S. Khelfi, S. Zerizer, Souraya Tebibel, C. Bensouici, Z. Kabouche
Abstract Hyperhomocysteinemia is considered to be one of the risk factors for inflammatory bowel disease (IBD), it is a chronic, relapsing, and remittent inflammatory disease of the gastrointestinal tract. Citrus sinensis L. has been used traditionally to treat bowel disorders. The present study aims to quantify the phenolic and flavonoid contents of the Citrus sinensis L. fruit (ORF) extract and to evaluate in vitro the antioxidant activity and the anti-inflammatory effect of ORF in vivo on intestinal inflammation induced by hyperhomocysteinemia. The total phenolic and flavonoid contents of the extract were determinated using the spectrophotometric method and the evaluation of antioxidant activity was performed by four methods: DPPH, ABTS, CUPRAC, and reducing power. The inflammatory marker (plasma homocysteine), the reduced glutathione (GSH) content in the liver tissue were measured and the histological sections of the intestines of the mice used were examined to assess the anti-inflammatory activity of ORF. The results of this study showed that the ethanolic extract of ORF possessed high phenolic content and exhibited good antioxidant activity. The use of ORF powder in the in vivo study showed an increase in GSH levels, a decrease in plasma homocysteine levels, and a restoration of the integrity of the intestinal epithelium. Keywords: Hyperhomocysteinemia, Intestinal Inflammation, Citrus sinensis L. fruit, Antioxidant activity, Anti-inflammatory
{"title":"The Antioxidant Activity and the Anti-inflammatory Effect of Citrus sinensis L. Fruit on Intestinal Inflammation Induced by Hyperhomocysteinemia in Mice","authors":"S. Khelfi, S. Zerizer, Souraya Tebibel, C. Bensouici, Z. Kabouche","doi":"10.12982/nlsc.2023.009","DOIUrl":"https://doi.org/10.12982/nlsc.2023.009","url":null,"abstract":"Abstract Hyperhomocysteinemia is considered to be one of the risk factors for inflammatory bowel disease (IBD), it is a chronic, relapsing, and remittent inflammatory disease of the gastrointestinal tract. Citrus sinensis L. has been used traditionally to treat bowel disorders. The present study aims to quantify the phenolic and flavonoid contents of the Citrus sinensis L. fruit (ORF) extract and to evaluate in vitro the antioxidant activity and the anti-inflammatory effect of ORF in vivo on intestinal inflammation induced by hyperhomocysteinemia. The total phenolic and flavonoid contents of the extract were determinated using the spectrophotometric method and the evaluation of antioxidant activity was performed by four methods: DPPH, ABTS, CUPRAC, and reducing power. The inflammatory marker (plasma homocysteine), the reduced glutathione (GSH) content in the liver tissue were measured and the histological sections of the intestines of the mice used were examined to assess the anti-inflammatory activity of ORF. The results of this study showed that the ethanolic extract of ORF possessed high phenolic content and exhibited good antioxidant activity. The use of ORF powder in the in vivo study showed an increase in GSH levels, a decrease in plasma homocysteine levels, and a restoration of the integrity of the intestinal epithelium. Keywords: Hyperhomocysteinemia, Intestinal Inflammation, Citrus sinensis L. fruit, Antioxidant activity, Anti-inflammatory","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114537921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Maslikah, S. Lestari, N. Handayani, W. E. Putra, Alif Rofiqotun Nurul Alimah, A. Amalia, S. Afifah, Siti Nur Arifah
Abstract Rheumatoid arthritis (RA) is a systemic autoimmune disease that causes inflammation of the synovial tissue bone as well as joint damage. Furthermore, an increase in the level of pro-inflammatory cytokines, such as tumor necrosis factor α (TNF-α) due to overexpression in the nuclear factor-kappa B (NFκB) contributes to the progression of the disease. NFκB also plays an important role in the production, differentiation, and effector function of inflammatory T cells. Red betel (Piper crocatum) leaf (RBL) is an Indonesian herb, which contains bioactive compounds such as flavonoids, terpenoids, and phenolic compounds. It is widely used as an intervention for various diseases including inflammatory-related diseases. Therefore, this study aims to evaluate the therapeutic effect of RBL extract as an anti-inflammatory agent through inhibition on the TNF receptor 1(TNFR1), NFκB, and inhibitor kappa B kinase (IκK) by molecular docking study. Oral toxicity prediction was carried out before molecular docking. Molecular docking performed using PyRx 0.8 software. The amino acid residues analysis and visualization were conducted using the Biovia Discovery Studio and Pymol. The toxicity prediction using ProTox-II showed that RBL active compounds are categorized between the 4th-6th class. Furthermore, the compounds, specifically kaempferitrin and apigenin have greater binding affinity compared to the drug inhibitor in NFκB signalling pathway. Based on the results, RBL active compounds can potentially act as an anti-inflammatory agent in RA, but further studies must be carried out to explore the potency of RBL through in vitro and in vivo effects. Keywords: Red betel leaf, Molecular docking, Rheumatoid arthritis, Flavonoid
{"title":"The Anti-Inflammatory Potential of Red Betel (Piper crocatum) Leaves Through Inhibitory Mechanism on Nfκb Signaling Pathway: Drug-Like Candidate Study","authors":"S. Maslikah, S. Lestari, N. Handayani, W. E. Putra, Alif Rofiqotun Nurul Alimah, A. Amalia, S. Afifah, Siti Nur Arifah","doi":"10.12982/nlsc.2023.005","DOIUrl":"https://doi.org/10.12982/nlsc.2023.005","url":null,"abstract":"Abstract Rheumatoid arthritis (RA) is a systemic autoimmune disease that causes inflammation of the synovial tissue bone as well as joint damage. Furthermore, an increase in the level of pro-inflammatory cytokines, such as tumor necrosis factor α (TNF-α) due to overexpression in the nuclear factor-kappa B (NFκB) contributes to the progression of the disease. NFκB also plays an important role in the production, differentiation, and effector function of inflammatory T cells. Red betel (Piper crocatum) leaf (RBL) is an Indonesian herb, which contains bioactive compounds such as flavonoids, terpenoids, and phenolic compounds. It is widely used as an intervention for various diseases including inflammatory-related diseases. Therefore, this study aims to evaluate the therapeutic effect of RBL extract as an anti-inflammatory agent through inhibition on the TNF receptor 1(TNFR1), NFκB, and inhibitor kappa B kinase (IκK) by molecular docking study. Oral toxicity prediction was carried out before molecular docking. Molecular docking performed using PyRx 0.8 software. The amino acid residues analysis and visualization were conducted using the Biovia Discovery Studio and Pymol. The toxicity prediction using ProTox-II showed that RBL active compounds are categorized between the 4th-6th class. Furthermore, the compounds, specifically kaempferitrin and apigenin have greater binding affinity compared to the drug inhibitor in NFκB signalling pathway. Based on the results, RBL active compounds can potentially act as an anti-inflammatory agent in RA, but further studies must be carried out to explore the potency of RBL through in vitro and in vivo effects. Keywords: Red betel leaf, Molecular docking, Rheumatoid arthritis, Flavonoid","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130372535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-21DOI: 10.21203/rs.3.rs-964920/v1
R. Sawangjit, P. Dilokthornsakul, P. Chiowchanwisawakit, W. Louthrenoo, M. Osiri, Jeeranun Sucheewasilp, Sawanya Nampuan, U. Permsuwan
Physical disability and severity of skin disease are important factors for evaluating healthy utility in psoriatic arthritis (PsA) patient. This study aims to estimate health utility and its related factors incorporating both rheumatologic and dermatologic factors in PsA. This cross-sectional study performed in PsA patients in Thailand. EQ-5D-5L, Health Assessment Questionnaire Disability Index (HAQ-DI), and Psoriasis Area and Severity Index (PASI) tools were used to collect health utility, physical disability, and severity of psoriatic skin lesions by face-to-face interviewed. Linear regression analysis was performed to relate health utility and its related factors. Of 84 patients enrolled, 49 (58.3%) were female, 67 (79.8%) had remission or low disease activity. Eighty-three patients (98.8%) received conventional synthetic disease-modifying antirheumatic drugs (csDMARDs) or biologic DMARDs (bDMARDs). The mean±SD overall utility was 0.87±0.15. The utility score of patients with low disease activity was significantly greater than those with moderate to severe disease activity (0.89±0.12 vs 0.72±0.19, p<0.001). The HAQ-DI (unstandardized β = -0.167, 95%CI; -0.218 to -0.116, p<0.001) and PASI (unstandardized β = -0.006, 95%CI; -0.009 to -0.003, p<0.001) were found to be significant related factors for utility. These results indicated that Thai patients with PsA had relatively high health utility. However, most included patients were in remission or had low disease activity. The HAQ-DI and PASI showed a strong predictors of patients’ health utility.
{"title":"Utility and Its Relationship With Disease Activity and Physical Disability of Patients With Psoriatic Arthritis in Thailand","authors":"R. Sawangjit, P. Dilokthornsakul, P. Chiowchanwisawakit, W. Louthrenoo, M. Osiri, Jeeranun Sucheewasilp, Sawanya Nampuan, U. Permsuwan","doi":"10.21203/rs.3.rs-964920/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-964920/v1","url":null,"abstract":"\u0000 Physical disability and severity of skin disease are important factors for evaluating healthy utility in psoriatic arthritis (PsA) patient. This study aims to estimate health utility and its related factors incorporating both rheumatologic and dermatologic factors in PsA. This cross-sectional study performed in PsA patients in Thailand. EQ-5D-5L, Health Assessment Questionnaire Disability Index (HAQ-DI), and Psoriasis Area and Severity Index (PASI) tools were used to collect health utility, physical disability, and severity of psoriatic skin lesions by face-to-face interviewed. Linear regression analysis was performed to relate health utility and its related factors. Of 84 patients enrolled, 49 (58.3%) were female, 67 (79.8%) had remission or low disease activity. Eighty-three patients (98.8%) received conventional synthetic disease-modifying antirheumatic drugs (csDMARDs) or biologic DMARDs (bDMARDs). The mean±SD overall utility was 0.87±0.15. The utility score of patients with low disease activity was significantly greater than those with moderate to severe disease activity (0.89±0.12 vs 0.72±0.19, p<0.001). The HAQ-DI (unstandardized β = -0.167, 95%CI; -0.218 to -0.116, p<0.001) and PASI (unstandardized β = -0.006, 95%CI; -0.009 to -0.003, p<0.001) were found to be significant related factors for utility. These results indicated that Thai patients with PsA had relatively high health utility. However, most included patients were in remission or had low disease activity. The HAQ-DI and PASI showed a strong predictors of patients’ health utility.","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130465376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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