{"title":"Sreening of synthetic pharmaceutical drugs: PDE5 inhibitors, pain release, slimming groups in health supplements with LC-MS/MS","authors":"Linh Nguyen Trong, Nghia Cao Huu, Thi Chau Vinh","doi":"10.47866/2615-9252/vjfc.3965","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3965","url":null,"abstract":"Nowadays, there were many reports from Viet nam & international laboratories have detected many kinds of undeclared synthesis drugs, even of banned-chemicals in health supplement products (HSP). We developed methods on LC-MS/MS to detect synthesis pharmaceutical drugs in following groups of HSP: strengthen sexual for man group: sildenafil, Tadalafil, Vardenafil; Anti- arthritis group: Dexamethasone, Prednisolon, Betamethasone, Piroxicam; Slimming group: Sibutramin, Orlistate, Phenophtalein, Furosemid. We carried out method validation of specificities & limit of dectections of above drugs on mass spectrometer system of Triple Quad SCiex 55000, analysis 80 samples of each group HSP were collected from 4 provinces: Dong nai, Binh Duong, Ba Ria- Vung Tau, Binh Phuoc in 2020. We validated fragment transition & LOD of each drugs: Sidenafil (mz 475.2 → 100.2/ 283.2), LOD: 0.015ppm;Tadanafil (mz 390.1 → 268.1/262.1) LOD: 0.015 ppm; Vadernafil (mz 483.9 → 312.3/299.2), LOD: 0.07ppm; Prednisolon (mz 361 → 147/343.3), LOD: 0.075ppm; Dexamethason (mz 393.2 → 373.1/293.1); LOD: 0.02 ppm; Betamethason (mz 393.1 → 147.1/355.2)LOD: 0.075 ppm; Piroxicam (mz 330 → 266.2/146.1); LOD: 0.015 ppm; Sibutramin (mz 280.9 → 139/125.2), LOD: 0.75 ppm; Orlistate (mz 496. → 179,2/ 114.1), LOD: 1 ppm; Phenophtalein (mz 319,2 → 141.1/283.2), LOD: 0.01 ppm; Furosemid (mz 329.2 → 285/204), LOD: 0.01 ppm. Screenning results: Sidenafil nondetected/80 samples; Tadalafil positive 10/80 samples, Vadernafil nondetected/80 samples in man sexual enhancement HSP; Prednisonlon nondetected /80 samples, Dexamethason nondetected /80 samples; Betamethason positive 1/80 samples, Piroxicam nondetected /80 samples in anti-arthritis HSP. Sibutramin & Phenolphtalein positive 7/80 samples, Orlistate nondetected/80 samples, Furosemid nondetected/80 samples in slimming HSP.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83246572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-24DOI: 10.47866/2615-9252/vjfc.3964
Thanh Vuong Tong Thi, Cuong Truong Manh, Truong Nguyen Xuan, Thuy Linh Nguyen Thi, Ngoc Lan Dang Thi, Thanh Ha Pham Thi, Ha Thai Tran Nguyen, Binh Vu Ngan
Ketamin is a pharmaceutical compound that is abused as a narcotic drug, causing health problems for users. In controlling ketamine abuse, the detection of ketamine precursors can be a useful tool for tracing illegal ketamine production sites. In this study, an analytical method for determination of two ketamine precursors, 1-[(2-chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid (CCM) and 2-Hydroxy-2- (o-chloro phenyl) cyclohexanon (HCH), in wastewater using solid phase extraction (SPE) and liquid chromatogrphy tandem mass spectrometry (LC-MS/MS) was developed and validated. An LC-MS/MS system employing a Zorbax C18 column (2.1 x 100 mm, 1.8 µm) and a mobile phase composing acetonitrile and water containing 0.1% (m/v) of formic acid in a gradient program at the flow rate of 0.3 mL/min and injection volume of 5 µL. The detection of analytes was done with electrospray ionization at positive mode (ESI+) employing multi reaction monitoring (MRM) at m/z of 238.5 and 225.5 for for CCM and HCH, respectively. The method was validated according to requirements of AOAC International and EC and was proved as reliable for intended use.
{"title":"Development of analytical method for simultaneous detection of 1-[(2- chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid (CCM) and 2- Hydroxy-2- (o-chloro phenyl) cyclohexanon (HCH), two precursors of ketamine, in wastewater by LC-MS/MS","authors":"Thanh Vuong Tong Thi, Cuong Truong Manh, Truong Nguyen Xuan, Thuy Linh Nguyen Thi, Ngoc Lan Dang Thi, Thanh Ha Pham Thi, Ha Thai Tran Nguyen, Binh Vu Ngan","doi":"10.47866/2615-9252/vjfc.3964","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3964","url":null,"abstract":"Ketamin is a pharmaceutical compound that is abused as a narcotic drug, causing health problems for users. In controlling ketamine abuse, the detection of ketamine precursors can be a useful tool for tracing illegal ketamine production sites. In this study, an analytical method for determination of two ketamine precursors, 1-[(2-chlorophenyl) methyllimino methyl] cyclopentanol hydroclorid (CCM) and 2-Hydroxy-2- (o-chloro phenyl) cyclohexanon (HCH), in wastewater using solid phase extraction (SPE) and liquid chromatogrphy tandem mass spectrometry (LC-MS/MS) was developed and validated. An LC-MS/MS system employing a Zorbax C18 column (2.1 x 100 mm, 1.8 µm) and a mobile phase composing acetonitrile and water containing 0.1% (m/v) of formic acid in a gradient program at the flow rate of 0.3 mL/min and injection volume of 5 µL. The detection of analytes was done with electrospray ionization at positive mode (ESI+) employing multi reaction monitoring (MRM) at m/z of 238.5 and 225.5 for for CCM and HCH, respectively. The method was validated according to requirements of AOAC International and EC and was proved as reliable for intended use. ","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78860512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-24DOI: 10.47866/2615-9252/vjfc.3963
Dong Nguyen Kim, Trang Pham Huyen, Bac Nguyen Van, Thao Ha Phuong, Kien Hao Vo Thi, Tuong Vi Le Nguyen
The content of cadmium (Cd), lead (Pb) was determined in butterfly pea flower samples by atomic absorption Spectrophotometer (AAS). Method validation was evaluated through of parameters such as linear range, the limit of detection (LOD), the limit of quantitation (LOQ), recovery, repeatability, and intermediate precision. The validation results showed that the LOD of Cd, Pb were 1.20 and 6.20 μg/kg, respectively, while the LOQ of Cd and Pb were 4.01 and 20.5 μg/kg, respectively. The recovery of Cd is 96 - 98% and Pb is 98 - 100%. The relative repeat standard deviation (RSDr) of Cd, Pb is in the range of 1.50 - 2.50% and 1.90 - 2.13%, respectively. Meanwhile, the intermediate precision of Cd, Pb is 2.02% and 1.97%, respectively. The working range of the method is 0.2 - 4 μg/L (Cd) and 3 - 30 μg/L (Pb) with the standard curve equations having the correlation coefficient R2 ≥ 0.995. The quantitative method of Cd, Pb by atomic absorption Spectrophotometer is accurate and highly reliable, used to analyze butterfly pea flower samples collected in Cai Rang district, Can Tho city.
{"title":"Validation of methods for determination of lead and cadmium content in butterfly pea flowers (Clitoria ternatea) in the Cai Rang district, Can Tho city by atomic absorption Spectrophotometer AAS","authors":"Dong Nguyen Kim, Trang Pham Huyen, Bac Nguyen Van, Thao Ha Phuong, Kien Hao Vo Thi, Tuong Vi Le Nguyen","doi":"10.47866/2615-9252/vjfc.3963","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3963","url":null,"abstract":"The content of cadmium (Cd), lead (Pb) was determined in butterfly pea flower samples by atomic absorption Spectrophotometer (AAS). Method validation was evaluated through of parameters such as linear range, the limit of detection (LOD), the limit of quantitation (LOQ), recovery, repeatability, and intermediate precision. The validation results showed that the LOD of Cd, Pb were 1.20 and 6.20 μg/kg, respectively, while the LOQ of Cd and Pb were 4.01 and 20.5 μg/kg, respectively. The recovery of Cd is 96 - 98% and Pb is 98 - 100%. The relative repeat standard deviation (RSDr) of Cd, Pb is in the range of 1.50 - 2.50% and 1.90 - 2.13%, respectively. Meanwhile, the intermediate precision of Cd, Pb is 2.02% and 1.97%, respectively. The working range of the method is 0.2 - 4 μg/L (Cd) and 3 - 30 μg/L (Pb) with the standard curve equations having the correlation coefficient R2 ≥ 0.995. The quantitative method of Cd, Pb by atomic absorption Spectrophotometer is accurate and highly reliable, used to analyze butterfly pea flower samples collected in Cai Rang district, Can Tho city. ","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81973947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-23DOI: 10.47866/2615-9252/vjfc.3967
Que Mai Nguyen Thi, Hoa Do Thi, Thu Phuong Ngo Thi, Cam Pham Nguyet
A method using high performance liquid chromatography with UV - Vis detector was developed for quantification of taxifolin in supplements. In this study, the supplements were homogenized, extracted by ultrasonication with methanol. Taxifolin in test solution was separated on C18 column (250 × 4.6 mm, 5 µm) with mobile phase containing methanol and 0.3 % acetic acid solution (40 : 60) using isocratic elution at a flow rate of 1.0 mL/min, with UV detection at 290 nm. The analytical method was validated for specificity, linearity, precision and accuracy according to AOAC. The detector response for taxifolin was linear over the selected concentration range from 1.04 to 78.23 µg/mL with a correlation coefficient 0.9996. The repeatability (RSD, n = 7) were from 1.1 to 1.4 % respectively. The recovery was between 98.1 and 105.9 %. The method was successfully applied for analysis of taxifolin in 7 supplements at solid, liquid and oil form.
建立了一种高效液相色谱-紫外-可见检测器定量食品添加剂中紫杉醇的方法。在本研究中，补品均质，超声提取与甲醇。采用C18色谱柱(250 × 4.6 mm, 5µm)，流动相为甲醇和0.3%醋酸溶液(40∶60)，流速为1.0 mL/min，紫外检测波长为290 nm，等密度洗脱。根据AOAC对分析方法的特异性、线性度、精密度和准确度进行了验证。在1.04 ~ 78.23µg/mL范围内，检测器对紫杉醇的响应呈线性关系，相关系数为0.9996。重复性(RSD, n = 7)分别为1.1% ~ 1.4%。回收率在98.1% ~ 105.9%之间。该方法成功地应用于7种补充物中杉木素的固体、液体和油态分析。
{"title":"Determination of taxifolin in functional food samples by high - performance liquid chromatography (HPLC - UV Vis)","authors":"Que Mai Nguyen Thi, Hoa Do Thi, Thu Phuong Ngo Thi, Cam Pham Nguyet","doi":"10.47866/2615-9252/vjfc.3967","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3967","url":null,"abstract":"A method using high performance liquid chromatography with UV - Vis detector was developed for quantification of taxifolin in supplements. In this study, the supplements were homogenized, extracted by ultrasonication with methanol. Taxifolin in test solution was separated on C18 column (250 × 4.6 mm, 5 µm) with mobile phase containing methanol and 0.3 % acetic acid solution (40 : 60) using isocratic elution at a flow rate of 1.0 mL/min, with UV detection at 290 nm. The analytical method was validated for specificity, linearity, precision and accuracy according to AOAC. The detector response for taxifolin was linear over the selected concentration range from 1.04 to 78.23 µg/mL with a correlation coefficient 0.9996. The repeatability (RSD, n = 7) were from 1.1 to 1.4 % respectively. The recovery was between 98.1 and 105.9 %. The method was successfully applied for analysis of taxifolin in 7 supplements at solid, liquid and oil form.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"40 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86543674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-23DOI: 10.47866/2615-9252/vjfc.4008
Chien Dinh Viet, Hong Ngoc Nguyen Thi, Tien Bui Cao, Son Tran Cao, Thanh Tran Trung, Thanh Nguyen Ha, Ly Phung Cong, Thanh Ha Pham Thi, Anh Huong Nguyen Thi, Hong Hao Le Thi, Thu Thai Nguyen Hung
A determination method of ethylene oxide (ETO) and 2-chloro-ethanol (2-CE) in foods, especially those with high-fat content, by gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed. The analysis was performed with a TG-WAX column (60 m × 0.32 mm × 1 µm), with a programmable temperature vaporization-large volume injection method (PTV-LVI). ETO, 2-CE, and isotope internal standard 2-chloroethanol-d4 (2-CE-d4) were extracted by the QuOil (a variation of QuEChERS method), in combination with the use of stainless-steel balls, especially effective with high-fat matrices and analyzed by gas chromatography-mass spectrometry (GC-MS/MS). For both compounds, the method has high specificity and selectivity with a detection limit of 0.003 mg/kg, the linear range of 0.01 - 0.2 mg/kg, reproducibility and recovery meet AOAC requirements. This method has been applied to analyze 1668 samples during the period 2021-2022 at the National Institute for Food Control (NIFC).
建立了气相色谱-串联质谱法(GC-MS/MS)测定食品中环氧乙烷(ETO)和2-氯乙醇(2-CE)含量的方法。采用TG-WAX色谱柱(60 m × 0.32 mm × 1µm)，可编程温度蒸发-大体积进样法(PTV-LVI)进行分析。采用QuOil (QuEChERS方法的一种变体)结合不锈钢球提取ETO, 2-CE和同位素内标2-氯乙醇-d4 (2-CE-d4)，对高脂肪基质特别有效，并采用气相色谱-质谱(GC-MS/MS)分析。该方法对两种化合物均具有较高的特异性和选择性，检出限为0.003 mg/kg，线性范围为0.01 ~ 0.2 mg/kg，重现性和回收率均满足AOAC要求。该方法已应用于国家食品控制研究所(NIFC) 2021-2022年期间的1668份样品分析。
{"title":"Determination of ethylene oxide and 2-chloro-ethanol using gas chromatography-tandem mass spectrometry: Internal standard and stainless-steel ball coupled with QuOil in high-fat food extraction","authors":"Chien Dinh Viet, Hong Ngoc Nguyen Thi, Tien Bui Cao, Son Tran Cao, Thanh Tran Trung, Thanh Nguyen Ha, Ly Phung Cong, Thanh Ha Pham Thi, Anh Huong Nguyen Thi, Hong Hao Le Thi, Thu Thai Nguyen Hung","doi":"10.47866/2615-9252/vjfc.4008","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4008","url":null,"abstract":"A determination method of ethylene oxide (ETO) and 2-chloro-ethanol (2-CE) in foods, especially those with high-fat content, by gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed. The analysis was performed with a TG-WAX column (60 m × 0.32 mm × 1 µm), with a programmable temperature vaporization-large volume injection method (PTV-LVI). ETO, 2-CE, and isotope internal standard 2-chloroethanol-d4 (2-CE-d4) were extracted by the QuOil (a variation of QuEChERS method), in combination with the use of stainless-steel balls, especially effective with high-fat matrices and analyzed by gas chromatography-mass spectrometry (GC-MS/MS). For both compounds, the method has high specificity and selectivity with a detection limit of 0.003 mg/kg, the linear range of 0.01 - 0.2 mg/kg, reproducibility and recovery meet AOAC requirements. This method has been applied to analyze 1668 samples during the period 2021-2022 at the National Institute for Food Control (NIFC).","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87472390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.47866/2615-9252/vjfc.3953
Phuc Nguyen Do, Ngan Nguyen Ly Hoang, Hien Le Thi, Phong Ngo Thanh, Tram Tran Bao, Hien Nguyen Ngoc Thanh, Thanh Truc Nguyen Thi, Phuong Hoang Hoai
An alternative PCR method for rapid detection of Campylobacter spp. in milk powder was validated according to TCVN 12365-2:2018 (ISO 16140-2:2016). In this study, performed with paired study, validation examines the following parameters: (1) sensitivity (sensitivity for the alternative method: SEalt, sensitivity for the reference method: SEref, relative trueness: RT, false positive ratio for the alternative method: FPR), (2) relative level of detection (RLOD) and (3) inclusivity and exclusivity. The result showed that: sensitivity for the alternative method: 85.7%, sensitivity for the reference method 92.8%, relative trueness: 85%, false positive ratio for the alternative method: 16.7%, relative level of detection: 0,859 CFU/25g. For inclusitivity testing, two reference strains were detected. For exclusitivity testing, all of 20 strains were not detected. All the parameters tested were met according to TCVN 12365-2:2018 (ISO 16140-2: 2016) requirements.
根据TCVN 12365-2:2018 (ISO 16140-2:2016)的要求，建立了一种快速检测奶粉中弯曲杆菌的PCR方法。在本研究中，采用配对研究，验证检验了以下参数:(1)灵敏度(替代方法的灵敏度:seal，参考方法的灵敏度:SEref，相对真实度:RT，替代方法的假阳性率:FPR)，(2)相对检测水平(RLOD)和(3)包容性和排他性。结果表明:替代方法的灵敏度为85.7%，参考方法的灵敏度为92.8%，相对正确率为85%，替代方法的假阳性率为16.7%，相对检出水平为0.859 CFU/25g。在包容性测试中，检测到两个参考菌株。20株菌株均未检出。所有测试参数均符合TCVN 12365-2:2018 (ISO 16140- 2:20 16)要求。
{"title":"Validation of a PCR method for the detection Campylobacter spp. in milk powder","authors":"Phuc Nguyen Do, Ngan Nguyen Ly Hoang, Hien Le Thi, Phong Ngo Thanh, Tram Tran Bao, Hien Nguyen Ngoc Thanh, Thanh Truc Nguyen Thi, Phuong Hoang Hoai","doi":"10.47866/2615-9252/vjfc.3953","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3953","url":null,"abstract":"An alternative PCR method for rapid detection of Campylobacter spp. in milk powder was validated according to TCVN 12365-2:2018 (ISO 16140-2:2016). In this study, performed with paired study, validation examines the following parameters: (1) sensitivity (sensitivity for the alternative method: SEalt, sensitivity for the reference method: SEref, relative trueness: RT, false positive ratio for the alternative method: FPR), (2) relative level of detection (RLOD) and (3) inclusivity and exclusivity. The result showed that: sensitivity for the alternative method: 85.7%, sensitivity for the reference method 92.8%, relative trueness: 85%, false positive ratio for the alternative method: 16.7%, relative level of detection: 0,859 CFU/25g. For inclusitivity testing, two reference strains were detected. For exclusitivity testing, all of 20 strains were not detected. All the parameters tested were met according to TCVN 12365-2:2018 (ISO 16140-2: 2016) requirements.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84132710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-21DOI: 10.47866/2615-9252/vjfc.3951
Huong Dang Thi, Ba Tran Hong, L. Le Thanh, C. Nguyen Van, Hanh Ninh Thi, T. Nguyen Thanh, Hong Hao Le Thi, Xuan Nguyen Thi
Bacillus subtilis is a widely used bacterial strain in probiotic production. The strains belong to this species are endosporogenic, tolerant to acidic pH conditions in the stomach, and many have been shown to produce enzymes that aid in food digestion and inhibit pathogenic microorganisms. In this study, a detection and qualitification method was developed based on amplification of aprE gene ultilising realtime PCR. The aprE target gene encodes subtilisin toxin precursor and is a highly conservative and specific gene of B. subtilis. The method yielded high sensitivity with LOD is 102 (CFU/mL), LOQ is 102 (CFU/mL), specificity, and accuracy are both 100%. The preparatory tools were constructed using cycle threshold (Ct) compare with B. subtilis concentration, which showed linear correlation (R2 = 1, slope = - 3.584).
{"title":"New realtime PCR method development for detection and quantification of Bacillus subtilis","authors":"Huong Dang Thi, Ba Tran Hong, L. Le Thanh, C. Nguyen Van, Hanh Ninh Thi, T. Nguyen Thanh, Hong Hao Le Thi, Xuan Nguyen Thi","doi":"10.47866/2615-9252/vjfc.3951","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3951","url":null,"abstract":"Bacillus subtilis is a widely used bacterial strain in probiotic production. The strains belong to this species are endosporogenic, tolerant to acidic pH conditions in the stomach, and many have been shown to produce enzymes that aid in food digestion and inhibit pathogenic microorganisms. In this study, a detection and qualitification method was developed based on amplification of aprE gene ultilising realtime PCR. The aprE target gene encodes subtilisin toxin precursor and is a highly conservative and specific gene of B. subtilis. The method yielded high sensitivity with LOD is 102 (CFU/mL), LOQ is 102 (CFU/mL), specificity, and accuracy are both 100%. The preparatory tools were constructed using cycle threshold (Ct) compare with B. subtilis concentration, which showed linear correlation (R2 = 1, slope = - 3.584).","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74209398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-20DOI: 10.47866/2615-9252/vjfc.4004
Son Tran Hung
Nutraceutical is one of the new concepts appearing in recent years, referring to food products derived from natural sources that benefit human health in preventing and treating diseases, besides providing nutritional value. During the development of these products, it is important to evaluate the toxicity and bioactivity of potential compounds and to study the mechanism of action of these nutraceutical compounds at the cellular and molecular levels. Among the many different experimental models, the in vitro cell-based model has emerged as a model with many advantages for preliminary screening of nutraceuticals activity and toxicity before conducting further studies on in vivo models. This review summarizes some basic techniques commonly used to screen nutraceuticals' toxicity. Recent studies of the bioactivity of nutraceuticals in various areas, such as antioxidant, anti-inflammation, antiobesity, neuroprotection, and gut health improvement, are also reviewed to introduce the application of the cell-based model in nutraceutical bioactivity research. New modern techniques using in vitro cell-based models have been applied in this field, such as highthroughput screening, 3D-cell culture, and organ-on-a-chip are also discussed in this paper.
{"title":"Bioactivity and toxicity evaluation of nutraceuticals using in vitro cell-based models: A review","authors":"Son Tran Hung","doi":"10.47866/2615-9252/vjfc.4004","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4004","url":null,"abstract":"Nutraceutical is one of the new concepts appearing in recent years, referring to food products derived from natural sources that benefit human health in preventing and treating diseases, besides providing nutritional value. During the development of these products, it is important to evaluate the toxicity and bioactivity of potential compounds and to study the mechanism of action of these nutraceutical compounds at the cellular and molecular levels. Among the many different experimental models, the in vitro cell-based model has emerged as a model with many advantages for preliminary screening of nutraceuticals activity and toxicity before conducting further studies on in vivo models. This review summarizes some basic techniques commonly used to screen nutraceuticals' toxicity. Recent studies of the bioactivity of nutraceuticals in various areas, such as antioxidant, anti-inflammation, antiobesity, neuroprotection, and gut health improvement, are also reviewed to introduce the application of the cell-based model in nutraceutical bioactivity research. New modern techniques using in vitro cell-based models have been applied in this field, such as highthroughput screening, 3D-cell culture, and organ-on-a-chip are also discussed in this paper.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79307120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-20DOI: 10.47866/2615-9252/vjfc.3955
Ngoc Mai Dinh Thi, Van Nguyen Ha, Phong Tran Huu, T. Nguyen Thanh, Nuong Nguyen Kim, Minh Nguyen Hong
Kombucha beverage is a traditional and popular natural fermented beverage consumed across the globe. Kombucha beverage is produced by fermenting sweetened black tea, sucrose with consortium of acetic acid bacteria and yeasts. It is important to understand the microbial composition in Kombucha to facilitate better control of the fermentation process. Therefore, this study characterized the dominant acetic acid bacteria in Kombucha sample. Acetic acid bacteria isolated from the Kombucha using glucose-ethanol medium. Based on morphological, biochemical characterization, and 16S rRNA gene, 14 isolated strains belong to 2 species and were identified as Komagataeibacter saccharivorans and Komagataeibacter rhaeticus. These strains showed good biofilm forming abilities. Furthermore, the antimicrobial activity against five pathogenic bacteria including Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Samonella enterica ATCC 13076, Pseudomonas aeruginosa ATCC 27853, Bacillus cereus ATCC 14579 of isolated acetic acid bacteria strains was also determined.
{"title":"Isolation and characterization of dominant acetic acid bacteria isolated from Kombucha tea","authors":"Ngoc Mai Dinh Thi, Van Nguyen Ha, Phong Tran Huu, T. Nguyen Thanh, Nuong Nguyen Kim, Minh Nguyen Hong","doi":"10.47866/2615-9252/vjfc.3955","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3955","url":null,"abstract":"Kombucha beverage is a traditional and popular natural fermented beverage consumed across the globe. Kombucha beverage is produced by fermenting sweetened black tea, sucrose with consortium of acetic acid bacteria and yeasts. It is important to understand the microbial composition in Kombucha to facilitate better control of the fermentation process. Therefore, this study characterized the dominant acetic acid bacteria in Kombucha sample. Acetic acid bacteria isolated from the Kombucha using glucose-ethanol medium. Based on morphological, biochemical characterization, and 16S rRNA gene, 14 isolated strains belong to 2 species and were identified as Komagataeibacter saccharivorans and Komagataeibacter rhaeticus. These strains showed good biofilm forming abilities. Furthermore, the antimicrobial activity against five pathogenic bacteria including Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Samonella enterica ATCC 13076, Pseudomonas aeruginosa ATCC 27853, Bacillus cereus ATCC 14579 of isolated acetic acid bacteria strains was also determined. ","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84452415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-19DOI: 10.47866/2615-9252/vjfc.3958
Dat Nguyen Thanh, Hong Hanh Nguyen Thi, Thu Dam Thi, Hieu Nguyen Van, Phuong Lai Thi, Bac Duong The
High performance liquid chromatography with diode-array detector (HPLC-DAD) has been fully validated according to AOAC requirements for the determination of adenosine and cordycepin in dietary supplements containing Cordyceps. Chromatographic conditions using InertSustain C18 (250 mm × 4.6 mm; 5 µm) column, with gradient program composed of methanol and water eluted in 35 min, diode-array detector at 260 nm, flow rate of 1.0 mL/min. The study has determined the content of adenosine and cordycepin in 24 dietary supplement samples containing Cordyceps in different dosage forms circulating on the Hanoi market. The results showed that the contents of the two substances studied in the samples fluctuated greatly (adenosine from 28.19 - 956.3 µg/g with solid and oily form, from 1.26 - 25.31 µg/mL with the liquid form; cordycepin from 5.25 - 1287 µg/g with solid and oily form, from 1.91 - 32.38 µg/mL with liquid form), especially one sample was detected without both adenosine and cordycepin, one sample did not have adenosine.
{"title":"Determination of adenosine and cordycepin in dietary supplements circulating on the Hanoi market","authors":"Dat Nguyen Thanh, Hong Hanh Nguyen Thi, Thu Dam Thi, Hieu Nguyen Van, Phuong Lai Thi, Bac Duong The","doi":"10.47866/2615-9252/vjfc.3958","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.3958","url":null,"abstract":"High performance liquid chromatography with diode-array detector (HPLC-DAD) has been fully validated according to AOAC requirements for the determination of adenosine and cordycepin in dietary supplements containing Cordyceps. Chromatographic conditions using InertSustain C18 (250 mm × 4.6 mm; 5 µm) column, with gradient program composed of methanol and water eluted in 35 min, diode-array detector at 260 nm, flow rate of 1.0 mL/min. The study has determined the content of adenosine and cordycepin in 24 dietary supplement samples containing Cordyceps in different dosage forms circulating on the Hanoi market. The results showed that the contents of the two substances studied in the samples fluctuated greatly (adenosine from 28.19 - 956.3 µg/g with solid and oily form, from 1.26 - 25.31 µg/mL with the liquid form; cordycepin from 5.25 - 1287 µg/g with solid and oily form, from 1.91 - 32.38 µg/mL with liquid form), especially one sample was detected without both adenosine and cordycepin, one sample did not have adenosine. ","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"385 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76652989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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