Pub Date : 2021-01-01DOI: 10.37747/10.37747/2312-640x-2021-19-155-158
E.I. Ivanova, V.O. Grinin, A. Bakulina, P. S. Timashev
In this work, a program was developed based on U-Net and VGG neural network architectures for segmentation and analysis of scan images of kidney and colon tissues. The program maps up to 12 classes of anatomical structures in tissues, and also allows the calculation of predictive histological biomarkers to support physician decision-making.
{"title":"ARTIFICIAL INTELLIGENCE TECHNOLOGIES FOR MORPHOLOGY: OPPORTUNITIES AND PROSPECTS","authors":"E.I. Ivanova, V.O. Grinin, A. Bakulina, P. S. Timashev","doi":"10.37747/10.37747/2312-640x-2021-19-155-158","DOIUrl":"https://doi.org/10.37747/10.37747/2312-640x-2021-19-155-158","url":null,"abstract":"In this work, a program was developed based on U-Net and VGG neural network architectures for segmentation and analysis of scan images of kidney and colon tissues. The program maps up to 12 classes of anatomical structures in tissues, and also allows the calculation of predictive histological biomarkers to support physician decision-making.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79928499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-246-248
P. Semizhon, H.P. Scheslenok, I. V. Harbachova, I. Vashkevich, O. Sviridov
To develop the technologies of beta-lactam antibiotics bioassays in foods, high purity beta-lactam receptor BlaR-CTD has been obtained and characterized. A test system for the quantitation of the biologically active and immunoreactive receptor in the processes of its heterological expression, isolation and reagent forms preparation was elaborated. The immunochemical properties and antibiotic binding ability of BlaR-CTD were investigated, and the constructions and application procedures of receptor-immune systems for the assay of beta-lactam antibiotics using microplates or chromatographic test-strips were proposed.
{"title":"MONITORING OF BIOLOGICAL ACTIVITY OF BETA-LACTAM ANTIBIOTICS RECEPTOR BLAR-CTD IN THE PROCESSES OF ITS HETEROLOGICAL EXPRESSION, PURIFICATION AND APPLICATION IN ANALYTICAL SYSTEMS","authors":"P. Semizhon, H.P. Scheslenok, I. V. Harbachova, I. Vashkevich, O. Sviridov","doi":"10.37747/2312-640x-2021-19-246-248","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-246-248","url":null,"abstract":"To develop the technologies of beta-lactam antibiotics bioassays in foods, high purity beta-lactam receptor BlaR-CTD has been obtained and characterized. A test system for the quantitation of the biologically active and immunoreactive receptor in the processes of its heterological expression, isolation and reagent forms preparation was elaborated. The immunochemical properties and antibiotic binding ability of BlaR-CTD were investigated, and the constructions and application procedures of receptor-immune systems for the assay of beta-lactam antibiotics using microplates or chromatographic test-strips were proposed.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"152 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91493108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-18-20
N. Shved, O.Yu. Kuzyakova, A.V. Vitkalova, V. Kovalev, G. Malykin, I. Dyuizen, V. V. Kumeiko
The structure and properties of implanted materials based on modified pectins have been investigated, and the proposed mechanism of their action on the cells of the nervous tissue has been shown.
研究了基于改性果胶的植入材料的结构和性能，并揭示了其对神经组织细胞的作用机制。
{"title":"IMPLANTED MATERIALS BASED ON MODIFIED PECTINS FOR BIOENGINEERING NERVE TISSUE RECONSTRUCTION","authors":"N. Shved, O.Yu. Kuzyakova, A.V. Vitkalova, V. Kovalev, G. Malykin, I. Dyuizen, V. V. Kumeiko","doi":"10.37747/2312-640x-2021-19-18-20","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-18-20","url":null,"abstract":"The structure and properties of implanted materials based on modified pectins have been investigated, and the proposed mechanism of their action on the cells of the nervous tissue has been shown.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91339710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-322-324
E. Zvereva, A. Zherdev, B. Dzantiev
Methods have been developed to control the content of non-meat components (connective tissue of animals, eggs, soy) in processed meat products, based on enzyme immunoassay of biomarker proteins – collagen, ovalbumin, soybean trypsin inhibitor.
{"title":"DEVELOPMENT OF IMMUNOENZYME ANALYTICAL SYSTEMS FOR CONTROL OF THE CONTENT OF MAIN NON-MEAT COMPOUNDS IN PROCESSED MEAT PRODUCTS","authors":"E. Zvereva, A. Zherdev, B. Dzantiev","doi":"10.37747/2312-640x-2021-19-322-324","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-322-324","url":null,"abstract":"Methods have been developed to control the content of non-meat components (connective tissue of animals, eggs, soy) in processed meat products, based on enzyme immunoassay of biomarker proteins – collagen, ovalbumin, soybean trypsin inhibitor.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81300430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-69-70
Y. Zorkina, E. Zubkov, V. Ushakova, A. Morozova
Prenatal stress induced by exposure to variable ultrasound has been demonstrated to induce anxious behavior and change social preferences in the rat offspring at juvenile age.
暴露于可变超声诱发的产前应激已被证明可诱发幼年大鼠后代的焦虑行为和改变社会偏好。
{"title":"EARLY BEHAVIORAL DISORDERS IN RATS UNDER THE INFLUENCE OF CHRONIC PRENATAL STRESS","authors":"Y. Zorkina, E. Zubkov, V. Ushakova, A. Morozova","doi":"10.37747/2312-640x-2021-19-69-70","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-69-70","url":null,"abstract":"Prenatal stress induced by exposure to variable ultrasound has been demonstrated to induce anxious behavior and change social preferences in the rat offspring at juvenile age.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81323160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-29-31
M. Khlupova, G. Shumakovich, E. Zaitseva, E. Gromova, O. Morozova
Enzymatic synthesis of polyaniline (PANI) doped with bivalent copper ions was carried out on a sodium polystyrene sulfonate (PSS) template. The synthesized interpolymer complex PANI-Cu2+/PSS had an inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli cells.
{"title":"ANTIMICROBIAL FILM COATING BASED ON ENZYMATICALLY SYNTHESIZED POLYANILINE DOPED WITH COPPER IONS","authors":"M. Khlupova, G. Shumakovich, E. Zaitseva, E. Gromova, O. Morozova","doi":"10.37747/2312-640x-2021-19-29-31","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-29-31","url":null,"abstract":"Enzymatic synthesis of polyaniline (PANI) doped with bivalent copper ions was carried out on a sodium polystyrene sulfonate (PSS) template. The synthesized interpolymer complex PANI-Cu2+/PSS had an inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli cells.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81478874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-331-332
S. Belenkaya, V. V. Elchaninov
A plasmid vector pSVB was developed, which allows integrating an expression cassette containing the maral prochymosin gene into the genome of the yeast Kluyveromyces lactis. Producer strains have been obtained that ensure the accumulation of recombinant maral chymosin in the culture medium with a milk-clotting activity of up to 145 AU / ml.
建立了一种质粒载体pSVB，将含有酵母原凝乳酶基因的表达盒整合到酵母的基因组中。已经获得了生产菌株，确保在培养基中积累重组马尔凝乳酶，凝乳活性高达145 AU / ml。
{"title":"DEVELOPMENT OF A PRODUCER OF RECOMBINANT MARAL CHYMOSIN BASED ON THE YEAST KLUYVEROMYCES LACTIS","authors":"S. Belenkaya, V. V. Elchaninov","doi":"10.37747/2312-640x-2021-19-331-332","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-331-332","url":null,"abstract":"A plasmid vector pSVB was developed, which allows integrating an expression cassette containing the maral prochymosin gene into the genome of the yeast Kluyveromyces lactis. Producer strains have been obtained that ensure the accumulation of recombinant maral chymosin in the culture medium with a milk-clotting activity of up to 145 AU / ml.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85161838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-185-187
I. V. Kazeev, I. Khudeev, O. Bocharova
The paper presents the developing direction of supercritical extraction (SCE), which opens up new prospects for the extraction of biologically active substances from plant raw materials. Research is underway to obtain active biological substances for the treatment of socially significant diseases.
{"title":"SUPERCRITICAL EXTRACTION FOR THE ISOLATION OF BIOLOGICALLY ACTIVE SUBSTANCES","authors":"I. V. Kazeev, I. Khudeev, O. Bocharova","doi":"10.37747/2312-640x-2021-19-185-187","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-185-187","url":null,"abstract":"The paper presents the developing direction of supercritical extraction (SCE), which opens up new prospects for the extraction of biologically active substances from plant raw materials. Research is underway to obtain active biological substances for the treatment of socially significant diseases.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87800530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-22-24
A. Nechaev, P. Eremin, I. Gilmutdinova
The paper presents the results of the development of a bioactive bioplastic material (BIO) based on ion-track wound coatings (ITRP) and a nano-scaffold of chitosan obtained by electroforming. The water and gas permeability, the ultimate strength, bacteriostaticity, cytotoxicity and biocompatibility of BIO ITPR were investigated.
{"title":"BIOACTIVE BIOPLASTIC MATERIAL BASED ON ION-TRACK WOUND COATINGS AND CHITOSAN NANO-SCAFFOLD","authors":"A. Nechaev, P. Eremin, I. Gilmutdinova","doi":"10.37747/2312-640x-2021-19-22-24","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-22-24","url":null,"abstract":"The paper presents the results of the development of a bioactive bioplastic material (BIO) based on ion-track wound coatings (ITRP) and a nano-scaffold of chitosan obtained by electroforming. The water and gas permeability, the ultimate strength, bacteriostaticity, cytotoxicity and biocompatibility of BIO ITPR were investigated.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88108109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-316-317
T. Leonova, T. I. Kalinina, T. V. Gerasimova, I. Titov, A. Yanenko
A point mutation in the regulatory region of the ilvBNC operon leads to an increase in the transcription of the operon genes, an increase in the activity of the AHAS enzyme, and an increase in the synthesis of L-valine.
{"title":"EFFECT OF MUTATIONS IN THE REGULATORY REGION OF OPERON ILVBNC ON EXPRESSION OF OPERON GENES, ACTIVITY OF THE ENZYME AHAS AND PRODUCTION OF L-VALINE IN CORYNEBACTERIUM GLUTAMICUM STRAINS","authors":"T. Leonova, T. I. Kalinina, T. V. Gerasimova, I. Titov, A. Yanenko","doi":"10.37747/2312-640x-2021-19-316-317","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-316-317","url":null,"abstract":"A point mutation in the regulatory region of the ilvBNC operon leads to an increase in the transcription of the operon genes, an increase in the activity of the AHAS enzyme, and an increase in the synthesis of L-valine.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87151263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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