Because the adhesives usually consist of the multicomponents containing the polymer, the knowledge such as not only the miscibility between components but also the structure and properties in the adhesion interface is very important. We measured the interfacial thickness and the concentration profile between different polymers by using an ellipsometry and some methods of microscopy, and considered these from the viewpoint of miscibility. And, in order to investigate the initial formation process of the interface, the behavior of interfacial region was measured by a high-speed ellipsometry, whose measuring-time-interval was very short. It was considered that high mobility and entropic elasticity of the interfacial molecules contributed to the formation of the interfacial region on the initial stage. Moreover, the adhesion strength was measured in non-reactive and reactive systems and the relation between interfacial structure and adhesion strength was discussed.
{"title":"Interfacial Structure between Different Polymers and its Adhesion","authors":"T. Ougizawa","doi":"10.1380/JSSSJ.38.55","DOIUrl":"https://doi.org/10.1380/JSSSJ.38.55","url":null,"abstract":"Because the adhesives usually consist of the multicomponents containing the polymer, the knowledge such as not only the miscibility between components but also the structure and properties in the adhesion interface is very important. We measured the interfacial thickness and the concentration profile between different polymers by using an ellipsometry and some methods of microscopy, and considered these from the viewpoint of miscibility. And, in order to investigate the initial formation process of the interface, the behavior of interfacial region was measured by a high-speed ellipsometry, whose measuring-time-interval was very short. It was considered that high mobility and entropic elasticity of the interfacial molecules contributed to the formation of the interfacial region on the initial stage. Moreover, the adhesion strength was measured in non-reactive and reactive systems and the relation between interfacial structure and adhesion strength was discussed.","PeriodicalId":13075,"journal":{"name":"Hyomen Kagaku","volume":"43 1","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73541188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The identification of antigen that induces activation of mast cells and basophils in a patient is crucial to avoid symptoms of allergic diseases. Since surface plasmon resonance imaging (SPRI) can visualize activation of individual cell, such as basophils without any labeling, SPRI sensor possesses a great potential for clinical diagnosis of allergy. However, conventional technique of SPRI requires freshly isolated basophils of patients and cannot analyze multiple samples in parallel. To overcome such problems, we developed devices for SPRI to make a broad observation area and a multi-well SPRI sensor chip with a hydrophobic membrane. The employment of human IgE receptor-expressing mast cell lines (RBL-48 cells) sensitized with serum, collected and stored from less than a microliter of patient ʼ s blood, allowed us to detect specific reactions of RBL-48 cells in response to antigens, suggesting this technique may be a useful tool as a screening system of type I allergy.
{"title":"Development of Allergy Test by SPR Imaging Sensor with a Drop of Patientʼs Serum","authors":"Y. Yanase","doi":"10.1380/jsssj.38.390","DOIUrl":"https://doi.org/10.1380/jsssj.38.390","url":null,"abstract":"The identification of antigen that induces activation of mast cells and basophils in a patient is crucial to avoid symptoms of allergic diseases. Since surface plasmon resonance imaging (SPRI) can visualize activation of individual cell, such as basophils without any labeling, SPRI sensor possesses a great potential for clinical diagnosis of allergy. However, conventional technique of SPRI requires freshly isolated basophils of patients and cannot analyze multiple samples in parallel. To overcome such problems, we developed devices for SPRI to make a broad observation area and a multi-well SPRI sensor chip with a hydrophobic membrane. The employment of human IgE receptor-expressing mast cell lines (RBL-48 cells) sensitized with serum, collected and stored from less than a microliter of patient ʼ s blood, allowed us to detect specific reactions of RBL-48 cells in response to antigens, suggesting this technique may be a useful tool as a screening system of type I allergy.","PeriodicalId":13075,"journal":{"name":"Hyomen Kagaku","volume":"83 1","pages":"390-394"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73671677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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