Pub Date : 2023-12-07DOI: 10.20473/ijtid.v11i3.50859
Aditea Etnawati Putri, dr. sp.P.K., Betty Agustina Tambunan, Bambang Pujo Semedi, Narazah Mohd Yusoff, Siti Salmah Bt Noordin
Convalescent plasma (CP) may be an option for people with severe COVID-19. However, its efficiency remains unknown. A study was done to determine whether the levels of Anti-SARS-CoV-2 Spike Receptor-Binding Domain (S-RBD) antibodies and IL-10 in COVID-19 patients who had CP transfusion were related to their survival status. The observational cohort study included 40 patients with severe COVID-19 who were followed for 28 days after receiving a CP transfusion. Antibody and IL-10 levels were assessed on Day 1 before to CP transfusion and on Days 1, 2, and 7 following CP transfusion. Twenty six (65%) of the 40 patients survived. Anti-SARS-CoV-2 S-RBD antibody levels were observed to be significantly higher on Days 1, 2, and 7 following CP transfusion (p-value 0.05). Furthermore, IL-10 levels dropped significantly on Days 2 and 7 (p-value 0.05). However, neither the CT value nor the patients' survival status were linked to greater antibody levels or changes in IL-10 levels. According to the findings, CP transfusion can greatly enhance anti-SARS-CoV-2 S-RBD antibody levels while drastically decreasing IL-10 levels. These findings may have therapeutic implications for the use of CP as a COVID-19 therapy option. More research is needed to determine its efficacy in enhancing the survival rate of COVID-19 patients with severe symptoms.
{"title":"Examining convalescent plasma transfusion in severe COVID-19 patients, recent research highlights the significance of S-RBD antibodies and IL-10 levels","authors":"Aditea Etnawati Putri, dr. sp.P.K., Betty Agustina Tambunan, Bambang Pujo Semedi, Narazah Mohd Yusoff, Siti Salmah Bt Noordin","doi":"10.20473/ijtid.v11i3.50859","DOIUrl":"https://doi.org/10.20473/ijtid.v11i3.50859","url":null,"abstract":"Convalescent plasma (CP) may be an option for people with severe COVID-19. However, its efficiency remains unknown. A study was done to determine whether the levels of Anti-SARS-CoV-2 Spike Receptor-Binding Domain (S-RBD) antibodies and IL-10 in COVID-19 patients who had CP transfusion were related to their survival status. The observational cohort study included 40 patients with severe COVID-19 who were followed for 28 days after receiving a CP transfusion. Antibody and IL-10 levels were assessed on Day 1 before to CP transfusion and on Days 1, 2, and 7 following CP transfusion. Twenty six (65%) of the 40 patients survived. Anti-SARS-CoV-2 S-RBD antibody levels were observed to be significantly higher on Days 1, 2, and 7 following CP transfusion (p-value 0.05). Furthermore, IL-10 levels dropped significantly on Days 2 and 7 (p-value 0.05). However, neither the CT value nor the patients' survival status were linked to greater antibody levels or changes in IL-10 levels. According to the findings, CP transfusion can greatly enhance anti-SARS-CoV-2 S-RBD antibody levels while drastically decreasing IL-10 levels. These findings may have therapeutic implications for the use of CP as a COVID-19 therapy option. More research is needed to determine its efficacy in enhancing the survival rate of COVID-19 patients with severe symptoms.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.20473/ijtid.v11i3.46075
E. Setiawatie, P. Widiyanti, Retno Puji Rahayu, Alifiani Eprilina, Desi Sandrasari, Irma Yosephina Savitri, Lambang Bargowo, R. S. Meiliana
Periodontitis generаlly begins with gingivitis progresses to alveolar bone increаsing the risk of systemic diseаse. The primаry etiologicаl fаctor in the etiology of periodontаl diseаse is Аggregаtibаcter аctinomycetemcomitаns bacteria. Cetylpyridinium chloride (CPC) has a bactericidal effect by disrupting the function of bacterial membranes in the cytoplasm. CPC can also absorb negative charges from bacteria, increase bacterial cell wall permeability, decrease cell metabolism, and reduce bacterial attachment to teeth. Use of аntimicrobiаl toothpаste, such as Cetylpyridinium chloride (CPC), is one strategy to prevent periodontаl disease, but CPC is hаzаrdous in some quаntities. Аs а result, it should be compared to a natural toothpaste, specifically Nigellа sаtivа toothpаste. The objective of this study is to compare the аntibаcteriаl аctivity of nаturаl toothpаste contаining Nigellа sаtivа 3% with toothpаste contаining CPC 0.01% - 0.1% on Аggregаtibаcter аctinomycetemcomitаns. Experimentаl studies are used in this kind of research. The colony count method wаs used to аssess the nаturаl toothpаstes Nigellа sаtivа 3% аnd toothpаste contаining CPC 0.01- 0.1% for their cаpаcity in inhibiting the Аggregаtibаcter аctinomycetemcomitаns. Natural toothpаste containing Nigellа sаtivа 3% wаs completely inhibiting Аggregаtibаcter аctinomycetemcomitаns, compared to 0.01-0.1% CPC toothpаste. The significаnce level for the stаtisticаl test results wаs 0.000 (p<0.05). The conclution of this research are the nаturаl toothpаste contаining Nigellа sаtivа 3% аnd toothpаste contаining CPC 0.06%–0.1% can effectively suppress the growth of the microorgаnisms Аggregаtibаcter аctinomycetemcomitаns.
{"title":"Nigella sativa 3% Inhibition Test of Nаturаl Toothpаste Compаred Cetylpyridinum chloride (CPC) Toothpаste 0.01-0.1% on Аggregаtibаcter actinomycetemcomitаns","authors":"E. Setiawatie, P. Widiyanti, Retno Puji Rahayu, Alifiani Eprilina, Desi Sandrasari, Irma Yosephina Savitri, Lambang Bargowo, R. S. Meiliana","doi":"10.20473/ijtid.v11i3.46075","DOIUrl":"https://doi.org/10.20473/ijtid.v11i3.46075","url":null,"abstract":"Periodontitis generаlly begins with gingivitis progresses to alveolar bone increаsing the risk of systemic diseаse. The primаry etiologicаl fаctor in the etiology of periodontаl diseаse is Аggregаtibаcter аctinomycetemcomitаns bacteria. Cetylpyridinium chloride (CPC) has a bactericidal effect by disrupting the function of bacterial membranes in the cytoplasm. CPC can also absorb negative charges from bacteria, increase bacterial cell wall permeability, decrease cell metabolism, and reduce bacterial attachment to teeth. Use of аntimicrobiаl toothpаste, such as Cetylpyridinium chloride (CPC), is one strategy to prevent periodontаl disease, but CPC is hаzаrdous in some quаntities. Аs а result, it should be compared to a natural toothpaste, specifically Nigellа sаtivа toothpаste. The objective of this study is to compare the аntibаcteriаl аctivity of nаturаl toothpаste contаining Nigellа sаtivа 3% with toothpаste contаining CPC 0.01% - 0.1% on Аggregаtibаcter аctinomycetemcomitаns. Experimentаl studies are used in this kind of research. The colony count method wаs used to аssess the nаturаl toothpаstes Nigellа sаtivа 3% аnd toothpаste contаining CPC 0.01- 0.1% for their cаpаcity in inhibiting the Аggregаtibаcter аctinomycetemcomitаns. Natural toothpаste containing Nigellа sаtivа 3% wаs completely inhibiting Аggregаtibаcter аctinomycetemcomitаns, compared to 0.01-0.1% CPC toothpаste. The significаnce level for the stаtisticаl test results wаs 0.000 (p<0.05). The conclution of this research are the nаturаl toothpаste contаining Nigellа sаtivа 3% аnd toothpаste contаining CPC 0.06%–0.1% can effectively suppress the growth of the microorgаnisms Аggregаtibаcter аctinomycetemcomitаns.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.20473/ijtid.v11i3.44137
Alius Cahyadi, Angelina Yuwono, Nicholas Adrianto, Patricia Michelle Lukito
Purple Urine Bag Syndrome (PUBS) is a rare and benign clinical phenomenon associated with urinary tract infections, characterized by the distinctive purple discoloration of a urinary catheter bag. The underlying cause of PUBS is related to bacterial activity involved in the breakdown of tryptophan, mainly in individuals with predisposing factors such as long-term catheterization, constipation, alkaline urine, and being female. We present the case of a 76-year-old female patient with a history of tuberculous spondylitis who developed PUBS during her hospital stay. The patient had been bedridden for three years and had been given a urinary catheter, which was routinely replaced by a non-medical family member and without a proper antiseptic procedure. A urine culture revealed the presence of Proteus mirabilis, and the patient was treated with intravenous antibiotics while also replacing the urinary catheter and bag. After five days of treatment, the urine bag and catheter remained clear, and the patient was discharged. PUBS is a significant indicator of urinary tract infection, necessitating prompt medical intervention to prevent complications. Healthcare professionals should be aware of this syndrome to enable early detection and appropriate management.
{"title":"A Peculiar Manifestation of Urinary Tract Infection in a 76-year-old Female","authors":"Alius Cahyadi, Angelina Yuwono, Nicholas Adrianto, Patricia Michelle Lukito","doi":"10.20473/ijtid.v11i3.44137","DOIUrl":"https://doi.org/10.20473/ijtid.v11i3.44137","url":null,"abstract":"Purple Urine Bag Syndrome (PUBS) is a rare and benign clinical phenomenon associated with urinary tract infections, characterized by the distinctive purple discoloration of a urinary catheter bag. The underlying cause of PUBS is related to bacterial activity involved in the breakdown of tryptophan, mainly in individuals with predisposing factors such as long-term catheterization, constipation, alkaline urine, and being female. We present the case of a 76-year-old female patient with a history of tuberculous spondylitis who developed PUBS during her hospital stay. The patient had been bedridden for three years and had been given a urinary catheter, which was routinely replaced by a non-medical family member and without a proper antiseptic procedure. A urine culture revealed the presence of Proteus mirabilis, and the patient was treated with intravenous antibiotics while also replacing the urinary catheter and bag. After five days of treatment, the urine bag and catheter remained clear, and the patient was discharged. PUBS is a significant indicator of urinary tract infection, necessitating prompt medical intervention to prevent complications. Healthcare professionals should be aware of this syndrome to enable early detection and appropriate management.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139011774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.20473/ijtid.v11i3.44506
Wahyu Setyarini, Nurul Wiqoyah, Arif Nur, Muhammad Ansori
Syphilis, a common sexually transmitted disease, is caused by Treponema pallidum subsp. pallidum. Owing to the chameleonic behavior of syphilis, ocular involvement still presents a therapeutic problem. Direct detection of Treponema pallidum in the vitreous offers a potential diagnostic method because serodiagnosis has considerable limitations. The worldwide identification of T. pallidum substypes has occurred since the advent of molecular typing approaches. The purpose of this article is to provide more information on the development of a molecular approach for Treponema pallidum detection. A body of literature was gathered using automated database searches in Google Scholar, PubMed, and ScienceDirect. Although prior studies have focused on other genes, such as polA, 16S RNA, and the whole genome, there are still some that use the study of the arp and T. pallidum repeat (tpr) genes to subtype. Whole blood, vaginal ulcers, skin biopsies, and other samples can be used in molecular methods. Comparing quantitative reverse trascription-polymerase chain reaction (qRT-PCR) to traditional methods, such as reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody (IFA) assay, and virus isolation, qRT-PCR has the advantage of being faster and more sensitive. Quick molecular methods, particularly polymerase chain reaction (PCR) results, will enable early detection of primary, secondary, and latent syphilis, which will lead to prompt treatment and prevention of disease progression as well as a reduction in the amount of time that the patient's sexual partners are exposed to the illness.
{"title":"Molecular Diagnostic Tools for Treponema pallidum","authors":"Wahyu Setyarini, Nurul Wiqoyah, Arif Nur, Muhammad Ansori","doi":"10.20473/ijtid.v11i3.44506","DOIUrl":"https://doi.org/10.20473/ijtid.v11i3.44506","url":null,"abstract":"Syphilis, a common sexually transmitted disease, is caused by Treponema pallidum subsp. pallidum. Owing to the chameleonic behavior of syphilis, ocular involvement still presents a therapeutic problem. Direct detection of Treponema pallidum in the vitreous offers a potential diagnostic method because serodiagnosis has considerable limitations. The worldwide identification of T. pallidum substypes has occurred since the advent of molecular typing approaches. The purpose of this article is to provide more information on the development of a molecular approach for Treponema pallidum detection. A body of literature was gathered using automated database searches in Google Scholar, PubMed, and ScienceDirect. Although prior studies have focused on other genes, such as polA, 16S RNA, and the whole genome, there are still some that use the study of the arp and T. pallidum repeat (tpr) genes to subtype. Whole blood, vaginal ulcers, skin biopsies, and other samples can be used in molecular methods. Comparing quantitative reverse trascription-polymerase chain reaction (qRT-PCR) to traditional methods, such as reverse transcription-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody (IFA) assay, and virus isolation, qRT-PCR has the advantage of being faster and more sensitive. Quick molecular methods, particularly polymerase chain reaction (PCR) results, will enable early detection of primary, secondary, and latent syphilis, which will lead to prompt treatment and prevention of disease progression as well as a reduction in the amount of time that the patient's sexual partners are exposed to the illness.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139011823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.20473/ijtid.v11i3.48755
Nafisah Nurul Hapsari, Muhammad Ridho, Hafid Kurniawan, T. H. Sucipto, Nur Syamsiatul Fajar, Eduardus Bimo, Aksono Herupradopo, S. Fauziyah, Alicia Margaretta Widya, Febriana Aquaresta
Legionellosis is a respiratory infection caused by Legionella pneumophila, a bacterium that can infect protozoa and human lung cells. The disease can be mild or severe, and sometimes fatal, especially in immunocompromised people. Some types of antibiotics that can be used to treat Legionella disease are macrolides such as rifampicin, azithromycin and clarithromycin, as well as fluoroquinolones such as levofloxacin and moxifloxacin. The aim was to investigate the distribution and prevalence of Legionella in well water. This study collected and tested water samples from a different well water in Magetan Regency, East Java, Indonesia using DNA extraction, two-step PCR methods, and visualization with 1.5% agarose gel in UV transluminator. The results were visualized with QGIS 3.28.6 and compared with other tests. The results showed that none of the eight water samples were contaminated with L. pneumophila compared with positive control (403 bp).
{"title":"Screening of Legionella pneumophila from Well Water in Magetan Regency, Indonesia","authors":"Nafisah Nurul Hapsari, Muhammad Ridho, Hafid Kurniawan, T. H. Sucipto, Nur Syamsiatul Fajar, Eduardus Bimo, Aksono Herupradopo, S. Fauziyah, Alicia Margaretta Widya, Febriana Aquaresta","doi":"10.20473/ijtid.v11i3.48755","DOIUrl":"https://doi.org/10.20473/ijtid.v11i3.48755","url":null,"abstract":"Legionellosis is a respiratory infection caused by Legionella pneumophila, a bacterium that can infect protozoa and human lung cells. The disease can be mild or severe, and sometimes fatal, especially in immunocompromised people. Some types of antibiotics that can be used to treat Legionella disease are macrolides such as rifampicin, azithromycin and clarithromycin, as well as fluoroquinolones such as levofloxacin and moxifloxacin. The aim was to investigate the distribution and prevalence of Legionella in well water. This study collected and tested water samples from a different well water in Magetan Regency, East Java, Indonesia using DNA extraction, two-step PCR methods, and visualization with 1.5% agarose gel in UV transluminator. The results were visualized with QGIS 3.28.6 and compared with other tests. The results showed that none of the eight water samples were contaminated with L. pneumophila compared with positive control (403 bp).","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.20473/ijtid.v11i3.45516
Ika Nurvidha, Mahayanthi Mantra, I. Putu, Bayu Mayura, Ni Made, Adi Tarini
Streptococcus pneumoniae (S. pneumoniae) is a causative agent of pneumonia that can spread progressively, cause invasive disease, and increase mortality in humans. Pneumococcal or polysaccharide conjugate vaccination reduces pneumonia rates by vaccine-covered serotypes, but increases infection by non-vaccine serotypes. To determine the polysaccharide capsule serotype of S. pneumoniae isolates that cause infection at Prof. Dr. I.G.N.G. Ngoerah General Hospital and patterns of S. pneumoniae susceptibility to antibiotics from April 2017 to March 2022. All S. pneumoniae isolates from April 2017 and April 2022 were stored in STGG media in a freezer at -80 ℃ then subcultured on sheep blood agar. Polymerase Chain Reaction (PCR) was performed to determine pneumolysin and capsular polysaccharide serotypes of S. pneumoniae. Of the 22 isolates studied, the order of the number of serotypes from the highest was serotype 19F, 3, 6A/B, 33F, 15B/C, 4, and 6V. Seven isolates were untypeable. Antibiotic sensitivity pattern S. pneumoniae was found to be sensitive to linezolid 91%, vancomycin 86%, levofloxacin and benzylpenicillin 82%, ceftriaxone and clindamycin 73%, erythromycin 55%, and chloramphenicol 45%. Serotype 19F was identified as the most dominant capsular serotype; however, serotypes 33F and 15B/C were also found. Interestingly, the 33F serotype is not covered in the 13-valent pneumococcal conjugate vaccine (PCV13) but is covered in pneumococcal polysaccharide vaccine 23 (PPSV23), and the 15B/C serotype is not included in either PCV13 or PPSV23. The antimicrobial susceptibility patterns revealed that S. pneumoniae was susceptible to linezolid, vancomycin, benzylpenicillin, and levofloxacin.
{"title":"Polysaccharide Capsule Serotype and Antibiotic Susceptibility Pattern of Streptococcus pneumoniae Clinical Isolates in Bali","authors":"Ika Nurvidha, Mahayanthi Mantra, I. Putu, Bayu Mayura, Ni Made, Adi Tarini","doi":"10.20473/ijtid.v11i3.45516","DOIUrl":"https://doi.org/10.20473/ijtid.v11i3.45516","url":null,"abstract":"Streptococcus pneumoniae (S. pneumoniae) is a causative agent of pneumonia that can spread progressively, cause invasive disease, and increase mortality in humans. Pneumococcal or polysaccharide conjugate vaccination reduces pneumonia rates by vaccine-covered serotypes, but increases infection by non-vaccine serotypes. To determine the polysaccharide capsule serotype of S. pneumoniae isolates that cause infection at Prof. Dr. I.G.N.G. Ngoerah General Hospital and patterns of S. pneumoniae susceptibility to antibiotics from April 2017 to March 2022. All S. pneumoniae isolates from April 2017 and April 2022 were stored in STGG media in a freezer at -80 ℃ then subcultured on sheep blood agar. Polymerase Chain Reaction (PCR) was performed to determine pneumolysin and capsular polysaccharide serotypes of S. pneumoniae. Of the 22 isolates studied, the order of the number of serotypes from the highest was serotype 19F, 3, 6A/B, 33F, 15B/C, 4, and 6V. Seven isolates were untypeable. Antibiotic sensitivity pattern S. pneumoniae was found to be sensitive to linezolid 91%, vancomycin 86%, levofloxacin and benzylpenicillin 82%, ceftriaxone and clindamycin 73%, erythromycin 55%, and chloramphenicol 45%. Serotype 19F was identified as the most dominant capsular serotype; however, serotypes 33F and 15B/C were also found. Interestingly, the 33F serotype is not covered in the 13-valent pneumococcal conjugate vaccine (PCV13) but is covered in pneumococcal polysaccharide vaccine 23 (PPSV23), and the 15B/C serotype is not included in either PCV13 or PPSV23. The antimicrobial susceptibility patterns revealed that S. pneumoniae was susceptible to linezolid, vancomycin, benzylpenicillin, and levofloxacin.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138984000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.20473/ijtid.v11i3.45942
Mukti Sadewo, H. Sukmaningtyas, Agus Priambodo, C. Prihharsanti
The high prevalence and mortality of tuberculosis (TB) is serious threats to the world. Tuberculous spondylitis accounts for 50% of all bone and joint TB cases. The accuracy of diagnosis to detect disease severity using Magnetic Resonance Imaging (MRI) modalities is important when considering potential risk factors. This study aimed to analyze the association of risk factors, including age, sex, spinal lesion location, and abscess location, with the severity of tuberculous spondylitis based on MRI modality using the Gulhane Askari Tip Akademisi (GATA) classification. This study had a cross-sectional design. The study sample consisted of 50 patients who met the inclusion criteria and underwent MRI. The statistical analysis performed in this study was multivariate analysis using multiple linear regression. The results showed that 84% (n = 42) of tuberculous spondylitis affected patients aged 18-65 years, and 70% (n = 35) of cases were found in female patients. In addition, 42% (n = 21) of cases were classified as tuberculous spondylitis grade III according to the GATA classification and were the most common cases in this study. Statistical tests showed no association between age, the location of spinal lesions, and the degree of tuberculous spondylitis. However, there was an association between sex, spinal abscess location, and degree of tuberculous spondylitis. There was no significant association between age and spinal lesion location or degree of tuberculous spondylitis. However, there was a significant association between sex, spinal abscess location, and the degree of tuberculous spondylitis.
结核病（TB）的高发病率和高死亡率严重威胁着世界。结核性脊柱炎占所有骨关节结核病例的 50%。考虑到潜在的风险因素，使用磁共振成像（MRI）模式检测疾病严重程度的诊断准确性非常重要。本研究旨在使用 Gulhane Askari Tip Akademisi（GATA）分类法，根据磁共振成像模式分析年龄、性别、脊柱病变位置和脓肿位置等风险因素与结核性脊柱炎严重程度的关系。本研究采用横断面设计。研究样本包括 50 名符合纳入标准并接受磁共振成像检查的患者。本研究采用多元线性回归进行多变量统计分析。结果显示，84%（n = 42）的结核性脊柱炎患者年龄在 18-65 岁之间，70%（n = 35）的病例为女性患者。此外，根据 GATA 分类法，42%（n = 21）的病例被归类为结核性脊柱炎 III 级，是本研究中最常见的病例。统计检验显示，年龄、脊柱病变的位置与结核性脊柱炎的程度之间没有关联。然而，性别、脊柱脓肿位置和结核性脊柱炎程度之间存在关联。年龄与脊柱病变位置或结核性脊柱炎程度无明显关联。然而，性别、脊柱脓肿位置和结核性脊柱炎程度之间存在明显关联。
{"title":"Risk Factors Influencing the Degree of Tuberculous Spondylitis Based on MRI Modality","authors":"Mukti Sadewo, H. Sukmaningtyas, Agus Priambodo, C. Prihharsanti","doi":"10.20473/ijtid.v11i3.45942","DOIUrl":"https://doi.org/10.20473/ijtid.v11i3.45942","url":null,"abstract":"The high prevalence and mortality of tuberculosis (TB) is serious threats to the world. Tuberculous spondylitis accounts for 50% of all bone and joint TB cases. The accuracy of diagnosis to detect disease severity using Magnetic Resonance Imaging (MRI) modalities is important when considering potential risk factors. This study aimed to analyze the association of risk factors, including age, sex, spinal lesion location, and abscess location, with the severity of tuberculous spondylitis based on MRI modality using the Gulhane Askari Tip Akademisi (GATA) classification. This study had a cross-sectional design. The study sample consisted of 50 patients who met the inclusion criteria and underwent MRI. The statistical analysis performed in this study was multivariate analysis using multiple linear regression. The results showed that 84% (n = 42) of tuberculous spondylitis affected patients aged 18-65 years, and 70% (n = 35) of cases were found in female patients. In addition, 42% (n = 21) of cases were classified as tuberculous spondylitis grade III according to the GATA classification and were the most common cases in this study. Statistical tests showed no association between age, the location of spinal lesions, and the degree of tuberculous spondylitis. However, there was an association between sex, spinal abscess location, and degree of tuberculous spondylitis. There was no significant association between age and spinal lesion location or degree of tuberculous spondylitis. However, there was a significant association between sex, spinal abscess location, and the degree of tuberculous spondylitis.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139011663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.20473/ijtid.v11i3.47664
G. Putu, Suka Aryana, Ni Nyoman, Sri Budayanti, N. Putu, Harta Wedari
Clostridium difficile infection (CDI) is a typical healthcare-associated infection that contributes to a significant proportion of morbidity and mortality among hospitalized patients. Anaerobe microbiological laboratory examinations cannot be performed at Prof. Dr. I.G.N.G. Ngoerah Hospital, leading to many undetected cases; therefore, it is crucial to determine the risk factors. Identification of cases may help to prevent, recognize, and appropriately treat CDI, reducing morbidity and mortality. This study aimed to determine the risk factors associated with suspected CDI in elderly patients with diarrhea due to their vulnerability to immunosenescence. This research used medical records as secondary data to determine the risk factors associated with suspected CDI in elderly diarrhea patients at Prof. Dr. I.G.N.G. Ngoerah Hospital during the year period of 2017 to 2021 with total of 70 samples who met inclusion criteria by purposive sampling technique. One hundred percent of all samples had a history of hospitalization for ³ 48 h. There were 53 patients (77.1%) had a history of antibiotic use, 26 (37.1%) proton pump inhibitors (PPIs), 66 (94.3%) chemotherapy, 66 (94.3%) urinary tract infections, 6 (8.6%) kidney disease, and 7 (10%) myocardial infarction. Seven (10%) patients had vascular disease, and 11 (15.7%) had diabetes mellitus. Risk factors associated with suspected CDI in elderly patients with diarrhea at Prof. Dr. I.G.N.G. Ngoerah Hospital include hospitalization ³ for 48 hours, use of antibiotics in the last few months, proton pump inhibitors, decreased immune system, urinary tract infection, kidney disease, myocardial infarction, vascular disease, and diabetes mellitus.
{"title":"Risk Factors Associated with Suspected Clostridium difficile Infection (CDI) in Elderly Diarrhea Patients at Prof. Dr. I.G.N.G. Ngoerah Hospital","authors":"G. Putu, Suka Aryana, Ni Nyoman, Sri Budayanti, N. Putu, Harta Wedari","doi":"10.20473/ijtid.v11i3.47664","DOIUrl":"https://doi.org/10.20473/ijtid.v11i3.47664","url":null,"abstract":"Clostridium difficile infection (CDI) is a typical healthcare-associated infection that contributes to a significant proportion of morbidity and mortality among hospitalized patients. Anaerobe microbiological laboratory examinations cannot be performed at Prof. Dr. I.G.N.G. Ngoerah Hospital, leading to many undetected cases; therefore, it is crucial to determine the risk factors. Identification of cases may help to prevent, recognize, and appropriately treat CDI, reducing morbidity and mortality. This study aimed to determine the risk factors associated with suspected CDI in elderly patients with diarrhea due to their vulnerability to immunosenescence. This research used medical records as secondary data to determine the risk factors associated with suspected CDI in elderly diarrhea patients at Prof. Dr. I.G.N.G. Ngoerah Hospital during the year period of 2017 to 2021 with total of 70 samples who met inclusion criteria by purposive sampling technique. One hundred percent of all samples had a history of hospitalization for ³ 48 h. There were 53 patients (77.1%) had a history of antibiotic use, 26 (37.1%) proton pump inhibitors (PPIs), 66 (94.3%) chemotherapy, 66 (94.3%) urinary tract infections, 6 (8.6%) kidney disease, and 7 (10%) myocardial infarction. Seven (10%) patients had vascular disease, and 11 (15.7%) had diabetes mellitus. Risk factors associated with suspected CDI in elderly patients with diarrhea at Prof. Dr. I.G.N.G. Ngoerah Hospital include hospitalization ³ for 48 hours, use of antibiotics in the last few months, proton pump inhibitors, decreased immune system, urinary tract infection, kidney disease, myocardial infarction, vascular disease, and diabetes mellitus.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-08DOI: 10.20473/ijtid.v11i2.37328
Nina Difla Muflikhah
Dengue Haemorrhagic Fever (DHF) is one of the major public health problems in Indonesia. As the population density increases, the number of sufferers increases. Aedes aegypti mosquitoes are vectors for the disease. The absence of drugs make the best prevention effort by eradicating mosquito nests, killing larvae and adult mosquitoes. Mulberry leaves (Morus alba L.) may be used as larvacides in the presence of chemical compounds of flavonoids and saponins that inhibit feeding and disrupt the process of insect metabolism. The purpose of this research has to determine the effect of mulberry leaf extract (Morus alba L.), to determine the larvicide effect of mulberry leaf extract (Morus alba L.) and to determine the concentration of mulberry leaf extract (Morus alba L.) which is optimal in killing third instar Aedes aegypti larvae. This research used Randomized Design Group (RDG) method with treatment consisted 4 concentrations (0.25%, 0.5%, 0.75%, and 1%), negative control and positive control (ABATE) with 6 repetitions. The results of probit analysis showed that LC50 values were 1.124% and LC90 was 4.413%. From the one way ANOVA test at each concentration of 0.25%, 0.5%, 0.75%, and 1%, the F count result is 208.331, the value was greater than F table which is 2.53 and the significant value is 0.000 (sig <0.05) then mulberry leaf extract (Morus alba L.) has a affected to eliminated of Aedes aegypti larvae. Conclusion from the results of the one way ANOVA test of mulberry leaf extract (Morus alba L.) was affected to eliminated third instar Aedes aegypti larvae
出血性登革热（DHF）是印度尼西亚的主要公共卫生问题之一。随着人口密度的增加，患者人数也在增加。埃及伊蚊是登革热的病媒。在没有药物的情况下，最好的预防办法就是消灭蚊巢、杀死幼虫和成蚊。桑叶（Morus alba L.）含有黄酮类和皂苷类化合物，可抑制进食并破坏昆虫的新陈代谢过程，因此可用作杀幼虫剂。本研究的目的是确定桑叶提取物（白桑）的作用，确定桑叶提取物（白桑）的杀幼虫剂效果，并确定桑叶提取物（白桑）杀死埃及伊蚊三龄幼虫的最佳浓度。该研究采用随机设计组（RDG）方法，处理包括 4 种浓度（0.25%、0.5%、0.75% 和 1%）、阴性对照和阳性对照（ABATE），共重复 6 次。probit 分析结果表明，LC50 值为 1.124%，LC90 为 4.413%。在 0.25%、0.5%、0.75% 和 1%的浓度下进行单因素方差分析，F 值为 208.331，大于 F 表中的 2.53，显著值为 0.000（sig <0.05），则桑叶提取物（Morus alba L.）对埃及伊蚊幼虫有抑制作用。根据桑叶提取物对消灭埃及伊蚊第三龄幼虫的影响的单因素方差分析结果得出结论
{"title":"Larvacidal Activity of the Mulberry (Morus alba L.) Leaf Extract Against Larvae of Aedes aegypti","authors":"Nina Difla Muflikhah","doi":"10.20473/ijtid.v11i2.37328","DOIUrl":"https://doi.org/10.20473/ijtid.v11i2.37328","url":null,"abstract":"Dengue Haemorrhagic Fever (DHF) is one of the major public health problems in Indonesia. As the population density increases, the number of sufferers increases. Aedes aegypti mosquitoes are vectors for the disease. The absence of drugs make the best prevention effort by eradicating mosquito nests, killing larvae and adult mosquitoes. Mulberry leaves (Morus alba L.) may be used as larvacides in the presence of chemical compounds of flavonoids and saponins that inhibit feeding and disrupt the process of insect metabolism. The purpose of this research has to determine the effect of mulberry leaf extract (Morus alba L.), to determine the larvicide effect of mulberry leaf extract (Morus alba L.) and to determine the concentration of mulberry leaf extract (Morus alba L.) which is optimal in killing third instar Aedes aegypti larvae. This research used Randomized Design Group (RDG) method with treatment consisted 4 concentrations (0.25%, 0.5%, 0.75%, and 1%), negative control and positive control (ABATE) with 6 repetitions. The results of probit analysis showed that LC50 values were 1.124% and LC90 was 4.413%. From the one way ANOVA test at each concentration of 0.25%, 0.5%, 0.75%, and 1%, the F count result is 208.331, the value was greater than F table which is 2.53 and the significant value is 0.000 (sig <0.05) then mulberry leaf extract (Morus alba L.) has a affected to eliminated of Aedes aegypti larvae. Conclusion from the results of the one way ANOVA test of mulberry leaf extract (Morus alba L.) was affected to eliminated third instar Aedes aegypti larvae","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-08DOI: 10.20473/ijtid.v11i2.43104
P. Widiyanti, Tarissa Diandra Putri, Wibowo, Andhi Baskoro, Siswanto
Blood bag is a medical device that stokes and transports whole blood or blood components. The material that is often used for blood bag membranes is Polyvinyl Chloride (PVC), however the common problem that is bacterial contamination and that material have not antibacterial characteristic. Because of this matter, the aim of this reseach are a blood bag that has antibacterial function is needed and meet the ideal standard as bloodbag. Chitosan as a blood bag membrane material fabrication to get the antibacterial effect. Chitosan is chosen as a blood bag material fabrication to get the antibacterial effect. Chitosan has several specific biocompatibility properties, antibacterial, chelation, and biodegradability. This study used various Chitosan concentrations of 1.5%, 2%, 2.5%, and 3%, and Glycerol was added as a plasticizer. The composition of Chitosan: Glycerol is 1:1. Then, the mixture is added to the PVC solution in a ratio of 1:5 then poured into a petri dish. The results showed characterization that the biocomposite PVC-Glycerol with the addition of 3% concentration of chitosan was the best composition, the tensile strength test result of biocomposite is 21.20 MPa, the absence of membrane pores in the morphology of the blood bag, the hemolytic activity is 0.24%, and the inhibition zones of E. coli and S. aureus, respectively 11.66 mm and 12.66 mm in diameter. Based on the characterization results, the biocomposite PVC-Glycerol membrane with the addition of Chitosan has a very high potential as a candidate for blood bag membranes
{"title":"Polyvinyl Chloride (PVC)-Glycerol with Chitosan Addition for Antibacterial Blood Bag Application","authors":"P. Widiyanti, Tarissa Diandra Putri, Wibowo, Andhi Baskoro, Siswanto","doi":"10.20473/ijtid.v11i2.43104","DOIUrl":"https://doi.org/10.20473/ijtid.v11i2.43104","url":null,"abstract":"Blood bag is a medical device that stokes and transports whole blood or blood components. The material that is often used for blood bag membranes is Polyvinyl Chloride (PVC), however the common problem that is bacterial contamination and that material have not antibacterial characteristic. Because of this matter, the aim of this reseach are a blood bag that has antibacterial function is needed and meet the ideal standard as bloodbag. Chitosan as a blood bag membrane material fabrication to get the antibacterial effect. Chitosan is chosen as a blood bag material fabrication to get the antibacterial effect. Chitosan has several specific biocompatibility properties, antibacterial, chelation, and biodegradability. This study used various Chitosan concentrations of 1.5%, 2%, 2.5%, and 3%, and Glycerol was added as a plasticizer. The composition of Chitosan: Glycerol is 1:1. Then, the mixture is added to the PVC solution in a ratio of 1:5 then poured into a petri dish. The results showed characterization that the biocomposite PVC-Glycerol with the addition of 3% concentration of chitosan was the best composition, the tensile strength test result of biocomposite is 21.20 MPa, the absence of membrane pores in the morphology of the blood bag, the hemolytic activity is 0.24%, and the inhibition zones of E. coli and S. aureus, respectively 11.66 mm and 12.66 mm in diameter. Based on the characterization results, the biocomposite PVC-Glycerol membrane with the addition of Chitosan has a very high potential as a candidate for blood bag membranes","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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