Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-232-234
I. A. Shanin, M. Karimova, L. Mukhametova
Reagents for fluorescence polarization immunoassay (FPIA) of phthalates have been obtained: phthalate-protein conjugates, polyclonal antibodies, tracers - antigens labeled with fluorescein derivatives. By the example of dibutyl phthalate (DBP) with the use of a portable detector Sentry-200, the FPIA method was developed, which makes it possible to determine DBP in water samples in concentrations 1 μg / ml in 5 min.
{"title":"DEVELOPMENT OF FLUORESCENCE POLARIZATION IMMUNOASSAY OF PHTHALATES: COMPARISON OF DIFFERENT DERIVATIVES IN OBTAINING ANTIBODIES AND TRACERS","authors":"I. A. Shanin, M. Karimova, L. Mukhametova","doi":"10.37747/2312-640x-2021-19-232-234","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-232-234","url":null,"abstract":"Reagents for fluorescence polarization immunoassay (FPIA) of phthalates have been obtained: phthalate-protein conjugates, polyclonal antibodies, tracers - antigens labeled with fluorescein derivatives. By the example of dibutyl phthalate (DBP) with the use of a portable detector Sentry-200, the FPIA method was developed, which makes it possible to determine DBP in water samples in concentrations 1 μg / ml in 5 min.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84152218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-284-286
V. Revin, E. Liyaskina, N. Pestov, D. Kadimaliev, A.B. Dolganov
New generation polyfunctional materials based on such microbial polysaccharides as bacterial cellulose, alginate, xanthan, chitosan have been obtained. The possibility of their application as materials with antibacterial, regenerative and haemostatic properties, ultra-low thermal conductivity, density, high noise-absorbing and sorption properties has been shown.
{"title":"MICROBIAL POLYSACCHARIDES – THE BASIS FOR NEW GENERATION POLYFUNCTIONAL MATERIALS","authors":"V. Revin, E. Liyaskina, N. Pestov, D. Kadimaliev, A.B. Dolganov","doi":"10.37747/2312-640x-2021-19-284-286","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-284-286","url":null,"abstract":"New generation polyfunctional materials based on such microbial polysaccharides as bacterial cellulose, alginate, xanthan, chitosan have been obtained. The possibility of their application as materials with antibacterial, regenerative and haemostatic properties, ultra-low thermal conductivity, density, high noise-absorbing and sorption properties has been shown.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76361212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-52-53
Е.А. Stekolshchikova, А.I. Tkachev, М.S. Osetrova, D. Smirnov, О.I. Efimova
We measured lipid composition for 33 regions of the brain, covering all basic brain structures in healthy humans, chimpanzees, bonobos, and macaques. This analysis allows us to identify features of lipidomes of functionally divergent brain regions that are unique to humans.
{"title":"EVOLUTION OF THE LIPID COMPOSITION OF THE HUMAN BRAIN","authors":"Е.А. Stekolshchikova, А.I. Tkachev, М.S. Osetrova, D. Smirnov, О.I. Efimova","doi":"10.37747/2312-640x-2021-19-52-53","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-52-53","url":null,"abstract":"We measured lipid composition for 33 regions of the brain, covering all basic brain structures in healthy humans, chimpanzees, bonobos, and macaques. This analysis allows us to identify features of lipidomes of functionally divergent brain regions that are unique to humans.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76362047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-255-257
A. Lyapina, M. Khizhnyakova, N. V. Kichemazova, A. Kolosova, V. Evstifeev, O. Larionova, V. Feodorova
The results of dot-ELISA application for the detection of IgG specific to the recombinant Chlamydia psittaci DnaK antigen in sera of farm animals vaccinated with a commercial chlamydia vaccine are presented. It was revealed, that C. psittaci heat shock protein DnaK participates in immune response of sheep immunized with the vaccine based on the C. psittaci Rostinovo-70 strain.
{"title":"DETECTION OF SEROLOGICAL RESPONSE TO THE RECOMBINANT CHLAMYDIA PSITTACI PROTEIN DNAK USING DOT-ELISA TECHNIQUE","authors":"A. Lyapina, M. Khizhnyakova, N. V. Kichemazova, A. Kolosova, V. Evstifeev, O. Larionova, V. Feodorova","doi":"10.37747/2312-640x-2021-19-255-257","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-255-257","url":null,"abstract":"The results of dot-ELISA application for the detection of IgG specific to the recombinant Chlamydia psittaci DnaK antigen in sera of farm animals vaccinated with a commercial chlamydia vaccine are presented. It was revealed, that C. psittaci heat shock protein DnaK participates in immune response of sheep immunized with the vaccine based on the C. psittaci Rostinovo-70 strain.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76553117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-163-166
E. A. Kochina, É. A. Mikhailyuk, D. Glazkova, E. V. Bogoslovskaya
Here we present results of a comparison of two methods of purification of adeno-associated virus: iodixanol gradient ultracentrifugation and polyethylene glycol solution (PEG 8000) two-stage purification.
{"title":"EVALUATION OF VIRAL PARTICLES YIELD AT DIFFERENT STAGES OF ADENO-ASSOCIATED VIRUS PURIFICATION","authors":"E. A. Kochina, É. A. Mikhailyuk, D. Glazkova, E. V. Bogoslovskaya","doi":"10.37747/2312-640x-2021-19-163-166","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-163-166","url":null,"abstract":"Here we present results of a comparison of two methods of purification of adeno-associated virus: iodixanol gradient ultracentrifugation and polyethylene glycol solution (PEG 8000) two-stage purification.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90243133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-376-377
S. Volodina, O. V. Topkova
The work is devoted to the study of the processes of callusogenesis of Vitex agnus-castus in vitro. The ability of callus cell culture to biosynthesis of ecdysteroids is shown.
研究牡荆离体愈伤组织发生的过程。愈伤组织细胞培养具有生物合成外皮甾体的能力。
{"title":"OBTAINING CALLUS CULTURE OF VITEX AGNUS-CASTUS CELLS AS A PRODUCER FOR BIOTECHNOLOGICAL OBTAINING OF PHYTOEXDYSTEROIDS","authors":"S. Volodina, O. V. Topkova","doi":"10.37747/2312-640x-2021-19-376-377","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-376-377","url":null,"abstract":"The work is devoted to the study of the processes of callusogenesis of Vitex agnus-castus in vitro. The ability of callus cell culture to biosynthesis of ecdysteroids is shown.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88842301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-187-189
G. Rudnitskaya, T. Lukashenko, A. Evstrapov
Microchip devices made of glass, silicon, polymeric materials have been designed and tested for use in Point-of- Care diagnostic systems for biomedical purposes.
由玻璃、硅、聚合物材料制成的微芯片设备已被设计和测试用于生物医学目的的即时护理诊断系统。
{"title":"POINT-OF-CARE (POC) DEVICES – A NEW TREND IN BIOMEDICAL DEVICE","authors":"G. Rudnitskaya, T. Lukashenko, A. Evstrapov","doi":"10.37747/2312-640x-2021-19-187-189","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-187-189","url":null,"abstract":"Microchip devices made of glass, silicon, polymeric materials have been designed and tested for use in Point-of- Care diagnostic systems for biomedical purposes.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88719245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-141-142
D. V. Petrovsky
Proteomic (MASCOT, Andromeda, OMSSA, etc.) and metabolomic (Scripps, AMDIS, etc.) search algorithms for identification are limited to reference libraries and, as a result, a large amount of data is lost (modified proteins, isoforms). Bypassing the identification stage, you can significantly increase the amount of data for classification tasks. This makes it possible to successfully cluster the studied classes at the cost of information on molecular categorical predictors.
{"title":"NEURAL NETWORK AS AN APPROACH TO THE CLASSIFICATION OF UNASSIGNED LC-MS DATA IN PROTEOMETABOLOMICS","authors":"D. V. Petrovsky","doi":"10.37747/2312-640x-2021-19-141-142","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-141-142","url":null,"abstract":"Proteomic (MASCOT, Andromeda, OMSSA, etc.) and metabolomic (Scripps, AMDIS, etc.) search algorithms for identification are limited to reference libraries and, as a result, a large amount of data is lost (modified proteins, isoforms). Bypassing the identification stage, you can significantly increase the amount of data for classification tasks. This makes it possible to successfully cluster the studied classes at the cost of information on molecular categorical predictors.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"337 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76584490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-335-338
V. Kalashnikov, L. Khrabrova, N. Blokhina, T. Kalashnikova
For the first time, a genome-wide study was conducted for 6 native breeds (Kabardian, Vyatka, zabaikalian, Yakut) with unique adaptive qualities and 2 cultural breeds that are strictly selected for multidirectional characteristics. Data on the state of the genetic structure of breeds were obtained. The phylogenetic relationships between breeds were studied, the ancestral groups that participated in the formation of the breed structure were determined, and the influence of factory improver breeds on the microevolution of local breeds was estimated. the results of the research are correlated with the freely available results of the genome-wide analysis of foreign horse populations and breeds. The ways of identifying complexes that characterize the severity of selected traits in breeds with different directions of use are outlined. The high efficiency of using the obtained results to determine belonging to a particular breed is determined. A database of mitochondrial DNA and Y-chromosome haplotypes was created to conduct a retrospective analysis of the origin and refinement of breeding records in the system of centralized breeding records in horse breeding. As additional markers, we used targeted SNPs that characterize the severity of selected traits
{"title":"A STUDY OF THE POPULATION GENOMIC STRUCTURE AND THE CHARACTER OF THE GENETIC DIVERGENCE OF EQUINE BREEDS (EQUUS CABALLUS L.)","authors":"V. Kalashnikov, L. Khrabrova, N. Blokhina, T. Kalashnikova","doi":"10.37747/2312-640x-2021-19-335-338","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-335-338","url":null,"abstract":"For the first time, a genome-wide study was conducted for 6 native breeds (Kabardian, Vyatka, zabaikalian, Yakut) with unique adaptive qualities and 2 cultural breeds that are strictly selected for multidirectional characteristics. Data on the state of the genetic structure of breeds were obtained. The phylogenetic relationships between breeds were studied, the ancestral groups that participated in the formation of the breed structure were determined, and the influence of factory improver breeds on the microevolution of local breeds was estimated. the results of the research are correlated with the freely available results of the genome-wide analysis of foreign horse populations and breeds. The ways of identifying complexes that characterize the severity of selected traits in breeds with different directions of use are outlined. The high efficiency of using the obtained results to determine belonging to a particular breed is determined. A database of mitochondrial DNA and Y-chromosome haplotypes was created to conduct a retrospective analysis of the origin and refinement of breeding records in the system of centralized breeding records in horse breeding. As additional markers, we used targeted SNPs that characterize the severity of selected traits","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"174 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78014814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-252-254
М.Y. Rubtsova, N. Fursova, G. Presnova, M. Ulyashova, V. Grigorenko, А. Egorov
A method of hybridization analysis on colorimetric biochips for the quantitative determination of nucleic acids has been developed. The assay conditions and the method of processing digital images of biochips using different color models have been optimized. The method was applied for the quantitative determination of DNA and mRNA of serine beta-lactamases in Gram-negative bacteria resistant to beta-lactam antibiotics.
{"title":"HYBRIDIZATION ANALYSIS ON COLORIMETRIC BIOCHIPS FOR QUANTITATIVE DETERMINATION OF BETA-LACTAMASE GENES IN ANTIBIOTIC RESISTANT BACTERIA","authors":"М.Y. Rubtsova, N. Fursova, G. Presnova, M. Ulyashova, V. Grigorenko, А. Egorov","doi":"10.37747/2312-640x-2021-19-252-254","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-252-254","url":null,"abstract":"A method of hybridization analysis on colorimetric biochips for the quantitative determination of nucleic acids has been developed. The assay conditions and the method of processing digital images of biochips using different color models have been optimized. The method was applied for the quantitative determination of DNA and mRNA of serine beta-lactamases in Gram-negative bacteria resistant to beta-lactam antibiotics.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74941127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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