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MIXED CULTURE OF METHYLOTROPHIC BACTERIA METHYLOPHILUS QUAYLEI AND METHYLORUBRUM EXTORQUENS PRODUCES POLY-3-HYDROXYBUTYRATE AND EXOPOLYSACCHARIDE 甲基营养细菌(methylophilus quaylei)和methylorubrum extorquens混合培养可产生聚3-羟基丁酸酯和外多糖
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-280-282
D.S. Dzhulay, E.R. Mitina, A. Pshenichnikova
The conditions for cocultivation of aerobic methylotrophic bacteria Methylophilus quaylei and Methylorubrum extorquens and control of the content of individual strains in the consortium were optimized. It was shown that the production of exopolysaccharide and poly-3-hydroxybutyrate in a mixed culture is close to their production by pure cultures of M. quaylei and M. extorquens, respectively.
优化了好氧甲基营养细菌(Methylophilus quaylei)和Methylorubrum extorquens的共培养条件和菌株含量的控制。结果表明,在混合培养中,胞外多糖和聚3-羟基丁酸酯的产量分别接近于纯培养中和纯培养中的产量。
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引用次数: 0
TECHNOLOGY TO CULTURE YEASTS IN CULTURE MEDIA OF CUP PLANT GREEN MASS 杯状植物绿块培养基中酵母培养技术
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-329-330
V. B. Tsugkieva, A. Khoziev, L. B. Dzantieva, I. Soldatova
It has been found that the cup plant green mass is a promising substrate to culture yeasts. This plant in the Republic of North Ossetia-Alania is a perennial, high-yield introduced species, yielding up to 1500 cwt of green mass per hectare. Using the cup plant green mass to culture yeasts is economically preferable, due to the high nutrient content.
研究发现,杯状植物绿块是一种很有前途的酵母培养基质。该植物位于北奥塞梯-阿拉尼亚共和国,是一种多年生高产引种植物,每公顷产量可达1500英吨。利用杯状植物绿块培养酵母是经济上较好的,因为它的营养含量高。
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引用次数: 0
BIOCATALYTIC PROCESSES OF LOW-TEMPERATURE SYNTHESIS OF ESTERS. MODULATION OF THE FUNCTIONAL PROPERTIES OF LIPASE BY THE CHOICE OF SOLVENT AND SUPPORT FOR IMMOBILIZATION 低温合成酯的生物催化过程。溶剂和固定化载体的选择对脂肪酶功能特性的影响
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-271-273
L. Perminova, M. Pykhtina, A. Beklemishev
Biocatalytic processes for the synthesis of valuable products, such as different esters for various purposes, have been studied using heterogeneous biocatalysts prepared by immobilizing the recombinant lipase rPichia/lip on mesoporous silica (SiO2) and macroporous carbon aerogel (MCA). It was found that the functional properties of immobilized lipase, such as enzymatic activity, specificity toward pair of substrates (acid and alcohol), the molecules of which differ in the number of carbon atoms (C), as well as operational stability, depended on the method of adsorptive immobilization, the chemical nature of the support, and polarity of the organic solvent, i.e. logP. The functional properties of rPichia/lip have been shown to be modulated by the selection of an organic solvent and support for lipase immobilization.
以重组脂酶为载体,在介孔二氧化硅(SiO2)和大孔碳气凝胶(MCA)上固定化制备了非均相生物催化剂,研究了生物催化合成不同用途酯类等有价值产物的过程。结果表明,固定化脂肪酶的酶活性、对碳原子数(C)不同的底物(酸和醇)的特异性以及操作稳定性等功能特性取决于吸附固定化方法、载体的化学性质和有机溶剂的极性(logP)。脂肪酶固定化的有机溶剂和载体的选择可以调节酵母/唇的功能特性。
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引用次数: 0
ISOLATION OF MICROMYCETES, POTENTIAL LIGNIN DESTRUCTORS, FROM WOOD PROCESSING ENTERPRISES WASTE 从木材加工企业废弃物中分离出潜在木质素破坏者微菌
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-291-292
D. Ivasenko, E. V. Plotnikov, Y. Frank
Fungal isolates were obtained from the dumps of a wood processing enterprise. Their physiology was studied, and partial species identification was carried out in the ITS region.
从某木材加工企业的垃圾场中分离出真菌。对其生理特征进行了研究,并在ITS区进行了部分物种鉴定。
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引用次数: 0
GENETIC DIFFERENTIATION BY STR-MARKERS AND PRODUCTIVITY TRAITS OF KARACHAEV GOATS FROM DIFFERENT BREEDING ZONES 不同育种区卡拉恰耶夫山羊的遗传分化及生产性状
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-349-352
A. M. Aibazov, T. Mamontova, V. Kharzinova, N. Zinovieva
Data have been obtained indicating differences by the live weight and exterior appearance of Karachaev goats bred in the mountain area, foothill, and plain-hill zone. A high level of genetic diversity and a clear genetic differentiation of populations from different breeding zones on the basis of STR-markers have been established.
已获得的数据表明,在山区、丘陵和平原丘陵地区饲养的卡拉恰耶夫山羊的活重和外观存在差异。在str标记的基础上,建立了高水平的遗传多样性和不同繁殖区群体的明显遗传分化。
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引用次数: 0
THE IMPORTANCE OF THE DISCIPLINES OF BLOCK 1 FOR THE DEVELOPMENT OF RESEARCH SKILLS OF UNDERGRADUATES STUDYING IN THE DIRECTION OF BIOTECHNOLOGY 1块学科对生物技术方向本科生研究技能发展的重要性
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-373-374
G. Demidenko
The development of research skills among undergraduates is one of the significant elements of the educational process, which allows forming the competencies laid down by the federal state educational standards of higher education and preparing highly qualified research personnel.
大学生研究技能的发展是教育过程的重要组成部分之一,它可以形成联邦国家高等教育教育标准所规定的能力,并培养高素质的研究人员。
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引用次数: 0
THE EFFECT OF ACETYLSALICYLIC ACID ON INTENSTITY OF BOLD SIGNAL IN HUMAN VISUAL CORTEX DURING VIDEO STIMULATION. FMRI STUDY 乙酰水杨酸对视像刺激下人视皮层粗信号强度的影响。功能磁共振成像研究
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-121-122
N. Semenova, A. Manzhurtsev, A. Yakovlev, T. Akhadov, S. Varfolomeev
It was found that repeated excitation and the use of aspirin as an inhibitor of prostaglandin synthesis did not affect level of BOLD signal in visual cortex. It was found a decrease in hemodynamic response to visual stimulation in thalamus under the influence of aspirin.
结果发现,反复兴奋和阿司匹林作为前列腺素合成抑制剂对视觉皮层BOLD信号水平无影响。研究发现,在阿司匹林的影响下,丘脑对视觉刺激的血流动力学反应降低。
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引用次数: 0
CHANGES IN PH AND CONCENTRATIONS OF METABOLITES IN THE HUMAN BRAIN UNDER THE INFLUENCE OF MILD TRAUMATIC BRAIN INJURY ACCORDING TO LOCALIZATION PROTON MAGNETIC RESONANCE SPECTROSCOPY 基于定位质子磁共振波谱的轻度创伤性脑损伤对人脑ph和代谢物浓度的影响
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-111-112
P. Bulanov, N. Semenova, T. Akhadov, A. Manzhurtsev, A. Yakovlev, M. V. Ublinsky, P. Menshchikov
Magnetic resonance spectroscopy (MRS) is the only method that allows in vivo study of biochemical processes in norm and pathology. The aim of the study was to identify the effect of mild traumatic brain injury (mTBI) on the concentrations of the most important cerebral metabolites and pH values in the human brain according to 1H MRC data.
磁共振波谱(MRS)是唯一一种可以在体内研究正常和病理生物化学过程的方法。本研究的目的是根据1H MRC数据确定轻度创伤性脑损伤(mTBI)对人脑中最重要的脑代谢物浓度和pH值的影响。
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引用次数: 0
METABOLIC PROFILE AND QUALITATIVE TRAITS OF MILK OF KARACHAEV AND NUBIAN GOATS 卡拉恰耶夫山羊和努比亚山羊乳汁代谢特征及质量性状
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-354-357
M. Selionova, A. M. Aibazov, N. Bogolyubova, N. Zinovieva
More intensive processes of protein and carbohydrate-lipid metabolism were revealed in Karachaev goats. With an increase in the number of lactation in the blood of Nubian goats, the level of protein, glucose and cholesterol increased, and Karachaev goats – urea and triglycerides. The milk of both breeds was characterized by a high content of fat, protein, casein, with a greater amount of them in the milk of Karachaev goats.
卡拉恰耶夫山羊的蛋白质和糖脂代谢过程更为剧烈。随着努比亚山羊血液中泌乳次数的增加,蛋白质、葡萄糖和胆固醇水平升高,卡拉恰耶夫山羊-尿素和甘油三酯水平升高。这两个品种的奶的特点是脂肪、蛋白质和酪蛋白含量高,卡拉恰耶夫山羊的奶中脂肪、蛋白质和酪蛋白含量更高。
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引用次数: 0
INCLUSION OF BIOCATALYSTS INTO THE PROCESS OF CHEMICAL- BIOLOGICAL OXIDATIVE DESULFURIZATION OF OIL FRACTIONS 生物催化剂在油分化学-生物氧化脱硫过程中的应用
Pub Date : 2021-01-01 DOI: 10.37747/2312-640x-2021-19-277-279
O. Senko, Stepanov N.A., М. Gladchenko, S. Gaydamaka, A. Akopyan, P. Polikarpova, Е. Eseva, S. Lysenko, А. Anisimov, E. Efremenko
A number of approaches are proposed, combined and tested to ensure the most effective introduction of biocatalysts into the process of chemical and biocatalytic desulfurization of liquid hydrocarbon raw materials: selection and use of environmentally friendly reagents, formation of immobilized artificial consortia, their preliminary adaptation to media, pH control of the medium, introduction of co-substrates in the form of agricultural waste, etc.
为了确保将生物催化剂最有效地引入液态烃原料的化学和生物催化脱硫过程,提出了多种方法,并进行了组合和测试:选择和使用环境友好型试剂,形成固定化人工联合体,对培养基的初步适应,培养基的pH控制,以农业废物的形式引入共底物等。
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引用次数: 0
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