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Does folate deficiency alter sperm parameters in infertile males? 叶酸缺乏会改变不育男性的精子参数吗?
Q3 Immunology and Microbiology Pub Date : 2023-05-17 DOI: 10.34172/ipp.2023.39480
Saeideh Sadat Alamdari, Maryam Derakhshan, Marzieh Derakhshan, Parvin Mahzouni
Introduction: There is not enough evidence regarding the influence of folate deficiency in the etiology of idiopathic male infertility. Objectives: The objective of the current study was to examine whether there is an association between serum levels of folate and sperm parameters among infertile males. Patients and Methods: This cross-sectional study was undertaken on a sample of infertile males referring to an infertility treatment clinic in Isfahan province, Iran. Semen parameters, comprising sperm concentration, progressive/total motility, morphology, semen leukocyte, agglutination, and vitality were assessed based on standard protocols. The sperm DNA fragmentation assay (SDFA) and sperm chromatin maturity assay (SCMA) were also assessed by the sperm chromatin dispersion and aniline-blue tests respectively. Results: In general, 70 infertile males including 46 with sufficient serum folate level and 24 with insufficient serum folate level were examined. Subjects with sufficient serum folate level had a significantly higher mean of sperm concentration, vitality, progressive motility, total motility, SDFA, and SCMA (P<0.05). Higher serum folate level was also associated with lower risk of abnormal sperm agglutination (OR: 0.09, 95% CI: 0.01-0.65), and morphology (OR: 0.12, 95% CI: (0.02-0.60) after adjustment for potential confounders. Conclusion: The present study found a significant association between serum folate level and infertility among Iranian infertile males. However, prospective large-scale population-based research is required to confirm the finding.
目前还没有足够的证据表明叶酸缺乏对特发性男性不育症病因的影响。目的:本研究的目的是研究在不育男性中血清叶酸水平和精子参数之间是否存在关联。患者和方法:这项横断面研究是在伊朗伊斯法罕省不孕不育治疗诊所的不育男性样本上进行的。根据标准方案评估精液参数,包括精子浓度、进行性/总运动性、形态、精液白细胞、凝集和活力。用精子染色质分散试验和苯胺蓝试验分别评价精子DNA片段化试验(SDFA)和精子染色质成熟度试验(SCMA)。结果:共检测70例不育男性,其中血清叶酸充足者46例,血清叶酸不足者24例。血清叶酸水平充足的受试者精子浓度、活力、进行性运动性、总运动性、SDFA和SCMA的平均值显著高于对照组(P<0.05)。在调整潜在混杂因素后,较高的血清叶酸水平也与较低的精子异常凝集风险(OR: 0.09, 95% CI: 0.01-0.65)和形态(OR: 0.12, 95% CI: 0.02-0.60)相关。结论:本研究发现伊朗不育男性血清叶酸水平与不育之间存在显著关联。然而,需要前瞻性的大规模人群研究来证实这一发现。
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引用次数: 0
The effect of delivery type (vaginal delivery versus cesarean section) on intraocular pressure and retinal nerve fiber layer thickness in healthy women 分娩方式(阴道分娩与剖宫产)对健康妇女眼压和视网膜神经纤维层厚度的影响
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-05-16 DOI: 10.34172/ipp.2023.39478
S. Nazari, A. Smaeili, A. Kasiri, Parivash Jelodarian
Introduction: Pregnancy imposes physiological stress and significantly changes human body. This change may have potential effects on visual system. Little is known about pregnancy effects on intraocular pressure (IOP) and glaucoma. Objectives: The present study tended to evaluate and compare the effect of normal vaginal delivery (NVD) versus cesarean section (CS) on IOP and retinal nerve fiber layer thickness (RNFLT) in healthy women. Patients and Methods: Sixty pregnant women participated in this prospective cross-sectional study. The participants were assigned to two groups; the first group consisted of 30 candidates for NVD and the second group of 30 candidates for CS. Then, IOP in three states before delivery, during delivery, and six weeks after delivery then RNFLT were measured and compared by optical coherence tomography (OCT) in two states before delivery and six weeks after delivery. Results: Based on the results, there was no significant difference in the RNFLT in left and right eyes, before and after delivery in the NVD and CS groups (P=0.081 and P=0.090, respectively). Additionally, following the comparison of NVD and SC groups, no significant difference in the RNFLT between two groups was detected (P>0.05). According to IOP measurements in both eyes, IOP during delivery was significantly higher than before and after delivery in NVD group (P=0.05 and P=0.048, respectively) and CS group (P=0.028 and P=0.035, respectively). In addition, IOP in NVD group was higher than CS group in all states, but no statistically significant difference was seen among them (P>0.05). Conclusion: In general, the results showed that delivery type does not significantly affect RNFLT and IOP. It is also possible that an increase in IOP will lead to different results in women with pre-existing glaucoma or risk factors for it. Therefore, in cases with a positive background of glaucoma, specialized examinations and delivery under the supervision of a specialist are recommended.
妊娠给人体带来生理压力,使人体发生显著变化。这种变化可能对视觉系统有潜在的影响。妊娠对眼内压(IOP)和青光眼的影响知之甚少。目的:本研究旨在评价和比较正常阴道分娩(NVD)与剖宫产(CS)对健康女性IOP和视网膜神经纤维层厚度(RNFLT)的影响。患者和方法:60名孕妇参与了这项前瞻性横断面研究。参与者被分为两组;第一组为30名NVD候选人,第二组为30名CS候选人。采用光学相干断层扫描(OCT)测量分娩前、分娩中、分娩后6周三个状态的IOP,并比较分娩前两个状态和分娩后6周的RNFLT。结果:结果显示,NVD组和CS组分娩前后左右眼RNFLT差异无统计学意义(P=0.081, P=0.090)。NVD组与SC组比较,两组RNFLT差异无统计学意义(P < 0.05)。双眼IOP测量结果显示,NVD组分娩时IOP明显高于分娩前后(P=0.05和P=0.048)和CS组(P=0.028和P=0.035)。NVD组IOP在各状态均高于CS组,但两组间差异无统计学意义(P < 0.05)。结论:一般情况下,分娩方式对RNFLT和IOP无显著影响。对于患有青光眼或有青光眼危险因素的女性,IOP的升高也可能导致不同的结果。因此,对于有青光眼阳性背景的病例,建议在专科医生的监督下进行专门检查和分娩。
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引用次数: 0
Silver resistance in Pseudomonas aeruginosa and Acinetobacter baumannii isolated from burn patients 烧伤患者铜绿假单胞菌和鲍曼不动杆菌对银的耐药性
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-05-16 DOI: 10.34172/ipp.2023.39498
F. Farajzadeh, K. Diba, J. Kazemzadeh, Shohreh Afshar Yavari
Introduction: Burn infections are a serious obstacle to the patient’s recovery. Infection is estimated to account for 75% of burn patient mortality. Widespread and often indiscriminate administration of antibiotics and lack of basic infection control methods are major factors in the emergence of drug-resistant bacteria. Due to the growing and serious threat of antibiotic resistance, interest in silver compounds has grown in modern medicine. Objectives: The aim of this descriptive study was to evaluate the frequency of sil genes and its phenotypic expression in Pseudomonas aeruginosa and Acinetobacter baumannii isolates isolated from the burn ward of Imam Khomeini hospital in Urmia. Patients and Methods: P. aeruginosa (n=16) and A. baumannii (n=32) isolates were collected from burn wound samples in an 8-month period from August to March 2017 from the burn ward of the hospital. The minimum inhibitory concentration of silver nitrate on the clinical isolates was determined using microdilution method. The presence of silP, silE and silS silver resistance genes was investigated by polymerase chain reaction (PCR). Results: The results of minimum inhibitory concentration (MIC) test showed that 62.5% (n = 10) of P. aeruginosa isolates and 56.25% (n = 18) of A. baumannii isolates showed MIC above 512 mg/ml. Polymerase chain reaction results revealed that only one P. aeruginosa isolate had silE gene and among A. baumannii isolates, 20 isolates had silE gene and six isolates had silS gene. None of the isolates showed positive results for the silP gene. Conclusion: Based on the results, A. baumannii was the most common microorganism of burn wounds in the burn ward of our hospital in Urmia. This study showed a high degree of phenotypic resistance to silver in A. baumannii and P. aeruginosa isolates which silE and silS genes were also observed in some isolates.
引言:烧伤感染是患者康复的严重障碍。据估计,感染占烧伤患者死亡率的75%。抗生素的广泛使用和滥用以及缺乏基本的感染控制方法是耐药细菌出现的主要因素。由于抗生素耐药性的日益严重的威胁,现代医学对银化合物的兴趣与日俱增。目的:本描述性研究的目的是评估从尿毒症伊玛目霍梅尼医院烧伤病房分离的铜绿假单胞菌和鲍曼不动杆菌中sil基因的频率及其表型表达。患者和方法:从2017年8月至3月的8个月时间内,从医院烧伤病房的烧伤伤口样本中采集铜绿假单胞菌(n=16)和鲍曼不动杆菌(n=32)分离株。采用微量稀释法测定硝酸银对临床分离株的最低抑菌浓度。用聚合酶链式反应(PCR)检测了silP、silE和silS银抗性基因的存在。结果:最小抑菌浓度(MIC)试验结果显示,62.5%(n=10)的铜绿假单胞菌和56.25%(n=18)的鲍曼不动杆菌MIC均在512mg/ml以上。聚合酶链反应结果显示,只有1株铜绿假单胞菌具有silE基因,鲍曼不动杆菌中有20株具有silE,6株具有silS基因。没有一个分离株显示出对silP基因的阳性结果。结论:鲍曼不动杆菌是我院尿毒症烧伤病区烧伤创面最常见的微生物。本研究表明,鲍曼不动杆菌和铜绿假单胞菌对银具有高度的表型抗性,在一些分离株中也观察到silE和silS基因。
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引用次数: 0
Neurological complications as the hallmark symptoms of thrombotic thrombocytopenic purpura in sickle cell disease 镰状细胞病血栓性血小板减少性紫癜的标志性症状是神经系统并发症
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-05-16 DOI: 10.34172/ipp.2023.39490
M. Bahadoram, B. Keikhaei, Shahram Rafie, E. Akade, Najmeh Nameh Goshay Fard, Roozbeh Moghaddar
Thrombotic thrombocytopenic purpura (TTP) is a type of microangiopathy, which is characterized by the formation of small blood clots in the vessels, leading to organ damage and low platelet count. In sickle cell disease (SCD), TTP can occur due to the activation of the coagulation system and damage to the endothelial cells lining the blood vessels. The importance of TTP in SCD lies in its potential to cause severe and life-threatening complications, including stroke, renal and heart failure. Early recognition and treatment of TTP in SCD are crucial to prevent these complications and improve outcomes. In this letter, we highlighted the significance of neurological complications in the diagnosis of TTP in SCD.
血栓性血小板减少性紫癜(TTP)是一种微血管疾病,其特点是血管中形成小血块,导致器官损伤和血小板计数低。在镰状细胞病(SCD)中,TTP可由于凝血系统的激活和血管内皮细胞的损伤而发生。TTP在SCD中的重要性在于它可能导致严重和危及生命的并发症,包括中风、肾衰竭和心力衰竭。SCD的TTP早期识别和治疗对于预防这些并发症和改善预后至关重要。在这封信中,我们强调了神经系统并发症在SCD TTP诊断中的重要性。
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引用次数: 0
Galbanic acid of Ferula assa-foetida L, as a regulator of the AMPK pathway in reduction of lipid accumulation in HepG2 cells 阿魏中Galbanic酸在减少HepG2细胞脂质积累中的AMPK通路调节作用
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-05-16 DOI: 10.34172/ipp.2023.39479
H. Musavi, H. Shokri-Afra, Prof. Dr. Soleiman Mahjoub, Abbas Khonakdar-Tarsi, A. Bagheri, Zahra Memariani
Introduction: Hepatic fat accumulation is a complication of non-alcoholic fatty liver disease (NAFLD). An AMP-activated protein kinase (AMPK) reduces the synthesis of fatty acids by inhibiting sterol regulatory element-binding transcription factor 1-c (SREBP1-C) and acetyl COA carboxylase (ACC). Objectives: We aimed to investigate the impress of galbanic acid (Gal) from the Ferula plant on AMPK and its inhibitory effect on lipogenic enzymes in HepG2 cells. Materials and Methods: In an applied-fundamental study, HepG2 cells were treated for 24 hours with Gal in palmitate (Pal). Resveratrol (RSV) was conducted as a positive control. Fatty acid synthase (FAS) and SREBP1-C gene expression were evaluated by reverse transcription polymerase chain reaction (RT-PCR). FAS, phospho-acetyl-CoA carboxylase (P-ACC), P-AMPK, AMPK, SREBP-1c, and ACC protein levels were measured by western blotting. Lipid accumulation was investigated qualitatively and semi-quantitatively with oil red. Results: The semi-quantitative results of oil revealed a substantial reduction (P<0.004) in lipid accumulation for treatment with Gal. The significant increase in the protein level of P-AMPK (P<0.001) and P-ACC (P=0.054) and significant decrease in FAS (P<0.003), SREBP-1c (P<0.001) and ACC (P<0.011) due to the effect galbanic acid was observed. FAS gene expression decreased significantly (P<0.009), while the decrease in SREBP-1c gene expression was not significant (P=0.303). Conclusion: These findings direct that galbanic acid can be a new regulator of AMPK. Hence, the present study may introduce galbanic acid as a new plan to positively regulate the AMPK pathway, which leads to the regulation of various cellular processes.
肝脏脂肪堆积是非酒精性脂肪性肝病(NAFLD)的并发症。amp激活的蛋白激酶(AMPK)通过抑制甾醇调节元件结合转录因子1-c (SREBP1-C)和乙酰辅酶a羧化酶(ACC)来减少脂肪酸的合成。目的:研究阿魏植物半胱甘酸(Gal)对HepG2细胞AMPK的影响及其对脂肪生成酶的抑制作用。材料与方法:在应用基础研究中,用棕榈酸Gal (Pal)处理HepG2细胞24小时。白藜芦醇(Resveratrol, RSV)作为阳性对照。反转录聚合酶链反应(RT-PCR)检测脂肪酸合成酶(FAS)和SREBP1-C基因表达。western blotting检测FAS、磷酸乙酰辅酶a羧化酶(P-ACC)、P-AMPK、AMPK、SREBP-1c和ACC蛋白水平。用油红对脂质积累进行了定性和半定量研究。结果:半定量结果显示,半胱甘酸能显著降低脂质积累(P<0.004)。半胱甘酸能显著提高P- ampk (P<0.001)和P-ACC (P=0.054)蛋白水平,显著降低FAS (P<0.003)、SREBP-1c (P<0.001)和ACC (P<0.011)蛋白水平。FAS基因表达明显降低(P<0.009), SREBP-1c基因表达降低不显著(P=0.303)。结论:这些发现提示半胱甘酸可能是一种新的AMPK调节因子。因此,本研究可能引入半胱氨酸作为正向调控AMPK通路的新方案,从而调控多种细胞过程。
{"title":"Galbanic acid of Ferula assa-foetida L, as a regulator of the AMPK pathway in reduction of lipid accumulation in HepG2 cells","authors":"H. Musavi, H. Shokri-Afra, Prof. Dr. Soleiman Mahjoub, Abbas Khonakdar-Tarsi, A. Bagheri, Zahra Memariani","doi":"10.34172/ipp.2023.39479","DOIUrl":"https://doi.org/10.34172/ipp.2023.39479","url":null,"abstract":"Introduction: Hepatic fat accumulation is a complication of non-alcoholic fatty liver disease (NAFLD). An AMP-activated protein kinase (AMPK) reduces the synthesis of fatty acids by inhibiting sterol regulatory element-binding transcription factor 1-c (SREBP1-C) and acetyl COA carboxylase (ACC). Objectives: We aimed to investigate the impress of galbanic acid (Gal) from the Ferula plant on AMPK and its inhibitory effect on lipogenic enzymes in HepG2 cells. Materials and Methods: In an applied-fundamental study, HepG2 cells were treated for 24 hours with Gal in palmitate (Pal). Resveratrol (RSV) was conducted as a positive control. Fatty acid synthase (FAS) and SREBP1-C gene expression were evaluated by reverse transcription polymerase chain reaction (RT-PCR). FAS, phospho-acetyl-CoA carboxylase (P-ACC), P-AMPK, AMPK, SREBP-1c, and ACC protein levels were measured by western blotting. Lipid accumulation was investigated qualitatively and semi-quantitatively with oil red. Results: The semi-quantitative results of oil revealed a substantial reduction (P<0.004) in lipid accumulation for treatment with Gal. The significant increase in the protein level of P-AMPK (P<0.001) and P-ACC (P=0.054) and significant decrease in FAS (P<0.003), SREBP-1c (P<0.001) and ACC (P<0.011) due to the effect galbanic acid was observed. FAS gene expression decreased significantly (P<0.009), while the decrease in SREBP-1c gene expression was not significant (P=0.303). Conclusion: These findings direct that galbanic acid can be a new regulator of AMPK. Hence, the present study may introduce galbanic acid as a new plan to positively regulate the AMPK pathway, which leads to the regulation of various cellular processes.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48710032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of HLA-B27 frequency in patients with ankylosing spondylitis, and its relationship with clinical symptoms in Khuzestan province 胡齐斯坦省强直性脊柱炎患者HLA-B27频率及其与临床症状的关系
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-05-16 DOI: 10.34172/ipp.2023.39472
Mahnoosh Davodi, F. Nokhostin, Elham Rajaei
Introduction: Ankylosing spondylitis (AS) is an autoimmune disease, which causes mild to severe clinical symptoms in patients. Several inherited and acquired factors are involved in the pathogenesis of the disease. Human leukocyte antigen B27 (HLA-B27) is one of the factors, whose expression affects patients’ susceptibility to AS. Objectives: In this study, we evaluated HLA-B27 frequency in AS patients of Khuzestan province of Iran. Patients and Methods: The study population (N=114) including patients with AS. Patients were examined and confirmed by a rheumatologist based on New York modified criteria. Clinical information was extracted from patients’ documents. Furthermore, general characteristics of the patients and several clinical variables (physio-pathological, self-report, and imaging) were taken into consideration. Results: The results showed that 62.3% of the patients were HLA-B27 positive, while 37.7% of which were HLA-B27 negative. This difference was statistically significant (P <0.05). The disease duration was shown to be almost equal in both groups (i.e., HLA-B27 positive and HLA-B27 negative). According to the results, various clinical symptoms (like ocular complications and peripheral arthritis) were observed in patients, and the complications of which were higher in HLA-B27 negative patients compared to HLA-B27 positive ones (P <0.05). Besides, it was seen that the male patients have shown a higher physio-pathological (using polymerase chain reaction [PCR] technique) report for positive HLA-B27 and a higher magnetic resonance imaging involvement compared with females, whereas the women showed higher disease activity score i.e., higher mean Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) value. Conclusion: For the AS patients, negative HLA-B27 cases show more progressive involvement sacroiliac joints compared with the positive HLA-B27 cases. More importantly, of hundred percent of patients who responded to anti-tumor necrosis factor (TNF) treatment, 52.6% were positive for the HLA-B27 antigen, and 47.4% were negative for the HLA-B27 antigen. Overall, it can be said that HLA-B27 lonely is not involved in the pathogenesis of AS and environmental factors should be investigated and involved in the disease development.
简介:强直性脊柱炎(AS)是一种自身免疫性疾病,可导致患者出现轻度至重度临床症状。几种遗传和后天因素参与了该病的发病机制。人类白细胞抗原B27(HLA-B27)是影响患者AS易感性的因素之一。目的:本研究评估了伊朗胡齐斯坦省AS患者的HLA-B27频率。患者和方法:研究人群(N=114)包括AS患者。患者由风湿病学家根据纽约改良标准进行检查和确认。临床信息是从患者的文件中提取的。此外,还考虑了患者的一般特征和几个临床变量(生理病理、自我报告和影像学)。结果:HLA-B27阳性者占62.3%,HLA-B27阴性者占37.7%。这一差异具有统计学意义(P<0.05)。两组的病程几乎相等(即HLA-B27阳性和HLA-B27阴性)。结果显示,患者有多种临床症状(如眼部并发症和周围性关节炎),其中HLA-B27阴性患者的并发症高于HLA-B27阳性患者(P<0.05),可以看出,与女性相比,男性患者显示出更高的HLA-B27阳性的生理病理学(使用聚合酶链式反应[PCR]技术)报告和更高的磁共振成像受累,而女性显示出更大的疾病活动性评分,即更高的巴斯强直性脊柱炎疾病活动性指数(BASDAI)平均值。结论:在AS患者中,HLA-B27阴性的患者比HLA-B27阳性的患者表现出更多的进行性侵犯骶髂关节。更重要的是,在100%对抗肿瘤坏死因子(TNF)治疗有反应的患者中,52.6%的患者HLA-B27抗原呈阳性,47.4%的患者HLA-B27抗原呈阴性。总之,可以说HLA-B27孤独与AS的发病机制无关,应调查环境因素并参与疾病的发展。
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引用次数: 0
Evaluation of the relation between cerebroplacental ratio, umbilical-cerebral ratio, and cerebro-placental-uterine ratio with the occurrence of adverse perinatal outcomes in pregnancies complicated by fetal growth restriction 评价脑胎盘比、脐脑比、脑胎盘子宫比与妊娠合并胎儿生长受限围产儿不良结局发生的关系
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-05-16 DOI: 10.34172/ipp.2023.39503
S. Khanjani, F. Farahbod, E. Zarean, M. Tarrahi, B. Mohammadi
Introduction: Fetal growth restriction (FGR) is a major obstetric complication associated with an increased risk of adverse perinatal outcomes. Objectives: This study aimed to evaluate the relationship between Doppler parameters, including the cerebroplacental ratio (CPR), umbilicocerebral ratio (UCR), and cerebro-placental-uterine ratio (CPUR), with adverse perinatal outcomes in singleton pregnancies complicated by FGR. Patients and Methods: This was a prospective study of 100 women with a singleton pregnancy 28 and 36.8 weeks of gestation was complicated by FGR and mild abnormalities. Feto-maternal Doppler examinations were conducted by the CPR, UCR, and CPUR parameters. Adverse outcomes were defined as Apgar score <7 at 5 minutes, preterm birth <37-week, neonatal intensive care unit (NICU) admission, fetal distress, and emergency cesarean section. These outcome parameters were checked with the results of the last ultrasound which performed 1-2 weeks before delivery. Results: Mean umbilical artery pulsatility index (UA-PI) (1.18±0.31 versus 1.04±0.21, P=0.010) and mean uterine arteries (UtAs)-PI (1.18±0.45 versus 0.96±0.36, P=0.20) were significantly higher in pregnancies that experienced adverse perinatal outcomes than those that did not experience them. Mean CPUR (1.82±1.03 versus 2.25±0.83, P=0.039) was significantly lower in pregnancies that experienced adverse perinatal outcomes versus those that did not. In binary multivariate logistic regression analysis, CPR, UCR, and CPUR parameters were evaluated with adverse perinatal outcomes. Only CPUR had a significant relationship with adverse perinatal outcomes. CPUR had a substantial relationship with Apgar score <7 at 5 minutes (OR: 0.13; 95% CI: 0.02-0.63; P=0.012). Conclusion: CPUR is a new Doppler ratio associated with adverse perinatal outcomes in FGR pregnancies with minimal abnormalities.
胎儿生长受限(FGR)是与不良围产期结局风险增加相关的主要产科并发症。目的:本研究旨在评价单胎妊娠合并FGR患者的多普勒参数,包括脑胎盘比(CPR)、脐脑比(UCR)和脑胎盘子宫比(CPUR)与不良围产期结局的关系。患者和方法:这是一项前瞻性研究,100例单胎妊娠28周和36.8周合并FGR和轻度异常的妇女。通过心肺复苏术(CPR)、超声复苏术(UCR)和心肺复苏术(CPR)参数进行胎母多普勒检查。不良结局定义为5分钟时Apgar评分<7、早产<37周、新生儿重症监护病房(NICU)入院、胎儿窘迫和紧急剖宫产。这些结果参数与分娩前1-2周进行的最后一次超声结果进行核对。结果:围产期不良结局孕妇的平均脐动脉脉搏指数(UA-PI)(1.18±0.31比1.04±0.21,P=0.010)和平均子宫动脉指数(UtAs)-PI(1.18±0.45比0.96±0.36,P=0.20)显著高于未发生围产期不良结局孕妇。平均CPUR(1.82±1.03 vs 2.25±0.83,P=0.039)在经历不良围产期结局的妊娠中显著低于未经历不良围产期结局的妊娠。在二元多变量logistic回归分析中,CPR、UCR和CPUR参数与不良围产期结局进行评估。只有CPUR与围产期不良结局有显著关系。CPUR与5分钟Apgar评分<7有显著关系(OR: 0.13;95% ci: 0.02-0.63;P = 0.012)。结论:CPUR是一种新的多普勒比值,与FGR妊娠的不良围产期结局有关。
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引用次数: 0
Comparison of pregnancy outcomes and neonatal complications between COVID–19-vaccinated and unvaccinated pregnant women in Tabriz, Iran 伊朗大不里士接种新冠肺炎疫苗和未接种疫苗孕妇的妊娠结局和新生儿并发症的比较
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-03-15 DOI: 10.34172/ipp.2023.39520
S. Taghavi, M. Dehdilani, M. Dehdilani
Introduction: Insufficient information exists regarding the coronavirus disease 2019 (COVID-19) vaccination safety in pregnant women. Objectives: The present study compared the pregnancy and fetal outcomes among COVID-19-vaccinated and unvaccinated pregnant women. Patients and Methods: This cross-sectional study was conducted at Tabriz University of Medical Sciences (Tabriz, Iran) on 117 pregnant women (55 vaccinated women as the case group and 62 unvaccinated women as the control group) using census sampling in 2022. The maternal outcomes (death, admission to intensive care unit [ICU], premature birth, gestational hypertension, preeclampsia, oligohydramnios, and polyhydramnios) and neonatal outcomes (neonatal death, congenital disabilities, neonatal weight loss, admission to ICU, neonatal infection, neonatal fever, need for mechanical ventilation of the newborn, and one- and five-minute Apgar scores) were recorded. Then the two groups were compared using independent t-test, along with ANOVA and chi-square tests. Results: The number of women without pregnancy outcomes was marginally lower in the case group than in the control group (P=0.099). Gestational hypertension (P=0.312) and preterm birth were lower among unvaccinated women than among vaccinated women but this difference was not significant (P=0.089). Hospitalization in the neonatal intensive care unit (P=0.361), neonatal fever (P=0.259), and neonatal infection (P=0.079) were more common in infants born to vaccinated mothers than in infants born to unvaccinated women, although the difference not significant. Conclusion: Vaccination against COVID-19 appears to not be associated with an increased probability of maternal and neonatal complications.
简介:关于2019冠状病毒病(新冠肺炎)孕妇疫苗接种安全性的信息不足。目的:本研究比较了接种新冠肺炎疫苗和未接种疫苗的孕妇的妊娠和胎儿结局。患者和方法:这项横断面研究是在大不里士医学科学大学(伊朗大不里士)对117名孕妇(55名接种疫苗的妇女为病例组,62名未接种疫苗的女性为对照组)进行的,使用2022年的人口普查抽样。产妇结局(死亡、入住重症监护室、早产、妊娠期高血压、先兆子痫、羊水过少和羊水过多)和新生儿结局(新生儿死亡、先天性残疾、新生儿体重减轻、入住ICU、新生儿感染、新生儿发烧、新生儿需要机械通气以及1分钟和5分钟Apgar评分)分别为记录。然后使用独立t检验、方差分析和卡方检验对两组进行比较。结果:病例组无妊娠结局的妇女人数略低于对照组(P=0.099)。未接种疫苗的妇女的妊娠高血压(P=0.312)和早产率低于接种疫苗的女性,但这一差异并不显著(P=0.089)。新生儿重症监护室住院(P=0.361)、新生儿发烧(P=0.259),新生儿感染(P=0.079)在接种疫苗的母亲所生婴儿中比未接种疫苗的妇女所生婴儿更常见,尽管差异不显著。结论:接种新冠肺炎疫苗似乎与增加孕产妇和新生儿并发症的概率无关。
{"title":"Comparison of pregnancy outcomes and neonatal complications between COVID–19-vaccinated and unvaccinated pregnant women in Tabriz, Iran","authors":"S. Taghavi, M. Dehdilani, M. Dehdilani","doi":"10.34172/ipp.2023.39520","DOIUrl":"https://doi.org/10.34172/ipp.2023.39520","url":null,"abstract":"Introduction: Insufficient information exists regarding the coronavirus disease 2019 (COVID-19) vaccination safety in pregnant women. Objectives: The present study compared the pregnancy and fetal outcomes among COVID-19-vaccinated and unvaccinated pregnant women. Patients and Methods: This cross-sectional study was conducted at Tabriz University of Medical Sciences (Tabriz, Iran) on 117 pregnant women (55 vaccinated women as the case group and 62 unvaccinated women as the control group) using census sampling in 2022. The maternal outcomes (death, admission to intensive care unit [ICU], premature birth, gestational hypertension, preeclampsia, oligohydramnios, and polyhydramnios) and neonatal outcomes (neonatal death, congenital disabilities, neonatal weight loss, admission to ICU, neonatal infection, neonatal fever, need for mechanical ventilation of the newborn, and one- and five-minute Apgar scores) were recorded. Then the two groups were compared using independent t-test, along with ANOVA and chi-square tests. Results: The number of women without pregnancy outcomes was marginally lower in the case group than in the control group (P=0.099). Gestational hypertension (P=0.312) and preterm birth were lower among unvaccinated women than among vaccinated women but this difference was not significant (P=0.089). Hospitalization in the neonatal intensive care unit (P=0.361), neonatal fever (P=0.259), and neonatal infection (P=0.079) were more common in infants born to vaccinated mothers than in infants born to unvaccinated women, although the difference not significant. Conclusion: Vaccination against COVID-19 appears to not be associated with an increased probability of maternal and neonatal complications.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49316253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SARS-CoV-2 Omicron variant; psychological effect and influencing factors in elderly patients SARS-CoV-2染色体变体;老年患者的心理效应及影响因素
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-03-14 DOI: 10.34172/ipp.2023.39491
J. Eslami, Giti Setoodeh, Sara Dehghan, Y. Khanchemehr, Reyhaneh Sadeghian, Mahsa Asadollahi Hamedani, Mohamad Khaledi, Mobin Mohammadtabar, Najmeh Parsai
Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant appeared in South Africa for the first time. The high prevalence of its mortality in elderly patients has caused an increase in anxiety triggered by this disease in aged people. Objectives: The aim of this study is to evaluate the anxiety related-COVID-19 in the SARS-CoV-2 Omicron variant and its related factors in elderly patients who are candidates for cataract surgery. Patients and Methods: This descriptive-analytical study was conducted on 159 over 65 years old patients who are candidates for cataract surgery. Easy and accessible sampling methods were employed to select samples. Demographic characteristics sheet and the Corona Disease Anxiety Scale (CDAS) questionnaire were filled out by them. Fisher’s exact test, Mann-Whitney U and binary logistic regression tests were conducted for data analysis. Results: The results showed that out of 159 patients, 57.9% were female, with a mean age of 73.09 ± 9.64 years. The anxiety level of 70.4% of patients was mild or non-anxiety and 29.6% was moderate or severe. The correlation between anxiety related to COVID-19 with age, gender, vaccination status and history of being infected by COVID-19 was significant (P<0.05); however, the correlation with the job, place of residence and marital status were not significant (P>0.05). Conclusion: We concluded that older age, female gender, non-vaccinated and having no history of being infected by COVID-19 are the most influential factors that increase anxiety triggered by COVID-19 in elderly patients.
严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)欧米克隆变体首次在南非出现。老年患者的高死亡率增加了老年人因该病引起的焦虑。目的:本研究的目的是评估老年白内障手术候选者中SARS-CoV-2 Omicron变异中焦虑相关的covid -19及其相关因素。患者和方法:本描述性分析研究对159例65岁以上拟行白内障手术的患者进行了研究。采用简便易行的抽样方法选取样本。分别填写人口统计学特征表和冠状疾病焦虑量表(CDAS)。数据分析采用Fisher精确检验、Mann-Whitney U检验和二元logistic回归检验。结果:159例患者中,女性占57.9%,平均年龄73.09±9.64岁。70.4%的患者焦虑程度为轻度或无焦虑,29.6%的患者焦虑程度为中度或重度。与COVID-19相关的焦虑与年龄、性别、疫苗接种情况和感染史相关(P0.05)。结论:年龄较大、女性、未接种疫苗和无感染史是老年患者新冠肺炎焦虑增加的最重要因素。
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引用次数: 0
Investigating the expression of cytokeratin 5/6 in benign and malignant breast lesions 探讨细胞角蛋白5/6在乳腺良恶性病变中的表达
IF 0.8 Q3 Immunology and Microbiology Pub Date : 2023-02-27 DOI: 10.34172/ipp.2023.39477
Seyed Abbas Rezaei Naserabad, Shahram Bagheri, P. Kheradmand, S. Latifi
Introduction: Breast cancer (BC) is the most common cancer in women, which is the second most common malignancy in terms of mortality and prevalence after lung cancer. BC is a group of very diverse diseases that can be detected at a molecular, histopathological and clinical level. Objectives: The aim of the present study was to evaluate the expression of CK5/6 and its relationship to some of the factors affecting prognosis, such as tumor grade, lymph node involvement. Patients and Methods: In this cross-sectional study with descriptive and analytical aspects, 40 malignant and 20 benign tumors were collected in 2020 from the archives of the pathology department of two educational hospitals in the city of Ahvaz. The demographic and clinical characteristics of each specimen, including age, patient, lymphovascular and perineural invasion, lymph node involvement, tumor grade and tumor type were extracted from the patients’ record. Then the expression of CK5/6 intensity staining was examined by immunohistochemistry. Results: All benign breast lesions had positive expression for CK5/6 and staining intensity between six and nine. In the malignant group, 33 samples showed negative expression of CK5/6 and only seven samples (17.5%) showed positive expression with a low-staining index. A positive expression with a low-staining index. All seven positive specimens were invasive ductal carcinoma (IDC) lesions with staining index 2, 4, and 6. Additionally, none of the ductal carcinoma in situ (DCIS) specimens which immunostained were positive for CK5/6. In the present study, all IDCs with weak expression of CK5/6 were grade III. No statistically significant relationship was observed between perineural and lymphovascular invasion and lymph node involvement with the intensity of CK5/6 expression. Conclusion: Our study showed that cytokeratin immunohistochemical intensity is able to distinguish benign lesions from malignant IDC and DCIS lesions and accordingly in determining of tumor grade after weak staining in high-grade IDC, which may be due to squamous metaplasia in these tumors; however, more extensive research with a larger sample size are required to assess its effect in lymphovascular and perineural invasion and also lymph node involvement.
乳腺癌(BC)是女性中最常见的癌症,在死亡率和患病率方面是仅次于肺癌的第二常见恶性肿瘤。BC是一组非常多样化的疾病,可以在分子、组织病理学和临床水平上检测到。目的:本研究的目的是评估CK5/6的表达及其与肿瘤分级、淋巴结受累等影响预后的因素的关系。患者和方法:在这项具有描述性和分析性的横断面研究中,从阿瓦士市两所教育医院的病理学部门的档案中收集了2020年40例恶性肿瘤和20例良性肿瘤。从患者记录中提取每个标本的人口学和临床特征,包括年龄、患者、淋巴血管和神经周围浸润、淋巴结受累、肿瘤分级和肿瘤类型。免疫组化检测CK5/6强度染色的表达。结果:乳腺良性病变CK5/6均阳性表达,染色强度在6 ~ 9之间。恶性组CK5/6阴性表达33例,阳性表达7例(17.5%),低染色指数。低染色指数的阳性表达。7例阳性标本均为浸润性导管癌(invasive ductal carcinoma, IDC),染色指数分别为2、4和6。此外,免疫染色的导管原位癌(DCIS)标本均无CK5/6阳性。在本研究中,CK5/6弱表达的IDCs均为III级。CK5/6表达强度与神经周围、淋巴血管侵袭及淋巴结受累无统计学意义。结论:我们的研究表明,细胞角蛋白免疫组化强度能够区分恶性IDC和DCIS病变的良性病变,并据此判断高级别IDC弱染色后的肿瘤分级,这可能是由于这些肿瘤中存在鳞状化生;然而,需要更广泛的研究和更大的样本量来评估其在淋巴血管和神经周围侵袭以及淋巴结受累中的作用。
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引用次数: 1
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Immunopathologia Persa
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