Silver fish (Mene maculata) is a type of pelagic fish found in the Moluccas sea, this fish has a high oil content so that it is easy to extract the oil content for transesterification. The transesterification reaction of oil from silver fish oil needs a catalyst, and heterogenous catalyst is a better choice. This study was determined the fatty acid methyl ester composition of M. maculata by transesterification process using heterogeneous CaO catalyst from chicken egg shells through 3 steps i.e. oil isolation, CaO catalyst synthesis, and oil transesterification reactions. Oil isolation was carried out by soxhletation method using petroleum ether with the oil content was 13.51%. Synthesis of CaO catalyst was carried out by calcination at 1000 °C for 2 hours and then charactererized by XRD. Fish oil is transesterified using CaO catalyst in a ratio of 11% (w/v) of the mixture. Furthermore, fatty acid methyl esters obtained were analyzed using GC-MS. The results showed 6 methyl ester fatty acids as composition of transesterification products which were methyl myristic (11.85%), methyl palmitoleate (8.71%), methyl palmitic (25.13%), methyl oleic (9.49%), methyl stearic (10.04%), and methyl 5,8,11,14-eikosatetraenoate (1.96%).
{"title":"Transesterifikasi Minyak Ikan Perak (Mene maculata) Dengan Katalis CaO Dari Cangkang Telur Ayam","authors":"Kartika Tahya, Candra Yulius Tahya, Healthy Kainama","doi":"10.30598//IJCR.2019.7-CAN","DOIUrl":"https://doi.org/10.30598//IJCR.2019.7-CAN","url":null,"abstract":"Silver fish (Mene maculata) is a type of pelagic fish found in the Moluccas sea, this fish has a high oil content so that it is easy to extract the oil content for transesterification. The transesterification reaction of oil from silver fish oil needs a catalyst, and heterogenous catalyst is a better choice. This study was determined the fatty acid methyl ester composition of M. maculata by transesterification process using heterogeneous CaO catalyst from chicken egg shells through 3 steps i.e. oil isolation, CaO catalyst synthesis, and oil transesterification reactions. Oil isolation was carried out by soxhletation method using petroleum ether with the oil content was 13.51%. Synthesis of CaO catalyst was carried out by calcination at 1000 °C for 2 hours and then charactererized by XRD. Fish oil is transesterified using CaO catalyst in a ratio of 11% (w/v) of the mixture. Furthermore, fatty acid methyl esters obtained were analyzed using GC-MS. The results showed 6 methyl ester fatty acids as composition of transesterification products which were methyl myristic (11.85%), methyl palmitoleate (8.71%), methyl palmitic (25.13%), methyl oleic (9.49%), methyl stearic (10.04%), and methyl 5,8,11,14-eikosatetraenoate (1.96%).","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81846028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.30598//IJCR.2019.7-PTB
P. Taba, Nadya Yuli Parmitha, Syahruddin Kasim
Silver nanoparticles have been synthesized by the reduction method using salam leaf extract (Syzygium polyanthum) as a reducing agent. The silver nanoparticles produced were then tested for their activity as antioxidants. The formation of silver nanoparticles was carried out by adding the leaf extract into the solution of AgNO3 and homogenized using a magnetic stirrer. A UV-Vis spectrophotometer was used to confirm the formation of silver nanoparticles. Particle Size Analyzer (PSA), Scanning Electron Microscope (SEM), X-Ray Diffractometer (XRD) and Fourir Transform Infra Red (FTIR) spectrometers were used to characterize the nanoparticles produced before being tested for antioxidant activity. The results showed that the absorbance value increased with increasing reaction contact time. The maximum uptake was obtained at wavelengths of 432-446 nm using a UV-Vis spectrophotometer. The particle size was determined using a PSA with an average particle size distribution of 45.7 nm. The average diameter of silver nanoparticles was 10.06 – 13.97 nm and the silver nanoparticles had rod-shapes. Functional groups that play a role in the synthesis of nanoparticles were –OH, –C=O, and –C-O groups. Silver nanoparticles inhibited free radicals as antioxidants with the IC50 value of 582.7 ppm.
{"title":"Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Salam (Syzygium polyanthum) Sebagai Bioreduktor Dan Uji Aktivitasnya Sebagai Antioksidan","authors":"P. Taba, Nadya Yuli Parmitha, Syahruddin Kasim","doi":"10.30598//IJCR.2019.7-PTB","DOIUrl":"https://doi.org/10.30598//IJCR.2019.7-PTB","url":null,"abstract":"Silver nanoparticles have been synthesized by the reduction method using salam leaf extract (Syzygium polyanthum) as a reducing agent. The silver nanoparticles produced were then tested for their activity as antioxidants. The formation of silver nanoparticles was carried out by adding the leaf extract into the solution of AgNO3 and homogenized using a magnetic stirrer. A UV-Vis spectrophotometer was used to confirm the formation of silver nanoparticles. Particle Size Analyzer (PSA), Scanning Electron Microscope (SEM), X-Ray Diffractometer (XRD) and Fourir Transform Infra Red (FTIR) spectrometers were used to characterize the nanoparticles produced before being tested for antioxidant activity. The results showed that the absorbance value increased with increasing reaction contact time. The maximum uptake was obtained at wavelengths of 432-446 nm using a UV-Vis spectrophotometer. The particle size was determined using a PSA with an average particle size distribution of 45.7 nm. The average diameter of silver nanoparticles was 10.06 – 13.97 nm and the silver nanoparticles had rod-shapes. Functional groups that play a role in the synthesis of nanoparticles were –OH, –C=O, and –C-O groups. Silver nanoparticles inhibited free radicals as antioxidants with the IC50 value of 582.7 ppm.","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"133 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75692572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.30598//IJCR.2019.7-RUS
Waode Rustiah, Alfian Noor, Maming Maming, Muhammad Lukman, N. Nurfadillah
The determination of lead (Pb) and cadmium (Cd) levels in sediment samples in Spermonde waters has been determined. Sampling was carried out in river and sea estuary waters. The method used in the analysis of Pb and Cd is the one specified by SNI 06-6992.3-2004 and SNI 06-6992.4-2004. Based on the Decree of the Minister of Environment No. 51 of 2004, the general condition of Spermonde waters still supports the activities of marine organisms. The results showed that Spermonde waters received more Pb metal input than Cd metal. Pb metal content in river estuary and sea waters averaged 17.38 ppm. The highest Pb metal content is found in Lanjukang Island and Bone Tambung Island, each ranging from 20.88 ppm and 20.19 ppm. Meanwhile, the lowest Pb metal content on Barang Lompo island was 9.86 ppm. The detected metal content of Cd is only found in the Barrang Lompo and Kondong islands of Bali, each ranging from 1.04 ppm and 0.19 ppm, while the other locations Cd metal are undetectable. Based on the SQG value, the content of Pb and Cd in sediments in Spermonde waters is still in the standard range for sediment that has not been contaminated.
{"title":"Distribusi Kandungan Logam Berat Pb dan Cd pada Sedimen Sepanjang Muara Sungai dan Laut Perairan Spermonde, Sulawesi Selatan, Indonesia","authors":"Waode Rustiah, Alfian Noor, Maming Maming, Muhammad Lukman, N. Nurfadillah","doi":"10.30598//IJCR.2019.7-RUS","DOIUrl":"https://doi.org/10.30598//IJCR.2019.7-RUS","url":null,"abstract":"The determination of lead (Pb) and cadmium (Cd) levels in sediment samples in Spermonde waters has been determined. Sampling was carried out in river and sea estuary waters. The method used in the analysis of Pb and Cd is the one specified by SNI 06-6992.3-2004 and SNI 06-6992.4-2004. Based on the Decree of the Minister of Environment No. 51 of 2004, the general condition of Spermonde waters still supports the activities of marine organisms. The results showed that Spermonde waters received more Pb metal input than Cd metal. Pb metal content in river estuary and sea waters averaged 17.38 ppm. The highest Pb metal content is found in Lanjukang Island and Bone Tambung Island, each ranging from 20.88 ppm and 20.19 ppm. Meanwhile, the lowest Pb metal content on Barang Lompo island was 9.86 ppm. The detected metal content of Cd is only found in the Barrang Lompo and Kondong islands of Bali, each ranging from 1.04 ppm and 0.19 ppm, while the other locations Cd metal are undetectable. Based on the SQG value, the content of Pb and Cd in sediments in Spermonde waters is still in the standard range for sediment that has not been contaminated. ","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75646205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.30598//IJCR.2019.7-FAS
F. A. Souhoka, Nikmans Hattu, Marsye Huliselan
Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxidant compound and an antioxidant activity of methanol extract of kesumba keling seeds. The moisture content of kesumba keling seeds is 78.74%. The powder of kesumba keling seeds was extracted using maceration method with 80% of methanol. Phytochemical test results of methanol extract positively contained flavonoid compound. An antioxidant activity test of methanol extract of kesumba keling seeds was carried out by determining DPPH free radical deterrent activity. The absorbance measurement were made using a UV-Vis spectrophotometer at a wavelength of 517 nm. Kesumba keling seeds extract has antioxidant activity which is indicated by IC50 value of 69.425 ppm, so it is classified as a strong antioxidant.
{"title":"Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)","authors":"F. A. Souhoka, Nikmans Hattu, Marsye Huliselan","doi":"10.30598//IJCR.2019.7-FAS","DOIUrl":"https://doi.org/10.30598//IJCR.2019.7-FAS","url":null,"abstract":"Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxidant compound and an antioxidant activity of methanol extract of kesumba keling seeds. The moisture content of kesumba keling seeds is 78.74%. The powder of kesumba keling seeds was extracted using maceration method with 80% of methanol. Phytochemical test results of methanol extract positively contained flavonoid compound. An antioxidant activity test of methanol extract of kesumba keling seeds was carried out by determining DPPH free radical deterrent activity. The absorbance measurement were made using a UV-Vis spectrophotometer at a wavelength of 517 nm. Kesumba keling seeds extract has antioxidant activity which is indicated by IC50 value of 69.425 ppm, so it is classified as a strong antioxidant.","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75730094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-31DOI: 10.30598//IJCR.2019.6-LAH
L. Harimu, La Rudi, Aceng Haetami, Giswa Ayu Pratiwi Santoso, Asriyanti .
A study of variations in the concentration of NaOH and H2SO4 to purify silica from rice husk ash and its application as adsorbent of Pb2+ and Cu2+ Metal Ions. The NaOH concentrations used were 8%, 9%, 10%, 11%, 12% and 13% and H2SO4 concentrations were 5.4%, 8.16%, 10.88%, and 13.61%. The process of adsorption of Pb2+ and Cu2+ metal ions was carried out at pH (3, 4, 5, 6) and silica masses. The results showed the silica yield obtained in the form of white powder. The highest silica content at 12% NaOH concentration of 41.81% with a purity of 89.09% and H2SO4 concentration is 10.88% at 65.39% with a purity of 94.94% initial content 86.02%. The results of surface structure characterization using Scanning Electron Microscope (SEM) average size of particle diameter was 0.030 µm. Optimal adsorption process of Pb2+ and Cu2+ metal ions using silica from rice husk occurred at pH 6 and the adsorbent mass was 0.3 gram, the adsorption efficiency was 98% and 86.25% respectively with adsorption capacity of 0.96 mg/g for metal Pb2+ and 0.83 mg/g for Cu2+ metals.
{"title":"Studi Variasi Konsentrasi NaOH dan H2SO4 Untuk Memurnikan Silika Dari Abu Sekam Padi Sebagai Adsorben Ion Logam Pb2+ dan Cu2+","authors":"L. Harimu, La Rudi, Aceng Haetami, Giswa Ayu Pratiwi Santoso, Asriyanti .","doi":"10.30598//IJCR.2019.6-LAH","DOIUrl":"https://doi.org/10.30598//IJCR.2019.6-LAH","url":null,"abstract":"A study of variations in the concentration of NaOH and H2SO4 to purify silica from rice husk ash and its application as adsorbent of Pb2+ and Cu2+ Metal Ions. The NaOH concentrations used were 8%, 9%, 10%, 11%, 12% and 13% and H2SO4 concentrations were 5.4%, 8.16%, 10.88%, and 13.61%. The process of adsorption of Pb2+ and Cu2+ metal ions was carried out at pH (3, 4, 5, 6) and silica masses. The results showed the silica yield obtained in the form of white powder. The highest silica content at 12% NaOH concentration of 41.81% with a purity of 89.09% and H2SO4 concentration is 10.88% at 65.39% with a purity of 94.94% initial content 86.02%. The results of surface structure characterization using Scanning Electron Microscope (SEM) average size of particle diameter was 0.030 µm. Optimal adsorption process of Pb2+ and Cu2+ metal ions using silica from rice husk occurred at pH 6 and the adsorbent mass was 0.3 gram, the adsorption efficiency was 98% and 86.25% respectively with adsorption capacity of 0.96 mg/g for metal Pb2+ and 0.83 mg/g for Cu2+ metals.","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90071871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-31DOI: 10.30598//ijcr.2019.6-nel
M. R. Sohilait, Nelson Gaspersz
In silico studies on interactions between the human pancreatic α-amylase (HPA) enzyme with α, β, and γ-mangostin ligands has been carried out using the molecular docking method. Ligands α, β, and γ-mangostin interact through the formation of hydrogen and van der waals bonds with residues on the enzyme active side. The α-mangostin ligands form seven hydrogen and six van der waals bonds with residues involved were Trp59, Gln63, Trp96, Thr163, Thr164, Ala198, His201, Glu233, and Asp300; β-mangostin forms five hydrogen and eight van der waals bonds with residues involved were Gln63, Trp96, Thr163, Thr164, Arg195, Asp197, His201, Glu233, Asp300, and His305; while γ-mangostin forms nine hydrogen and five van der waals bonds with residues involved were Trp59, Gln63, Trp96, Thr163, Asp197, Ala198, His201, Glu233, and Asp300. The binding afinity of α, β, and γ-mangostin to the HPA obtained were -7.0; -6.6; and -7.4 kcal/mol with RMSD value were 1,850; 1,956; and 1,811 Å, respectively. The number of hydrogen bonds that can be formed was responsible to the binding affinity. Ligand γ-mangostin has potential activity as an inhibitor of HPA enzyme due to the stable complexes formation with lower binding affinity (validated with RMSD value) when compared to α and β-mangostin.
{"title":"Penambatan Molekuler α, β, dan γ-mangostin Sebagai Inhibitor α-amilase Pankreas Manusia","authors":"M. R. Sohilait, Nelson Gaspersz","doi":"10.30598//ijcr.2019.6-nel","DOIUrl":"https://doi.org/10.30598//ijcr.2019.6-nel","url":null,"abstract":"In silico studies on interactions between the human pancreatic α-amylase (HPA) enzyme with α, β, and γ-mangostin ligands has been carried out using the molecular docking method. Ligands α, β, and γ-mangostin interact through the formation of hydrogen and van der waals bonds with residues on the enzyme active side. The α-mangostin ligands form seven hydrogen and six van der waals bonds with residues involved were Trp59, Gln63, Trp96, Thr163, Thr164, Ala198, His201, Glu233, and Asp300; β-mangostin forms five hydrogen and eight van der waals bonds with residues involved were Gln63, Trp96, Thr163, Thr164, Arg195, Asp197, His201, Glu233, Asp300, and His305; while γ-mangostin forms nine hydrogen and five van der waals bonds with residues involved were Trp59, Gln63, Trp96, Thr163, Asp197, Ala198, His201, Glu233, and Asp300. The binding afinity of α, β, and γ-mangostin to the HPA obtained were -7.0; -6.6; and -7.4 kcal/mol with RMSD value were 1,850; 1,956; and 1,811 Å, respectively. The number of hydrogen bonds that can be formed was responsible to the binding affinity. Ligand γ-mangostin has potential activity as an inhibitor of HPA enzyme due to the stable complexes formation with lower binding affinity (validated with RMSD value) when compared to α and β-mangostin.","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"74 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91480548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-31DOI: 10.30598//IJCR.2019.6-AMA
Rona Maningting Napitupulu, D. Julia, A. S. Panggabean
Validation method on the determination of Mn in lubricating oil by direct dilution method using Atomic Absorption Spectrophotometer (AAS) in Laboratory & Environment Control PT. Badak NGL Bontang has been done The validation method was done with research stages such as determination of optimum solvent, and determination of some important parameters influential for validation method such as parameter of liniearitas (r), Instrument Detection Limit (IDL), Methode Detection Limit (MDL), accuracy, precision, Limit of Detection(LOD) and Limit of Quantitation(LOQ). The result of research obtain is good, showed that the linearity value with R2 ≥ 0,997. The IDL and MDL value was 0.0021 ppm and 0.0092 ppm respectively and has been acceptability requirements of MDL. The accuracy paramater obtained recovery value with range 82.25-88.34%. The measurement of repeatability and reproducibility, the CV Horwitz value smaller than % RSD, indicating the method had a good precision. The measurement of LOD and LOQ value was 0.095 ppm 0.317 ppm respectively. Based on the result of the research determination method of Mn in lubricating oil by direct dilution method using AAS concluded valid.
{"title":"Validasi Metode Penentuan Mn Dalam Oli Lubrikan Dengan Metode Pengenceran Langsung Menggunakan Spektrofotometer Serapan Atom","authors":"Rona Maningting Napitupulu, D. Julia, A. S. Panggabean","doi":"10.30598//IJCR.2019.6-AMA","DOIUrl":"https://doi.org/10.30598//IJCR.2019.6-AMA","url":null,"abstract":"Validation method on the determination of Mn in lubricating oil by direct dilution method using Atomic Absorption Spectrophotometer (AAS) in Laboratory & Environment Control PT. Badak NGL Bontang has been done The validation method was done with research stages such as determination of optimum solvent, and determination of some important parameters influential for validation method such as parameter of liniearitas (r), Instrument Detection Limit (IDL), Methode Detection Limit (MDL), accuracy, precision, Limit of Detection(LOD) and Limit of Quantitation(LOQ). The result of research obtain is good, showed that the linearity value with R2 ≥ 0,997. The IDL and MDL value was 0.0021 ppm and 0.0092 ppm respectively and has been acceptability requirements of MDL. The accuracy paramater obtained recovery value with range 82.25-88.34%. The measurement of repeatability and reproducibility, the CV Horwitz value smaller than % RSD, indicating the method had a good precision. The measurement of LOD and LOQ value was 0.095 ppm 0.317 ppm respectively. Based on the result of the research determination method of Mn in lubricating oil by direct dilution method using AAS concluded valid.","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89835503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-31DOI: 10.30598//IJCR.2019.6-THI
Yusthinus T. Male, Deddy W.S. Modok, C. A. Seumahu, Dominggus Malle
This research was conducted to find bacteria from the genus Acidithiobacillus ferrooxidans which isolated from mine acid water in the copper mining area on Wetar Island, Maluku Province. This bacterium will be utilized in the biohidrometallurgical process (bioleaching) for copper extraction in mining area. The results of this study indicate that bacteria can be isolated from mine acid water samples by enrichment method that was use liquid media from Leathen which is optimized using Trypton Soya Broth (TSB) and bacterial growth on solid media. The presence of Thiobacillussp bacteria was confirmed by the results of gene analysis using a 16S rRNA sequence showing the presence of mixed bacterial colonies but not a single colony.
{"title":"Isolasi Mikroba Dari Air Asam Tambang Pada Area Pertambangan Tembaga Di Pulau Wetar, Provinsi Maluku","authors":"Yusthinus T. Male, Deddy W.S. Modok, C. A. Seumahu, Dominggus Malle","doi":"10.30598//IJCR.2019.6-THI","DOIUrl":"https://doi.org/10.30598//IJCR.2019.6-THI","url":null,"abstract":"This research was conducted to find bacteria from the genus Acidithiobacillus ferrooxidans which isolated from mine acid water in the copper mining area on Wetar Island, Maluku Province. This bacterium will be utilized in the biohidrometallurgical process (bioleaching) for copper extraction in mining area. The results of this study indicate that bacteria can be isolated from mine acid water samples by enrichment method that was use liquid media from Leathen which is optimized using Trypton Soya Broth (TSB) and bacterial growth on solid media. The presence of Thiobacillussp bacteria was confirmed by the results of gene analysis using a 16S rRNA sequence showing the presence of mixed bacterial colonies but not a single colony.","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83696322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-31DOI: 10.30598//IJCR.2019.6-RUS
Rustam Musta, Laily Nurliana
Kinetic studies effectiveness clove leaf (Syzigium aromaticum) oil as antifungal Candida albicans have been done. The study have purpose to determine the reaction order, reaction constants (k) and relationship the concentration of clove leaf (Syzigium aromaticum) oil every time (At) with the initial concentration of of clove (Syzigium aromaticum) oil (Ao) and time (t) and equipped determination of the minimum concentration of clove leaf (Syzigium aromaticum)oil effective anti-fungus Candida albicans. The results shows the anti-fungal activity clove leaf (Syzigium aromaticum) oil on Candida albicans for each variation of the concentration 100%, 75%, 50% and 25% are 14.2 mm, 12.2 mm, 10.8 mm and 10.4 mm respectively. Reaction order as antifungal of the clove leaf (Syzigium aromaticum) oil on Candida albicans is 0.2112 with k = 5.0594. The minimum concentration of clove leaf (Syzigium aromaticum) oil as anti-fungal Candida albicans is 17.86%.
{"title":"Studi Kinetika Efektifitas Minyak Daun Cengkeh (Syzigium aromaticum) Sebagai Antifungi Candida albicans","authors":"Rustam Musta, Laily Nurliana","doi":"10.30598//IJCR.2019.6-RUS","DOIUrl":"https://doi.org/10.30598//IJCR.2019.6-RUS","url":null,"abstract":"Kinetic studies effectiveness clove leaf (Syzigium aromaticum) oil as antifungal Candida albicans have been done. The study have purpose to determine the reaction order, reaction constants (k) and relationship the concentration of clove leaf (Syzigium aromaticum) oil every time (At) with the initial concentration of of clove (Syzigium aromaticum) oil (Ao) and time (t) and equipped determination of the minimum concentration of clove leaf (Syzigium aromaticum)oil effective anti-fungus Candida albicans. The results shows the anti-fungal activity clove leaf (Syzigium aromaticum) oil on Candida albicans for each variation of the concentration 100%, 75%, 50% and 25% are 14.2 mm, 12.2 mm, 10.8 mm and 10.4 mm respectively. Reaction order as antifungal of the clove leaf (Syzigium aromaticum) oil on Candida albicans is 0.2112 with k = 5.0594. The minimum concentration of clove leaf (Syzigium aromaticum) oil as anti-fungal Candida albicans is 17.86%.","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82448359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-31DOI: 10.30598//IJCR.2019.6-HAS
Hasty Hamzah, Nurlia Yusuf
Moringa oleifera Lam. is ones of the multipurpose plants. All parts of the plants are useful to human life and efficacious as a medicine. Moringa oleifera Lam can grow in the lowlands and highlands to an altitude of 1000 masl. This study was conducted to determine the levels of iron (Fe) in Moringa oleifera Lam leaves that grew at different heights using Atomic Absorption Spectroscopy (AAS) the results shows the amount of iron (Fe) in the lowlands of 14 masl (Wameo Village) is 6.28 mg per 100 g of Moringa leaf, medium plains 58 masl (Kantalai Village) is 5.57 mg per 100 g of sample while in the highlands 318 masl (Kaisabu Subdistrict) is 3.86 mg per 100 g of sample. Factors that influence the amount of iron content in Moringa oleifera Lam leaves are height which further affects the intensity of sunlight, humidity. Other factors can be caused by soil conditions (texture, moisture, nutrient, and pH).
{"title":"Analisis Kandungan Zat besi (Fe) Pada Daun Kelor (Moringa oleifera Lam.) Yang Tumbuh dengan Ketinggian Berbeda di Daerah Kota Baubau","authors":"Hasty Hamzah, Nurlia Yusuf","doi":"10.30598//IJCR.2019.6-HAS","DOIUrl":"https://doi.org/10.30598//IJCR.2019.6-HAS","url":null,"abstract":"Moringa oleifera Lam. is ones of the multipurpose plants. All parts of the plants are useful to human life and efficacious as a medicine. Moringa oleifera Lam can grow in the lowlands and highlands to an altitude of 1000 masl. This study was conducted to determine the levels of iron (Fe) in Moringa oleifera Lam leaves that grew at different heights using Atomic Absorption Spectroscopy (AAS) the results shows the amount of iron (Fe) in the lowlands of 14 masl (Wameo Village) is 6.28 mg per 100 g of Moringa leaf, medium plains 58 masl (Kantalai Village) is 5.57 mg per 100 g of sample while in the highlands 318 masl (Kaisabu Subdistrict) is 3.86 mg per 100 g of sample. Factors that influence the amount of iron content in Moringa oleifera Lam leaves are height which further affects the intensity of sunlight, humidity. Other factors can be caused by soil conditions (texture, moisture, nutrient, and pH).","PeriodicalId":13392,"journal":{"name":"Indo. J. Chem. Res.","volume":"362 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82643705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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