Pimjai Doungsaard, S. Chansakaow, Worrapan Poomanee, J. Sirithunyalug, Nutjeera Intasai, P. Leelapornpisid
Abstract Many potential bioactive phytoconstituents in longan (Dimocarpus longan Lour.) leaf have been reported. Most chemical compounds belong to either phenolics or flavonoids, known as potent antioxidants. This research explores the multifunctional potential as a bioactive cosmetic ingredient used in skin care for adding value to longan leaves. The dried powder of longan leaves was extracted with 50%(v/v) ethanol in de-ionized water, then quantified for total phenolic and flavonoid contents. The hydroethanolic extract was investigated for various antioxidant methods including DPPH, nitric oxide (NO) scavenging and lipid peroxidation inhibition assays. The anti-tyrosinase activity was also evaluated for skin-whitening properties. Furthermore, Anti-aging activity in term of antiglycation was also conducted. The high number of polyphenols within the extract possessed a high antioxidant potential with IC50 0.023 ± 0.002, 0.478 ± 0.033 and 1.495 ± 0.153 mg/ml for DPPH scavenging, NO scavenging and lipid peroxidation inhibition, respectively. The extract could inhibit the tyrosinase enzyme with IC50 4.90 ± 0.50 and 6.60 ± 0.18 mg/ml for L-tyrosine and L-DOPA as substrates. The extract also exhibited a potent antiglycation activity with IC50 0.023 ± 0.003 mg/ml. Moreover, it showed no irritation effect on the chorioallantoic membranes (CAMs) within 5 min using the Hen’s Egg Test-Chorioallantoic Membranes (HET-CAMs). In conclusion, the polyphenolic-rich longan leaf extract displayed a good antioxidant, anti-tyrosinase and excellent antiglycation potential with safety that could constitute a promising multifunctional bioactive ingredient for skin care cosmeceutical products. Keywords: Longan leaf extract, Antioxidants, Anti-tyrosinase, Antiglycation, Irritation, HET-CAMs, Cosmeceutical
{"title":"Antioxidant, Anti-Tyrosinase, Antiglycation and Safety of Longan Leaf Extract for Cosmeceutical Application","authors":"Pimjai Doungsaard, S. Chansakaow, Worrapan Poomanee, J. Sirithunyalug, Nutjeera Intasai, P. Leelapornpisid","doi":"10.12982/nlsc.2023.052","DOIUrl":"https://doi.org/10.12982/nlsc.2023.052","url":null,"abstract":"Abstract Many potential bioactive phytoconstituents in longan (Dimocarpus longan Lour.) leaf have been reported. Most chemical compounds belong to either phenolics or flavonoids, known as potent antioxidants. This research explores the multifunctional potential as a bioactive cosmetic ingredient used in skin care for adding value to longan leaves. The dried powder of longan leaves was extracted with 50%(v/v) ethanol in de-ionized water, then quantified for total phenolic and flavonoid contents. The hydroethanolic extract was investigated for various antioxidant methods including DPPH, nitric oxide (NO) scavenging and lipid peroxidation inhibition assays. The anti-tyrosinase activity was also evaluated for skin-whitening properties. Furthermore, Anti-aging activity in term of antiglycation was also conducted. The high number of polyphenols within the extract possessed a high antioxidant potential with IC50 0.023 ± 0.002, 0.478 ± 0.033 and 1.495 ± 0.153 mg/ml for DPPH scavenging, NO scavenging and lipid peroxidation inhibition, respectively. The extract could inhibit the tyrosinase enzyme with IC50 4.90 ± 0.50 and 6.60 ± 0.18 mg/ml for L-tyrosine and L-DOPA as substrates. The extract also exhibited a potent antiglycation activity with IC50 0.023 ± 0.003 mg/ml. Moreover, it showed no irritation effect on the chorioallantoic membranes (CAMs) within 5 min using the Hen’s Egg Test-Chorioallantoic Membranes (HET-CAMs). In conclusion, the polyphenolic-rich longan leaf extract displayed a good antioxidant, anti-tyrosinase and excellent antiglycation potential with safety that could constitute a promising multifunctional bioactive ingredient for skin care cosmeceutical products. Keywords: Longan leaf extract, Antioxidants, Anti-tyrosinase, Antiglycation, Irritation, HET-CAMs, Cosmeceutical","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123889144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Narong Kamolrat, Settakorn Kamuang, Thiamthep Khamket, P. Sangmek, S. Sitthaphanit
Abstract Study for investigated the optimum photoperiod from blue LED light for the growth of Chlorella vulgaris in culture tank. Photoperiods from blue LED Light (Light/Dark) were set up at 24:0, 16:8 and 12:12 h. The initial number of C. vulgaris cells was 1.6 x 105 ± 0.12 cells mL-1. Culture medium was using commercially available fertilizers. After 17 days of cultivation, the results showed that the 24:0 h photoperiod had better growth of algae than 16:8 and 12:12 h, with maximum number of cells of 1.64 x 107 ± 0.23 cells mL-1 specific growth rate (SGR) was 0.17 ± 0.05, -0.06 ± 0.11 and -0.19 ± 0.1% day-1 with statistically significant differences (P < 0.05) in day 5 of cultivation. The stationary phase continued up to day 3 of cultivation, between the treatment. The optimum temperature for growth ranged from 35.5–38.5°C. The results showed that blue LED lighting at 24:0 h is the optimum photoperiod for cultivation of C. vulgaris. Keywords: Photobioreactor tank, Photoperiod, Blue LED light, Chlorella vulgaris, Growth rate
{"title":"The Effect of Optimum Photoperiod from Blue LED Light on Growth of Chlorella Vulgaris in Photobioreactor Tank","authors":"Narong Kamolrat, Settakorn Kamuang, Thiamthep Khamket, P. Sangmek, S. Sitthaphanit","doi":"10.12982/nlsc.2023.038","DOIUrl":"https://doi.org/10.12982/nlsc.2023.038","url":null,"abstract":"Abstract Study for investigated the optimum photoperiod from blue LED light for the growth of Chlorella vulgaris in culture tank. Photoperiods from blue LED Light (Light/Dark) were set up at 24:0, 16:8 and 12:12 h. The initial number of C. vulgaris cells was 1.6 x 105 ± 0.12 cells mL-1. Culture medium was using commercially available fertilizers. After 17 days of cultivation, the results showed that the 24:0 h photoperiod had better growth of algae than 16:8 and 12:12 h, with maximum number of cells of 1.64 x 107 ± 0.23 cells mL-1 specific growth rate (SGR) was 0.17 ± 0.05, -0.06 ± 0.11 and -0.19 ± 0.1% day-1 with statistically significant differences (P < 0.05) in day 5 of cultivation. The stationary phase continued up to day 3 of cultivation, between the treatment. The optimum temperature for growth ranged from 35.5–38.5°C. The results showed that blue LED lighting at 24:0 h is the optimum photoperiod for cultivation of C. vulgaris. Keywords: Photobioreactor tank, Photoperiod, Blue LED light, Chlorella vulgaris, Growth rate","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129453988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammadjavad Salehi, A. Tabesh, Amir Mohammad Mahdian
Abstract Pemphigus vulgaris (PV) is a chronic disease affecting oral mucosa. Oral health related quality of life (OHRQoL) is defined as a multidimensional construct that reflects comfort of people when eating, sleeping and engaging in social interaction, self-esteem, and satisfaction with respect to their oral health. The aim of this study was to measure OHRQoL and its correlation with disease severity of patients with PV. In this cross-sectional study, OHRQoL was measured by chronic oral mucosal disease questionnaire (COMDQ) in 72 patients (45.91 ± 11.45 years old) with PV include 45 (62.5%) women and 27 (37.5%). Finally, the obtained data were analyzed by statistical software. The mean total score of COMDQ in patients with PV was 72.41 ± 9.23. Moreover, mean daily dose of prednisolone corticosteroid was 0.619±0.395 mg/kg. COMDQ score in women was significantly higher than men (P<0.001). COMDQ total scores significantly correlated with daily dose of corticosteroid (P<0.001). Our study suggested that OHRQoL in patients with PV was not acceptable, and it was worse in the female gender that have more severe disease. Therefore, medical management of the underlying disease, as well as social and emotional care facilities seem necessary to improve OHRQoL in PV patients. Keywords: Pemphigus vulgaris, Oral health, Quality of life
{"title":"Does Pemphigus Vulgaris Affect Oral Health Related Quality of Life?","authors":"Mohammadjavad Salehi, A. Tabesh, Amir Mohammad Mahdian","doi":"10.12982/nlsc.2023.043","DOIUrl":"https://doi.org/10.12982/nlsc.2023.043","url":null,"abstract":"Abstract Pemphigus vulgaris (PV) is a chronic disease affecting oral mucosa. Oral health related quality of life (OHRQoL) is defined as a multidimensional construct that reflects comfort of people when eating, sleeping and engaging in social interaction, self-esteem, and satisfaction with respect to their oral health. The aim of this study was to measure OHRQoL and its correlation with disease severity of patients with PV. In this cross-sectional study, OHRQoL was measured by chronic oral mucosal disease questionnaire (COMDQ) in 72 patients (45.91 ± 11.45 years old) with PV include 45 (62.5%) women and 27 (37.5%). Finally, the obtained data were analyzed by statistical software. The mean total score of COMDQ in patients with PV was 72.41 ± 9.23. Moreover, mean daily dose of prednisolone corticosteroid was 0.619±0.395 mg/kg. COMDQ score in women was significantly higher than men (P<0.001). COMDQ total scores significantly correlated with daily dose of corticosteroid (P<0.001). Our study suggested that OHRQoL in patients with PV was not acceptable, and it was worse in the female gender that have more severe disease. Therefore, medical management of the underlying disease, as well as social and emotional care facilities seem necessary to improve OHRQoL in PV patients. Keywords: Pemphigus vulgaris, Oral health, Quality of life","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123768349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The aim of the study was to compare responses of different corn varieties to the fall armyworm Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) attack and to map patterns of single nucleotide polymorphism of the RGA genes. The study consisted of two activities, i.e., the field experiment to observe corn damage intensity and the laboratory observation to study the SNP pattern. Field experiment was conducted to compare eight corn varieties set in randomized block design with 3 replications. Damage intensity of the corn due to S. frugiperda attack was observed on the field and the corn samples were analyzed in the laboratory to examine the RGA (resistance gen analogue) which contained single nucleotide polymorphism (SNP). The results from the field and the laboratory analysis showed that the response of Bisi 321 variety to S. frugiperda attack was better than the other tested varieties. Based on the electrophoresis of the PCR results with the SNP2_MNBS_Alt primer, samples of corn varieties coded as V1 (Pertiwi 5), V3 (P-36), V6 (NK Super), V7 (Exotic), and V8 (Local) produced amplicons with the size of ±150 bp. The samples that did not have amplification were V2 (NK7328), V4 (Bisi 321), and V5 (Bisi 18). There is a possibility that the amplification results may indicate some degrees of tolerance clusters of corn varieties to S. frugiperda attack. Further validation is required to apply the SNP-based markers of corn to develop tolerant or resistant varieties. Keywords: Spodoptera frugiperda, RGA, SNP, Corn
{"title":"Evaluation of SNP-Based Markers Utilization for Resistance to Fall Armyworm Spodoptera frugiperda on Eight Corn Varieties","authors":"P. B. Timotiwu, A. ., W. Setiawan, H. Sudarsono","doi":"10.12982/nlsc.2023.039","DOIUrl":"https://doi.org/10.12982/nlsc.2023.039","url":null,"abstract":"Abstract The aim of the study was to compare responses of different corn varieties to the fall armyworm Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) attack and to map patterns of single nucleotide polymorphism of the RGA genes. The study consisted of two activities, i.e., the field experiment to observe corn damage intensity and the laboratory observation to study the SNP pattern. Field experiment was conducted to compare eight corn varieties set in randomized block design with 3 replications. Damage intensity of the corn due to S. frugiperda attack was observed on the field and the corn samples were analyzed in the laboratory to examine the RGA (resistance gen analogue) which contained single nucleotide polymorphism (SNP). The results from the field and the laboratory analysis showed that the response of Bisi 321 variety to S. frugiperda attack was better than the other tested varieties. Based on the electrophoresis of the PCR results with the SNP2_MNBS_Alt primer, samples of corn varieties coded as V1 (Pertiwi 5), V3 (P-36), V6 (NK Super), V7 (Exotic), and V8 (Local) produced amplicons with the size of ±150 bp. The samples that did not have amplification were V2 (NK7328), V4 (Bisi 321), and V5 (Bisi 18). There is a possibility that the amplification results may indicate some degrees of tolerance clusters of corn varieties to S. frugiperda attack. Further validation is required to apply the SNP-based markers of corn to develop tolerant or resistant varieties. Keywords: Spodoptera frugiperda, RGA, SNP, Corn","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125948175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Osteoarthritis (OA) is a progressive degradation of the cartilage that is strongly linked to the inflammation, and resulting in pain, stiffness, and loss of mobility. Currently, interventions aimed at reducing inflammation, alleviating pain, and enhancing joint mobility are recommend in patients with OA. Although intervention with traditional acupuncture has been shown to reduce pain, inflammation, and improve knee motion, the effects of acupuncture added Sanyinjiao (SP6) point on pain, inflammation and knee motion have never been investigated in patients with knee OA. Twenty-three patients with knee OA were subjected to receive acupuncture added SP6 twice a week for five weeks (totaling ten sessions). Patients were assessed at baseline and after ten sessions of acupuncture added SP6 using knee range of motion measurement, modified Western Ontario and McMaster Universities OA Index, numerical rating scale, and systemic inflammation levels. After the end of treatment, the knee pain was significantly reduced in patients when compared to baseline (P < 0.001). Addition to improvement in knee pain, knee flexion and extension also showed significant improved compared to baseline (P < 0.001). Furthermore, the low-grade systemic inflammation levels were decreased in patients when compared to baseline (P < 0.001). Therefore, this is the first study to show that acupuncture added SP6 point provides the beneficial effects on pain and inflammation, improving knee motion in patients with knee OA. Keywords: Acupuncture, Inflammation, Knee motion, Knee osteoarthritis, Pain, Chinese medicine
{"title":"Efficacy of Knee Acupuncture added Sanyinjiao Point for Patients with Knee Osteoarthritis Through Attenuated Systemic Inflammations","authors":"Warangkhana Klajing, Natticha Sumneang, Yin Shen Tan, Pongnared Jaengpromma, Anongporn Kobroob","doi":"10.12982/nlsc.2023.051","DOIUrl":"https://doi.org/10.12982/nlsc.2023.051","url":null,"abstract":"Abstract Osteoarthritis (OA) is a progressive degradation of the cartilage that is strongly linked to the inflammation, and resulting in pain, stiffness, and loss of mobility. Currently, interventions aimed at reducing inflammation, alleviating pain, and enhancing joint mobility are recommend in patients with OA. Although intervention with traditional acupuncture has been shown to reduce pain, inflammation, and improve knee motion, the effects of acupuncture added Sanyinjiao (SP6) point on pain, inflammation and knee motion have never been investigated in patients with knee OA. Twenty-three patients with knee OA were subjected to receive acupuncture added SP6 twice a week for five weeks (totaling ten sessions). Patients were assessed at baseline and after ten sessions of acupuncture added SP6 using knee range of motion measurement, modified Western Ontario and McMaster Universities OA Index, numerical rating scale, and systemic inflammation levels. After the end of treatment, the knee pain was significantly reduced in patients when compared to baseline (P < 0.001). Addition to improvement in knee pain, knee flexion and extension also showed significant improved compared to baseline (P < 0.001). Furthermore, the low-grade systemic inflammation levels were decreased in patients when compared to baseline (P < 0.001). Therefore, this is the first study to show that acupuncture added SP6 point provides the beneficial effects on pain and inflammation, improving knee motion in patients with knee OA. Keywords: Acupuncture, Inflammation, Knee motion, Knee osteoarthritis, Pain, Chinese medicine","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125062427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wariya Siriyod, P. Wanachantararak, T. Sastraruji, P. Chaijareenont, W. Chaiyana, S. Sookkhee, Darunee Owittayakul
Abstract The objectives of this study were to investigate the inhibitory effect of coconut oil denture-cleansing (CDC) formula against Candida albicans biofilm formed on 96-wells plate and acrylic surfaces at various immersion times and to determine the effects on physical properties of heat-cured acrylic surfaces. A total of twenty-seven CDC formulas were prepared. All CDC formulas were evaluated the stability and anticandidal activity by freeze-defrost cycles and broth microdilution assays, respectively. The formula 22th (F22) containing 0.3 g of anionic emulsifier at a ratio 40:60 of virgin coconut oil to distilled water showed the highest stability and anticandidal activity. The percent inhibition against C. albicans biofilm formed on 96-well plate and acrylic resin surfaces of the F22 after 8 hours of immersion were 86.87 ± 0.65%, and 91.19 ± 1.81%, respectively. However, the F22 had significantly lower inhibitory activity than 0.12% chlorhexidine gluconate (P <0.05). Furthermore, flexural strength of acrylic resin specimens was determined using a 3-point bending test and surface roughness was measured with a profilometer. The flexural strength and the change in surface roughness of F22 were not significant different compared with chlorhexidine and distilled water after 8 hours immersion for 30 days (P >0.05). In conclusions, F22 which containing 0.3 g of anionic emulsifier at the ratio of coconut oil to distilled water at 40:60 exhibited the potent inhibitory activity to Candida albicans biofilms and had no significant effect on the flexural strength and surface roughness of acrylic resins after immersion for 30 days. Keywords: Denture cleanser, Coconut oil, Anionic emulsifier, Biofilm, Candida albicans, Acrylic resins
{"title":"Novel Denture Cleanser Formulated From Virgin Coconut Oil and The Anionic Emulsifier Against Candida albicans Biofilms Formed on 96-Wells Plate and Acrylic Resin Surfaces","authors":"Wariya Siriyod, P. Wanachantararak, T. Sastraruji, P. Chaijareenont, W. Chaiyana, S. Sookkhee, Darunee Owittayakul","doi":"10.12982/nlsc.2023.047","DOIUrl":"https://doi.org/10.12982/nlsc.2023.047","url":null,"abstract":"Abstract The objectives of this study were to investigate the inhibitory effect of coconut oil denture-cleansing (CDC) formula against Candida albicans biofilm formed on 96-wells plate and acrylic surfaces at various immersion times and to determine the effects on physical properties of heat-cured acrylic surfaces. A total of twenty-seven CDC formulas were prepared. All CDC formulas were evaluated the stability and anticandidal activity by freeze-defrost cycles and broth microdilution assays, respectively. The formula 22th (F22) containing 0.3 g of anionic emulsifier at a ratio 40:60 of virgin coconut oil to distilled water showed the highest stability and anticandidal activity. The percent inhibition against C. albicans biofilm formed on 96-well plate and acrylic resin surfaces of the F22 after 8 hours of immersion were 86.87 ± 0.65%, and 91.19 ± 1.81%, respectively. However, the F22 had significantly lower inhibitory activity than 0.12% chlorhexidine gluconate (P <0.05). Furthermore, flexural strength of acrylic resin specimens was determined using a 3-point bending test and surface roughness was measured with a profilometer. The flexural strength and the change in surface roughness of F22 were not significant different compared with chlorhexidine and distilled water after 8 hours immersion for 30 days (P >0.05). In conclusions, F22 which containing 0.3 g of anionic emulsifier at the ratio of coconut oil to distilled water at 40:60 exhibited the potent inhibitory activity to Candida albicans biofilms and had no significant effect on the flexural strength and surface roughness of acrylic resins after immersion for 30 days. Keywords: Denture cleanser, Coconut oil, Anionic emulsifier, Biofilm, Candida albicans, Acrylic resins","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126774177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Anuchapreeda, Banphot Shaengkhamnang, Pawaret Panyajai, S. Chiampanichayakul, Singkome Tima, P. Klangsinsirikul, P. Vongchan, A. Tantiworawit
Abstract Hematopoietic stem cell transplantation (HSCT) is one method of lymphoma therapy. Peripheral blood stem cell (PBSC) separation, preservation, cell viability, and cell function before transplantation are important factors in the success rate of stem cell transplantation. This study aims to separate and estimate the efficiency of deep-freezing preservation in autologous PBSCs (CD34+/CD38-) from lymphoma patients undergoing stem cell transplantation. PBSCs were separated and collected by leukapheresis before being cryopreserved and stored in liquid nitrogen. The number of CD34+/CD45dim cells was investigated and compared with the subpopulation of CD34+/CD38- cells using conventional trypan blue exclusion method and 7-AAD before and after cryopreservation. Colony-forming units (CFUs) were determined to indirectly assess the viability and potency of the PBSCs. The result showed that CD34+/CD38– cells constituted 35.56% of total CD34+ cells and 0.05% of total nucleated cells (TNCs). After thawing, the number of CD34+/CD38- cells did not demonstrate significant differences compared with pre-storage. The percentage of CFU recovery was 94.74%. In this study, the storage process of deep-freezing cryopreservation demonstrated high-quality recovery of CD34+/CD38- cells from PBSCs for autologous hematopoietic stem cell transplantation in lymphoma patients. This result showed novel data about the preservation of CD34+/CD38- cells. Keywords: Lymphoma, Autologous peripheral blood stem cell, Blood cryopreservation, Bone marrow transplantation
{"title":"Preservation of Peripheral Blood Stem Cells (CD34+/CD38-) for Bone Marrow Transplantation in Thai Lymphoma Patients","authors":"S. Anuchapreeda, Banphot Shaengkhamnang, Pawaret Panyajai, S. Chiampanichayakul, Singkome Tima, P. Klangsinsirikul, P. Vongchan, A. Tantiworawit","doi":"10.12982/nlsc.2023.044","DOIUrl":"https://doi.org/10.12982/nlsc.2023.044","url":null,"abstract":"Abstract Hematopoietic stem cell transplantation (HSCT) is one method of lymphoma therapy. Peripheral blood stem cell (PBSC) separation, preservation, cell viability, and cell function before transplantation are important factors in the success rate of stem cell transplantation. This study aims to separate and estimate the efficiency of deep-freezing preservation in autologous PBSCs (CD34+/CD38-) from lymphoma patients undergoing stem cell transplantation. PBSCs were separated and collected by leukapheresis before being cryopreserved and stored in liquid nitrogen. The number of CD34+/CD45dim cells was investigated and compared with the subpopulation of CD34+/CD38- cells using conventional trypan blue exclusion method and 7-AAD before and after cryopreservation. Colony-forming units (CFUs) were determined to indirectly assess the viability and potency of the PBSCs. The result showed that CD34+/CD38– cells constituted 35.56% of total CD34+ cells and 0.05% of total nucleated cells (TNCs). After thawing, the number of CD34+/CD38- cells did not demonstrate significant differences compared with pre-storage. The percentage of CFU recovery was 94.74%. In this study, the storage process of deep-freezing cryopreservation demonstrated high-quality recovery of CD34+/CD38- cells from PBSCs for autologous hematopoietic stem cell transplantation in lymphoma patients. This result showed novel data about the preservation of CD34+/CD38- cells. Keywords: Lymphoma, Autologous peripheral blood stem cell, Blood cryopreservation, Bone marrow transplantation","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121021208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kamonlapob Boonrugsa, Kate Norkham, Siravit Chotimanon, Khetdan Panyadej, Rath Rerkasem, Pitchaya Singhavesjsakul, Ratchanee Somnabut, T. Sastraruji, S. Sookkhee
Abstract This study aimed to solve the diagnostic problem of AmpC enzyme production from ESBL production of community-acquired urinary tract-infected Escherichia coli from Maharaj Nakorn Chiang Mai Hospital in 2019-2020 by using the MALDI-TOF technique to search for the significant difference between the ceftazidime exposure alone and the concomitance of ESBL inhibitor, clavulanic acid, and AmpC inducer, cefoxitin. Among 254 E. coli isolates, 42.12% (n = 107) were categorized as high-ESBL producers after determined by E-strip test. The frequencies of six peaks, 2689 (88.19%), 3126 (90.55%), 6314 (91.73%), 6411 (88.98%), 7157 (90.55%), 10301 (85.09%) Da were suspected to be the E. coli specific peptide spectra. In the high-ESBL-producing group, three statistically significant peptide spectra which played an ESBL manner were only identified at 4613 (13.33%, P = 0.008), 5613 (11.67%, P = 0.018), and 9713 (55.00%, P < 0.001) Da. Three peptide spectra that acted in AmpC β-lactamase manner were statistically significant at 4184 (16.67%, P = 0.048), 9551 (10.00%, P = 0.021), and 10477 Da (38.33%, P = 0.006) in this group. Whereas the statistically significant peptide spectra which played as AmpC were identified at 5380 (80.00%, P = 0.005) and 6254 Da (66.67%, P = 0.016) in the non ESBL-producing group. Our results indicated that AmpC enzyme-related spectra could be detected in some high-ESBL and non ESBL-producing isolates after being induced by cefoxitin. Keywords: AmpC, MALDI-TOF, Cefoxitin, ESBL-producing E. coli, Drug resistance protein
摘要本研究旨在通过MALDI-TOF技术，解决2019-2020年清迈医院社区获得性尿路感染大肠埃希菌ESBL产AmpC酶的诊断问题，寻找头孢他啶单独暴露与ESBL抑制剂克拉维酸、AmpC诱导剂头孢西丁联合暴露的显著差异。经e条检测，254株大肠杆菌中有42.12% (n = 107)为高esbl产菌。6个峰的频率分别为2689(88.19%)、3126(90.55%)、6314(91.73%)、6411(88.98%)、7157(90.55%)、10301 (85.09%)Da，怀疑为大肠杆菌特异性肽谱。在高ESBL产生组中，三个具有统计学意义的肽谱只在4613 (13.33%，P = 0.008)、5613 (11.67%，P = 0.018)和9713 (55.00%，P < 0.001) Da被鉴定出来。3个以AmpC β-内酰胺酶方式作用的肽谱在该组分别为4184 (16.67%，P = 0.048)、9551 (10.00%，P = 0.021)和10477 Da (38.33%， P = 0.006)，差异均有统计学意义。而非产生esbl组的AmpC肽谱分别为5380 (80.00%，P = 0.005)和6254 Da (66.67%， P = 0.016)，具有统计学意义。结果表明，在头孢西丁诱导的一些高esbl和不产esbl的分离株中可以检测到AmpC酶相关光谱。关键词:AmpC, MALDI-TOF，头孢西丁，产esbl大肠杆菌，耐药蛋白
{"title":"Alteration of Drug-Resistant Proteins in ESBL-Producing Escherichia coli After Induction with Cefoxitin Using MALDI-TOF Technique","authors":"Kamonlapob Boonrugsa, Kate Norkham, Siravit Chotimanon, Khetdan Panyadej, Rath Rerkasem, Pitchaya Singhavesjsakul, Ratchanee Somnabut, T. Sastraruji, S. Sookkhee","doi":"10.12982/nlsc.2023.036","DOIUrl":"https://doi.org/10.12982/nlsc.2023.036","url":null,"abstract":"Abstract This study aimed to solve the diagnostic problem of AmpC enzyme production from ESBL production of community-acquired urinary tract-infected Escherichia coli from Maharaj Nakorn Chiang Mai Hospital in 2019-2020 by using the MALDI-TOF technique to search for the significant difference between the ceftazidime exposure alone and the concomitance of ESBL inhibitor, clavulanic acid, and AmpC inducer, cefoxitin. Among 254 E. coli isolates, 42.12% (n = 107) were categorized as high-ESBL producers after determined by E-strip test. The frequencies of six peaks, 2689 (88.19%), 3126 (90.55%), 6314 (91.73%), 6411 (88.98%), 7157 (90.55%), 10301 (85.09%) Da were suspected to be the E. coli specific peptide spectra. In the high-ESBL-producing group, three statistically significant peptide spectra which played an ESBL manner were only identified at 4613 (13.33%, P = 0.008), 5613 (11.67%, P = 0.018), and 9713 (55.00%, P < 0.001) Da. Three peptide spectra that acted in AmpC β-lactamase manner were statistically significant at 4184 (16.67%, P = 0.048), 9551 (10.00%, P = 0.021), and 10477 Da (38.33%, P = 0.006) in this group. Whereas the statistically significant peptide spectra which played as AmpC were identified at 5380 (80.00%, P = 0.005) and 6254 Da (66.67%, P = 0.016) in the non ESBL-producing group. Our results indicated that AmpC enzyme-related spectra could be detected in some high-ESBL and non ESBL-producing isolates after being induced by cefoxitin. Keywords: AmpC, MALDI-TOF, Cefoxitin, ESBL-producing E. coli, Drug resistance protein","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128090883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. D. Meiliana, P. S. Rejeki, M. Miftahussurur, Minidian Fasitasari
The prevalence of obesity is increasing worldwide over the years. One of the non-pharmacological therapy which is believed to be promote weight loss is ketogenic diet. Nevertheless, the long-term effects which may be caused by this diet are still debating, especially in the liver. This study aimed to determine the effect of long-term ketogenic diet (8 weeks) on serum alanine transaminase (ALT) levels. Twelve male mice were divided into basal diet (BD) and ketogenic diet (KD) group and given intervention for eight weeks adlibitum. Body weight was weighed in pre and post-intervention, while ALT levels were measured only once after eight weeks of intervention. As a result, pre-intervention body weight in the BD group was 25.170 ± 2.858 g and KD group was 27.170 ± 1.329 g (P =0.151). In the post-intervention, body weight in BD and KD group were 44.500 ± 5.244 g and 31.830 ± 5.707 g, respectively (P =0.003). BD group showed a significant difference between pre-and post-intervention body weight (P <0.0001); however, it was not significant in the KD group (P =0.096). After eight weeks, serum ALT levels in BD-fed mice were 90.672 ± 20.786 U/L and in KD-fed mice showed 117.037 ± 19.261 U/L (P =0.046). In conclusion, KD elevated serum ALT levels and attenuated weight gain after 8-week KD supplementation. Keywords: Alanine transaminase, Ketogenic diet, Liver, Mice, Obesity
近年来，肥胖症在世界范围内的患病率呈上升趋势。生酮饮食是一种被认为可以促进减肥的非药物疗法。然而，这种饮食可能造成的长期影响仍在争论中，尤其是对肝脏的影响。本研究旨在确定长期生酮饮食(8周)对血清丙氨酸转氨酶(ALT)水平的影响。将12只雄性小鼠分为基础饮食组(BD)和生酮饮食组(KD)，随机干预8周。在干预前和干预后测量体重，而ALT水平仅在干预八周后测量一次。结果，BD组干预前体重为25.170±2.858 g, KD组为27.170±1.329 g (P =0.151)。干预后BD组和KD组体重分别为44.500±5.244 g和31.830±5.707 g (P =0.003)。BD组干预前后体重差异有统计学意义(P <0.0001);而KD组差异无统计学意义(P =0.096)。8周后，bd饲喂小鼠血清ALT水平为90.672±20.786 U/L, kd饲喂小鼠血清ALT水平为117.037±19.261 U/L (P =0.046)。综上所述，补充8周KD后，KD提高了血清ALT水平，减轻了体重增加。关键词:丙氨酸转氨酶，生酮饮食，肝脏，小鼠，肥胖
{"title":"Effect of Long-term Ketogenic Diet on Serum Alanine Transaminase Levels in Mice (Mus musculus)","authors":"I. D. Meiliana, P. S. Rejeki, M. Miftahussurur, Minidian Fasitasari","doi":"10.12982/nlsc.2023.024","DOIUrl":"https://doi.org/10.12982/nlsc.2023.024","url":null,"abstract":"The prevalence of obesity is increasing worldwide over the years. One of the non-pharmacological therapy which is believed to be promote weight loss is ketogenic diet. Nevertheless, the long-term effects which may be caused by this diet are still debating, especially in the liver. This study aimed to determine the effect of long-term ketogenic diet (8 weeks) on serum alanine transaminase (ALT) levels. Twelve male mice were divided into basal diet (BD) and ketogenic diet (KD) group and given intervention for eight weeks adlibitum. Body weight was weighed in pre and post-intervention, while ALT levels were measured only once after eight weeks of intervention. As a result, pre-intervention body weight in the BD group was 25.170 ± 2.858 g and KD group was 27.170 ± 1.329 g (P =0.151). In the post-intervention, body weight in BD and KD group were 44.500 ± 5.244 g and 31.830 ± 5.707 g, respectively (P =0.003). BD group showed a significant difference between pre-and post-intervention body weight (P <0.0001); however, it was not significant in the KD group (P =0.096). After eight weeks, serum ALT levels in BD-fed mice were 90.672 ± 20.786 U/L and in KD-fed mice showed 117.037 ± 19.261 U/L (P =0.046). In conclusion, KD elevated serum ALT levels and attenuated weight gain after 8-week KD supplementation. Keywords: Alanine transaminase, Ketogenic diet, Liver, Mice, Obesity","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121282519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We examined recovery of bryophyte diversity during forest restoration by the framework species method (FSM), as a component of overall biodiversity recovery—one of the defining indicators of restoration success. The epiphytic bryophyte communities in FSM trial plots (13 and 21 years since tree planting), were compared with those of old-growth forest in the upper Mae Sa Valley, northern Thailand. Bryophytes were surveyed in June 2019, on the bark of 3 framework tree species: Archidendron clypearia, Erythrina subumbrans and Sarcosperma arboreum. Fifteen species were recorded, representing 14 genera and 12 families in 3 morphological groups: acrocarpous mosses 4 species (27%), pleurocarpous mosses 5 species (33%) and leafy liverworts 6 species (40%). Within 13 years after restoration initiation, bryophyte species richness had increased to the same level as that recorded in old-growth forest. Furthermore, the bryophyte community species composition of the older restoration forest was more similar to that of natural forest than was the bryophyte community species composition of younger forest. We conclude that the FSM effectively recovers bryophyte diversity and directs the successional trajectory of the bryophyte community towards that of old growth forest within an acceptable time span. The use of bryophytes as indicators of forest restoration progress is discussed. Keywords: Conservation, Framework species method, Liverwort, Moss, Species richness
{"title":"Effects of Forest Restoration Age on the Species Diversity of Epiphytic Bryophyte Communities","authors":"Puwadol Chawengkul, Pranee Nangngam, S. Elliott","doi":"10.12982/nlsc.2023.022","DOIUrl":"https://doi.org/10.12982/nlsc.2023.022","url":null,"abstract":"We examined recovery of bryophyte diversity during forest restoration by the framework species method (FSM), as a component of overall biodiversity recovery—one of the defining indicators of restoration success. The epiphytic bryophyte communities in FSM trial plots (13 and 21 years since tree planting), were compared with those of old-growth forest in the upper Mae Sa Valley, northern Thailand. Bryophytes were surveyed in June 2019, on the bark of 3 framework tree species: Archidendron clypearia, Erythrina subumbrans and Sarcosperma arboreum. Fifteen species were recorded, representing 14 genera and 12 families in 3 morphological groups: acrocarpous mosses 4 species (27%), pleurocarpous mosses 5 species (33%) and leafy liverworts 6 species (40%). Within 13 years after restoration initiation, bryophyte species richness had increased to the same level as that recorded in old-growth forest. Furthermore, the bryophyte community species composition of the older restoration forest was more similar to that of natural forest than was the bryophyte community species composition of younger forest. We conclude that the FSM effectively recovers bryophyte diversity and directs the successional trajectory of the bryophyte community towards that of old growth forest within an acceptable time span. The use of bryophytes as indicators of forest restoration progress is discussed. Keywords: Conservation, Framework species method, Liverwort, Moss, Species richness","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131659226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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