Pub Date : 2021-01-01DOI: 10.37747/10.37747/2312-640x-2021-19-155-158
E.I. Ivanova, V.O. Grinin, A. Bakulina, P. S. Timashev
In this work, a program was developed based on U-Net and VGG neural network architectures for segmentation and analysis of scan images of kidney and colon tissues. The program maps up to 12 classes of anatomical structures in tissues, and also allows the calculation of predictive histological biomarkers to support physician decision-making.
{"title":"ARTIFICIAL INTELLIGENCE TECHNOLOGIES FOR MORPHOLOGY: OPPORTUNITIES AND PROSPECTS","authors":"E.I. Ivanova, V.O. Grinin, A. Bakulina, P. S. Timashev","doi":"10.37747/10.37747/2312-640x-2021-19-155-158","DOIUrl":"https://doi.org/10.37747/10.37747/2312-640x-2021-19-155-158","url":null,"abstract":"In this work, a program was developed based on U-Net and VGG neural network architectures for segmentation and analysis of scan images of kidney and colon tissues. The program maps up to 12 classes of anatomical structures in tissues, and also allows the calculation of predictive histological biomarkers to support physician decision-making.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79928499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-246-248
P. Semizhon, H.P. Scheslenok, I. V. Harbachova, I. Vashkevich, O. Sviridov
To develop the technologies of beta-lactam antibiotics bioassays in foods, high purity beta-lactam receptor BlaR-CTD has been obtained and characterized. A test system for the quantitation of the biologically active and immunoreactive receptor in the processes of its heterological expression, isolation and reagent forms preparation was elaborated. The immunochemical properties and antibiotic binding ability of BlaR-CTD were investigated, and the constructions and application procedures of receptor-immune systems for the assay of beta-lactam antibiotics using microplates or chromatographic test-strips were proposed.
{"title":"MONITORING OF BIOLOGICAL ACTIVITY OF BETA-LACTAM ANTIBIOTICS RECEPTOR BLAR-CTD IN THE PROCESSES OF ITS HETEROLOGICAL EXPRESSION, PURIFICATION AND APPLICATION IN ANALYTICAL SYSTEMS","authors":"P. Semizhon, H.P. Scheslenok, I. V. Harbachova, I. Vashkevich, O. Sviridov","doi":"10.37747/2312-640x-2021-19-246-248","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-246-248","url":null,"abstract":"To develop the technologies of beta-lactam antibiotics bioassays in foods, high purity beta-lactam receptor BlaR-CTD has been obtained and characterized. A test system for the quantitation of the biologically active and immunoreactive receptor in the processes of its heterological expression, isolation and reagent forms preparation was elaborated. The immunochemical properties and antibiotic binding ability of BlaR-CTD were investigated, and the constructions and application procedures of receptor-immune systems for the assay of beta-lactam antibiotics using microplates or chromatographic test-strips were proposed.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"152 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91493108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-18-20
N. Shved, O.Yu. Kuzyakova, A.V. Vitkalova, V. Kovalev, G. Malykin, I. Dyuizen, V. V. Kumeiko
The structure and properties of implanted materials based on modified pectins have been investigated, and the proposed mechanism of their action on the cells of the nervous tissue has been shown.
研究了基于改性果胶的植入材料的结构和性能，并揭示了其对神经组织细胞的作用机制。
{"title":"IMPLANTED MATERIALS BASED ON MODIFIED PECTINS FOR BIOENGINEERING NERVE TISSUE RECONSTRUCTION","authors":"N. Shved, O.Yu. Kuzyakova, A.V. Vitkalova, V. Kovalev, G. Malykin, I. Dyuizen, V. V. Kumeiko","doi":"10.37747/2312-640x-2021-19-18-20","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-18-20","url":null,"abstract":"The structure and properties of implanted materials based on modified pectins have been investigated, and the proposed mechanism of their action on the cells of the nervous tissue has been shown.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91339710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-383-385
D.M. Smolyaninova, E. V. Bizina, M. Larina, T. Ermolaeva
Piezoelectric gravimetric sensors based on magnetic nanocomposites: polymers with molecular imprints (MIP) Fe3O4@MIP N - (phosphonmethyl) - glycine have been developed. It was found that the optimal concentration ratio of template: functional monomer: cross-monomer is 1: 3: 25. The best synthesis time is 3h with constant stirring at a speed of 60 rpm and heating to 60 °C.
基于磁性纳米复合材料的压电式重力传感器:分子印迹聚合物(MIP) Fe3O4@MIP N -(磷甲基)-甘氨酸。结果表明，模板、功能单体、交叉单体的最佳浓度比为1:3:25。最佳合成时间为3h，转速为60rpm，加热至60℃。
{"title":"MAGNETIC NANOCOMPOSITES IN PIEZOELECTRIC SENSORS FOR DETERMINATION OF GLYPHOSATE","authors":"D.M. Smolyaninova, E. V. Bizina, M. Larina, T. Ermolaeva","doi":"10.37747/2312-640x-2021-19-383-385","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-383-385","url":null,"abstract":"Piezoelectric gravimetric sensors based on magnetic nanocomposites: polymers with molecular imprints (MIP) Fe3O4@MIP N - (phosphonmethyl) - glycine have been developed. It was found that the optimal concentration ratio of template: functional monomer: cross-monomer is 1: 3: 25. The best synthesis time is 3h with constant stirring at a speed of 60 rpm and heating to 60 °C.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73354264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-236-238
G. Kutdusova, E. Suprun
Deoxyuridine triphosphates modified with tyrosine or tryptophan aromatic groups were tested as electroactive ‘labels’ for the electrochemical detection of double-stranded DNA amplicons obtained by recombinase polymerase amplification.
{"title":"DEOXYURIDINE TRIPHOSPHATES MODIFIED WITH AROMATIC TYROSINE GROUPS FOR ELECTROCHEMICAL DETERMINATION OF RECOMBINASE POLYMERASE AMPLIFICATION PRODUCTS OF DNA","authors":"G. Kutdusova, E. Suprun","doi":"10.37747/2312-640x-2021-19-236-238","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-236-238","url":null,"abstract":"Deoxyuridine triphosphates modified with tyrosine or tryptophan aromatic groups were tested as electroactive ‘labels’ for the electrochemical detection of double-stranded DNA amplicons obtained by recombinase polymerase amplification.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80155478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-29-31
M. Khlupova, G. Shumakovich, E. Zaitseva, E. Gromova, O. Morozova
Enzymatic synthesis of polyaniline (PANI) doped with bivalent copper ions was carried out on a sodium polystyrene sulfonate (PSS) template. The synthesized interpolymer complex PANI-Cu2+/PSS had an inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli cells.
{"title":"ANTIMICROBIAL FILM COATING BASED ON ENZYMATICALLY SYNTHESIZED POLYANILINE DOPED WITH COPPER IONS","authors":"M. Khlupova, G. Shumakovich, E. Zaitseva, E. Gromova, O. Morozova","doi":"10.37747/2312-640x-2021-19-29-31","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-29-31","url":null,"abstract":"Enzymatic synthesis of polyaniline (PANI) doped with bivalent copper ions was carried out on a sodium polystyrene sulfonate (PSS) template. The synthesized interpolymer complex PANI-Cu2+/PSS had an inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli cells.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81478874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-142-144
V. Skripova, L. Bulatova, D. Savenkova, A. K. Nurgalieva, R. Vlasenkova, D. Reshetnikova, A. Timonina, M. Bogdanov
Using CRISPR/Cas9 and SEREX technologies we have identified and characterized more than two hundred potential molecular markers of oncological diseases. It was shown that the sodium-dependent phosphate transporter NaPi2b is a unique target for the development of antitumor targeted drugs due to its localization and tumor-specific epitope.
{"title":"MOLECULAR MARKERS FOR CANCER DIAGNOSIS AND THERAPY","authors":"V. Skripova, L. Bulatova, D. Savenkova, A. K. Nurgalieva, R. Vlasenkova, D. Reshetnikova, A. Timonina, M. Bogdanov","doi":"10.37747/2312-640x-2021-19-142-144","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-142-144","url":null,"abstract":"Using CRISPR/Cas9 and SEREX technologies we have identified and characterized more than two hundred potential molecular markers of oncological diseases. It was shown that the sodium-dependent phosphate transporter NaPi2b is a unique target for the development of antitumor targeted drugs due to its localization and tumor-specific epitope.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82579207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-244-246
O. Bodulev, A. Soloviev
This work will present the results of the development of two new highly sensitive heterogeneous methods for the determination of miRNA-141 and miRNA-39 using the isothermal circular strand-displacement amplification and the catalytic hairpin assembly methods for analyte amplification.
{"title":"HIGHLY SENSITIVE METHODS FOR MICRORNA DETECTION USING ISOTHERMAL METHODS FOR NUCLEIC ACIDS AMPLIFICATION","authors":"O. Bodulev, A. Soloviev","doi":"10.37747/2312-640x-2021-19-244-246","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-244-246","url":null,"abstract":"This work will present the results of the development of two new highly sensitive heterogeneous methods for the determination of miRNA-141 and miRNA-39 using the isothermal circular strand-displacement amplification and the catalytic hairpin assembly methods for analyte amplification.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80879557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-316-317
T. Leonova, T. I. Kalinina, T. V. Gerasimova, I. Titov, A. Yanenko
A point mutation in the regulatory region of the ilvBNC operon leads to an increase in the transcription of the operon genes, an increase in the activity of the AHAS enzyme, and an increase in the synthesis of L-valine.
{"title":"EFFECT OF MUTATIONS IN THE REGULATORY REGION OF OPERON ILVBNC ON EXPRESSION OF OPERON GENES, ACTIVITY OF THE ENZYME AHAS AND PRODUCTION OF L-VALINE IN CORYNEBACTERIUM GLUTAMICUM STRAINS","authors":"T. Leonova, T. I. Kalinina, T. V. Gerasimova, I. Titov, A. Yanenko","doi":"10.37747/2312-640x-2021-19-316-317","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-316-317","url":null,"abstract":"A point mutation in the regulatory region of the ilvBNC operon leads to an increase in the transcription of the operon genes, an increase in the activity of the AHAS enzyme, and an increase in the synthesis of L-valine.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87151263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-185-187
I. V. Kazeev, I. Khudeev, O. Bocharova
The paper presents the developing direction of supercritical extraction (SCE), which opens up new prospects for the extraction of biologically active substances from plant raw materials. Research is underway to obtain active biological substances for the treatment of socially significant diseases.
{"title":"SUPERCRITICAL EXTRACTION FOR THE ISOLATION OF BIOLOGICALLY ACTIVE SUBSTANCES","authors":"I. V. Kazeev, I. Khudeev, O. Bocharova","doi":"10.37747/2312-640x-2021-19-185-187","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-185-187","url":null,"abstract":"The paper presents the developing direction of supercritical extraction (SCE), which opens up new prospects for the extraction of biologically active substances from plant raw materials. Research is underway to obtain active biological substances for the treatment of socially significant diseases.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87800530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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