首页 > 最新文献

Indian Journal of Clinical Biochemistry最新文献

英文 中文
A Study of Maternal and Umbilical Cord Blood Lead Levels in Pregnant Women. 孕妇母体和脐带血铅含量研究。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-06-22 DOI: 10.1007/s12291-022-01040-0
Abbas Ali Mahdi, Jamal Akhtar Ansari, Priyanka Chaurasia, Mohammad Kaleem Ahmad, Shipra Kunwar, Sally McClean, Pratheepan Yogarajah

Lead is a highly toxic element which can cross the placental barrier and enter the fetus during pregnancy. Parental lead exposure has adverse effect on infant as well as on maternal health. As part of our program to investigate the lead poisoning in human population we investigated the maternal blood lead levels (MBLL) and umbilical cord blood lead (UBLL) levels in 200 pregnant women and collected their socio-demographic details. In the study we found high lead levels in both maternal and umbilical cord blood samples. The results showed 47.5% maternal blood (n = 95) detected with lead while 38.5% umbilical cord blood (n = 77) samples had lead concentration higher than that of reference range of ≤ 5 µg/dL. We also found that the Spearman's correlation coefficient (rs) revealed a strong positive correlation between the MBLL and UBLL (rs = 0.63). The results from socio-demographic questionnaire demonstrated that the recent home painting (p = 0.002) and residing close proximity to traffic congestion (p = 0.05) were significantly associated with MBLL. Education, mother age, fuel and water sources were not significantly associated with MBLL. Iron and calcium deficiency along with tiredness, lethargy, abdominal pain were also reported in women having high lead level > 5 µg/dL. Concludingly, on the basis of results obtained it may be stated that we found elevated BLLs in both pregnant women as well as in umbilical cord blood. The prevalence of elevated lead levels in mothers will expose the fetus to lead through placental barriers mobilization and it can have long term adverse effects on the developing fetus. Therefore, it is recommended that screening of blood lead levels be carried out in high-risk women based on their social, occupational, environmental, and individual factors. In addition, stringent regulations on lead-based products are also required from government agencies/authorities to reduce environmental lead burden and toxicity. Moreover, public awareness programs should be organized on hazardous effect of lead.

铅是一种剧毒元素,可在怀孕期间穿过胎盘屏障进入胎儿体内。父母接触铅会对婴儿和母亲的健康产生不良影响。作为调查人类铅中毒计划的一部分,我们调查了 200 名孕妇的母体血铅含量(MBL)和脐带血铅含量(UBLL),并收集了她们的社会人口学详细信息。在研究中,我们发现母体和脐带血样本中的铅含量都很高。结果显示,47.5% 的母血样本(95 人)检测到铅含量,而 38.5% 的脐带血样本(77 人)铅含量高于参考值范围(≤ 5 µg/dL)。我们还发现,斯皮尔曼相关系数(rs)显示 MBLL 和 UBLL 之间存在很强的正相关性(rs = 0.63)。社会人口学问卷调查结果显示,最近粉刷过的房屋(p = 0.002)和居住地靠近交通拥堵地区(p = 0.05)与 MBLL 显著相关。教育程度、母亲年龄、燃料和水源与母婴传播疾病无明显关系。据报告,铅含量大于 5 µg/dL 的妇女还会出现缺铁、缺钙、疲倦、嗜睡、腹痛等症状。总之,根据所获得的结果,我们发现孕妇和脐带血中的铅含量都升高了。母亲体内铅含量的普遍升高会使胎儿通过胎盘屏障接触到铅,并对发育中的胎儿产生长期的不良影响。因此,建议根据社会、职业、环境和个人因素对高危妇女进行血铅水平筛查。此外,政府机构/主管部门也需要对铅基产品进行严格监管,以减少环境中铅的负担和毒性。此外,还应组织有关铅有害影响的公众宣传计划。
{"title":"A Study of Maternal and Umbilical Cord Blood Lead Levels in Pregnant Women.","authors":"Abbas Ali Mahdi, Jamal Akhtar Ansari, Priyanka Chaurasia, Mohammad Kaleem Ahmad, Shipra Kunwar, Sally McClean, Pratheepan Yogarajah","doi":"10.1007/s12291-022-01040-0","DOIUrl":"10.1007/s12291-022-01040-0","url":null,"abstract":"<p><p>Lead is a highly toxic element which can cross the placental barrier and enter the fetus during pregnancy. Parental lead exposure has adverse effect on infant as well as on maternal health. As part of our program to investigate the lead poisoning in human population we investigated the maternal blood lead levels (MBLL) and umbilical cord blood lead (UBLL) levels in 200 pregnant women and collected their socio-demographic details. In the study we found high lead levels in both maternal and umbilical cord blood samples. The results showed 47.5% maternal blood (<i>n</i> = 95) detected with lead while 38.5% umbilical cord blood (<i>n</i> = 77) samples had lead concentration higher than that of reference range of ≤ 5 µg/dL. We also found that the Spearman's correlation coefficient (rs) revealed a strong positive correlation between the MBLL and UBLL (rs = 0.63). The results from socio-demographic questionnaire demonstrated that the recent home painting (<i>p</i> = 0.002) and residing close proximity to traffic congestion (<i>p</i> = 0.05) were significantly associated with MBLL. Education, mother age, fuel and water sources were not significantly associated with MBLL. Iron and calcium deficiency along with tiredness, lethargy, abdominal pain were also reported in women having high lead level > 5 µg/dL. Concludingly, on the basis of results obtained it may be stated that we found elevated BLLs in both pregnant women as well as in umbilical cord blood. The prevalence of elevated lead levels in mothers will expose the fetus to lead through placental barriers mobilization and it can have long term adverse effects on the developing fetus. Therefore, it is recommended that screening of blood lead levels be carried out in high-risk women based on their social, occupational, environmental, and individual factors. In addition, stringent regulations on lead-based products are also required from government agencies/authorities to reduce environmental lead burden and toxicity. Moreover, public awareness programs should be organized on hazardous effect of lead.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"94-101"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10585901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Magnesium Supplementation Affects the Expression of Sirtuin1, Tumor Protein P53 and Endothelial Nitric Oxide Synthase Genes in Patients with Atherosclerosis: A Double-Blind, Randomized, Placebo-Controlled Trial. 补充镁可影响动脉粥样硬化患者体内 Sirtuin1、肿瘤蛋白 P53 和内皮一氧化氮合成酶基因的表达:双盲、随机、安慰剂对照试验。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-04-09 DOI: 10.1007/s12291-022-01032-0
Behnaz Rahnama Inchehsablagh, Farhad Ghadiri Soufi, Shabnaz Koochakkhani, Fariba Azarkish, Hossein Farshidi, Mahdiye Eslami, Masoumeh Mahmoodi, Nepton Soltani, Ebrahim Eftekhar

Magnesium seems to play a role in improving cardiovascular function, but its exact mechanism is unknown. In this study, we hypothesized that magnesium could modulate the expression of genes involved in atherosclerosis. The aim of the present investigation was to evaluate the effect of magnesium sulfate on the expression of sirtuin1 (SIRT1), tumor protein p53 (TP53), and endothelial nitric oxide synthase (eNOS) genes in patients with atherosclerosis. This study was a placebo-controlled double-blind randomized clinical trial on 56 patients with angiographically proven atherosclerosis. Participants were randomly divided into two groups receiving 300 mg/day magnesium sulfate (n = 29) and placebo (n = 27) for three months (following up every month). Fasting blood samples were taken before and after the intervention and total RNA was extracted and used to evaluate the expression level of SIRT1, TP53, and eNOS genes by Real-Time PCR. The expression of eNOS gene was significantly increased (P < 0.0001) and the expression of TP53 gene was decreased (P = 0.02) in the magnesium sulfate group compared to the placebo group. But SIRT1 gene expression was not significantly different between the two groups. Our findings demonstrate that magnesium sulfate supplementation may have a protective role against the progression of atherosclerosis through upregulation of eNOS and downregulation of TP53 gene. Trial registration: This present clinical trial has been registered in the Iranian Registry of Clinical Trials (IRCT) with the registration code of "IRCT20151028024756N3", https://www.irct.ir/trial/29097?revision=114102. Registered on 16 December 2019.

镁似乎在改善心血管功能方面发挥作用,但其确切机制尚不清楚。在本研究中,我们假设镁能调节动脉粥样硬化相关基因的表达。本研究旨在评估硫酸镁对动脉粥样硬化患者体内 sirtuin1(SIRT1)、肿瘤蛋白 p53(TP53)和内皮一氧化氮合酶(eNOS)基因表达的影响。这项研究是一项安慰剂对照双盲随机临床试验,对象是 56 名经血管造影证实患有动脉粥样硬化的患者。参与者被随机分为两组,分别接受每天 300 毫克硫酸镁(29 人)和安慰剂(27 人),为期三个月(每月随访一次)。干预前后采集空腹血样,提取总 RNA,并通过实时 PCR 评估 SIRT1、TP53 和 eNOS 基因的表达水平。与安慰剂组相比,硫酸镁组 eNOS 基因的表达明显增加(P P = 0.02)。但 SIRT1 基因的表达在两组间无明显差异。我们的研究结果表明,补充硫酸镁可通过上调 eNOS 和下调 TP53 基因对动脉粥样硬化的进展起到保护作用。试验注册:本临床试验已在伊朗临床试验注册中心(IRCT)注册,注册代码为 "IRCT20151028024756N3",https://www.irct.ir/trial/29097?revision=114102。注册日期为 2019 年 12 月 16 日。
{"title":"Magnesium Supplementation Affects the Expression of Sirtuin1, Tumor Protein P53 and Endothelial Nitric Oxide Synthase Genes in Patients with Atherosclerosis: A Double-Blind, Randomized, Placebo-Controlled Trial.","authors":"Behnaz Rahnama Inchehsablagh, Farhad Ghadiri Soufi, Shabnaz Koochakkhani, Fariba Azarkish, Hossein Farshidi, Mahdiye Eslami, Masoumeh Mahmoodi, Nepton Soltani, Ebrahim Eftekhar","doi":"10.1007/s12291-022-01032-0","DOIUrl":"10.1007/s12291-022-01032-0","url":null,"abstract":"<p><p>Magnesium seems to play a role in improving cardiovascular function, but its exact mechanism is unknown. In this study, we hypothesized that magnesium could modulate the expression of genes involved in atherosclerosis. The aim of the present investigation was to evaluate the effect of magnesium sulfate on the expression of sirtuin1 (SIRT1), tumor protein p53 (TP53), and endothelial nitric oxide synthase (eNOS) genes in patients with atherosclerosis. This study was a placebo-controlled double-blind randomized clinical trial on 56 patients with angiographically proven atherosclerosis. Participants were randomly divided into two groups receiving 300 mg/day magnesium sulfate (n = 29) and placebo (n = 27) for three months (following up every month). Fasting blood samples were taken before and after the intervention and total RNA was extracted and used to evaluate the expression level of SIRT1, TP53, and eNOS genes by Real-Time PCR. The expression of eNOS gene was significantly increased (<i>P</i> < 0.0001) and the expression of TP53 gene was decreased (<i>P</i> = 0.02) in the magnesium sulfate group compared to the placebo group. But SIRT1 gene expression was not significantly different between the two groups. Our findings demonstrate that magnesium sulfate supplementation may have a protective role against the progression of atherosclerosis through upregulation of eNOS and downregulation of TP53 gene. <b>Trial registration</b>: This present clinical trial has been registered in the Iranian Registry of Clinical Trials (IRCT) with the registration code of \"IRCT20151028024756N3\", https://www.irct.ir/trial/29097?revision=114102. Registered on 16 December 2019.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"59-66"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10585905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dysregulation of Synaptic Plasticity Markers in Schizophrenia. 精神分裂症突触可塑性标记的失调
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-07-25 DOI: 10.1007/s12291-022-01068-2
Neha Keshri, Hanumanthappa Nandeesha

Schizophrenia is a mental disorder characterized by cognitive impairment resulting in compromised quality of life. Since the regulation of synaptic plasticity has functional implications in various aspects of cognition such as learning, memory, and neural circuit maturation, the dysregulation of synaptic plasticity is considered as a pathobiological feature of schizophrenia. The findings from our recently concluded studies indicate that there is an alteration in levels of synaptic plasticity markers such as neural cell adhesion molecule-1 (NCAM-1), Neurotropin-3 (NT-3) and Matrix-mettaloproteinase-9 (MMP-9) in schizophrenia patients. The objective of the present article is to review the role of markers of synaptic plasticity in schizophrenia. PubMed database (http;//www.ncbi.nlm.nih.gov/pubmed) was used to perform an extensive literature search using the keywords schizophrenia and synaptic plasticity. We conclude that markers of synaptic plasticity are altered in schizophrenia and may lead to complications of schizophrenia including cognitive dysfunction.

精神分裂症是一种以认知障碍为特征的精神疾病,会导致生活质量下降。由于突触可塑性的调节对学习、记忆和神经回路成熟等认知的各个方面都有功能性影响,因此突触可塑性的失调被认为是精神分裂症的一个病理生物学特征。我们最近的研究结果表明,精神分裂症患者的神经细胞粘附分子-1(NCAM-1)、神经营养素-3(NT-3)和基质金属蛋白酶-9(MMP-9)等突触可塑性标志物的水平发生了改变。本文旨在回顾突触可塑性标志物在精神分裂症中的作用。我们利用PubMed数据库(http;//www.ncbi.nlm.nih.gov/pubmed),以精神分裂症和突触可塑性为关键词进行了广泛的文献检索。我们的结论是,精神分裂症患者的突触可塑性标志物会发生改变,并可能导致包括认知功能障碍在内的精神分裂症并发症。
{"title":"Dysregulation of Synaptic Plasticity Markers in Schizophrenia.","authors":"Neha Keshri, Hanumanthappa Nandeesha","doi":"10.1007/s12291-022-01068-2","DOIUrl":"10.1007/s12291-022-01068-2","url":null,"abstract":"<p><p>Schizophrenia is a mental disorder characterized by cognitive impairment resulting in compromised quality of life. Since the regulation of synaptic plasticity has functional implications in various aspects of cognition such as learning, memory, and neural circuit maturation, the dysregulation of synaptic plasticity is considered as a pathobiological feature of schizophrenia. The findings from our recently concluded studies indicate that there is an alteration in levels of synaptic plasticity markers such as neural cell adhesion molecule-1 (NCAM-1), Neurotropin-3 (NT-3) and Matrix-mettaloproteinase-9 (MMP-9) in schizophrenia patients. The objective of the present article is to review the role of markers of synaptic plasticity in schizophrenia. PubMed database (http;//www.ncbi.nlm.nih.gov/pubmed) was used to perform an extensive literature search using the keywords schizophrenia and synaptic plasticity. We conclude that markers of synaptic plasticity are altered in schizophrenia and may lead to complications of schizophrenia including cognitive dysfunction.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"4-12"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10578521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Maternal Serum Zinc, Copper, Magnesium, and Iron in Spontaneous Abortions. 自然流产中母体血清中的锌、铜、镁和铁。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-05-03 DOI: 10.1007/s12291-022-01043-x
Sairoz, Krishnananda Prabhu, Vidyashree G Poojari, Sahana Shetty, Mahadeva Rao, Asha Kamath

Twenty five percent of pregnant women have some degree of vaginal bleeding during the first trimester, and about 50% of those pregnancies end in spontaneous abortion (SA) because the fetus is not developing typically. As studies have reported that inadequacies of trace metals such as Copper (Cu), Zinc (Zn), Magnesium (Mg) can predispose to various adverse pregnancy outcomes (PO); multiple micronutrient (MMN) supplementations are given without justifying their deficiency and toxicities on the fetus. Earlier studies on effects of MMN supplementations during pregnancy have not considered the need, duration, dose, and time of initiation of supplementations leading to inconclusive results. So, there is a need to optimize this to prevent their abuse and side effects. This study can help in establishing critical cut-offs of these minerals in maternal serum that can forecast future pregnancy outcomes. Study measured the serum Zn, Cu, Mg, and Fe in pregnant women who presented with (n = 80) and without (n = 100) SA at 5-2 weeks of pregnancy using iron -ferrozine method, magnesium-calmagite method, zinc reaction with nitro-PAPS, copper reaction with Di-Br- PAESA methods, respectively. Data analyzed using the student t test and cutoff value was established using Receiver Operating Characteristic (ROC) by SPSS software. Maternal serum Cu, Mg, Fe, and Zn levels measured were significantly lower in SA as compared to that of controls (p < 0.005) (Fig. 1) and maternal age and Body mass index were not statistically significant different among study group. Maternal serum Cu, Mg, Zn and Iron (Fe) measured in 5-12 weeks of pregnancy has the potential to forecast future occurrence of SA. The study has been registered under "The Clinical Trials Registry- India (CTRI)," -REF/2020/01/030393.

25% 的孕妇在怀孕头三个月会有不同程度的阴道出血,其中约 50% 的妊娠因胎儿发育不正常而以自然流产(SA)告终。有研究报告指出,微量金属元素如铜(Cu)、锌(Zn)、镁(Mg)的缺乏会导致各种不良妊娠结局(PO);因此,人们在没有证明微量金属元素缺乏及其对胎儿的毒性的情况下,就开始补充多种微量营养素(MMN)。早期关于孕期补充微量营养素影响的研究没有考虑补充的必要性、持续时间、剂量和开始补充的时间,导致结果不确定。因此,有必要对此进行优化,以防止其滥用和副作用。这项研究有助于确定母体血清中这些矿物质的临界值,从而预测未来的妊娠结果。研究采用铁-铁嗪法、镁-钙镁矿法、硝基-PAPS锌反应法、Di-Br- PAESA铜反应法,分别测量了怀孕5-2周时出现SA的孕妇(n = 80)和未出现SA的孕妇(n = 100)的血清锌、铜、镁和铁含量。数据采用学生 t 检验进行分析,临界值采用 SPSS 软件的接收者操作特征(ROC)确定。与对照组相比,SA 孕妇血清中铜、镁、铁和锌的含量明显较低(p
{"title":"Maternal Serum Zinc, Copper, Magnesium, and Iron in Spontaneous Abortions.","authors":"Sairoz, Krishnananda Prabhu, Vidyashree G Poojari, Sahana Shetty, Mahadeva Rao, Asha Kamath","doi":"10.1007/s12291-022-01043-x","DOIUrl":"10.1007/s12291-022-01043-x","url":null,"abstract":"<p><p>Twenty five percent of pregnant women have some degree of vaginal bleeding during the first trimester, and about 50% of those pregnancies end in spontaneous abortion (SA) because the fetus is not developing typically. As studies have reported that inadequacies of trace metals such as Copper (Cu), Zinc (Zn), Magnesium (Mg) can predispose to various adverse pregnancy outcomes (PO); multiple micronutrient (MMN) supplementations are given without justifying their deficiency and toxicities on the fetus. Earlier studies on effects of MMN supplementations during pregnancy have not considered the need, duration, dose, and time of initiation of supplementations leading to inconclusive results. So, there is a need to optimize this to prevent their abuse and side effects. This study can help in establishing critical cut-offs of these minerals in maternal serum that can forecast future pregnancy outcomes. Study measured the serum Zn, Cu, Mg, and Fe in pregnant women who presented with (<i>n</i> = 80) and without (<i>n</i> = 100) SA at 5-2 weeks of pregnancy using iron -ferrozine method, magnesium-calmagite method, zinc reaction with nitro-PAPS, copper reaction with Di-Br- PAESA methods, respectively. Data analyzed using the student t test and cutoff value was established using Receiver Operating Characteristic (ROC) by SPSS software. Maternal serum Cu, Mg, Fe, and Zn levels measured were significantly lower in SA as compared to that of controls (<i>p</i> < 0.005) (Fig. 1) and maternal age and Body mass index were not statistically significant different among study group. Maternal serum Cu, Mg, Zn and Iron (Fe) measured in 5-12 weeks of pregnancy has the potential to forecast future occurrence of SA. The study has been registered under \"The Clinical Trials Registry- India (CTRI),\" -REF/2020/01/030393.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"128-131"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10578523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Maternal and Newborn Blood Aluminum Levels and Neurodevelopment of Infants: Is there a Need for Concern? 孕产妇和新生儿血液中的铝含量与婴儿的神经发育:是否需要关注?
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2021-09-29 DOI: 10.1007/s12291-021-01002-y
Dolat Singh Shekhawat, Pratibha Singh, Vikash Chandra Janu, Praveen Sharma, Kuldeep Singh

Aluminum is a neurotoxic element that enters the human body due to its widespread usage in daily life. It has the potential to affect the neurological development of the fetus and infant adversely. This study aimed to evaluate the relationship between maternal and umbilical cord serum aluminum level and infant neurodevelopment. Over a period of March 2018 to September 2019, we conducted a prospective cohort study; 173 Mother-new-born pairs were enrolled. Aluminum levels were measured using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The correlation with Bayley Scales of Infant Development (BSID) -3rd edition score and maternal and cord serum aluminum were assessed via linear regression model. The mean concentration of maternal and cord serum aluminum was 2.58 ± 1.14 µg/dL and 1.44 ± 0.62 µg/dL, respectively. There was a significant correlation in aluminum level between maternal and umbilical cord serum (Pearson's r = 0.591, p < 0.000). There is no significant correlation between maternal and serum aluminum level, and BSID-3rd edition (cognitive, motor, language, and social-emotion) score at the average age of 6.5 months. In conclusion, maternal and cord serum aluminum levels were significantly correlated but did not correlate with infant neurodevelopment. Thus, low serum aluminum concentration and their association with child neurodevelopment deserve further investigation longitudinally in a large cohort.

Graphical abstract:

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-021-01002-y.

铝是一种神经毒性元素,由于在日常生活中的广泛使用而进入人体。它有可能对胎儿和婴儿的神经系统发育产生不利影响。本研究旨在评估母体和脐带血清铝水平与婴儿神经发育之间的关系。在 2018 年 3 月至 2019 年 9 月期间,我们开展了一项前瞻性队列研究;共招募了 173 对母婴。采用电感耦合等离子体光学发射光谱法(ICP-OES)测量铝含量。通过线性回归模型评估了贝利婴儿发展量表(BSID)第 3 版评分与母体和脐带血清铝的相关性。母体和脐带血清铝的平均浓度分别为 2.58 ± 1.14 µg/dL 和 1.44 ± 0.62 µg/dL。在平均 6.5 个月大时,母体和脐带血清中的铝含量存在明显相关性(Pearson's r = 0.591,p rd)。总之,母体和脐带血清铝水平与婴儿的神经发育有显著相关性,但没有相关性。因此,低血清铝浓度及其与儿童神经发育的关系值得在大型队列中进行纵向研究:在线版本包含补充材料,可在 10.1007/s12291-021-01002-y。
{"title":"Maternal and Newborn Blood Aluminum Levels and Neurodevelopment of Infants: Is there a Need for Concern?","authors":"Dolat Singh Shekhawat, Pratibha Singh, Vikash Chandra Janu, Praveen Sharma, Kuldeep Singh","doi":"10.1007/s12291-021-01002-y","DOIUrl":"10.1007/s12291-021-01002-y","url":null,"abstract":"<p><p>Aluminum is a neurotoxic element that enters the human body due to its widespread usage in daily life. It has the potential to affect the neurological development of the fetus and infant adversely. This study aimed to evaluate the relationship between maternal and umbilical cord serum aluminum level and infant neurodevelopment. Over a period of March 2018 to September 2019, we conducted a prospective cohort study; 173 Mother-new-born pairs were enrolled. Aluminum levels were measured using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The correlation with Bayley Scales of Infant Development (BSID) -3rd edition score and maternal and cord serum aluminum were assessed via linear regression model. The mean concentration of maternal and cord serum aluminum was 2.58 ± 1.14 µg/dL and 1.44 ± 0.62 µg/dL, respectively. There was a significant correlation in aluminum level between maternal and umbilical cord serum (Pearson's r = 0.591, p < 0.000). There is no significant correlation between maternal and serum aluminum level, and BSID-3<sup>rd</sup> edition (cognitive, motor, language, and social-emotion) score at the average age of 6.5 months. In conclusion, maternal and cord serum aluminum levels were significantly correlated but did not correlate with infant neurodevelopment. Thus, low serum aluminum concentration and their association with child neurodevelopment deserve further investigation longitudinally in a large cohort.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12291-021-01002-y.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"136-141"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10585903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of C4b as an adjunct marker in symptomatic RT-PCR negative Covid-19 cases. C4b作为症状性RT-PCR阴性Covid-19病例辅助标志物的评价
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-06-20 DOI: 10.1007/s12291-022-01033-z
Bandana Kumari, Krishnan Hajela, Asgar Ali, Abhay Kumar Sharma, Rajesh Kumar Yadav, Alok Ranjan, Rathish Nair, Shreekant Bharti, Satish Dipankar, Prabhat Kumar Singh, Sadhana Sharma

Introduction: Detecting low viral load has been a challenge in this pandemic, which has led to its escalated transmission. Complement activation has been implicated in pathogenesis of Covid-19 infection. Thus, evaluation of complement activation in suspected Covid-19 infection may help to detect infection and limit false negative cases thus limiting transmission of infection. We speculate that measuring C4b, produced from an activated complement system due to the presence of Covid-19 may help in its detection, even when the viral titers are low.

Methods: Plasma C4b levels of symptomatic RT-PCR positive patients (cases, n = 40); symptomatic RT-PCR negative patients (n = 35) and asymptomatic RT-PCR negative controls (n = 40) were evaluated. Plasma C5b-9, IL-6, D-dimer and C1-Inhibitor (C1-INH) were also measured in cases and controls. ELISA kits were used for all measurements. Statistical analyses were carried out using Stata, version 12 (Stata Corp., Texas, USA).

Results: C4b levels were found to be significantly increased in RT-PCR positive patients as compared to asymptomatic RT-PCR negative controls. RT-PCR negative but symptomatic patients still showed increased C4b levels. The significantly higher levels of C4b in cases with a cut-off value of ≥ 116 ng/ml with optimum sensitivity and specificity of 80% and 52% respectively is indicative of its possible use as an adjunct marker. Increased levels of D-dimer, IL6, along with decreased levels of C1-INH were found in cases compared to controls. Whereas, C5b-9 levels were not significantly raised in cases.

Conclusions: The results of our study suggests that plasma C4b may help to detect infection in false negative cases of RT-PCR that escape detection owing to low viral load. However, to confirm it a large-scale study is needed.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-022-01033-z.

引言:在这场导致其传播升级的疫情中,检测低病毒载量一直是一项挑战。补体激活与新冠肺炎感染的发病机制有关。因此,对疑似新冠肺炎感染中补体激活的评估可能有助于检测感染并限制假阴性病例,从而限制感染的传播。我们推测,即使在病毒滴度较低的情况下,测量因新冠肺炎的存在而激活的补体系统产生的C4b也可能有助于检测。方法:有症状的RT-PCR阳性患者(例 = 40);症状性RT-PCR阴性患者(n = 35)和无症状RT-PCR阴性对照(n = 40)进行评价。病例和对照组的血浆C5b-9、IL-6、D-二聚体和C1抑制剂(C1-INH)也进行了测量。ELISA试剂盒用于所有测量。使用Stata第12版(Stata Corp.,Texas,USA)进行统计分析。结果:与无症状RT-PCR阴性对照组相比,RT-PCR阳性患者的C4b水平显著升高。RT-PCR阴性但有症状的患者仍显示C4b水平升高。在临界值≥ 116ng/ml,最佳灵敏度和特异性分别为80%和52%,表明其可能用作辅助标记。与对照组相比,病例中发现D-二聚体IL6水平升高,C1-INH水平降低。然而,C5b-9水平在病例中没有显著升高。结论:我们的研究结果表明,血浆C4b可能有助于检测RT-PCR假阴性病例的感染,这些病例因病毒载量低而逃避检测。然而,要证实这一点,还需要进行大规模的研究。补充信息:在线版本包含补充材料,可访问10.1007/s12291-022-1033-z。
{"title":"Evaluation of C4b as an adjunct marker in symptomatic RT-PCR negative Covid-19 cases.","authors":"Bandana Kumari,&nbsp;Krishnan Hajela,&nbsp;Asgar Ali,&nbsp;Abhay Kumar Sharma,&nbsp;Rajesh Kumar Yadav,&nbsp;Alok Ranjan,&nbsp;Rathish Nair,&nbsp;Shreekant Bharti,&nbsp;Satish Dipankar,&nbsp;Prabhat Kumar Singh,&nbsp;Sadhana Sharma","doi":"10.1007/s12291-022-01033-z","DOIUrl":"10.1007/s12291-022-01033-z","url":null,"abstract":"<p><strong>Introduction: </strong>Detecting low viral load has been a challenge in this pandemic, which has led to its escalated transmission. Complement activation has been implicated in pathogenesis of Covid-19 infection. Thus, evaluation of complement activation in suspected Covid-19 infection may help to detect infection and limit false negative cases thus limiting transmission of infection. We speculate that measuring C4b, produced from an activated complement system due to the presence of Covid-19 may help in its detection, even when the viral titers are low.</p><p><strong>Methods: </strong>Plasma C4b levels of symptomatic RT-PCR positive patients (cases, n = 40); symptomatic RT-PCR negative patients (n = 35) and asymptomatic RT-PCR negative controls (n = 40) were evaluated. Plasma C5b-9, IL-6, D-dimer and C1-Inhibitor (C1-INH) were also measured in cases and controls. ELISA kits were used for all measurements. Statistical analyses were carried out using Stata, version 12 (Stata Corp., Texas, USA).</p><p><strong>Results: </strong>C4b levels were found to be significantly increased in RT-PCR positive patients as compared to asymptomatic RT-PCR negative controls. RT-PCR negative but symptomatic patients still showed increased C4b levels. The significantly higher levels of C4b in cases with a cut-off value of ≥ 116 ng/ml with optimum sensitivity and specificity of 80% and 52% respectively is indicative of its possible use as an adjunct marker. Increased levels of D-dimer, IL6, along with decreased levels of C1-INH were found in cases compared to controls. Whereas, C5b-9 levels were not significantly raised in cases.</p><p><strong>Conclusions: </strong>The results of our study suggests that plasma C4b may help to detect infection in false negative cases of RT-PCR that escape detection owing to low viral load. However, to confirm it a large-scale study is needed.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12291-022-01033-z.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"102-109"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9120313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of Klotho Protein in Neuropsychiatric Disorders: A Narrative Review. Klotho 蛋白在神经精神疾病中的作用:叙述性综述。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-08-13 DOI: 10.1007/s12291-022-01078-0
Amandeep Birdi, Sojit Tomo, Dharmveer Yadav, Praveen Sharma, Naresh Nebhinani, Prasenjit Mitra, Mithu Banerjee, Purvi Purohit

Neuropsychiatric disorders are comprised of diseases having both the neurological and psychiatric manifestations. The increasing burden of the disease on the population worldwide makes it necessary to adopt measures to decrease the prevalence. The Klotho is a single pass transmembrane protein that decreases with age, has been associated with various pathological diseases, like reduced bone mineral density, cardiac problems and cognitive impairment. However, multiple studies have explored its role in different neuropsychiatric disorders. A comprehensive search was undertaken in the Pubmed database for articles with the keywords "Klotho" and "neuropsychiatric disorders". The available literature, based on the above search strategy, has been compiled in this brief narrative review to describe the emerging role of Klotho in various neuropsychiatric disorders. The Klotho levels were decreased in various neuropsychiatric disorders except for bipolar disorder. A suppressed Klotho protein levels induced oxidative stress and incited pro-inflammatory conditions significantly contributing to the pathophysiology of neuropsychiatric disorder. The increasing evidence of altered Klotho protein levels in cognition-decrement-related disorders warrants its consideration as a biomarker in various neuropsychiatric diseases. However, further evidence is required to understand its role as a therapeutic target.

神经精神疾病包括神经和精神两方面的疾病。这种疾病给全球人口造成的负担日益加重,因此有必要采取措施降低发病率。Klotho 是一种单通道跨膜蛋白,会随着年龄的增长而减少,与多种病理疾病相关,如骨矿物质密度降低、心脏问题和认知障碍。不过,已有多项研究探讨了它在不同神经精神疾病中的作用。我们在 Pubmed 数据库中全面搜索了以 "Klotho "和 "神经精神疾病 "为关键词的文章。根据上述搜索策略,我们将现有文献整理成这篇简短的叙述性综述,以描述 Klotho 在各种神经精神疾病中的新作用。除躁狂症外,各种神经精神疾病中的 Klotho 水平都有所下降。被抑制的 Klotho 蛋白水平会诱发氧化应激和促炎症反应,这在很大程度上导致了神经精神疾病的病理生理学。越来越多的证据表明,Klotho 蛋白水平的改变与认知功能障碍有关,因此有必要将其视为各种神经精神疾病的生物标志物。然而,要了解其作为治疗靶点的作用,还需要进一步的证据。
{"title":"Role of Klotho Protein in Neuropsychiatric Disorders: A Narrative Review.","authors":"Amandeep Birdi, Sojit Tomo, Dharmveer Yadav, Praveen Sharma, Naresh Nebhinani, Prasenjit Mitra, Mithu Banerjee, Purvi Purohit","doi":"10.1007/s12291-022-01078-0","DOIUrl":"10.1007/s12291-022-01078-0","url":null,"abstract":"<p><p>Neuropsychiatric disorders are comprised of diseases having both the neurological and psychiatric manifestations. The increasing burden of the disease on the population worldwide makes it necessary to adopt measures to decrease the prevalence. The Klotho is a single pass transmembrane protein that decreases with age, has been associated with various pathological diseases, like reduced bone mineral density, cardiac problems and cognitive impairment. However, multiple studies have explored its role in different neuropsychiatric disorders. A comprehensive search was undertaken in the Pubmed database for articles with the keywords \"Klotho\" and \"neuropsychiatric disorders\". The available literature, based on the above search strategy, has been compiled in this brief narrative review to describe the emerging role of Klotho in various neuropsychiatric disorders. The Klotho levels were decreased in various neuropsychiatric disorders except for bipolar disorder. A suppressed Klotho protein levels induced oxidative stress and incited pro-inflammatory conditions significantly contributing to the pathophysiology of neuropsychiatric disorder. The increasing evidence of altered Klotho protein levels in cognition-decrement-related disorders warrants its consideration as a biomarker in various neuropsychiatric diseases. However, further evidence is required to understand its role as a therapeutic target.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"13-21"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10580745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Extracellular Vesicles (EVs) as "A Window to the Brain": Potential, Challenges and Future Perspectives. 作为 "大脑之窗 "的细胞外囊泡 (EV):潜力、挑战和未来展望。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2023-01-09 DOI: 10.1007/s12291-023-01111-w
Prasenjit Mitra, Shruti Gupta, Praveen Sharma
{"title":"Extracellular Vesicles (EVs) as \"A Window to the Brain\": Potential, Challenges and Future Perspectives.","authors":"Prasenjit Mitra, Shruti Gupta, Praveen Sharma","doi":"10.1007/s12291-023-01111-w","DOIUrl":"10.1007/s12291-023-01111-w","url":null,"abstract":"","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"1-3"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10585900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Impact of Covishield Vaccination in Terms of SARS CoV-2 Neutralizing Antibody Expression. 接种covshield对SARS CoV-2中和抗体表达的影响
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 DOI: 10.1007/s12291-022-01030-2
Rhema Elizabeth Thomas, Ajaikumar Sukumaran, Arun Krishnan R, Thushara Thomas, Biby T Edwin, P R Haritha, Bilha M Varghese, Jofy K Paul, Satheesh Kumar C S, D M Vasudevan

The vaccination efficacy can indirectly be assessed through the quantification of neutralizing antibodies. Very few data are available on Covishield efficacy in terms of neutralizing antibody expression upon vaccination. This study is focused on profiling of neutralizing antibody expression during and after the Covishield two shot vaccination and observing COVID-19 infection in vaccinated participants during the period. SARS CoV-2 neutralizing antibody concentrations in samples were estimated using electrochemiluminescence immunoassay kit for Lifotronics eCL8000. The sampling had been done sequentially at 45th, 85th day after 1st dose and 15th day after 2nd dose Covishield vaccination. Parallelly, in order to confirm the total SARS CoV-2 IgG response in COVID-19 infection, measured the IgG using SARS CoV-2 IgG lateral flow immunoassay test kit. The subjects previously infected with COVID-19 before 1st dose vaccination demonstrated high neutralizing antibody (> 10AU/ml). In COVID-19 uninfected subjects, there was a sudden incline in neutralizing antibody after the 2nd dose. Infection with SARS CoV-2 between 1st and 2nd dose of Covishield vaccination implicate that the level of neutralizing antibody in serum after 1st dose was not adequate to combat the virus and prevent infection. We observed COVID-19 infection in participants even after 2nd dose of vaccination. Interestingly, there was no protection against SARS CoV-2 even with a high neutralizing antibody expression of 188.5 AU/mL after the 2nd dose. Findings of Covishield efficacy in different cohort samples before and after 2 doses of Covishield vaccination provide impetus for improvement or development of next generation vaccines.

通过中和抗体的定量,可以间接评价疫苗接种效果。在疫苗接种后,很少有数据表明Covishield在中和抗体表达方面的有效性。本研究的重点是分析Covishield两次疫苗接种期间和之后的中和抗体表达,并观察接种参与者在此期间的COVID-19感染情况。使用Lifotronics eCL8000的电化学发光免疫测定试剂盒估计样品中SARS CoV-2中和抗体的浓度。接种第1剂后第45天、第85天、第2剂后第15天依次取样。同时,为了确认COVID-19感染时SARS CoV-2 IgG总应答,采用SARS CoV-2 IgG侧流免疫测定试剂盒检测IgG。首次接种前已感染COVID-19的受试者表现出较高的中和抗体(> 10AU/ml)。在未感染COVID-19的受试者中,第二次注射后,中和抗体出现突然倾斜。在第一次和第二次接种Covishield期间感染SARS CoV-2意味着第一次接种后血清中和抗体水平不足以对抗病毒和预防感染。我们观察到参与者在接种第二剂疫苗后仍有COVID-19感染。有趣的是,即使在第二次剂量后,中和抗体的表达达到188.5 AU/mL,也没有对SARS CoV-2产生保护作用。两剂Covishield疫苗接种前后不同队列样本的有效性研究结果为下一代疫苗的改进或开发提供了动力。
{"title":"Impact of Covishield Vaccination in Terms of SARS CoV-2 Neutralizing Antibody Expression.","authors":"Rhema Elizabeth Thomas,&nbsp;Ajaikumar Sukumaran,&nbsp;Arun Krishnan R,&nbsp;Thushara Thomas,&nbsp;Biby T Edwin,&nbsp;P R Haritha,&nbsp;Bilha M Varghese,&nbsp;Jofy K Paul,&nbsp;Satheesh Kumar C S,&nbsp;D M Vasudevan","doi":"10.1007/s12291-022-01030-2","DOIUrl":"https://doi.org/10.1007/s12291-022-01030-2","url":null,"abstract":"<p><p>The vaccination efficacy can indirectly be assessed through the quantification of neutralizing antibodies. Very few data are available on Covishield efficacy in terms of neutralizing antibody expression upon vaccination. This study is focused on profiling of neutralizing antibody expression during and after the Covishield two shot vaccination and observing COVID-19 infection in vaccinated participants during the period. SARS CoV-2 neutralizing antibody concentrations in samples were estimated using electrochemiluminescence immunoassay kit for Lifotronics eCL8000. The sampling had been done sequentially at 45th, 85th day after 1st dose and 15th day after 2nd dose Covishield vaccination. Parallelly, in order to confirm the total SARS CoV-2 IgG response in COVID-19 infection, measured the IgG using SARS CoV-2 IgG lateral flow immunoassay test kit. The subjects previously infected with COVID-19 before 1st dose vaccination demonstrated high neutralizing antibody (> 10AU/ml). In COVID-19 uninfected subjects, there was a sudden incline in neutralizing antibody after the 2nd dose. Infection with SARS CoV-2 between 1st and 2nd dose of Covishield vaccination implicate that the level of neutralizing antibody in serum after 1st dose was not adequate to combat the virus and prevent infection. We observed COVID-19 infection in participants even after 2nd dose of vaccination. Interestingly, there was no protection against SARS CoV-2 even with a high neutralizing antibody expression of 188.5 AU/mL after the 2nd dose. Findings of Covishield efficacy in different cohort samples before and after 2 doses of Covishield vaccination provide impetus for improvement or development of next generation vaccines.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"51-58"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9106397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Serum Holotranscobalamin with Serum Vitamin B12 in Population Prone to Megaloblastic Anemia and their Correlation with Nerve Conduction Study. 易患巨幼红细胞性贫血人群血清全曲钴胺与血清维生素 B12 的比较及其与神经传导研究的相关性。
IF 2.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-01 Epub Date: 2022-03-17 DOI: 10.1007/s12291-022-01027-x
Abhishek Verma, Sunita Aggarwal, Sandeep Garg, Smita Kaushik, Debashish Chowdhury

Early detection of megaloblastic anemia and associated neurological complications is crucial for management. This study was conducted to compare serum holotranscobalamin level with serum vitamin B12 level as early biomarker in people prone to megaloblastic anemia and to evaluate co-relation between these biomarkers and nerve conduction study in study patients. 83 adult patients (Hb > 12 gm/dl) prone to megaloblastic anemia were studied for basic haematological investigations, random blood sugar, thyroid function test, liver function test, kidney function test, serum vitamin B12, serum holotranscobalamin and serum folic acid levels. 45 patients among them underwent nerve conduction studies. All study patients were classified in 6 groups on the basis of risk factors for megaloblastic anemia. 29 patients (34.9%) were on antiepileptic drugs, 26 (31.3%) were chronic alcoholic, 10 patients (12%) each, had malabsorption and ileal tuberculosis, 6 (7.22%) had chronic pancreatitis and 2 (2.4%) had ileal resection. 30 patients (36.14%) had low serum holotranscobalamin, including 7 patients (8.43%) with low serum vitamin B12 level also, unmasking vitamin B12 deficiency in 23 patients (27.7%). 7 patients (8.43%) had mean corpuscular volume (MCV) > 100fL and 8 patients (9.63%) had vitamin B12 deficiency related changes on peripheral smear. Serum vitamin B12 and holotranscobalamin levels were significantly low in patients with peripheral smear changes, with p value 0.039 and 0.041 respectively, while no such association seen with MCV. Subclinical peripheral neuropathy was detected in 18 (40%) out of 45 patients on nerve conduction study. Serum holotranscobalamin levels were significantly lower (p = 0.031) than serum vitamin B12 levels (p = 0.2) in patients with neuropathic changes. Rest investigations and serum folic acid levels were normal in all patients. Holotranscobalamin levels can be considered early and reliable marker for vitamin B12 deficiency and deficiency associated peripheral neuropathy, even in patients who are prone to megaloblastic anemia, and not yet anemic or symptomatic for neuropathy.

早期发现巨幼细胞性贫血及相关神经系统并发症对于治疗至关重要。本研究旨在比较血清全转铁蛋白水平和血清维生素 B12 水平作为巨幼细胞性贫血患者的早期生物标志物,并评估这些生物标志物与患者神经传导研究之间的相关性。研究人员对 83 名易患巨幼细胞性贫血的成年患者(血红蛋白大于 12 克/分升)进行了基本血液学检查、随机血糖、甲状腺功能检测、肝功能检测、肾功能检测、血清维生素 B12、血清全转铁蛋白和血清叶酸水平检测。其中 45 名患者接受了神经传导检查。根据巨幼细胞贫血的风险因素,所有研究对象被分为 6 组。29名患者(34.9%)服用过抗癫痫药物,26名患者(31.3%)长期酗酒,10名患者(12%)患有吸收不良和回肠结核,6名患者(7.22%)患有慢性胰腺炎,2名患者(2.4%)进行过回肠切除术。30 名患者(36.14%)的血清全转录钴胺素水平较低,其中 7 名患者(8.43%)的血清维生素 B12 水平也较低,23 名患者(27.7%)的维生素 B12 缺乏。7 名患者(8.43%)的平均血球容积(MCV)大于 100fL,8 名患者(9.63%)的外周涂片出现与维生素 B12 缺乏相关的变化。有外周涂片变化的患者血清维生素 B12 和全血钴胺素水平明显偏低,P 值分别为 0.039 和 0.041,而 MCV 与维生素 B12 缺乏症无关联。45 名患者中有 18 人(40%)在神经传导检查中发现了亚临床周围神经病变。在有神经病变的患者中,血清全曲钴胺素水平(p = 0.031)明显低于血清维生素 B12 水平(p = 0.2)。所有患者的其他检查和血清叶酸水平均正常。即使是易患巨幼红细胞性贫血、尚未贫血或没有神经病变症状的患者,全血钴胺水平也可被视为维生素 B12 缺乏症和缺乏症相关周围神经病变的早期可靠标志物。
{"title":"Comparison of Serum Holotranscobalamin with Serum Vitamin B12 in Population Prone to Megaloblastic Anemia and their Correlation with Nerve Conduction Study.","authors":"Abhishek Verma, Sunita Aggarwal, Sandeep Garg, Smita Kaushik, Debashish Chowdhury","doi":"10.1007/s12291-022-01027-x","DOIUrl":"10.1007/s12291-022-01027-x","url":null,"abstract":"<p><p>Early detection of megaloblastic anemia and associated neurological complications is crucial for management. This study was conducted to compare serum holotranscobalamin level with serum vitamin B12 level as early biomarker in people prone to megaloblastic anemia and to evaluate co-relation between these biomarkers and nerve conduction study in study patients. 83 adult patients (Hb > 12 gm/dl) prone to megaloblastic anemia were studied for basic haematological investigations, random blood sugar, thyroid function test, liver function test, kidney function test, serum vitamin B12, serum holotranscobalamin and serum folic acid levels. 45 patients among them underwent nerve conduction studies. All study patients were classified in 6 groups on the basis of risk factors for megaloblastic anemia. 29 patients (34.9%) were on antiepileptic drugs, 26 (31.3%) were chronic alcoholic, 10 patients (12%) each, had malabsorption and ileal tuberculosis, 6 (7.22%) had chronic pancreatitis and 2 (2.4%) had ileal resection. 30 patients (36.14%) had low serum holotranscobalamin, including 7 patients (8.43%) with low serum vitamin B12 level also, unmasking vitamin B12 deficiency in 23 patients (27.7%). 7 patients (8.43%) had mean corpuscular volume (MCV) > 100fL and 8 patients (9.63%) had vitamin B12 deficiency related changes on peripheral smear. Serum vitamin B12 and holotranscobalamin levels were significantly low in patients with peripheral smear changes, with p value 0.039 and 0.041 respectively, while no such association seen with MCV. Subclinical peripheral neuropathy was detected in 18 (40%) out of 45 patients on nerve conduction study. Serum holotranscobalamin levels were significantly lower (<i>p</i> = 0.031) than serum vitamin B12 levels (<i>p</i> = 0.2) in patients with neuropathic changes. Rest investigations and serum folic acid levels were normal in all patients. Holotranscobalamin levels can be considered early and reliable marker for vitamin B12 deficiency and deficiency associated peripheral neuropathy, even in patients who are prone to megaloblastic anemia, and not yet anemic or symptomatic for neuropathy.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"38 1","pages":"42-50"},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10580748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Indian Journal of Clinical Biochemistry
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1