Pub Date : 2023-06-20DOI: 10.47866/2615-9252/vjfc.4074
Trang Luu Thi Huyen, Le Vu Thi Nhat, Trang Vu Thi, Hao Le Thi Hong
The study was carried out to develop a method for determining the biotin content in nutritional products and supplements by liquid chromatography-mass spectrometry (LC-MS/ MS) combined with a fast and simple pretreatment. The analyzed sample was hydrolyzed in acetate buffer (50 mM, pH 4) with the presence of ascorbate solution (10%) at 120 ± 1°C. After adding the internal standard (2H4-biotin), the mixture was analyzed on LC-MS/ MS system using C18 (100 mm × 2.1 mm; 1.7 µm) column and 0.1% formic acid and MeOH as mobile phases. The analyte was detected on an MS/MS system with an ESI (+) ionization source. The method was validated following the AOAC criteria. The method detection limit was 0.15 - 4.20 µg/100g, the method quantitative limit was in the range of 0.5 - 14.0 µg/100g, the recovery range was 81.3 - 107.3%, the repeatability was in the range of 2.00 - 7.30 %, the reproducibility was 2.50 - 7.70%, which were in accordance with AOAC requirements. The method has been applied to analyze biotin content in 10 nutritional products and 10 supplements purchased in the market.
建立了一种快速、简便的前处理相结合的液相色谱-质谱联用法测定营养品和保健品中生物素含量的方法。所分析的样品在醋酸缓冲液(50 mM, pH 4)中水解，抗坏血酸溶液(10%)存在，温度为120±1℃。加入内标物(2h4 -生物素)后，采用C18 (100 mm × 2.1 mm;1.7µm)色谱柱，流动相为0.1%甲酸和甲醇。分析物在ESI(+)电离源的MS/MS系统上检测。按照AOAC标准对方法进行了验证。方法检出限为0.15 ~ 4.20µg/100g，定量限为0.5 ~ 14.0µg/100g，加样回收率为81.3 ~ 107.3%，重复性为2.00 ~ 7.30%，重现性为2.50 ~ 7.70%，符合AOAC要求。该方法已应用于市场上购买的10种营养品和10种补品的生物素含量分析。
{"title":"Development of liquid chromatography-mass spectrometry method to determine biotin content in nutritional products and supplements","authors":"Trang Luu Thi Huyen, Le Vu Thi Nhat, Trang Vu Thi, Hao Le Thi Hong","doi":"10.47866/2615-9252/vjfc.4074","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4074","url":null,"abstract":"The study was carried out to develop a method for determining the biotin content in nutritional products and supplements by liquid chromatography-mass spectrometry (LC-MS/ MS) combined with a fast and simple pretreatment. The analyzed sample was hydrolyzed in acetate buffer (50 mM, pH 4) with the presence of ascorbate solution (10%) at 120 ± 1°C. After adding the internal standard (2H4-biotin), the mixture was analyzed on LC-MS/ MS system using C18 (100 mm × 2.1 mm; 1.7 µm) column and 0.1% formic acid and MeOH as mobile phases. The analyte was detected on an MS/MS system with an ESI (+) ionization source. The method was validated following the AOAC criteria. The method detection limit was 0.15 - 4.20 µg/100g, the method quantitative limit was in the range of 0.5 - 14.0 µg/100g, the recovery range was 81.3 - 107.3%, the repeatability was in the range of 2.00 - 7.30 %, the reproducibility was 2.50 - 7.70%, which were in accordance with AOAC requirements. The method has been applied to analyze biotin content in 10 nutritional products and 10 supplements purchased in the market.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81619633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-16DOI: 10.47866/2615-9252/vjfc.4075
Tam Phan Thanh, Phuong Luu Thi Ngoc, Hang Vu Thi Minh
In the world, almost all chicken meat is used in chilled or frozen meat to ensure quality and food hygiene and safety, but in Viet Nam, the habit of using hot chicken meat is still quite common (do not lower the carcass temperature after slaughter, in storing, transporting or consuming), leading to a very high risk of food poisoning from this raw material. The study was conducted to evaluate the quality of cool (chilled) chicken meat of 02 common breeds of chicken raised in Viet Nam, Indian River Meat (coding C2) and Tam Hoang (coding C3), raised at commercial chicken farms, slaughtered and chilled at the factory of 3F Food Co., Ltd (Binh Duong province). Chicken carcasses were chilled down to 0-4°C immediately after slaughter and then cut and packed for transportation at this temperature. This study has developed 19/75 sensory property terms that strongly influence the overall quality of cool chicken meat. The results of evaluating the quality of cool chicken meat at 1, 3, 5, 7, 10, and 14 days showed that, in the period from day 1 to day 5, the values of pH, TVB-N, and total aerobic microorganisms (TAM) values of chicken breasts and thighs of both C2 and C3 showed very little variation and were within the allowable limits, respectively. The sensory properties were evaluated not to be inferior to that of freshly slaughtered meat surgery. By day 7, there were signs of quality deterioration, pH, TVB-N, and TAM all exceeded the regulations, and the level of bad sensory properties gradually increased. Thus, chicken meat products in this study should be stored for up to 5 days.
{"title":"Research on quality and shelf life of cool meat of some popular chicken breeds in Viet Nam","authors":"Tam Phan Thanh, Phuong Luu Thi Ngoc, Hang Vu Thi Minh","doi":"10.47866/2615-9252/vjfc.4075","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4075","url":null,"abstract":"In the world, almost all chicken meat is used in chilled or frozen meat to ensure quality and food hygiene and safety, but in Viet Nam, the habit of using hot chicken meat is still quite common (do not lower the carcass temperature after slaughter, in storing, transporting or consuming), leading to a very high risk of food poisoning from this raw material. The study was conducted to evaluate the quality of cool (chilled) chicken meat of 02 common breeds of chicken raised in Viet Nam, Indian River Meat (coding C2) and Tam Hoang (coding C3), raised at commercial chicken farms, slaughtered and chilled at the factory of 3F Food Co., Ltd (Binh Duong province). Chicken carcasses were chilled down to 0-4°C immediately after slaughter and then cut and packed for transportation at this temperature. This study has developed 19/75 sensory property terms that strongly influence the overall quality of cool chicken meat. The results of evaluating the quality of cool chicken meat at 1, 3, 5, 7, 10, and 14 days showed that, in the period from day 1 to day 5, the values of pH, TVB-N, and total aerobic microorganisms (TAM) values of chicken breasts and thighs of both C2 and C3 showed very little variation and were within the allowable limits, respectively. The sensory properties were evaluated not to be inferior to that of freshly slaughtered meat surgery. By day 7, there were signs of quality deterioration, pH, TVB-N, and TAM all exceeded the regulations, and the level of bad sensory properties gradually increased. Thus, chicken meat products in this study should be stored for up to 5 days.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90703137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.47866/2615-9252/vjfc.4077
Loan Huynh Thi Phuong, Thu Do Thi Anh, Tu Nguyen Thi Thanh, Ngoc Le Nguyen Bao, Tran Nguyen Que
The study aims to evaluate the degree of fat oxidation and the reuse of fried oils in fried meat and fish ball products sold in Ninh Kieu District, Can Tho City. The fat content was determined in fried products, as follows: fish balls (15 - 21%), beef balls (11 -14%), and shrimp balls (15 - 23%). Because of the difference in the fat origin of the three types of fried products, the oxidation levels of these products are also different; therefore, this is possible through parameters related to the variation of fat such as acid index, peroxide index, malondialdehyde (MDA) content. The concentration of MDA was the highest in fried fish balls, and the lowest value was in beef balls. After surveying for five consecutive days, the highest concentration of MDA in fried products was obtained in the last days of the cycle (4th day). The acid value in the fried oil samples ranges from 0 - 1 mg, the peroxide index from 2 - 5.78 meq/kg, and the concentration of MDA ranges from 0.22 - 2.98 µmol/L. A combination of results of the degree of fat oxidation in fried meat and fish ball products and frying oils indicates that the sellers reused the oils and tended to renew them every 3 - 4 days.
{"title":"Food safety assessment for fried meat and fish ball in Ninh Kieu district, Can Tho city","authors":"Loan Huynh Thi Phuong, Thu Do Thi Anh, Tu Nguyen Thi Thanh, Ngoc Le Nguyen Bao, Tran Nguyen Que","doi":"10.47866/2615-9252/vjfc.4077","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4077","url":null,"abstract":"The study aims to evaluate the degree of fat oxidation and the reuse of fried oils in fried meat and fish ball products sold in Ninh Kieu District, Can Tho City. The fat content was determined in fried products, as follows: fish balls (15 - 21%), beef balls (11 -14%), and shrimp balls (15 - 23%). Because of the difference in the fat origin of the three types of fried products, the oxidation levels of these products are also different; therefore, this is possible through parameters related to the variation of fat such as acid index, peroxide index, malondialdehyde (MDA) content. The concentration of MDA was the highest in fried fish balls, and the lowest value was in beef balls. After surveying for five consecutive days, the highest concentration of MDA in fried products was obtained in the last days of the cycle (4th day). The acid value in the fried oil samples ranges from 0 - 1 mg, the peroxide index from 2 - 5.78 meq/kg, and the concentration of MDA ranges from 0.22 - 2.98 µmol/L. A combination of results of the degree of fat oxidation in fried meat and fish ball products and frying oils indicates that the sellers reused the oils and tended to renew them every 3 - 4 days.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"1982 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86538017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-31DOI: 10.47866/2615-9252/vjfc.4076
Hanh Tran Thuy, Hoa Mac Thi Thanh, Khanh Cao Cong, Hung Nguyen Quang, Son Tran Cao, An Tran Thi Hoai, Binh Vu Ngan, Lan Dang Thi Ngoc, Anh Nguyen Thi Kieu
A sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous analysis of 4 compounds used for treating hypertension as adulterants in herbal products. The method was validated in terms of selectivity, linearity, accuracy, limit of detection (LOD), limit of quantitation (LOQ), and precision in accordance with AOAC and ICH guidelines. The LOD and LOQ of all compounds were about 0.3 ng/mL and 1 ng/mL, respectively. The linearity was good (R2 > 0.999), with intra-day and inter-day precision levels not more than 9.85% and 8.51%, respectively, and 81.9 – 109.8% accuracies. Thirty commercial herbal products consisting of traditional medicines and dietary supplements available in Vietnamese markets were tested. While none contained detectable amounts of the 4 antihypertensive compounds, the developed LC-MS/MS procedure can be used for routine analysis to monitor illegal adulteration in various forms of herbal products.
{"title":"Simultaneous analysis of 4 specific antihypertensive adulterants in herbal products using LC-MS/MS","authors":"Hanh Tran Thuy, Hoa Mac Thi Thanh, Khanh Cao Cong, Hung Nguyen Quang, Son Tran Cao, An Tran Thi Hoai, Binh Vu Ngan, Lan Dang Thi Ngoc, Anh Nguyen Thi Kieu","doi":"10.47866/2615-9252/vjfc.4076","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4076","url":null,"abstract":"A sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous analysis of 4 compounds used for treating hypertension as adulterants in herbal products. The method was validated in terms of selectivity, linearity, accuracy, limit of detection (LOD), limit of quantitation (LOQ), and precision in accordance with AOAC and ICH guidelines. The LOD and LOQ of all compounds were about 0.3 ng/mL and 1 ng/mL, respectively. The linearity was good (R2 > 0.999), with intra-day and inter-day precision levels not more than 9.85% and 8.51%, respectively, and 81.9 – 109.8% accuracies. Thirty commercial herbal products consisting of traditional medicines and dietary supplements available in Vietnamese markets were tested. While none contained detectable amounts of the 4 antihypertensive compounds, the developed LC-MS/MS procedure can be used for routine analysis to monitor illegal adulteration in various forms of herbal products.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78338137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-26DOI: 10.47866/2615-9252/vjfc.4078
Thanh Nguyen Duc, Tuan Vy Quoc, Vu Nguyen Dang Long, Dung Nguyen Quang, Toan Pham Van, Cuong Nguyen Cong, Anh Nguyen Thi Van
The Tinospora sinensis Merr. was ultrasonically extracted using 80% ethanol at a temperature of 50°C. The resulting solution was distilled to remove the solvent under reduced pressure, yielding the extract. The extract was then dissolved in distilled water, and this solution was administered to the experimental mice. The results showed that even at the maximum allowable dose, no mice experienced mortality. The anti-inflammatory effects of Tinospora sinensis Merr. extract were tested in experiments. The results demonstrated that at a dose equivalent to 1.6 g of plant material per kilogram of body weight per day, the extract exhibited acute anti-inflammatory activity in a model of carrageenan-induced foot edema in mice, comparable to a dose of 80 mg/kg body weight per day of aspirin. Additionally, at a dose equivalent to 2.8 g of plant material per kilogram of body weight per day, the extract showed chronic anti-inflammatory effects in a model of amian-induced granuloma in white mice. These findings suggest a novel direction in using plant-derived medicines, specifically the Tinospora sinensis Merr.
{"title":"Study on the anti-arthritic effects of Tinospora sinensis Merr.","authors":"Thanh Nguyen Duc, Tuan Vy Quoc, Vu Nguyen Dang Long, Dung Nguyen Quang, Toan Pham Van, Cuong Nguyen Cong, Anh Nguyen Thi Van","doi":"10.47866/2615-9252/vjfc.4078","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4078","url":null,"abstract":"The Tinospora sinensis Merr. was ultrasonically extracted using 80% ethanol at a temperature of 50°C. The resulting solution was distilled to remove the solvent under reduced pressure, yielding the extract. The extract was then dissolved in distilled water, and this solution was administered to the experimental mice. The results showed that even at the maximum allowable dose, no mice experienced mortality. The anti-inflammatory effects of Tinospora sinensis Merr. extract were tested in experiments. The results demonstrated that at a dose equivalent to 1.6 g of plant material per kilogram of body weight per day, the extract exhibited acute anti-inflammatory activity in a model of carrageenan-induced foot edema in mice, comparable to a dose of 80 mg/kg body weight per day of aspirin. Additionally, at a dose equivalent to 2.8 g of plant material per kilogram of body weight per day, the extract showed chronic anti-inflammatory effects in a model of amian-induced granuloma in white mice. These findings suggest a novel direction in using plant-derived medicines, specifically the Tinospora sinensis Merr.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89938872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-23DOI: 10.47866/2615-9252/vjfc.4072
Quynh Pham Thi Diem, Mai Pham Thi Ngoc, Anh Hoang Quoc, Thuy Le Minh, Huong Nguyen Thi, Hoa Vu Khanh, H. Do Thi Thu, Xuyen Nguyen Thi, Hung Nguyen Xuan, Binh Chu Dinh, Nam Vu Duc
Polychlorinated biphenyls (PCBs) are a typical group of persistent organic pollutants (POPs), which have been listed under Annex A (Elimination) and Annex C (Unintentional production) of the Stockholm Convention. In this study, a gas chromatography tandem mass spectrometry method was developed and applied to analyze concentrations of 28 PCB congeners in some Vietnamese marine fish samples. PCBs in fish samples were ultrasonically extracted with an acetone/n-hexane (1/1, v/v) mixture. The extracts were cleaned up by using multilayer silica gel columns with dichloromethane/n-hexane (1/1, v/v) as elution solvent. PCBs were separated on a DB-5MS column and quantified by using a triple quadrupole mass spectrometer. The MS detector was operated in positive electron impact ionization (EI) mode and selected reaction monitoring (SRM) mode. Calibration curves of 28 PCBs exhibited good linearity (R2 ≥ 0.9998). Instrument detection limits (IDLs) and method detection limits (MDLs) ranged from 0.08 to 0.023 ng/mL and from 0.07 to 1.84 ng/g, respectively. Recoveries of 28 PCBs native and 7 labeled standards in matrix-spike samples ranged from 62.3 to 88.1% and from 75.5 to 91.9%, satisfying criteria proposed by AOAC (recovery 60-115% for 10-100 ppb levels). The validated method was applied to analyze 10 marine fish samples, showing levels of ∑28PCBs from 17 to 851 (mean 230) ng/g lipid. The sum of 6in-PCBs (PCB-28; -52; -101; -138; -153; -180) based on wet weight (w/w) ranged from 1.24 to 3.15 ng/g, which is lower than the maximum level recommended by The European Union (75 ng/g (w/w). Congeners PCB-126 and PCB-169 were not detected in marine fish samples.
{"title":"Determination of polychlorinated biphenyls in marine fish samples by gas chromatography tandem mass spectrometry (GC-MS/MS)","authors":"Quynh Pham Thi Diem, Mai Pham Thi Ngoc, Anh Hoang Quoc, Thuy Le Minh, Huong Nguyen Thi, Hoa Vu Khanh, H. Do Thi Thu, Xuyen Nguyen Thi, Hung Nguyen Xuan, Binh Chu Dinh, Nam Vu Duc","doi":"10.47866/2615-9252/vjfc.4072","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4072","url":null,"abstract":"Polychlorinated biphenyls (PCBs) are a typical group of persistent organic pollutants (POPs), which have been listed under Annex A (Elimination) and Annex C (Unintentional production) of the Stockholm Convention. In this study, a gas chromatography tandem mass spectrometry method was developed and applied to analyze concentrations of 28 PCB congeners in some Vietnamese marine fish samples. PCBs in fish samples were ultrasonically extracted with an acetone/n-hexane (1/1, v/v) mixture. The extracts were cleaned up by using multilayer silica gel columns with dichloromethane/n-hexane (1/1, v/v) as elution solvent. PCBs were separated on a DB-5MS column and quantified by using a triple quadrupole mass spectrometer. The MS detector was operated in positive electron impact ionization (EI) mode and selected reaction monitoring (SRM) mode. Calibration curves of 28 PCBs exhibited good linearity (R2 ≥ 0.9998). Instrument detection limits (IDLs) and method detection limits (MDLs) ranged from 0.08 to 0.023 ng/mL and from 0.07 to 1.84 ng/g, respectively. Recoveries of 28 PCBs native and 7 labeled standards in matrix-spike samples ranged from 62.3 to 88.1% and from 75.5 to 91.9%, satisfying criteria proposed by AOAC (recovery 60-115% for 10-100 ppb levels). The validated method was applied to analyze 10 marine fish samples, showing levels of ∑28PCBs from 17 to 851 (mean 230) ng/g lipid. The sum of 6in-PCBs (PCB-28; -52; -101; -138; -153; -180) based on wet weight (w/w) ranged from 1.24 to 3.15 ng/g, which is lower than the maximum level recommended by The European Union (75 ng/g (w/w). Congeners PCB-126 and PCB-169 were not detected in marine fish samples.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89111986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.47866/2615-9252/vjfc.4073
Anh Nguyen Tuan, Trac Nguyen Duc Anh, Thien Nguyen Quang, Nhan Le Cong
Lipophilic marine biotoxins include Azaspiracid-1 (AZA-1), Azaspiracid-2 (AZA-2), Azaspiracid-3 (AZA-3), Pectenotoxin-2 (PTX 2), Okadaic acid (OA), Dinophysistoxin-2 (DTX-2), Dinophysistoxin-1 (DTX-1), Yessotoxin (YTX), and 1-Homoyessotoxin (Homo-YTX) were extracted with methanol, followed by cleaning up with solid phase extraction technique (SPE). Lipophilic toxins were confirmed and quantified by liquid chromatography coupled with triple quadrupole mass spectrometry (LC/MS/MS) using calibration curves on the solvent. The quantification limits of this method satisfied the requirements of the European Maximum Residue Limit (MRLs) with 25 µg/kg for the YTX group and 10 µg/kg for AZA, OA, and PTX groups. To validate the effectiveness of this method, matrices of clams, fish, and mixed seafood were collected and analyzed (recovery ranged from 92.4 - 101.5%, and relative standard deviation was less than 20%). The method was used successfully to participate in a proficiency testing program organized by Quasimeme (z-score in the range of ±2)
{"title":"Determination of lipophilic marine biotoxins in aquatic products by liquid chromatography coupled with triple quadrupole mass spectrometry","authors":"Anh Nguyen Tuan, Trac Nguyen Duc Anh, Thien Nguyen Quang, Nhan Le Cong","doi":"10.47866/2615-9252/vjfc.4073","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4073","url":null,"abstract":"Lipophilic marine biotoxins include Azaspiracid-1 (AZA-1), Azaspiracid-2 (AZA-2), Azaspiracid-3 (AZA-3), Pectenotoxin-2 (PTX 2), Okadaic acid (OA), Dinophysistoxin-2 (DTX-2), Dinophysistoxin-1 (DTX-1), Yessotoxin (YTX), and 1-Homoyessotoxin (Homo-YTX) were extracted with methanol, followed by cleaning up with solid phase extraction technique (SPE). Lipophilic toxins were confirmed and quantified by liquid chromatography coupled with triple quadrupole mass spectrometry (LC/MS/MS) using calibration curves on the solvent. The quantification limits of this method satisfied the requirements of the European Maximum Residue Limit (MRLs) with 25 µg/kg for the YTX group and 10 µg/kg for AZA, OA, and PTX groups. To validate the effectiveness of this method, matrices of clams, fish, and mixed seafood were collected and analyzed (recovery ranged from 92.4 - 101.5%, and relative standard deviation was less than 20%). The method was used successfully to participate in a proficiency testing program organized by Quasimeme (z-score in the range of ±2)","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85210348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-14DOI: 10.47866/2615-9252/vjfc.4070
Binh Nguyen Thi Ha, Hien Dang Thu, Son Tran Cao
Toxic phthalates that can be released from food packaging into food are currently a matter of concern, affecting consumers’ health. However, in Vietnam, no studies have been published on this group of substances in food packaging and films. In this research, a method for the simultaneous determination of 7 typical phthalates, including benzyl butyl phthalate (BBP), dibutyl phthalate (DBP), di(2-ethylhexyl) phthalate (DEHP), di-n-octyl phthalate (DNOP), diisodecyl phthalate (DIDP), diisononyl phthalate (DINP), di-(2-ethylhexyl) adipate (DEHA) in food wrap samples by gas chromatography mass spectrometry (GC-MS) using internal standards has been developed and validated. The limit of detection and limit of quantification for all substances was 0.03 mg/kg and 0.10 mg/kg, respectively. The recoveries of the method were in the range of 82-105%. Relative standard deviation (RSD) ranged from 5.6 to 7.5%. The method has been applied effectively to analyze the content of phthalates in 35 food wrap samples that were collected from markets in Hanoi. Analytical results showed that ten samples were detected with phthalates, of which three samples contained DEHA exceeding the specific migration limit (SML). This study contributes to consumer health protection and advises the regulatory agency on the addition of safety regulations for PVC food wrap in particular and other food packaging materials in general.
{"title":"Determination of phthalate in PVC food wrap by gas chromatography mass spectrometry","authors":"Binh Nguyen Thi Ha, Hien Dang Thu, Son Tran Cao","doi":"10.47866/2615-9252/vjfc.4070","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4070","url":null,"abstract":"Toxic phthalates that can be released from food packaging into food are currently a matter of concern, affecting consumers’ health. However, in Vietnam, no studies have been published on this group of substances in food packaging and films. In this research, a method for the simultaneous determination of 7 typical phthalates, including benzyl butyl phthalate (BBP), dibutyl phthalate (DBP), di(2-ethylhexyl) phthalate (DEHP), di-n-octyl phthalate (DNOP), diisodecyl phthalate (DIDP), diisononyl phthalate (DINP), di-(2-ethylhexyl) adipate (DEHA) in food wrap samples by gas chromatography mass spectrometry (GC-MS) using internal standards has been developed and validated. The limit of detection and limit of quantification for all substances was 0.03 mg/kg and 0.10 mg/kg, respectively. The recoveries of the method were in the range of 82-105%. Relative standard deviation (RSD) ranged from 5.6 to 7.5%. The method has been applied effectively to analyze the content of phthalates in 35 food wrap samples that were collected from markets in Hanoi. Analytical results showed that ten samples were detected with phthalates, of which three samples contained DEHA exceeding the specific migration limit (SML). This study contributes to consumer health protection and advises the regulatory agency on the addition of safety regulations for PVC food wrap in particular and other food packaging materials in general.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89998293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.47866/2615-9252/vjfc.4046
Ngan Tong Thi, Thanh Mai Ngọc, Hien Nguyen Thi, Quyen Pham Thi, Dan Nguyen Phuc, Huyen Nguyen Thi, Minh Hoa Nguyen Thi, Lam Vu Tung, Thuy Chu Thi, Lan Anh Hoang Thi, Anh Huong Nguyen Thi
Isocyanate is a common name for chemical compounds containing one or more - NCO groups. There have been many studies showing that isocyanates pose a risk to human health when exposed and can cause occupational poisoning. When absorbed into the body, the isocyanates will be converted into the corresponding amines. Therefore, the determination of these diamines in urine will contribute to the assessment of exposure to isocyanates. This article presents a study on simultaneous determination of 4,4′-methylenedianiline (MDA), hexamethylene diamine (HDA), isophoron diamine (IPDA) in urine by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method detection limit (MDL) were 0.074, 0.059, and 0.053 ng/mL for HDA, MDA, IPDA, respectively. The method quantitation limit (MQL) were 0.243, 0.194 and 0.177 ng/mL for HDA, MDA, IPDA, respectively. The method's recovery ranged from 86.38 to 105.3% with the repeatability RSDr < 6%. The method was successfully applied to simultaneously determine the content of HDA, MDA and IPDA in 30 urine samples of workers at auto repair garages in Bac Ninh province. The results showed that HDA, MDA and IPDA were detected in these samples with different concentrations.
{"title":"Study on the determination of some diamine in urine by LC-MS/MS","authors":"Ngan Tong Thi, Thanh Mai Ngọc, Hien Nguyen Thi, Quyen Pham Thi, Dan Nguyen Phuc, Huyen Nguyen Thi, Minh Hoa Nguyen Thi, Lam Vu Tung, Thuy Chu Thi, Lan Anh Hoang Thi, Anh Huong Nguyen Thi","doi":"10.47866/2615-9252/vjfc.4046","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4046","url":null,"abstract":"Isocyanate is a common name for chemical compounds containing one or more - NCO groups. There have been many studies showing that isocyanates pose a risk to human health when exposed and can cause occupational poisoning. When absorbed into the body, the isocyanates will be converted into the corresponding amines. Therefore, the determination of these diamines in urine will contribute to the assessment of exposure to isocyanates. This article presents a study on simultaneous determination of 4,4′-methylenedianiline (MDA), hexamethylene diamine (HDA), isophoron diamine (IPDA) in urine by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method detection limit (MDL) were 0.074, 0.059, and 0.053 ng/mL for HDA, MDA, IPDA, respectively. The method quantitation limit (MQL) were 0.243, 0.194 and 0.177 ng/mL for HDA, MDA, IPDA, respectively. The method's recovery ranged from 86.38 to 105.3% with the repeatability RSDr < 6%. The method was successfully applied to simultaneously determine the content of HDA, MDA and IPDA in 30 urine samples of workers at auto repair garages in Bac Ninh province. The results showed that HDA, MDA and IPDA were detected in these samples with different concentrations.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87419119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.47866/2615-9252/vjfc.4045
Hien Dang Thu, Lan Phuong Kieu Thi, Thu Hang Do Thi
Liquid chromatography - high resolution mass spectrometry (LC-HRMS) developed to screen some residues in rice. Pesticides, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2 and quaternary ammonium surfactants were detected by the optimized method. Rice samples were extracted and cleaned by QuEChERS then analyzed by QExactive system with HESI ionization source for both negative and positive modes. The C18 reversed phase column was used and the mobile phase consists of water and Methanol containing 0.1% formic acid and 10 mM ammonium formate. The method was applied successfully to analyze 60 rice samples collected in Hanoi city. The results showed that 5 pesticides in 30 rice samples from 10.3 to 160 µg/kg.
{"title":"Development of method for screening and quantification of some contaminants in rice","authors":"Hien Dang Thu, Lan Phuong Kieu Thi, Thu Hang Do Thi","doi":"10.47866/2615-9252/vjfc.4045","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4045","url":null,"abstract":"Liquid chromatography - high resolution mass spectrometry (LC-HRMS) developed to screen some residues in rice. Pesticides, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2 and quaternary ammonium surfactants were detected by the optimized method. Rice samples were extracted and cleaned by QuEChERS then analyzed by QExactive system with HESI ionization source for both negative and positive modes. The C18 reversed phase column was used and the mobile phase consists of water and Methanol containing 0.1% formic acid and 10 mM ammonium formate. The method was applied successfully to analyze 60 rice samples collected in Hanoi city. The results showed that 5 pesticides in 30 rice samples from 10.3 to 160 µg/kg. ","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":"31 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87663444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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