Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-280-282
D.S. Dzhulay, E.R. Mitina, A. Pshenichnikova
The conditions for cocultivation of aerobic methylotrophic bacteria Methylophilus quaylei and Methylorubrum extorquens and control of the content of individual strains in the consortium were optimized. It was shown that the production of exopolysaccharide and poly-3-hydroxybutyrate in a mixed culture is close to their production by pure cultures of M. quaylei and M. extorquens, respectively.
{"title":"MIXED CULTURE OF METHYLOTROPHIC BACTERIA METHYLOPHILUS QUAYLEI AND METHYLORUBRUM EXTORQUENS PRODUCES POLY-3-HYDROXYBUTYRATE AND EXOPOLYSACCHARIDE","authors":"D.S. Dzhulay, E.R. Mitina, A. Pshenichnikova","doi":"10.37747/2312-640x-2021-19-280-282","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-280-282","url":null,"abstract":"The conditions for cocultivation of aerobic methylotrophic bacteria Methylophilus quaylei and Methylorubrum extorquens and control of the content of individual strains in the consortium were optimized. It was shown that the production of exopolysaccharide and poly-3-hydroxybutyrate in a mixed culture is close to their production by pure cultures of M. quaylei and M. extorquens, respectively.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85957311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-329-330
V. B. Tsugkieva, A. Khoziev, L. B. Dzantieva, I. Soldatova
It has been found that the cup plant green mass is a promising substrate to culture yeasts. This plant in the Republic of North Ossetia-Alania is a perennial, high-yield introduced species, yielding up to 1500 cwt of green mass per hectare. Using the cup plant green mass to culture yeasts is economically preferable, due to the high nutrient content.
{"title":"TECHNOLOGY TO CULTURE YEASTS IN CULTURE MEDIA OF CUP PLANT GREEN MASS","authors":"V. B. Tsugkieva, A. Khoziev, L. B. Dzantieva, I. Soldatova","doi":"10.37747/2312-640x-2021-19-329-330","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-329-330","url":null,"abstract":"It has been found that the cup plant green mass is a promising substrate to culture yeasts. This plant in the Republic of North Ossetia-Alania is a perennial, high-yield introduced species, yielding up to 1500 cwt of green mass per hectare. Using the cup plant green mass to culture yeasts is economically preferable, due to the high nutrient content.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86137032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-271-273
L. Perminova, M. Pykhtina, A. Beklemishev
Biocatalytic processes for the synthesis of valuable products, such as different esters for various purposes, have been studied using heterogeneous biocatalysts prepared by immobilizing the recombinant lipase rPichia/lip on mesoporous silica (SiO2) and macroporous carbon aerogel (MCA). It was found that the functional properties of immobilized lipase, such as enzymatic activity, specificity toward pair of substrates (acid and alcohol), the molecules of which differ in the number of carbon atoms (C), as well as operational stability, depended on the method of adsorptive immobilization, the chemical nature of the support, and polarity of the organic solvent, i.e. logP. The functional properties of rPichia/lip have been shown to be modulated by the selection of an organic solvent and support for lipase immobilization.
{"title":"BIOCATALYTIC PROCESSES OF LOW-TEMPERATURE SYNTHESIS OF ESTERS. MODULATION OF THE FUNCTIONAL PROPERTIES OF LIPASE BY THE CHOICE OF SOLVENT AND SUPPORT FOR IMMOBILIZATION","authors":"L. Perminova, M. Pykhtina, A. Beklemishev","doi":"10.37747/2312-640x-2021-19-271-273","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-271-273","url":null,"abstract":"Biocatalytic processes for the synthesis of valuable products, such as different esters for various purposes, have been studied using heterogeneous biocatalysts prepared by immobilizing the recombinant lipase rPichia/lip on mesoporous silica (SiO2) and macroporous carbon aerogel (MCA). It was found that the functional properties of immobilized lipase, such as enzymatic activity, specificity toward pair of substrates (acid and alcohol), the molecules of which differ in the number of carbon atoms (C), as well as operational stability, depended on the method of adsorptive immobilization, the chemical nature of the support, and polarity of the organic solvent, i.e. logP. The functional properties of rPichia/lip have been shown to be modulated by the selection of an organic solvent and support for lipase immobilization.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89713064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-291-292
D. Ivasenko, E. V. Plotnikov, Y. Frank
Fungal isolates were obtained from the dumps of a wood processing enterprise. Their physiology was studied, and partial species identification was carried out in the ITS region.
从某木材加工企业的垃圾场中分离出真菌。对其生理特征进行了研究，并在ITS区进行了部分物种鉴定。
{"title":"ISOLATION OF MICROMYCETES, POTENTIAL LIGNIN DESTRUCTORS, FROM WOOD PROCESSING ENTERPRISES WASTE","authors":"D. Ivasenko, E. V. Plotnikov, Y. Frank","doi":"10.37747/2312-640x-2021-19-291-292","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-291-292","url":null,"abstract":"Fungal isolates were obtained from the dumps of a wood processing enterprise. Their physiology was studied, and partial species identification was carried out in the ITS region.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83635501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-349-352
A. M. Aibazov, T. Mamontova, V. Kharzinova, N. Zinovieva
Data have been obtained indicating differences by the live weight and exterior appearance of Karachaev goats bred in the mountain area, foothill, and plain-hill zone. A high level of genetic diversity and a clear genetic differentiation of populations from different breeding zones on the basis of STR-markers have been established.
{"title":"GENETIC DIFFERENTIATION BY STR-MARKERS AND PRODUCTIVITY TRAITS OF KARACHAEV GOATS FROM DIFFERENT BREEDING ZONES","authors":"A. M. Aibazov, T. Mamontova, V. Kharzinova, N. Zinovieva","doi":"10.37747/2312-640x-2021-19-349-352","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-349-352","url":null,"abstract":"Data have been obtained indicating differences by the live weight and exterior appearance of Karachaev goats bred in the mountain area, foothill, and plain-hill zone. A high level of genetic diversity and a clear genetic differentiation of populations from different breeding zones on the basis of STR-markers have been established.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75697902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-373-374
G. Demidenko
The development of research skills among undergraduates is one of the significant elements of the educational process, which allows forming the competencies laid down by the federal state educational standards of higher education and preparing highly qualified research personnel.
{"title":"THE IMPORTANCE OF THE DISCIPLINES OF BLOCK 1 FOR THE DEVELOPMENT OF RESEARCH SKILLS OF UNDERGRADUATES STUDYING IN THE DIRECTION OF BIOTECHNOLOGY","authors":"G. Demidenko","doi":"10.37747/2312-640x-2021-19-373-374","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-373-374","url":null,"abstract":"The development of research skills among undergraduates is one of the significant elements of the educational process, which allows forming the competencies laid down by the federal state educational standards of higher education and preparing highly qualified research personnel.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"372 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75738626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-75-77
O. Stepanova, E. Karsuntseva, G. Fursa, A. Chadin, M. Valikhov, A.P Semkina, I. Reshetov, V. Chekhonin
Enriched cultures of olfactory ensheathing cells and neural stem/progenitor cells were obtained according to our developed protocols from the olfactory mucosa of rat and human. It has been shown that only transplantation of human and rat olfactory ensheathing cells leads to a significant decrease in the size of cysts, as well as their complete disappearance in some animals.
{"title":"OBTAINING OF CELLULAR PREPARATIONS OF RAT AND HUMAN OLFACTORY MUCOSA AND THEIR INFLUENCE ON THE SIZE OF MODELED SPINAL CORD CYSTALS","authors":"O. Stepanova, E. Karsuntseva, G. Fursa, A. Chadin, M. Valikhov, A.P Semkina, I. Reshetov, V. Chekhonin","doi":"10.37747/2312-640x-2021-19-75-77","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-75-77","url":null,"abstract":"Enriched cultures of olfactory ensheathing cells and neural stem/progenitor cells were obtained according to our developed protocols from the olfactory mucosa of rat and human. It has been shown that only transplantation of human and rat olfactory ensheathing cells leads to a significant decrease in the size of cysts, as well as their complete disappearance in some animals.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76058874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-266-267
V. Agapova, S. Alferov
Comparative characteristics of the of BFC based on the membrane fraction of bacteria Gluconobacter oxydans used in the form of a suspension, as well as immobilized in PVA modified with N-vinylpyrrolidone ware carried out.
{"title":"COMPARATIVE CHARACTERISTICS OF BIOFUEL ELEMENTS BASED ON DIFFERENT BIOCATALYSTS","authors":"V. Agapova, S. Alferov","doi":"10.37747/2312-640x-2021-19-266-267","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-266-267","url":null,"abstract":"Comparative characteristics of the of BFC based on the membrane fraction of bacteria Gluconobacter oxydans used in the form of a suspension, as well as immobilized in PVA modified with N-vinylpyrrolidone ware carried out.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77734722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-166-169
A. Kudryavtsev, N. N. Kryuchkov, A. A. Isaev
A candidate vaccine containing a corpuscular adjuvant and a recombinant glycosylated RBD domain of the SARS- CoV-2 virus has successfully passed preclinical studies and is approved for clinical trials. In the course of preclinical studies, the safety and high immunogenicity of the developed drug was shown in experiments on animals, including primates.
{"title":"DEVELOPMENT AND PRE-CLINICAL STUDIES OF THE RECOMBINANT VACCINE \"BETUVAX-COV-2\" FOR PREVENTION OF COVID-19","authors":"A. Kudryavtsev, N. N. Kryuchkov, A. A. Isaev","doi":"10.37747/2312-640x-2021-19-166-169","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-166-169","url":null,"abstract":"A candidate vaccine containing a corpuscular adjuvant and a recombinant glycosylated RBD domain of the SARS- CoV-2 virus has successfully passed preclinical studies and is approved for clinical trials. In the course of preclinical studies, the safety and high immunogenicity of the developed drug was shown in experiments on animals, including primates.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78241304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-121-122
N. Semenova, A. Manzhurtsev, A. Yakovlev, T. Akhadov, S. Varfolomeev
It was found that repeated excitation and the use of aspirin as an inhibitor of prostaglandin synthesis did not affect level of BOLD signal in visual cortex. It was found a decrease in hemodynamic response to visual stimulation in thalamus under the influence of aspirin.
{"title":"THE EFFECT OF ACETYLSALICYLIC ACID ON INTENSTITY OF BOLD SIGNAL IN HUMAN VISUAL CORTEX DURING VIDEO STIMULATION. FMRI STUDY","authors":"N. Semenova, A. Manzhurtsev, A. Yakovlev, T. Akhadov, S. Varfolomeev","doi":"10.37747/2312-640x-2021-19-121-122","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-121-122","url":null,"abstract":"It was found that repeated excitation and the use of aspirin as an inhibitor of prostaglandin synthesis did not affect level of BOLD signal in visual cortex. It was found a decrease in hemodynamic response to visual stimulation in thalamus under the influence of aspirin.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73215026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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