mg/kg as the highest antinutrient. Analysis of Justicia carnea leaves recorded iron (8.61mg/kg) as the major elemental content followed by potassium (5.29±0.11mg/kg). The concentrations of vitamins in Justicia carnea leaves showed vitamin C, with the highest value of (232.32±12.26 mg/100g) followed by Vitamin A (22.16±2.12 mg/kg). The results of proximate composition on Justicia carnea leaves showed a high concentration of carbohydrate (60.35±3.05%) and appreciable amounts of ash (15.02±1.01%), fibre (9.29±0.93%), protein (8.40±0.41%), and low amount of lipid (1.50±0.09%). The amino acid results showed the presence of both essential and non-essential amino acids with their concentrations in increasing and decreasing orders. These results obtained showed that leaves of Justicia carnea may serve as rich source of natural antioxidants, this may be attributed to its rich phytochemicals, nutrients and vitamin compositions and could be recommended as a potential source of useful bioactive constituents as vegetable supplement and has no toxic effect and as an effective hematinic supplement when prepared adequately.
{"title":"Biochemical analysis of Justicia carnea leaves used as a hematinic","authors":"Andrew Andrew Chimezie, Andrew Chimezie Andrew, R. Nwaoguikpe, Cosmas Onyekachi, Ujowundu Favour, Ntite Ujowundu","doi":"10.30574/gscbps.2024.26.2.0054","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0054","url":null,"abstract":"Justicia carnea is the largest genus of Acanthaceae and is a medicinal plant used widely in Nigeria, reported to have diverse functions, including blood-boosting potential. The phytochemicals, vitamins, minerals, amino acid and proximate compositions of the leaf extract of Justicia carnea were determined using standard methods. Results of the quantitative phytochemical analyses on the leaves of Justicia carnea recorded appreciable presence of flavonoids, alkaloids, saponins, tannins, cyanogenic glycosides, oxalate, and phytate using GC-FID. Ribalinidine with 42.08±0.03 mg/kg was the highest alkaloid, Flavan -3-ol was 21.18±0.02 mg/kg, presented the highest amount of flavonoids, and phytate with 25.69±0.07 mg/kg as the highest antinutrient. Analysis of Justicia carnea leaves recorded iron (8.61mg/kg) as the major elemental content followed by potassium (5.29±0.11mg/kg). The concentrations of vitamins in Justicia carnea leaves showed vitamin C, with the highest value of (232.32±12.26 mg/100g) followed by Vitamin A (22.16±2.12 mg/kg). The results of proximate composition on Justicia carnea leaves showed a high concentration of carbohydrate (60.35±3.05%) and appreciable amounts of ash (15.02±1.01%), fibre (9.29±0.93%), protein (8.40±0.41%), and low amount of lipid (1.50±0.09%). The amino acid results showed the presence of both essential and non-essential amino acids with their concentrations in increasing and decreasing orders. These results obtained showed that leaves of Justicia carnea may serve as rich source of natural antioxidants, this may be attributed to its rich phytochemicals, nutrients and vitamin compositions and could be recommended as a potential source of useful bioactive constituents as vegetable supplement and has no toxic effect and as an effective hematinic supplement when prepared adequately.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"33 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140418773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.30574/gscbps.2024.26.2.0051
Celine Chelsea Hartono, Janti Sudiono, Pretty Trisfilha
Background: The seed coat of Borassus flabellifer L. fruit contains antioxidants that can neutralize the toxic effects of free radicals and can fight DNA damage due to exposure to oxidative stress. The extract of B. flabellifer is proven to have cytotoxic effects and can inhibit the growth of several types of cancer cells such as HeLa cell lines. Objective: To determine the cytotoxicity effect of extract of B. flabellifer L. fruit seed coat against HSC-3 cell line. Methods: Laboratory experimental research was conducted using HSC-3 cell lines treated with B. flabellifer L. fruit seed coat extract at concentrations of 93.75 µg/mL, 187.5 µg/mL, 375 µg/mL, 750 µg/mL, and 1,500 µg/mL, negative control DMEM-FBS 20%, and positive control anti cancer drugs Doxorubicin 3 µM. HSC-3 cell line cytotoxicity assay was performed using CCK-8 reagent. Micro plate reader at 450 nm wavelength was used to determine the viability of HSC-3 cell lines by measuring the optical density of formazan. Results: B. flabellifer fruit L. seed coat extract was able to reduce viability and was cytotoxic to HSC-3 cell lines at concentrations of 93.75 µg/mL, 187.5 µg/mL, 375 µg/mL, 750 µg/mL, and 1,500 µg/mL, with an IC50 value of 141.9 µg/mL. Conclusion: B. flabellifer L. fruit seed coat extract can reduce the ability of HSC-3 cell line cancer cells to survive by inhibiting cell proliferation due to its cytotoxicity effect.
背景：Borassus flabellifer L.果实的种皮中含有抗氧化剂，可以中和自由基的毒性作用，并能对抗因暴露于氧化应激而造成的 DNA 损伤。经证实，花叶鲍鱼提取物具有细胞毒性作用，可抑制几种癌细胞（如 HeLa 细胞系）的生长。研究目的测定 B. flabellifer L. 果实种皮提取物对 HSC-3 细胞系的细胞毒性作用。方法：以 HSC-3 细胞系为研究对象，进行实验室实验研究：使用浓度为 93.75 µg/mL、187.5 µg/mL、375 µg/mL、750 µg/mL 和 1,500 µg/mL 的 B. flabellifer L. 果实种皮提取物处理 HSC-3 细胞系，阴性对照 DMEM-FBS 20%，阳性对照抗癌药物多柔比星 3 µM。使用 CCK-8 试剂对 HSC-3 细胞株进行细胞毒性检测。使用波长为 450 nm 的微孔平板阅读器通过测量甲状腺素的光密度来确定 HSC-3 细胞株的存活率。结果在浓度为 93.75 微克/毫升、187.5 微克/毫升、375 微克/毫升、750 微克/毫升和 1,500 微克/毫升时，B. flabellifer 果实 L. 种皮提取物能够降低 HSC-3 细胞株的活力并具有细胞毒性，IC50 值为 141.9 微克/毫升。结论B. flabellifer L.果实种皮提取物的细胞毒性作用可抑制细胞增殖，从而降低 HSC-3 细胞系癌细胞的存活能力。
{"title":"Effects of Borassus flabellifer L. fruit seed coat on HSC-3 cell line","authors":"Celine Chelsea Hartono, Janti Sudiono, Pretty Trisfilha","doi":"10.30574/gscbps.2024.26.2.0051","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0051","url":null,"abstract":"Background: The seed coat of Borassus flabellifer L. fruit contains antioxidants that can neutralize the toxic effects of free radicals and can fight DNA damage due to exposure to oxidative stress. The extract of B. flabellifer is proven to have cytotoxic effects and can inhibit the growth of several types of cancer cells such as HeLa cell lines. Objective: To determine the cytotoxicity effect of extract of B. flabellifer L. fruit seed coat against HSC-3 cell line. Methods: Laboratory experimental research was conducted using HSC-3 cell lines treated with B. flabellifer L. fruit seed coat extract at concentrations of 93.75 µg/mL, 187.5 µg/mL, 375 µg/mL, 750 µg/mL, and 1,500 µg/mL, negative control DMEM-FBS 20%, and positive control anti cancer drugs Doxorubicin 3 µM. HSC-3 cell line cytotoxicity assay was performed using CCK-8 reagent. Micro plate reader at 450 nm wavelength was used to determine the viability of HSC-3 cell lines by measuring the optical density of formazan. Results: B. flabellifer fruit L. seed coat extract was able to reduce viability and was cytotoxic to HSC-3 cell lines at concentrations of 93.75 µg/mL, 187.5 µg/mL, 375 µg/mL, 750 µg/mL, and 1,500 µg/mL, with an IC50 value of 141.9 µg/mL. Conclusion: B. flabellifer L. fruit seed coat extract can reduce the ability of HSC-3 cell line cancer cells to survive by inhibiting cell proliferation due to its cytotoxicity effect.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"26 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140422142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.30574/gscbps.2024.26.2.0062
Généviève ZABRE, Elisabeth OUEDRAOGO, Joëlle W.R. YOUGBARE, Armand S.S. Nacoulma, Adama Kabore, Balé BAYALA
In Burkina Faso, the production of small ruminants is confronted with many constraints, including gastrointestinal parasitosis. These parasitic diseases causes many mortality in small ruminant breeding therefore considerable economic losses. Thus, the present study was carried out with the aim of evaluating the in vitro anthelmintic effectiveness of Azadiracta indica (A. indica) leaves on two life stages of Haemonchus contortus (H. contortus): eggs and adult worms. To do this, an aqueous and ethanolic extract of A. indica leaves was carried out. From these extracts, five (5) increasing concentrations were performed: 1.25; 2.5; 5 ; 10 and 20 mg/mL and three (3) controls (distilled water, DMSO 2% and albendazol). These concentrations were brought into contact with i) eggs for Eggs Hatch inhibition Assay st (EHA) and ii) Adult Mortality Test (AMT). Three replicates were performed for each experimental preparations. In vitro observations showed that ethanolic extract of A. indica caused high mortalities (p < 0.0001) ranging from 44.44 to 100% at 2 hours and 6 hours of exposure respectively at 20 mg/mL. In the same time interval, albendazol showed 100% of mortality in H. contortus. For EHA, the inhibition rate varied from 18% to 85% for the aqueous extract and from 54% to 93% for ethanolic extract. We can therefore say that A. indica extracts have nematicidal and ovicidal effect.
{"title":"Comparative study of aqueous and alcoholic extract of Azadirachta indica A. Juss. (Meliaceae) leaves on two life stages of Haemonchus contortu","authors":"Généviève ZABRE, Elisabeth OUEDRAOGO, Joëlle W.R. YOUGBARE, Armand S.S. Nacoulma, Adama Kabore, Balé BAYALA","doi":"10.30574/gscbps.2024.26.2.0062","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0062","url":null,"abstract":"In Burkina Faso, the production of small ruminants is confronted with many constraints, including gastrointestinal parasitosis. These parasitic diseases causes many mortality in small ruminant breeding therefore considerable economic losses. Thus, the present study was carried out with the aim of evaluating the in vitro anthelmintic effectiveness of Azadiracta indica (A. indica) leaves on two life stages of Haemonchus contortus (H. contortus): eggs and adult worms. To do this, an aqueous and ethanolic extract of A. indica leaves was carried out. From these extracts, five (5) increasing concentrations were performed: 1.25; 2.5; 5 ; 10 and 20 mg/mL and three (3) controls (distilled water, DMSO 2% and albendazol). These concentrations were brought into contact with i) eggs for Eggs Hatch inhibition Assay st (EHA) and ii) Adult Mortality Test (AMT). Three replicates were performed for each experimental preparations. In vitro observations showed that ethanolic extract of A. indica caused high mortalities (p < 0.0001) ranging from 44.44 to 100% at 2 hours and 6 hours of exposure respectively at 20 mg/mL. In the same time interval, albendazol showed 100% of mortality in H. contortus. For EHA, the inhibition rate varied from 18% to 85% for the aqueous extract and from 54% to 93% for ethanolic extract. We can therefore say that A. indica extracts have nematicidal and ovicidal effect.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"47 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140418985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.30574/gscbps.2024.26.2.0061
Jeannoda Victor Louis, Voara Randriamanantoa-Arisoa, Mihajasoa Stella Razanatseheno, L. Randriamampianina, Zara Nomentsoa Razafiarimanga, Huchard Paul, Bertin Randrianirainy, Hanitra Ranjàna Randrianarivo, Danielle Aurore, Doll Rakoto-Ranoromalala, R. Rakotosaona, J. O. Andrianaivoravelona, V. Jeannoda
The present work aims to evaluate the chemical composition, the antimicrobial and antioxidant properties of the essential oil from Cryptocarya ovalifolia van der Werff (COEO) leaves collected in Mandraka forest. COEO was extracted by steam distillation from fresh leaves, with a yield of 0.7%. COEO appeared as a clear liquid, yellow, with a pleasant odour and relative density of 0.8943 at 20 °C, a refractive index of 1.4746, an optical rotation of – 31°13, an acid index of 0.638 and an ester index of 6.375. A total of 31 constituents accounting for 99.98% of the total essential oil content were identified by gas chromatography/mass spectrometry. The main component was sabinene (30.65%) and the major components (>5%) were limonene (17.33%), methyleugenol (14%) and α-pinene (8.33%). The antibacterial activity was tested on nine pathogenic microorganisms including four Gram positive bacteria, four Gram negative bacteria and one yeast using the disk diffusion and the microdilution assays. COEO was active on 4 strains with inhibition zone diameter (IZD) ranging from 8.7 mm (Bacillus cereus) to 12.7 mm (Clostridium perfringens). Minimum Inhibitory Concentration (MIC) varied from 1.12 to 2.23 mg/ml and Minimum Bactericidal Concentration (MBC) was 8.93 mg/ml for all strains. COEO had bacteriostatic action on Streptococcus pneumoniae, Clostridium perfringens, Bacillus cereus and Candida albicans (MBC/MIC>4) and bactericidal action on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Vibrio fischeri (MBC/MIC≤4). COEO exhibited radical scavenging activity against DPPH with an IC50 of 123.29 mg/ml. By oral route, its LD50 was 1.9/Kg body weight.
{"title":"Determination of the chemical composition and evaluation of the antimicrobial and antioxidant potential of the essential oil from Cryptocarya ovalifolia van der Werff leaves (Lauraceae)","authors":"Jeannoda Victor Louis, Voara Randriamanantoa-Arisoa, Mihajasoa Stella Razanatseheno, L. Randriamampianina, Zara Nomentsoa Razafiarimanga, Huchard Paul, Bertin Randrianirainy, Hanitra Ranjàna Randrianarivo, Danielle Aurore, Doll Rakoto-Ranoromalala, R. Rakotosaona, J. O. Andrianaivoravelona, V. Jeannoda","doi":"10.30574/gscbps.2024.26.2.0061","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0061","url":null,"abstract":"The present work aims to evaluate the chemical composition, the antimicrobial and antioxidant properties of the essential oil from Cryptocarya ovalifolia van der Werff (COEO) leaves collected in Mandraka forest. COEO was extracted by steam distillation from fresh leaves, with a yield of 0.7%. COEO appeared as a clear liquid, yellow, with a pleasant odour and relative density of 0.8943 at 20 °C, a refractive index of 1.4746, an optical rotation of – 31°13, an acid index of 0.638 and an ester index of 6.375. A total of 31 constituents accounting for 99.98% of the total essential oil content were identified by gas chromatography/mass spectrometry. The main component was sabinene (30.65%) and the major components (>5%) were limonene (17.33%), methyleugenol (14%) and α-pinene (8.33%). The antibacterial activity was tested on nine pathogenic microorganisms including four Gram positive bacteria, four Gram negative bacteria and one yeast using the disk diffusion and the microdilution assays. COEO was active on 4 strains with inhibition zone diameter (IZD) ranging from 8.7 mm (Bacillus cereus) to 12.7 mm (Clostridium perfringens). Minimum Inhibitory Concentration (MIC) varied from 1.12 to 2.23 mg/ml and Minimum Bactericidal Concentration (MBC) was 8.93 mg/ml for all strains. COEO had bacteriostatic action on Streptococcus pneumoniae, Clostridium perfringens, Bacillus cereus and Candida albicans (MBC/MIC>4) and bactericidal action on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Vibrio fischeri (MBC/MIC≤4). COEO exhibited radical scavenging activity against DPPH with an IC50 of 123.29 mg/ml. By oral route, its LD50 was 1.9/Kg body weight.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140420023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.30574/gscbps.2024.26.2.0065
Emeka Sabastine Asiwe, Prince Chimaobi Ejiogu, Uchechi Emmanuela Enenebeaku, Peter Onyedikachi Opara, Obinna Michael Oguoma
This study determined the alterations in haematological parameters of indomethacin-ulcerated rats treated with P. americana seed (PAS) and B. pinnatum leaf (BPL) ethyl acetate fraction. Fifty (50) healthy Wistar albino rats were randomly assigned into ten (10) groups of five (5) animals each according to body weight (100-120 g). Animals in group I served as normal control; the rats in groups III-X were pre-treated with 20 mgKg-1 b. wt. Omeprazole (STD), 400 mgKg-1 b. wt. PAS, 400 mgKg-1 b. wt. BPL, 400 mgKg-1 b. wt. PAS + BPL (1:1), 400 mgKg-1 b. wt. PAS + BPL (1:2), 400 mgKg-1 b. wt. PAS + BPL (1:3) respectively for 21 days. Thereafter, animals in groups II-X were induced for gastric ulcer by intubation of 30 mgKg-1 b. wt. indomethacin after being fasted for 24 hours. The animals were sacrificed after 4 hours and the complete blood count was determined using an automated hematology analyzer. Results obtained from the study showed significant (p<0.05) elevation in the total and differential white blood cell count of Wistar rats in the indomethacin-induced gastric ulcer control group and ethyl acetate fraction treated groups. Indomethacin, PAS, and BPL ethyl acetate fraction and the combinations did not cause marked changes in red blood cell indices. However, platelet count was significantly (p<0.05) reduced by the combinations of the P. americana seed and B. pinnatum leaf ethyl acetate fraction. These findings demonstrated that indomethacin induction resulted in a significant increase in the total white blood cell and platelet count when matched with the control group; this was similarly seen in PAS+BPL (1:3) and PAS+BPL (3:1) combinations of PAS and BPL. The result is suggestive of a compromise in the hemostatic capability of the blood in rats treated with some combinations of P. americana seed and B. pinnatum leaf ethyl acetate fraction.
本研究确定了吲哚美辛溃疡大鼠血液学参数的变化，这些参数是用美洲大鼠种子（PAS）和羽扇豆叶（BPL）乙酸乙酯馏分处理后得出的。将 50 只健康的 Wistar 白化大鼠按体重（100-120 克）随机分为 10 组，每组 5 只。I 组大鼠为正常对照组；III-X 组大鼠分别用 20 mgKg-1 b. wt. 奥美拉唑（STD）、400 mgKg-1 b. wt.PAS、400 mgKg-1 b. wt. BPL、400 mgKg-1 b. wt. PAS + BPL (1:1)、400 mgKg-1 b. wt. PAS + BPL (1:2)、400 mgKg-1 b. wt. PAS + BPL (1:3)，分别治疗 21 天。之后，禁食 24 小时后，给 II-X 组动物灌胃 30 mgKg-1 b. wt. 的吲哚美辛，诱发胃溃疡。4 小时后动物被处死，并使用自动血液分析仪测定全血细胞计数。研究结果表明，在吲哚美辛诱发胃溃疡对照组和乙酸乙酯馏分处理组中，Wistar 大鼠的白细胞总数和差值均有显著升高（p<0.05）。吲哚美辛、PAS 和 BPL 乙酸乙酯馏分以及它们的组合并没有引起红细胞指数的明显变化。然而，美洲商陆种子和羽扇豆叶乙酸乙酯馏分的组合会显著降低血小板计数（p<0.05）。这些研究结果表明，与对照组相比，吲哚美辛诱导可使白细胞总数和血小板计数显著增加；这在 PAS+BPL (1:3) 和 PAS+BPL (3:1) 的 PAS 和 BPL 组合中也同样可见。这一结果表明，用美洲大鼠种子和羽扇豆叶乙酸乙酯馏分的某些组合处理大鼠，其血液止血能力会受到影响。
{"title":"Alterations in haematological indices of indomethacin-ulcerated rats treated with Persea americana seed and Bryophyllum pinnatum leaf ethyl acetate fraction","authors":"Emeka Sabastine Asiwe, Prince Chimaobi Ejiogu, Uchechi Emmanuela Enenebeaku, Peter Onyedikachi Opara, Obinna Michael Oguoma","doi":"10.30574/gscbps.2024.26.2.0065","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0065","url":null,"abstract":"This study determined the alterations in haematological parameters of indomethacin-ulcerated rats treated with P. americana seed (PAS) and B. pinnatum leaf (BPL) ethyl acetate fraction. Fifty (50) healthy Wistar albino rats were randomly assigned into ten (10) groups of five (5) animals each according to body weight (100-120 g). Animals in group I served as normal control; the rats in groups III-X were pre-treated with 20 mgKg-1 b. wt. Omeprazole (STD), 400 mgKg-1 b. wt. PAS, 400 mgKg-1 b. wt. BPL, 400 mgKg-1 b. wt. PAS + BPL (1:1), 400 mgKg-1 b. wt. PAS + BPL (1:2), 400 mgKg-1 b. wt. PAS + BPL (1:3) respectively for 21 days. Thereafter, animals in groups II-X were induced for gastric ulcer by intubation of 30 mgKg-1 b. wt. indomethacin after being fasted for 24 hours. The animals were sacrificed after 4 hours and the complete blood count was determined using an automated hematology analyzer. Results obtained from the study showed significant (p<0.05) elevation in the total and differential white blood cell count of Wistar rats in the indomethacin-induced gastric ulcer control group and ethyl acetate fraction treated groups. Indomethacin, PAS, and BPL ethyl acetate fraction and the combinations did not cause marked changes in red blood cell indices. However, platelet count was significantly (p<0.05) reduced by the combinations of the P. americana seed and B. pinnatum leaf ethyl acetate fraction. These findings demonstrated that indomethacin induction resulted in a significant increase in the total white blood cell and platelet count when matched with the control group; this was similarly seen in PAS+BPL (1:3) and PAS+BPL (3:1) combinations of PAS and BPL. The result is suggestive of a compromise in the hemostatic capability of the blood in rats treated with some combinations of P. americana seed and B. pinnatum leaf ethyl acetate fraction.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"28 S12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140423038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.30574/gscbps.2024.26.2.0067
Nisma Douzi, Amina Himri, Imad-Eddine El khamlichi, Oussama Grari, Soufiane Beyyoudh, Sabah Mokhtari, Dounia El Moujtahide, El-houcine Sebbar, Mohammed Choukri
The aim of our work was to evaluate the analytical performance of CA 125 determination by a two-step immunoassay using microparticle chemiluminescence immunoassay (CMIA) technology, in accordance with the Scope A criteria of the guide of the verification/validation of medical biology methods. We evaluated the repeatability and the intermediate precision of the CA 125 assay.The results obtained are very satisfactory for the three levels (low, medium and high), both for intermediate fidelity, with coefficients of variation (CV) of 2.68%, 1.62% and 2.11% respectively, and for repeatability, with coefficients of variation of CV1 = 2.17%, CV2 = 2.04%, and CV3 = 1.38% respectively. The results obtained made it possible to verify the method's performance and compare it with the analytical objectives set in order to meet the regulatory and normative requirements set by the supplier and learned societies. The achieved results facilitated the verification of the method's performance and its comparison with the analytical objectives established, aligning with regulatory and normative requirements outlined by the supplier and relevant professional learned societies.
我们的工作旨在根据医学生物学方法验证/确认指南的范围 A 标准，评估使用微粒子化学发光免疫测定（CMIA）技术的两步免疫测定法测定 CA 125 的分析性能。我们评估了 CA 125 检测法的可重复性和中间精密度。无论是中间精密度（变异系数（CV）分别为 2.68%、1.62% 和 2.11%）还是可重复性（变异系数分别为 CV1 = 2.17%、CV2 = 2.04% 和 CV3 = 1.38%），三个级别（低、中、高）的结果都非常令人满意。所获得的结果可以验证该方法的性能，并将其与设定的分析目标进行比较，以满足供应商和学术团体设定的监管和规范要求。所取得的结果有助于验证该方法的性能，并将其与既定的分析目标进行比较，以符合供应商和相关专业学会提出的监管和规范要求。
{"title":"Verification of the analytical performance of the serum CA 125 assay on the Abbott Alinity ci®: Experience of the biochemistry laboratory of the Mohammed VI university hospital of Oujda","authors":"Nisma Douzi, Amina Himri, Imad-Eddine El khamlichi, Oussama Grari, Soufiane Beyyoudh, Sabah Mokhtari, Dounia El Moujtahide, El-houcine Sebbar, Mohammed Choukri","doi":"10.30574/gscbps.2024.26.2.0067","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0067","url":null,"abstract":"The aim of our work was to evaluate the analytical performance of CA 125 determination by a two-step immunoassay using microparticle chemiluminescence immunoassay (CMIA) technology, in accordance with the Scope A criteria of the guide of the verification/validation of medical biology methods. We evaluated the repeatability and the intermediate precision of the CA 125 assay.The results obtained are very satisfactory for the three levels (low, medium and high), both for intermediate fidelity, with coefficients of variation (CV) of 2.68%, 1.62% and 2.11% respectively, and for repeatability, with coefficients of variation of CV1 = 2.17%, CV2 = 2.04%, and CV3 = 1.38% respectively. The results obtained made it possible to verify the method's performance and compare it with the analytical objectives set in order to meet the regulatory and normative requirements set by the supplier and learned societies. The achieved results facilitated the verification of the method's performance and its comparison with the analytical objectives established, aligning with regulatory and normative requirements outlined by the supplier and relevant professional learned societies.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"43 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140419023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.30574/gscbps.2024.26.2.0036
Olamide Durodola, Obianuju Opara, Uchechukwu Anthony Ogwurumba, Amarachukwu Bernaldine Isiaka, Ugonna Henry Uzoka, Temitayo Margaret Omoyeni, Sani Muhammad Sade, Blessing Helen Udoma, Mu’awiya Baba Aminu
Finding the phytochemical components of Cola nitida seeds and assessing the extracts' antibacterial properties against Salmonella typhi and E. coli were the main objectives of the current investigation. The bacteria (Salmonella typhi and E. coli) were taken from Usmanu Danfodiyo University's Teaching Hospital in Sokoto and were confirmed by a biochemical test. The extract of bitter kola (Cola nitida) was gathered from the Sokoto Old Market, Sokoto South Local Government, Sokoto. the flavonoids, tannins, glycosides, alkaloids, cardiac glycosides, steroid, volatile oil, balsam, and saponin glycosides that make up phytochemicals. The Agar well diffusion method was utilised to assess the test bacteria's sensitivity to the extracts. The concentrations of minimum bactericidal concentration (MBC) and minimum inhibition concentration (MIC) were ascertained. The result of this study has shown that the extract of Bitter kola (Cola nitida) contained phytochemical components. Components at high concentration include glycosides, alkaloids and volatile oil. While the components observed at moderate concentration includes tannins, flavonoids, saponins, steroids and balsams. The trace/low concentration component is cardiac glycosides. The result of the antibacterial activity has shown that methanolic extracts of Cola nitida seeds had a range zone of inhibition from 27.0mm to 19.5 mm at 150 mg/l -50mg/l concentration against Salmonella typhi, which shows a great zone of inhibition very close to the control antibiotics, while E. coli had inhibition zone from 19.0 -12.0 mg/l at 150 – 50 mg/l which shows moderate inhibition zone. All the tested strain shows a definite MIC and MBC activity which ranges from 8.5 to 3.5 mg/ml. The result confirmed the antibacterial activity of Cola nitida
{"title":"Health and environmental benefits of phytochemicals and antibacterial effectiveness of Cola nitida seed extracts on Salmonella typhi and Escherichia coli","authors":"Olamide Durodola, Obianuju Opara, Uchechukwu Anthony Ogwurumba, Amarachukwu Bernaldine Isiaka, Ugonna Henry Uzoka, Temitayo Margaret Omoyeni, Sani Muhammad Sade, Blessing Helen Udoma, Mu’awiya Baba Aminu","doi":"10.30574/gscbps.2024.26.2.0036","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0036","url":null,"abstract":"Finding the phytochemical components of Cola nitida seeds and assessing the extracts' antibacterial properties against Salmonella typhi and E. coli were the main objectives of the current investigation. The bacteria (Salmonella typhi and E. coli) were taken from Usmanu Danfodiyo University's Teaching Hospital in Sokoto and were confirmed by a biochemical test. The extract of bitter kola (Cola nitida) was gathered from the Sokoto Old Market, Sokoto South Local Government, Sokoto. the flavonoids, tannins, glycosides, alkaloids, cardiac glycosides, steroid, volatile oil, balsam, and saponin glycosides that make up phytochemicals. The Agar well diffusion method was utilised to assess the test bacteria's sensitivity to the extracts. The concentrations of minimum bactericidal concentration (MBC) and minimum inhibition concentration (MIC) were ascertained. The result of this study has shown that the extract of Bitter kola (Cola nitida) contained phytochemical components. Components at high concentration include glycosides, alkaloids and volatile oil. While the components observed at moderate concentration includes tannins, flavonoids, saponins, steroids and balsams. The trace/low concentration component is cardiac glycosides. The result of the antibacterial activity has shown that methanolic extracts of Cola nitida seeds had a range zone of inhibition from 27.0mm to 19.5 mm at 150 mg/l -50mg/l concentration against Salmonella typhi, which shows a great zone of inhibition very close to the control antibiotics, while E. coli had inhibition zone from 19.0 -12.0 mg/l at 150 – 50 mg/l which shows moderate inhibition zone. All the tested strain shows a definite MIC and MBC activity which ranges from 8.5 to 3.5 mg/ml. The result confirmed the antibacterial activity of Cola nitida","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140421214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Myriads of research reviews have justified that unrestricted and uncontrolled exposure to heavy metal contamination through occupation and environmental pollution generally result in health risks with attendant health burdens including genetic birth defects. This study evaluates the anticlastogenic potential of Euphorbia heterophylla leaf extract against lead-induced polychromatic erythrocytes and the damaged tissues of the albino rats. A total of sixty (60) albino rats were segregated into three sets namely Set I, II and III. Each set contains twenty albino rats which were then divided into four groups A, B, C and D containing five rats each. Group A serves as control and were treated with distilled water only. Group B rats received 2.5 mg/kg lead acetate while group C rats were simultaneously fed 25, 50 and 100 mg/kg Euphorbia heterophylla leaf extract concentrations and 2.5 mg/kg lead acetate at ratio 1:1. Rats in group D were administered 25, 50, and 100 mg/kg Euphorbia heterophylla leaf extract. The schedule of the animal treatment was divided into three set I, II and III while micronucleus assay and histopathological examination were carried out on the test animals. Results show that polychromatic erythrocytes induced by lead acetate was significantly (P<0.05) reduced in group C animals (6%), while lead acetate administered to animals in group B was highly clastogenic. Besides, the histopathological architecture of the rat tissues indicates protection offered by the extract against tissue atrophy.
大量研究综述证明，通过职业和环境污染无限制、无节制地接触重金属污染通常会导致健康风险和随之而来的健康负担，包括遗传性先天缺陷。本研究评估了大叶女贞叶提取物对铅诱导的多色红细胞和白化大鼠受损组织的抗衰老潜力。共有六十（60）只白化大鼠被分成三组，即第一组、第二组和第三组。每组包括 20 只白化大鼠，然后分为 A、B、C 和 D 四组，每组 5 只。A 组为对照组，只用蒸馏水处理。B 组大鼠摄入 2.5 毫克/千克醋酸铅，C 组大鼠同时摄入 25、50 和 100 毫克/千克大叶女贞叶提取物浓度和 2.5 毫克/千克醋酸铅，比例为 1:1。给 D 组大鼠喂食 25、50 和 100 毫克/千克大叶女贞叶提取物。动物治疗时间表分为 I、II 和 III 三组，同时对试验动物进行微核试验和组织病理学检查。结果表明，醋酸铅诱导的多色红细胞在 C 组动物中明显减少（P<0.05）（6%），而 B 组动物的醋酸铅具有高度致突变性。此外，大鼠组织的组织病理学结构表明，萃取物能防止组织萎缩。
{"title":"Anticlastogenic potential of euphorbia heterophylla leaf extract against lead-induced polychromatic erythrocytes and tissue atrophy","authors":"Oyewumi Nather Oyewole, Ifeoluwa Lois Onifade, Folasade Bosede Oluwatobi, Oladimeji Samuel Tugbobo","doi":"10.30574/gscbps.2024.26.2.0050","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0050","url":null,"abstract":"Myriads of research reviews have justified that unrestricted and uncontrolled exposure to heavy metal contamination through occupation and environmental pollution generally result in health risks with attendant health burdens including genetic birth defects. This study evaluates the anticlastogenic potential of Euphorbia heterophylla leaf extract against lead-induced polychromatic erythrocytes and the damaged tissues of the albino rats. A total of sixty (60) albino rats were segregated into three sets namely Set I, II and III. Each set contains twenty albino rats which were then divided into four groups A, B, C and D containing five rats each. Group A serves as control and were treated with distilled water only. Group B rats received 2.5 mg/kg lead acetate while group C rats were simultaneously fed 25, 50 and 100 mg/kg Euphorbia heterophylla leaf extract concentrations and 2.5 mg/kg lead acetate at ratio 1:1. Rats in group D were administered 25, 50, and 100 mg/kg Euphorbia heterophylla leaf extract. The schedule of the animal treatment was divided into three set I, II and III while micronucleus assay and histopathological examination were carried out on the test animals. Results show that polychromatic erythrocytes induced by lead acetate was significantly (P<0.05) reduced in group C animals (6%), while lead acetate administered to animals in group B was highly clastogenic. Besides, the histopathological architecture of the rat tissues indicates protection offered by the extract against tissue atrophy.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"323 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140417381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.30574/gscbps.2024.26.2.0053
Ivan Kindekov, Liliya Grahlyova, Nina Petkova, Antoniya Nedeva
In the current research the attention is focused on the possibilities of identifying a hyperdiploidy myeloma clone (HdMC) by using a triple-color fluorescence “in situ” hybridization (FISH) probe. The cytogenetic results from the bone marrow aspirates of 26 patients with newly diagnosed multiple myeloma admitted in the Hematology Department of our hospital during the period from March to September 2023 have been analyzed. The group consists of 12 female and 14 male patients with an average age of 67 years. A FISH probe for establishing hyperdiploidy myeloma clone was used as well as the most common methods for detecting genetic aberrations affecting the long and short arms of the 1 and 14q32 chromosome rearrangements. According to the results, two subgroups of patients have been established. The first subgroup consists of the patients with positive FISH probes for hyperdiploidy myeloma and/or 14q34 rearrangements and 1q25/1p36, while the second one consists of patients, negative for all the three probes listed above. A comparison between the demographic, laboratory data and the ISS (International Staging System) stage of the two subgroups has been made. The collected data suggests that the use of the triple-color FISH probe, as well as some other factors in the analyzed information, increases the probability of detecting a HdMC by 23%.
{"title":"Нyperdiploid мultiple мyeloma-cytogenetic and clinical aspects","authors":"Ivan Kindekov, Liliya Grahlyova, Nina Petkova, Antoniya Nedeva","doi":"10.30574/gscbps.2024.26.2.0053","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.2.0053","url":null,"abstract":"In the current research the attention is focused on the possibilities of identifying a hyperdiploidy myeloma clone (HdMC) by using a triple-color fluorescence “in situ” hybridization (FISH) probe. The cytogenetic results from the bone marrow aspirates of 26 patients with newly diagnosed multiple myeloma admitted in the Hematology Department of our hospital during the period from March to September 2023 have been analyzed. The group consists of 12 female and 14 male patients with an average age of 67 years. A FISH probe for establishing hyperdiploidy myeloma clone was used as well as the most common methods for detecting genetic aberrations affecting the long and short arms of the 1 and 14q32 chromosome rearrangements. According to the results, two subgroups of patients have been established. The first subgroup consists of the patients with positive FISH probes for hyperdiploidy myeloma and/or 14q34 rearrangements and 1q25/1p36, while the second one consists of patients, negative for all the three probes listed above. A comparison between the demographic, laboratory data and the ISS (International Staging System) stage of the two subgroups has been made. The collected data suggests that the use of the triple-color FISH probe, as well as some other factors in the analyzed information, increases the probability of detecting a HdMC by 23%.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"18 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140418685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.30574/gscbps.2024.26.1.0024
Loubna Yacoubi, Mouad Harandou, Amina Himri, Zainab Kajeiou, Sabah Mokhtari, Issam Mokhtari, Nisma Douzi, El houcine Sebbar, Mohammed Choukri
Introduction: The aim of our study is to evaluate the haptoglobin assay on the Alinity ci automated system. This evaluation is part of an overall approach to verifying the methods used in the central laboratory of the Mohammed VI University Hospital of Oujda , with a view to compiling an accreditation file in accordance with the requirements of standard NF ISO 15189. Materials and methods: The working methodology adapted by our study is based on the recommendations of the protocol of the COFRAC accreditation technical guide SH GTA 04. Verification involved assessing the repeatability and reproducibility of Alinity ci . Results: The results obtained for the various haptoglobin assay verification criteria show satisfactory repeatability for all three levels, with CV1=1.10%, CV2=0.78% and CV3=1.07% respectively. intra-laboratory reproducibility was satisfactory for all three levels, with CV1=1.79%, CV2= 4.2% and CV3=1.62% respectively. Discussion: Verification of an analytical method is an essential step in guaranteeing that the result obtained is as close as possible to the reference value of a sample. Comparing our results with the CV adopted by the SFBC, we can see that the results are in line with and below the tolerated limits. Conclusion: We can therefore conclude that the Abbott Alinity ci system meets the requirements set by scientific societies for the determination of haptoglobin.
简介我们的研究旨在对 Alinity ci 自动化系统上的血红蛋白检测方法进行评估。该评估是验证乌季达穆罕默德六世大学医院中心实验室所用方法的整体方法的一部分，目的是根据 NF ISO 15189 标准的要求编制一份认证文件。材料和方法：我们的研究采用的工作方法是基于法国食品和药品委员会认证技术指南 SH GTA 04 协议的建议。验证包括评估 Alinity ci 的可重复性和再现性。结果：对各种血红蛋白检测验证标准的结果显示，所有三个级别的重复性都令人满意，CV1=1.10%，CV2=0.78%，CV3=1.07%。讨论分析方法的验证是保证所得结果尽可能接近样品参考值的重要步骤。将我们的结果与 SFBC 采用的 CV 值进行比较，我们可以看到结果符合并低于容许限度。结论因此，我们可以得出结论：雅培 Alinity ci 系统符合科学协会对测定血红蛋白的要求。
{"title":"Verification of the analytical performance of the serum haptoglobin assay on the Abbott Alinity ci","authors":"Loubna Yacoubi, Mouad Harandou, Amina Himri, Zainab Kajeiou, Sabah Mokhtari, Issam Mokhtari, Nisma Douzi, El houcine Sebbar, Mohammed Choukri","doi":"10.30574/gscbps.2024.26.1.0024","DOIUrl":"https://doi.org/10.30574/gscbps.2024.26.1.0024","url":null,"abstract":"Introduction: The aim of our study is to evaluate the haptoglobin assay on the Alinity ci automated system. This evaluation is part of an overall approach to verifying the methods used in the central laboratory of the Mohammed VI University Hospital of Oujda , with a view to compiling an accreditation file in accordance with the requirements of standard NF ISO 15189. Materials and methods: The working methodology adapted by our study is based on the recommendations of the protocol of the COFRAC accreditation technical guide SH GTA 04. Verification involved assessing the repeatability and reproducibility of Alinity ci . Results: The results obtained for the various haptoglobin assay verification criteria show satisfactory repeatability for all three levels, with CV1=1.10%, CV2=0.78% and CV3=1.07% respectively. intra-laboratory reproducibility was satisfactory for all three levels, with CV1=1.79%, CV2= 4.2% and CV3=1.62% respectively. Discussion: Verification of an analytical method is an essential step in guaranteeing that the result obtained is as close as possible to the reference value of a sample. Comparing our results with the CV adopted by the SFBC, we can see that the results are in line with and below the tolerated limits. Conclusion: We can therefore conclude that the Abbott Alinity ci system meets the requirements set by scientific societies for the determination of haptoglobin.","PeriodicalId":12808,"journal":{"name":"GSC Biological and Pharmaceutical Sciences","volume":"9 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139591593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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